AlfalfaGEDB | MS.gene29017

M. sativa cultivar XinJiangDaYe / MS.gene29017

KEGG: -
GO: -
PFAM: PF03195.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene29017.t1	RHN68580.1	93.6	203	9	1	1	203	1	199	2.80E-99	371.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene29017.t1	Q9FKZ3	77.4	115	26	0	6	120	4	118	1.1e-46	188.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene29017.t1	A0A072UZB6	93.6	203	9	1	1	203	1	199	2.1e-99	371.3

TFs/TRs:

Gene ID	Type	Classification
MS.gene29017.t1	TF	LOB

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene29017.t1	MTR_3g073690	96.059	203	4	1	1	203	1	199	6.06e-144	399
MS.gene29017.t1	MTR_5g017950	77.236	123	28	0	2	124	1	123	3.39e-67	209
MS.gene29017.t1	MTR_3g452660	74.576	118	28	2	2	118	1	117	8.12e-57	181
MS.gene29017.t1	MTR_4g105170	66.957	115	38	0	8	122	10	124	2.03e-50	162
MS.gene29017.t1	MTR_7g033800	66.667	111	37	0	3	113	5	115	1.52e-49	159
MS.gene29017.t1	MTR_6g005070	66.972	109	36	0	1	109	1	109	1.00e-48	156
MS.gene29017.t1	MTR_3g077240	65.217	115	40	0	3	117	4	118	1.03e-48	157
MS.gene29017.t1	MTR_6g005080	63.393	112	41	0	1	112	2	113	6.41e-48	154
MS.gene29017.t1	MTR_5g083230	60.684	117	45	1	7	122	4	120	5.39e-47	152
MS.gene29017.t1	MTR_3g071590	61.111	108	42	0	2	109	1	108	1.22e-45	149
MS.gene29017.t1	MTR_3g031660	60.577	104	41	0	6	109	4	107	8.51e-45	147
MS.gene29017.t1	MTR_8g040900	58.394	137	54	2	2	138	1	134	1.35e-44	148
MS.gene29017.t1	MTR_5g083960	60.377	106	42	0	7	112	37	142	4.53e-43	143
MS.gene29017.t1	MTR_4g083680	60.784	102	40	0	7	108	13	114	9.14e-43	142
MS.gene29017.t1	MTR_2g093310	59.804	102	41	0	8	109	32	133	1.88e-42	142
MS.gene29017.t1	MTR_2g068760	60.784	102	40	0	8	109	12	113	5.24e-42	139
MS.gene29017.t1	MTR_3g094690	60.000	105	42	0	8	112	46	150	1.16e-41	141
MS.gene29017.t1	MTR_3g071420	62.745	102	38	0	6	107	11	112	5.03e-41	137
MS.gene29017.t1	MTR_8g079660	56.364	110	48	0	9	118	8	117	1.09e-40	139
MS.gene29017.t1	MTR_5g083010	60.952	105	41	0	6	110	8	112	3.33e-40	135
MS.gene29017.t1	MTR_3g094690	59.048	105	42	1	8	112	46	149	1.83e-39	135
MS.gene29017.t1	MTR_1g095850	57.426	101	43	0	10	110	73	173	3.92e-39	134
MS.gene29017.t1	MTR_8g079620	53.636	110	51	0	9	118	8	117	6.11e-39	134
MS.gene29017.t1	MTR_4g060950	56.311	103	44	1	8	109	23	125	6.23e-39	132
MS.gene29017.t1	MTR_5g075020	54.918	122	47	1	8	121	42	163	5.70e-38	131
MS.gene29017.t1	MTR_5g075020	54.918	122	47	1	8	121	46	167	9.32e-38	130
MS.gene29017.t1	MTR_8g079580	53.153	111	51	1	9	118	8	118	9.96e-36	125
MS.gene29017.t1	MTR_7g096530	55.046	109	48	1	3	110	8	116	1.30e-34	122
MS.gene29017.t1	MTR_7g028905	47.619	105	55	0	8	112	4	108	1.57e-34	119
MS.gene29017.t1	MTR_8g036085	55.660	106	46	1	8	112	22	127	2.27e-34	122
MS.gene29017.t1	MTR_7g074990	53.922	102	46	1	9	109	20	121	2.29e-33	119
MS.gene29017.t1	MTR_8g036105	41.989	181	98	2	6	185	15	189	3.26e-33	119
MS.gene29017.t1	MTR_1g070205	42.405	158	73	4	9	152	12	165	5.94e-33	118
MS.gene29017.t1	MTR_5g093010	46.602	103	55	0	10	112	6	108	1.47e-32	115
MS.gene29017.t1	MTR_1g070220	54.455	101	45	1	9	108	15	115	1.79e-32	116
MS.gene29017.t1	MTR_6g027710	50.485	103	50	1	8	109	39	141	2.07e-31	114
MS.gene29017.t1	MTR_7g096610	49.091	110	55	1	2	110	1	110	7.02e-31	112
MS.gene29017.t1	MTR_6g011200	36.757	185	97	6	10	189	10	179	5.79e-30	111
MS.gene29017.t1	MTR_5g080470	46.667	105	55	1	7	110	5	109	5.93e-30	109
MS.gene29017.t1	MTR_7g075230	41.667	168	82	5	6	166	9	167	2.10e-29	108
MS.gene29017.t1	MTR_6g011230	46.000	100	54	0	7	106	9	108	4.00e-28	105
MS.gene29017.t1	MTR_6g027700	46.602	103	54	1	7	108	5	107	1.98e-27	103
MS.gene29017.t1	MTR_6g011250	46.078	102	54	1	10	110	10	111	8.70e-27	101
MS.gene29017.t1	MTR_4g107450	45.000	100	55	0	10	109	11	110	4.37e-26	102
