AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene29480

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene29480.t1 MTR_3g005460 70.663 392 94 3 13 384 40 430 0.0 547
MS.gene29480.t1 MTR_3g005460 55.435 184 60 1 194 377 2 163 9.88e-59 199
MS.gene29480.t1 MTR_3g005460 46.025 239 79 4 13 202 277 514 1.19e-57 197
MS.gene29480.t1 MTR_3g005420 67.164 402 102 3 13 384 55 456 1.66e-177 506
MS.gene29480.t1 MTR_3g005420 48.918 231 76 2 13 202 304 533 1.62e-62 210
MS.gene29480.t1 MTR_3g005420 54.023 174 57 3 212 384 35 186 3.44e-49 174
MS.gene29480.t1 MTR_3g005495 70.402 348 90 2 11 346 26 372 7.05e-174 494
MS.gene29480.t1 MTR_3g005495 47.926 217 82 4 13 201 258 471 7.55e-56 191
MS.gene29480.t1 MTR_3g005440 58.537 410 113 4 10 389 32 414 1.41e-148 428
MS.gene29480.t1 MTR_3g005420 59.701 402 97 4 13 384 36 402 2.99e-147 427
MS.gene29480.t1 MTR_3g005420 54.023 174 57 3 212 384 16 167 1.05e-49 175
MS.gene29480.t1 MTR_3g005420 46.429 196 98 2 13 202 285 479 5.72e-43 157
MS.gene29480.t1 MTR_5g070440 73.988 173 45 0 212 384 16 188 6.94e-87 265
MS.gene29480.t1 MTR_5g070440 53.247 231 66 2 13 202 36 265 2.23e-73 230
MS.gene29480.t1 MTR_3g005490 67.630 173 46 1 212 384 16 178 1.10e-78 244
MS.gene29480.t1 MTR_3g005490 52.632 228 75 3 13 208 36 262 5.23e-70 221
MS.gene29480.t1 MTR_3g005480 66.474 173 48 1 212 384 16 178 3.53e-76 237
MS.gene29480.t1 MTR_3g005480 51.293 232 79 3 4 202 27 257 1.52e-67 215
MS.gene29480.t1 MTR_1g034240 45.455 99 54 0 217 315 7 105 1.88e-25 107
MS.gene29480.t1 MTR_1g034240 41.935 93 54 0 4 96 13 105 1.83e-19 90.1
MS.gene29480.t1 MTR_1g015350 41.379 145 54 2 42 186 59 172 1.31e-22 94.4
MS.gene29480.t1 MTR_1g015350 40.426 141 57 4 260 399 58 172 4.10e-19 84.7
MS.gene29480.t1 MTR_4g068320 41.176 102 60 0 214 315 3 104 3.28e-21 95.1
MS.gene29480.t1 MTR_4g068320 35.577 104 67 0 1 104 9 112 3.69e-16 80.1
MS.gene29480.t1 MTR_1g034210 44.444 90 50 0 217 306 7 96 1.19e-20 92.4
MS.gene29480.t1 MTR_1g034210 41.667 84 47 1 13 96 22 103 8.73e-15 75.1
MS.gene29480.t1 MTR_1g034210 44.444 90 50 0 217 306 7 96 2.26e-20 92.0
MS.gene29480.t1 MTR_1g034210 37.500 96 58 1 1 96 10 103 1.21e-14 75.1
MS.gene29480.t1 MTR_7g050560 32.161 199 107 7 216 387 12 209 1.40e-18 86.3
MS.gene29480.t1 MTR_7g050560 34.286 105 65 1 14 114 29 133 1.52e-16 80.1
MS.gene29480.t1 MTR_3g061310 47.191 89 45 1 10 96 20 108 2.20e-18 85.1
MS.gene29480.t1 MTR_3g061310 27.717 184 104 4 217 374 12 192 2.05e-14 73.6
MS.gene29480.t1 MTR_3g061350 40.351 114 65 2 10 121 20 132 3.45e-17 81.6
MS.gene29480.t1 MTR_3g061350 32.414 145 92 4 217 359 12 152 2.24e-13 70.5
MS.gene29480.t1 MTR_7g050720 31.884 138 94 0 216 353 9 146 8.31e-17 81.3
MS.gene29480.t1 MTR_7g050720 33.333 84 56 0 14 97 26 109 3.09e-12 67.4
MS.gene29480.t1 MTR_3g098790 30.872 149 94 2 216 355 12 160 1.43e-16 80.5
MS.gene29480.t1 MTR_3g098790 34.118 85 56 0 14 98 29 113 4.93e-13 70.1
MS.gene29480.t1 MTR_7g050710 32.414 145 87 3 210 343 4 148 1.66e-16 80.1
MS.gene29480.t1 MTR_7g050710 34.579 107 62 1 13 111 28 134 6.49e-16 78.6
MS.gene29480.t1 MTR_3g061320 46.067 89 46 1 10 96 20 108 1.87e-16 79.3
MS.gene29480.t1 MTR_3g061320 32.639 144 94 2 216 359 11 151 3.12e-14 72.8
MS.gene29480.t1 MTR_3g061290 44.944 89 47 1 10 96 20 108 5.29e-16 76.6
