AlfalfaGEDB | MS.gene30463

M. sativa cultivar XinJiangDaYe / MS.gene30463

KEGG: -
GO: -
PFAM: PF03101.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene30463.t1	ABD33394.2	97.9	146	3	0	1	146	443	588	3.30E-74	287.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene30463.t1	Q9SZL8	52.1	119	51	3	20	136	63	177	2.7e-24	113.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene30463.t1	Q2HRZ3	97.9	146	3	0	1	146	443	588	2.4e-74	287.7

TFs/TRs:

Gene ID	Type	Classification
MS.gene30463.t1	TF	FAR1

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene30463.t1	MTR_3g006370	60.748	107	42	0	31	137	47	153	4.22e-43	143
MS.gene30463.t1	MTR_4g067290	42.029	138	70	3	2	137	28	157	6.40e-28	104
MS.gene30463.t1	MTR_4g067290	42.029	138	70	3	2	137	28	157	6.83e-28	104
MS.gene30463.t1	MTR_4g067290	42.029	138	70	3	2	137	46	175	1.05e-27	103
MS.gene30463.t1	MTR_4g067290	42.029	138	70	3	2	137	48	177	1.14e-27	103
MS.gene30463.t1	MTR_4g067290	42.029	138	70	3	2	137	48	177	1.14e-27	103
MS.gene30463.t1	MTR_4g067290	42.029	138	70	3	2	137	88	217	1.80e-27	104
MS.gene30463.t1	MTR_2g098920	48.624	109	50	2	31	137	77	181	5.97e-27	102
MS.gene30463.t1	MTR_2g098880	39.370	127	69	3	15	138	45	166	3.56e-26	100
MS.gene30463.t1	MTR_2g098880	39.370	127	69	3	15	138	57	178	5.07e-26	99.8
MS.gene30463.t1	MTR_7g053103	67.742	62	10	2	1	62	344	395	8.30e-17	76.6
MS.gene30463.t1	MTR_2g070590	38.462	104	62	2	34	136	17	119	1.37e-16	72.0
MS.gene30463.t1	MTR_6g017200	38.144	97	58	2	34	129	58	153	8.53e-14	65.5
MS.gene30463.t1	MTR_0402s0040	37.500	104	63	2	34	136	26	128	1.24e-13	67.8
MS.gene30463.t1	MTR_4g035820	34.646	127	71	3	34	155	75	194	1.38e-12	64.7
MS.gene30463.t1	MTR_5g015480	36.190	105	63	3	34	136	56	158	2.73e-12	63.2
MS.gene30463.t1	MTR_3g450850	32.000	150	84	5	13	153	30	170	1.18e-11	62.0
MS.gene30463.t1	MTR_1g057920	34.737	95	52	3	34	123	34	123	2.11e-11	58.5
MS.gene30463.t1	MTR_1g070355	37.000	100	60	3	39	136	79	177	3.53e-11	60.8
MS.gene30463.t1	MTR_7g061980	36.538	104	64	2	33	136	9	110	6.78e-11	57.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene30463.t1	AT4G12850	60.448	134	44	2	29	157	6	135	5.99e-52	162
MS.gene30463.t1	AT4G12850	69.369	111	30	1	29	139	6	112	3.87e-51	162
MS.gene30463.t1	AT4G12850	59.559	136	44	2	29	157	6	137	4.31e-51	160
MS.gene30463.t1	AT2G43280	58.654	104	43	0	33	136	21	124	6.50e-41	136
MS.gene30463.t1	AT2G43280	59.551	89	36	0	49	137	2	90	2.61e-35	121
MS.gene30463.t1	AT3G07500	52.679	112	52	1	33	144	32	142	7.11e-33	116
MS.gene30463.t1	AT3G59470	50.877	114	52	2	25	136	59	170	2.19e-30	110
MS.gene30463.t1	AT3G59470	50.877	114	52	2	25	136	59	170	2.19e-30	110
MS.gene30463.t1	AT3G59470	50.877	114	52	2	25	136	71	182	2.82e-30	110
MS.gene30463.t1	AT4G38180	52.101	119	51	3	20	136	63	177	1.24e-27	108
