Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28574.t1 | XP_003629569.1 | 86.4 | 228 | 22 | 2 | 1 | 219 | 1 | 228 | 1.10E-101 | 379.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28574.t1 | Q9FKZ3 | 49.6 | 127 | 62 | 2 | 8 | 134 | 7 | 131 | 1.7e-26 | 120.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28574.t1 | G7LGC1 | 86.4 | 228 | 22 | 2 | 1 | 219 | 1 | 228 | 8.1e-102 | 379.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene28574.t1 | TF | LOB |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28574.t1 | MTR_8g079580 | 80.383 | 209 | 32 | 1 | 1 | 200 | 1 | 209 | 9.50e-118 | 334 |
| MS.gene28574.t1 | MTR_8g079660 | 52.174 | 184 | 82 | 3 | 1 | 180 | 1 | 182 | 2.26e-57 | 182 |
| MS.gene28574.t1 | MTR_8g079620 | 45.894 | 207 | 103 | 4 | 1 | 202 | 1 | 203 | 9.76e-54 | 172 |
| MS.gene28574.t1 | MTR_3g073690 | 57.658 | 111 | 46 | 1 | 8 | 118 | 9 | 118 | 3.86e-39 | 134 |
| MS.gene28574.t1 | MTR_5g017950 | 52.066 | 121 | 57 | 1 | 1 | 121 | 1 | 120 | 5.81e-37 | 131 |
| MS.gene28574.t1 | MTR_3g452660 | 44.172 | 163 | 66 | 4 | 8 | 146 | 7 | 168 | 1.52e-35 | 127 |
| MS.gene28574.t1 | MTR_3g031660 | 50.000 | 102 | 50 | 1 | 8 | 109 | 7 | 107 | 2.31e-32 | 115 |
| MS.gene28574.t1 | MTR_5g083230 | 50.980 | 102 | 49 | 1 | 8 | 109 | 6 | 106 | 6.81e-32 | 114 |
| MS.gene28574.t1 | MTR_4g105170 | 50.476 | 105 | 51 | 1 | 8 | 112 | 11 | 114 | 1.39e-30 | 111 |
| MS.gene28574.t1 | MTR_3g071590 | 47.706 | 109 | 56 | 1 | 1 | 109 | 1 | 108 | 2.16e-30 | 110 |
| MS.gene28574.t1 | MTR_2g093310 | 49.524 | 105 | 52 | 1 | 5 | 109 | 30 | 133 | 8.94e-30 | 109 |
| MS.gene28574.t1 | MTR_3g077240 | 48.182 | 110 | 56 | 1 | 8 | 117 | 10 | 118 | 1.55e-29 | 108 |
| MS.gene28574.t1 | MTR_7g033800 | 49.524 | 105 | 52 | 1 | 8 | 112 | 11 | 114 | 2.21e-29 | 108 |
| MS.gene28574.t1 | MTR_3g094690 | 50.467 | 107 | 52 | 1 | 6 | 112 | 45 | 150 | 4.13e-29 | 109 |
| MS.gene28574.t1 | MTR_4g060950 | 46.667 | 105 | 56 | 0 | 5 | 109 | 21 | 125 | 4.73e-29 | 107 |
| MS.gene28574.t1 | MTR_5g083960 | 50.476 | 105 | 51 | 1 | 8 | 112 | 39 | 142 | 6.90e-29 | 107 |
| MS.gene28574.t1 | MTR_2g068760 | 48.571 | 105 | 53 | 1 | 6 | 110 | 11 | 114 | 9.86e-29 | 105 |
| MS.gene28574.t1 | MTR_6g005070 | 48.077 | 104 | 53 | 1 | 8 | 111 | 9 | 111 | 4.79e-28 | 103 |
| MS.gene28574.t1 | MTR_3g094690 | 50.467 | 107 | 51 | 2 | 6 | 112 | 45 | 149 | 5.90e-28 | 106 |