MS.gene29480.t1 MTR_3g061290 35.043 117 73 2 216 332 11 124 4.97e-14 70.9
MS.gene29480.t1 MTR_7g050580 33.094 139 77 3 210 336 7 141 5.60e-16 78.6
MS.gene29480.t1 MTR_7g050580 30.357 112 75 2 14 124 29 138 6.56e-13 69.3
MS.gene29480.t1 MTR_3g061370 42.857 84 48 0 13 96 22 105 2.48e-15 76.3
MS.gene29480.t1 MTR_3g061370 28.191 188 105 4 214 374 6 190 1.83e-13 70.9
MS.gene29480.t1 MTR_5g044670 36.145 83 53 0 14 96 32 114 3.10e-15 76.6
MS.gene29480.t1 MTR_5g044670 31.858 113 77 0 203 315 2 114 4.50e-15 76.3
MS.gene29480.t1 MTR_3g061330 42.857 84 48 0 13 96 25 108 3.98e-15 75.1
MS.gene29480.t1 MTR_3g061330 28.649 185 101 4 217 374 12 192 3.82e-13 69.3
MS.gene29480.t1 MTR_7g050590 33.929 112 67 3 14 118 2 113 2.41e-14 73.6
MS.gene29480.t1 MTR_7g050590 32.432 111 69 1 233 337 2 112 6.94e-14 72.0
MS.gene29480.t1 MTR_3g061340 46.667 90 45 2 10 96 20 109 2.46e-14 73.2
MS.gene29480.t1 MTR_3g061340 30.890 191 111 7 217 390 12 198 8.28e-14 71.6
MS.gene29480.t1 MTR_3g061220 41.573 89 37 2 10 96 20 95 8.13e-14 68.2
MS.gene29480.t1 MTR_3g061220 35.354 99 49 2 217 315 12 95 1.25e-11 61.6
MS.gene29480.t1 MTR_1g108780 30.909 110 71 1 11 115 10 119 1.55e-13 72.4
MS.gene29480.t1 MTR_1g035460 22.047 381 251 13 14 375 2 355 1.47e-12 68.9
MS.gene29480.t1 MTR_8g103940 33.333 84 56 0 13 96 54 137 3.54e-12 68.2
MS.gene29480.t1 MTR_8g103940 25.899 139 95 1 217 347 39 177 3.82e-11 65.1
MS.gene29480.t1 MTR_7g050640 36.893 103 63 2 216 317 12 113 1.59e-11 61.6
MS.gene29480.t1 MTR_1g021290 33.684 95 62 1 216 310 27 120 5.02e-11 63.9
MS.gene29480.t1 MTR_4g119990 28.049 164 92 5 216 375 14 155 6.19e-11 63.9
MS.gene29480.t1 MTR_6g043890 31.061 132 76 4 216 338 14 139 9.23e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene29480.t1 AT3G18990 42.424 99 57 0 217 315 6 104 6.58e-20 90.5
MS.gene29480.t1 AT3G18990 41.667 84 49 0 13 96 21 104 2.13e-17 83.2
MS.gene29480.t1 AT3G18990 42.424 99 57 0 217 315 6 104 1.31e-19 90.1
MS.gene29480.t1 AT3G18990 41.667 84 49 0 13 96 21 104 4.30e-17 82.4
MS.gene29480.t1 AT4G01580 36.364 121 75 1 202 320 13 133 4.97e-19 84.7
MS.gene29480.t1 AT4G01580 34.737 95 62 0 4 98 36 130 5.25e-15 73.6
MS.gene29480.t1 AT3G18960 34.426 122 78 1 201 320 12 133 6.69e-18 82.0
MS.gene29480.t1 AT3G18960 33.684 95 63 0 4 98 36 130 3.59e-14 71.6
MS.gene29480.t1 AT3G18960 35.294 119 75 1 201 317 12 130 7.54e-18 81.6
MS.gene29480.t1 AT3G18960 33.684 95 63 0 4 98 36 130 1.90e-14 72.0
MS.gene29480.t1 AT1G49475 38.542 96 58 1 11 106 47 141 7.37e-16 75.9
MS.gene29480.t1 AT1G49475 36.036 111 70 1 215 325 32 141 1.59e-15 75.1
MS.gene29480.t1 AT3G18960 35.632 87 56 0 12 98 7 93 1.05e-13 69.3
MS.gene29480.t1 AT3G18960 34.831 89 58 0 232 320 8 96 1.08e-13 69.3
MS.gene29480.t1 AT2G24645 22.096 353 239 10 36 362 43 385 3.94e-11 64.7
MS.gene29480.t1 AT2G24645 22.427 379 255 12 12 362 26 393 5.33e-11 64.7
MS.gene29480.t1 AT2G24645 22.096 353 239 10 36 362 43 385 5.64e-11 64.7
MS.gene29480.t1 AT2G24645 22.427 379 255 12 12 362 4 371 5.85e-11 64.3

Find 81 sgRNAs with CRISPR-Local

Find 144 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAGACTTTATAACAATATT+TGG 0.103105 5.3:+64517653 MS.gene29480:CDS
ACTGCTTTCCTTGAGAATTT+TGG 0.150373 5.3:-64516693 None:intergenic
TGTCTAAATATGCTTCTAAA+AGG 0.186290 5.3:+64518005 MS.gene29480:CDS
