Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29135.t1 | GAU23335.1 | 88.5 | 209 | 23 | 1 | 1 | 208 | 8 | 216 | 1.50E-92 | 349 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29135.t1 | P0CB25 | 45.1 | 204 | 95 | 4 | 1 | 197 | 42 | 235 | 1.4e-33 | 144.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29135.t1 | A0A2Z6MJD4 | 88.5 | 209 | 23 | 1 | 1 | 208 | 8 | 216 | 1.1e-92 | 349.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene29135.t1 | TF | bHLH |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29135.t1 | MTR_5g032375 | 87.442 | 215 | 19 | 2 | 1 | 208 | 1 | 214 | 1.45e-134 | 376 |
| MS.gene29135.t1 | MTR_1g093750 | 35.928 | 167 | 89 | 3 | 43 | 198 | 156 | 315 | 2.44e-24 | 98.2 |
| MS.gene29135.t1 | MTR_5g066080 | 39.130 | 161 | 77 | 2 | 45 | 184 | 37 | 197 | 1.26e-23 | 94.7 |
| MS.gene29135.t1 | MTR_3g070970 | 35.928 | 167 | 91 | 3 | 45 | 198 | 73 | 236 | 3.02e-23 | 94.0 |
| MS.gene29135.t1 | MTR_2g015890 | 37.589 | 141 | 80 | 2 | 34 | 173 | 56 | 189 | 9.92e-22 | 89.7 |
| MS.gene29135.t1 | MTR_2g091190 | 34.270 | 178 | 100 | 4 | 6 | 177 | 22 | 188 | 4.86e-21 | 87.8 |
| MS.gene29135.t1 | MTR_4g079760 | 35.220 | 159 | 86 | 3 | 34 | 185 | 57 | 205 | 1.53e-18 | 81.3 |
| MS.gene29135.t1 | MTR_2g015890 | 40.000 | 120 | 64 | 2 | 34 | 152 | 56 | 168 | 2.81e-18 | 79.7 |
| MS.gene29135.t1 | MTR_4g087920 | 36.364 | 143 | 78 | 4 | 39 | 175 | 68 | 203 | 1.84e-12 | 65.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29135.t1 | AT4G38070 | 45.596 | 193 | 84 | 5 | 1 | 184 | 42 | 222 | 5.57e-46 | 152 |
| MS.gene29135.t1 | AT3G25710 | 42.029 | 138 | 70 | 3 | 43 | 177 | 130 | 260 | 4.73e-25 | 100 |
| MS.gene29135.t1 | AT1G68810 | 40.828 | 169 | 80 | 3 | 43 | 198 | 172 | 333 | 1.74e-24 | 99.4 |
| MS.gene29135.t1 | AT3G56770 | 33.333 | 174 | 93 | 6 | 40 | 200 | 39 | 202 | 2.17e-22 | 91.3 |
| MS.gene29135.t1 | AT2G41130 | 36.994 | 173 | 91 | 5 | 39 | 200 | 60 | 225 | 2.47e-20 | 86.3 |
| MS.gene29135.t1 | AT2G40200 | 37.500 | 136 | 67 | 4 | 46 | 172 | 64 | 190 | 8.02e-20 | 84.7 |
Find 53 sgRNAs with CRISPR-Local
Find 60 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCTGCTTCACTATGTTTCT+TGG | 0.145261 | 5.3:-21489931 | None:intergenic |
| TGGGGCGGACAAATTGAATT+TGG | 0.240537 | 5.3:+21490384 | MS.gene29135:CDS |
| TGTCCGCCCCACTAGGAAAC+TGG | 0.364856 | 5.3:-21490372 | None:intergenic |
| TTGGACCCATAACACACTTC+TGG | 0.378699 | 5.3:-21490551 | None:intergenic |
