AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene28919

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28919.t1 MTR_4g079500 98.366 306 2 1 1 306 26 328 0.0 615
MS.gene28919.t1 MTR_2g090900 63.810 315 80 13 6 306 29 323 8.22e-107 314
MS.gene28919.t1 MTR_2g090900 63.323 319 81 14 1 305 26 322 6.61e-106 312
MS.gene28919.t1 MTR_2g078370 43.860 171 74 10 18 178 56 214 1.23e-23 96.7
MS.gene28919.t1 MTR_8g088650 52.941 102 43 1 166 267 228 324 1.90e-23 99.4
MS.gene28919.t1 MTR_2g078360 60.000 75 20 2 226 291 1 74 2.61e-22 89.4
MS.gene28919.t1 MTR_5g075390 48.387 93 48 0 172 264 159 251 5.45e-20 89.4
MS.gene28919.t1 MTR_4g072090 37.589 141 65 2 149 266 314 454 5.98e-20 90.1
MS.gene28919.t1 MTR_2g099050 46.000 100 54 0 170 269 219 318 2.22e-19 88.2
MS.gene28919.t1 MTR_3g005230 49.425 87 44 0 181 267 136 222 3.61e-19 85.5
MS.gene28919.t1 MTR_6g034945 34.286 175 102 2 111 285 96 257 5.25e-19 85.9
MS.gene28919.t1 MTR_3g467120 45.161 93 51 0 172 264 177 269 3.64e-18 84.3
MS.gene28919.t1 MTR_3g094020 44.681 94 52 0 171 264 107 200 4.67e-17 80.5
MS.gene28919.t1 MTR_4g072090 35.714 126 58 2 149 251 314 439 2.83e-15 76.3
MS.gene28919.t1 MTR_1g023690 48.980 98 46 2 171 266 386 481 5.94e-13 69.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28919.t1 AT3G58120 56.592 311 90 10 19 306 41 329 1.33e-94 283
MS.gene28919.t1 AT2G42380 54.485 301 101 8 20 306 42 320 2.90e-90 272
MS.gene28919.t1 AT2G42380 50.498 301 102 9 20 306 42 309 5.75e-79 243
MS.gene28919.t1 AT3G58120 58.167 251 77 7 19 251 41 281 6.21e-78 239
MS.gene28919.t1 AT2G42380 55.417 240 83 5 20 251 42 265 1.03e-72 225
MS.gene28919.t1 AT2G42380 47.264 201 82 5 20 212 42 226 8.16e-43 147
MS.gene28919.t1 AT2G42380 47.264 201 82 5 20 212 42 226 8.16e-43 147
MS.gene28919.t1 AT1G58110 47.321 112 53 2 156 267 219 324 4.11e-22 95.5
MS.gene28919.t1 AT1G58110 47.321 112 53 2 156 267 219 324 4.11e-22 95.5
MS.gene28919.t1 AT1G43700 49.485 97 49 0 170 266 192 288 1.23e-20 90.9
MS.gene28919.t1 AT4G38900 47.368 95 50 0 170 264 392 486 7.87e-20 90.1
MS.gene28919.t1 AT4G38900 47.368 95 50 0 170 264 392 486 7.87e-20 90.1
MS.gene28919.t1 AT2G40620 45.263 95 52 0 170 264 146 240 4.71e-19 86.7
MS.gene28919.t1 AT1G06070 44.000 100 56 0 170 269 208 307 1.01e-18 86.3
MS.gene28919.t1 AT2G21230 45.361 97 53 0 170 266 368 464 1.96e-18 85.9