GAGCACTTTGAACGAAAATA+AGG 0.215729 5.3:-64518064 None:intergenic
GGATGGTCTAATTTGGAAAA+TGG 0.229629 5.3:+64517712 MS.gene29480:CDS
CTGTTCCGGATGGTCTAATT+TGG 0.238913 5.3:+64517705 MS.gene29480:CDS
GATGAGTTTATAACAAGATT+TGG 0.242784 5.3:+64516215 MS.gene29480:CDS
TATGGCCTTCTTAAAATGTT+TGG 0.280661 5.3:-64517463 None:intergenic
AATATTGTTATAAAGTCTTC+TGG 0.286515 5.3:-64517650 None:intergenic
ATTTCCTATCCATTCAAAAC+TGG 0.287220 5.3:+64516449 MS.gene29480:CDS
CTGTAGTGACCAATCTTTAG+AGG 0.292480 5.3:-64516183 None:intergenic
GGACTATGACACTCTTTGTT+TGG 0.315553 5.3:-64517926 None:intergenic
TTCAAAACTCTAAGTACTAA+TGG 0.332671 5.3:+64517899 MS.gene29480:CDS
AAGTGAGAGTCAAGAGAAAA+AGG 0.347170 5.3:+64517973 MS.gene29480:CDS
GTTTATTGTGTACTGAATAA+AGG 0.348113 5.3:+64516721 MS.gene29480:CDS
GGTGAAATTTACAATTTGTT+TGG 0.353537 5.3:-64517505 None:intergenic
TACAATTTGTTTGGCATGAA+TGG 0.360092 5.3:-64517496 None:intergenic
ATGAGTTTATAACAAGATTT+GGG 0.360502 5.3:+64516216 MS.gene29480:CDS
AAGACTTTATAACAATATTT+GGG 0.361172 5.3:+64517654 MS.gene29480:CDS
TTCAAAACTGGGAAGACTAA+TGG 0.374313 5.3:+64516461 MS.gene29480:CDS
TGGAGCTTGAACAGAACATT+TGG 0.381563 5.3:-64516673 None:intergenic
CCTCACCACATATCTTCCTT+TGG 0.412092 5.3:-64518086 None:intergenic
AATCTTGTTATAAACTCATC+TGG 0.413404 5.3:-64516212 None:intergenic
TGCAGGCGGAGAAGGAAGTT+AGG 0.415021 5.3:+64517436 MS.gene29480:intron
ACTCTCACTTGTTTCGACCT+TGG 0.417847 5.3:-64517960 None:intergenic
ATGAATGGGTGACGGAAGTA+TGG 0.419901 5.3:-64517481 None:intergenic
AAAGCAATGCGAATAACGTT+TGG 0.420748 5.3:+64516751 MS.gene29480:CDS
TACTTGTCTTTCAAATATGA+AGG 0.427472 5.3:+64517818 MS.gene29480:CDS
GAACACTACTCTATCTGTTA+TGG 0.428310 5.3:+64516353 MS.gene29480:CDS
TATGTAGATTTCAAGTATGA+AGG 0.431237 5.3:+64516380 MS.gene29480:CDS
TGTTGCTACAGTTACTGTTC+CGG 0.431782 5.3:+64517691 MS.gene29480:CDS
AGCACTTTGAACGAAAATAA+GGG 0.459706 5.3:-64518063 None:intergenic
ATCACCCTTCCTCTAAAGAT+TGG 0.475124 5.3:+64516174 MS.gene29480:CDS
TGATGGTTGCGAATGGCATA+TGG 0.478132 5.3:+64516274 MS.gene29480:CDS
ACAAAGAGTGTCATAGTCCT+AGG 0.483569 5.3:+64517930 MS.gene29480:CDS
GTCTAAATATGCTTCTAAAA+GGG 0.483677 5.3:+64518006 MS.gene29480:CDS
ATATGCCATTCGCAACCATC+AGG 0.491528 5.3:-64516272 None:intergenic
TATTGAATTGCAGGCGGAGA+AGG 0.498005 5.3:+64517428 MS.gene29480:intron
ATATCGACAGTCAAAGGATT+GGG 0.499850 5.3:-64516516 None:intergenic
ACAATTTGTTTGGCATGAAT+GGG 0.501644 5.3:-64517495 None:intergenic
AAATACTATTCTGTAGGATA+CGG 0.506013 5.3:+64517791 MS.gene29480:CDS
TATTCTGCGGCGAATTCAGC+AGG 0.510693 5.3:-64516643 None:intergenic
TTGGATTCTTTAGATTTGCA+AGG 0.513403 5.3:+64516770 MS.gene29480:CDS
AGTGTCATAGTCCTAGGAAA+AGG 0.516090 5.3:+64517936 MS.gene29480:CDS
CATATCGACAGTCAAAGGAT+TGG 0.519552 5.3:-64516517 None:intergenic
AAGGATTGGGAACGAAAAGA+AGG 0.522583 5.3:-64516503 None:intergenic
GCAGGCGGAGAAGGAAGTTA+GGG 0.528822 5.3:+64517437 MS.gene29480:intron
ACTTGTCTTTCAAATATGAA+GGG 0.536470 5.3:+64517819 MS.gene29480:CDS
CACCGCATATCGACAGTCAA+AGG 0.544452 5.3:-64516522 None:intergenic
TTTCCTATCCATTCAAAACT+GGG 0.546790 5.3:+64516450 MS.gene29480:CDS
AGGGAAAACAATCTATCAGA+TGG 0.548736 5.3:+64516790 MS.gene29480:CDS
ATGTAGATTTCAAGTATGAA+GGG 0.551054 5.3:+64516381 MS.gene29480:CDS
TTTCGTTCAAAGTGCTCCAA+AGG 0.552472 5.3:+64518070 MS.gene29480:CDS