| MS.gene28574.t1 | MTR_5g075020 | 52.336 | 107 | 50 | 1 | 6 | 112 | 41 | 146 | 1.20e-27 | 105 |
| MS.gene28574.t1 | MTR_5g075020 | 52.336 | 107 | 50 | 1 | 6 | 112 | 45 | 150 | 1.21e-27 | 105 |
| MS.gene28574.t1 | MTR_4g083680 | 50.495 | 101 | 49 | 1 | 8 | 108 | 15 | 114 | 1.29e-27 | 103 |
| MS.gene28574.t1 | MTR_7g028905 | 45.370 | 108 | 58 | 1 | 5 | 112 | 2 | 108 | 2.95e-27 | 100 |
| MS.gene28574.t1 | MTR_6g005080 | 46.667 | 105 | 55 | 1 | 8 | 112 | 10 | 113 | 3.01e-27 | 101 |
| MS.gene28574.t1 | MTR_5g093010 | 46.078 | 102 | 54 | 1 | 9 | 110 | 6 | 106 | 3.56e-27 | 101 |
| MS.gene28574.t1 | MTR_1g095850 | 49.020 | 102 | 51 | 1 | 9 | 110 | 73 | 173 | 5.83e-27 | 103 |
| MS.gene28574.t1 | MTR_8g040900 | 49.091 | 110 | 55 | 1 | 8 | 117 | 7 | 115 | 4.99e-26 | 100 |
| MS.gene28574.t1 | MTR_5g083010 | 45.631 | 103 | 55 | 1 | 9 | 111 | 12 | 113 | 1.19e-24 | 95.9 |
| MS.gene28574.t1 | MTR_3g071420 | 47.959 | 98 | 50 | 1 | 9 | 106 | 15 | 111 | 4.56e-24 | 94.7 |
| MS.gene28574.t1 | MTR_6g027710 | 43.519 | 108 | 59 | 2 | 3 | 109 | 35 | 141 | 4.15e-23 | 93.2 |
| MS.gene28574.t1 | MTR_4g099230 | 43.689 | 103 | 56 | 2 | 9 | 110 | 3 | 104 | 1.91e-21 | 87.0 |
| MS.gene28574.t1 | MTR_7g096530 | 41.667 | 108 | 61 | 2 | 8 | 114 | 14 | 120 | 5.86e-21 | 87.4 |
| MS.gene28574.t1 | MTR_7g074990 | 43.689 | 103 | 56 | 2 | 8 | 109 | 20 | 121 | 1.88e-20 | 86.3 |
| MS.gene28574.t1 | MTR_8g036085 | 41.509 | 106 | 60 | 2 | 8 | 112 | 23 | 127 | 2.77e-20 | 85.9 |
| MS.gene28574.t1 | MTR_1g070220 | 35.862 | 145 | 74 | 5 | 8 | 138 | 15 | 154 | 9.63e-20 | 84.0 |
| MS.gene28574.t1 | MTR_6g011250 | 40.777 | 103 | 59 | 2 | 9 | 110 | 10 | 111 | 1.19e-18 | 80.5 |
| MS.gene28574.t1 | MTR_6g027700 | 39.423 | 104 | 61 | 2 | 4 | 106 | 3 | 105 | 1.82e-18 | 80.5 |
| MS.gene28574.t1 | MTR_1g070205 | 42.000 | 100 | 56 | 2 | 8 | 106 | 12 | 110 | 2.03e-18 | 80.9 |
| MS.gene28574.t1 | MTR_7g075230 | 40.000 | 105 | 61 | 1 | 8 | 110 | 12 | 116 | 4.10e-18 | 79.3 |
| MS.gene28574.t1 | MTR_8g036105 | 43.269 | 104 | 57 | 2 | 8 | 110 | 18 | 120 | 1.08e-17 | 78.6 |
| MS.gene28574.t1 | MTR_7g096610 | 32.903 | 155 | 87 | 5 | 8 | 158 | 8 | 149 | 1.43e-17 | 78.6 |
| MS.gene28574.t1 | MTR_6g011230 | 34.351 | 131 | 82 | 2 | 7 | 134 | 10 | 139 | 1.66e-17 | 77.8 |
| MS.gene28574.t1 | MTR_5g085390 | 40.909 | 110 | 60 | 3 | 5 | 110 | 3 | 111 | 2.92e-17 | 77.8 |
