AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene28571

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28571.t1 MTR_8g079660 87.879 264 32 0 6 269 1 264 3.17e-166 461
MS.gene28571.t1 MTR_8g079620 67.537 268 46 3 6 271 1 229 1.49e-122 349
MS.gene28571.t1 MTR_8g079580 52.601 173 76 4 6 176 1 169 1.10e-51 169
MS.gene28571.t1 MTR_3g073690 52.727 110 52 0 13 122 9 118 9.18e-39 134
MS.gene28571.t1 MTR_5g017950 44.654 159 75 3 13 158 8 166 2.96e-38 137
MS.gene28571.t1 MTR_3g452660 51.304 115 55 1 13 126 7 121 1.01e-36 132
MS.gene28571.t1 MTR_6g005080 45.192 104 57 0 13 116 10 113 2.55e-31 113
MS.gene28571.t1 MTR_2g093310 46.226 106 57 0 8 113 28 133 7.41e-31 114
MS.gene28571.t1 MTR_4g105170 45.192 104 57 0 13 116 11 114 2.99e-30 112
MS.gene28571.t1 MTR_7g033800 44.340 106 59 0 13 118 11 116 5.52e-30 111
MS.gene28571.t1 MTR_6g005070 45.631 103 56 0 13 115 9 111 2.32e-29 108
MS.gene28571.t1 MTR_3g077240 45.192 104 57 0 13 116 10 113 2.41e-29 110
MS.gene28571.t1 MTR_2g068760 44.660 103 57 0 11 113 11 113 1.60e-28 107
MS.gene28571.t1 MTR_3g031660 43.564 101 57 0 13 113 7 107 1.70e-28 107
MS.gene28571.t1 MTR_3g094690 46.226 106 57 0 11 116 45 150 2.08e-28 108
MS.gene28571.t1 MTR_5g083960 45.192 104 57 0 13 116 39 142 2.44e-28 107
MS.gene28571.t1 MTR_5g083230 45.545 101 55 0 13 113 6 106 3.10e-28 106
MS.gene28571.t1 MTR_4g060950 44.340 106 58 1 9 113 20 125 3.23e-28 107
MS.gene28571.t1 MTR_4g083680 46.000 100 54 0 13 112 15 114 6.98e-28 105
MS.gene28571.t1 MTR_8g040900 47.115 104 55 0 13 116 7 110 9.07e-28 107
MS.gene28571.t1 MTR_3g071590 44.554 101 56 0 13 113 8 108 2.09e-27 103
MS.gene28571.t1 MTR_3g094690 47.170 106 55 1 11 116 45 149 3.45e-27 105
MS.gene28571.t1 MTR_1g095850 46.000 100 54 0 14 113 73 172 8.51e-27 104
MS.gene28571.t1 MTR_5g075020 42.609 115 66 0 11 125 41 155 1.91e-26 103
MS.gene28571.t1 MTR_5g083010 46.078 102 55 0 14 115 12 113 2.45e-26 102
MS.gene28571.t1 MTR_5g075020 42.609 115 66 0 11 125 45 159 2.86e-26 103
MS.gene28571.t1 MTR_4g099230 45.098 102 55 1 14 114 3 104 1.84e-24 96.3
MS.gene28571.t1 MTR_7g028905 37.736 106 66 0 9 114 1 106 3.93e-24 94.4
MS.gene28571.t1 MTR_5g093010 37.736 106 66 0 9 114 1 106 6.61e-24 94.4
MS.gene28571.t1 MTR_3g071420 46.392 97 52 0 14 110 15 111 6.81e-24 95.5
MS.gene28571.t1 MTR_6g027710 42.857 105 59 1 10 113 37 141 7.94e-24 96.7
MS.gene28571.t1 MTR_6g027700 39.623 106 62 2 6 110 1 105 6.16e-22 91.3
MS.gene28571.t1 MTR_7g096530 41.748 103 59 1 13 114 14 116 7.09e-22 91.3
