AlfalfaGEDB | MS.gene28663

M. sativa cultivar XinJiangDaYe / MS.gene28663

KEGG: -
GO: -
PFAM: PF03101.18

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28663.t1	XP_024632821.1	51.9	563	61	9	11	363	1	563	1.60E-133	486.1

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28663.t1	Q9SZL8	32.8	262	164	6	4	254	70	330	7.0e-34	146.4

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28663.t1	A0A2Z6MJF1	69.8	275	75	2	1	267	758	1032	1.3e-105	392.9

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene28663.t1	TF	FAR1

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28663.t1	MTR_4g102720	54.971	171	44	1	1	171	1	138	4.62e-57	186
MS.gene28663.t1	MTR_5g039760	39.175	194	92	3	10	201	13	182	1.60e-40	142
MS.gene28663.t1	MTR_7g074790	41.618	173	82	2	3	174	6	160	4.44e-38	137
MS.gene28663.t1	MTR_0558s0020	32.231	242	143	4	9	229	38	279	2.54e-37	137
MS.gene28663.t1	MTR_7g061980	50.442	113	55	1	4	115	7	119	7.51e-33	120
MS.gene28663.t1	MTR_0402s0040	41.111	180	102	3	7	183	26	204	9.27e-33	129
MS.gene28663.t1	MTR_1g083640	30.870	230	138	4	9	217	53	282	1.47e-31	121
MS.gene28663.t1	MTR_6g051425	29.961	257	168	6	10	254	20	276	5.25e-30	118
MS.gene28663.t1	MTR_8g446020	34.118	170	110	1	62	229	70	239	1.45e-28	112
MS.gene28663.t1	MTR_6g090505	32.821	195	115	3	51	229	20	214	1.18e-27	112

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28663.t1	AT4G38180	32.824	262	164	6	4	254	70	330	1.21e-34	136
MS.gene28663.t1	AT4G15090	27.074	229	163	4	4	229	48	275	2.87e-23	102
MS.gene28663.t1	AT4G15090	27.074	229	163	4	4	229	48	275	2.87e-23	102
MS.gene28663.t1	AT4G15090	27.074	229	163	4	4	229	48	275	2.87e-23	102
MS.gene28663.t1	AT4G15090	27.074	229	163	4	4	229	48	275	2.87e-23	102
MS.gene28663.t1	AT4G15090	27.074	229	163	4	4	229	48	275	2.87e-23	102
MS.gene28663.t1	AT1G76320	28.287	251	161	7	11	252	1	241	8.18e-20	92.0
MS.gene28663.t1	AT1G76320	28.287	251	161	7	11	252	1	241	8.19e-20	92.0
MS.gene28663.t1	AT1G76320	28.287	251	161	7	11	252	1	241	8.19e-20	92.0
MS.gene28663.t1	AT1G76320	28.287	251	161	7	11	252	1	241	1.08e-19	91.7

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Find 86 sgRNAs with CRISPR-Local