| MS.gene28574.t1 | MTR_6g011200 | 38.679 | 106 | 60 | 3 | 5 | 106 | 3 | 107 | 3.32e-17 | 77.8 |
| MS.gene28574.t1 | MTR_5g080470 | 40.000 | 95 | 55 | 2 | 9 | 102 | 8 | 101 | 4.06e-17 | 76.6 |
| MS.gene28574.t1 | MTR_4g107450 | 39.806 | 103 | 61 | 1 | 7 | 109 | 9 | 110 | 4.09e-17 | 78.6 |
| MS.gene28574.t1 | MTR_2g100020 | 34.314 | 102 | 66 | 1 | 9 | 110 | 20 | 120 | 6.73e-14 | 69.3 |
| MS.gene28574.t1 | MTR_8g017090 | 35.644 | 101 | 57 | 2 | 9 | 109 | 13 | 105 | 1.03e-13 | 68.9 |
| MS.gene28574.t1 | MTR_4g088035 | 37.975 | 79 | 48 | 1 | 9 | 87 | 7 | 84 | 1.18e-12 | 65.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28574.t1 | AT5G66870 | 49.606 | 127 | 62 | 2 | 8 | 134 | 7 | 131 | 7.08e-35 | 126 |
| MS.gene28574.t1 | AT2G23660 | 51.351 | 111 | 53 | 1 | 8 | 118 | 5 | 114 | 3.15e-34 | 124 |
| MS.gene28574.t1 | AT2G23660 | 51.351 | 111 | 53 | 1 | 8 | 118 | 5 | 114 | 3.15e-34 | 124 |
| MS.gene28574.t1 | AT2G23660 | 51.351 | 111 | 53 | 1 | 8 | 118 | 5 | 114 | 3.15e-34 | 124 |
| MS.gene28574.t1 | AT1G16530 | 50.000 | 104 | 52 | 0 | 6 | 109 | 12 | 115 | 5.16e-32 | 114 |
| MS.gene28574.t1 | AT2G30130 | 50.000 | 102 | 50 | 1 | 8 | 109 | 8 | 108 | 2.05e-30 | 111 |
| MS.gene28574.t1 | AT1G07900 | 48.113 | 106 | 54 | 1 | 5 | 110 | 30 | 134 | 6.57e-30 | 110 |
| MS.gene28574.t1 | AT5G63090 | 48.571 | 105 | 53 | 1 | 8 | 112 | 11 | 114 | 2.85e-29 | 108 |
| MS.gene28574.t1 | AT5G63090 | 48.571 | 105 | 53 | 1 | 8 | 112 | 11 | 114 | 2.85e-29 | 108 |
| MS.gene28574.t1 | AT5G63090 | 48.571 | 105 | 53 | 1 | 8 | 112 | 11 | 114 | 2.85e-29 | 108 |
| MS.gene28574.t1 | AT5G63090 | 48.571 | 105 | 53 | 1 | 8 | 112 | 11 | 114 | 2.85e-29 | 108 |
| MS.gene28574.t1 | AT3G27650 | 48.571 | 105 | 53 | 1 | 8 | 112 | 39 | 142 | 3.50e-29 | 107 |
| MS.gene28574.t1 | AT2G28500 | 47.170 | 106 | 55 | 1 | 5 | 110 | 52 | 156 | 4.71e-29 | 108 |
| MS.gene28574.t1 | AT2G40470 | 47.244 | 127 | 64 | 2 | 6 | 130 | 18 | 143 | 7.71e-29 | 107 |
| MS.gene28574.t1 | AT2G30340 | 48.598 | 107 | 54 | 1 | 6 | 112 | 51 | 156 | 9.54e-29 | 108 |
| MS.gene28574.t1 | AT2G30340 | 48.598 | 107 | 54 | 1 | 6 | 112 | 50 | 155 | 1.04e-28 | 108 |
| MS.gene28574.t1 | AT2G40470 | 47.244 | 127 | 64 | 2 | 6 | 130 | 43 | 168 | 1.97e-28 | 107 |
| MS.gene28574.t1 | AT1G65620 | 54.286 | 105 | 47 | 1 | 8 | 112 | 9 | 112 | 5.79e-28 | 105 |
| MS.gene28574.t1 | AT1G65620 | 54.286 | 105 | 47 | 1 | 8 | 112 | 9 | 112 | 5.79e-28 | 105 |
