Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29111.t1 | PNY06273.1 | 93 | 43 | 3 | 0 | 1 | 43 | 70 | 112 | 4.70E-15 | 90.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29111.t1 | Q39008 | 69.6 | 46 | 13 | 1 | 1 | 45 | 496 | 541 | 1.8e-10 | 66.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29111.t1 | A0A2K3NTC4 | 93.0 | 43 | 3 | 0 | 1 | 43 | 70 | 112 | 3.4e-15 | 90.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29111.t1 | MTR_3g073250 | 68.333 | 60 | 19 | 0 | 1 | 60 | 446 | 505 | 1.39e-20 | 85.1 |
| MS.gene29111.t1 | MTR_3g073250 | 82.222 | 45 | 8 | 0 | 1 | 45 | 433 | 477 | 1.83e-20 | 84.7 |
| MS.gene29111.t1 | MTR_5g059940 | 75.556 | 45 | 11 | 0 | 1 | 45 | 288 | 332 | 4.57e-20 | 83.2 |
| MS.gene29111.t1 | MTR_0685s0020 | 77.778 | 45 | 10 | 0 | 1 | 45 | 154 | 198 | 1.28e-19 | 80.5 |
| MS.gene29111.t1 | MTR_5g059910 | 80.000 | 45 | 9 | 0 | 1 | 45 | 294 | 338 | 2.27e-19 | 81.3 |
| MS.gene29111.t1 | MTR_5g060350 | 73.333 | 45 | 12 | 0 | 1 | 45 | 484 | 528 | 3.12e-19 | 81.3 |
| MS.gene29111.t1 | MTR_5g059880 | 75.556 | 45 | 11 | 0 | 1 | 45 | 355 | 399 | 2.66e-18 | 78.6 |
| MS.gene29111.t1 | MTR_4g094415 | 74.419 | 43 | 11 | 0 | 1 | 43 | 327 | 369 | 3.77e-16 | 72.4 |
| MS.gene29111.t1 | MTR_4g094415 | 55.556 | 63 | 19 | 2 | 1 | 57 | 327 | 386 | 7.72e-15 | 68.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29111.t1 | AT4G08500 | 69.565 | 46 | 13 | 1 | 1 | 45 | 496 | 541 | 7.98e-15 | 68.6 |
| MS.gene29111.t1 | AT4G08480 | 69.565 | 46 | 13 | 1 | 1 | 45 | 664 | 709 | 3.36e-14 | 67.0 |
| MS.gene29111.t1 | AT4G12020 | 65.957 | 47 | 14 | 1 | 1 | 45 | 1710 | 1756 | 1.19e-13 | 65.5 |
| MS.gene29111.t1 | AT4G12020 | 65.957 | 47 | 14 | 1 | 1 | 45 | 1777 | 1823 | 2.80e-13 | 64.3 |
| MS.gene29111.t1 | AT4G12020 | 65.957 | 47 | 14 | 1 | 1 | 45 | 1785 | 1831 | 2.83e-13 | 64.3 |
| MS.gene29111.t1 | AT4G08470 | 64.444 | 45 | 15 | 1 | 1 | 44 | 466 | 510 | 1.85e-12 | 62.0 |
| MS.gene29111.t1 | AT4G08470 | 64.444 | 45 | 15 | 1 | 1 | 44 | 384 | 428 | 2.00e-12 | 62.0 |
| MS.gene29111.t1 | AT4G12020 | 74.286 | 35 | 9 | 0 | 11 | 45 | 1773 | 1807 | 6.13e-12 | 60.5 |
| MS.gene29111.t1 | AT4G12020 | 74.286 | 35 | 9 | 0 | 11 | 45 | 1781 | 1815 | 6.31e-12 | 60.5 |
Find 22 sgRNAs with CRISPR-Local
Find 61 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCAAATTGGAGTATGGAATT+TGG | 0.170486 | 3.2:+66041566 | None:intergenic |
| CCAAATTCCATACTCCAATT+TGG | 0.210816 | 3.2:-66041566 | MS.gene29111:CDS |
| CAAGCTGATGTTTGGAGTTT+GGG | 0.235597 | 3.2:-66041616 | MS.gene29111:CDS |
| TCAAGCTGATGTTTGGAGTT+TGG | 0.249389 | 3.2:-66041617 | MS.gene29111:CDS |