Find 137 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGCACAAATGGAGACTT+AGG	0.461223	4.2:+20942947	MS.gene28663:CDS
AAAGTATTGTAACAGATAGT+CGG	0.556314	4.2:-20941872	None:intergenic
AAGGATAGGATTTAAGAATA+TGG	0.268238	4.2:+20941579	MS.gene28663:CDS
AAGGCGTATAAATCCCAAGT+TGG	0.596512	4.2:-20941794	None:intergenic
AAGTTGCTGATGAGTTGGAT+AGG	0.645499	4.2:+20942592	MS.gene28663:CDS
AATCAAATGAATTCCTTTGT+TGG	0.345771	4.2:-20941744	None:intergenic
AATGCTACTTTATGTTCTTT+TGG	0.263418	4.2:+20943131	MS.gene28663:CDS
AATGTCACTTTAAGCTCATT+TGG	0.331866	4.2:+20943161	MS.gene28663:CDS
ACAAACAAACCTGCAAGAAG+TGG	0.580136	4.2:-20941473	None:intergenic
ACCAACTGCAAAGCAAGGAT+AGG	0.623297	4.2:+20941565	MS.gene28663:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAAGAAAGAAGGAAGAAG+AGG	+	chr4.2:20942729-20942748	MS.gene28663:intron	30.0%
AAAAAGAAGGGAAAGAAGTT+TGG	+	chr4.2:20941837-20941856	MS.gene28663:CDS	30.0%
AAACAAAAGAGTCATACAAG+TGG	+	chr4.2:20942136-20942155	MS.gene28663:intron	30.0%
AACATTATATCTTGAAGAGG+TGG	+	chr4.2:20942410-20942429	MS.gene28663:intron	30.0%
AGAATTATCTCCGAAAAAGA+AGG	+	chr4.2:20941824-20941843	MS.gene28663:CDS	30.0%
ATAGGATTTAAGAATATGGG+TGG	+	chr4.2:20941583-20941602	MS.gene28663:CDS	30.0%
ATCGTAAAACAGTGATCTTT+GGG	+	chr4.2:20942094-20942113	MS.gene28663:intron	30.0%
ATCTTCCATATCCATTTGAT+AGG	-	chr4.2:20941935-20941954	None:intergenic	30.0%
ATGTCAACAAATTGATCTAG+CGG	+	chr4.2:20941708-20941727	MS.gene28663:CDS	30.0%
ATTTGATAGGCATGATAGAA+AGG	-	chr4.2:20941922-20941941	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4.2	gene	20941334	20943253	20941334	ID=MS.gene28663
chr4.2	mRNA	20941334	20943253	20941334	ID=MS.gene28663.t1;Parent=MS.gene28663
chr4.2	exon	20941334	20942020	20941334	ID=MS.gene28663.t1.exon1;Parent=MS.gene28663.t1
chr4.2	CDS	20941334	20942020	20941334	ID=cds.MS.gene28663.t1;Parent=MS.gene28663.t1
chr4.2	exon	20942427	20942641	20942427	ID=MS.gene28663.t1.exon2;Parent=MS.gene28663.t1
chr4.2	CDS	20942427	20942641	20942427	ID=cds.MS.gene28663.t1;Parent=MS.gene28663.t1
chr4.2	exon	20942797	20942833	20942797	ID=MS.gene28663.t1.exon3;Parent=MS.gene28663.t1
chr4.2	CDS	20942797	20942833	20942797	ID=cds.MS.gene28663.t1;Parent=MS.gene28663.t1
chr4.2	exon	20942917	20943048	20942917	ID=MS.gene28663.t1.exon4;Parent=MS.gene28663.t1
chr4.2	CDS	20942917	20943048	20942917	ID=cds.MS.gene28663.t1;Parent=MS.gene28663.t1
chr4.2	exon	20943125	20943253	20943125	ID=MS.gene28663.t1.exon5;Parent=MS.gene28663.t1
chr4.2	CDS	20943125	20943253	20943125	ID=cds.MS.gene28663.t1;Parent=MS.gene28663.t1

