AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene28663

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28663.t1 MTR_4g102720 54.971 171 44 1 1 171 1 138 4.62e-57 186
MS.gene28663.t1 MTR_5g039760 39.175 194 92 3 10 201 13 182 1.60e-40 142
MS.gene28663.t1 MTR_7g074790 41.618 173 82 2 3 174 6 160 4.44e-38 137
MS.gene28663.t1 MTR_0558s0020 32.231 242 143 4 9 229 38 279 2.54e-37 137
MS.gene28663.t1 MTR_7g061980 50.442 113 55 1 4 115 7 119 7.51e-33 120
MS.gene28663.t1 MTR_0402s0040 41.111 180 102 3 7 183 26 204 9.27e-33 129
MS.gene28663.t1 MTR_1g083640 30.870 230 138 4 9 217 53 282 1.47e-31 121
MS.gene28663.t1 MTR_6g051425 29.961 257 168 6 10 254 20 276 5.25e-30 118
MS.gene28663.t1 MTR_8g446020 34.118 170 110 1 62 229 70 239 1.45e-28 112
MS.gene28663.t1 MTR_6g090505 32.821 195 115 3 51 229 20 214 1.18e-27 112
MS.gene28663.t1 MTR_0337s0040 32.468 154 102 1 62 213 71 224 4.77e-22 94.0
MS.gene28663.t1 MTR_5g015480 41.985 131 72 3 7 134 56 185 1.10e-20 91.3
MS.gene28663.t1 MTR_2g014100 29.000 200 121 4 9 187 46 245 1.55e-20 90.5
MS.gene28663.t1 MTR_1g070355 26.697 221 156 2 13 229 80 298 6.33e-19 89.0
MS.gene28663.t1 MTR_7g113010 38.760 129 64 2 4 130 454 569 2.87e-17 84.0
MS.gene28663.t1 MTR_1g057920 50.633 79 38 1 7 84 34 112 4.21e-17 77.4
MS.gene28663.t1 MTR_6g017200 49.367 79 39 1 7 84 58 136 3.51e-16 75.9
MS.gene28663.t1 MTR_2g070590 43.564 101 54 2 7 104 17 117 5.08e-16 74.3
MS.gene28663.t1 MTR_2g098920 40.000 105 59 4 7 109 80 182 2.62e-15 75.9
MS.gene28663.t1 MTR_4g067290 34.266 143 81 5 7 143 56 191 3.18e-15 75.1
MS.gene28663.t1 MTR_5g076070 23.810 231 170 2 3 229 49 277 3.39e-15 75.9
MS.gene28663.t1 MTR_4g067290 37.903 124 68 4 7 128 56 172 3.80e-15 74.7
MS.gene28663.t1 MTR_4g067290 37.903 124 68 4 7 128 76 192 5.39e-15 74.7
MS.gene28663.t1 MTR_4g067290 34.266 143 81 5 7 143 74 209 5.49e-15 74.7
MS.gene28663.t1 MTR_4g067290 34.266 143 81 5 7 143 76 211 5.49e-15 74.7
MS.gene28663.t1 MTR_4g067290 37.903 124 68 4 7 128 116 232 9.32e-15 74.7
MS.gene28663.t1 MTR_1g055240 33.065 124 81 1 62 183 21 144 1.04e-14 71.6
MS.gene28663.t1 MTR_2g099430 43.678 87 48 1 30 115 26 112 4.12e-14 68.9
MS.gene28663.t1 MTR_0019s0290 31.667 120 80 1 66 183 40 159 4.26e-14 70.1
MS.gene28663.t1 MTR_1g008290 29.496 139 95 2 64 202 9 144 5.36e-13 66.6
MS.gene28663.t1 MTR_4g069880 48.387 62 32 0 4 65 7 68 5.46e-13 63.9
MS.gene28663.t1 MTR_4g040430 28.834 163 95 5 9 152 37 197 2.15e-12 66.2
MS.gene28663.t1 MTR_1g013060 25.943 212 149 4 3 207 20 230 2.84e-12 66.2
MS.gene28663.t1 MTR_7g446520 24.312 218 149 3 13 229 94 296 4.11e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28663.t1 AT4G38180 32.824 262 164 6 4 254 70 330 1.21e-34 136
MS.gene28663.t1 AT4G15090 27.074 229 163 4 4 229 48 275 2.87e-23 102
MS.gene28663.t1 AT4G15090 27.074 229 163 4 4 229 48 275 2.87e-23 102
MS.gene28663.t1 AT4G15090 27.074 229 163 4 4 229 48 275 2.87e-23 102
MS.gene28663.t1 AT4G15090 27.074 229 163 4 4 229 48 275 2.87e-23 102
MS.gene28663.t1 AT4G15090 27.074 229 163 4 4 229 48 275 2.87e-23 102