MS.gene28571.t1 MTR_8g036085 38.095 105 64 1 13 116 23 127 1.65e-20 87.8
MS.gene28571.t1 MTR_1g070220 40.594 101 59 1 13 112 15 115 2.12e-20 87.0
MS.gene28571.t1 MTR_7g074990 40.196 102 60 1 13 113 20 121 4.16e-20 86.7
MS.gene28571.t1 MTR_8g036105 42.718 103 58 1 13 114 18 120 5.70e-20 85.9
MS.gene28571.t1 MTR_6g011250 39.216 102 61 1 14 114 10 111 2.28e-19 83.6
MS.gene28571.t1 MTR_8g017090 34.286 105 69 0 12 116 11 115 2.63e-19 85.9
MS.gene28571.t1 MTR_6g011200 36.697 109 65 2 10 114 3 111 1.96e-18 82.4
MS.gene28571.t1 MTR_5g085390 41.284 109 60 2 10 114 3 111 3.41e-18 81.6
MS.gene28571.t1 MTR_1g070205 37.500 104 64 1 13 115 12 115 6.20e-18 80.9
MS.gene28571.t1 MTR_6g011230 34.211 114 68 1 12 118 10 123 2.41e-17 78.6
MS.gene28571.t1 MTR_4g107450 36.275 102 65 0 12 113 9 110 3.08e-17 80.1
MS.gene28571.t1 MTR_2g100020 32.039 103 70 0 12 114 18 120 3.94e-17 79.3
MS.gene28571.t1 MTR_7g096610 34.615 104 67 1 13 115 8 111 1.04e-16 77.4
MS.gene28571.t1 MTR_7g075230 38.095 105 62 2 13 114 12 116 5.84e-16 74.7
MS.gene28571.t1 MTR_5g080470 34.314 102 66 1 14 114 8 109 3.72e-15 72.4
MS.gene28571.t1 MTR_4g088035 33.721 86 57 0 14 99 7 92 6.02e-13 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28571.t1 AT5G66870 43.312 157 86 2 13 167 7 162 2.55e-37 134
MS.gene28571.t1 AT2G23660 49.091 110 56 0 13 122 5 114 2.20e-33 124
MS.gene28571.t1 AT2G23660 49.091 110 56 0 13 122 5 114 2.20e-33 124
MS.gene28571.t1 AT2G23660 49.091 110 56 0 13 122 5 114 2.20e-33 124
MS.gene28571.t1 AT1G07900 46.226 106 57 0 8 113 28 133 3.70e-31 114
MS.gene28571.t1 AT3G27650 44.248 113 63 0 4 116 30 142 8.11e-31 113
MS.gene28571.t1 AT5G63090 46.154 104 56 0 13 116 11 114 3.52e-30 112
MS.gene28571.t1 AT5G63090 46.154 104 56 0 13 116 11 114 3.52e-30 112
MS.gene28571.t1 AT5G63090 46.154 104 56 0 13 116 11 114 3.52e-30 112
MS.gene28571.t1 AT5G63090 46.154 104 56 0 13 116 11 114 3.52e-30 112
MS.gene28571.t1 AT1G65620 50.000 106 53 0 13 118 9 114 5.32e-30 112
MS.gene28571.t1 AT1G65620 50.000 106 53 0 13 118 9 114 5.32e-30 112
MS.gene28571.t1 AT1G65620 50.000 106 53 0 13 118 9 114 5.32e-30 112
MS.gene28571.t1 AT1G65620 50.000 106 53 0 13 118 9 114 5.32e-30 112
MS.gene28571.t1 AT1G65620 50.000 106 53 0 13 118 9 114 5.32e-30 112
MS.gene28571.t1 AT2G28500 45.714 105 57 0 9 113 51 155 8.22e-30 112
MS.gene28571.t1 AT2G30130 45.545 101 55 0 13 113 8 108 7.32e-29 108
MS.gene28571.t1 AT2G30340 44.340 106 59 0 11 116 51 156 8.17e-28 108
MS.gene28571.t1 AT2G30340 44.340 106 59 0 11 116 50 155 8.17e-28 108
MS.gene28571.t1 AT2G40470 44.828 116 64 0 11 126 18 133 1.15e-27 105
