AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene29027

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene29027.t1 MTR_3g073560 97.989 348 7 0 1 348 1 348 0.0 706
MS.gene29027.t1 MTR_5g060950 66.286 350 110 4 1 344 1 348 7.62e-161 454
MS.gene29027.t1 MTR_3g087940 49.425 348 171 2 1 345 14 359 4.72e-117 343
MS.gene29027.t1 MTR_8g091650 45.507 345 183 3 1 345 15 354 2.53e-99 298
MS.gene29027.t1 MTR_7g089800 46.471 340 159 5 13 345 98 421 3.75e-96 292
MS.gene29027.t1 MTR_7g089800 46.471 340 159 5 13 345 127 450 1.39e-95 291
MS.gene29027.t1 MTR_8g040120 50.000 302 140 2 53 347 169 466 1.72e-95 292
MS.gene29027.t1 MTR_8g091650 42.063 378 184 5 1 345 15 390 1.98e-93 284
MS.gene29027.t1 MTR_8g091650 50.719 278 136 1 68 345 5 281 1.56e-92 278
MS.gene29027.t1 MTR_2g086420 49.477 287 138 3 65 351 193 472 1.66e-87 272
MS.gene29027.t1 MTR_2g086420 49.477 287 138 3 65 351 194 473 1.70e-87 272
MS.gene29027.t1 MTR_7g092750 43.824 340 172 6 24 345 22 360 1.39e-83 258
MS.gene29027.t1 MTR_7g092750 43.824 340 172 6 24 345 123 461 1.60e-82 258
MS.gene29027.t1 MTR_4g053250 43.125 320 172 2 32 351 156 465 9.41e-82 257
MS.gene29027.t1 MTR_5g054900 43.793 290 152 2 63 345 66 351 2.78e-80 249
MS.gene29027.t1 MTR_5g054900 43.793 290 152 2 63 345 147 432 3.25e-79 249
MS.gene29027.t1 MTR_1g111310 43.564 303 161 4 50 345 406 705 4.37e-77 251
MS.gene29027.t1 MTR_1g111310 42.034 295 161 2 50 341 39 326 1.14e-68 228
MS.gene29027.t1 MTR_5g054900 43.103 290 150 3 63 345 66 347 1.89e-76 239
MS.gene29027.t1 MTR_5g054900 43.103 290 150 3 63 345 177 458 6.10e-75 239
MS.gene29027.t1 MTR_7g092750 40.294 340 158 7 24 345 123 435 4.98e-68 220
MS.gene29027.t1 MTR_0126s0080 46.988 249 124 2 60 300 211 459 1.83e-65 214
MS.gene29027.t1 MTR_3g044820 42.308 182 96 3 50 223 30 210 7.11e-43 148
MS.gene29027.t1 MTR_1g090370 35.329 167 89 5 167 332 72 220 1.41e-17 81.3
MS.gene29027.t1 MTR_1g111320 55.556 63 28 0 50 112 131 193 2.10e-14 72.0
MS.gene29027.t1 MTR_2g076140 28.571 196 104 5 154 329 23 202 1.41e-13 69.7
MS.gene29027.t1 MTR_7g068860 29.050 179 111 4 171 337 45 219 2.30e-12 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene29027.t1 AT1G22070 50.824 364 158 4 1 345 19 380 1.05e-125 366
MS.gene29027.t1 AT1G77920 49.304 359 158 4 1 345 16 364 6.75e-118 346
MS.gene29027.t1 AT5G10030 48.127 347 174 2 1 343 14 358 1.43e-113 335
MS.gene29027.t1 AT5G10030 48.127 347 174 2 1 343 14 358 1.43e-113 335
MS.gene29027.t1 AT5G10030 48.127 347 174 2 1 343 14 358 1.43e-113 335
MS.gene29027.t1 AT5G10030 48.127 347 174 2 1 343 14 358 1.43e-113 335
MS.gene29027.t1 AT5G10030 48.127 347 174 2 1 343 14 358 1.43e-113 335
MS.gene29027.t1 AT5G65210 48.703 347 172 2 3 343 16 362 5.58e-113 333
MS.gene29027.t1 AT5G65210 48.703 347 172 2 3 343 16 362 5.58e-113 333
MS.gene29027.t1 AT5G65210 48.703 347 172 2 3 343 16 362 5.58e-113 333
MS.gene29027.t1 AT5G65210 48.703 347 172 2 3 343 16 362 5.58e-113 333
MS.gene29027.t1 AT5G65210 48.703 347 172 2 3 343 16 362 5.58e-113 333
MS.gene29027.t1 AT5G65210 48.703 347 172 2 3 343 16 362 5.58e-113 333
