Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28698.t1 | XP_013457137.1 | 96.7 | 210 | 7 | 0 | 1 | 210 | 1 | 210 | 2.70E-105 | 391.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28698.t1 | O81900 | 42.5 | 179 | 86 | 3 | 24 | 192 | 14 | 185 | 1.3e-26 | 121.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28698.t1 | A0A072UQ23 | 96.7 | 210 | 7 | 0 | 1 | 210 | 1 | 210 | 2.0e-105 | 391.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene28698.t1 | TF | bHLH |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28698.t1 | MTR_4g091910 | 96.667 | 210 | 7 | 0 | 1 | 210 | 1 | 210 | 7.10e-151 | 417 |
| MS.gene28698.t1 | MTR_4g091990 | 47.867 | 211 | 90 | 4 | 1 | 209 | 1 | 193 | 2.77e-60 | 187 |
| MS.gene28698.t1 | MTR_8g064700 | 31.429 | 175 | 106 | 3 | 42 | 202 | 239 | 413 | 1.15e-19 | 86.3 |
| MS.gene28698.t1 | MTR_1g112680 | 30.526 | 190 | 92 | 4 | 27 | 183 | 304 | 486 | 8.73e-18 | 81.3 |
| MS.gene28698.t1 | MTR_2g084280 | 31.737 | 167 | 109 | 3 | 37 | 198 | 121 | 287 | 1.07e-15 | 74.3 |
| MS.gene28698.t1 | MTR_4g057270 | 29.940 | 167 | 96 | 5 | 46 | 197 | 148 | 308 | 2.22e-15 | 73.9 |
| MS.gene28698.t1 | MTR_2g038040 | 27.108 | 166 | 103 | 2 | 45 | 192 | 50 | 215 | 1.31e-11 | 62.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28698.t1 | AT4G21330 | 45.283 | 159 | 75 | 2 | 44 | 192 | 29 | 185 | 8.18e-39 | 133 |
| MS.gene28698.t1 | AT2G16910 | 32.298 | 161 | 82 | 3 | 46 | 179 | 313 | 473 | 9.62e-18 | 81.3 |
| MS.gene28698.t1 | AT5G57150 | 30.769 | 169 | 103 | 3 | 38 | 192 | 46 | 214 | 1.71e-14 | 69.7 |
| MS.gene28698.t1 | AT5G57150 | 30.769 | 169 | 103 | 3 | 38 | 192 | 45 | 213 | 1.92e-14 | 69.7 |
| MS.gene28698.t1 | AT5G57150 | 30.769 | 169 | 103 | 3 | 38 | 192 | 46 | 214 | 2.10e-14 | 69.7 |
| MS.gene28698.t1 | AT5G57150 | 30.769 | 169 | 103 | 3 | 38 | 192 | 45 | 213 | 3.38e-14 | 69.7 |
| MS.gene28698.t1 | AT5G57150 | 30.769 | 169 | 103 | 3 | 38 | 192 | 46 | 214 | 4.37e-14 | 69.3 |
| MS.gene28698.t1 | AT2G28160 | 27.869 | 183 | 119 | 5 | 21 | 191 | 103 | 284 | 6.81e-13 | 66.6 |
| MS.gene28698.t1 | AT2G28160 | 27.869 | 183 | 119 | 5 | 21 | 191 | 103 | 284 | 7.90e-13 | 66.6 |
| MS.gene28698.t1 | AT1G10610 | 28.571 | 147 | 96 | 1 | 44 | 181 | 231 | 377 | 7.99e-13 | 67.0 |
| MS.gene28698.t1 | AT1G10610 | 28.571 | 147 | 96 | 1 | 44 | 181 | 261 | 407 | 1.00e-12 | 66.6 |
| MS.gene28698.t1 | AT1G10610 | 28.571 | 147 | 96 | 1 | 44 | 181 | 164 | 310 | 1.39e-12 | 65.9 |
| MS.gene28698.t1 | AT1G12860 | 29.870 | 154 | 93 | 2 | 46 | 186 | 266 | 417 | 2.82e-12 | 65.5 |
| MS.gene28698.t1 | AT1G12860 | 29.870 | 154 | 93 | 2 | 46 | 186 | 266 | 417 | 2.92e-12 | 65.1 |
| MS.gene28698.t1 | AT3G26744 | 30.769 | 156 | 93 | 3 | 46 | 186 | 306 | 461 | 2.50e-11 | 62.4 |