| MS.gene28574.t1 | AT1G65620 | 54.286 | 105 | 47 | 1 | 8 | 112 | 9 | 112 | 5.79e-28 | 105 |
| MS.gene28574.t1 | AT1G65620 | 54.286 | 105 | 47 | 1 | 8 | 112 | 9 | 112 | 5.79e-28 | 105 |
| MS.gene28574.t1 | AT1G65620 | 54.286 | 105 | 47 | 1 | 8 | 112 | 9 | 112 | 5.79e-28 | 105 |
| MS.gene28574.t1 | AT1G31320 | 56.818 | 88 | 37 | 1 | 8 | 95 | 13 | 99 | 7.21e-28 | 104 |
| MS.gene28574.t1 | AT3G26660 | 43.137 | 102 | 57 | 1 | 9 | 110 | 6 | 106 | 1.56e-24 | 94.0 |
| MS.gene28574.t1 | AT3G26620 | 43.137 | 102 | 57 | 1 | 9 | 110 | 6 | 106 | 1.70e-24 | 94.0 |
| MS.gene28574.t1 | AT3G11090 | 45.192 | 104 | 56 | 1 | 9 | 112 | 12 | 114 | 1.20e-23 | 93.2 |
| MS.gene28574.t1 | AT3G50510 | 40.909 | 110 | 64 | 1 | 3 | 112 | 7 | 115 | 2.14e-22 | 90.9 |
| MS.gene28574.t1 | AT3G50510 | 40.909 | 110 | 64 | 1 | 3 | 112 | 7 | 115 | 2.14e-22 | 90.9 |
| MS.gene28574.t1 | AT4G00210 | 45.192 | 104 | 55 | 2 | 8 | 110 | 11 | 113 | 1.23e-20 | 86.7 |
| MS.gene28574.t1 | AT3G03760 | 43.689 | 103 | 56 | 2 | 8 | 109 | 51 | 152 | 2.05e-20 | 87.0 |
| MS.gene28574.t1 | AT3G47870 | 39.423 | 104 | 62 | 1 | 9 | 112 | 37 | 139 | 4.74e-20 | 87.0 |
| MS.gene28574.t1 | AT2G42430 | 39.623 | 106 | 62 | 2 | 8 | 112 | 15 | 119 | 2.46e-19 | 83.6 |
| MS.gene28574.t1 | AT3G58190 | 42.157 | 102 | 57 | 2 | 8 | 108 | 11 | 111 | 5.16e-19 | 82.4 |
| MS.gene28574.t1 | AT2G42440 | 36.066 | 122 | 75 | 3 | 8 | 128 | 7 | 126 | 1.58e-18 | 81.6 |
| MS.gene28574.t1 | AT2G45410 | 39.437 | 142 | 62 | 5 | 11 | 151 | 19 | 137 | 3.80e-18 | 79.3 |
| MS.gene28574.t1 | AT5G35900 | 27.805 | 205 | 120 | 5 | 9 | 201 | 6 | 194 | 2.61e-17 | 77.4 |
| MS.gene28574.t1 | AT4G00220 | 40.594 | 101 | 58 | 2 | 11 | 110 | 20 | 119 | 1.87e-16 | 75.5 |
| MS.gene28574.t1 | AT2G45420 | 38.235 | 102 | 61 | 2 | 11 | 111 | 40 | 140 | 9.07e-16 | 74.3 |
| MS.gene28574.t1 | AT3G13850 | 33.962 | 106 | 69 | 1 | 9 | 114 | 37 | 141 | 2.81e-15 | 73.2 |
| MS.gene28574.t1 | AT1G06280 | 40.449 | 89 | 52 | 1 | 8 | 96 | 24 | 111 | 6.94e-15 | 70.9 |
| MS.gene28574.t1 | AT5G06080 | 35.849 | 106 | 66 | 2 | 8 | 112 | 7 | 111 | 9.92e-15 | 69.7 |
| MS.gene28574.t1 | AT1G72980 | 36.275 | 102 | 64 | 1 | 8 | 109 | 13 | 113 | 4.21e-14 | 68.9 |
| MS.gene28574.t1 | AT2G31310 | 34.286 | 105 | 67 | 2 | 8 | 111 | 7 | 110 | 1.05e-13 | 67.4 |
Find 53 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGTTTGAGGGACAATGTTTA+TGG | 0.203988 | 8.4:-27090219 | MS.gene28574:CDS |