MS.gene29017.t1	MTR_4g099230	40.476	126	70	3	10	134	3	124	3.31e-25	96.3
MS.gene29017.t1	MTR_8g017090	38.835	103	63	0	10	112	13	115	3.22e-24	97.4
MS.gene29017.t1	MTR_5g085390	42.157	102	58	1	10	110	10	111	7.26e-22	89.7
MS.gene29017.t1	MTR_2g100020	36.634	101	64	0	10	110	20	120	3.12e-21	89.0
MS.gene29017.t1	MTR_4g088035	37.778	90	56	0	6	95	3	92	9.18e-20	84.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene29017.t1	AT5G66870	78.070	114	25	0	7	120	5	118	1.04e-61	194
MS.gene29017.t1	AT2G23660	66.379	116	39	0	7	122	3	118	8.42e-56	179
MS.gene29017.t1	AT2G23660	66.379	116	39	0	7	122	3	118	8.42e-56	179
MS.gene29017.t1	AT2G23660	66.379	116	39	0	7	122	3	118	8.42e-56	179
MS.gene29017.t1	AT5G63090	69.298	114	33	1	1	112	1	114	4.91e-51	163
MS.gene29017.t1	AT5G63090	69.298	114	33	1	1	112	1	114	4.91e-51	163
MS.gene29017.t1	AT5G63090	69.298	114	33	1	1	112	1	114	4.91e-51	163
MS.gene29017.t1	AT5G63090	69.298	114	33	1	1	112	1	114	4.91e-51	163
MS.gene29017.t1	AT3G27650	64.602	113	40	0	2	114	32	144	5.57e-49	157
MS.gene29017.t1	AT1G65620	66.942	121	40	0	1	121	1	121	6.06e-48	155
MS.gene29017.t1	AT1G65620	66.942	121	40	0	1	121	1	121	6.06e-48	155
MS.gene29017.t1	AT1G65620	66.942	121	40	0	1	121	1	121	6.06e-48	155
MS.gene29017.t1	AT1G65620	66.942	121	40	0	1	121	1	121	6.06e-48	155
MS.gene29017.t1	AT1G65620	66.942	121	40	0	1	121	1	121	6.06e-48	155
MS.gene29017.t1	AT2G30130	62.963	108	40	0	2	109	1	108	1.08e-47	155
MS.gene29017.t1	AT1G07900	60.194	103	41	0	8	110	32	134	1.91e-43	144
MS.gene29017.t1	AT2G28500	46.452	155	78	2	8	162	54	203	1.57e-42	143
MS.gene29017.t1	AT2G30340	59.804	102	41	0	8	109	51	152	6.12e-41	140
MS.gene29017.t1	AT2G30340	59.804	102	41	0	8	109	52	153	6.92e-41	140
MS.gene29017.t1	AT1G16530	59.223	103	41	1	8	109	13	115	2.86e-40	135
MS.gene29017.t1	AT3G11090	57.798	109	46	0	6	114	8	116	3.33e-40	135
MS.gene29017.t1	AT1G31320	62.921	89	33	0	7	95	11	99	7.96e-39	132
MS.gene29017.t1	AT2G40470	60.000	105	42	0	8	112	19	123	1.01e-37	130
MS.gene29017.t1	AT4G00210	56.410	117	46	2	7	122	9	121	1.32e-37	130
MS.gene29017.t1	AT2G40470	60.000	105	42	0	8	112	44	148	2.02e-37	129
MS.gene29017.t1	AT3G26660	50.000	106	53	0	6	111	2	107	3.11e-37	126
MS.gene29017.t1	AT3G26620	50.000	106	53	0	6	111	2	107	3.36e-37	126
MS.gene29017.t1	AT3G50510	49.580	119	59	1	7	124	10	128	1.44e-36	127
MS.gene29017.t1	AT3G50510	49.580	119	59	1	7	124	10	128	1.44e-36	127
MS.gene29017.t1	AT5G35900	50.459	109	54	0	6	114	2	110	4.82e-36	125
MS.gene29017.t1	AT3G03760	54.369	103	46	1	8	109	50	152	2.20e-34	123
MS.gene29017.t1	AT3G58190	44.286	140	73	3	9	146	11	147	4.48e-34	121
MS.gene29017.t1	AT4G00220	56.436	101	43	1	12	111	20	120	1.92e-32	117
MS.gene29017.t1	AT2G42430	52.336	107	50	1	7	112	13	119	7.88e-32	115
MS.gene29017.t1	AT2G45420	53.465	101	46	1	12	111	40	140	2.40e-30	112
MS.gene29017.t1	AT2G42440	47.573	103	53	1	7	108	5	107	8.28e-30	110
MS.gene29017.t1	AT2G45410	45.113	133	67	2	12	138	19	151	1.30e-29	108
MS.gene29017.t1	AT2G31310	46.602	103	54	1	7	108	5	107	1.05e-28	106
MS.gene29017.t1	AT5G06080	44.444	108	59	1	6	112	4	111	3.50e-27	102
MS.gene29017.t1	AT1G72980	43.925	107	60	0	3	109	7	113	5.83e-27	102
MS.gene29017.t1	AT3G13850	39.806	103	62	0	10	112	37	139	1.17e-26	103
MS.gene29017.t1	AT3G47870	40.566	106	63	0	7	112	34	139	3.15e-26	103
MS.gene29017.t1	AT4G22700	38.194	144	78	5	7	140	3	145	9.82e-24	93.6
MS.gene29017.t1	AT1G06280	45.977	87	47	0	10	96	25	111	1.42e-20	85.9
MS.gene29017.t1	AT2G19820	34.021	97	63	1	8	103	11	107	3.98e-16	72.0
MS.gene29017.t1	AT3G27940	30.769	130	88	1	6	133	2	131	2.31e-14	68.2
MS.gene29017.t1	AT3G27940	30.769	130	88	1	6	133	2	131	2.31e-14	68.2
MS.gene29017.t1	AT3G27940	30.769	130	88	1	6	133	2	131	2.31e-14	68.2
MS.gene29017.t1	AT5G15060	41.096	73	41	1	10	80	18	90	1.29e-12	63.2