| GCACTAACTTTGATCAAGTT+GGG | 0.385066 | 5.3:-21490008 | None:intergenic |
| AGGTGTTGAAAATGAAGGTT+TGG | 0.393224 | 5.3:+21490573 | MS.gene29135:CDS |
| AATGAAAGCCAAAGTTGTTA+AGG | 0.399548 | 5.3:+21490501 | MS.gene29135:CDS |
| GAAGAACCAGAAGTGTGTTA+TGG | 0.408049 | 5.3:+21490545 | MS.gene29135:CDS |
| CATCTCAACCTTAACAACTT+TGG | 0.408942 | 5.3:-21490509 | None:intergenic |
| GGCACTAACTTTGATCAAGT+TGG | 0.420765 | 5.3:-21490009 | None:intergenic |
| AAGAACCAGAAGTGTGTTAT+GGG | 0.427563 | 5.3:+21490546 | MS.gene29135:CDS |
| CTATAACGCAGAAGCTCAAA+TGG | 0.441129 | 5.3:+21489815 | MS.gene29135:CDS |
| GATGAGGCGGTATACTCAAT+AGG | 0.446628 | 5.3:+21490645 | MS.gene29135:CDS |
| GGTGTTGAAAATGAAGGTTT+GGG | 0.450241 | 5.3:+21490574 | MS.gene29135:CDS |
| GATATAGATGACATTAATCC+TGG | 0.453149 | 5.3:-21490463 | None:intergenic |
| GATAGCCATGGTACCTCAAA+GGG | 0.466904 | 5.3:+21490346 | MS.gene29135:CDS |
| GTTTGCGAGATGAGTGAACT+TGG | 0.483401 | 5.3:-21489852 | None:intergenic |
| TACCGCCTCATCTTAAAGCT+TGG | 0.493542 | 5.3:-21490634 | None:intergenic |
| TTGAGTTGTGAGGATAGGCC+AGG | 0.504463 | 5.3:+21490445 | MS.gene29135:CDS |
| TTCAATTTGTCCGCCCCACT+AGG | 0.507536 | 5.3:-21490379 | None:intergenic |
| AGACGTAGAATGCGAATCAA+TGG | 0.510341 | 5.3:+21489957 | MS.gene29135:CDS |
| GGAGACTATCAAACAAGTGC+AGG | 0.527796 | 5.3:+21490291 | MS.gene29135:CDS |
| CAAAGTTGTTAAGGTTGAGA+TGG | 0.542500 | 5.3:+21490510 | MS.gene29135:CDS |
| GTCCAAGGTGTTGAAAATGA+AGG | 0.548376 | 5.3:+21490568 | MS.gene29135:CDS |
| AGGAAACTGGATCCCCTTTG+AGG | 0.550743 | 5.3:-21490359 | None:intergenic |
| CTACATTGAGTTGTGAGGAT+AGG | 0.570986 | 5.3:+21490440 | MS.gene29135:CDS |
| GGAAAAGTGTAATGATGAGG+AGG | 0.576454 | 5.3:+21490405 | MS.gene29135:CDS |
| AGATAGCCATGGTACCTCAA+AGG | 0.576593 | 5.3:+21490345 | MS.gene29135:CDS |
| AGGGGATCCAGTTTCCTAGT+GGG | 0.580878 | 5.3:+21490365 | MS.gene29135:CDS |
| CACTAACTTTGATCAAGTTG+GGG | 0.582031 | 5.3:-21490007 | None:intergenic |
| AGAAGTGTGTTATGGGTCCA+AGG | 0.587934 | 5.3:+21490553 | MS.gene29135:CDS |
| TCAAGTTGGGGAGGATATTG+CGG | 0.598694 | 5.3:-21489995 | None:intergenic |
| GTTGAGATGGTGACAGTGGG+TGG | 0.609342 | 5.3:+21490523 | MS.gene29135:CDS |
| GAAAAGTGTAATGATGAGGA+GGG | 0.610839 | 5.3:+21490406 | MS.gene29135:CDS |
| TAACTTTGATCAAGTTGGGG+AGG | 0.613215 | 5.3:-21490004 | None:intergenic |
| TAAGGTTGAGATGGTGACAG+TGG | 0.619246 | 5.3:+21490519 | MS.gene29135:CDS |
| TTTCTCGAAGCAAAGATCAA+AGG | 0.627837 | 5.3:+21489887 | MS.gene29135:CDS |
