AlfalfaGEDB | MS.gene29855

M. sativa cultivar XinJiangDaYe / MS.gene29855

KEGG: -
GO: -
PFAM: PF03195.17

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene29855.t1	RHN68580.1	98.4	126	2	0	1	126	2	127	7.00E-64	253.1

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene29855.t1	Q9FKZ3	76.5	115	27	0	5	119	4	118	2.5e-46	186.0

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene29855.t1	A0A072UZB6	98.4	126	2	0	1	126	2	127	5.0e-64	253.1

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene29855.t1	TF	LOB

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene29855.t1	MTR_3g073690	98.413	126	2	0	1	126	2	127	3.38e-91	262
MS.gene29855.t1	MTR_5g017950	76.423	123	29	0	1	123	1	123	5.69e-67	205
MS.gene29855.t1	MTR_3g452660	74.576	118	28	2	1	117	1	117	1.97e-57	179
MS.gene29855.t1	MTR_4g105170	65.254	118	41	0	6	123	9	126	1.53e-50	159
MS.gene29855.t1	MTR_7g033800	66.667	111	37	0	2	112	5	115	7.57e-50	156
MS.gene29855.t1	MTR_3g077240	65.217	115	40	0	2	116	4	118	3.91e-49	155
MS.gene29855.t1	MTR_6g005070	67.290	107	35	0	2	108	3	109	2.13e-48	152
MS.gene29855.t1	MTR_6g005080	63.063	111	41	0	2	112	4	114	7.69e-48	150
MS.gene29855.t1	MTR_5g083230	60.684	117	45	1	6	121	4	120	5.04e-47	149
MS.gene29855.t1	MTR_3g071590	61.111	108	42	0	1	108	1	108	4.62e-46	146

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene29855.t1	AT5G66870	75.214	117	29	0	3	119	2	118	1.42e-61	191
MS.gene29855.t1	AT2G23660	66.379	116	39	0	6	121	3	118	4.52e-56	177
MS.gene29855.t1	AT2G23660	66.379	116	39	0	6	121	3	118	4.52e-56	177
MS.gene29855.t1	AT2G23660	66.379	116	39	0	6	121	3	118	4.52e-56	177
MS.gene29855.t1	AT5G63090	69.643	112	32	1	2	111	3	114	1.34e-50	159
MS.gene29855.t1	AT5G63090	69.643	112	32	1	2	111	3	114	1.34e-50	159
MS.gene29855.t1	AT5G63090	69.643	112	32	1	2	111	3	114	1.34e-50	159
MS.gene29855.t1	AT5G63090	69.643	112	32	1	2	111	3	114	1.34e-50	159
MS.gene29855.t1	AT3G27650	64.602	113	40	0	1	113	32	144	1.05e-49	155
MS.gene29855.t1	AT2G30130	62.963	108	40	0	1	108	1	108	4.81e-48	152

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Find 15 sgRNAs with CRISPR-Local

Find 20 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGAACTTGCTTCTTATGT+TGG	0.411259	3.4:-72336726	MS.gene29855:CDS
ACCAAACACTTTGTGAACGT+AGG	0.770701	3.4:+72336929	None:intergenic
ACCTACGTTCACAAAGTGTT+TGG	0.430629	3.4:-72336930	MS.gene29855:CDS
AGACCAACACAACCATAAAC+AGG	0.459225	3.4:+72336807	None:intergenic
AGTGTTTGGTGCTAGCAACG+TGG	0.743523	3.4:-72336916	MS.gene29855:CDS
CATGCTTCCTACACCGATGA+CGG	0.624741	3.4:-72336694	MS.gene29855:CDS
CGTGGCAAAGTTACTCAACG+AGG	0.719799	3.4:-72336898	MS.gene29855:CDS
CGTTTGAGAGATCCTGTTTA+TGG	0.254736	3.4:-72336819	MS.gene29855:CDS
GAACTCGACGCCGCCGTCAT+CGG	0.569433	3.4:+72336681	None:intergenic
GACGCCGCCGTCATCGGTGT+AGG	0.623332	3.4:+72336687	None:intergenic

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CRISPR-GE

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badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	ACCAAACACTTTGTGAACGT+AGG	+	chr3.4:72336800-72336819	None:intergenic	40.0%
	AGACCAACACAACCATAAAC+AGG	+	chr3.4:72336922-72336941	None:intergenic	40.0%
	CGTTTGAGAGATCCTGTTTA+TGG	-	chr3.4:72336907-72336926	MS.gene29855:CDS	40.0%
	GATCCTGTTTATGGTTGTGT+TGG	-	chr3.4:72336916-72336935	MS.gene29855:CDS	40.0%
	CATGCTTCCTACACCGATGA+CGG	-	chr3.4:72337032-72337051	MS.gene29855:CDS	50.0%
	CGTGGCAAAGTTACTCAACG+AGG	-	chr3.4:72336828-72336847	MS.gene29855:CDS	50.0%
	TGAATCTTGCACGCTGCACA+CGG	+	chr3.4:72336709-72336728	None:intergenic	50.0%
	GACGCCGCCGTCATCGGTGT+AGG	+	chr3.4:72337042-72337061	None:intergenic	70.0%
!	AAAGAACTTGCTTCTTATGT+TGG	-	chr3.4:72337000-72337019	MS.gene29855:CDS	30.0%
!	TGAACGTAGGTAAACTTTTG+AGG	+	chr3.4:72336787-72336806	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3.4	gene	72336685	72337063	72336685	ID=MS.gene29855
chr3.4	mRNA	72336685	72337063	72336685	ID=MS.gene29855.t1;Parent=MS.gene29855
chr3.4	exon	72336685	72337063	72336685	ID=MS.gene29855.t1.exon1;Parent=MS.gene29855.t1
chr3.4	CDS	72336685	72337063	72336685	ID=cds.MS.gene29855.t1;Parent=MS.gene29855.t1

Gene Sequence	>MS.gene29855 ATGTCGTCGTGTAATTCTTCTCCGTGTGCAGCGTGCAAGATTCAGCGTCGAAAATGCACAAAGGAATGCATTTTTGCACCCTATTTTCCGCCGGAAAATCCTCAAAAGTTTACCTACGTTCACAAAGTGTTTGGTGCTAGCAACGTGGCAAAGTTACTCAACGAGGTTGATGCTTCTCAGCGTGAAGATGCTGTTAAGTCTTTAGCTTATGAAGCAGAAGCGCGTTTGAGAGATCCTGTTTATGGTTGTGTTGGTCTTATTTCACATCTTCAGAATAAGCTTAAGAATTTGCAGAATGAACTTAATGTTGCTAAGAAAGAACTTGCTTCTTATGTTGGTCCTCAGATCATGCTTCCTACACCGATGACGGCGGCGTCGA
Protein sequence	>MS.gene29855.t1 MSSCNSSPCAACKIQRRKCTKECIFAPYFPPENPQKFTYVHKVFGASNVAKLLNEVDASQREDAVKSLAYEAEARLRDPVYGCVGLISHLQNKLKNLQNELNVAKKELASYVGPQIMLPTPMTAAS