MS.gene28571.t1 AT2G40470 44.828 116 64 0 11 126 43 158 2.78e-27 105
MS.gene28571.t1 AT1G16530 43.269 104 58 1 11 113 12 115 6.65e-27 103
MS.gene28571.t1 AT3G26620 40.594 101 60 0 14 114 6 106 2.35e-26 100
MS.gene28571.t1 AT3G26660 40.594 101 60 0 14 114 6 106 2.43e-26 100
MS.gene28571.t1 AT1G31320 47.727 88 46 0 13 100 13 100 1.37e-25 99.8
MS.gene28571.t1 AT3G11090 42.000 100 58 0 14 113 12 111 1.00e-23 94.7
MS.gene28571.t1 AT3G50510 38.318 107 66 0 10 116 9 115 2.29e-22 92.0
MS.gene28571.t1 AT3G50510 38.318 107 66 0 10 116 9 115 2.29e-22 92.0
MS.gene28571.t1 AT4G00210 43.689 103 57 1 13 114 11 113 6.04e-22 91.3
MS.gene28571.t1 AT1G72980 40.000 100 60 0 14 113 14 113 8.57e-22 90.9
MS.gene28571.t1 AT5G35900 38.462 104 64 0 14 117 6 109 2.66e-21 89.4
MS.gene28571.t1 AT3G03760 42.157 102 58 1 13 113 51 152 4.18e-21 90.5
MS.gene28571.t1 AT3G47870 37.143 105 66 0 14 118 37 141 1.93e-20 89.4
MS.gene28571.t1 AT2G42430 37.143 105 65 1 13 116 15 119 2.13e-19 85.1
MS.gene28571.t1 AT3G13850 34.951 103 67 0 14 116 37 139 1.29e-18 83.6
MS.gene28571.t1 AT4G00220 39.000 100 60 1 16 114 20 119 1.61e-18 82.4
MS.gene28571.t1 AT3G58190 36.538 104 65 1 13 115 11 114 1.24e-17 79.7
MS.gene28571.t1 AT2G45410 40.000 100 59 1 16 114 19 118 2.43e-17 78.2
MS.gene28571.t1 AT2G45420 37.000 100 62 1 16 114 40 139 1.16e-16 77.8
MS.gene28571.t1 AT2G42440 33.654 104 68 1 13 115 7 110 1.29e-16 77.4
MS.gene28571.t1 AT1G06280 39.241 79 48 0 12 90 23 101 1.20e-15 73.9
MS.gene28571.t1 AT2G31310 29.808 104 72 1 13 115 7 110 3.68e-14 69.7
MS.gene28571.t1 AT4G22700 34.921 126 78 4 14 136 6 130 5.14e-14 69.3
MS.gene28571.t1 AT5G06080 31.429 105 71 1 13 116 7 111 5.98e-14 68.9

Find 81 sgRNAs with CRISPR-Local

Find 82 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATGTTCTTGTTCGTGATTC+TGG 0.069191 8.4:-27125651 MS.gene28571:CDS
CTTCTGTGCTTCCTTCTTCT+AGG 0.193943 8.4:-27125581 MS.gene28571:CDS
ATCTCTCTTATTATTCTTGT+TGG 0.231044 8.4:+27125526 None:intergenic
TAATATCGCATGGCATTGTT+AGG 0.339594 8.4:-27126441 None:intergenic
ATGTTCTTGTTCGTGATTCT+GGG 0.345951 8.4:-27125650 MS.gene28571:CDS
ATCAAGGTACACTCTGTGTT+TGG 0.360225 8.4:-27126203 MS.gene28571:CDS
CTACCTTGAAGGTCATTTGC+TGG 0.366573 8.4:+27126059 None:intergenic
TGAACAAGTTATTGAATCAT+TGG 0.367700 8.4:-27126132 MS.gene28571:CDS
GACCTGAGGACTGAGGTATT+TGG 0.383512 8.4:+27125989 None:intergenic
GGCTTAAAGCTGAAACAATC+AGG 0.394378 8.4:+27125945 None:intergenic
TTAAAACAAAAGCTTCTTAG+TGG 0.403319 8.4:-27125819 MS.gene28571:CDS