| MS.gene28698.t1 | AT3G26744 | 30.769 | 156 | 93 | 3 | 46 | 186 | 306 | 461 | 2.58e-11 | 62.4 |
| MS.gene28698.t1 | AT3G26744 | 30.769 | 156 | 93 | 3 | 46 | 186 | 306 | 461 | 2.58e-11 | 62.4 |
| MS.gene28698.t1 | AT3G26744 | 30.769 | 156 | 93 | 3 | 46 | 186 | 306 | 461 | 2.58e-11 | 62.4 |
| MS.gene28698.t1 | AT4G37850 | 31.818 | 154 | 80 | 4 | 51 | 188 | 156 | 300 | 2.82e-11 | 62.0 |
| MS.gene28698.t1 | AT4G37850 | 31.818 | 154 | 80 | 4 | 51 | 188 | 156 | 300 | 2.82e-11 | 62.0 |
Find 43 sgRNAs with CRISPR-Local
Find 64 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCAACTATTATCAAGTATT+AGG | 0.172705 | 4.3:+22338959 | MS.gene28698:CDS |
| TCTTTCTGTCTCAAGGTTCT+TGG | 0.203142 | 4.3:-22337795 | None:intergenic |
| TTCTTGGATGTGAACACTTT+TGG | 0.236396 | 4.3:-22337779 | None:intergenic |
| GATGAATAAAATGGGGTTAC+AGG | 0.250695 | 4.3:+22338450 | MS.gene28698:CDS |
| AACTTCATGTTGCAACTTCA+TGG | 0.287708 | 4.3:-22338338 | None:intergenic |
| TGTTGATGAGATGAATAAAA+TGG | 0.288149 | 4.3:+22338441 | MS.gene28698:CDS |
| TTGGGTTTCAATGCTGAAAA+TGG | 0.303250 | 4.3:+22337704 | MS.gene28698:CDS |
| GCAGAAGACTCTCTTTGTTT+TGG | 0.306313 | 4.3:-22338418 | None:intergenic |
| CCAACTATTATCAAGTATTA+GGG | 0.313449 | 4.3:+22338960 | MS.gene28698:CDS |
| CCCTAATACTTGATAATAGT+TGG | 0.313701 | 4.3:-22338960 | None:intergenic |
| CAATAGACTATGTGTGTCTA+TGG | 0.334837 | 4.3:+22337676 | MS.gene28698:CDS |
| TTCTCTTTCTTTCTGTCTCA+AGG | 0.354427 | 4.3:-22337802 | None:intergenic |
| ATGCTGAAAATGGTAGCAAT+AGG | 0.358285 | 4.3:+22337714 | MS.gene28698:CDS |
| TCTCATTACAGCCTCCTTGC+AGG | 0.362149 | 4.3:+22338721 | MS.gene28698:CDS |
| ATGGTAGCAATAGGGGAAAC+TGG | 0.388089 | 4.3:+22337723 | MS.gene28698:CDS |
| GAAACTCACTAGCAGACTTT+TGG | 0.388264 | 4.3:+22337829 | MS.gene28698:CDS |
| GTGCTTACATTTGTGATGAT+TGG | 0.399278 | 4.3:-22337869 | None:intergenic |
| TGCTGAAAATGGTAGCAATA+GGG | 0.418097 | 4.3:+22337715 | MS.gene28698:CDS |
| GTTGATGAGATGAATAAAAT+GGG | 0.433684 | 4.3:+22338442 | MS.gene28698:CDS |
| AGACCTCAGTGTTACTACTA+TGG | 0.457876 | 4.3:+22338688 | MS.gene28698:CDS |
| GAATGATTTGAACATTGAAA+TGG | 0.483853 | 4.3:+22338664 | MS.gene28698:CDS |
| CACAATAGAGCTTGATGGAA+TGG | 0.493719 | 4.3:+22338369 | MS.gene28698:CDS |
| AGTCTCACAATAGAGCTTGA+TGG | 0.495480 | 4.3:+22338364 | MS.gene28698:CDS |
| GTGCAGGTAGCAAAAGTTGA+TGG | 0.518362 | 4.3:+22338572 | MS.gene28698:CDS |
| ATGCTCATTGAGAAGAAAAG+AGG | 0.521277 | 4.3:+22338614 | MS.gene28698:CDS |
| ATGTGTGTCTATGGAAGAGT+TGG | 0.537161 | 4.3:+22337685 | MS.gene28698:CDS |
| GAACAAAGCAAGCATTATTG+AGG | 0.548126 | 4.3:+22338300 | MS.gene28698:CDS |