MS.gene29027.t1 AT5G65210 52.778 288 136 0 56 343 5 292 7.56e-106 313
MS.gene29027.t1 AT3G12250 47.826 299 146 2 53 345 7 301 7.96e-91 275
MS.gene29027.t1 AT3G12250 47.826 299 146 2 53 345 34 328 1.49e-90 275
MS.gene29027.t1 AT3G12250 47.826 299 146 2 53 345 34 328 1.49e-90 275
MS.gene29027.t1 AT3G12250 47.826 299 146 2 53 345 34 328 1.49e-90 275
MS.gene29027.t1 AT3G12250 47.826 299 146 2 53 345 34 328 1.49e-90 275
MS.gene29027.t1 AT3G12250 46.154 312 158 2 40 345 15 322 1.51e-90 275
MS.gene29027.t1 AT5G06950 47.492 299 147 2 53 345 34 328 1.68e-90 275
MS.gene29027.t1 AT5G06950 47.492 299 147 2 53 345 34 328 1.68e-90 275
MS.gene29027.t1 AT5G06950 47.492 299 147 2 53 345 34 328 1.68e-90 275
MS.gene29027.t1 AT5G06950 47.492 299 147 2 53 345 34 328 1.68e-90 275
MS.gene29027.t1 AT5G06950 47.492 299 147 2 53 345 34 328 1.68e-90 275
MS.gene29027.t1 AT3G12250 47.826 299 146 2 53 345 59 353 7.50e-90 274
MS.gene29027.t1 AT5G06960 49.135 289 137 2 63 345 24 308 1.41e-89 271
MS.gene29027.t1 AT5G06960 49.135 289 137 2 63 345 44 328 2.01e-89 272
MS.gene29027.t1 AT5G06960 49.135 289 137 2 63 345 44 328 2.01e-89 272
MS.gene29027.t1 AT1G08320 43.769 329 165 4 32 351 16 333 4.95e-88 269
MS.gene29027.t1 AT1G08320 43.769 329 165 4 32 351 16 333 4.95e-88 269
MS.gene29027.t1 AT1G08320 43.769 329 165 4 32 351 140 457 6.18e-86 268
MS.gene29027.t1 AT1G08320 43.769 329 165 4 32 351 140 457 6.18e-86 268
MS.gene29027.t1 AT1G08320 43.769 329 165 4 32 351 140 457 6.18e-86 268
MS.gene29027.t1 AT5G06839 45.574 305 143 4 60 345 113 413 3.70e-81 254
MS.gene29027.t1 AT5G06839 45.425 306 143 4 60 345 113 414 4.73e-81 253
MS.gene29027.t1 AT5G06839 45.574 305 143 4 60 345 156 456 9.20e-81 254
MS.gene29027.t1 AT1G68640 42.905 296 157 3 60 347 161 452 4.51e-74 236
MS.gene29027.t1 AT3G14880 23.077 234 141 5 133 341 6 225 3.03e-11 63.2
MS.gene29027.t1 AT1G58330 26.816 179 118 4 171 337 46 223 3.07e-11 63.2

Find 84 sgRNAs with CRISPR-Local

Find 129 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCATGATTTGCTGCATTTAA+TGG 0.080172 3.4:+72149072 None:intergenic
TTGAAATTGAATACGGAAAT+TGG 0.106575 3.4:-72149576 MS.gene29027:CDS
TTCGTACTATTTGTTCTCTA+TGG 0.263825 3.4:-72148679 MS.gene29027:CDS
CTAGGGGCTTGATTGCTCTT+GGG 0.281037 3.4:-72148718 MS.gene29027:CDS
TCCCATAAAACTGGTATCTA+AGG 0.348603 3.4:+72149710 None:intergenic
CACCCCACATGCTAACTTGC+TGG 0.350263 3.4:+72150634 None:intergenic
GCTAGGGGCTTGATTGCTCT+TGG 0.359255 3.4:-72148719 MS.gene29027:CDS
CACCTAGGATTACTCCTTAG+AGG 0.368350 3.4:-72148628 None:intergenic
GAAAAGCCGGGAGCGCAAAA+AGG 0.370740 3.4:-72150180 MS.gene29027:intron
ATGCAATTGGAGCTGGAGAT+TGG 0.373155 3.4:-72149880 MS.gene29027:CDS
TGAAATTGAATACGGAAATT+GGG 0.386776 3.4:-72149575 MS.gene29027:CDS
ACAAGAGACTACAAACAATA+AGG 0.391876 3.4:-72150355 MS.gene29027:intron
TTCAGATGATCCCATAAAAC+TGG 0.394744 3.4:+72149701 None:intergenic
TCCTTGTGAAAGAGCATCTT+CGG 0.402772 3.4:+72149140 None:intergenic
CAGATTGAAGCTCATGCAAT+TGG 0.405773 3.4:-72149893 MS.gene29027:CDS
GTTGCGTTTGAAATTGAATA+CGG 0.408058 3.4:-72149583 MS.gene29027:CDS