MS.gene28663.t1 AT1G76320 28.287 251 161 7 11 252 1 241 8.18e-20 92.0
MS.gene28663.t1 AT1G76320 28.287 251 161 7 11 252 1 241 8.19e-20 92.0
MS.gene28663.t1 AT1G76320 28.287 251 161 7 11 252 1 241 8.19e-20 92.0
MS.gene28663.t1 AT1G76320 28.287 251 161 7 11 252 1 241 1.08e-19 91.7
MS.gene28663.t1 AT3G06250 29.114 237 122 8 7 238 190 385 1.05e-16 82.8
MS.gene28663.t1 AT3G06250 29.114 237 122 8 7 238 190 385 1.05e-16 82.8
MS.gene28663.t1 AT3G06250 29.114 237 122 8 7 238 190 385 1.05e-16 82.8
MS.gene28663.t1 AT3G22170 25.417 240 137 6 7 229 70 284 1.18e-16 82.4
MS.gene28663.t1 AT3G22170 25.417 240 137 6 7 229 70 284 1.18e-16 82.4
MS.gene28663.t1 AT5G18960 28.033 239 125 9 7 238 211 409 2.81e-15 78.2
MS.gene28663.t1 AT5G18960 28.033 239 125 9 7 238 211 409 2.81e-15 78.2
MS.gene28663.t1 AT3G59470 43.000 100 54 3 7 104 70 168 1.35e-14 73.6
MS.gene28663.t1 AT3G59470 43.000 100 54 3 7 104 70 168 1.35e-14 73.6
MS.gene28663.t1 AT3G59470 43.000 100 54 3 7 104 82 180 1.88e-14 73.2
MS.gene28663.t1 AT2G27110 25.112 223 140 5 7 228 49 245 1.43e-12 70.1
MS.gene28663.t1 AT2G27110 25.112 223 140 5 7 228 49 245 1.43e-12 70.1
MS.gene28663.t1 AT3G07500 42.000 100 56 2 7 104 33 132 1.93e-12 66.6
MS.gene28663.t1 AT1G10240 22.656 256 176 7 7 253 48 290 9.75e-12 67.0
MS.gene28663.t1 AT1G10240 22.656 256 176 7 7 253 48 290 9.75e-12 67.0

Find 86 sgRNAs with CRISPR-Local

Find 137 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTAAGAAAGGTGAATGTTTC+TGG 0.143239 4.2:-20942567 None:intergenic
ATCAAATTACCAACGTGTTC+TGG 0.222689 4.2:+20941953 MS.gene28663:CDS
TTGATGATGCTTGGAAGTTT+TGG 0.231156 4.2:+20941380 MS.gene28663:CDS
CCTTGCTTTGCAGTTGGTTC+TGG 0.231162 4.2:-20941560 None:intergenic
GACAACATATGAGTTGTTTC+TGG 0.251212 4.2:-20941658 None:intergenic
GCAAGGATGCTCATTGATTA+TGG 0.253058 4.2:+20941982 MS.gene28663:CDS
CTCATTGATTATGGGTATTT+CGG 0.259033 4.2:+20941991 MS.gene28663:CDS
AATGCTACTTTATGTTCTTT+TGG 0.263418 4.2:+20943131 MS.gene28663:CDS
AAGGATAGGATTTAAGAATA+TGG 0.268238 4.2:+20941579 MS.gene28663:CDS
TTAAGAATATGGGTGGAGTT+TGG 0.282368 4.2:+20941590 MS.gene28663:CDS
CCAACACAAGTGGAACTATT+TGG 0.315411 4.2:+20942996 MS.gene28663:CDS
AATGTCACTTTAAGCTCATT+TGG 0.331866 4.2:+20943161 MS.gene28663:CDS
ACGGCTTAGCCACTTCTTGC+AGG 0.335704 4.2:+20941464 MS.gene28663:CDS
TATGGAGGGAGAGTTGGATT+TGG 0.335937 4.2:+20941409 MS.gene28663:CDS
TTGGTTCTGGTCTCTGCTCT+TGG 0.343862 4.2:-20941547 None:intergenic
AATCAAATGAATTCCTTTGT+TGG 0.345771 4.2:-20941744 None:intergenic
TCATGCCTATCAAATGGATA+TGG 0.346715 4.2:+20941927 MS.gene28663:CDS
AGATTTGTCCTCGCTATATC+AGG 0.348152 4.2:+20942524 MS.gene28663:CDS
TTATGCAGAATGATGTTAGA+AGG 0.352354 4.2:+20942909 MS.gene28663:intron
ATCTTCCATATCCATTTGAT+AGG 0.361929 4.2:-20941932 None:intergenic
TTTCTGGAGTGTGCATAATA+TGG 0.369956 4.2:-20941642 None:intergenic
TTCTGGCCTTCTACATCCTT+CGG 0.374919 4.2:-20942550 None:intergenic
ATCTCCTTGTAGCATTTCCT+TGG 0.394339 4.2:-20942505 None:intergenic
TCAAATTACCAACGTGTTCT+GGG 0.414584 4.2:+20941954 MS.gene28663:CDS