TTCTGTGCTTCCTTCTTCTA+GGG 0.409577 8.4:-27125580 MS.gene28571:CDS
GTTCGTGATTCTGGGTTGGC+AGG 0.416595 8.4:-27125642 MS.gene28571:CDS
GTGGAACGTCAGGAAGCATC+TGG 0.419094 8.4:+27125616 None:intergenic
AGGTTGAGGGACCCAGTCTA+TGG 0.429899 8.4:-27126092 MS.gene28571:CDS
GGACCTGCACAGCCATAGAC+TGG 0.430008 8.4:+27126080 None:intergenic
TTGACATAACCACCACCAAC+AGG 0.431936 8.4:+27125711 None:intergenic
ATAGTCGGTGCAAATGCATT+TGG 0.441386 8.4:+27125924 None:intergenic
ATGAAACGCTCTGGGTTGTC+AGG 0.443549 8.4:+27126224 None:intergenic
GACAGTCGGTGCAAGACAAT+TGG 0.445989 8.4:-27125901 MS.gene28571:CDS
GACCCAGTCTATGGCTGTGC+AGG 0.446474 8.4:-27126083 MS.gene28571:CDS
CAAGAACATTAGCACCCATC+TGG 0.449123 8.4:+27125664 None:intergenic
GCCAACCCATCATCACCATC+GGG 0.452840 8.4:+27125795 None:intergenic
CTTCATTCAACCTACCTTGA+AGG 0.457276 8.4:+27126048 None:intergenic
AGTGGCCCCGATGGTGATGA+TGG 0.469173 8.4:-27125801 MS.gene28571:CDS
CAGGAACATTAACATCCATC+TGG 0.471455 8.4:+27125766 None:intergenic
GGGTTGTCAGGTGGAAAGTA+AGG 0.475348 8.4:+27126236 None:intergenic
GCATCAAGATGTGGAACGTC+AGG 0.482049 8.4:+27125606 None:intergenic
GTTGGTGGTGGTTATGTCAA+TGG 0.494229 8.4:-27125708 MS.gene28571:CDS
TGTACCTTGATGAAACGCTC+TGG 0.497575 8.4:+27126215 None:intergenic
AGATGATTGCACGCTGCGCA+CGG 0.500304 8.4:+27126293 None:intergenic
TGCCAACCCATCATCACCAT+CGG 0.501173 8.4:+27125794 None:intergenic
TGAGGCTGAGGCCAGGTTGA+GGG 0.503784 8.4:-27126105 MS.gene28571:CDS
TATTGAATCATTGGTGTATG+AGG 0.512390 8.4:-27126123 MS.gene28571:CDS
GGCCAAATACCTCAGTCCTC+AGG 0.517957 8.4:-27125991 MS.gene28571:CDS
AGGTCCCACCCTGTTGGTGG+TGG 0.520212 8.4:-27125720 MS.gene28571:CDS
ATGAGGCTGAGGCCAGGTTG+AGG 0.520828 8.4:-27126106 MS.gene28571:CDS
GATGGTGATGATGGGTTGGC+AGG 0.524172 8.4:-27125792 MS.gene28571:CDS
CTAGAAGAAGGAAGCACAGA+AGG 0.524800 8.4:+27125582 None:intergenic
AGCGTGCAATCATCTCCGTC+AGG 0.528327 8.4:-27126285 MS.gene28571:CDS
GTTAATGTTCCTGTTGAAAA+CGG 0.528434 8.4:-27125756 MS.gene28571:CDS
ACTAGGTCCCACCCTGTTGG+TGG 0.529498 8.4:-27125723 MS.gene28571:CDS
ATAGTATCAGCCCTAGAAGA+AGG 0.536346 8.4:+27125570 None:intergenic
CCCCGATGGTGATGATGGGT+TGG 0.540092 8.4:-27125796 MS.gene28571:CDS
GGTCCAGCAAATGACCTTCA+AGG 0.542129 8.4:-27126062 MS.gene28571:CDS
TGTGTCGTCAACTCAGCAGC+TGG 0.550434 8.4:+27125864 None:intergenic
GATGATTGCACGCTGCGCAC+GGG 0.552985 8.4:+27126294 None:intergenic
ATAGACTGGGTCCCTCAACC+TGG 0.566639 8.4:+27126094 None:intergenic
ATCATTGGTGTATGAGGCTG+AGG 0.566661 8.4:-27126117 MS.gene28571:CDS
CAAGAGTGTGAAGAACGAAC+TGG 0.569336 8.4:-27126012 MS.gene28571:CDS