AAATAAACTCCAGGAGAGTC+TGG 0.422863 3.4:-72149115 MS.gene29027:CDS
TGAGCTCTTCGTTCCGCCTA+TGG 0.423220 3.4:+72149542 None:intergenic
TGCAATTGGAGCTGGAGATT+GGG 0.428890 3.4:-72149879 MS.gene29027:CDS
TCAGCTTCTGAATGATCCCT+TGG 0.431043 3.4:+72150378 None:intergenic
AGTAATCCTAGGTGATATCT+TGG 0.431819 3.4:+72148637 None:intergenic
GTCCGAAGTGCAGCTTCGTC+TGG 0.433393 3.4:-72149497 MS.gene29027:CDS
ATGGAACAAATGCCATGTCT+TGG 0.464262 3.4:+72149091 None:intergenic
GGTGACAGCTTTAAAGTTGA+AGG 0.469579 3.4:-72150615 MS.gene29027:CDS
CCATTAAATGCAGCAAATCA+TGG 0.469880 3.4:-72149072 MS.gene29027:CDS
GTCGACAGGCAGATCATCTC+AGG 0.470981 3.4:-72148790 MS.gene29027:intron
GTCTACACACCAAGGTGCTA+GGG 0.472353 3.4:-72148735 MS.gene29027:CDS
TAGGGGCTTGATTGCTCTTG+GGG 0.473739 3.4:-72148717 MS.gene29027:CDS
TGTCTTGGACCAGACTCTCC+TGG 0.474340 3.4:+72149106 None:intergenic
CACATGCTAACTTGCTGGAA+TGG 0.476844 3.4:+72150639 None:intergenic
TAGCACCTTGGTGTGTAGAC+AGG 0.499406 3.4:+72148738 None:intergenic
ATCCTCCGATCCATAGGAAA+AGG 0.500037 3.4:+72149362 None:intergenic
CACTACTAGCATGGAGAACA+AGG 0.505528 3.4:-72150547 MS.gene29027:intron
TGTCTACACACCAAGGTGCT+AGG 0.507663 3.4:-72148736 MS.gene29027:CDS
CCATAGGAAAAGGCGCTCTA+CGG 0.507714 3.4:+72149372 None:intergenic
GATTGGGAGAGCAAGAAAGC+AGG 0.518438 3.4:-72149863 MS.gene29027:intron
TATTGCTATAGACAAATTTG+AGG 0.520557 3.4:-72149037 MS.gene29027:CDS
TAGTTGCAGGGTATTTGCAC+AGG 0.526630 3.4:-72149738 MS.gene29027:intron
TGTACATTTAGAAGTTGTGA+TGG 0.532177 3.4:+72149334 None:intergenic
GAAGCTCATGCAATTGGAGC+TGG 0.532336 3.4:-72149887 MS.gene29027:CDS
GAGAGGCTGCTAAGAAAAGC+CGG 0.533392 3.4:-72150193 MS.gene29027:CDS
AATGAAAACGGATGCTGCAA+AGG 0.533573 3.4:-72149434 MS.gene29027:CDS
GATCCCCTCTATTTAATCTC+CGG 0.535169 3.4:-72149409 MS.gene29027:CDS
TTCCTCTAAGGAGTAATCCT+AGG 0.546737 3.4:+72148626 None:intergenic
TTACTGTTCACTAGTAACAT+TGG 0.550321 3.4:+72150570 None:intergenic
ATTGCTATAGACAAATTTGA+GGG 0.550505 3.4:-72149036 MS.gene29027:CDS
CAGCCTCTCGATTCTGTGAG+AGG 0.550635 3.4:+72150207 None:intergenic
GACAAATTTGAGGGACTCGA+AGG 0.552796 3.4:-72149027 MS.gene29027:CDS
GTACATTTAGAAGTTGTGAT+GGG 0.555666 3.4:+72149335 None:intergenic
ACACACCGGAGATTAAATAG+AGG 0.563190 3.4:+72149404 None:intergenic
ATTGGGTTGACGAGAACCAT+AGG 0.567572 3.4:-72149558 MS.gene29027:CDS
GGTGATATCTTGGAATCGAA+TGG 0.569812 3.4:+72148647 None:intergenic
CTTGGTGTGTAGACAGGATG+CGG 0.573164 3.4:+72148744 None:intergenic
TCTATTTAATCTCCGGTGTG+TGG 0.577155 3.4:-72149402 MS.gene29027:CDS
AGGAAAAGGCGCTCTACGGA+TGG 0.579044 3.4:+72149376 None:intergenic
TCAGACAAAGGCTCAAGTTG+TGG 0.581334 3.4:+72149210 None:intergenic
CACACCGGAGATTAAATAGA+GGG 0.585253 3.4:+72149405 None:intergenic
GAATGATCCCTTGGTTCATG+AGG 0.586065 3.4:+72150387 None:intergenic
CATTCCAGCAAGTTAGCATG+TGG 0.586492 3.4:-72150638 MS.gene29027:CDS
AGAGGCTGCTAAGAAAAGCC+GGG 0.588682 3.4:-72150192 MS.gene29027:CDS
ACAAGGACTAAATAAACTCC+AGG 0.589120 3.4:-72149124 MS.gene29027:CDS
GAACAGTAACACTACTAGCA+TGG 0.589169 3.4:-72150556 MS.gene29027:CDS
GGCGAGATCCTCCGATCCAT+AGG 0.593085 3.4:+72149356 None:intergenic