AGGTTTGTTTGTTGCAAAGA+AGG 0.419137 4.2:+20941484 MS.gene28663:CDS
TCCTATCCTTGCTTTGCAGT+TGG 0.430816 4.2:-20941566 None:intergenic
TGTACACAGTAGATCTTTCA+TGG 0.432557 4.2:+20943027 MS.gene28663:CDS
ATCCAACTCATCAGCAACTT+TGG 0.438321 4.2:-20942589 None:intergenic
TACCAAAGTTGCTGATGAGT+TGG 0.446669 4.2:+20942587 MS.gene28663:CDS
CAAATAGTTCCACTTGTGTT+GGG 0.450944 4.2:-20942995 None:intergenic
TTTCTATCATGCCTATCAAA+TGG 0.458328 4.2:+20941921 MS.gene28663:CDS
AAAAGCACAAATGGAGACTT+AGG 0.461223 4.2:+20942947 MS.gene28663:CDS
TCATGGCTAACATCAACAAT+AGG 0.466270 4.2:-20942460 None:intergenic
ATCAGCAACTTTGGTAAGAA+AGG 0.468458 4.2:-20942580 None:intergenic
TGATTTGATGAGCAAGGAAG+TGG 0.472654 4.2:+20941762 MS.gene28663:CDS
AGGATAGGATTTAAGAATAT+GGG 0.472781 4.2:+20941580 MS.gene28663:CDS
CCAAATAGTTCCACTTGTGT+TGG 0.476998 4.2:-20942996 None:intergenic
ATTTGATAGGCATGATAGAA+AGG 0.480340 4.2:-20941919 None:intergenic
GTTGATTATGGAGGGAGAGT+TGG 0.484337 4.2:+20941403 MS.gene28663:CDS
CAAGGATGCTCATTGATTAT+GGG 0.485497 4.2:+20941983 MS.gene28663:CDS
GGACAAAGTCAGCAAGAAGC+GGG 0.487089 4.2:+20942431 MS.gene28663:CDS
TGCATAATATGGTTATGTTC+TGG 0.490959 4.2:-20941631 None:intergenic
TGCTGATGAGTTGGATAGGC+TGG 0.501292 4.2:+20942596 MS.gene28663:CDS
ACCTTTGTTTGTGAAGCACT+TGG 0.506363 4.2:-20942813 None:intergenic
TTGATAGCTTTGATGATGCT+TGG 0.507235 4.2:+20941371 MS.gene28663:CDS
GGGAAAGAAGTTTGGTACTC+GGG 0.507423 4.2:+20941845 MS.gene28663:CDS
AGGACACCGAAGGATGTAGA+AGG 0.508413 4.2:+20942544 MS.gene28663:CDS
TTCTCCAAGGAAATGCTACA+AGG 0.515443 4.2:+20942501 MS.gene28663:CDS
TATGCAGAATGATGTTAGAA+GGG 0.516550 4.2:+20942910 MS.gene28663:intron
TTCATTTGATTTGATGAGCA+AGG 0.524241 4.2:+20941756 MS.gene28663:CDS
ACGATGTCGGTCTCCAACAA+AGG 0.528410 4.2:+20941731 MS.gene28663:CDS
TAATTCACAAATGCAATATG+AGG 0.531476 4.2:+20942970 MS.gene28663:CDS
AGTGGGAGGTAGAACCAACT+TGG 0.541339 4.2:+20941780 MS.gene28663:CDS
ATTCAACATCTTCCACATCA+TGG 0.547232 4.2:-20942477 None:intergenic
AAAGTATTGTAACAGATAGT+CGG 0.556314 4.2:-20941872 None:intergenic
GATACAAAATGCAAGAAGAT+CGG 0.560009 4.2:+20941326 None:intergenic
ATCTTGTGGTATCCTCATGT+AGG 0.561243 4.2:-20943217 None:intergenic
CTATGGATTCATCAATCTTG+TGG 0.565004 4.2:-20943231 None:intergenic
TTTGATAGGCATGATAGAAA+GGG 0.565210 4.2:-20941918 None:intergenic
AGGGAAAGAAGTTTGGTACT+CGG 0.565522 4.2:+20941844 MS.gene28663:CDS
ACGTGTTCTGGGCAGATGCA+AGG 0.571262 4.2:+20941965 MS.gene28663:CDS
GAATATGGGTGGAGTTTGGA+CGG 0.573195 4.2:+20941594 MS.gene28663:CDS
ACAAACAAACCTGCAAGAAG+TGG 0.580136 4.2:-20941473 None:intergenic
TATGAGGTGCCCAACACAAG+TGG 0.584247 4.2:+20942986 MS.gene28663:CDS
ATTGATCTAGCGGACGATGT+CGG 0.586585 4.2:+20941718 MS.gene28663:CDS
GATTTGATGAGCAAGGAAGT+GGG 0.590244 4.2:+20941763 MS.gene28663:CDS
AAGGCGTATAAATCCCAAGT+TGG 0.596512 4.2:-20941794 None:intergenic
TATGTACACAAGAATAAAGA+CGG 0.602767 4.2:+20941445 MS.gene28663:CDS
TCCAAGTGCTTCACAAACAA+AGG 0.608130 4.2:+20942812 MS.gene28663:CDS
TCGCTATATCAGGACACCGA+AGG 0.609157 4.2:+20942534 MS.gene28663:CDS