MS.gene30463.t1	AT5G18960	46.154	104	42	1	33	136	42	131	1.63e-21	90.5
MS.gene30463.t1	AT5G18960	43.269	104	45	2	33	136	210	299	1.03e-18	82.8
MS.gene30463.t1	AT5G18960	46.154	104	42	1	33	136	42	131	1.63e-21	90.5
MS.gene30463.t1	AT5G18960	43.269	104	45	2	33	136	210	299	1.03e-18	82.8
MS.gene30463.t1	AT3G06250	45.192	104	43	1	33	136	27	116	7.91e-21	88.6
MS.gene30463.t1	AT3G06250	44.231	104	44	2	33	136	189	278	1.59e-19	85.1
MS.gene30463.t1	AT3G06250	45.192	104	43	1	33	136	27	116	7.91e-21	88.6
MS.gene30463.t1	AT3G06250	44.231	104	44	2	33	136	189	278	1.59e-19	85.1
MS.gene30463.t1	AT3G06250	45.192	104	43	1	33	136	27	116	7.91e-21	88.6
MS.gene30463.t1	AT3G06250	44.231	104	44	2	33	136	189	278	1.59e-19	85.1
MS.gene30463.t1	AT2G32250	34.711	121	73	2	18	136	20	136	3.13e-16	75.5
MS.gene30463.t1	AT2G32250	34.711	121	73	2	18	136	20	136	3.13e-16	75.5
MS.gene30463.t1	AT2G32250	34.711	121	73	2	18	136	20	136	3.13e-16	75.5
MS.gene30463.t1	AT2G32250	34.711	121	73	2	18	136	20	136	3.22e-16	75.5
MS.gene30463.t1	AT2G32250	34.711	121	73	2	18	136	20	136	3.41e-16	75.5
MS.gene30463.t1	AT2G32250	34.711	121	73	2	18	136	20	136	3.41e-16	75.5
MS.gene30463.t1	AT4G15090	34.783	115	73	2	33	146	50	163	1.69e-15	73.6
MS.gene30463.t1	AT4G15090	34.783	115	73	2	33	146	50	163	1.69e-15	73.6
MS.gene30463.t1	AT4G15090	34.783	115	73	2	33	146	50	163	1.69e-15	73.6
MS.gene30463.t1	AT4G15090	34.783	115	73	2	33	146	50	163	1.69e-15	73.6
MS.gene30463.t1	AT4G15090	34.783	115	73	2	33	146	50	163	1.69e-15	73.6
MS.gene30463.t1	AT2G32250	36.364	99	59	1	38	136	1	95	3.41e-15	72.4
MS.gene30463.t1	AT2G32250	36.364	99	59	1	38	136	1	95	3.41e-15	72.4
MS.gene30463.t1	AT4G12850	66.667	42	14	0	29	70	6	47	5.48e-15	65.9
MS.gene30463.t1	AT2G27110	32.500	120	65	2	19	138	34	137	8.47e-15	71.2
MS.gene30463.t1	AT2G27110	32.500	120	65	2	19	138	34	137	8.47e-15	71.2
MS.gene30463.t1	AT1G76320	36.364	99	59	1	38	136	1	95	9.39e-14	68.2
MS.gene30463.t1	AT1G76320	36.364	99	59	1	38	136	1	95	9.62e-14	68.2
MS.gene30463.t1	AT1G76320	36.364	99	59	1	38	136	1	95	1.04e-13	68.2
MS.gene30463.t1	AT1G76320	36.364	99	59	1	38	136	1	95	1.04e-13	68.2
MS.gene30463.t1	AT3G22170	32.759	116	66	2	33	136	69	184	9.94e-13	65.5
MS.gene30463.t1	AT3G22170	32.759	116	66	2	33	136	69	184	9.94e-13	65.5
MS.gene30463.t1	AT4G19990	32.168	143	75	5	13	136	10	149	1.06e-12	65.5
MS.gene30463.t1	AT4G19990	33.600	125	68	4	24	136	14	135	2.48e-12	64.3
MS.gene30463.t1	AT4G19990	33.600	125	68	4	24	136	14	135	2.48e-12	64.3
MS.gene30463.t1	AT4G19990	33.600	125	68	4	24	136	14	135	2.48e-12	64.3
MS.gene30463.t1	AT4G19990	33.600	125	68	4	24	136	14	135	2.67e-12	64.3
MS.gene30463.t1	AT4G19990	33.600	125	68	4	24	136	14	135	2.67e-12	64.3
MS.gene30463.t1	AT1G52520	37.398	123	65	4	18	136	68	182	4.54e-12	63.5