| CAAACAAGTCGCTGATTCTT+TGG | 0.280466 | 8.4:-27090259 | MS.gene28574:CDS |
| TTCAACTATTCTTGTTGATC+TGG | 0.304345 | 8.4:+27089802 | None:intergenic |
| CTGTCCAATCCTGATCGTTT+CGG | 0.322703 | 8.4:-27090081 | MS.gene28574:CDS |
| TATTGAAAGAAAAGATTGTT+AGG | 0.385848 | 8.4:-27089957 | MS.gene28574:CDS |
| AACCTCGTACACTTAGTGTT+TGG | 0.411671 | 8.4:-27090330 | MS.gene28574:CDS |
| CTTTCTTGAAAAGACATTGA+AGG | 0.413713 | 8.4:-27090178 | MS.gene28574:CDS |
| CGAAGCTGAGGCACGTTTGA+GGG | 0.455458 | 8.4:-27090232 | MS.gene28574:CDS |
| GGTGGAGAGGGACAACATAT+TGG | 0.461387 | 8.4:-27089904 | MS.gene28574:CDS |
| GGTGCAAAGCCGAAACGATC+AGG | 0.465489 | 8.4:+27090072 | None:intergenic |
| AAACGTTGTGGGTTGTCCGC+TGG | 0.468485 | 8.4:+27090354 | None:intergenic |
| GACAATGTTTATGGCTGTGT+TGG | 0.469619 | 8.4:-27090210 | MS.gene28574:CDS |
| CGGTTGCCAGATTAAAGTGC+AGG | 0.478440 | 8.4:-27089851 | MS.gene28574:CDS |
| ATAGAGTTGATCCCGGTGTC+GGG | 0.478762 | 8.4:+27089934 | None:intergenic |
| TGACGCTTGCATGCTGCACA+CGG | 0.480937 | 8.4:+27090423 | None:intergenic |
| ATCAACTCTATTGGTGGTGG+TGG | 0.484132 | 8.4:-27089922 | MS.gene28574:CDS |
| GGGTTGTCCGCTGGAAAGTA+AGG | 0.487972 | 8.4:+27090363 | None:intergenic |
| AGAACCTCTTGAATGATCTC+AGG | 0.498563 | 8.4:+27090102 | None:intergenic |
| GACACCGGGATCAACTCTAT+TGG | 0.505345 | 8.4:-27089931 | MS.gene28574:CDS |
| CATGCAAGCGTCAACGTCGA+AGG | 0.516172 | 8.4:-27090413 | MS.gene28574:CDS |
| CTCTATTGGTGGTGGTGGAG+AGG | 0.524221 | 8.4:-27089917 | MS.gene28574:CDS |
| AATCAGCGACTTGTTTGCGA+TGG | 0.533026 | 8.4:+27090265 | None:intergenic |
| CTTGAATGATCTCAGGATCA+AGG | 0.539423 | 8.4:+27090109 | None:intergenic |
| TCTATTGGTGGTGGTGGAGA+GGG | 0.541503 | 8.4:-27089916 | MS.gene28574:CDS |
| GTCGTCAACTGATCAAAAGT+TGG | 0.547342 | 8.4:+27090003 | None:intergenic |
| AATAGAGTTGATCCCGGTGT+CGG | 0.551053 | 8.4:+27089933 | None:intergenic |
| ACCGGGATCAACTCTATTGG+TGG | 0.553937 | 8.4:-27089928 | MS.gene28574:CDS |
| CTTGACAATGGCAGCACTAG+CGG | 0.562043 | 8.4:-27089871 | MS.gene28574:CDS |
| ACGAAGCTGAGGCACGTTTG+AGG | 0.564133 | 8.4:-27090233 | MS.gene28574:CDS |
| TGTACGAGGTTGAAACGTTG+TGG | 0.572380 | 8.4:+27090342 | None:intergenic |
| ATCAGCGACTTGTTTGCGAT+GGG | 0.575116 | 8.4:+27090266 | None:intergenic |
| GGTGCTCCTTCTCTTGACAA+TGG | 0.575384 | 8.4:-27089883 | MS.gene28574:CDS |