CCAGAACCAACTGCAAAGCA+AGG 0.609490 4.2:+20941560 MS.gene28663:CDS
ATAGGATTTAAGAATATGGG+TGG 0.612678 4.2:+20941583 MS.gene28663:CDS
TTATGGGTATTTCGGTGACG+TGG 0.614423 4.2:+20941999 MS.gene28663:CDS
TGTTGATGTTAGCCATGATG+TGG 0.621134 4.2:+20942465 MS.gene28663:CDS
ACCAACTGCAAAGCAAGGAT+AGG 0.623297 4.2:+20941565 MS.gene28663:CDS
ATGTCAACAAATTGATCTAG+CGG 0.626650 4.2:+20941708 MS.gene28663:CDS
TGGACAAAGTCAGCAAGAAG+CGG 0.632418 4.2:+20942430 MS.gene28663:CDS
GGAAAGAAGTTTGGTACTCG+GGG 0.643149 4.2:+20941846 MS.gene28663:CDS
AAGTTGCTGATGAGTTGGAT+AGG 0.645499 4.2:+20942592 MS.gene28663:CDS
CAATAAGCTAGTAAAACTAG+TGG 0.648351 4.2:-20943186 None:intergenic
TTCGGTGTCCTGATATAGCG+AGG 0.651118 4.2:-20942532 None:intergenic
GTGGGAGGTAGAACCAACTT+GGG 0.659665 4.2:+20941781 MS.gene28663:CDS
GACAAAGTCAGCAAGAAGCG+GGG 0.678202 4.2:+20942432 MS.gene28663:CDS
TTGATGAGCAAGGAAGTGGG+AGG 0.684139 4.2:+20941766 MS.gene28663:CDS
ATTGATGGGAAACCTACATG+AGG 0.691112 4.2:+20943205 MS.gene28663:CDS
TGCATCTGCCCAGAACACGT+TGG 0.710148 4.2:-20941962 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTAAAAGTCTTTATTCATA+TGG + chr4.2:20942369-20942388 MS.gene28663:intron 15.0%
!!! AATATTTTTAATGTTTATGC+AGG + chr4.2:20943103-20943122 MS.gene28663:intron 15.0%
! AAGGATAGGATTTAAGAATA+TGG + chr4.2:20941579-20941598 MS.gene28663:CDS 25.0%
! AATCAAATGAATTCCTTTGT+TGG - chr4.2:20941747-20941766 None:intergenic 25.0%
! AGGATAGGATTTAAGAATAT+GGG + chr4.2:20941580-20941599 MS.gene28663:CDS 25.0%
! TAATTCACAAATGCAATATG+AGG + chr4.2:20942970-20942989 MS.gene28663:CDS 25.0%
! TATGTACACAAGAATAAAGA+CGG + chr4.2:20941445-20941464 MS.gene28663:CDS 25.0%
!!! AAAGTATTGTAACAGATAGT+CGG - chr4.2:20941875-20941894 None:intergenic 25.0%
!!! AAGGGTTTTAAAAAGAAAGA+AGG + chr4.2:20942720-20942739 MS.gene28663:intron 25.0%
!!! AATGCTACTTTATGTTCTTT+TGG + chr4.2:20943131-20943150 MS.gene28663:CDS 25.0%
!!! AGTTTTACTAGCTTATTGAT+GGG + chr4.2:20943191-20943210 MS.gene28663:CDS 25.0%
!!! TAGTTTTACTAGCTTATTGA+TGG + chr4.2:20943190-20943209 MS.gene28663:CDS 25.0%
AAAAAGAAAGAAGGAAGAAG+AGG + chr4.2:20942729-20942748 MS.gene28663:intron 30.0%
AAAAAGAAGGGAAAGAAGTT+TGG + chr4.2:20941837-20941856 MS.gene28663:CDS 30.0%
AAACAAAAGAGTCATACAAG+TGG + chr4.2:20942136-20942155 MS.gene28663:intron 30.0%
AACATTATATCTTGAAGAGG+TGG + chr4.2:20942410-20942429 MS.gene28663:intron 30.0%
AGAATTATCTCCGAAAAAGA+AGG + chr4.2:20941824-20941843 MS.gene28663:CDS 30.0%
ATAGGATTTAAGAATATGGG+TGG + chr4.2:20941583-20941602 MS.gene28663:CDS 30.0%
ATCGTAAAACAGTGATCTTT+GGG + chr4.2:20942094-20942113 MS.gene28663:intron 30.0%
ATCTTCCATATCCATTTGAT+AGG - chr4.2:20941935-20941954 None:intergenic 30.0%
ATGTCAACAAATTGATCTAG+CGG + chr4.2:20941708-20941727 MS.gene28663:CDS 30.0%
ATTTGATAGGCATGATAGAA+AGG - chr4.2:20941922-20941941 None:intergenic 30.0%
CAATAAGCTAGTAAAACTAG+TGG - chr4.2:20943189-20943208 None:intergenic 30.0%
CTCTTCAAGATATAATGTTC+AGG - chr4.2:20942409-20942428 None:intergenic 30.0%
CTGAACATTATATCTTGAAG+AGG + chr4.2:20942407-20942426 MS.gene28663:intron 30.0%