Find 38 sgRNAs with CRISPR-Local

Find 40 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCCAATGTTCCCTTGCTATT+TGG	0.143180	6.4:+40429810	None:intergenic
CCTCTAACTGCCATAGTTAA+TGG	0.164800	6.4:-40429672	MS.gene30463:CDS
CTGCCATAGTTAATGGTTTC+AGG	0.184902	6.4:-40429665	MS.gene30463:CDS
AGGTTGCATGGCAACAATTT+TGG	0.264590	6.4:-40429754	MS.gene30463:CDS
AGAAGAGAGCACACATATAT+TGG	0.339545	6.4:-40430018	MS.gene30463:CDS
CCTTCTCGTGCACTAGGCCT+TGG	0.392893	6.4:+40429774	None:intergenic
ATATTCAGCATAAAACTTCC+TGG	0.428965	6.4:+40429938	None:intergenic
CGTCCTGAAACCATTAACTA+TGG	0.429169	6.4:+40429662	None:intergenic
TCTTATGGATTCTGTGGTAG+TGG	0.430837	6.4:-40430078	None:intergenic
CCATTAACTATGGCAGTTAG+AGG	0.431747	6.4:+40429672	None:intergenic
ACCAAATAGCAAGGGAACAT+TGG	0.440465	6.4:-40429811	MS.gene30463:CDS
CCAAATAGCAAGGGAACATT+GGG	0.442775	6.4:-40429810	MS.gene30463:CDS
TCAAACTCCATGCCCACATA+TGG	0.450379	6.4:+40429975	None:intergenic
CGTAGACGATCTGGAATTGA+TGG	0.457844	6.4:-40429879	MS.gene30463:CDS
AGGACTCTTGCTCGTCGACT+TGG	0.484621	6.4:-40429855	MS.gene30463:CDS
ATGCAACCTTCTCGTGCACT+AGG	0.514269	6.4:+40429768	None:intergenic
TTGATTCTGAAGAAGCTGCC+AGG	0.517746	6.4:-40429956	MS.gene30463:CDS
TTATGCTGAATATGCTAGAC+GGG	0.518296	6.4:-40429928	MS.gene30463:CDS
GTTATGCAACGTAGACGATC+TGG	0.528818	6.4:-40429888	MS.gene30463:CDS
CGAAGATCTACTTAAACAAG+TGG	0.535198	6.4:+40429591	None:intergenic
GGATTCAGACAAGGGAAATG+AGG	0.564058	6.4:-40430057	MS.gene30463:CDS
GACGATCTGGAATTGATGGC+AGG	0.564371	6.4:-40429875	MS.gene30463:CDS
GCTGAATATGCTAGACGGGT+AGG	0.564964	6.4:-40429924	MS.gene30463:CDS
TCAAGAGCCATATGTGGGCA+TGG	0.577246	6.4:-40429982	MS.gene30463:CDS
TAACTATGGCAGTTAGAGGA+TGG	0.578331	6.4:+40429676	None:intergenic
TGAACTTCAAGAGCCATATG+TGG	0.585856	6.4:-40429988	MS.gene30463:CDS
AGGATTCTCACCAAATAGCA+AGG	0.586863	6.4:-40429820	MS.gene30463:CDS
CGACTTGGATGTAACAAACA+AGG	0.598498	6.4:-40429840	MS.gene30463:CDS
GCAGATTCTTATGGATTCTG+TGG	0.610998	6.4:-40430084	None:intergenic
TTTATGCTGAATATGCTAGA+CGG	0.611450	6.4:-40429929	MS.gene30463:CDS
AGTTAATGGTTTCAGGACGG+AGG	0.630209	6.4:-40429658	MS.gene30463:CDS
CCAAGGCCTAGTGCACGAGA+AGG	0.635511	6.4:-40429774	MS.gene30463:CDS
CATAGTTAATGGTTTCAGGA+CGG	0.641364	6.4:-40429661	MS.gene30463:CDS
GAACTTCAAGAGCCATATGT+GGG	0.645554	6.4:-40429987	MS.gene30463:CDS
TAGTGCACGAGAAGGTTGCA+TGG	0.650503	6.4:-40429766	MS.gene30463:CDS
GGATTCTCACCAAATAGCAA+GGG	0.654395	6.4:-40429819	MS.gene30463:CDS
TGTGGTAGTGGATTCAGACA+AGG	0.656451	6.4:-40430066	MS.gene30463:CDS
GTGGTAGTGGATTCAGACAA+GGG	0.661730	6.4:-40430065	MS.gene30463:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AGATGGAAAAATCTGGAAAA+TGG	-	chr6.4:40429948-40429967	MS.gene30463:CDS	30.0%
	ATATTCAGCATAAAACTTCC+TGG	+	chr6.4:40429741-40429760	None:intergenic	30.0%
	GATGGAAAAATCTGGAAAAT+GGG	-	chr6.4:40429949-40429968	MS.gene30463:CDS	30.0%
	TTGGTTAAGATGGAAAAATC+TGG	-	chr6.4:40429941-40429960	MS.gene30463:CDS	30.0%
	TTTATGCTGAATATGCTAGA+CGG	-	chr6.4:40429747-40429766	MS.gene30463:CDS	30.0%