TTCAAGCTCCAAAATTCTCA+AGG 0.552648 5.3:+64516685 MS.gene29480:CDS
ATATGCTTCTAAAAGGGCTG+AGG 0.564166 5.3:+64518012 MS.gene29480:CDS
TGGATTCTTTAGATTTGCAA+GGG 0.565236 5.3:+64516771 MS.gene29480:CDS
AGTGACCAATCTTTAGAGGA+AGG 0.568653 5.3:-64516179 None:intergenic
GCCACGATTACTGTTCCTGA+TGG 0.580999 5.3:+64516257 MS.gene29480:CDS
AAAATTCTCAAGGAAAGCAG+TGG 0.599450 5.3:+64516695 MS.gene29480:CDS
ATAGAGTAGTGTTCTGCGAA+CGG 0.599484 5.3:-64516344 None:intergenic
AAAATGGAGTTGAAGATATG+TGG 0.601427 5.3:+64517728 MS.gene29480:CDS
TGTGTACTGAATAAAGGTGG+CGG 0.604963 5.3:+64516727 MS.gene29480:CDS
AGGATTGGGAACGAAAAGAA+GGG 0.605751 5.3:-64516502 None:intergenic
GTGACCAATCTTTAGAGGAA+GGG 0.612342 5.3:-64516178 None:intergenic
GTGCTCCAAAGGAAGATATG+TGG 0.623189 5.3:+64518081 MS.gene29480:CDS
ATTTGGCTTCAAATATTCTG+CGG 0.631419 5.3:-64516656 None:intergenic
ATCCTTTGACTGTCGATATG+CGG 0.632326 5.3:+64516520 MS.gene29480:CDS
GCTACAGTTACTGTTCCGGA+TGG 0.632577 5.3:+64517695 MS.gene29480:CDS
AAATTCTCAAGGAAAGCAGT+GGG 0.637805 5.3:+64516696 MS.gene29480:CDS
TATTGTGTACTGAATAAAGG+TGG 0.641337 5.3:+64516724 MS.gene29480:CDS
TAGTCCTAGGAAAAGGTCCA+AGG 0.641569 5.3:+64517943 MS.gene29480:CDS
CTGTTCCTGATGGTTGCGAA+TGG 0.641772 5.3:+64516267 MS.gene29480:CDS
GGAGTTGAAGATATGTGGCA+AGG 0.642891 5.3:+64517733 MS.gene29480:CDS
ACAAGTTACAGCTTCTCACA+CGG 0.646742 5.3:+64516861 MS.gene29480:CDS
ATGAGTTTGAGCTTATCAAG+AGG 0.652349 5.3:+64516821 MS.gene29480:CDS
ACCATCAGGAACAGTAATCG+TGG 0.663147 5.3:-64516258 None:intergenic
CCAAAGGAAGATATGTGGTG+AGG 0.663159 5.3:+64518086 MS.gene29480:CDS
TGTTTGGCATGAATGGGTGA+CGG 0.666868 5.3:-64517489 None:intergenic
CATATGGAAGTAAAGAAACG+TGG 0.701477 5.3:+64516290 MS.gene29480:CDS
GTAACTTGTAACACAACACG+CGG 0.711770 5.3:-64516847 None:intergenic
AATCACGATAGTCAACCACG+CGG 0.904290 5.3:-64518132 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGACTTTATAACAATATTT+GGG + chr5.3:64517654-64517673 MS.gene29480:CDS 15.0%
!!! ATGTTTGAGTTATATATTTA+TGG + chr5.3:64516580-64516599 MS.gene29480:intron 15.0%
!! AATATTGTTATAAAGTCTTC+TGG - chr5.3:64517653-64517672 None:intergenic 20.0%
!! ATGAGTTTATAACAAGATTT+GGG + chr5.3:64516216-64516235 MS.gene29480:CDS 20.0%
!! GAAGACTTTATAACAATATT+TGG + chr5.3:64517653-64517672 MS.gene29480:CDS 20.0%
!! TGATGTATTGTAATTCATAT+AGG + chr5.3:64516955-64516974 MS.gene29480:intron 20.0%
!!! AAGTTTTCTTTTGCAAAAAT+TGG + chr5.3:64516318-64516337 MS.gene29480:CDS 20.0%
!!! ATTGATGATAGCTATAAAAA+GGG - chr5.3:64517193-64517212 None:intergenic 20.0%
!!! GGATTTTACTATACATTATT+TGG + chr5.3:64516546-64516565 MS.gene29480:intron 20.0%
!!! TCTTTGTTTATAATGTGAAT+CGG + chr5.3:64517370-64517389 MS.gene29480:intron 20.0%
!!! TTGATGATAATATAACTCAT+CGG - chr5.3:64517600-64517619 None:intergenic 20.0%
!!! TTTTCAAAATACTATTCTGT+AGG + chr5.3:64517785-64517804 MS.gene29480:CDS 20.0%
! AAATACTATTCTGTAGGATA+CGG + chr5.3:64517791-64517810 MS.gene29480:CDS 25.0%
! ACTTGTCTTTCAAATATGAA+GGG + chr5.3:64517819-64517838 MS.gene29480:CDS 25.0%
! CTTATTACTTATTGAATTGC+AGG + chr5.3:64517419-64517438 MS.gene29480:intron 25.0%
! GATGAGTTTATAACAAGATT+TGG + chr5.3:64516215-64516234 MS.gene29480:CDS 25.0%
! GTTTATTGTGTACTGAATAA+AGG + chr5.3:64516721-64516740 MS.gene29480:CDS 25.0%