GAGTGTCTTGAATCATTACT+CGG 0.593826 3.4:-72149467 MS.gene29027:CDS
CAATCAAGCCCCTAGCACCT+TGG 0.595789 3.4:+72148726 None:intergenic
TTCCAGCAAGTTAGCATGTG+GGG 0.599412 3.4:-72150636 MS.gene29027:CDS
AACCAGACGAAGCTGCACTT+CGG 0.601003 3.4:+72149495 None:intergenic
GCCGAAGATGCTCTTTCACA+AGG 0.601136 3.4:-72149141 MS.gene29027:CDS
AATATATTCCTCATGAACCA+AGG 0.603855 3.4:-72150395 MS.gene29027:CDS
AACATTTGTTCATCAGACAA+AGG 0.607845 3.4:+72149198 None:intergenic
GGAGAGTCTGGTCCAAGACA+TGG 0.608703 3.4:-72149103 MS.gene29027:CDS
AGCAAATCATGGAATTCAGA+TGG 0.610088 3.4:-72149061 MS.gene29027:CDS
TACGGATGGTTTCCACACAC+CGG 0.623678 3.4:+72149390 None:intergenic
ATATATTCCTCATGAACCAA+GGG 0.628071 3.4:-72150394 MS.gene29027:CDS
GACAGCTTTAAAGTTGAAGG+TGG 0.628685 3.4:-72150612 MS.gene29027:CDS
TTGGTGTGTAGACAGGATGC+GGG 0.633617 3.4:+72148745 None:intergenic
TCTACACACCAAGGTGCTAG+GGG 0.638389 3.4:-72148734 MS.gene29027:CDS
ATTCCAGCAAGTTAGCATGT+GGG 0.642837 3.4:-72150637 MS.gene29027:CDS
TCATCTGAAACAATTAACCC+AGG 0.648000 3.4:-72149687 MS.gene29027:intron
CGCATCCTGTCTACACACCA+AGG 0.659708 3.4:-72148743 MS.gene29027:CDS
TCGATTCCAAGATATCACCT+AGG 0.670756 3.4:-72148643 MS.gene29027:CDS
ACGCCTCTCACAGAATCGAG+AGG 0.691290 3.4:-72150210 MS.gene29027:CDS
ACACCGGAGATTAAATAGAG+GGG 0.728769 3.4:+72149406 None:intergenic
GGGTTGACGAGAACCATAGG+CGG 0.739081 3.4:-72149555 MS.gene29027:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGGTAATATAATTTACTTAT+AGG - chr3.4:72149125-72149144 MS.gene29027:CDS 15.0%
!! TAGCGAATTAGATTAAAAAT+TGG - chr3.4:72149987-72150006 MS.gene29027:intron 20.0%
!! TCAATTTGATAAATTGTGAA+TGG - chr3.4:72150464-72150483 MS.gene29027:intron 20.0%
!!! AAATGTTTTGTGATAAAACA+TGG - chr3.4:72149003-72149022 MS.gene29027:intron 20.0%
!!! GGTAAATAAATTGTTAGTTT+TGG + chr3.4:72149452-72149471 None:intergenic 20.0%
!!! GTTTTATCACAAAACATTTT+TGG + chr3.4:72149002-72149021 None:intergenic 20.0%
! AAAATTTCTACTTTAGTTGC+AGG - chr3.4:72149534-72149553 MS.gene29027:CDS 25.0%
! AAATTTCTACTTTAGTTGCA+GGG - chr3.4:72149535-72149554 MS.gene29027:CDS 25.0%
! AAGCATATGTTTCATATTTG+TGG + chr3.4:72149334-72149353 None:intergenic 25.0%
! ATATAAATCAGAAGTGAAGA+TGG + chr3.4:72149473-72149492 None:intergenic 25.0%
! ATTGCTATAGACAAATTTGA+GGG - chr3.4:72150249-72150268 MS.gene29027:intron 25.0%
! CTAGTTATAATTTGAACATC+AGG + chr3.4:72150362-72150381 None:intergenic 25.0%
! GAACAATGTAATGATGAATA+TGG + chr3.4:72150446-72150465 None:intergenic 25.0%
! TAGTTAGTTAGTTAGTTAGT+TGG + chr3.4:72149283-72149302 None:intergenic 25.0%
! TATTGCTATAGACAAATTTG+AGG - chr3.4:72150248-72150267 MS.gene29027:intron 25.0%
! TGAAATTGAATACGGAAATT+GGG - chr3.4:72149710-72149729 MS.gene29027:CDS 25.0%
! TTGAAATTGAATACGGAAAT+TGG - chr3.4:72149709-72149728 MS.gene29027:CDS 25.0%
AACATTTGTTCATCAGACAA+AGG + chr3.4:72150090-72150109 None:intergenic 30.0%
AATATATTCCTCATGAACCA+AGG - chr3.4:72148890-72148909 MS.gene29027:intron 30.0%
ACAAGAGACTACAAACAATA+AGG - chr3.4:72148930-72148949 MS.gene29027:intron 30.0%
ATATATTCCTCATGAACCAA+GGG - chr3.4:72148891-72148910 MS.gene29027:intron 30.0%