| CTTTCTATAGGAAGAGGTGC+AGG | 0.549579 | 4.3:+22338556 | MS.gene28698:intron |
| CTCAGTGTTACTACTATGGC+AGG | 0.550252 | 4.3:+22338692 | MS.gene28698:CDS |
| GCTGAAAATGGTAGCAATAG+GGG | 0.556414 | 4.3:+22337716 | MS.gene28698:CDS |
| GGAATTCGATAAAGTTCAAG+TGG | 0.574985 | 4.3:-22338939 | None:intergenic |
| AATTTATAGTGTCACCTGCA+AGG | 0.580808 | 4.3:-22338735 | None:intergenic |
| AAGTTGATGGAAATAAGCTA+TGG | 0.582461 | 4.3:+22338585 | MS.gene28698:CDS |
| TTGAGACAGAAAGAAAGAGA+AGG | 0.583322 | 4.3:+22337804 | MS.gene28698:CDS |
| AACAAGCACCGTGATAATGA+TGG | 0.583729 | 4.3:+22337752 | MS.gene28698:CDS |
| TGTGTGTCTATGGAAGAGTT+GGG | 0.584878 | 4.3:+22337686 | MS.gene28698:CDS |
| TTGGGACACCATCATTATCA+CGG | 0.595132 | 4.3:-22337760 | None:intergenic |
| TGAGACAGAAAGAAAGAGAA+GGG | 0.595420 | 4.3:+22337805 | MS.gene28698:CDS |
| GCAATAGGGGAAACTGGAGA+AGG | 0.660867 | 4.3:+22337729 | MS.gene28698:CDS |
| TCATTGAGAAGAAAAGAGGA+AGG | 0.693261 | 4.3:+22338618 | MS.gene28698:CDS |
| TTGATGAGATGAATAAAATG+GGG | 0.713073 | 4.3:+22338443 | MS.gene28698:CDS |
| TTATAGTGTCACCTGCAAGG+AGG | 0.739971 | 4.3:-22338732 | None:intergenic |
| CTGCCATAGTAGTAACACTG+AGG | 0.765658 | 4.3:-22338691 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTATAATAATTAGGTCATA+CGG | - | chr4.3:22338138-22338157 | None:intergenic | 15.0% |
| !!! | AGTTTTTAATTAGTACATTA+TGG | + | chr4.3:22338243-22338262 | MS.gene28698:intron | 15.0% |
| !!! | ATTCTATTTTTGTTATTTTC+AGG | + | chr4.3:22338902-22338921 | MS.gene28698:intron | 15.0% |
| !! | ATATAGTTTAGAGGATTTAA+TGG | - | chr4.3:22337957-22337976 | None:intergenic | 20.0% |
| !! | GATAATGATATATAGTTTAG+AGG | - | chr4.3:22337966-22337985 | None:intergenic | 20.0% |
| !! | TTTCATGATTTATCATCTAT+TGG | + | chr4.3:22337919-22337938 | MS.gene28698:intron | 20.0% |
| !!! | ATATGTTCTTTTAGTAATGT+TGG | + | chr4.3:22338525-22338544 | MS.gene28698:intron | 20.0% |
| !!! | GTTCATCTAAAATCAAATTT+AGG | - | chr4.3:22338284-22338303 | None:intergenic | 20.0% |
| !!! | TATGTTCTTTTAGTAATGTT+GGG | + | chr4.3:22338526-22338545 | MS.gene28698:intron | 20.0% |
| ! | GAATGATTTGAACATTGAAA+TGG | + | chr4.3:22338664-22338683 | MS.gene28698:CDS | 25.0% |
| ! | TGAAACATTTCATAGACATT+AGG | - | chr4.3:22338031-22338050 | None:intergenic | 25.0% |
| ! | TTGATGAGATGAATAAAATG+GGG | + | chr4.3:22338443-22338462 | MS.gene28698:CDS | 25.0% |
| !! | ATGTTCTTTTAGTAATGTTG+GGG | + | chr4.3:22338527-22338546 | MS.gene28698:intron | 25.0% |
| !! | GTTGATGAGATGAATAAAAT+GGG | + | chr4.3:22338442-22338461 | MS.gene28698:CDS | 25.0% |
| !! | TGTTGATGAGATGAATAAAA+TGG | + | chr4.3:22338441-22338460 | MS.gene28698:CDS | 25.0% |
| !!! | TTTTGTTATTTTCAGGACAT+GGG | + | chr4.3:22338909-22338928 | MS.gene28698:intron | 25.0% |