| GGACAAGGCATCAATACTAG+TGG | 0.628919 | 5.3:+21490270 | MS.gene29135:CDS |
| GGATCCCCTTTGAGGTACCA+TGG | 0.636586 | 5.3:-21490351 | None:intergenic |
| GGGGATCCAGTTTCCTAGTG+GGG | 0.644968 | 5.3:+21490366 | MS.gene29135:CDS |
| AAGGTTGAGATGGTGACAGT+GGG | 0.645311 | 5.3:+21490520 | MS.gene29135:CDS |
| CTCGAAGCAAAGATCAAAGG+CGG | 0.646476 | 5.3:+21489890 | MS.gene29135:CDS |
| AAGGGGATCCAGTTTCCTAG+TGG | 0.647720 | 5.3:+21490364 | MS.gene29135:CDS |
| AGCCAAGCTTTAAGATGAGG+CGG | 0.655183 | 5.3:+21490632 | MS.gene29135:CDS |
| ATAAGCCAAGCTTTAAGATG+AGG | 0.659617 | 5.3:+21490629 | MS.gene29135:CDS |
| AAGCTAGAAGAAGATAGCCA+TGG | 0.662475 | 5.3:+21490334 | MS.gene29135:CDS |
| TATTGATTAACAGAAGGACA+AGG | 0.666615 | 5.3:+21490255 | MS.gene29135:intron |
| AGTTGGGGAGGATATTGCGG+AGG | 0.667078 | 5.3:-21489992 | None:intergenic |
| TTTGGAAAAGTGTAATGATG+AGG | 0.678742 | 5.3:+21490402 | MS.gene29135:CDS |
| GATCCAGTTTCCTAGTGGGG+CGG | 0.686927 | 5.3:+21490369 | MS.gene29135:CDS |
| GAAAGCTACATTGAGTTGTG+AGG | 0.689461 | 5.3:+21490435 | MS.gene29135:CDS |
| GTTGGGGAGGATATTGCGGA+GGG | 0.697366 | 5.3:-21489991 | None:intergenic |
| ATAGCCATGGTACCTCAAAG+GGG | 0.750021 | 5.3:+21490347 | MS.gene29135:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AACAGTTAAATATTTTGATA+TGG | + | chr5.3:21490142-21490161 | MS.gene29135:intron | 15.0% |
| !!! | GAAATTTTGTTTTATAAAGT+TGG | - | chr5.3:21490052-21490071 | None:intergenic | 15.0% |
| !!! | TTTGATATGGTTTTTAATTT+TGG | + | chr5.3:21490155-21490174 | MS.gene29135:intron | 15.0% |
| !!! | ATTTGTTATTGATTAACAGA+AGG | + | chr5.3:21490249-21490268 | MS.gene29135:intron | 20.0% |
| !!! | TTGGTTTAATTTTTCATGAA+TGG | - | chr5.3:21490033-21490052 | None:intergenic | 20.0% |
| !! | TAAATTTAGCAAGCTTGTTT+AGG | - | chr5.3:21490227-21490246 | None:intergenic | 25.0% |
| AATGAAAGCCAAAGTTGTTA+AGG | + | chr5.3:21490501-21490520 | MS.gene29135:CDS | 30.0% | |
| GATATAGATGACATTAATCC+TGG | - | chr5.3:21490466-21490485 | None:intergenic | 30.0% | |
| ! | TATTGATTAACAGAAGGACA+AGG | + | chr5.3:21490255-21490274 | MS.gene29135:intron | 30.0% |
| ! | TTTGGAAAAGTGTAATGATG+AGG | + | chr5.3:21490402-21490421 | MS.gene29135:CDS | 30.0% |
| CAAAGTTGTTAAGGTTGAGA+TGG | + | chr5.3:21490510-21490529 | MS.gene29135:CDS | 35.0% | |
| CATCTCAACCTTAACAACTT+TGG | - | chr5.3:21490512-21490531 | None:intergenic | 35.0% | |
| GCACTAACTTTGATCAAGTT+GGG | - | chr5.3:21490011-21490030 | None:intergenic | 35.0% | |