AAAACGGTGGCACTAGTACT+AGG 0.572087 8.4:-27125740 MS.gene28571:CDS
TGGTGTATGAGGCTGAGGCC+AGG 0.580144 8.4:-27126112 MS.gene28571:CDS
TCTTGTTCGTGATTCTGGGT+TGG 0.583224 8.4:-27125646 MS.gene28571:CDS
CAACCCAGAGCGTTTCATCA+AGG 0.586110 8.4:-27126219 MS.gene28571:CDS
AAGCTTCTTAGTGGCCCCGA+TGG 0.587409 8.4:-27125810 MS.gene28571:CDS
TGACTCGGCAAGATTCCAGA+TGG 0.587492 8.4:-27125679 MS.gene28571:CDS
TGTCAATGGCAGTAGTGACT+CGG 0.588601 8.4:-27125694 MS.gene28571:CDS
AGTACTAGGTCCCACCCTGT+TGG 0.592718 8.4:-27125726 MS.gene28571:CDS
AGCTTGAGTCGCACTCCTGA+CGG 0.594185 8.4:+27126270 None:intergenic
AAACGCTCTGGGTTGTCAGG+TGG 0.594885 8.4:+27126227 None:intergenic
GACCTGCACAGCCATAGACT+GGG 0.612820 8.4:+27126081 None:intergenic
GACTCGGCAAGATTCCAGAT+GGG 0.616618 8.4:-27125678 MS.gene28571:CDS
CTTGCACCGACTGTCATAGT+CGG 0.618697 8.4:+27125909 None:intergenic
TAGGTTGAATGAAGTTCGAA+CGG 0.624296 8.4:-27126039 MS.gene28571:CDS
AAAGCTGAAACAATCAGGAA+AGG 0.628624 8.4:+27125950 None:intergenic
CAGCAAATGACCTTCAAGGT+AGG 0.629800 8.4:-27126058 MS.gene28571:CDS
TGGAACGTCAGGAAGCATCT+GGG 0.630575 8.4:+27125617 None:intergenic
GTGGCCCCGATGGTGATGAT+GGG 0.639305 8.4:-27125800 MS.gene28571:CDS
GTACCTTGATGAAACGCTCT+GGG 0.642313 8.4:+27126216 None:intergenic
ATAACCACCACCAACAGGGT+GGG 0.656525 8.4:+27125716 None:intergenic
AGAACAAATTCAACGACCTG+AGG 0.670458 8.4:+27125975 None:intergenic
TGGGTTGGCAGGATTCCAGA+TGG 0.675884 8.4:-27125781 MS.gene28571:CDS
CATAACCACCACCAACAGGG+TGG 0.680151 8.4:+27125715 None:intergenic
TGACATAACCACCACCAACA+GGG 0.681886 8.4:+27125712 None:intergenic
AGGGCTGATACTATTGACAA+TGG 0.685373 8.4:-27125561 MS.gene28571:CDS
CAGGTGGAAAGTAAGGAGCA+AGG 0.685400 8.4:+27126243 None:intergenic
ACAGAAGGAGCATCAAGATG+TGG 0.689873 8.4:+27125597 None:intergenic
TTTGCACCGACTATGACAGT+CGG 0.698771 8.4:-27125915 MS.gene28571:CDS
ATTCAACGACCTGAGGACTG+AGG 0.713865 8.4:+27125982 None:intergenic
AATGTTCCTGTTGAAAACGG+TGG 0.735325 8.4:-27125753 MS.gene28571:CDS
CCAACCCATCATCACCATCG+GGG 0.738435 8.4:+27125796 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAAAAAAAAATATAGACAA+TGG - chr8.4:27125553-27125572 MS.gene28571:CDS 15.0%
! TGAACAAGTTATTGAATCAT+TGG - chr8.4:27125836-27125855 MS.gene28571:CDS 25.0%
! TTAAAACAAAAGCTTCTTAG+TGG - chr8.4:27126149-27126168 MS.gene28571:CDS 25.0%
GTTAATGTTCCTGTTGAAAA+CGG - chr8.4:27126212-27126231 MS.gene28571:CDS 30.0%
GTTAATTTAATGTGCAGTGA+TGG - chr8.4:27125635-27125654 MS.gene28571:CDS 30.0%
TATTGAATCATTGGTGTATG+AGG - chr8.4:27125845-27125864 MS.gene28571:CDS 30.0%