| ACGTTGCCAAGTTGATAAAT+TGG | 0.329819 | 3.2:+66040881 | None:intergenic |
| GGCACATACACATTCCAAAT+TGG | 0.357007 | 3.2:+66041552 | None:intergenic |
| ATGGCCTTCAAGCTGATGTT+TGG | 0.373571 | 3.2:-66041624 | MS.gene29111:CDS |
| ACACATTCCAAATTGGAGTA+TGG | 0.390116 | 3.2:+66041559 | None:intergenic |
| AACTCCAAACATCAGCTTGA+AGG | 0.428588 | 3.2:+66041620 | None:intergenic |
| GAGTTTGGGATGCACTGTGC+TGG | 0.447799 | 3.2:-66041602 | MS.gene29111:CDS |
| AAAGGAAAGAACAATGGTTA+TGG | 0.453131 | 3.2:-66041643 | MS.gene29111:CDS |
| TGACACCTACAGAAGGTAAT+GGG | 0.465640 | 3.2:+66041509 | None:intergenic |
| ACTGTGCTGGAGATGCTAAC+TGG | 0.470469 | 3.2:-66041589 | MS.gene29111:CDS |
| CTGACACCTACAGAAGGTAA+TGG | 0.492606 | 3.2:+66041508 | None:intergenic |
| TTGTTGCTTTCCTCTTGTTG+TGG | 0.496286 | 3.2:-66040983 | MS.gene29111:CDS |
| AGAGAACCAATTTATCAACT+TGG | 0.519035 | 3.2:-66040887 | MS.gene29111:CDS |
| TGGGATATGCAGGTCGTGAA+AGG | 0.550867 | 3.2:-66041661 | MS.gene29111:intron |
| ACTCTGAATGCCACAACAAG+AGG | 0.567900 | 3.2:+66040973 | None:intergenic |
| AACATTCTGGATGGCCCCTG+AGG | 0.614718 | 3.2:-66041809 | None:intergenic |
| CTGCTTCGGATCAAGTGATG+TGG | 0.618337 | 3.2:-66040916 | MS.gene29111:CDS |
| GTCGTGAAAGGAAAGAACAA+TGG | 0.673328 | 3.2:-66041649 | MS.gene29111:CDS |
| AGATTGAAAGCAAACCTCAG+GGG | 0.742082 | 3.2:+66041795 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGATTATTAATTATTAGTC+TGG | - | chr3.2:66040992-66041011 | MS.gene29111:intron | 15.0% |
| !! | GACTAATAATTAATAATCAT+TGG | + | chr3.2:66040992-66041011 | None:intergenic | 15.0% |
| !!! | TGATTATTAATTATTAGTCT+GGG | - | chr3.2:66040993-66041012 | MS.gene29111:intron | 15.0% |
| ! | AAAGAAGCAGTAAGAAAATT+GGG | + | chr3.2:66041392-66041411 | None:intergenic | 25.0% |
| !! | ATATGCAATGATTTTCCTAA+TGG | + | chr3.2:66041581-66041600 | None:intergenic | 25.0% |
| AAAGGAAAGAACAATGGTTA+TGG | - | chr3.2:66041030-66041049 | MS.gene29111:intron | 30.0% | |
| AGAGAACCAATTTATCAACT+TGG | - | chr3.2:66041786-66041805 | MS.gene29111:intron | 30.0% | |
| ATTACTCTGCATATTGTTTC+TGG | - | chr3.2:66041638-66041657 | MS.gene29111:CDS | 30.0% | |
| CAAAGAAGCAGTAAGAAAAT+TGG | + | chr3.2:66041393-66041412 | None:intergenic | 30.0% | |
| CAATATTCATAATGTCCACT+CGG | - | chr3.2:66041245-66041264 | MS.gene29111:intron | 30.0% | |
| CAGTAAGAAAATTGGGAATT+TGG | + | chr3.2:66041385-66041404 | None:intergenic | 30.0% | |
| GAAAATCATTGCATATGAGT+TGG | - | chr3.2:66041585-66041604 | MS.gene29111:CDS | 30.0% | |
| GAGAAAAAATAGCAATGACA+TGG | + | chr3.2:66041145-66041164 | None:intergenic | 30.0% | |
