Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29900.t1 | XP_024639353.1 | 89.4 | 85 | 9 | 0 | 1 | 85 | 47 | 131 | 8.90E-39 | 169.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29900.t1 | G7JX45 | 89.4 | 85 | 9 | 0 | 1 | 85 | 25 | 109 | 6.4e-39 | 169.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene29900.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29900.t1 | MTR_5g053510 | 89.412 | 85 | 9 | 0 | 1 | 85 | 25 | 109 | 3.10e-52 | 159 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 16 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATACTAAGTTAAAAAATGGT+TGG | - | 57244:14220-14239 | MS.gene29900:CDS | 20.0% |
| !! | CTGAATACTAAGTTAAAAAA+TGG | - | 57244:14216-14235 | MS.gene29900:CDS | 20.0% |
| !! | TACTAAGTTAAAAAATGGTT+GGG | - | 57244:14221-14240 | MS.gene29900:CDS | 20.0% |
| ! | AGACTTCAATGATTTCTTAT+GGG | - | 57244:14104-14123 | MS.gene29900:CDS | 25.0% |
| CAGACTTCAATGATTTCTTA+TGG | - | 57244:14103-14122 | MS.gene29900:CDS | 30.0% | |
| CATAAGAAATCATTGAAGTC+TGG | + | 57244:14105-14124 | MS.gene29900:intergenic | 30.0% | |
| TTCCTATAGACATGAACAAA+AGG | + | 57244:14321-14340 | MS.gene29900:intergenic | 30.0% | |
| AACAACAATGCATTCCAGAT+TGG | - | 57244:14255-14274 | MS.gene29900:CDS | 35.0% | |
| AAGAAATCATTGAAGTCTGG+TGG | + | 57244:14102-14121 | MS.gene29900:intergenic | 35.0% | |
| GATTTGCATTGCACAGAAAA+AGG | + | 57244:14146-14165 | MS.gene29900:intergenic | 35.0% | |
| GCTGTAACAAACTCTTCATT+CGG | + | 57244:14168-14187 | MS.gene29900:intergenic | 35.0% | |
| TATTCAGCGATTCATTGCTT+TGG | + | 57244:14203-14222 | MS.gene29900:intergenic | 35.0% | |
| ! | GTCCTTTTGTTCATGTCTAT+AGG | - | 57244:14316-14335 | MS.gene29900:CDS | 35.0% |
| !! | ATTCGTTTCAAGTTCTTCGA+TGG | - | 57244:14285-14304 | MS.gene29900:CDS | 35.0% |
| !! | TTCGTTTCAAGTTCTTCGAT+GGG | - | 57244:14286-14305 | MS.gene29900:CDS | 35.0% |
| AACGAATAGTGTCTCCAATC+TGG | + | 57244:14272-14291 | MS.gene29900:intergenic | 40.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 57244 | gene | 14093 | 14350 | 14093 | ID=MS.gene29900 |
| 57244 | mRNA | 14093 | 14350 | 14093 | ID=MS.gene29900.t1;Parent=MS.gene29900 |
| 57244 | exon | 14093 | 14350 | 14093 | ID=MS.gene29900.t1.exon1;Parent=MS.gene29900.t1 |
| 57244 | CDS | 14093 | 14350 | 14093 | ID=cds.MS.gene29900.t1;Parent=MS.gene29900.t1 |
| Gene Sequence |
| Protein sequence |