| !!! | TTTTTGTTATTTTCAGGACA+TGG | + | chr4.3:22338908-22338927 | MS.gene28698:intron | 25.0% |
| GATGTTAGAGACTAAATTGA+CGG | - | chr4.3:22338080-22338099 | None:intergenic | 30.0% | |
| TATAGATCATGAGACTCATT+AGG | - | chr4.3:22338508-22338527 | None:intergenic | 30.0% | |
| TTGATACTTTCTATAGGAAG+AGG | + | chr4.3:22338550-22338569 | MS.gene28698:intron | 30.0% | |
| !! | AAGTTGATGGAAATAAGCTA+TGG | + | chr4.3:22338585-22338604 | MS.gene28698:CDS | 30.0% |
| AACTTCATGTTGCAACTTCA+TGG | - | chr4.3:22338341-22338360 | None:intergenic | 35.0% | |
| AATTTATAGTGTCACCTGCA+AGG | - | chr4.3:22338738-22338757 | None:intergenic | 35.0% | |
| ATGCTCATTGAGAAGAAAAG+AGG | + | chr4.3:22338614-22338633 | MS.gene28698:CDS | 35.0% | |
| ATGCTGAAAATGGTAGCAAT+AGG | + | chr4.3:22337714-22337733 | MS.gene28698:CDS | 35.0% | |
| CAATAGACTATGTGTGTCTA+TGG | + | chr4.3:22337676-22337695 | MS.gene28698:CDS | 35.0% | |
| GAACAAAGCAAGCATTATTG+AGG | + | chr4.3:22338300-22338319 | MS.gene28698:CDS | 35.0% | |
| GATGAATAAAATGGGGTTAC+AGG | + | chr4.3:22338450-22338469 | MS.gene28698:CDS | 35.0% | |
| GGAATTCGATAAAGTTCAAG+TGG | - | chr4.3:22338942-22338961 | None:intergenic | 35.0% | |
| GTGCTTACATTTGTGATGAT+TGG | - | chr4.3:22337872-22337891 | None:intergenic | 35.0% | |
| TCATTGAGAAGAAAAGAGGA+AGG | + | chr4.3:22338618-22338637 | MS.gene28698:CDS | 35.0% | |
| TGAGACAGAAAGAAAGAGAA+GGG | + | chr4.3:22337805-22337824 | MS.gene28698:CDS | 35.0% | |
| TGCTGAAAATGGTAGCAATA+GGG | + | chr4.3:22337715-22337734 | MS.gene28698:CDS | 35.0% | |
| TTCTCTTTCTTTCTGTCTCA+AGG | - | chr4.3:22337805-22337824 | None:intergenic | 35.0% | |
| TTGAGACAGAAAGAAAGAGA+AGG | + | chr4.3:22337804-22337823 | MS.gene28698:CDS | 35.0% | |
| TTGGGTTTCAATGCTGAAAA+TGG | + | chr4.3:22337704-22337723 | MS.gene28698:CDS | 35.0% | |
| ! | ACGGATTTTCGTACACTATA+TGG | - | chr4.3:22338061-22338080 | None:intergenic | 35.0% |
| ! | TCAACTCTTTTCTCTCTTCT+TGG | - | chr4.3:22338397-22338416 | None:intergenic | 35.0% |
| ! | TCTTGGATGTGAACACTTTT+GGG | - | chr4.3:22337781-22337800 | None:intergenic | 35.0% |
| ! | TTCTTGGATGTGAACACTTT+TGG | - | chr4.3:22337782-22337801 | None:intergenic | 35.0% |
| !! | TTGGGGTTGATACTTTCTAT+AGG | + | chr4.3:22338544-22338563 | MS.gene28698:intron | 35.0% |
| AGTCTCACAATAGAGCTTGA+TGG | + | chr4.3:22338364-22338383 | MS.gene28698:CDS | 40.0% | |
| ATGTGTGTCTATGGAAGAGT+TGG | + | chr4.3:22337685-22337704 | MS.gene28698:CDS | 40.0% | |
| CACAATAGAGCTTGATGGAA+TGG | + | chr4.3:22338369-22338388 | MS.gene28698:CDS | 40.0% | |
| GCTGAAAATGGTAGCAATAG+GGG | + | chr4.3:22337716-22337735 | MS.gene28698:CDS | 40.0% | |
| TCTTTCTGTCTCAAGGTTCT+TGG | - | chr4.3:22337798-22337817 | None:intergenic | 40.0% | |