MS.gene28919.t1 AT2G21230 45.361 97 53 0 170 266 368 464 1.96e-18 85.9
MS.gene28919.t1 AT1G06850 47.312 93 49 0 172 264 148 240 2.71e-18 84.3
MS.gene28919.t1 AT2G31370 42.991 107 61 0 163 269 192 298 3.85e-18 84.3
MS.gene28919.t1 AT2G31370 42.991 107 61 0 163 269 192 298 6.41e-18 84.0
MS.gene28919.t1 AT2G31370 42.991 107 61 0 163 269 192 298 6.41e-18 84.0
MS.gene28919.t1 AT2G31370 42.991 107 61 0 163 269 192 298 6.41e-18 84.0
MS.gene28919.t1 AT2G31370 42.991 107 61 0 163 269 192 298 6.41e-18 84.0
MS.gene28919.t1 AT2G31370 42.991 107 61 0 163 269 192 298 6.41e-18 84.0
MS.gene28919.t1 AT2G31370 42.991 107 61 0 163 269 192 298 6.41e-18 84.0
MS.gene28919.t1 AT4G38900 44.554 101 50 1 170 264 392 492 1.40e-17 83.6
MS.gene28919.t1 AT5G04840 50.000 84 42 0 181 264 177 260 2.90e-17 80.9
MS.gene28919.t1 AT1G35490 41.509 106 56 2 160 264 158 258 7.48e-17 79.7
MS.gene28919.t1 AT1G35490 41.509 106 56 2 160 264 158 258 7.48e-17 79.7
MS.gene28919.t1 AT2G21230 41.748 103 54 1 170 266 368 470 4.62e-16 79.0
MS.gene28919.t1 AT2G21230 45.122 82 45 0 170 251 368 449 1.78e-14 73.9
MS.gene28919.t1 AT5G04840 51.389 72 35 0 181 252 177 248 8.30e-14 70.5
MS.gene28919.t1 AT5G07160 41.489 94 55 0 171 264 47 140 4.56e-12 63.5
MS.gene28919.t1 AT1G06850 70.455 44 13 0 172 215 148 191 4.05e-11 62.4
MS.gene28919.t1 AT2G12900 34.021 97 64 0 170 266 114 210 8.25e-11 62.0

Find 57 sgRNAs with CRISPR-Local

Find 102 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCACCACGGGTTGCATATT+TGG 0.213119 4.2:-30732452 MS.gene28919:CDS
ACGTCTGTGTGCCCCTCTTC+TGG 0.291136 4.2:+30733492 None:intergenic
TCATTATCACATCGTGGTTT+AGG 0.296604 4.2:+30731583 None:intergenic
GGTGAATGCAAACAGGAAAA+TGG 0.353861 4.2:-30733169 MS.gene28919:CDS
GAATCTGATGAGGATGAAAT+TGG 0.366529 4.2:-30733190 MS.gene28919:CDS
ACGAAGTGTGACTTCATTAC+AGG 0.377047 4.2:-30732645 MS.gene28919:intron
ACAATCAACAAAACCCTTCA+TGG 0.391610 4.2:-30733543 MS.gene28919:CDS
AATGATGAAGATGACAATAA+TGG 0.401593 4.2:-30733136 MS.gene28919:CDS
GAAAAGTTTGAAGCATTACT+TGG 0.449386 4.2:+30733568 None:intergenic
TGAAATTGGTGAATGCAAAC+AGG 0.473536 4.2:-30733176 MS.gene28919:CDS
AAGAGGGGCACACAGACGTT+CGG 0.489835 4.2:-30733488 MS.gene28919:CDS
GTAGCTGATGGTGCTGGTGG+TGG 0.496771 4.2:+30733319 None:intergenic
ATGGAGACAATGAGTTTGAA+AGG 0.498812 4.2:-30733408 MS.gene28919:CDS
TGAAGCATTACTTGGTGAGA+TGG 0.500952 4.2:+30733576 None:intergenic