	CATAGTTAATGGTTTCAGGA+CGG	-	chr6.4:40430015-40430034	MS.gene30463:CDS	35.0%
	TGGGAAAAGAAAAAAGACCA+AGG	-	chr6.4:40429885-40429904	MS.gene30463:CDS	35.0%
	TTATGCTGAATATGCTAGAC+GGG	-	chr6.4:40429748-40429767	MS.gene30463:CDS	35.0%
!	AGAAGAGAGCACACATATAT+TGG	-	chr6.4:40429658-40429677	MS.gene30463:CDS	35.0%
!!!	GGCAACAATTTTGGTTAAGA+TGG	-	chr6.4:40429931-40429950	MS.gene30463:CDS	35.0%
	ACCAAATAGCAAGGGAACAT+TGG	-	chr6.4:40429865-40429884	MS.gene30463:CDS	40.0%
	AGGATTCTCACCAAATAGCA+AGG	-	chr6.4:40429856-40429875	MS.gene30463:CDS	40.0%
	CCAAATAGCAAGGGAACATT+GGG	-	chr6.4:40429866-40429885	MS.gene30463:CDS	40.0%
	CCATTAACTATGGCAGTTAG+AGG	+	chr6.4:40430007-40430026	None:intergenic	40.0%
	CCTCTAACTGCCATAGTTAA+TGG	-	chr6.4:40430004-40430023	MS.gene30463:CDS	40.0%
	CGTCCTGAAACCATTAACTA+TGG	+	chr6.4:40430017-40430036	None:intergenic	40.0%
	CTGCCATAGTTAATGGTTTC+AGG	-	chr6.4:40430011-40430030	MS.gene30463:CDS	40.0%
	GAACTTCAAGAGCCATATGT+GGG	-	chr6.4:40429689-40429708	MS.gene30463:CDS	40.0%
	GGATTCTCACCAAATAGCAA+GGG	-	chr6.4:40429857-40429876	MS.gene30463:CDS	40.0%
	TAACTATGGCAGTTAGAGGA+TGG	+	chr6.4:40430003-40430022	None:intergenic	40.0%
	TGAACTTCAAGAGCCATATG+TGG	-	chr6.4:40429688-40429707	MS.gene30463:CDS	40.0%
!	AGGTTGCATGGCAACAATTT+TGG	-	chr6.4:40429922-40429941	MS.gene30463:CDS	40.0%
!	CGACTTGGATGTAACAAACA+AGG	-	chr6.4:40429836-40429855	MS.gene30463:CDS	40.0%
	AGTTAATGGTTTCAGGACGG+AGG	-	chr6.4:40430018-40430037	MS.gene30463:CDS	45.0%
	GGATTCAGACAAGGGAAATG+AGG	-	chr6.4:40429619-40429638	MS.gene30463:CDS	45.0%
	GTTATGCAACGTAGACGATC+TGG	-	chr6.4:40429788-40429807	MS.gene30463:CDS	45.0%
	TCAAACTCCATGCCCACATA+TGG	+	chr6.4:40429704-40429723	None:intergenic	45.0%
!	CGTAGACGATCTGGAATTGA+TGG	-	chr6.4:40429797-40429816	MS.gene30463:CDS	45.0%
!	TTGATTCTGAAGAAGCTGCC+AGG	-	chr6.4:40429720-40429739	MS.gene30463:CDS	45.0%
!!	CCCAATGTTCCCTTGCTATT+TGG	+	chr6.4:40429869-40429888	None:intergenic	45.0%
!!	GTGGTAGTGGATTCAGACAA+GGG	-	chr6.4:40429611-40429630	MS.gene30463:CDS	45.0%
!!	TGTGGTAGTGGATTCAGACA+AGG	-	chr6.4:40429610-40429629	MS.gene30463:CDS	45.0%
	ATGCAACCTTCTCGTGCACT+AGG	+	chr6.4:40429911-40429930	None:intergenic	50.0%
	GCTGAATATGCTAGACGGGT+AGG	-	chr6.4:40429752-40429771	MS.gene30463:CDS	50.0%
	TAGTGCACGAGAAGGTTGCA+TGG	-	chr6.4:40429910-40429929	MS.gene30463:CDS	50.0%
	TCAAGAGCCATATGTGGGCA+TGG	-	chr6.4:40429694-40429713	MS.gene30463:CDS	50.0%
!	GACGATCTGGAATTGATGGC+AGG	-	chr6.4:40429801-40429820	MS.gene30463:CDS	50.0%
!	AGGACTCTTGCTCGTCGACT+TGG	-	chr6.4:40429821-40429840	MS.gene30463:CDS	55.0%
	CCAAGGCCTAGTGCACGAGA+AGG	-	chr6.4:40429902-40429921	MS.gene30463:CDS	60.0%
	CCTTCTCGTGCACTAGGCCT+TGG	+	chr6.4:40429905-40429924	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.4	gene	40429602	40430096	40429602	ID=MS.gene30463
chr6.4	mRNA	40429602	40430096	40429602	ID=MS.gene30463.t1;Parent=MS.gene30463
chr6.4	exon	40429602	40430096	40429602	ID=MS.gene30463.t1.exon1;Parent=MS.gene30463.t1
chr6.4	CDS	40429602	40430096	40429602	ID=cds.MS.gene30463.t1;Parent=MS.gene30463.t1