AAAGCTGAAACAATCAGGAA+AGG + chr8.4:27126021-27126040 None:intergenic 35.0%
AATGTTCTTGTTCGTGATTC+TGG - chr8.4:27126317-27126336 MS.gene28571:CDS 35.0%
ATGTTCTTGTTCGTGATTCT+GGG - chr8.4:27126318-27126337 MS.gene28571:CDS 35.0%
! TAGGTTGAATGAAGTTCGAA+CGG - chr8.4:27125929-27125948 MS.gene28571:CDS 35.0%
AATGTTCCTGTTGAAAACGG+TGG - chr8.4:27126215-27126234 MS.gene28571:CDS 40.0%
AGAACAAATTCAACGACCTG+AGG + chr8.4:27125996-27126015 None:intergenic 40.0%
AGGGCTGATACTATTGACAA+TGG - chr8.4:27126407-27126426 MS.gene28571:intron 40.0%
ATAGTATCAGCCCTAGAAGA+AGG + chr8.4:27126401-27126420 None:intergenic 40.0%
ATAGTCGGTGCAAATGCATT+TGG + chr8.4:27126047-27126066 None:intergenic 40.0%
ATCAAGGTACACTCTGTGTT+TGG - chr8.4:27125765-27125784 MS.gene28571:CDS 40.0%
CAGGAACATTAACATCCATC+TGG + chr8.4:27126205-27126224 None:intergenic 40.0%
CTTCATTCAACCTACCTTGA+AGG + chr8.4:27125923-27125942 None:intergenic 40.0%
GGCTTAAAGCTGAAACAATC+AGG + chr8.4:27126026-27126045 None:intergenic 40.0%
TTCTGTGCTTCCTTCTTCTA+GGG - chr8.4:27126388-27126407 MS.gene28571:intron 40.0%
AAAACGGTGGCACTAGTACT+AGG - chr8.4:27126228-27126247 MS.gene28571:CDS 45.0%
ATCATTGGTGTATGAGGCTG+AGG - chr8.4:27125851-27125870 MS.gene28571:CDS 45.0%
CAAGAACATTAGCACCCATC+TGG + chr8.4:27126307-27126326 None:intergenic 45.0%
CAGCAAATGACCTTCAAGGT+AGG - chr8.4:27125910-27125929 MS.gene28571:CDS 45.0%
CTACCTTGAAGGTCATTTGC+TGG + chr8.4:27125912-27125931 None:intergenic 45.0%
CTTCTGTGCTTCCTTCTTCT+AGG - chr8.4:27126387-27126406 MS.gene28571:intron 45.0%
GTACCTTGATGAAACGCTCT+GGG + chr8.4:27125755-27125774 None:intergenic 45.0%
TCTTGTTCGTGATTCTGGGT+TGG - chr8.4:27126322-27126341 MS.gene28571:intron 45.0%
TGACATAACCACCACCAACA+GGG + chr8.4:27126259-27126278 None:intergenic 45.0%
TGTACCTTGATGAAACGCTC+TGG + chr8.4:27125756-27125775 None:intergenic 45.0%
TGTCAATGGCAGTAGTGACT+CGG - chr8.4:27126274-27126293 MS.gene28571:CDS 45.0%
TTGACATAACCACCACCAAC+AGG + chr8.4:27126260-27126279 None:intergenic 45.0%
! CAAGAGTGTGAAGAACGAAC+TGG - chr8.4:27125956-27125975 MS.gene28571:CDS 45.0%
! CTAGAAGAAGGAAGCACAGA+AGG + chr8.4:27126389-27126408 None:intergenic 45.0%
!! ACAGAAGGAGCATCAAGATG+TGG + chr8.4:27126374-27126393 None:intergenic 45.0%
!! GTTGGTGGTGGTTATGTCAA+TGG - chr8.4:27126260-27126279 MS.gene28571:CDS 45.0%
!! TTTGCACCGACTATGACAGT+CGG - chr8.4:27126053-27126072 MS.gene28571:CDS 45.0%
ATAACCACCACCAACAGGGT+GGG + chr8.4:27126255-27126274 None:intergenic 50.0%
ATTCAACGACCTGAGGACTG+AGG + chr8.4:27125989-27126008 None:intergenic 50.0%