ATATCAAGAATCACAAACCT+GGG + chr3.4:72149618-72149637 None:intergenic 30.0%
CCAAGAAATTTGAATCAACT+AGG - chr3.4:72150305-72150324 MS.gene29027:intron 30.0%
GTACATTTAGAAGTTGTGAT+GGG + chr3.4:72149953-72149972 None:intergenic 30.0%
TAATGATTAGTGATTCGTTG+AGG + chr3.4:72150024-72150043 None:intergenic 30.0%
TGTACATTTAGAAGTTGTGA+TGG + chr3.4:72149954-72149973 None:intergenic 30.0%
TTACTGTTCACTAGTAACAT+TGG + chr3.4:72148718-72148737 None:intergenic 30.0%
TTCGTACTATTTGTTCTCTA+TGG - chr3.4:72150606-72150625 MS.gene29027:CDS 30.0%
! CCTAGTTGATTCAAATTTCT+TGG + chr3.4:72150308-72150327 None:intergenic 30.0%
! GGATCTTTTCAGAATGAAAA+CGG - chr3.4:72149839-72149858 MS.gene29027:intron 30.0%
! GTTGCGTTTGAAATTGAATA+CGG - chr3.4:72149702-72149721 MS.gene29027:CDS 30.0%
! TGTTCCTTTGTTGTGTAAAA+CGG - chr3.4:72149163-72149182 MS.gene29027:CDS 30.0%
!! AACTGTTCTATTGAGTTGAA+TGG + chr3.4:72149218-72149237 None:intergenic 30.0%
!! ACTGTTCTATTGAGTTGAAT+GGG + chr3.4:72149217-72149236 None:intergenic 30.0%
ACAAGGACTAAATAAACTCC+AGG - chr3.4:72150161-72150180 MS.gene29027:intron 35.0%
ACATATGCTTGATATTGTGC+AGG - chr3.4:72149344-72149363 MS.gene29027:CDS 35.0%
ACTGTCATCCCAAAATAATG+AGG + chr3.4:72150391-72150410 None:intergenic 35.0%
AGAGTCTGACTCATTTAATG+TGG - chr3.4:72148821-72148840 MS.gene29027:intron 35.0%
AGCAAATCATGGAATTCAGA+TGG - chr3.4:72150224-72150243 MS.gene29027:intron 35.0%
AGTAATCCTAGGTGATATCT+TGG + chr3.4:72150651-72150670 None:intergenic 35.0%
ATCTTTGTGTTCTGTTAACC+AGG - chr3.4:72149675-72149694 MS.gene29027:intron 35.0%
CATCCCAAAATAATGAGGAA+GGG + chr3.4:72150386-72150405 None:intergenic 35.0%
CCATTAAATGCAGCAAATCA+TGG - chr3.4:72150213-72150232 MS.gene29027:CDS 35.0%
GAGTGTCTTGAATCATTACT+CGG - chr3.4:72149818-72149837 MS.gene29027:intron 35.0%
GATATCAAGAATCACAAACC+TGG + chr3.4:72149619-72149638 None:intergenic 35.0%
GGTTGAGGAAATGAAAATTG+AGG - chr3.4:72149184-72149203 MS.gene29027:CDS 35.0%
TCATCCCAAAATAATGAGGA+AGG + chr3.4:72150387-72150406 None:intergenic 35.0%
TCCCATAAAACTGGTATCTA+AGG + chr3.4:72149578-72149597 None:intergenic 35.0%
TCTTTGTGTTCTGTTAACCA+GGG - chr3.4:72149676-72149695 MS.gene29027:intron 35.0%
TTCAGATGATCCCATAAAAC+TGG + chr3.4:72149587-72149606 None:intergenic 35.0%
! AGACCCTTCCTCATTATTTT+GGG - chr3.4:72150380-72150399 MS.gene29027:CDS 35.0%
! TAGACCCTTCCTCATTATTT+TGG - chr3.4:72150379-72150398 MS.gene29027:CDS 35.0%
! TCAACCGTTTTACACAACAA+AGG + chr3.4:72149170-72149189 None:intergenic 35.0%
! TCATCTGAAACAATTAACCC+AGG - chr3.4:72149598-72149617 MS.gene29027:intron 35.0%
! TTTGTTGTGTAAAACGGTTG+AGG - chr3.4:72149169-72149188 MS.gene29027:CDS 35.0%
!! CCATGATTTGCTGCATTTAA+TGG + chr3.4:72150216-72150235 None:intergenic 35.0%
!! GCCTTAGATACCAGTTTTAT+GGG - chr3.4:72149574-72149593 MS.gene29027:CDS 35.0%
!! TTTCTTGGTGTTACATTACC+TGG + chr3.4:72150293-72150312 None:intergenic 35.0%
AAATAAACTCCAGGAGAGTC+TGG - chr3.4:72150170-72150189 MS.gene29027:intron 40.0%
AATGAAAACGGATGCTGCAA+AGG - chr3.4:72149851-72149870 MS.gene29027:intron 40.0%
ACACACCGGAGATTAAATAG+AGG + chr3.4:72149884-72149903 None:intergenic 40.0%
