Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29471.t1 | XP_024640742.1 | 91.3 | 173 | 9 | 1 | 1 | 173 | 1 | 167 | 7.70E-61 | 243.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29471.t1 | Q3EAL1 | 59.4 | 64 | 26 | 0 | 98 | 161 | 67 | 130 | 5.5e-15 | 82.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29471.t1 | A0A396HWM6 | 91.3 | 173 | 9 | 1 | 1 | 173 | 1 | 167 | 5.6e-61 | 243.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene29471.t1 | TF | OFP |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29471.t1 | MTR_5g079710 | 95.322 | 171 | 2 | 2 | 1 | 171 | 1 | 165 | 6.90e-115 | 326 |
| MS.gene29471.t1 | MTR_4g052040 | 47.024 | 168 | 79 | 4 | 6 | 166 | 4 | 168 | 6.97e-40 | 133 |
| MS.gene29471.t1 | MTR_4g052060 | 41.667 | 168 | 91 | 4 | 3 | 167 | 1 | 164 | 2.24e-33 | 117 |
| MS.gene29471.t1 | MTR_7g105790 | 36.842 | 171 | 93 | 4 | 6 | 161 | 4 | 174 | 1.13e-23 | 92.8 |
| MS.gene29471.t1 | MTR_0907s0020 | 59.420 | 69 | 28 | 0 | 97 | 165 | 102 | 170 | 1.17e-23 | 92.0 |
| MS.gene29471.t1 | MTR_8g103520 | 40.230 | 87 | 52 | 0 | 80 | 166 | 207 | 293 | 2.50e-18 | 80.5 |
| MS.gene29471.t1 | MTR_3g111780 | 50.725 | 69 | 34 | 0 | 94 | 162 | 195 | 263 | 1.10e-12 | 64.7 |
| MS.gene29471.t1 | MTR_5g075210 | 39.189 | 74 | 45 | 0 | 88 | 161 | 271 | 344 | 1.53e-11 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29471.t1 | AT3G52525 | 60.317 | 63 | 25 | 0 | 98 | 160 | 67 | 129 | 3.47e-21 | 85.1 |
| MS.gene29471.t1 | AT5G19650 | 53.030 | 66 | 31 | 0 | 97 | 162 | 154 | 219 | 7.37e-21 | 85.9 |
| MS.gene29471.t1 | AT2G36026 | 60.656 | 61 | 24 | 0 | 102 | 162 | 91 | 151 | 7.79e-20 | 82.4 |
| MS.gene29471.t1 | AT2G18500 | 48.387 | 62 | 32 | 0 | 95 | 156 | 224 | 285 | 9.14e-17 | 76.6 |
| MS.gene29471.t1 | AT5G22240 | 45.205 | 73 | 40 | 0 | 91 | 163 | 90 | 162 | 1.54e-15 | 71.2 |
| MS.gene29471.t1 | AT1G06920 | 40.580 | 69 | 41 | 0 | 94 | 162 | 240 | 308 | 7.26e-13 | 65.5 |
Find 32 sgRNAs with CRISPR-Local
Find 41 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTGGGAGAAGTGAGGGTATT+AGG | 0.156150 | 5.3:+64443371 | None:intergenic |
| CCATCACCACAAAGCTTCTT+TGG | 0.206235 | 5.3:+64443026 | None:intergenic |
| GGGTATTAGGGTTTGTGTTT+TGG | 0.265400 | 5.3:+64443384 | None:intergenic |
| CTCTATACTTCAAATGATAT+TGG | 0.278958 | 5.3:-64443171 | MS.gene29471:CDS |
| TGGGAGAAGTGAGGGTATTA+GGG | 0.326639 | 5.3:+64443372 | None:intergenic |
| TGTCATTGTTGAAGCGTTCA+TGG | 0.333768 | 5.3:-64443069 | MS.gene29471:CDS |
| GCAACAATAATAATAATTAT+AGG | 0.367952 | 5.3:-64443274 | MS.gene29471:CDS |
| AGAGGGTTCAAGTACGTTAG+AGG | 0.399454 | 5.3:+64443430 | None:intergenic |
| ATTGGAGAGAGAGATTTATT+CGG | 0.406374 | 5.3:-64443153 | MS.gene29471:CDS |
| TTTGTCGTCGGTGTTGGCTT+CGG | 0.409902 | 5.3:+64443334 | None:intergenic |
| CCAAAGAAGCTTTGTGGTGA+TGG | 0.448958 | 5.3:-64443026 | MS.gene29471:CDS |