! TACTTGTCTTTCAAATATGA+AGG + chr5.3:64517818-64517837 MS.gene29480:CDS 25.0%
! TGTCTAAATATGCTTCTAAA+AGG + chr5.3:64518005-64518024 MS.gene29480:CDS 25.0%
! TTCAAAACTCTAAGTACTAA+TGG + chr5.3:64517899-64517918 MS.gene29480:CDS 25.0%
!! AATCTTGTTATAAACTCATC+TGG - chr5.3:64516215-64516234 None:intergenic 25.0%
!! ACATTTTCTTTTGCAACAAA+TGG + chr5.3:64517756-64517775 MS.gene29480:CDS 25.0%
!! ATGTAGATTTCAAGTATGAA+GGG + chr5.3:64516381-64516400 MS.gene29480:CDS 25.0%
!! CATTGATGATAGCTATAAAA+AGG - chr5.3:64517194-64517213 None:intergenic 25.0%
!! GGATTTTACTTTGTAGTTTT+TGG + chr5.3:64518107-64518126 MS.gene29480:CDS 25.0%
!! GGTGAAATTTACAATTTGTT+TGG - chr5.3:64517508-64517527 None:intergenic 25.0%
!! GTCTAAATATGCTTCTAAAA+GGG + chr5.3:64518006-64518025 MS.gene29480:CDS 25.0%
!! TATGTAGATTTCAAGTATGA+AGG + chr5.3:64516380-64516399 MS.gene29480:CDS 25.0%
!!! AACGAAAATAAGGGTTTTTT+GGG - chr5.3:64518057-64518076 None:intergenic 25.0%
!!! ATTCATATAGGTAGTTTTGT+TGG + chr5.3:64516967-64516986 MS.gene29480:intron 25.0%
!!! TTAGTACTTAGAGTTTTGAA+TGG - chr5.3:64517899-64517918 None:intergenic 25.0%
AAAATGGAGTTGAAGATATG+TGG + chr5.3:64517728-64517747 MS.gene29480:CDS 30.0%
AAGAATAATGAACTCGTATG+TGG - chr5.3:64517354-64517373 None:intergenic 30.0%
AATGGAGGAACAATCTATTT+AGG - chr5.3:64516611-64516630 None:intergenic 30.0%
AGCACTTTGAACGAAAATAA+GGG - chr5.3:64518066-64518085 None:intergenic 30.0%
ATTACTTATTGAATTGCAGG+CGG + chr5.3:64517422-64517441 MS.gene29480:intron 30.0%
ATTTCCTATCCATTCAAAAC+TGG + chr5.3:64516449-64516468 MS.gene29480:CDS 30.0%
TACAATACACCAAAAATGGT+GGG + chr5.3:64517119-64517138 MS.gene29480:intron 30.0%
TATGGCCTTCTTAAAATGTT+TGG - chr5.3:64517466-64517485 None:intergenic 30.0%
TATTGTGTACTGAATAAAGG+TGG + chr5.3:64516724-64516743 MS.gene29480:CDS 30.0%
TCTTATGTAAAAACAGGGTA+AGG + chr5.3:64517091-64517110 MS.gene29480:intron 30.0%
TGGATTCTTTAGATTTGCAA+GGG + chr5.3:64516771-64516790 MS.gene29480:CDS 30.0%
TTGGATTCTTTAGATTTGCA+AGG + chr5.3:64516770-64516789 MS.gene29480:CDS 30.0%
TTTCCTATCCATTCAAAACT+GGG + chr5.3:64516450-64516469 MS.gene29480:CDS 30.0%
! ACAATTTGTTTGGCATGAAT+GGG - chr5.3:64517498-64517517 None:intergenic 30.0%
! ATTTGGCTTCAAATATTCTG+CGG - chr5.3:64516659-64516678 None:intergenic 30.0%
! ATTTTCCAAATTAGACCATC+CGG - chr5.3:64517713-64517732 None:intergenic 30.0%
! TACAATTTGTTTGGCATGAA+TGG - chr5.3:64517499-64517518 None:intergenic 30.0%
!! GGAATCCAAACATTTTAAGA+AGG + chr5.3:64517458-64517477 MS.gene29480:CDS 30.0%
!!! AACGAAAAGAAGGGTTTTTT+GGG - chr5.3:64516496-64516515 None:intergenic 30.0%
!!! GAACGAAAATAAGGGTTTTT+TGG - chr5.3:64518058-64518077 None:intergenic 30.0%
!!! TTACCCTGTTTTTACATAAG+AGG - chr5.3:64517092-64517111 None:intergenic 30.0%
AAAATTCTCAAGGAAAGCAG+TGG + chr5.3:64516695-64516714 MS.gene29480:CDS 35.0%
AAAGCAATGCGAATAACGTT+TGG + chr5.3:64516751-64516770 MS.gene29480:CDS 35.0%
AAATTCTCAAGGAAAGCAGT+GGG + chr5.3:64516696-64516715 MS.gene29480:CDS 35.0%
AAGTGAGAGTCAAGAGAAAA+AGG + chr5.3:64517973-64517992 MS.gene29480:CDS 35.0%
ACAGCCTCTTATGTAAAAAC+AGG + chr5.3:64517085-64517104 MS.gene29480:intron 35.0%
AGCTATCATCAATGACTGAA+AGG + chr5.3:64517014-64517033 MS.gene29480:intron 35.0%
AGGGAAAACAATCTATCAGA+TGG + chr5.3:64516790-64516809 MS.gene29480:CDS 35.0%
ATATCGACAGTCAAAGGATT+GGG - chr5.3:64516519-64516538 None:intergenic 35.0%