CTTCGCAAAATTAAAGAGAT+CGG + chr4.2:20942669-20942688 MS.gene28663:intron 30.0%
GAATTATCTCCGAAAAAGAA+GGG + chr4.2:20941825-20941844 MS.gene28663:CDS 30.0%
TATGCAGAATGATGTTAGAA+GGG + chr4.2:20942910-20942929 MS.gene28663:intron 30.0%
TCCAACAGAAAGAAAAAAGA+TGG + chr4.2:20942789-20942808 MS.gene28663:intron 30.0%
TGATCAGTAAAAACAGTTTG+AGG - chr4.2:20942201-20942220 None:intergenic 30.0%
TGCATAATATGGTTATGTTC+TGG - chr4.2:20941634-20941653 None:intergenic 30.0%
TTATGCAGAATGATGTTAGA+AGG + chr4.2:20942909-20942928 MS.gene28663:intron 30.0%
TTTCTATCATGCCTATCAAA+TGG + chr4.2:20941921-20941940 MS.gene28663:CDS 30.0%
TTTGATAGGCATGATAGAAA+GGG - chr4.2:20941921-20941940 None:intergenic 30.0%
! AACTTCTTTCCCTTCTTTTT+CGG - chr4.2:20941837-20941856 None:intergenic 30.0%
! AAGATGTTGAATTTTCTCCA+AGG + chr4.2:20942488-20942507 MS.gene28663:CDS 30.0%
! ACTACCAAAAAAAGCACAAA+TGG + chr4.2:20942938-20942957 MS.gene28663:CDS 30.0%
! CTCATTGATTATGGGTATTT+CGG + chr4.2:20941991-20942010 MS.gene28663:CDS 30.0%
! GGAGATAATTCTTTTGATCA+AGG - chr4.2:20941816-20941835 None:intergenic 30.0%
! GTTTTATAATCTCGTTTGTC+TGG - chr4.2:20941520-20941539 None:intergenic 30.0%
!! AATGTCACTTTAAGCTCATT+TGG + chr4.2:20943161-20943180 MS.gene28663:CDS 30.0%
!! TTCATTTGATTTGATGAGCA+AGG + chr4.2:20941756-20941775 MS.gene28663:CDS 30.0%
!!! ACCATCTTTTTTCTTTCTGT+TGG - chr4.2:20942793-20942812 None:intergenic 30.0%
!!! CAAAAACCATGCCTTTTTTT+GGG - chr4.2:20941354-20941373 None:intergenic 30.0%
!!! TCAAAAACCATGCCTTTTTT+TGG - chr4.2:20941355-20941374 None:intergenic 30.0%
AATCCTGAAAACACAGCTAA+TGG - chr4.2:20942066-20942085 None:intergenic 35.0%
ATCAAATTACCAACGTGTTC+TGG + chr4.2:20941953-20941972 MS.gene28663:CDS 35.0%
ATCAGCAACTTTGGTAAGAA+AGG - chr4.2:20942583-20942602 None:intergenic 35.0%
ATTCAACATCTTCCACATCA+TGG - chr4.2:20942480-20942499 None:intergenic 35.0%
CAAATAGTTCCACTTGTGTT+GGG - chr4.2:20942998-20943017 None:intergenic 35.0%
CATCGTAAAACAGTGATCTT+TGG + chr4.2:20942093-20942112 MS.gene28663:intron 35.0%
CATGGTAAGCTAATAGCAAT+TGG + chr4.2:20943045-20943064 MS.gene28663:intron 35.0%
CTATGGATTCATCAATCTTG+TGG - chr4.2:20943234-20943253 None:intergenic 35.0%
GTAAGAAAGGTGAATGTTTC+TGG - chr4.2:20942570-20942589 None:intergenic 35.0%
TACTGATCAAGATCAGTCAA+TGG + chr4.2:20942212-20942231 MS.gene28663:intron 35.0%
TCAAATTACCAACGTGTTCT+GGG + chr4.2:20941954-20941973 MS.gene28663:CDS 35.0%
TCATGCCTATCAAATGGATA+TGG + chr4.2:20941927-20941946 MS.gene28663:CDS 35.0%
TCATGGCTAACATCAACAAT+AGG - chr4.2:20942463-20942482 None:intergenic 35.0%
TCGTAAAACAGTGATCTTTG+GGG + chr4.2:20942095-20942114 MS.gene28663:intron 35.0%
TGTACACAGTAGATCTTTCA+TGG + chr4.2:20943027-20943046 MS.gene28663:CDS 35.0%
TTAAGAATATGGGTGGAGTT+TGG + chr4.2:20941590-20941609 MS.gene28663:CDS 35.0%
! AAAAGCACAAATGGAGACTT+AGG + chr4.2:20942947-20942966 MS.gene28663:CDS 35.0%
! AGGTTTGTTTGTTGCAAAGA+AGG + chr4.2:20941484-20941503 MS.gene28663:CDS 35.0%
! CAAAGATCACTGTTTTACGA+TGG - chr4.2:20942095-20942114 None:intergenic 35.0%