TCACATCGTGGTTTAGGTGA+TGG 0.507950 4.2:+30731589 None:intergenic
TTATGATCAGAAGGAGTTGA+AGG 0.508247 4.2:+30733277 None:intergenic
CTGACCCCAAGAGGGTCAAA+AGG 0.522349 4.2:-30733081 MS.gene28919:intron
TGATGGAGCATTGTCGTCGT+AGG 0.526258 4.2:-30733432 MS.gene28919:CDS
TATGAAGGTCACTGAGTCAC+TGG 0.527916 4.2:+30733465 None:intergenic
CAAGACAAAATCTTCAAAGA+TGG 0.531158 4.2:-30732364 MS.gene28919:intron
CATCATCGATGCTTCTATGA+AGG 0.532117 4.2:+30733450 None:intergenic
TGGTGCTGGTGGTGGCATCA+TGG 0.538097 4.2:+30733327 None:intergenic
CTTCATAGAAGCATCGATGA+TGG 0.538192 4.2:-30733449 MS.gene28919:CDS
AGAAACTCGTCCACCCATGA+AGG 0.540302 4.2:+30733529 None:intergenic
GACTTCTCGTCCACCAGAAG+AGG 0.550382 4.2:-30733505 MS.gene28919:CDS
AGAGGGAGATAGAGAGGCTG+AGG 0.553494 4.2:-30731675 MS.gene28919:CDS
GAGCATTGTCGTCGTAGGGA+TGG 0.556038 4.2:-30733427 MS.gene28919:CDS
ACTTCTCGTCCACCAGAAGA+GGG 0.556593 4.2:-30733504 MS.gene28919:CDS
AGTATCAGTGCTGTCACCAC+GGG 0.561461 4.2:-30732464 MS.gene28919:CDS
CAATCAACAAAACCCTTCAT+GGG 0.563412 4.2:-30733542 MS.gene28919:CDS
TGATGACAAAGAGGAAGATG+AGG 0.565693 4.2:-30733245 MS.gene28919:CDS
TGACAAAGAGGAAGATGAGG+AGG 0.566303 4.2:-30733242 MS.gene28919:CDS
TTAGTGTAGACGAGGTTTCG+AGG 0.573188 4.2:-30733357 MS.gene28919:CDS
CATTGAAGAGGGAGATAGAG+AGG 0.587016 4.2:-30731681 MS.gene28919:CDS
TTGATGCTGTTATGATCAGA+AGG 0.588109 4.2:+30733268 None:intergenic
TTGCATATTTGGATCATCAG+AGG 0.590830 4.2:-30732441 MS.gene28919:CDS
TATGATCAGAAGGAGTTGAA+GGG 0.593074 4.2:+30733278 None:intergenic
CTCATCAAGAAGCATTGAAG+AGG 0.593087 4.2:-30731693 MS.gene28919:CDS
TCTGTGTGCCCCTCTTCTGG+TGG 0.594474 4.2:+30733495 None:intergenic
GACAATGTAGCTGATGGTGC+TGG 0.596193 4.2:+30733313 None:intergenic
GTCCATGTTTAGTGTAGACG+AGG 0.601046 4.2:-30733365 MS.gene28919:CDS
CAGCATCAATGATGACAAAG+AGG 0.602367 4.2:-30733254 MS.gene28919:CDS
GATGGAGCATTGTCGTCGTA+GGG 0.605532 4.2:-30733431 MS.gene28919:CDS
TCAACAAAACCCTTCATGGG+TGG 0.609845 4.2:-30733539 MS.gene28919:CDS
AACCTCGTCTACACTAAACA+TGG 0.622608 4.2:+30733363 None:intergenic
AATGTAGCTGATGGTGCTGG+TGG 0.623330 4.2:+30733316 None:intergenic
AAGTATCAGTGCTGTCACCA+CGG 0.625333 4.2:-30732465 MS.gene28919:CDS
TCATCAAGAAGCATTGAAGA+GGG 0.631177 4.2:-30731692 MS.gene28919:CDS
GAAACTCGTCCACCCATGAA+GGG 0.631276 4.2:+30733530 None:intergenic