| TCGCGATCACTATTATTGTA+AGG | + | chr5.3:21490116-21490135 | MS.gene29135:intron | 35.0% | |
| TTCTGCTTCACTATGTTTCT+TGG | - | chr5.3:21489934-21489953 | None:intergenic | 35.0% | |
| TTTCTCGAAGCAAAGATCAA+AGG | + | chr5.3:21489887-21489906 | MS.gene29135:CDS | 35.0% | |
| ! | AACCTTCATTTTCAACACCT+TGG | - | chr5.3:21490573-21490592 | None:intergenic | 35.0% |
| ! | AAGAACCAGAAGTGTGTTAT+GGG | + | chr5.3:21490546-21490565 | MS.gene29135:CDS | 35.0% |
| ! | CACTAACTTTGATCAAGTTG+GGG | - | chr5.3:21490010-21490029 | None:intergenic | 35.0% |
| ! | GAAAAGTGTAATGATGAGGA+GGG | + | chr5.3:21490406-21490425 | MS.gene29135:CDS | 35.0% |
| ! | GGTGTTGAAAATGAAGGTTT+GGG | + | chr5.3:21490574-21490593 | MS.gene29135:CDS | 35.0% |
| !! | AGGTGTTGAAAATGAAGGTT+TGG | + | chr5.3:21490573-21490592 | MS.gene29135:CDS | 35.0% |
| !! | ATAAGCCAAGCTTTAAGATG+AGG | + | chr5.3:21490629-21490648 | MS.gene29135:CDS | 35.0% |
| AAGCTAGAAGAAGATAGCCA+TGG | + | chr5.3:21490334-21490353 | MS.gene29135:CDS | 40.0% | |
| AGACGTAGAATGCGAATCAA+TGG | + | chr5.3:21489957-21489976 | MS.gene29135:CDS | 40.0% | |
| CTATAACGCAGAAGCTCAAA+TGG | + | chr5.3:21489815-21489834 | MS.gene29135:CDS | 40.0% | |
| GGCACTAACTTTGATCAAGT+TGG | - | chr5.3:21490012-21490031 | None:intergenic | 40.0% | |
| ! | CTACATTGAGTTGTGAGGAT+AGG | + | chr5.3:21490440-21490459 | MS.gene29135:CDS | 40.0% |
| ! | GAAAGCTACATTGAGTTGTG+AGG | + | chr5.3:21490435-21490454 | MS.gene29135:CDS | 40.0% |
| ! | GAAGAACCAGAAGTGTGTTA+TGG | + | chr5.3:21490545-21490564 | MS.gene29135:CDS | 40.0% |
| ! | GGAAAAGTGTAATGATGAGG+AGG | + | chr5.3:21490405-21490424 | MS.gene29135:CDS | 40.0% |
| !! | GTCCAAGGTGTTGAAAATGA+AGG | + | chr5.3:21490568-21490587 | MS.gene29135:CDS | 40.0% |
| !! | TAACTTTGATCAAGTTGGGG+AGG | - | chr5.3:21490007-21490026 | None:intergenic | 40.0% |
| AAGGTTGAGATGGTGACAGT+GGG | + | chr5.3:21490520-21490539 | MS.gene29135:CDS | 45.0% | |
| AGATAGCCATGGTACCTCAA+AGG | + | chr5.3:21490345-21490364 | MS.gene29135:CDS | 45.0% | |
| ATAGCCATGGTACCTCAAAG+GGG | + | chr5.3:21490347-21490366 | MS.gene29135:CDS | 45.0% | |
| CTCGAAGCAAAGATCAAAGG+CGG | + | chr5.3:21489890-21489909 | MS.gene29135:CDS | 45.0% | |
| GATAGCCATGGTACCTCAAA+GGG | + | chr5.3:21490346-21490365 | MS.gene29135:CDS | 45.0% | |
| GGAGACTATCAAACAAGTGC+AGG | + | chr5.3:21490291-21490310 | MS.gene29135:CDS | 45.0% | |
| GTTTGCGAGATGAGTGAACT+TGG | - | chr5.3:21489855-21489874 | None:intergenic | 45.0% | |