! CAAGGATGCTCATTGATTAT+GGG + chr4.2:20941983-20942002 MS.gene28663:CDS 35.0%
! GACAACATATGAGTTGTTTC+TGG - chr4.2:20941661-20941680 None:intergenic 35.0%
!! TGATGATGCTTGGAAGTTTT+GGG + chr4.2:20941381-20941400 MS.gene28663:CDS 35.0%
!! TTGATAGCTTTGATGATGCT+TGG + chr4.2:20941371-20941390 MS.gene28663:CDS 35.0%
!! TTTCTGGAGTGTGCATAATA+TGG - chr4.2:20941645-20941664 None:intergenic 35.0%
!!! AAGTTTTGGGTTGATTATGG+AGG + chr4.2:20941394-20941413 MS.gene28663:CDS 35.0%
!!! AGTTTTGGGTTGATTATGGA+GGG + chr4.2:20941395-20941414 MS.gene28663:CDS 35.0%
!!! GTCTCCATTTGTGCTTTTTT+TGG - chr4.2:20942945-20942964 None:intergenic 35.0%
!!! TGGAAGTTTTGGGTTGATTA+TGG + chr4.2:20941391-20941410 MS.gene28663:CDS 35.0%
!!! TTGATGATGCTTGGAAGTTT+TGG + chr4.2:20941380-20941399 MS.gene28663:CDS 35.0%
AAGGCGTATAAATCCCAAGT+TGG - chr4.2:20941797-20941816 None:intergenic 40.0%
ACAAACAAACCTGCAAGAAG+TGG - chr4.2:20941476-20941495 None:intergenic 40.0%
ACGTGGCATTTGATGCAAAA+TGG + chr4.2:20942282-20942301 MS.gene28663:intron 40.0%
AGATTTGTCCTCGCTATATC+AGG + chr4.2:20942524-20942543 MS.gene28663:CDS 40.0%
AGGGAAAGAAGTTTGGTACT+CGG + chr4.2:20941844-20941863 MS.gene28663:CDS 40.0%
ATCCAACTCATCAGCAACTT+TGG - chr4.2:20942592-20942611 None:intergenic 40.0%
ATCTCCTTGTAGCATTTCCT+TGG - chr4.2:20942508-20942527 None:intergenic 40.0%
ATCTTGTGGTATCCTCATGT+AGG - chr4.2:20943220-20943239 None:intergenic 40.0%
ATTGATGGGAAACCTACATG+AGG + chr4.2:20943205-20943224 MS.gene28663:CDS 40.0%
CCAAATAGTTCCACTTGTGT+TGG - chr4.2:20942999-20943018 None:intergenic 40.0%
CCAACACAAGTGGAACTATT+TGG + chr4.2:20942996-20943015 MS.gene28663:CDS 40.0%
GAAGATCGGAACCCAAAAAA+AGG + chr4.2:20941340-20941359 MS.gene28663:CDS 40.0%
GATGGTTCAACAACAGTCAA+GGG + chr4.2:20942702-20942721 MS.gene28663:intron 40.0%
GATTTGATGAGCAAGGAAGT+GGG + chr4.2:20941763-20941782 MS.gene28663:CDS 40.0%
TACCAAAGTTGCTGATGAGT+TGG + chr4.2:20942587-20942606 MS.gene28663:CDS 40.0%
TCCAAGTGCTTCACAAACAA+AGG + chr4.2:20942812-20942831 MS.gene28663:CDS 40.0%
TGATGGTTCAACAACAGTCA+AGG + chr4.2:20942701-20942720 MS.gene28663:intron 40.0%
TGATTTGATGAGCAAGGAAG+TGG + chr4.2:20941762-20941781 MS.gene28663:CDS 40.0%
TGTTGATGTTAGCCATGATG+TGG + chr4.2:20942465-20942484 MS.gene28663:CDS 40.0%
TTCTCCAAGGAAATGCTACA+AGG + chr4.2:20942501-20942520 MS.gene28663:CDS 40.0%
! AAGTTGCTGATGAGTTGGAT+AGG + chr4.2:20942592-20942611 MS.gene28663:CDS 40.0%
! ACCTTTGTTTGTGAAGCACT+TGG - chr4.2:20942816-20942835 None:intergenic 40.0%
! AGACCATTAGCTGTGTTTTC+AGG + chr4.2:20942060-20942079 MS.gene28663:intron 40.0%
! AGAGGTACAAAACGTCTCAA+AGG + chr4.2:20942747-20942766 MS.gene28663:intron 40.0%
! AGATCACTGTTTTACGATGG+TGG - chr4.2:20942092-20942111 None:intergenic 40.0%
! GCAAGGATGCTCATTGATTA+TGG + chr4.2:20941982-20942001 MS.gene28663:CDS 40.0%
ACCAACTGCAAAGCAAGGAT+AGG + chr4.2:20941565-20941584 MS.gene28663:CDS 45.0%
GAATATGGGTGGAGTTTGGA+CGG + chr4.2:20941594-20941613 MS.gene28663:CDS 45.0%
GCATCAAATGCCACGTACAT+AGG - chr4.2:20942278-20942297 None:intergenic 45.0%