CTGCACAAAGATCAAGAGTG+AGG 0.635769 4.2:-30732694 MS.gene28919:CDS
ATTGAAGCATGAATCTGATG+AGG 0.673573 4.2:-30733200 MS.gene28919:CDS
GAATGCAAACAGGAAAATGG+AGG 0.685882 4.2:-30733166 MS.gene28919:CDS
CTTCTCGTCCACCAGAAGAG+GGG 0.695347 4.2:-30733503 MS.gene28919:CDS
CGTGGTTTAGGTGATGGAGA+TGG 0.696148 4.2:+30731595 None:intergenic
TGAGTATCATTATCACATCG+TGG 0.696934 4.2:+30731577 None:intergenic
TTAGATGACAATGTAGCTGA+TGG 0.701045 4.2:+30733307 None:intergenic
TGATCCAAATATGCAACCCG+TGG 0.752305 4.2:+30732448 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAATAATTAAACAATTTTG+AGG + chr4.2:30733048-30733067 None:intergenic 10.0%
!!! TGTTTAATTATTTTTACTAT+AGG - chr4.2:30733054-30733073 MS.gene28919:intron 10.0%
!! GAATGAAAAAACTATATAAT+AGG - chr4.2:30733103-30733122 MS.gene28919:CDS 15.0%
!!! ATCTATAAGTATTATTTTGA+AGG + chr4.2:30733380-30733399 None:intergenic 15.0%
!!! ATTGATAATACTACAAATTA+AGG + chr4.2:30732156-30732175 None:intergenic 15.0%
!! CTATATAATAGGCTTATAAA+TGG - chr4.2:30733114-30733133 MS.gene28919:CDS 20.0%
!!! TAATACACATATTTGGTTTT+AGG + chr4.2:30732125-30732144 None:intergenic 20.0%
!!! TATAATGTATTGATGAATGA+AGG - chr4.2:30732647-30732666 MS.gene28919:CDS 20.0%
!!! TATATATTGTTATTGATACG+TGG - chr4.2:30732898-30732917 MS.gene28919:intron 20.0%
! AACCAAATATGTGTATTACA+TGG - chr4.2:30732127-30732146 MS.gene28919:intron 25.0%
! AATGATGAAGATGACAATAA+TGG - chr4.2:30732002-30732021 MS.gene28919:intron 25.0%
! GAAAGTTTAGACAGAATTAT+AGG - chr4.2:30732951-30732970 MS.gene28919:intron 25.0%
!! TTATTAATTCTAGCTAGCTA+TGG - chr4.2:30732210-30732229 MS.gene28919:intron 25.0%
!!! ATCTTTGAAGATTTTGTCTT+GGG + chr4.2:30732775-30732794 None:intergenic 25.0%
AATGGAACAGAGGATAATAA+CGG - chr4.2:30733132-30733151 MS.gene28919:CDS 30.0%
AGCCATGTAATACACATATT+TGG + chr4.2:30732132-30732151 None:intergenic 30.0%
ATACTACATGACAGTGTAAA+AGG + chr4.2:30732831-30732850 None:intergenic 30.0%
CAAGACAAAATCTTCAAAGA+TGG - chr4.2:30732774-30732793 MS.gene28919:intron 30.0%
GGTGAAATGAGAATATGTTA+AGG + chr4.2:30732810-30732829 None:intergenic 30.0%
GTCATTAAGATTACTACTAG+TGG - chr4.2:30733341-30733360 MS.gene28919:CDS 30.0%
TTACATTGAACATGACATGA+TGG - chr4.2:30732530-30732549 MS.gene28919:intron 30.0%
! GAAAAGTTTGAAGCATTACT+TGG + chr4.2:30731573-30731592 None:intergenic 30.0%
!! CATCTTTGAAGATTTTGTCT+TGG + chr4.2:30732776-30732795 None:intergenic 30.0%