ACACCGGAGATTAAATAGAG+GGG + chr3.4:72149882-72149901 None:intergenic 40.0%
ATGGAACAAATGCCATGTCT+TGG + chr3.4:72150197-72150216 None:intergenic 40.0%
ATTCCAGCAAGTTAGCATGT+GGG - chr3.4:72148648-72148667 MS.gene29027:CDS 40.0%
ATTTCAAACGCAACAATCCC+TGG + chr3.4:72149696-72149715 None:intergenic 40.0%
CACACCGGAGATTAAATAGA+GGG + chr3.4:72149883-72149902 None:intergenic 40.0%
CAGATTGAAGCTCATGCAAT+TGG - chr3.4:72149392-72149411 MS.gene29027:CDS 40.0%
GAACAGTAACACTACTAGCA+TGG - chr3.4:72148729-72148748 MS.gene29027:CDS 40.0%
GATCCCCTCTATTTAATCTC+CGG - chr3.4:72149876-72149895 MS.gene29027:CDS 40.0%
GGTGATATCTTGGAATCGAA+TGG + chr3.4:72150641-72150660 None:intergenic 40.0%
GTGTCTGTGACATTATGTGT+AGG - chr3.4:72149045-72149064 MS.gene29027:CDS 40.0%
TCGATTCCAAGATATCACCT+AGG - chr3.4:72150642-72150661 MS.gene29027:CDS 40.0%
TCTATTTAATCTCCGGTGTG+TGG - chr3.4:72149883-72149902 MS.gene29027:CDS 40.0%
! ATATTACCTTTTTGCGCTCC+CGG + chr3.4:72149114-72149133 None:intergenic 40.0%
! CGCCTTAGATACCAGTTTTA+TGG - chr3.4:72149573-72149592 MS.gene29027:CDS 40.0%
!! ACTCGAAGGTTTTGTAATCC+AGG - chr3.4:72150272-72150291 MS.gene29027:intron 40.0%
!! GACAGCTTTAAAGTTGAAGG+TGG - chr3.4:72148673-72148692 MS.gene29027:CDS 40.0%
!! GGTGACAGCTTTAAAGTTGA+AGG - chr3.4:72148670-72148689 MS.gene29027:CDS 40.0%
!! TCCTTGTGAAAGAGCATCTT+CGG + chr3.4:72150148-72150167 None:intergenic 40.0%
ATCCTCCGATCCATAGGAAA+AGG + chr3.4:72149926-72149945 None:intergenic 45.0%
ATGCAATTGGAGCTGGAGAT+TGG - chr3.4:72149405-72149424 MS.gene29027:CDS 45.0%
CACTACTAGCATGGAGAACA+AGG - chr3.4:72148738-72148757 MS.gene29027:CDS 45.0%
CATTCCAGCAAGTTAGCATG+TGG - chr3.4:72148647-72148666 MS.gene29027:CDS 45.0%
GAATGATCCCTTGGTTCATG+AGG + chr3.4:72148901-72148920 None:intergenic 45.0%
GACAAATTTGAGGGACTCGA+AGG - chr3.4:72150258-72150277 MS.gene29027:intron 45.0%
TCAGCTTCTGAATGATCCCT+TGG + chr3.4:72148910-72148929 None:intergenic 45.0%
TGCAATTGGAGCTGGAGATT+GGG - chr3.4:72149406-72149425 MS.gene29027:CDS 45.0%
TTCCAGCAAGTTAGCATGTG+GGG - chr3.4:72148649-72148668 MS.gene29027:CDS 45.0%
! GAATGGTTTGTGTTGTCGAC+AGG - chr3.4:72150481-72150500 MS.gene29027:intron 45.0%
! TAGTTGCAGGGTATTTGCAC+AGG - chr3.4:72149547-72149566 MS.gene29027:CDS 45.0%
! TCAGACAAAGGCTCAAGTTG+TGG + chr3.4:72150078-72150097 None:intergenic 45.0%
!! ATTGGGTTGACGAGAACCAT+AGG - chr3.4:72149727-72149746 MS.gene29027:CDS 45.0%
!! CACATGCTAACTTGCTGGAA+TGG + chr3.4:72148649-72148668 None:intergenic 45.0%
AACCAGACGAAGCTGCACTT+CGG + chr3.4:72149793-72149812 None:intergenic 50.0%
AGAGGCTGCTAAGAAAAGCC+GGG - chr3.4:72149093-72149112 MS.gene29027:CDS 50.0%
CTAGGGGCTTGATTGCTCTT+GGG - chr3.4:72150567-72150586 MS.gene29027:CDS 50.0%
GAAGCTCATGCAATTGGAGC+TGG - chr3.4:72149398-72149417 MS.gene29027:CDS 50.0%
GAGAGGCTGCTAAGAAAAGC+CGG - chr3.4:72149092-72149111 MS.gene29027:CDS 50.0%
GATTGGGAGAGCAAGAAAGC+AGG - chr3.4:72149422-72149441 MS.gene29027:CDS 50.0%
GCCGAAGATGCTCTTTCACA+AGG - chr3.4:72150144-72150163 MS.gene29027:intron 50.0%
GTTGTGGCACAATGAGCTGT+AGG + chr3.4:72150062-72150081 None:intergenic 50.0%
TACGGATGGTTTCCACACAC+CGG + chr3.4:72149898-72149917 None:intergenic 50.0%