Gene Sequence

>MS.gene28663

ATGCAAGAAGATCGGAACCCAAAAAAAGGCATGGTTTTTGATAGCTTTGATGATGCTTGGAAGTTTTGGGTTGATTATGGAGGGAGAGTTGGATTTGGAGTGAGAGTACAATATGTACACAAGAATAAAGACGGCTTAGCCACTTCTTGCAGGTTTGTTTGTTGCAAAGAAGGCCTACGAAAACCAGACAAACGAGATTATAAAACAATCAAGCCAAGAGCAGAGACCAGAACCAACTGCAAAGCAAGGATAGGATTTAAGAATATGGGTGGAGTTTGGACGGTGCATGATTTTGAGCCAGAACATAACCATATTATGCACACTCCAGAAACAACTCATATGTTGTCATCTCAAAGAAAGCTTTCAGAAATTCAATGTCAACAAATTGATCTAGCGGACGATGTCGGTCTCCAACAAAGGAATTCATTTGATTTGATGAGCAAGGAAGTGGGAGGTAGAACCAACTTGGGATTTATACGCCTTGATCAAAAGAATTATCTCCGAAAAAGAAGGGAAAGAAGTTTGGTACTCGGGGAAGCCGACTATCTGTTACAATACTTTCAGAACATGTCAATAGAAAATCACCCTTTCTATCATGCCTATCAAATGGATATGGAAGATCAAATTACCAACGTGTTCTGGGCAGATGCAAGGATGCTCATTGATTATGGGTATTTCGGTGACGTGGTTTCTCTTGATTCAACATATTGTACAAATACTTCACATAGACCATTAGCTGTGTTTTCAGGATTTAACCACCATCGTAAAACAGTGATCTTTGGGGCTGCATTATTGTATGATGAAACAAAAGAGTCATACAAGTGGCTTTTTGAGACATTTTTAGAAGCACACAAGCAGAAAATGCCTCAAACTGTTTTTACTGATCAAGATCAGTCAATGGCAAACGCACTTGTCGAAGTTATGCCTAGAGCATACCATGGCCTATGTACGTGGCATTTGATGCAAAATGGATAAGAATTTGATTAGTTGCTCTTGTCGCAAGTTTGAGAGCTTTGGCATACTATGTTGTCATTGTTTAAAAGTCTTTATTCATATGGATGTGAAAACAGTGCCTGAACATTATATCTTGAAGAGGTGGACAAAGTCAGCAAGAAGCGGGGTCTTACCTATTGTTGATGTTAGCCATGATGTGGAAGATGTTGAATTTTCTCCAAGGAAATGCTACAAGGAGATTTGTCCTCGCTATATCAGGACACCGAAGGATGTAGAAGGCCAGAAACATTCACCTTTCTTACCAAAGTTGCTGATGAGTTGGATAGGCTGGTACTACAGTTTCAAAACTGTCAAGTAAGTAGTGATCAAGAAGATTTGTTTCTTCGCAAAATTAAAGAGATCGGAAGTAGCATTGATGGTTCAACAACAGTCAAGGGTTTTAAAAAGAAAGAAGGAAGAAGAGGTACAAAACGTCTCAAAGGTTTCATAGAATGCAAACTTTCCAACAGAAAGAAAAAAGATGGTCCAAGTGCTTCACAAACAAAGGTATTATTTATTTATGTAATTTTAATTTTACATTTAGTTAAATACAAAAATTCATGTTTTCTGTTATTTATTTTTTTATGCAGAATGATGTTAGAAGGGTAAAAACTACCAAAAAAAGCACAAATGGAGACTTAGGTAATTCACAAATGCAATATGAGGTGCCCAACACAAGTGGAACTATTTGGCTCAATGATGTACACAGTAGATCTTTCATGGTAAGCTAATAGCAATTGGAGTGTGTGAATGAACTAAAAAATGTTCACTGATTAAATATTTTTAATGTTTATGCAGGAAGATAATGCTACTTTATGTTCTTTTGGTGAAGATAATGTCACTTTAAGCTCATTTGGTACCACTAGTTTTACTAGCTTATTGATGGGAAACCTACATGAGGATACCACAAGATTGATGAATCCATAG

Protein sequence

>MS.gene28663.t1

MQEDRNPKKGMVFDSFDDAWKFWVDYGGRVGFGVRVQYVHKNKDGLATSCRFVCCKEGLRKPDKRDYKTIKPRAETRTNCKARIGFKNMGGVWTVHDFEPEHNHIMHTPETTHMLSSQRKLSEIQCQQIDLADDVGLQQRNSFDLMSKEVGGRTNLGFIRLDQKNYLRKRRERSLVLGEADYLLQYFQNMSIENHPFYHAYQMDMEDQITNVFWADARMLIDYGYFGDVRWTKSARSGVLPIVDVSHDVEDVEFSPRKCYKEICPRYIRTPKDVEGQKHSPFLPKLLMSWIGWYYSFKTVKKKKDGPSASQTKNDVRRVKTTKKSTNGDLGNSQMQYEVPNTSGTIWLNDVHSRSFMEDNATLCSFGEDNVTLSSFGTTSFTSLLMGNLHEDTTRLMNP