!! TGTTCTTGATGTTTTGTTGT+TGG + chr4.2:30733500-30733519 None:intergenic 30.0%
AAAAATTACTGACCCCAAGA+GGG - chr4.2:30732049-30732068 MS.gene28919:intron 35.0%
ACAATCAACAAAACCCTTCA+TGG - chr4.2:30731595-30731614 MS.gene28919:CDS 35.0%
ATGGAGACAATGAGTTTGAA+AGG - chr4.2:30731730-30731749 MS.gene28919:intron 35.0%
ATTGAAGCATGAATCTGATG+AGG - chr4.2:30731938-30731957 MS.gene28919:intron 35.0%
CAATCAACAAAACCCTTCAT+GGG - chr4.2:30731596-30731615 MS.gene28919:CDS 35.0%
CAGAGCAATTATTAGAGAAG+AGG - chr4.2:30733206-30733225 MS.gene28919:CDS 35.0%
GAATCTGATGAGGATGAAAT+TGG - chr4.2:30731948-30731967 MS.gene28919:intron 35.0%
TAGGCTTATAAATGGAACAG+AGG - chr4.2:30733122-30733141 MS.gene28919:CDS 35.0%
TATGATCAGAAGGAGTTGAA+GGG + chr4.2:30731863-30731882 None:intergenic 35.0%
TCACCATCACATGAATGAAA+AGG + chr4.2:30732374-30732393 None:intergenic 35.0%
TCATTATCACATCGTGGTTT+AGG + chr4.2:30733558-30733577 None:intergenic 35.0%
TGAAATTGGTGAATGCAAAC+AGG - chr4.2:30731962-30731981 MS.gene28919:intron 35.0%
TGAGTATCATTATCACATCG+TGG + chr4.2:30733564-30733583 None:intergenic 35.0%
TTAGATGACAATGTAGCTGA+TGG + chr4.2:30731834-30731853 None:intergenic 35.0%
TTATGATCAGAAGGAGTTGA+AGG + chr4.2:30731864-30731883 None:intergenic 35.0%
TTGATGCTGTTATGATCAGA+AGG + chr4.2:30731873-30731892 None:intergenic 35.0%
TTGCATATTTGGATCATCAG+AGG - chr4.2:30732697-30732716 MS.gene28919:CDS 35.0%
! GTTCCTTTTCATTCATGTGA+TGG - chr4.2:30732368-30732387 MS.gene28919:CDS 35.0%
!! TACTTGGTGAGATGGTTTTA+AGG + chr4.2:30731557-30731576 None:intergenic 35.0%
!! TCATCAAGAAGCATTGAAGA+GGG - chr4.2:30733446-30733465 MS.gene28919:CDS 35.0%
!!! CGGAATTAGCTTCTTTTTTG+TGG - chr4.2:30733152-30733171 MS.gene28919:CDS 35.0%
!!! TGAAGGGTTTTGTTGATTGT+TGG + chr4.2:30731595-30731614 None:intergenic 35.0%
AACCTCGTCTACACTAAACA+TGG + chr4.2:30731778-30731797 None:intergenic 40.0%
ACGAAGTGTGACTTCATTAC+AGG - chr4.2:30732493-30732512 MS.gene28919:intron 40.0%
ATTGAAACATGAGCTAGCAG+TGG - chr4.2:30732316-30732335 MS.gene28919:intron 40.0%
CAAAAATTACTGACCCCAAG+AGG - chr4.2:30732048-30732067 MS.gene28919:intron 40.0%
CAGCATCAATGATGACAAAG+AGG - chr4.2:30731884-30731903 MS.gene28919:intron 40.0%
CATCATCGATGCTTCTATGA+AGG + chr4.2:30731691-30731710 None:intergenic 40.0%
GAATGCAAACAGGAAAATGG+AGG - chr4.2:30731972-30731991 MS.gene28919:intron 40.0%
GCAATTGCAAGCTTAGTTAG+TGG + chr4.2:30733021-30733040 None:intergenic 40.0%