Find 24 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGTTTGAGAGATCCTGTTTA+TGG	0.254736	3.2:-66436300	MS.gene29017:CDS
ACCATTAACTGATTCTGAAA+AGG	0.386367	3.2:+66436042	None:intergenic
ACCTACGTTCACAAAGTGTT+TGG	0.430629	3.2:-66436411	MS.gene29017:CDS
CCATTAACTGATTCTGAAAA+GGG	0.443959	3.2:+66436043	None:intergenic
AGACCAACACAACCATAAAC+AGG	0.459225	3.2:+66436288	None:intergenic
AAAGAACTTGCTACTTATGT+TGG	0.467080	3.2:-66436207	MS.gene29017:CDS
TTTGTCAAAGAAGGTGTTCA+AGG	0.477381	3.2:-66435965	MS.gene29017:CDS
GATCCTGTTTATGGTTGTGT+TGG	0.503197	3.2:-66436291	MS.gene29017:CDS
GAAGGTGTTCAAGGCAGAAA+GGG	0.510547	3.2:-66435956	MS.gene29017:CDS
AGAAGGTGTTCAAGGCAGAA+AGG	0.524281	3.2:-66435957	MS.gene29017:CDS
TGAATCTTGCACGCTGCACA+CGG	0.560567	3.2:+66436501	None:intergenic
GAACTCGACGCCGCCGTCAT+CGG	0.569433	3.2:+66436162	None:intergenic
AGAAAGGGACAACCTTGGAG+AGG	0.582482	3.2:-66435941	MS.gene29017:CDS
AAGGCAGAAAGGGACAACCT+TGG	0.600174	3.2:-66435946	MS.gene29017:CDS
GAAAGGGACAACCTTGGAGA+GGG	0.609665	3.2:-66435940	MS.gene29017:intron
GACGCCGCCGTCATCGGTGT+AGG	0.623332	3.2:+66436168	None:intergenic
CATGCTTCCTACACCGATGA+CGG	0.624741	3.2:-66436175	MS.gene29017:CDS
CAGTGATCATACCCTCTCCA+AGG	0.636121	3.2:+66435929	None:intergenic
GGTGTAGGAAGCATGATCTG+AGG	0.695632	3.2:+66436183	None:intergenic
GCTTCCTACACCGATGACGG+CGG	0.697155	3.2:-66436172	MS.gene29017:CDS
CGTGGCAAAGTTACTCAACG+AGG	0.719799	3.2:-66436379	MS.gene29017:CDS
TCAGCGTCGAAAATGCACAA+AGG	0.734134	3.2:-66436481	MS.gene29017:CDS
AGTGTTTGGTGCTAGCAACG+TGG	0.743523	3.2:-66436397	MS.gene29017:CDS
ACCAAACACTTTGTGAACGT+AGG	0.770701	3.2:+66436410	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	CTTTTGTTAATTATTCTATT+AGG	-	chr3.2:66436518-66436537	MS.gene29017:CDS	15.0%
!!	ACAAAAGTAACAAACAAAAA+AGG	+	chr3.2:66436505-66436524	None:intergenic	20.0%
	CAAACAAAAAAGGACAAAAG+TGG	+	chr3.2:66436495-66436514	None:intergenic	30.0%
!	ACCATTAACTGATTCTGAAA+AGG	+	chr3.2:66436337-66436356	None:intergenic	30.0%
!	CCATTAACTGATTCTGAAAA+GGG	+	chr3.2:66436336-66436355	None:intergenic	30.0%
!!	AAAGAACTTGCTACTTATGT+TGG	-	chr3.2:66436169-66436188	MS.gene29017:CDS	30.0%
!!	GTAAACTTTTGAGGATTTTC+CGG	+	chr3.2:66435947-66435966	None:intergenic	30.0%
	ATCATCATCATCATCATCAC+AGG	-	chr3.2:66436360-66436379	MS.gene29017:CDS	35.0%
!	CCCTTTTCAGAATCAGTTAA+TGG	-	chr3.2:66436333-66436352	MS.gene29017:CDS	35.0%
!	TGAACGTAGGTAAACTTTTG+AGG	+	chr3.2:66435956-66435975	None:intergenic	35.0%
!	TTTGACAAAAATGCGTCGAT+GGG	+	chr3.2:66436400-66436419	None:intergenic	35.0%
!!	AAACGAGTTTTCAGCATTGA+AGG	+	chr3.2:66436293-66436312	None:intergenic	35.0%