| TTCTTTGGTGTACGAAGCTG+AGG | 0.576999 | 8.4:-27090244 | MS.gene28574:CDS |
| TGATCCTGAGATCATTCAAG+AGG | 0.583673 | 8.4:-27090106 | MS.gene28574:CDS |
| AAAGCCGAAACGATCAGGAT+TGG | 0.585751 | 8.4:+27090077 | None:intergenic |
| TGTTCAAGATGAACTCAAGA+AGG | 0.590687 | 8.4:-27090154 | MS.gene28574:CDS |
| GGGATCAACTCTATTGGTGG+TGG | 0.590748 | 8.4:-27089925 | MS.gene28574:CDS |
| GTCAACTGATCAAAAGTTGG+TGG | 0.590790 | 8.4:+27090006 | None:intergenic |
| AAGATTGTTAGGCCCGACAC+CGG | 0.593658 | 8.4:-27089946 | MS.gene28574:CDS |
| TCGTACACTTAGTGTTTGGA+AGG | 0.594928 | 8.4:-27090326 | MS.gene28574:CDS |
| GTACGAGGTTGAAACGTTGT+GGG | 0.597027 | 8.4:+27090343 | None:intergenic |
| GTGCTGCCATTGTCAAGAGA+AGG | 0.597898 | 8.4:+27089877 | None:intergenic |
| GGTTGCCAGATTAAAGTGCA+GGG | 0.607171 | 8.4:-27089850 | MS.gene28574:CDS |
| CCGCTGGAAAGTAAGGAGCT+AGG | 0.607576 | 8.4:+27090370 | None:intergenic |
| GACGCTTGCATGCTGCACAC+GGG | 0.614011 | 8.4:+27090424 | None:intergenic |
| AGATTGTTAGGCCCGACACC+GGG | 0.617611 | 8.4:-27089945 | MS.gene28574:CDS |
| ACCACCAATAGAGTTGATCC+CGG | 0.637012 | 8.4:+27089927 | None:intergenic |
| CCTAGCTCCTTACTTTCCAG+CGG | 0.652064 | 8.4:-27090370 | MS.gene28574:CDS |
| TGAGAAACAAGATTTCCTTG+TGG | 0.660079 | 8.4:+27090033 | None:intergenic |
| TTGCCAGATTAAAGTGCAGG+GGG | 0.671609 | 8.4:-27089848 | MS.gene28574:CDS |
| GTTGCCAGATTAAAGTGCAG+GGG | 0.677079 | 8.4:-27089849 | MS.gene28574:CDS |
| AGCCCTAATCAAAATCCACA+AGG | 0.702653 | 8.4:-27090048 | MS.gene28574:CDS |
| TTCCAAACACTAAGTGTACG+AGG | 0.708950 | 8.4:+27090328 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATTGAAAGAAAAGATTGTT+AGG | - | chr8.4:27090294-27090313 | MS.gene28574:CDS | 20.0% |
| CTTTCTTGAAAAGACATTGA+AGG | - | chr8.4:27090073-27090092 | MS.gene28574:CDS | 30.0% | |
| !! | CTTCAATGTCTTTTCAAGAA+AGG | + | chr8.4:27090075-27090094 | None:intergenic | 30.0% |
| !!! | TTCCTTGTGGATTTTGATTA+GGG | + | chr8.4:27090208-27090227 | None:intergenic | 30.0% |
| !!! | TTTCCTTGTGGATTTTGATT+AGG | + | chr8.4:27090209-27090228 | None:intergenic | 30.0% |
| TGAGAAACAAGATTTCCTTG+TGG | + | chr8.4:27090221-27090240 | None:intergenic | 35.0% | |
| TGTTCAAGATGAACTCAAGA+AGG | - | chr8.4:27090097-27090116 | MS.gene28574:CDS | 35.0% | |
| AACCTCGTACACTTAGTGTT+TGG | - | chr8.4:27089921-27089940 | MS.gene28574:CDS | 40.0% | |
| AGAACCTCTTGAATGATCTC+AGG | + | chr8.4:27090152-27090171 | None:intergenic | 40.0% | |