GGTGAATGCAAACAGGAAAA+TGG - chr4.2:30731969-30731988 MS.gene28919:intron 40.0%
TGATGACAAAGAGGAAGATG+AGG - chr4.2:30731893-30731912 MS.gene28919:intron 40.0%
TTGTGGAACTTTGCATCGTA+CGG - chr4.2:30733169-30733188 MS.gene28919:CDS 40.0%
! CTCTTGGGGTCAGTAATTTT+TGG + chr4.2:30732050-30732069 None:intergenic 40.0%
! GAAGATTTTGTCTTGGGCTA+GGG + chr4.2:30732769-30732788 None:intergenic 40.0%
! TAGTACCTTTTGACCCTCTT+GGG + chr4.2:30732065-30732084 None:intergenic 40.0%
! TGAAGATTTTGTCTTGGGCT+AGG + chr4.2:30732770-30732789 None:intergenic 40.0%
! TGAAGCATTACTTGGTGAGA+TGG + chr4.2:30731565-30731584 None:intergenic 40.0%
!! CTCATCAAGAAGCATTGAAG+AGG - chr4.2:30733445-30733464 MS.gene28919:CDS 40.0%
!! CTTCATAGAAGCATCGATGA+TGG - chr4.2:30731689-30731708 MS.gene28919:CDS 40.0%
!!! GGTGAGATGGTTTTAAGGTT+AGG + chr4.2:30731552-30731571 None:intergenic 40.0%
AAGTATCAGTGCTGTCACCA+CGG - chr4.2:30732673-30732692 MS.gene28919:CDS 45.0%
CATTGAAGAGGGAGATAGAG+AGG - chr4.2:30733457-30733476 MS.gene28919:CDS 45.0%
CTGCACAAAGATCAAGAGTG+AGG - chr4.2:30732444-30732463 MS.gene28919:CDS 45.0%
GTCCATGTTTAGTGTAGACG+AGG - chr4.2:30731773-30731792 MS.gene28919:intron 45.0%
TATGAAGGTCACTGAGTCAC+TGG + chr4.2:30731676-30731695 None:intergenic 45.0%
TCAACAAAACCCTTCATGGG+TGG - chr4.2:30731599-30731618 MS.gene28919:CDS 45.0%
TCACATCGTGGTTTAGGTGA+TGG + chr4.2:30733552-30733571 None:intergenic 45.0%
TGACAAAGAGGAAGATGAGG+AGG - chr4.2:30731896-30731915 MS.gene28919:intron 45.0%
TGATCCAAATATGCAACCCG+TGG + chr4.2:30732693-30732712 None:intergenic 45.0%
TTAGTGTAGACGAGGTTTCG+AGG - chr4.2:30731781-30731800 MS.gene28919:intron 45.0%
! AGTACCTTTTGACCCTCTTG+GGG + chr4.2:30732064-30732083 None:intergenic 45.0%
! GTAGTACCTTTTGACCCTCT+TGG + chr4.2:30732066-30732085 None:intergenic 45.0%
!! GGGGTCAGTAATTTTTGGAC+TGG + chr4.2:30732045-30732064 None:intergenic 45.0%
!!! AATTTAAAATAAATAAGTAA+AGG + chr4.2:30732275-30732294 None:intergenic 5.0%
!!! ATTTATTTTAAATTTTTTTC+TGG - chr4.2:30732281-30732300 MS.gene28919:intron 5.0%
ACATGAGCTAGCAGTGGTGA+TGG - chr4.2:30732322-30732341 MS.gene28919:intron 50.0%
ACTTCTCGTCCACCAGAAGA+GGG - chr4.2:30731634-30731653 MS.gene28919:CDS 50.0%
AGAAACTCGTCCACCCATGA+AGG + chr4.2:30731612-30731631 None:intergenic 50.0%
AGTATCAGTGCTGTCACCAC+GGG - chr4.2:30732674-30732693 MS.gene28919:CDS 50.0%