!!	TTTGTCAAAGAAGGTGTTCA+AGG	-	chr3.2:66436411-66436430	MS.gene29017:CDS	35.0%
	ACCAAACACTTTGTGAACGT+AGG	+	chr3.2:66435969-66435988	None:intergenic	40.0%
	AGACCAACACAACCATAAAC+AGG	+	chr3.2:66436091-66436110	None:intergenic	40.0%
	CGTTTGAGAGATCCTGTTTA+TGG	-	chr3.2:66436076-66436095	MS.gene29017:CDS	40.0%
	CTTTGACAAAAATGCGTCGA+TGG	+	chr3.2:66436401-66436420	None:intergenic	40.0%
	GATCCTGTTTATGGTTGTGT+TGG	-	chr3.2:66436085-66436104	MS.gene29017:CDS	40.0%
!	ACCTACGTTCACAAAGTGTT+TGG	-	chr3.2:66435965-66435984	MS.gene29017:CDS	40.0%
!	CGACGCATTTTTGTCAAAGA+AGG	-	chr3.2:66436402-66436421	MS.gene29017:CDS	40.0%
!!	AACTTTTGAGGATTTTCCGG+CGG	+	chr3.2:66435944-66435963	None:intergenic	40.0%
!	AGGATTTTCCGGCGGAAAAT+AGG	+	chr3.2:66435936-66435955	None:intergenic	45.0%
!	GGATTTTCCGGCGGAAAATA+GGG	+	chr3.2:66435935-66435954	None:intergenic	45.0%
!	TCAGCGTCGAAAATGCACAA+AGG	-	chr3.2:66435895-66435914	MS.gene29017:intron	45.0%
!	TTTTGCACCCTATTTTCCGC+CGG	-	chr3.2:66435925-66435944	MS.gene29017:intron	45.0%
!!	AGAAGGTGTTCAAGGCAGAA+AGG	-	chr3.2:66436419-66436438	MS.gene29017:CDS	45.0%
!!	GAAGGTGTTCAAGGCAGAAA+GGG	-	chr3.2:66436420-66436439	MS.gene29017:CDS	45.0%
	AAGGCAGAAAGGGACAACCT+TGG	-	chr3.2:66436430-66436449	MS.gene29017:CDS	50.0%
	AGAAAGGGACAACCTTGGAG+AGG	-	chr3.2:66436435-66436454	MS.gene29017:CDS	50.0%
	CAGTGATCATACCCTCTCCA+AGG	+	chr3.2:66436450-66436469	None:intergenic	50.0%
	CATGCTTCCTACACCGATGA+CGG	-	chr3.2:66436201-66436220	MS.gene29017:CDS	50.0%
	CGTGGCAAAGTTACTCAACG+AGG	-	chr3.2:66435997-66436016	MS.gene29017:CDS	50.0%
	GAAAGGGACAACCTTGGAGA+GGG	-	chr3.2:66436436-66436455	MS.gene29017:CDS	50.0%
	TGAATCTTGCACGCTGCACA+CGG	+	chr3.2:66435878-66435897	None:intergenic	50.0%
!	GGTGTAGGAAGCATGATCTG+AGG	+	chr3.2:66436196-66436215	None:intergenic	50.0%
!!	AGTGTTTGGTGCTAGCAACG+TGG	-	chr3.2:66435979-66435998	MS.gene29017:CDS	50.0%
!	GCTTCCTACACCGATGACGG+CGG	-	chr3.2:66436204-66436223	MS.gene29017:CDS	60.0%
	GAACTCGACGCCGCCGTCAT+CGG	+	chr3.2:66436217-66436236	None:intergenic	65.0%
	GACGCCGCCGTCATCGGTGT+AGG	+	chr3.2:66436211-66436230	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.2	gene	66435851	66436547	66435851	ID=MS.gene29017
chr3.2	mRNA	66435851	66436547	66435851	ID=MS.gene29017.t1;Parent=MS.gene29017
chr3.2	exon	66435941	66436547	66435941	ID=MS.gene29017.t1.exon1;Parent=MS.gene29017.t1
chr3.2	CDS	66435941	66436547	66435941	ID=cds.MS.gene29017.t1;Parent=MS.gene29017.t1
chr3.2	exon	66435851	66435858	66435851	ID=MS.gene29017.t1.exon2;Parent=MS.gene29017.t1
chr3.2	CDS	66435851	66435858	66435851	ID=cds.MS.gene29017.t1;Parent=MS.gene29017.t1