| TAGTCTGAACTGTTTAATAG+TGG | + | chr3.2:66041351-66041370 | None:intergenic | 30.0% | |
| TATATCTTGTGTTCATGTTG+AGG | + | chr3.2:66040922-66040941 | None:intergenic | 30.0% | |
| ! | TTATGTTGTTTGCATCCATT+AGG | - | chr3.2:66041563-66041582 | MS.gene29111:CDS | 30.0% |
| !! | TAGATTTTTAAGCACTGCTT+CGG | - | chr3.2:66041743-66041762 | MS.gene29111:intron | 30.0% |
| AAGAAAAAAAAGACCAGGAC+TGG | + | chr3.2:66041280-66041299 | None:intergenic | 35.0% | |
| AAGATTGAAAGCAAACCTCA+GGG | + | chr3.2:66040882-66040901 | None:intergenic | 35.0% | |
| ACACAAGATATAGCAAAGCT+AGG | - | chr3.2:66040930-66040949 | MS.gene29111:CDS | 35.0% | |
| ACACATTCCAAATTGGAGTA+TGG | + | chr3.2:66041117-66041136 | None:intergenic | 35.0% | |
| AGACAGGAGAAAGATAATCA+GGG | + | chr3.2:66041210-66041229 | None:intergenic | 35.0% | |
| AGCAACAATTGCTGCAAAAA+TGG | + | chr3.2:66041678-66041697 | None:intergenic | 35.0% | |
| ATTATCTTTCTCCTGTCTCA+AGG | - | chr3.2:66041212-66041231 | MS.gene29111:intron | 35.0% | |
| CACAAGATATAGCAAAGCTA+GGG | - | chr3.2:66040931-66040950 | MS.gene29111:CDS | 35.0% | |
| CCAAATTCCATACTCCAATT+TGG | - | chr3.2:66041107-66041126 | MS.gene29111:intron | 35.0% | |
| CCAAATTGGAGTATGGAATT+TGG | + | chr3.2:66041110-66041129 | None:intergenic | 35.0% | |
| CGTGAAAGAAAAAAAAGACC+AGG | + | chr3.2:66041285-66041304 | None:intergenic | 35.0% | |
| TAAGATTGAAAGCAAACCTC+AGG | + | chr3.2:66040883-66040902 | None:intergenic | 35.0% | |
| TCTAATAACGTAGACGATGT+TGG | - | chr3.2:66041459-66041478 | MS.gene29111:intron | 35.0% | |
| TTATCTTTCTCCTGTCTCAA+GGG | - | chr3.2:66041213-66041232 | MS.gene29111:intron | 35.0% | |
| ! | ATTATTAGTCTGGGATATGC+AGG | - | chr3.2:66041002-66041021 | MS.gene29111:intron | 35.0% |
| !! | ACGTTGCCAAGTTGATAAAT+TGG | + | chr3.2:66041795-66041814 | None:intergenic | 35.0% |
| AACTCCAAACATCAGCTTGA+AGG | + | chr3.2:66041056-66041075 | None:intergenic | 40.0% | |
| AGATTGAAAGCAAACCTCAG+GGG | + | chr3.2:66040881-66040900 | None:intergenic | 40.0% | |
| GACTGGAATTAAAATCCGAG+TGG | + | chr3.2:66041263-66041282 | None:intergenic | 40.0% | |
| GAGACAGGAGAAAGATAATC+AGG | + | chr3.2:66041211-66041230 | None:intergenic | 40.0% | |
| GGCACATACACATTCCAAAT+TGG | + | chr3.2:66041124-66041143 | None:intergenic | 40.0% | |
| GTCGTGAAAGGAAAGAACAA+TGG | - | chr3.2:66041024-66041043 | MS.gene29111:intron | 40.0% | |
| TGACACCTACAGAAGGTAAT+GGG | + | chr3.2:66041167-66041186 | None:intergenic | 40.0% | |
| TTTCTCCCATTACCTTCTGT+AGG | - | chr3.2:66041159-66041178 | MS.gene29111:intron | 40.0% | |
| ! | CAAGCTGATGTTTGGAGTTT+GGG | - | chr3.2:66041057-66041076 | MS.gene29111:intron | 40.0% |