ATGAGTTTGAGCTTATCAAG+AGG + chr5.3:64516821-64516840 MS.gene29480:CDS 35.0%
CAGCCTCTTATGTAAAAACA+GGG + chr5.3:64517086-64517105 MS.gene29480:intron 35.0%
CATATGGAAGTAAAGAAACG+TGG + chr5.3:64516290-64516309 MS.gene29480:CDS 35.0%
GAACACTACTCTATCTGTTA+TGG + chr5.3:64516353-64516372 MS.gene29480:CDS 35.0%
GAGCACTTTGAACGAAAATA+AGG - chr5.3:64518067-64518086 None:intergenic 35.0%
GCTATCATCAATGAGCAATA+TGG + chr5.3:64517201-64517220 MS.gene29480:intron 35.0%
GGATGGTCTAATTTGGAAAA+TGG + chr5.3:64517712-64517731 MS.gene29480:CDS 35.0%
GTACAATACACCAAAAATGG+TGG + chr5.3:64517118-64517137 MS.gene29480:intron 35.0%
TAGCTATAAAAAGGGCAATC+CGG - chr5.3:64517185-64517204 None:intergenic 35.0%
TGCGTACAATACACCAAAAA+TGG + chr5.3:64517115-64517134 MS.gene29480:intron 35.0%
TTCAAAACTGGGAAGACTAA+TGG + chr5.3:64516461-64516480 MS.gene29480:CDS 35.0%
TTCAAGCTCCAAAATTCTCA+AGG + chr5.3:64516685-64516704 MS.gene29480:CDS 35.0%
! ACTGCTTTCCTTGAGAATTT+TGG - chr5.3:64516696-64516715 None:intergenic 35.0%
!! TAACTCATCGGTTCTACTAT+AGG - chr5.3:64517588-64517607 None:intergenic 35.0%
!!! AAAGAAGGGTTTTTTGGGTT+TGG - chr5.3:64516491-64516510 None:intergenic 35.0%
!!! AGGTAGTTTTGTTGGTTGTT+AGG + chr5.3:64516975-64516994 MS.gene29480:intron 35.0%
!!! GAACGAAAAGAAGGGTTTTT+TGG - chr5.3:64516497-64516516 None:intergenic 35.0%
!!! TTAGTCTTCCCAGTTTTGAA+TGG - chr5.3:64516461-64516480 None:intergenic 35.0%
AAGGATTGGGAACGAAAAGA+AGG - chr5.3:64516506-64516525 None:intergenic 40.0%
ACAAGTTACAGCTTCTCACA+CGG + chr5.3:64516861-64516880 MS.gene29480:CDS 40.0%
AGCAGGTACATACTACACAA+TGG - chr5.3:64516629-64516648 None:intergenic 40.0%
AGGATTGGGAACGAAAAGAA+GGG - chr5.3:64516505-64516524 None:intergenic 40.0%
AGGTACATACTACACAATGG+AGG - chr5.3:64516626-64516645 None:intergenic 40.0%
AGTGTCATAGTCCTAGGAAA+AGG + chr5.3:64517936-64517955 MS.gene29480:CDS 40.0%
ATAGAGTAGTGTTCTGCGAA+CGG - chr5.3:64516347-64516366 None:intergenic 40.0%
ATCCTTTGACTGTCGATATG+CGG + chr5.3:64516520-64516539 MS.gene29480:CDS 40.0%
CATATCGACAGTCAAAGGAT+TGG - chr5.3:64516520-64516539 None:intergenic 40.0%
CTGTAGTGACCAATCTTTAG+AGG - chr5.3:64516186-64516205 None:intergenic 40.0%
GGACTATGACACTCTTTGTT+TGG - chr5.3:64517929-64517948 None:intergenic 40.0%
GTTGTTGTCATGTGACTGAA+AGG + chr5.3:64517041-64517060 MS.gene29480:intron 40.0%
TCATGTGACTGAAAGGTCAT+GGG + chr5.3:64517048-64517067 MS.gene29480:intron 40.0%
TGGAGCTTGAACAGAACATT+TGG - chr5.3:64516676-64516695 None:intergenic 40.0%
TGTGTACTGAATAAAGGTGG+CGG + chr5.3:64516727-64516746 MS.gene29480:CDS 40.0%
TGTTGCTACAGTTACTGTTC+CGG + chr5.3:64517691-64517710 MS.gene29480:CDS 40.0%
! AGTGACCAATCTTTAGAGGA+AGG - chr5.3:64516182-64516201 None:intergenic 40.0%
! ATATGCTTCTAAAAGGGCTG+AGG + chr5.3:64518012-64518031 MS.gene29480:CDS 40.0%
! ATCACCCTTCCTCTAAAGAT+TGG + chr5.3:64516174-64516193 MS.gene29480:CDS 40.0%
! GTAACTTGTAACACAACACG+CGG - chr5.3:64516850-64516869 None:intergenic 40.0%
! GTGACCAATCTTTAGAGGAA+GGG - chr5.3:64516181-64516200 None:intergenic 40.0%
! TTACATAAGAGGCTGTTTCC+AGG - chr5.3:64517081-64517100 None:intergenic 40.0%
! TTTCGTTCAAAGTGCTCCAA+AGG + chr5.3:64518070-64518089 MS.gene29480:CDS 40.0%
!! ACAAAGAGTGTCATAGTCCT+AGG + chr5.3:64517930-64517949 MS.gene29480:CDS 40.0%