CAGGTGGAAAGTAAGGAGCA+AGG + chr8.4:27125728-27125747 None:intergenic 50.0%
GACAGTCGGTGCAAGACAAT+TGG - chr8.4:27126067-27126086 MS.gene28571:CDS 50.0%
GACTCGGCAAGATTCCAGAT+GGG - chr8.4:27126290-27126309 MS.gene28571:CDS 50.0%
GCATCAAGATGTGGAACGTC+AGG + chr8.4:27126365-27126384 None:intergenic 50.0%
GGGTTGTCAGGTGGAAAGTA+AGG + chr8.4:27125735-27125754 None:intergenic 50.0%
GGTCCAGCAAATGACCTTCA+AGG - chr8.4:27125906-27125925 MS.gene28571:CDS 50.0%
TGCCAACCCATCATCACCAT+CGG + chr8.4:27126177-27126196 None:intergenic 50.0%
! ATGAAACGCTCTGGGTTGTC+AGG + chr8.4:27125747-27125766 None:intergenic 50.0%
! CAACCCAGAGCGTTTCATCA+AGG - chr8.4:27125749-27125768 MS.gene28571:CDS 50.0%
! CTAGTGCCACCGTTTTCAAC+AGG + chr8.4:27126224-27126243 None:intergenic 50.0%
! GACCTGAGGACTGAGGTATT+TGG + chr8.4:27125982-27126001 None:intergenic 50.0%
! TGACTCGGCAAGATTCCAGA+TGG - chr8.4:27126289-27126308 MS.gene28571:CDS 50.0%
!! CTTGCACCGACTGTCATAGT+CGG + chr8.4:27126062-27126081 None:intergenic 50.0%
!! TGGAACGTCAGGAAGCATCT+GGG + chr8.4:27126354-27126373 None:intergenic 50.0%
AAGCTTCTTAGTGGCCCCGA+TGG - chr8.4:27126158-27126177 MS.gene28571:CDS 55.0%
AGATGATTGCACGCTGCGCA+CGG + chr8.4:27125678-27125697 None:intergenic 55.0%
AGCGTGCAATCATCTCCGTC+AGG - chr8.4:27125683-27125702 MS.gene28571:CDS 55.0%
AGCTTGAGTCGCACTCCTGA+CGG + chr8.4:27125701-27125720 None:intergenic 55.0%
AGGTTGAGGGACCCAGTCTA+TGG - chr8.4:27125876-27125895 MS.gene28571:CDS 55.0%
AGTACTAGGTCCCACCCTGT+TGG - chr8.4:27126242-27126261 MS.gene28571:CDS 55.0%
ATAGACTGGGTCCCTCAACC+TGG + chr8.4:27125877-27125896 None:intergenic 55.0%
CATAACCACCACCAACAGGG+TGG + chr8.4:27126256-27126275 None:intergenic 55.0%
CCAACCCATCATCACCATCG+GGG + chr8.4:27126175-27126194 None:intergenic 55.0%
GACCTGCACAGCCATAGACT+GGG + chr8.4:27125890-27125909 None:intergenic 55.0%
GCCAACCCATCATCACCATC+GGG + chr8.4:27126176-27126195 None:intergenic 55.0%
GGCCAAATACCTCAGTCCTC+AGG - chr8.4:27125977-27125996 MS.gene28571:CDS 55.0%
TGTGTCGTCAACTCAGCAGC+TGG + chr8.4:27126107-27126126 None:intergenic 55.0%
! AAACGCTCTGGGTTGTCAGG+TGG + chr8.4:27125744-27125763 None:intergenic 55.0%
!! GATGGTGATGATGGGTTGGC+AGG - chr8.4:27126176-27126195 MS.gene28571:CDS 55.0%
!! GTGGAACGTCAGGAAGCATC+TGG + chr8.4:27126355-27126374 None:intergenic 55.0%
!! GTTCGTGATTCTGGGTTGGC+AGG - chr8.4:27126326-27126345 MS.gene28571:intron 55.0%
!! TGGGTTGGCAGGATTCCAGA+TGG - chr8.4:27126187-27126206 MS.gene28571:CDS 55.0%
ACTAGGTCCCACCCTGTTGG+TGG - chr8.4:27126245-27126264 MS.gene28571:CDS 60.0%