TAGGGGCTTGATTGCTCTTG+GGG - chr3.4:72150568-72150587 MS.gene29027:CDS 50.0%
TTGGTGTGTAGACAGGATGC+GGG + chr3.4:72150543-72150562 None:intergenic 50.0%
! CTTGGTGTGTAGACAGGATG+CGG + chr3.4:72150544-72150563 None:intergenic 50.0%
! GAGCGCCTTTTCCTATGGAT+CGG - chr3.4:72149918-72149937 MS.gene29027:CDS 50.0%
! GTCTACACACCAAGGTGCTA+GGG - chr3.4:72150550-72150569 MS.gene29027:CDS 50.0%
! TAGCACCTTGGTGTGTAGAC+AGG + chr3.4:72150550-72150569 None:intergenic 50.0%
! TCTACACACCAAGGTGCTAG+GGG - chr3.4:72150551-72150570 MS.gene29027:CDS 50.0%
! TGTCTACACACCAAGGTGCT+AGG - chr3.4:72150549-72150568 MS.gene29027:CDS 50.0%
!! CCATAGGAAAAGGCGCTCTA+CGG + chr3.4:72149916-72149935 None:intergenic 50.0%
ACGCCTCTCACAGAATCGAG+AGG - chr3.4:72149075-72149094 MS.gene29027:CDS 55.0%
CAATCAAGCCCCTAGCACCT+TGG + chr3.4:72150562-72150581 None:intergenic 55.0%
CAGCCTCTCGATTCTGTGAG+AGG + chr3.4:72149081-72149100 None:intergenic 55.0%
CGCATCCTGTCTACACACCA+AGG - chr3.4:72150542-72150561 MS.gene29027:intron 55.0%
GCTAGGGGCTTGATTGCTCT+TGG - chr3.4:72150566-72150585 MS.gene29027:CDS 55.0%
GGAGAGTCTGGTCCAAGACA+TGG - chr3.4:72150182-72150201 MS.gene29027:CDS 55.0%
GGGTTGACGAGAACCATAGG+CGG - chr3.4:72149730-72149749 MS.gene29027:CDS 55.0%
GTCGACAGGCAGATCATCTC+AGG - chr3.4:72150495-72150514 MS.gene29027:intron 55.0%
GTGGCACAATGAGCTGTAGG+AGG + chr3.4:72150059-72150078 None:intergenic 55.0%
TGAGCTCTTCGTTCCGCCTA+TGG + chr3.4:72149746-72149765 None:intergenic 55.0%
TGTCTTGGACCAGACTCTCC+TGG + chr3.4:72150182-72150201 None:intergenic 55.0%
! CACCCCACATGCTAACTTGC+TGG + chr3.4:72148654-72148673 None:intergenic 55.0%
! CGCCTTTTCCTATGGATCGG+AGG - chr3.4:72149921-72149940 MS.gene29027:CDS 55.0%
! GAAAAGCCGGGAGCGCAAAA+AGG - chr3.4:72149105-72149124 MS.gene29027:CDS 55.0%
!! AGGAAAAGGCGCTCTACGGA+TGG + chr3.4:72149912-72149931 None:intergenic 55.0%
!! CCGTAGAGCGCCTTTTCCTA+TGG - chr3.4:72149913-72149932 MS.gene29027:CDS 55.0%
GGCGAGATCCTCCGATCCAT+AGG + chr3.4:72149932-72149951 None:intergenic 60.0%
GTCCGAAGTGCAGCTTCGTC+TGG - chr3.4:72149788-72149807 MS.gene29027:intron 60.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 72148631 72150676 72148631 ID=MS.gene29027
chr3.4 mRNA 72148631 72150676 72148631 ID=MS.gene29027.t1;Parent=MS.gene29027
chr3.4 exon 72150548 72150676 72150548 ID=MS.gene29027.t1.exon1;Parent=MS.gene29027.t1
chr3.4 CDS 72150548 72150676 72150548 ID=cds.MS.gene29027.t1;Parent=MS.gene29027.t1
chr3.4 exon 72150356 72150421 72150356 ID=MS.gene29027.t1.exon2;Parent=MS.gene29027.t1
chr3.4 CDS 72150356 72150421 72150356 ID=cds.MS.gene29027.t1;Parent=MS.gene29027.t1
chr3.4 exon 72150181 72150240 72150181 ID=MS.gene29027.t1.exon3;Parent=MS.gene29027.t1
chr3.4 CDS 72150181 72150240 72150181 ID=cds.MS.gene29027.t1;Parent=MS.gene29027.t1
chr3.4 exon 72149864 72149941 72149864 ID=MS.gene29027.t1.exon4;Parent=MS.gene29027.t1
chr3.4 CDS 72149864 72149941 72149864 ID=cds.MS.gene29027.t1;Parent=MS.gene29027.t1
chr3.4 exon 72149688 72149751 72149688 ID=MS.gene29027.t1.exon5;Parent=MS.gene29027.t1