GGAAAGAAGTTTGGTACTCG+GGG + chr4.2:20941846-20941865 MS.gene28663:CDS 45.0%
GGGAAAGAAGTTTGGTACTC+GGG + chr4.2:20941845-20941864 MS.gene28663:CDS 45.0%
GTTATGCCTAGAGCATACCA+TGG + chr4.2:20942252-20942271 MS.gene28663:intron 45.0%
GTTGATTATGGAGGGAGAGT+TGG + chr4.2:20941403-20941422 MS.gene28663:CDS 45.0%
TATGGAGGGAGAGTTGGATT+TGG + chr4.2:20941409-20941428 MS.gene28663:CDS 45.0%
TCCTATCCTTGCTTTGCAGT+TGG - chr4.2:20941569-20941588 None:intergenic 45.0%
TCGGAACCCAAAAAAAGGCA+TGG + chr4.2:20941345-20941364 MS.gene28663:CDS 45.0%
TGGACAAAGTCAGCAAGAAG+CGG + chr4.2:20942430-20942449 MS.gene28663:CDS 45.0%
TGTCGCAAGTTTGAGAGCTT+TGG + chr4.2:20942328-20942347 MS.gene28663:intron 45.0%
TTATGGGTATTTCGGTGACG+TGG + chr4.2:20941999-20942018 MS.gene28663:CDS 45.0%
! ATTGATCTAGCGGACGATGT+CGG + chr4.2:20941718-20941737 MS.gene28663:CDS 45.0%
! GAGATCGGAAGTAGCATTGA+TGG + chr4.2:20942684-20942703 MS.gene28663:intron 45.0%
! TTCTGGCCTTCTACATCCTT+CGG - chr4.2:20942553-20942572 None:intergenic 45.0%
!!! CTCGTTTGTCTGGTTTTCGT+AGG - chr4.2:20941510-20941529 None:intergenic 45.0%
AATGCCACGTACATAGGCCA+TGG - chr4.2:20942272-20942291 None:intergenic 50.0%
ACGATGTCGGTCTCCAACAA+AGG + chr4.2:20941731-20941750 MS.gene28663:CDS 50.0%
AGGACACCGAAGGATGTAGA+AGG + chr4.2:20942544-20942563 MS.gene28663:CDS 50.0%
AGTGGGAGGTAGAACCAACT+TGG + chr4.2:20941780-20941799 MS.gene28663:CDS 50.0%
CATACCATGGCCTATGTACG+TGG + chr4.2:20942265-20942284 MS.gene28663:intron 50.0%
CATAGGCCATGGTATGCTCT+AGG - chr4.2:20942261-20942280 None:intergenic 50.0%
CCAGAACCAACTGCAAAGCA+AGG + chr4.2:20941560-20941579 MS.gene28663:CDS 50.0%
GACAAAGTCAGCAAGAAGCG+GGG + chr4.2:20942432-20942451 MS.gene28663:CDS 50.0%
GGACAAAGTCAGCAAGAAGC+GGG + chr4.2:20942431-20942450 MS.gene28663:CDS 50.0%
GTGGGAGGTAGAACCAACTT+GGG + chr4.2:20941781-20941800 MS.gene28663:CDS 50.0%
TCGCTATATCAGGACACCGA+AGG + chr4.2:20942534-20942553 MS.gene28663:CDS 50.0%
TTGATGAGCAAGGAAGTGGG+AGG + chr4.2:20941766-20941785 MS.gene28663:CDS 50.0%
! CCTTGCTTTGCAGTTGGTTC+TGG - chr4.2:20941563-20941582 None:intergenic 50.0%
! TATGAGGTGCCCAACACAAG+TGG + chr4.2:20942986-20943005 MS.gene28663:CDS 50.0%
! TGCTGATGAGTTGGATAGGC+TGG + chr4.2:20942596-20942615 MS.gene28663:CDS 50.0%
!! TTCGGTGTCCTGATATAGCG+AGG - chr4.2:20942535-20942554 None:intergenic 50.0%
!! TTGGTTCTGGTCTCTGCTCT+TGG - chr4.2:20941550-20941569 None:intergenic 50.0%
ACGGCTTAGCCACTTCTTGC+AGG + chr4.2:20941464-20941483 MS.gene28663:CDS 55.0%
TGCATCTGCCCAGAACACGT+TGG - chr4.2:20941965-20941984 None:intergenic 55.0%
! ACGTGTTCTGGGCAGATGCA+AGG + chr4.2:20941965-20941984 MS.gene28663:CDS 55.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 20941334 20943253 20941334 ID=MS.gene28663
chr4.2 mRNA 20941334 20943253 20941334 ID=MS.gene28663.t1;Parent=MS.gene28663
chr4.2 exon 20941334 20942020 20941334 ID=MS.gene28663.t1.exon1;Parent=MS.gene28663.t1
chr4.2 CDS 20941334 20942020 20941334 ID=cds.MS.gene28663.t1;Parent=MS.gene28663.t1
chr4.2 exon 20942427 20942641 20942427 ID=MS.gene28663.t1.exon2;Parent=MS.gene28663.t1