!!! CTTCCCAGTTTTGAATGGAT+AGG - chr5.3:64516456-64516475 None:intergenic 40.0%
!!! GTTTTTTGGGCTGAATGCAT+TGG - chr5.3:64518044-64518063 None:intergenic 40.0%
AATCACGATAGTCAACCACG+CGG - chr5.3:64518135-64518154 None:intergenic 45.0%
ACCATCAGGAACAGTAATCG+TGG - chr5.3:64516261-64516280 None:intergenic 45.0%
ACTCTCACTTGTTTCGACCT+TGG - chr5.3:64517963-64517982 None:intergenic 45.0%
ATATGCCATTCGCAACCATC+AGG - chr5.3:64516275-64516294 None:intergenic 45.0%
CCAAAGGAAGATATGTGGTG+AGG + chr5.3:64518086-64518105 MS.gene29480:CDS 45.0%
CCTCACCACATATCTTCCTT+TGG - chr5.3:64518089-64518108 None:intergenic 45.0%
CTGTTCCGGATGGTCTAATT+TGG + chr5.3:64517705-64517724 MS.gene29480:CDS 45.0%
GGAGTTGAAGATATGTGGCA+AGG + chr5.3:64517733-64517752 MS.gene29480:CDS 45.0%
GTCATGTGACTGAAAGGTCA+TGG + chr5.3:64517047-64517066 MS.gene29480:intron 45.0%
GTGCTCCAAAGGAAGATATG+TGG + chr5.3:64518081-64518100 MS.gene29480:CDS 45.0%
TAGTCCTAGGAAAAGGTCCA+AGG + chr5.3:64517943-64517962 MS.gene29480:CDS 45.0%
TATTGAATTGCAGGCGGAGA+AGG + chr5.3:64517428-64517447 MS.gene29480:intron 45.0%
TGATGGTTGCGAATGGCATA+TGG + chr5.3:64516274-64516293 MS.gene29480:CDS 45.0%
TGTTTGGCATGAATGGGTGA+CGG - chr5.3:64517492-64517511 None:intergenic 45.0%
!! ATGAATGGGTGACGGAAGTA+TGG - chr5.3:64517484-64517503 None:intergenic 45.0%
!!! TTGTAGTTTTTGGTTCCGCG+TGG + chr5.3:64518117-64518136 MS.gene29480:CDS 45.0%
AAGGTCATGGGTTCAAGTCC+TGG + chr5.3:64517060-64517079 MS.gene29480:intron 50.0%
ACTAAAGCTCCCGCATACTC+TGG - chr5.3:64517159-64517178 None:intergenic 50.0%
AGAGAGAGAGAGAGAGAGAG+AGG - chr5.3:64517527-64517546 None:intergenic 50.0%
CACCGCATATCGACAGTCAA+AGG - chr5.3:64516525-64516544 None:intergenic 50.0%
CTAAAGCTCCCGCATACTCT+GGG - chr5.3:64517158-64517177 None:intergenic 50.0%
CTGTTCCTGATGGTTGCGAA+TGG + chr5.3:64516267-64516286 MS.gene29480:CDS 50.0%
GCCACGATTACTGTTCCTGA+TGG + chr5.3:64516257-64516276 MS.gene29480:CDS 50.0%
GCTACAGTTACTGTTCCGGA+TGG + chr5.3:64517695-64517714 MS.gene29480:CDS 50.0%
TATTCTGCGGCGAATTCAGC+AGG - chr5.3:64516646-64516665 None:intergenic 50.0%
TTGACTGTCGATATGCGGTG+AGG + chr5.3:64516525-64516544 MS.gene29480:intron 50.0%
! TCGACCTTGGACCTTTTCCT+AGG - chr5.3:64517950-64517969 None:intergenic 50.0%
GCAGGCGGAGAAGGAAGTTA+GGG + chr5.3:64517437-64517456 MS.gene29480:intron 55.0%
TATGTGGCGTGACGTGTGAG+CGG - chr5.3:64517338-64517357 None:intergenic 55.0%
TGCAGGCGGAGAAGGAAGTT+AGG + chr5.3:64517436-64517455 MS.gene29480:intron 55.0%
! AAAATGGTGGGACCCCTTCC+CGG + chr5.3:64517131-64517150 MS.gene29480:intron 55.0%
! ATGCGGGAGCTTTAGTGCAC+CGG + chr5.3:64517163-64517182 MS.gene29480:intron 55.0%
! GGAAGGGGTCCCACCATTTT+TGG - chr5.3:64517131-64517150 None:intergenic 55.0%
CATACTCTGGGTCCGGGAAG+GGG - chr5.3:64517146-64517165 None:intergenic 60.0%
CTTCCCGGACCCAGAGTATG+CGG + chr5.3:64517146-64517165 MS.gene29480:intron 60.0%
GCATACTCTGGGTCCGGGAA+GGG - chr5.3:64517147-64517166 None:intergenic 60.0%
TTCCCGGACCCAGAGTATGC+GGG + chr5.3:64517147-64517166 MS.gene29480:intron 60.0%
CGCATACTCTGGGTCCGGGA+AGG - chr5.3:64517148-64517167 None:intergenic 65.0%
CTCCCGCATACTCTGGGTCC+GGG - chr5.3:64517152-64517171 None:intergenic 65.0%
GCTCCCGCATACTCTGGGTC+CGG - chr5.3:64517153-64517172 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 64516170 64518156 64516170 ID=MS.gene29480