| GAAATCTTTGTCGTCGGTGT+TGG | 0.452991 | 5.3:+64443328 | None:intergenic |
| ATATTTCCAAAGAAGCTTTG+TGG | 0.483730 | 5.3:-64443032 | MS.gene29471:CDS |
| TTCGGCGTCGTGTGAAGTGG+TGG | 0.519237 | 5.3:+64443352 | None:intergenic |
| TTCTCATCTAACAAAAGTTG+TGG | 0.528866 | 5.3:-64443470 | MS.gene29471:CDS |
| GAGTTTCGAAAATCTTCATA+CGG | 0.557400 | 5.3:+64443191 | None:intergenic |
| AGATAGCATTGCCGTTGAGA+AGG | 0.559747 | 5.3:-64443225 | MS.gene29471:CDS |
| GAGGGTTCAAGTACGTTAGA+GGG | 0.563551 | 5.3:+64443431 | None:intergenic |
| TCGGCGTCGTGTGAAGTGGT+GGG | 0.572494 | 5.3:+64443353 | None:intergenic |
| GCCGTTGTCGTTCGAGATGG+TGG | 0.578742 | 5.3:+64443298 | None:intergenic |
| TGCCGTTGAGAAGGAATCGA+AGG | 0.586299 | 5.3:-64443216 | MS.gene29471:CDS |
| GGCTTCGGCGTCGTGTGAAG+TGG | 0.589292 | 5.3:+64443349 | None:intergenic |
| GTTGTCGTTCGAGATGGTGG+TGG | 0.591904 | 5.3:+64443301 | None:intergenic |
| GCTGCCGTTGTCGTTCGAGA+TGG | 0.601950 | 5.3:+64443295 | None:intergenic |
| GACGTGTGAAGGACATGTGA+AGG | 0.602789 | 5.3:-64443001 | None:intergenic |
| GTGAAGTGGTGGGAGAAGTG+AGG | 0.604745 | 5.3:+64443363 | None:intergenic |
| TGAAGTGGTGGGAGAAGTGA+GGG | 0.621503 | 5.3:+64443364 | None:intergenic |
| ACCACCATCTCGAACGACAA+CGG | 0.631485 | 5.3:-64443299 | MS.gene29471:CDS |
| CTCCTTCGATTCCTTCTCAA+CGG | 0.636372 | 5.3:+64443214 | None:intergenic |
| ACGCTTCAACAATGACATGA+TGG | 0.645246 | 5.3:+64443075 | None:intergenic |
| GGAAGTGAAATCTTTGTCGT+CGG | 0.660486 | 5.3:+64443322 | None:intergenic |
| TGGTGATGGTAGACGTGTGA+AGG | 0.668871 | 5.3:-64443012 | MS.gene29471:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GCAACAATAATAATAATTAT+AGG | - | chr5.3:64443242-64443261 | MS.gene29471:CDS | 15.0% |
| !!! | TAATTATAGGATTTTAAAGC+CGG | - | chr5.3:64443255-64443274 | MS.gene29471:CDS | 20.0% |
| ! | CTCTATACTTCAAATGATAT+TGG | - | chr5.3:64443345-64443364 | MS.gene29471:CDS | 25.0% |
| !! | AACTTTTGTTAGATGAGAAA+AGG | + | chr5.3:64443045-64443064 | None:intergenic | 25.0% |
| !! | ACTTTTGTTAGATGAGAAAA+GGG | + | chr5.3:64443044-64443063 | None:intergenic | 25.0% |
| !!! | AATTATAGGATTTTAAAGCC+GGG | - | chr5.3:64443256-64443275 | MS.gene29471:CDS | 25.0% |
| !!! | TTTTGGTTAAAGGAGATTTT+AGG | + | chr5.3:64443118-64443137 | None:intergenic | 25.0% |
| ATATTTCCAAAGAAGCTTTG+TGG | - | chr5.3:64443484-64443503 | MS.gene29471:CDS | 30.0% | |
| ATTGGAGAGAGAGATTTATT+CGG | - | chr5.3:64443363-64443382 | MS.gene29471:CDS | 30.0% | |
| GAGTTTCGAAAATCTTCATA+CGG | + | chr5.3:64443328-64443347 | None:intergenic | 30.0% | |
| TTCTCATCTAACAAAAGTTG+TGG | - | chr5.3:64443046-64443065 | MS.gene29471:CDS | 30.0% | |
| !!! | GAGATTTTAGGTTTTTGAGA+GGG | + | chr5.3:64443106-64443125 | None:intergenic | 30.0% |
| !! | CTATCTATGAGTTTTGTACC+CGG | + | chr5.3:64443277-64443296 | None:intergenic | 35.0% |
| !!! | AGGGTTTGTGTTTTGGTTAA+AGG | + | chr5.3:64443128-64443147 | None:intergenic | 35.0% |