| TAAGGTTGAGATGGTGACAG+TGG | + | chr5.3:21490519-21490538 | MS.gene29135:CDS | 45.0% | |
| TACCGCCTCATCTTAAAGCT+TGG | - | chr5.3:21490637-21490656 | None:intergenic | 45.0% | |
| TGGGGCGGACAAATTGAATT+TGG | + | chr5.3:21490384-21490403 | MS.gene29135:CDS | 45.0% | |
| TTGGACCCATAACACACTTC+TGG | - | chr5.3:21490554-21490573 | None:intergenic | 45.0% | |
| ! | AGAAGTGTGTTATGGGTCCA+AGG | + | chr5.3:21490553-21490572 | MS.gene29135:CDS | 45.0% |
| ! | GGACAAGGCATCAATACTAG+TGG | + | chr5.3:21490270-21490289 | MS.gene29135:CDS | 45.0% |
| !! | AGCCAAGCTTTAAGATGAGG+CGG | + | chr5.3:21490632-21490651 | MS.gene29135:CDS | 45.0% |
| !! | TCAAGTTGGGGAGGATATTG+CGG | - | chr5.3:21489998-21490017 | None:intergenic | 45.0% |
| AAGGGGATCCAGTTTCCTAG+TGG | + | chr5.3:21490364-21490383 | MS.gene29135:CDS | 50.0% | |
| AGGAAACTGGATCCCCTTTG+AGG | - | chr5.3:21490362-21490381 | None:intergenic | 50.0% | |
| AGGGGATCCAGTTTCCTAGT+GGG | + | chr5.3:21490365-21490384 | MS.gene29135:CDS | 50.0% | |
| TTCAATTTGTCCGCCCCACT+AGG | - | chr5.3:21490382-21490401 | None:intergenic | 50.0% | |
| ! | TTGAGTTGTGAGGATAGGCC+AGG | + | chr5.3:21490445-21490464 | MS.gene29135:CDS | 50.0% |
| AGTTGGGGAGGATATTGCGG+AGG | - | chr5.3:21489995-21490014 | None:intergenic | 55.0% | |
| GATCCAGTTTCCTAGTGGGG+CGG | + | chr5.3:21490369-21490388 | MS.gene29135:CDS | 55.0% | |
| GGGGATCCAGTTTCCTAGTG+GGG | + | chr5.3:21490366-21490385 | MS.gene29135:CDS | 55.0% | |
| GTTGAGATGGTGACAGTGGG+TGG | + | chr5.3:21490523-21490542 | MS.gene29135:CDS | 55.0% | |
| GTTGGGGAGGATATTGCGGA+GGG | - | chr5.3:21489994-21490013 | None:intergenic | 55.0% | |
| !! | GGATCCCCTTTGAGGTACCA+TGG | - | chr5.3:21490354-21490373 | None:intergenic | 55.0% |
| TGTCCGCCCCACTAGGAAAC+TGG | - | chr5.3:21490375-21490394 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 21489795 | 21490666 | 21489795 | ID=MS.gene29135 |
| chr5.3 | mRNA | 21489795 | 21490666 | 21489795 | ID=MS.gene29135.t1;Parent=MS.gene29135 |
| chr5.3 | exon | 21489795 | 21490022 | 21489795 | ID=MS.gene29135.t1.exon1;Parent=MS.gene29135.t1 |
| chr5.3 | CDS | 21489795 | 21490022 | 21489795 | ID=cds.MS.gene29135.t1;Parent=MS.gene29135.t1 |
| chr5.3 | exon | 21490268 | 21490666 | 21490268 | ID=MS.gene29135.t1.exon2;Parent=MS.gene29135.t1 |
| chr5.3 | CDS | 21490268 | 21490666 | 21490268 | ID=cds.MS.gene29135.t1;Parent=MS.gene29135.t1 |
| Gene Sequence |
| Protein sequence |