ATGAGGCTGAGGCCAGGTTG+AGG - chr8.4:27125862-27125881 MS.gene28571:CDS 60.0%
GACCCAGTCTATGGCTGTGC+AGG - chr8.4:27125885-27125904 MS.gene28571:CDS 60.0%
GATGATTGCACGCTGCGCAC+GGG + chr8.4:27125677-27125696 None:intergenic 60.0%
GGACCTGCACAGCCATAGAC+TGG + chr8.4:27125891-27125910 None:intergenic 60.0%
TGGTGTATGAGGCTGAGGCC+AGG - chr8.4:27125856-27125875 MS.gene28571:CDS 60.0%
! AGTGGCCCCGATGGTGATGA+TGG - chr8.4:27126167-27126186 MS.gene28571:CDS 60.0%
! GTGGCCCCGATGGTGATGAT+GGG - chr8.4:27126168-27126187 MS.gene28571:CDS 60.0%
! TGAGGCTGAGGCCAGGTTGA+GGG - chr8.4:27125863-27125882 MS.gene28571:CDS 60.0%
!! CCCCGATGGTGATGATGGGT+TGG - chr8.4:27126172-27126191 MS.gene28571:CDS 60.0%
! AGGTCCCACCCTGTTGGTGG+TGG - chr8.4:27126248-27126267 MS.gene28571:CDS 65.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 27125536 27126454 27125536 ID=MS.gene28571
chr8.4 mRNA 27125536 27126454 27125536 ID=MS.gene28571.t1;Parent=MS.gene28571
chr8.4 exon 27126442 27126454 27126442 ID=MS.gene28571.t1.exon1;Parent=MS.gene28571.t1
chr8.4 CDS 27126442 27126454 27126442 ID=cds.MS.gene28571.t1;Parent=MS.gene28571.t1
chr8.4 exon 27125536 27126338 27125536 ID=MS.gene28571.t1.exon2;Parent=MS.gene28571.t1
chr8.4 CDS 27125536 27126338 27125536 ID=cds.MS.gene28571.t1;Parent=MS.gene28571.t1
Gene Sequence

>MS.gene28571

ATGGCATTGTTAGGTCAAGAAAAAAAAATATAGACAATGGATTTCGTTTTCACTTTTTGTGTTATATATTGTTGTTGAATGAACTTAGTAATTGAATTTGTTAATTTAATGTGCAGTGATGGCAAGAGTGATTATTCGCCCGTGCGCAGCGTGCAATCATCTCCGTCAGGAGTGCGACTCAAGCTGTGACCTTGCTCCTTACTTTCCACCTGACAACCCAGAGCGTTTCATCAAGGTACACTCTGTGTTTGGAAAGAACAATGTGTCGAATATGTTGAAAAAAATTGATGCTTCACATCATGAACAAGTTATTGAATCATTGGTGTATGAGGCTGAGGCCAGGTTGAGGGACCCAGTCTATGGCTGTGCAGGTCCAGCAAATGACCTTCAAGGTAGGTTGAATGAAGTTCGAACGGAACTCAAGAGTGTGAAGAACGAACTGGCCAAATACCTCAGTCCTCAGGTCGTTGAATTTGTTCTCGCCTTTCCTGATTGTTTCAGCTTTAAGCCAAATGCATTTGCACCGACTATGACAGTCGGTGCAAGACAATTGGCTTTTAATCAGCTGCCAGCTGCTGAGTTGACGACACAACAGATTCACAACTTAGAAGTGTTAAAACAAAAGCTTCTTAGTGGCCCCGATGGTGATGATGGGTTGGCAGGATTCCAGATGGATGTTAATGTTCCTGTTGAAAACGGTGGCACTAGTACTAGGTCCCACCCTGTTGGTGGTGGTTATGTCAATGGCAGTAGTGACTCGGCAAGATTCCAGATGGGTGCTAATGTTCTTGTTCGTGATTCTGGGTTGGCAGGATCCCAGATGCTTCCTGACGTTCCACATCTTGATGCTCCTTCTGTGCTTCCTTCTTCTAGGGCTGATACTATTGACAATGGTGACACTAGCAACCAACAAGAATAA
Protein sequence

>MS.gene28571.t1

MALLVMARVIIRPCAACNHLRQECDSSCDLAPYFPPDNPERFIKVHSVFGKNNVSNMLKKIDASHHEQVIESLVYEAEARLRDPVYGCAGPANDLQGRLNEVRTELKSVKNELAKYLSPQVVEFVLAFPDCFSFKPNAFAPTMTVGARQLAFNQLPAAELTTQQIHNLEVLKQKLLSGPDGDDGLAGFQMDVNVPVENGGTSTRSHPVGGGYVNGSSDSARFQMGANVLVRDSGLAGSQMLPDVPHLDAPSVLPSSRADTIDNGDTSNQQE