Gene Sequence

>MS.gene29017

ATGATGTCGTCGTGTAATTCTTCTCCGTGTGCAGCGTGCAAGATTCAGCGTCGAAAATGCACAAAGGAATGCATTTTTGCACCCTATTTTCCGCCGGAAAATCCTCAAAAGTTTACCTACGTTCACAAAGTGTTTGGTGCTAGCAACGTGGCAAAGTTACTCAACGAGGTTGATGCTTCTCAGCGTGAAGATGCTGTTAAGTCTTTAGCTTATGAAGCAGAAGCGCGTTTGAGAGATCCTGTTTATGGTTGTGTTGGTCTTATTTCACATCTTCAGAATAAGCTTAAGAATTTGCAGAATGAACTTAATGTTGCTAAGAAAGAACTTGCTACTTATGTTGGTCCTCAGATCATGCTTCCTACACCGATGACGGCGGCGTCGAGTTCTTATTTTTCTGTGACTACTTCTTCTGTTCATCAACAGCGTCAAGAGTTTTTCACCTTCAATGCTGAAAACTCGTTTTCTAATAACTTTCAGATGAACCCTTTTCAGAATCAGTTAATGGTTTCATCATCATCATCATCATCACAGGCACAGTCATCACAACCCATCGACGCATTTTTGTCAAAGAAGGTGTTCAAGGCAGAAAGGGACAACCTTGGAGAGGGTATGATCACTGTTTCATCTATTTTATTGATTTTCCACTTTTGTCCTTTTTTGTTTGTTACTTTTGTTAATTATTCTATTAGGTTTTTAG

Protein sequence

>MS.gene29017.t1

MMSSCNSSPCAACKIQRRKCTKECIFAPYFPPENPQKFTYVHKVFGASNVAKLLNEVDASQREDAVKSLAYEAEARLRDPVYGCVGLISHLQNKLKNLQNELNVAKKELATYVGPQIMLPTPMTAASSSYFSVTTSSVHQQRQEFFTFNAENSFSNNFQMNPFQNQLMVSSSSSSSQAQSSQPIDAFLSKKVFKAERDNLGEGF