CTAGTTGCAGACACAGCACA+TGG + chr4.2:30733313-30733332 None:intergenic 50.0%
GAAACTCGTCCACCCATGAA+GGG + chr4.2:30731611-30731630 None:intergenic 50.0%
! AATGTAGCTGATGGTGCTGG+TGG + chr4.2:30731825-30731844 None:intergenic 50.0%
! GACAATGTAGCTGATGGTGC+TGG + chr4.2:30731828-30731847 None:intergenic 50.0%
! GTCACCACGGGTTGCATATT+TGG - chr4.2:30732686-30732705 MS.gene28919:CDS 50.0%
!! CGTGGTTTAGGTGATGGAGA+TGG + chr4.2:30733546-30733565 None:intergenic 50.0%
!! GATGGAGCATTGTCGTCGTA+GGG - chr4.2:30731707-30731726 MS.gene28919:intron 50.0%
!! TGATGGAGCATTGTCGTCGT+AGG - chr4.2:30731706-30731725 MS.gene28919:intron 50.0%
AGAGGGAGATAGAGAGGCTG+AGG - chr4.2:30733463-30733482 MS.gene28919:CDS 55.0%
CTGACCCCAAGAGGGTCAAA+AGG - chr4.2:30732057-30732076 MS.gene28919:intron 55.0%
CTTCTCGTCCACCAGAAGAG+GGG - chr4.2:30731635-30731654 MS.gene28919:CDS 55.0%
GACTTCTCGTCCACCAGAAG+AGG - chr4.2:30731633-30731652 MS.gene28919:CDS 55.0%
! AAGAGGGGCACACAGACGTT+CGG - chr4.2:30731650-30731669 MS.gene28919:CDS 55.0%
! GAGCATTGTCGTCGTAGGGA+TGG - chr4.2:30731711-30731730 MS.gene28919:intron 55.0%
ACGTCTGTGTGCCCCTCTTC+TGG + chr4.2:30731649-30731668 None:intergenic 60.0%
TCTGTGTGCCCCTCTTCTGG+TGG + chr4.2:30731646-30731665 None:intergenic 60.0%
!! GTAGCTGATGGTGCTGGTGG+TGG + chr4.2:30731822-30731841 None:intergenic 60.0%
!! TGGTGCTGGTGGTGGCATCA+TGG + chr4.2:30731814-30731833 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 30731546 30733614 30731546 ID=MS.gene28919
chr4.2 mRNA 30731546 30733614 30731546 ID=MS.gene28919.t1;Parent=MS.gene28919
chr4.2 exon 30733082 30733614 30733082 ID=MS.gene28919.t1.exon1;Parent=MS.gene28919.t1
chr4.2 CDS 30733082 30733614 30733082 ID=cds.MS.gene28919.t1;Parent=MS.gene28919.t1
chr4.2 exon 30732646 30732736 30732646 ID=MS.gene28919.t1.exon2;Parent=MS.gene28919.t1
chr4.2 CDS 30732646 30732736 30732646 ID=cds.MS.gene28919.t1;Parent=MS.gene28919.t1
chr4.2 exon 30732365 30732491 30732365 ID=MS.gene28919.t1.exon3;Parent=MS.gene28919.t1
chr4.2 CDS 30732365 30732491 30732365 ID=cds.MS.gene28919.t1;Parent=MS.gene28919.t1
chr4.2 exon 30731546 30731715 30731546 ID=MS.gene28919.t1.exon4;Parent=MS.gene28919.t1
chr4.2 CDS 30731546 30731715 30731546 ID=cds.MS.gene28919.t1;Parent=MS.gene28919.t1
Gene Sequence

>MS.gene28919