| AGCCCTAATCAAAATCCACA+AGG | - | chr8.4:27090203-27090222 | MS.gene28574:CDS | 40.0% | |
| CGTTTGAGGGACAATGTTTA+TGG | - | chr8.4:27090032-27090051 | MS.gene28574:CDS | 40.0% | |
| CTTGAATGATCTCAGGATCA+AGG | + | chr8.4:27090145-27090164 | None:intergenic | 40.0% | |
| GACAATGTTTATGGCTGTGT+TGG | - | chr8.4:27090041-27090060 | MS.gene28574:CDS | 40.0% | |
| GTCAACTGATCAAAAGTTGG+TGG | + | chr8.4:27090248-27090267 | None:intergenic | 40.0% | |
| GTCGTCAACTGATCAAAAGT+TGG | + | chr8.4:27090251-27090270 | None:intergenic | 40.0% | |
| TCGTACACTTAGTGTTTGGA+AGG | - | chr8.4:27089925-27089944 | MS.gene28574:CDS | 40.0% | |
| TGATCCTGAGATCATTCAAG+AGG | - | chr8.4:27090145-27090164 | MS.gene28574:CDS | 40.0% | |
| TTCCAAACACTAAGTGTACG+AGG | + | chr8.4:27089926-27089945 | None:intergenic | 40.0% | |
| !! | CAAACAAGTCGCTGATTCTT+TGG | - | chr8.4:27089992-27090011 | MS.gene28574:CDS | 40.0% |
| !!! | TGTGGATTTTGATTAGGGCT+TGG | + | chr8.4:27090203-27090222 | None:intergenic | 40.0% |
| AAAGCCGAAACGATCAGGAT+TGG | + | chr8.4:27090177-27090196 | None:intergenic | 45.0% | |
| CTGTCCAATCCTGATCGTTT+CGG | - | chr8.4:27090170-27090189 | MS.gene28574:CDS | 45.0% | |
| GGTTGCCAGATTAAAGTGCA+GGG | - | chr8.4:27090401-27090420 | MS.gene28574:CDS | 45.0% | |
| GTACGAGGTTGAAACGTTGT+GGG | + | chr8.4:27089911-27089930 | None:intergenic | 45.0% | |
| GTTGCCAGATTAAAGTGCAG+GGG | - | chr8.4:27090402-27090421 | MS.gene28574:CDS | 45.0% | |
| TGTACGAGGTTGAAACGTTG+TGG | + | chr8.4:27089912-27089931 | None:intergenic | 45.0% | |
| TTGCCAGATTAAAGTGCAGG+GGG | - | chr8.4:27090403-27090422 | MS.gene28574:CDS | 45.0% | |
| ! | AATAGAGTTGATCCCGGTGT+CGG | + | chr8.4:27090321-27090340 | None:intergenic | 45.0% |
| ! | AATCAGCGACTTGTTTGCGA+TGG | + | chr8.4:27089989-27090008 | None:intergenic | 45.0% |
| ! | ACCACCAATAGAGTTGATCC+CGG | + | chr8.4:27090327-27090346 | None:intergenic | 45.0% |
| ! | ATCAGCGACTTGTTTGCGAT+GGG | + | chr8.4:27089988-27090007 | None:intergenic | 45.0% |
| ! | TTCTTTGGTGTACGAAGCTG+AGG | - | chr8.4:27090007-27090026 | MS.gene28574:CDS | 45.0% |
| !! | ATCAACTCTATTGGTGGTGG+TGG | - | chr8.4:27090329-27090348 | MS.gene28574:CDS | 45.0% |
| AAGATTGTTAGGCCCGACAC+CGG | - | chr8.4:27090305-27090324 | MS.gene28574:CDS | 50.0% | |
| ACCGGGATCAACTCTATTGG+TGG | - | chr8.4:27090323-27090342 | MS.gene28574:CDS | 50.0% | |