| !!! | GGAGATTTTAGGTTTTTGAG+AGG | + | chr5.3:64443107-64443126 | None:intergenic | 35.0% |
| ACGCTTCAACAATGACATGA+TGG | + | chr5.3:64443444-64443463 | None:intergenic | 40.0% | |
| GGAAGTGAAATCTTTGTCGT+CGG | + | chr5.3:64443197-64443216 | None:intergenic | 40.0% | |
| ! | GGGTATTAGGGTTTGTGTTT+TGG | + | chr5.3:64443135-64443154 | None:intergenic | 40.0% |
| ! | TGTCATTGTTGAAGCGTTCA+TGG | - | chr5.3:64443447-64443466 | MS.gene29471:CDS | 40.0% |
| !! | ATGAGAAAAGGGTTTTGACG+AGG | + | chr5.3:64443033-64443052 | None:intergenic | 40.0% |
| AGAGGGTTCAAGTACGTTAG+AGG | + | chr5.3:64443089-64443108 | None:intergenic | 45.0% | |
| AGATAGCATTGCCGTTGAGA+AGG | - | chr5.3:64443291-64443310 | MS.gene29471:CDS | 45.0% | |
| CCATCACCACAAAGCTTCTT+TGG | + | chr5.3:64443493-64443512 | None:intergenic | 45.0% | |
| CTCCTTCGATTCCTTCTCAA+CGG | + | chr5.3:64443305-64443324 | None:intergenic | 45.0% | |
| GAAATCTTTGTCGTCGGTGT+TGG | + | chr5.3:64443191-64443210 | None:intergenic | 45.0% | |
| GAGGGTTCAAGTACGTTAGA+GGG | + | chr5.3:64443088-64443107 | None:intergenic | 45.0% | |
| ! | CCAAAGAAGCTTTGTGGTGA+TGG | - | chr5.3:64443490-64443509 | MS.gene29471:CDS | 45.0% |
| !! | TGGGAGAAGTGAGGGTATTA+GGG | + | chr5.3:64443147-64443166 | None:intergenic | 45.0% |
| ACCACCATCTCGAACGACAA+CGG | - | chr5.3:64443217-64443236 | MS.gene29471:CDS | 50.0% | |
| TGCCGTTGAGAAGGAATCGA+AGG | - | chr5.3:64443300-64443319 | MS.gene29471:CDS | 50.0% | |
| ! | TGAAGTGGTGGGAGAAGTGA+GGG | + | chr5.3:64443155-64443174 | None:intergenic | 50.0% |
| !! | GTGGGAGAAGTGAGGGTATT+AGG | + | chr5.3:64443148-64443167 | None:intergenic | 50.0% |
| !! | TGGTGATGGTAGACGTGTGA+AGG | - | chr5.3:64443504-64443523 | MS.gene29471:CDS | 50.0% |
| !! | TTTGTCGTCGGTGTTGGCTT+CGG | + | chr5.3:64443185-64443204 | None:intergenic | 50.0% |
| ! | GTGAAGTGGTGGGAGAAGTG+AGG | + | chr5.3:64443156-64443175 | None:intergenic | 55.0% |
| ! | GTTGTCGTTCGAGATGGTGG+TGG | + | chr5.3:64443218-64443237 | None:intergenic | 55.0% |
| GCCGTTGTCGTTCGAGATGG+TGG | + | chr5.3:64443221-64443240 | None:intergenic | 60.0% | |
| GCTGCCGTTGTCGTTCGAGA+TGG | + | chr5.3:64443224-64443243 | None:intergenic | 60.0% | |
| TCGGCGTCGTGTGAAGTGGT+GGG | + | chr5.3:64443166-64443185 | None:intergenic | 60.0% | |
| TTCGGCGTCGTGTGAAGTGG+TGG | + | chr5.3:64443167-64443186 | None:intergenic | 60.0% | |
| GGCTTCGGCGTCGTGTGAAG+TGG | + | chr5.3:64443170-64443189 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 64443004 | 64443534 | 64443004 | ID=MS.gene29471 |
| chr5.3 | mRNA | 64443004 | 64443534 | 64443004 | ID=MS.gene29471.t1;Parent=MS.gene29471 |
| chr5.3 | exon | 64443004 | 64443534 | 64443004 | ID=MS.gene29471.t1.exon1;Parent=MS.gene29471.t1 |
| chr5.3 | CDS | 64443004 | 64443534 | 64443004 | ID=cds.MS.gene29471.t1;Parent=MS.gene29471.t1 |
| Gene Sequence |
| Protein sequence |
