AlfalfaGEDB | MS.gene29045

M. sativa cultivar XinJiangDaYe / MS.gene29045

KEGG: -
GO: GO:0000166 GO:0003674 GO:0003824 GO:0004672 GO:0005488 GO:0005524 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008144 GO:0008150 GO:0008152 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017076 GO:0019538 GO:0030554 GO:0032553 GO:0032555 GO:0032559 GO:0035639 GO:0036094 GO:0036211 GO:0043167 GO:0043168 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0071704 GO:0097159 GO:0097367 GO:0140096 GO:1901265 GO:1901363 GO:1901564
PFAM: PF00069.28

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene29045.t1	PNY06273.1	93	43	3	0	1	43	70	112	2.60E-15	90.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene29045.t1	Q39008	69.6	46	13	1	1	45	496	541	7.5e-11	67.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene29045.t1	A0A2K3NTC4	93.0	43	3	0	1	43	70	112	1.9e-15	90.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene29045.t1	MTR_3g073250	82.222	45	8	0	1	45	446	490	3.04e-21	84.7
MS.gene29045.t1	MTR_3g073250	82.222	45	8	0	1	45	433	477	3.09e-21	84.7
MS.gene29045.t1	MTR_0685s0020	77.778	45	10	0	1	45	154	198	7.65e-21	81.6
MS.gene29045.t1	MTR_5g059940	75.556	45	11	0	1	45	288	332	7.83e-21	83.2
MS.gene29045.t1	MTR_5g059910	80.000	45	9	0	1	45	294	338	3.90e-20	81.3
MS.gene29045.t1	MTR_5g060350	73.333	45	12	0	1	45	484	528	5.24e-20	81.3
MS.gene29045.t1	MTR_5g059880	75.556	45	11	0	1	45	355	399	3.27e-19	79.0
MS.gene29045.t1	MTR_4g094415	74.419	43	11	0	1	43	327	369	6.61e-17	72.4
MS.gene29045.t1	MTR_4g094415	68.889	45	14	0	1	45	327	371	1.85e-15	68.2
MS.gene29045.t1	MTR_4g068740	65.789	38	11	1	1	36	749	786	1.57e-11	57.0
MS.gene29045.t1	MTR_4g068740	65.789	38	11	1	1	36	990	1027	2.61e-11	56.6
MS.gene29045.t1	MTR_3g096230	57.778	45	18	1	1	45	410	453	4.93e-11	55.8
MS.gene29045.t1	MTR_3g096230	57.778	45	18	1	1	45	410	453	5.73e-11	55.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene29045.t1	AT4G08500	69.565	46	13	1	1	45	496	541	1.37e-15	68.6
MS.gene29045.t1	AT4G08480	69.565	46	13	1	1	45	664	709	5.70e-15	67.0
MS.gene29045.t1	AT4G12020	65.957	47	14	1	1	45	1710	1756	1.14e-14	66.2
MS.gene29045.t1	AT4G12020	65.957	47	14	1	1	45	1777	1823	1.25e-14	65.9
MS.gene29045.t1	AT4G12020	65.957	47	14	1	1	45	1785	1831	1.25e-14	65.9
MS.gene29045.t1	AT4G08470	64.444	45	15	1	1	44	466	510	3.24e-13	62.0
MS.gene29045.t1	AT4G08470	64.444	45	15	1	1	44	384	428	3.56e-13	62.0
MS.gene29045.t1	AT4G12020	74.286	35	9	0	11	45	1773	1807	6.29e-13	61.2
MS.gene29045.t1	AT4G12020	74.286	35	9	0	11	45	1781	1815	6.29e-13	61.2