| CCTAGCTCCTTACTTTCCAG+CGG | - | chr8.4:27089881-27089900 | MS.gene28574:CDS | 50.0% | |
| CGGTTGCCAGATTAAAGTGC+AGG | - | chr8.4:27090400-27090419 | MS.gene28574:CDS | 50.0% | |
| CTTGACAATGGCAGCACTAG+CGG | - | chr8.4:27090380-27090399 | MS.gene28574:CDS | 50.0% | |
| GACACCGGGATCAACTCTAT+TGG | - | chr8.4:27090320-27090339 | MS.gene28574:CDS | 50.0% | |
| GGGATCAACTCTATTGGTGG+TGG | - | chr8.4:27090326-27090345 | MS.gene28574:CDS | 50.0% | |
| GGTGCTCCTTCTCTTGACAA+TGG | - | chr8.4:27090368-27090387 | MS.gene28574:CDS | 50.0% | |
| GGTGGAGAGGGACAACATAT+TGG | - | chr8.4:27090347-27090366 | MS.gene28574:CDS | 50.0% | |
| GTGCTGCCATTGTCAAGAGA+AGG | + | chr8.4:27090377-27090396 | None:intergenic | 50.0% | |
| GTTCCCCCTGCACTTTAATC+TGG | + | chr8.4:27090409-27090428 | None:intergenic | 50.0% | |
| ! | ATAGAGTTGATCCCGGTGTC+GGG | + | chr8.4:27090320-27090339 | None:intergenic | 50.0% |
| !! | TCTATTGGTGGTGGTGGAGA+GGG | - | chr8.4:27090335-27090354 | MS.gene28574:CDS | 50.0% |
| ACGAAGCTGAGGCACGTTTG+AGG | - | chr8.4:27090018-27090037 | MS.gene28574:CDS | 55.0% | |
| AGATTGTTAGGCCCGACACC+GGG | - | chr8.4:27090306-27090325 | MS.gene28574:CDS | 55.0% | |
| CATGCAAGCGTCAACGTCGA+AGG | - | chr8.4:27089838-27089857 | MS.gene28574:CDS | 55.0% | |
| CCGCTGGAAAGTAAGGAGCT+AGG | + | chr8.4:27089884-27089903 | None:intergenic | 55.0% | |
| CGAAGCTGAGGCACGTTTGA+GGG | - | chr8.4:27090019-27090038 | MS.gene28574:CDS | 55.0% | |
| GGGTTGTCCGCTGGAAAGTA+AGG | + | chr8.4:27089891-27089910 | None:intergenic | 55.0% | |
| GGTGCAAAGCCGAAACGATC+AGG | + | chr8.4:27090182-27090201 | None:intergenic | 55.0% | |
| TGACGCTTGCATGCTGCACA+CGG | + | chr8.4:27089831-27089850 | None:intergenic | 55.0% | |
| ! | AAACGTTGTGGGTTGTCCGC+TGG | + | chr8.4:27089900-27089919 | None:intergenic | 55.0% |
| !! | CTCTATTGGTGGTGGTGGAG+AGG | - | chr8.4:27090334-27090353 | MS.gene28574:CDS | 55.0% |
| GACGCTTGCATGCTGCACAC+GGG | + | chr8.4:27089830-27089849 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 27089807 | 27090466 | 27089807 | ID=MS.gene28574 |
| chr8.4 | mRNA | 27089807 | 27090466 | 27089807 | ID=MS.gene28574.t1;Parent=MS.gene28574 |
| chr8.4 | exon | 27089807 | 27090466 | 27089807 | ID=MS.gene28574.t1.exon1;Parent=MS.gene28574.t1 |
| chr8.4 | CDS | 27089807 | 27090466 | 27089807 | ID=cds.MS.gene28574.t1;Parent=MS.gene28574.t1 |
| Gene Sequence |
| Protein sequence |