chr4.2 CDS 20942427 20942641 20942427 ID=cds.MS.gene28663.t1;Parent=MS.gene28663.t1
chr4.2 exon 20942797 20942833 20942797 ID=MS.gene28663.t1.exon3;Parent=MS.gene28663.t1
chr4.2 CDS 20942797 20942833 20942797 ID=cds.MS.gene28663.t1;Parent=MS.gene28663.t1
chr4.2 exon 20942917 20943048 20942917 ID=MS.gene28663.t1.exon4;Parent=MS.gene28663.t1
chr4.2 CDS 20942917 20943048 20942917 ID=cds.MS.gene28663.t1;Parent=MS.gene28663.t1
chr4.2 exon 20943125 20943253 20943125 ID=MS.gene28663.t1.exon5;Parent=MS.gene28663.t1
chr4.2 CDS 20943125 20943253 20943125 ID=cds.MS.gene28663.t1;Parent=MS.gene28663.t1
Gene Sequence

>MS.gene28663

ATGCAAGAAGATCGGAACCCAAAAAAAGGCATGGTTTTTGATAGCTTTGATGATGCTTGGAAGTTTTGGGTTGATTATGGAGGGAGAGTTGGATTTGGAGTGAGAGTACAATATGTACACAAGAATAAAGACGGCTTAGCCACTTCTTGCAGGTTTGTTTGTTGCAAAGAAGGCCTACGAAAACCAGACAAACGAGATTATAAAACAATCAAGCCAAGAGCAGAGACCAGAACCAACTGCAAAGCAAGGATAGGATTTAAGAATATGGGTGGAGTTTGGACGGTGCATGATTTTGAGCCAGAACATAACCATATTATGCACACTCCAGAAACAACTCATATGTTGTCATCTCAAAGAAAGCTTTCAGAAATTCAATGTCAACAAATTGATCTAGCGGACGATGTCGGTCTCCAACAAAGGAATTCATTTGATTTGATGAGCAAGGAAGTGGGAGGTAGAACCAACTTGGGATTTATACGCCTTGATCAAAAGAATTATCTCCGAAAAAGAAGGGAAAGAAGTTTGGTACTCGGGGAAGCCGACTATCTGTTACAATACTTTCAGAACATGTCAATAGAAAATCACCCTTTCTATCATGCCTATCAAATGGATATGGAAGATCAAATTACCAACGTGTTCTGGGCAGATGCAAGGATGCTCATTGATTATGGGTATTTCGGTGACGTGGTTTCTCTTGATTCAACATATTGTACAAATACTTCACATAGACCATTAGCTGTGTTTTCAGGATTTAACCACCATCGTAAAACAGTGATCTTTGGGGCTGCATTATTGTATGATGAAACAAAAGAGTCATACAAGTGGCTTTTTGAGACATTTTTAGAAGCACACAAGCAGAAAATGCCTCAAACTGTTTTTACTGATCAAGATCAGTCAATGGCAAACGCACTTGTCGAAGTTATGCCTAGAGCATACCATGGCCTATGTACGTGGCATTTGATGCAAAATGGATAAGAATTTGATTAGTTGCTCTTGTCGCAAGTTTGAGAGCTTTGGCATACTATGTTGTCATTGTTTAAAAGTCTTTATTCATATGGATGTGAAAACAGTGCCTGAACATTATATCTTGAAGAGGTGGACAAAGTCAGCAAGAAGCGGGGTCTTACCTATTGTTGATGTTAGCCATGATGTGGAAGATGTTGAATTTTCTCCAAGGAAATGCTACAAGGAGATTTGTCCTCGCTATATCAGGACACCGAAGGATGTAGAAGGCCAGAAACATTCACCTTTCTTACCAAAGTTGCTGATGAGTTGGATAGGCTGGTACTACAGTTTCAAAACTGTCAAGTAAGTAGTGATCAAGAAGATTTGTTTCTTCGCAAAATTAAAGAGATCGGAAGTAGCATTGATGGTTCAACAACAGTCAAGGGTTTTAAAAAGAAAGAAGGAAGAAGAGGTACAAAACGTCTCAAAGGTTTCATAGAATGCAAACTTTCCAACAGAAAGAAAAAAGATGGTCCAAGTGCTTCACAAACAAAGGTATTATTTATTTATGTAATTTTAATTTTACATTTAGTTAAATACAAAAATTCATGTTTTCTGTTATTTATTTTTTTATGCAGAATGATGTTAGAAGGGTAAAAACTACCAAAAAAAGCACAAATGGAGACTTAGGTAATTCACAAATGCAATATGAGGTGCCCAACACAAGTGGAACTATTTGGCTCAATGATGTACACAGTAGATCTTTCATGGTAAGCTAATAGCAATTGGAGTGTGTGAATGAACTAAAAAATGTTCACTGATTAAATATTTTTAATGTTTATGCAGGAAGATAATGCTACTTTATGTTCTTTTGGTGAAGATAATGTCACTTTAAGCTCATTTGGTACCACTAGTTTTACTAGCTTATTGATGGGAAACCTACATGAGGATACCACAAGATTGATGAATCCATAG
Protein sequence

>MS.gene28663.t1

MQEDRNPKKGMVFDSFDDAWKFWVDYGGRVGFGVRVQYVHKNKDGLATSCRFVCCKEGLRKPDKRDYKTIKPRAETRTNCKARIGFKNMGGVWTVHDFEPEHNHIMHTPETTHMLSSQRKLSEIQCQQIDLADDVGLQQRNSFDLMSKEVGGRTNLGFIRLDQKNYLRKRRERSLVLGEADYLLQYFQNMSIENHPFYHAYQMDMEDQITNVFWADARMLIDYGYFGDVRWTKSARSGVLPIVDVSHDVEDVEFSPRKCYKEICPRYIRTPKDVEGQKHSPFLPKLLMSWIGWYYSFKTVKKKKDGPSASQTKNDVRRVKTTKKSTNGDLGNSQMQYEVPNTSGTIWLNDVHSRSFMEDNATLCSFGEDNVTLSSFGTTSFTSLLMGNLHEDTTRLMNP