Find 17 sgRNAs with CRISPR-Local

Find 24 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCAAATTGGAGTATGGAATT+TGG	0.170486	3.2:+66054532	None:intergenic
CCAAATTCCATACTCCAATT+TGG	0.210816	3.2:-66054532	MS.gene29045:CDS
CAAGCTGATGTTTGGAGTTT+GGG	0.235597	3.2:-66054582	MS.gene29045:CDS
TCAAGCTGATGTTTGGAGTT+TGG	0.249389	3.2:-66054583	MS.gene29045:CDS
GGCACATACACATTCCAAAT+TGG	0.357007	3.2:+66054518	None:intergenic
ATGGCCTTCAAGCTGATGTT+TGG	0.373571	3.2:-66054590	MS.gene29045:CDS
ACACATTCCAAATTGGAGTA+TGG	0.390116	3.2:+66054525	None:intergenic
AACTCCAAACATCAGCTTGA+AGG	0.428588	3.2:+66054586	None:intergenic
GAGTTTGGGATGCACTGTGC+TGG	0.447799	3.2:-66054568	MS.gene29045:CDS
AAAGGAAAGAACAATGGTTA+TGG	0.453131	3.2:-66054609	MS.gene29045:CDS
TGACACCTACAGAAGGTAAT+GGG	0.465640	3.2:+66054475	None:intergenic
ACTGTGCTGGAGATGCTAAC+TGG	0.470469	3.2:-66054555	MS.gene29045:CDS
CTGACACCTACAGAAGGTAA+TGG	0.492606	3.2:+66054474	None:intergenic
TGGGATATGCAGGTCGTGAA+AGG	0.550867	3.2:-66054627	MS.gene29045:intron
AACATTCTGGATGGCCCCTG+AGG	0.614718	3.2:-66054775	None:intergenic
GTCGTGAAAGGAAAGAACAA+TGG	0.673328	3.2:-66054615	MS.gene29045:CDS
AGATTGAAAGCAAACCTCAG+GGG	0.742082	3.2:+66054761	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATGATTATTAATTATTAGTC+TGG	-	chr3.2:66054603-66054622	MS.gene29045:CDS	15.0%
!!	GACTAATAATTAATAATCAT+TGG	+	chr3.2:66054603-66054622	None:intergenic	15.0%
!!!	TGATTATTAATTATTAGTCT+GGG	-	chr3.2:66054604-66054623	MS.gene29045:CDS	15.0%
	AAAGGAAAGAACAATGGTTA+TGG	-	chr3.2:66054641-66054660	MS.gene29045:intron	30.0%
	GAGAAAAAATAGCAATGACA+TGG	+	chr3.2:66054756-66054775	None:intergenic	30.0%
	TATATCTTGTGTTCATGTTG+AGG	+	chr3.2:66054533-66054552	None:intergenic	30.0%
	AAGATTGAAAGCAAACCTCA+GGG	+	chr3.2:66054493-66054512	None:intergenic	35.0%
	ACACAAGATATAGCAAAGCT+AGG	-	chr3.2:66054541-66054560	MS.gene29045:CDS	35.0%
	ACACATTCCAAATTGGAGTA+TGG	+	chr3.2:66054728-66054747	None:intergenic	35.0%
	CACAAGATATAGCAAAGCTA+GGG	-	chr3.2:66054542-66054561	MS.gene29045:CDS	35.0%
	CCAAATTCCATACTCCAATT+TGG	-	chr3.2:66054718-66054737	MS.gene29045:intron	35.0%
	CCAAATTGGAGTATGGAATT+TGG	+	chr3.2:66054721-66054740	None:intergenic	35.0%
	TAAGATTGAAAGCAAACCTC+AGG	+	chr3.2:66054494-66054513	None:intergenic	35.0%
!	ATTATTAGTCTGGGATATGC+AGG	-	chr3.2:66054613-66054632	MS.gene29045:CDS	35.0%
	AACTCCAAACATCAGCTTGA+AGG	+	chr3.2:66054667-66054686	None:intergenic	40.0%
	AGATTGAAAGCAAACCTCAG+GGG	+	chr3.2:66054492-66054511	None:intergenic	40.0%
	GGCACATACACATTCCAAAT+TGG	+	chr3.2:66054735-66054754	None:intergenic	40.0%
	GTCGTGAAAGGAAAGAACAA+TGG	-	chr3.2:66054635-66054654	MS.gene29045:intron	40.0%
!	CAAGCTGATGTTTGGAGTTT+GGG	-	chr3.2:66054668-66054687	MS.gene29045:intron	40.0%
!	TCAAGCTGATGTTTGGAGTT+TGG	-	chr3.2:66054667-66054686	MS.gene29045:intron	40.0%
	ATGGCCTTCAAGCTGATGTT+TGG	-	chr3.2:66054660-66054679	MS.gene29045:intron	45.0%
	ACTGTGCTGGAGATGCTAAC+TGG	-	chr3.2:66054695-66054714	MS.gene29045:intron	50.0%
	TGGGATATGCAGGTCGTGAA+AGG	-	chr3.2:66054623-66054642	MS.gene29045:intron	50.0%
!	GAGTTTGGGATGCACTGTGC+TGG	-	chr3.2:66054682-66054701	MS.gene29045:intron	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.2	gene	66054485	66054787	66054485	ID=MS.gene29045
chr3.2	mRNA	66054485	66054787	66054485	ID=MS.gene29045.t1;Parent=MS.gene29045
chr3.2	exon	66054776	66054787	66054776	ID=MS.gene29045.t1.exon1;Parent=MS.gene29045.t1
chr3.2	CDS	66054776	66054787	66054776	ID=cds.MS.gene29045.t1;Parent=MS.gene29045.t1
chr3.2	exon	66054485	66054637	66054485	ID=MS.gene29045.t1.exon2;Parent=MS.gene29045.t1
chr3.2	CDS	66054485	66054637	66054485	ID=cds.MS.gene29045.t1;Parent=MS.gene29045.t1

Gene Sequence	>MS.gene29045 ATGGCCCCTGAGGTTTGCTTTCAATCTTAACTTTTCAGTATTATACCTCAACATGAACACAAGATATAGCAAAGCTAGGGATTTTGATATTGCGTATGAAGAATGAGCTTCTATTCCAATGATTATTAATTATTAGTCTGGGATATGCAGGTCGTGAAAGGAAAGAACAATGGTTATGGCCTTCAAGCTGATGTTTGGAGTTTGGGATGCACTGTGCTGGAGATGCTAACTGGCCAAATTCCATACTCCAATTTGGAATGTGTATGTGCCATGTCATTGCTATTTTTTCTCCCATTACCTTCT
Protein sequence	>MS.gene29045.t1 MAPEVVKGKNNGYGLQADVWSLGCTVLEMLTGQIPYSNLECVCAMSLLFFLPLPS