chr5.3 mRNA 64516170 64518156 64516170 ID=MS.gene29480.t1;Parent=MS.gene29480
chr5.3 exon 64516170 64516541 64516170 ID=MS.gene29480.t1.exon1;Parent=MS.gene29480.t1
chr5.3 CDS 64516170 64516541 64516170 ID=cds.MS.gene29480.t1;Parent=MS.gene29480.t1
chr5.3 exon 64516637 64516882 64516637 ID=MS.gene29480.t1.exon2;Parent=MS.gene29480.t1
chr5.3 CDS 64516637 64516882 64516637 ID=cds.MS.gene29480.t1;Parent=MS.gene29480.t1
chr5.3 exon 64517441 64517515 64517441 ID=MS.gene29480.t1.exon3;Parent=MS.gene29480.t1
chr5.3 CDS 64517441 64517515 64517441 ID=cds.MS.gene29480.t1;Parent=MS.gene29480.t1
chr5.3 exon 64517644 64518156 64517644 ID=MS.gene29480.t1.exon4;Parent=MS.gene29480.t1
chr5.3 CDS 64517644 64518156 64517644 ID=cds.MS.gene29480.t1;Parent=MS.gene29480.t1
Gene Sequence

>MS.gene29480

ATGTATCACCCTTCCTCTAAAGATTGGTCACTACAGAGAATTCCAGATGAGTTTATAACAAGATTTGGGAATGAACTCAACAATGTTGCCACGATTACTGTTCCTGATGGTTGCGAATGGCATATGGAAGTAAAGAAACGTGGCAACGAAGTTTTCTTTTGCAAAAATTGGCAACCGTTCGCAGAACACTACTCTATCTGTTATGGTTGTTATGTAGATTTCAAGTATGAAGGGAATTCAAAGTTTAATGTTGTTATATATGATACAAATTCTGTCGAGATTTCCTATCCATTCAAAACTGGGAAGACTAATGGAGAACCAAACCCAAAAAACCCTTCTTTTCGTTCCCAATCCTTTGACTGTCGATATGCGGTGAGGATTTTACTATACATTATTTGGTTTAAAAGTAAATGTTTGAGTTATATATTTATGGTGTCACCTAAATAGATTGTTCCTCCATTGTGTAGTATGTACCTGCTGAATTCGCCGCAGAATATTTGAAGCCAAATGTTCTGTTCAAGCTCCAAAATTCTCAAGGAAAGCAGTGGGAAGTTTATTGTGTACTGAATAAAGGTGGCGGAAAAGCAATGCGAATAACGTTTGGATTCTTTAGATTTGCAAGGGAAAACAATCTATCAGATGGAGTTATGTATGAGTTTGAGCTTATCAAGAGGAAACCGCGTGTTGTGTTACAAGTTACAGCTTCTCACACGGTAAAGAGCTGTTTCACATTCGTGTATGCGTCAGTTCTATTTTCTTACAATTTTTTCTTCTAATGTTGTAACTGATGTATTGTAATTCATATAGGTAGTTTTGTTGGTTGTTAGGTTTTTTAGTTACTGATAGCTATCATCAATGACTGAAAGGTAAAGTTGTTGTCATGTGACTGAAAGGTCATGGGTTCAAGTCCTGGAAACAGCCTCTTATGTAAAAACAGGGTAAGGCTGCGTACAATACACCAAAAATGGTGGGACCCCTTCCCGGACCCAGAGTATGCGGGAGCTTTAGTGCACCGGATTGCCCTTTTTATAGCTATCATCAATGAGCAATATGGACTAAGTGTAGTTAGTAACAGAAGCAGCGAATAAAATTTGTAATCATTCTTTCTCATTATCTGATTTCAAATACTCTAAATGTGAGAGCGTGTTTTTCTTCGCGACATGAACCGCTCACACGTCACGCCACATACGAGTTCATTATTCTTTGTTTATAATGTGAATCGGTGATCTGAATGTGTGAATTGTTAACACTTATTACTTATTGAATTGCAGGCGGAGAAGGAAGTTAGGGAATCCAAACATTTTAAGAAGGCCATACTTCCGTCACCCATTCATGCCAAACAAATTGTAAATTTCACCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTATCATTATTATAATCCTATAGTAGAACCGATGAGTTATATTATCATCAATTGAACGAGTGATGATAACTGCAGAGAATTCCAGAAGACTTTATAACAATATTTGGGAATGAACTCAAGAATGTTGCTACAGTTACTGTTCCGGATGGTCTAATTTGGAAAATGGAGTTGAAGATATGTGGCAAGGACATTTTCTTTTGCAACAAATGGCAACAGTTTTCAAAATACTATTCTGTAGGATACGGTTGTTACTTGTCTTTCAAATATGAAGGGAATTCAAATTTTAGTGTTATTATATTTGATGCAACTTCTGTTGAAATTTGCTATCCATTCAAAACTCTAAGTACTAATGGAGAACCAAACAAAGAGTGTCATAGTCCTAGGAAAAGGTCCAAGGTCGAAACAAGTGAGAGTCAAGAGAAAAAGGTCAAAAACATGTCTAAATATGCTTCTAAAAGGGCTGAGGATGCTGCCAATGCATTCAGCCCAAAAAACCCTTATTTTCGTTCAAAGTGCTCCAAAGGAAGATATGTGGTGAGGATTTTACTTTGTAGTTTTTGGTTCCGCGTGGTTGACTATCGTGATTAA
Protein sequence

>MS.gene29480.t1

MYHPSSKDWSLQRIPDEFITRFGNELNNVATITVPDGCEWHMEVKKRGNEVFFCKNWQPFAEHYSICYGCYVDFKYEGNSKFNVVIYDTNSVEISYPFKTGKTNGEPNPKNPSFRSQSFDCRYAYVPAEFAAEYLKPNVLFKLQNSQGKQWEVYCVLNKGGGKAMRITFGFFRFARENNLSDGVMYEFELIKRKPRVVLQVTASHTAEKEVRESKHFKKAILPSPIHAKQIRIPEDFITIFGNELKNVATVTVPDGLIWKMELKICGKDIFFCNKWQQFSKYYSVGYGCYLSFKYEGNSNFSVIIFDATSVEICYPFKTLSTNGEPNKECHSPRKRSKVETSESQEKKVKNMSKYASKRAEDAANAFSPKNPYFRSKCSKGRYVVRILLCSFWFRVVDYRD