chr3.4 CDS 72149688 72149751 72149688 ID=cds.MS.gene29027.t1;Parent=MS.gene29027.t1
chr3.4 exon 72149339 72149610 72149339 ID=MS.gene29027.t1.exon6;Parent=MS.gene29027.t1
chr3.4 CDS 72149339 72149610 72149339 ID=cds.MS.gene29027.t1;Parent=MS.gene29027.t1
chr3.4 exon 72149014 72149241 72149014 ID=MS.gene29027.t1.exon7;Parent=MS.gene29027.t1
chr3.4 CDS 72149014 72149241 72149014 ID=cds.MS.gene29027.t1;Parent=MS.gene29027.t1
chr3.4 exon 72148631 72148804 72148631 ID=MS.gene29027.t1.exon8;Parent=MS.gene29027.t1
chr3.4 CDS 72148631 72148804 72148631 ID=cds.MS.gene29027.t1;Parent=MS.gene29027.t1
Gene Sequence

>MS.gene29027

ATGGATATATACGAGCCATTCCAGCAAGTTAGCATGTGGGGTGACAGCTTTAAAGTTGAAGGTGGTCTTAATTCAATTGCTTCTCCAATGTTACTAGTGAACAGTAACACTACTAGCATGGAGAACAAGGTGAGTTATTATAACTAATAATTTGATTTTATTTATTTATTATTGTTACTTTTATGATTATAGAGTCTGACTCATTTAATGTGGAAACTAGATTATATAACAATCTTATGAATTTGAATTTTGCAGTCTGAATATATTCCTCATGAACCAAGGGATCATTCAGAAGCTGAACAAGAGACTACAAACAATAAGGTAATGAAATTTGATCAAGACATAAATTGTGACATTTTGATTTTTGTCCAAAAATGTTTTGTGATAAAACATGGAAGTTTTTGAGTTGTTTATGTGTCTGTGACATTATGTGTAGGTACTTAGACGCCTCTCACAGAATCGAGAGGCTGCTAAGAAAAGCCGGGAGCGCAAAAAGGTAATATAATTTACTTATAGGAAAAGTCATTTGATTTGTTCCTTTGTTGTGTAAAACGGTTGAGGAAATGAAAATTGAGGTTTTTATCCCATTCAACTCAATAGAACAGTTTATAAAGATGTAAGCAGTTATAACTGACTAACAAACATATTTCCAACTAACTAACTAACTAACTAACTTAGTTCTAACTAGCTTTTAACTAATCCACAAATATGAAACATATGCTTGATATTGTGCAGGCTTATGTTAAACAATTAGAATCAAGCAGATTGAAGCTCATGCAATTGGAGCTGGAGATTGGGAGAGCAAGAAAGCAGGTGAACCAAAACTAACAATTTATTTACCATCTTCACTTCTGATTTATATTTATGTTATGCTCTTTCAATTGTAAATTTTAAGCATATATTAAAATTTCTACTTTAGTTGCAGGGTATTTGCACAGGAAGCGCCTTAGATACCAGTTTTATGGGATCATCTGAAACAATTAACCCAGGTTTGTGATTCTTGATATCAATTGTAAGCATTCTTAATATCTTTTATTCTTATTGATCTTTGTGTTCTGTTAACCAGGGATTGTTGCGTTTGAAATTGAATACGGAAATTGGGTTGACGAGAACCATAGGCGGAACGAAGAGCTCAGAAATGCATTTCAAACTAATGCGTCCGAAGTGCAGCTTCGTCTGGTTGTTCAGAGTGTCTTGAATCATTACTCGGATCTTTTCAGAATGAAAACGGATGCTGCAAAGGCTGATCCCCTCTATTTAATCTCCGGTGTGTGGAAACCATCCGTAGAGCGCCTTTTCCTATGGATCGGAGGATCTCGCCCATCACAACTTCTAAATGTACAAATCTGATCTTAATAGCGAATTAGATTAAAAATTGGTACTGTCGAATCCTCAACGAATCACTAATCATTATTAGATTTTTTTCCTCCTACAGCTCATTGTGCCACAACTTGAGCCTTTGTCTGATGAACAAATGTTGAGCATTAAAAAACTTCATCTGTCGTCTCAGCAAGCCGAAGATGCTCTTTCACAAGGACTAAATAAACTCCAGGAGAGTCTGGTCCAAGACATGGCATTTGTTCCATTAAATGCAGCAAATCATGGAATTCAGATGGCTATTGCTATAGACAAATTTGAGGGACTCGAAGGTTTTGTAATCCAGGTAATGTAACACCAAGAAATTTGAATCAACTAGGAATTTTCTAATGCAACTCTTTTATTCTAAGTCCTGATGTTCAAATTATAACTAGACCCTTCCTCATTATTTTGGGATGACAGTTCATATATTATGAATTAGTATTTCTTTATGCTCCATATTCATCATTACATTGTTCAATTTGATAAATTGTGAATGGTTTGTGTTGTCGACAGGCAGATCATCTCAGGCAGCAAACTCTGATACACATGTCCCGCATCCTGTCTACACACCAAGGTGCTAGGGGCTTGATTGCTCTTGGGGAATACTTTCATCGTCTTCGTACTATTTGTTCTCTATGGACTTCTTGTCCATTCGATTCCAAGATATCACCTAGGATTACTCCTTAG
Protein sequence

>MS.gene29027.t1

MDIYEPFQQVSMWGDSFKVEGGLNSIASPMLLVNSNTTSMENKSEYIPHEPRDHSEAEQETTNNKVLRRLSQNREAAKKSRERKKAYVKQLESSRLKLMQLELEIGRARKQGICTGSALDTSFMGSSETINPGIVAFEIEYGNWVDENHRRNEELRNAFQTNASEVQLRLVVQSVLNHYSDLFRMKTDAAKADPLYLISGVWKPSVERLFLWIGGSRPSQLLNLIVPQLEPLSDEQMLSIKKLHLSSQQAEDALSQGLNKLQESLVQDMAFVPLNAANHGIQMAIAIDKFEGLEGFVIQADHLRQQTLIHMSRILSTHQGARGLIALGEYFHRLRTICSLWTSCPFDSKISPRITP