| ! | TCAAGCTGATGTTTGGAGTT+TGG | - | chr3.2:66041056-66041075 | MS.gene29111:intron | 40.0% |
| !! | ATTGTTTCTGGAGTTGACCT+GGG | - | chr3.2:66041650-66041669 | MS.gene29111:CDS | 40.0% |
| !! | TATTGTTTCTGGAGTTGACC+TGG | - | chr3.2:66041649-66041668 | MS.gene29111:CDS | 40.0% |
| !! | TTGTTGCTTTCCTCTTGTTG+TGG | - | chr3.2:66041690-66041709 | MS.gene29111:intron | 40.0% |
| !!! | TCGGATTTTAATTCCAGTCC+TGG | - | chr3.2:66041264-66041283 | MS.gene29111:intron | 40.0% |
| ACCTTCTGTAGGTGTCAGTA+AGG | - | chr3.2:66041170-66041189 | MS.gene29111:intron | 45.0% | |
| ACTCTGAATGCCACAACAAG+AGG | + | chr3.2:66041703-66041722 | None:intergenic | 45.0% | |
| ATGGCCTTCAAGCTGATGTT+TGG | - | chr3.2:66041049-66041068 | MS.gene29111:intron | 45.0% | |
| CCTTCTGTAGGTGTCAGTAA+GGG | - | chr3.2:66041171-66041190 | MS.gene29111:intron | 45.0% | |
| CTGACACCTACAGAAGGTAA+TGG | + | chr3.2:66041168-66041187 | None:intergenic | 45.0% | |
| TTGCTGCAAAAATGGAACCC+AGG | + | chr3.2:66041670-66041689 | None:intergenic | 45.0% | |
| !! | TTATATTTATATAAACAAAA+TGG | - | chr3.2:66041489-66041508 | MS.gene29111:intron | 5.0% |
| ACTGTGCTGGAGATGCTAAC+TGG | - | chr3.2:66041084-66041103 | MS.gene29111:intron | 50.0% | |
| CCCTTACTGACACCTACAGA+AGG | + | chr3.2:66041174-66041193 | None:intergenic | 50.0% | |
| CTGCTTCGGATCAAGTGATG+TGG | - | chr3.2:66041757-66041776 | MS.gene29111:intron | 50.0% | |
| TCTGTAGGTGTCAGTAAGGG+TGG | - | chr3.2:66041174-66041193 | MS.gene29111:intron | 50.0% | |
| TGGGATATGCAGGTCGTGAA+AGG | - | chr3.2:66041012-66041031 | MS.gene29111:intron | 50.0% | |
| GAAGAGCTCTCCCTTGAGAC+AGG | + | chr3.2:66041226-66041245 | None:intergenic | 55.0% | |
| ! | GAGTTTGGGATGCACTGTGC+TGG | - | chr3.2:66041071-66041090 | MS.gene29111:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 66040874 | 66041821 | 66040874 | ID=MS.gene29111 |
| chr3.2 | mRNA | 66040874 | 66041821 | 66040874 | ID=MS.gene29111.t1;Parent=MS.gene29111 |
| chr3.2 | exon | 66041810 | 66041821 | 66041810 | ID=MS.gene29111.t1.exon1;Parent=MS.gene29111.t1 |
| chr3.2 | CDS | 66041810 | 66041821 | 66041810 | ID=cds.MS.gene29111.t1;Parent=MS.gene29111.t1 |
| chr3.2 | exon | 66041519 | 66041671 | 66041519 | ID=MS.gene29111.t1.exon2;Parent=MS.gene29111.t1 |
| chr3.2 | CDS | 66041519 | 66041671 | 66041519 | ID=cds.MS.gene29111.t1;Parent=MS.gene29111.t1 |
| chr3.2 | exon | 66040874 | 66041008 | 66040874 | ID=MS.gene29111.t1.exon3;Parent=MS.gene29111.t1 |
| chr3.2 | CDS | 66040874 | 66041008 | 66040874 | ID=cds.MS.gene29111.t1;Parent=MS.gene29111.t1 |
| Gene Sequence |
| Protein sequence |