ATGCCTAACCTTAAAACCATCTCACCAAGTAATGCTTCAAACTTTTCCAACAATCAACAAAACCCTTCATGGGTGGACGAGTTTCTTGACTTCTCGTCCACCAGAAGAGGGGCACACAGACGTTCGGCCAGTGACTCAGTGACCTTCATAGAAGCATCGATGATGGAGCATTGTCGTCGTAGGGATGGAGACAATGAGTTTGAAAGGTTTGATGATGAACAATTGATGTCCATGTTTAGTGTAGACGAGGTTTCGAGGAATAATACCATGATGCCACCACCAGCACCATCAGCTACATTGTCATCTAACTCTAACCCTTCAACTCCTTCTGATCATAACAGCATCAATGATGACAAAGAGGAAGATGAGGAGGAAAAGATGCAACAGAAACAATTGAAGCATGAATCTGATGAGGATGAAATTGGTGAATGCAAACAGGAAAATGGAGGAGTTGTGAATGATGAAGATGACAATAATGGAAATACAACAACTTTTTCCAGTCCAAAAATTACTGACCCCAAGAGGGTCAAAAGGTACTACTCAAACATATTATTCATGTCACACAATTGTCACATTCCTAAAACCAAATATGTGTATTACATGGCTTCCTTAATTTGTAGTATTATCAATGTGTGAGCGAGACAATTTAATGTTAATTTGTGTATTATTAATTCTAGCTAGCTATGGAGAACATGTTCTTCATATATAAATATATACATCAGAAGACCTTTACTTATTTATTTTAAATTTTTTTCTGGTTTTATTTTCTGATTGAAACATGAGCTAGCAGTGGTGATGGTGATTATGTAAGAATATATTTCTGTTCCTTTTCATTCATGTGATGGTGATTGTAATTACTGTGTTTGCATATGTATCAGAATCTTAGCAAATAGACAATCTGCACAAAGATCAAGAGTGAGGAAACTGCAATACATATCAGAGCTTGAACGAAGTGTGACTTCATTACAGGTGAATTTAATAAACTTACATTGAACATGACATGATGGTAACTTAATTTTATACTAAGCATATACTATTAATAATTTGATGTGTATAGTAATTAATAATAATATTATGATTATATTTTGTTTTAACTTATTATATAATGTATTGATGAATGAAGGCTGAAGTATCAGTGCTGTCACCACGGGTTGCATATTTGGATCATCAGAGGTTGCTTCTAAATGTTGATAACAGTGCTATCAAGCAAAGAATCGCAGCCCTAGCCCAAGACAAAATCTTCAAAGATGGTATGCTACATCCTTAACATATTCTCATTTCACCTTTTACACTGTCATGTAGTATAAGTTTTCTGATTCATTAATTAATCACTGTTAAGATTTTGTTCTGTATATATATTGTTATTGATACGTGGTTTTATACTTTCAGTATGTCATGTTCAAGAGAAAGTTTAGACAGAATTATAGGAAGAAAGTACAATTTAGTTTATAGAGATGTACTTTTCAATTGTACCACTAACTAAGCTTGCAATTGCATCTCCTCAAAATTGTTTAATTATTTTTACTATAGGAGAGTTATTAAATATTAATTATTAATGAATGAAAAAACTATATAATAGGCTTATAAATGGAACAGAGGATAATAACGGAATTAGCTTCTTTTTTGTGGAACTTTGCATCGTACGGCACATGAGTTGAATCAGAGCAATTATTAGAGAAGAGGACAGATTGATATTGATTTTAGTAAAAGCACTTGCATGCAGTTGCACTGCACACATATTAAAATGCTGTATGTCTGAGATAACCATGTGCTGTGTCTGCAACTAGTAGTTATAGTCATTAAGATTACTACTAGTGGTACTACTTAAAAACCTTCAAAATAATACTTATAGATTGATTATTAATATTATACATGTTACAATGAATATTAATAATGCAGCTCATCAAGAAGCATTGAAGAGGGAGATAGAGAGGCTGAGGCAAGTGTATCACCAACAACAAAACATCAAGAACAATGCTACAGCAGCAGTAGTGTCACCATCTCCATCACCTAAACCACGATGTGATAATGATACTCACATTGAAAATGAACATCTTATCAATGTTTGA
Protein sequence

>MS.gene28919.t1

MPNLKTISPSNASNFSNNQQNPSWVDEFLDFSSTRRGAHRRSASDSVTFIEASMMEHCRRRDGDNEFERFDDEQLMSMFSVDEVSRNNTMMPPPAPSATLSSNSNPSTPSDHNSINDDKEEDEEEKMQQKQLKHESDEDEIGECKQENGGVVNDEDDNNGNTTTFSSPKITDPKRVKRILANRQSAQRSRVRKLQYISELERSVTSLQAEVSVLSPRVAYLDHQRLLLNVDNSAIKQRIAALAQDKIFKDAHQEALKREIERLRQVYHQQQNIKNNATAAVVSPSPSPKPRCDNDTHIENEHLINV