| TGTGTGTCTATGGAAGAGTT+GGG | + | chr4.3:22337686-22337705 | MS.gene28698:CDS | 40.0% | |
| TTGGGACACCATCATTATCA+CGG | - | chr4.3:22337763-22337782 | None:intergenic | 40.0% | |
| ! | AACAAGCACCGTGATAATGA+TGG | + | chr4.3:22337752-22337771 | MS.gene28698:CDS | 40.0% |
| ! | AGACCTCAGTGTTACTACTA+TGG | + | chr4.3:22338688-22338707 | MS.gene28698:CDS | 40.0% |
| ! | GAAACTCACTAGCAGACTTT+TGG | + | chr4.3:22337829-22337848 | MS.gene28698:CDS | 40.0% |
| !! | GCAGAAGACTCTCTTTGTTT+TGG | - | chr4.3:22338421-22338440 | None:intergenic | 40.0% |
| !!! | TGTTATTTTCAGGACATGGG+TGG | + | chr4.3:22338912-22338931 | MS.gene28698:intron | 40.0% |
| ATGGTAGCAATAGGGGAAAC+TGG | + | chr4.3:22337723-22337742 | MS.gene28698:CDS | 45.0% | |
| CTGCCATAGTAGTAACACTG+AGG | - | chr4.3:22338694-22338713 | None:intergenic | 45.0% | |
| GTGCAGGTAGCAAAAGTTGA+TGG | + | chr4.3:22338572-22338591 | MS.gene28698:CDS | 45.0% | |
| TTATAGTGTCACCTGCAAGG+AGG | - | chr4.3:22338735-22338754 | None:intergenic | 45.0% | |
| ! | CTCAGTGTTACTACTATGGC+AGG | + | chr4.3:22338692-22338711 | MS.gene28698:CDS | 45.0% |
| ! | CTTTCTATAGGAAGAGGTGC+AGG | + | chr4.3:22338556-22338575 | MS.gene28698:intron | 45.0% |
| !!! | CTAGCAGACTTTTGGTGCTT+AGG | + | chr4.3:22337837-22337856 | MS.gene28698:CDS | 45.0% |
| !! | ACTAAATAATTATAATAATT+AGG | - | chr4.3:22338146-22338165 | None:intergenic | 5.0% |
| !!! | TATTATAATTATTTAGTTTA+TGG | + | chr4.3:22338149-22338168 | MS.gene28698:intron | 5.0% |
| GCAATAGGGGAAACTGGAGA+AGG | + | chr4.3:22337729-22337748 | MS.gene28698:CDS | 50.0% | |
| TCTCATTACAGCCTCCTTGC+AGG | + | chr4.3:22338721-22338740 | MS.gene28698:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 22337659 | 22338980 | 22337659 | ID=MS.gene28698 |
| chr4.3 | mRNA | 22337659 | 22338980 | 22337659 | ID=MS.gene28698.t1;Parent=MS.gene28698 |
| chr4.3 | exon | 22337659 | 22337883 | 22337659 | ID=MS.gene28698.t1.exon1;Parent=MS.gene28698.t1 |
| chr4.3 | CDS | 22337659 | 22337883 | 22337659 | ID=cds.MS.gene28698.t1;Parent=MS.gene28698.t1 |
| chr4.3 | exon | 22338298 | 22338471 | 22338298 | ID=MS.gene28698.t1.exon2;Parent=MS.gene28698.t1 |
| chr4.3 | CDS | 22338298 | 22338471 | 22338298 | ID=cds.MS.gene28698.t1;Parent=MS.gene28698.t1 |
| chr4.3 | exon | 22338566 | 22338742 | 22338566 | ID=MS.gene28698.t1.exon3;Parent=MS.gene28698.t1 |
| chr4.3 | CDS | 22338566 | 22338742 | 22338566 | ID=cds.MS.gene28698.t1;Parent=MS.gene28698.t1 |
| chr4.3 | exon | 22338924 | 22338980 | 22338924 | ID=MS.gene28698.t1.exon4;Parent=MS.gene28698.t1 |
| chr4.3 | CDS | 22338924 | 22338980 | 22338924 | ID=cds.MS.gene28698.t1;Parent=MS.gene28698.t1 |
| Gene Sequence |
| Protein sequence |