Gene Sequence

>MS.gene30463

ATGGATTCTGTGGTAGTGGATTCAGACAAGGGAAATGAGGCAGATCATAGCTTTCTAGAAGAGAGCACACATATATTGGATAGAGTTGAACTTCAAGAGCCATATGTGGGCATGGAGTTTGATTCTGAAGAAGCTGCCAGGAAGTTTTATGCTGAATATGCTAGACGGGTAGGATTTGTTGTACGCGTTATGCAACGTAGACGATCTGGAATTGATGGCAGGACTCTTGCTCGTCGACTTGGATGTAACAAACAAGGATTCTCACCAAATAGCAAGGGAACATTGGGAAAAGAAAAAAGACCAAGGCCTAGTGCACGAGAAGGTTGCATGGCAACAATTTTGGTTAAGATGGAAAAATCTGGAAAATGGGTTGTCACAAGATTTATAAAAGATCACAACCATCCTCTAACTGCCATAGTTAATGGTTTCAGGACGGAGGTTAGTTTTCTGCTCTCTTATAATGTTTGTTTTTACTATAGCTTTCCACTTGTTTAA

Protein sequence

>MS.gene30463.t1

MDSVVVDSDKGNEADHSFLEESTHILDRVELQEPYVGMEFDSEEAARKFYAEYARRVGFVVRVMQRRRSGIDGRTLARRLGCNKQGFSPNSKGTLGKEKRPRPSAREGCMATILVKMEKSGKWVVTRFIKDHNHPLTAIVNGFRTEVSFLLSYNVCFYYSFPLV