AlfalfaGEDB | MS.gene30464

M. sativa cultivar XinJiangDaYe / MS.gene30464

KEGG: -
GO: -
PFAM: PF03101.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene30464.t1	ABD33394.2	98.6	146	2	0	1	146	443	588	2.90E-75	291.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene30464.t1	Q9SZL8	52.1	119	51	3	20	136	63	177	2.6e-24	113.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene30464.t1	Q2HRZ3	98.6	146	2	0	1	146	443	588	2.1e-75	291.2

TFs/TRs:

Gene ID	Type	Classification
MS.gene30464.t1	TF	FAR1

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene30464.t1	MTR_3g006370	60.185	108	43	0	31	138	47	154	2.27e-43	143
MS.gene30464.t1	MTR_4g067290	42.754	138	69	3	2	137	28	157	3.00e-29	107
MS.gene30464.t1	MTR_4g067290	42.754	138	69	3	2	137	28	157	3.16e-29	107
MS.gene30464.t1	MTR_4g067290	42.754	138	69	3	2	137	46	175	5.06e-29	107
MS.gene30464.t1	MTR_4g067290	42.754	138	69	3	2	137	48	177	5.76e-29	107
MS.gene30464.t1	MTR_4g067290	42.754	138	69	3	2	137	48	177	5.82e-29	107
MS.gene30464.t1	MTR_4g067290	42.754	138	69	3	2	137	88	217	9.53e-29	107
MS.gene30464.t1	MTR_2g098920	46.512	129	59	4	31	157	77	197	9.59e-28	104
MS.gene30464.t1	MTR_2g098880	39.370	127	69	3	15	138	45	166	2.00e-26	100
MS.gene30464.t1	MTR_2g098880	39.370	127	69	3	15	138	57	178	2.59e-26	100
MS.gene30464.t1	MTR_7g053103	67.742	62	10	2	1	62	344	395	4.61e-17	77.4
MS.gene30464.t1	MTR_2g070590	38.462	104	62	2	34	136	17	119	6.65e-17	72.4
MS.gene30464.t1	MTR_6g017200	38.144	97	58	2	34	129	58	153	4.45e-14	65.9
MS.gene30464.t1	MTR_0402s0040	37.500	104	63	2	34	136	26	128	5.05e-14	68.9
MS.gene30464.t1	MTR_5g015480	36.190	105	63	3	34	136	56	158	1.47e-12	63.9
MS.gene30464.t1	MTR_3g450850	33.083	133	76	4	13	136	30	158	3.65e-12	63.2
MS.gene30464.t1	MTR_1g057920	34.737	95	52	3	34	123	34	123	1.17e-11	58.9
MS.gene30464.t1	MTR_1g070355	36.634	101	61	3	38	136	78	177	1.97e-11	61.2
MS.gene30464.t1	MTR_7g061980	36.538	104	64	2	33	136	9	110	4.71e-11	57.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene30464.t1	AT4G12850	69.369	111	30	1	29	139	6	112	1.96e-51	161
MS.gene30464.t1	AT4G12850	69.369	111	30	1	29	139	6	112	2.54e-51	162
MS.gene30464.t1	AT4G12850	69.369	111	30	1	29	139	6	112	3.60e-51	160
MS.gene30464.t1	AT2G43280	58.095	105	44	0	33	137	21	125	3.38e-41	137
MS.gene30464.t1	AT2G43280	58.889	90	37	0	49	138	2	91	1.15e-35	122
MS.gene30464.t1	AT3G07500	52.679	112	52	1	33	144	32	142	2.25e-33	117
MS.gene30464.t1	AT3G59470	50.877	114	52	2	25	136	59	170	1.38e-30	111
MS.gene30464.t1	AT3G59470	50.877	114	52	2	25	136	59	170	1.38e-30	111
MS.gene30464.t1	AT3G59470	50.877	114	52	2	25	136	71	182	1.95e-30	111
MS.gene30464.t1	AT4G38180	51.667	120	52	3	20	137	63	178	1.12e-27	108
MS.gene30464.t1	AT5G18960	46.154	104	42	1	33	136	42	131	1.29e-21	90.9
MS.gene30464.t1	AT5G18960	43.269	104	45	2	33	136	210	299	1.11e-18	82.4
MS.gene30464.t1	AT5G18960	46.154	104	42	1	33	136	42	131	1.29e-21	90.9
MS.gene30464.t1	AT5G18960	43.269	104	45	2	33	136	210	299	1.11e-18	82.4
MS.gene30464.t1	AT3G06250	45.192	104	43	1	33	136	27	116	6.44e-21	89.0
MS.gene30464.t1	AT3G06250	44.231	104	44	2	33	136	189	278	1.51e-19	84.7
MS.gene30464.t1	AT3G06250	45.192	104	43	1	33	136	27	116	6.44e-21	89.0
MS.gene30464.t1	AT3G06250	44.231	104	44	2	33	136	189	278	1.51e-19	84.7
MS.gene30464.t1	AT3G06250	45.192	104	43	1	33	136	27	116	6.44e-21	89.0
MS.gene30464.t1	AT3G06250	44.231	104	44	2	33	136	189	278	1.51e-19	84.7
MS.gene30464.t1	AT2G32250	34.426	122	74	2	18	137	20	137	1.53e-16	76.3
MS.gene30464.t1	AT2G32250	34.426	122	74	2	18	137	20	137	1.69e-16	76.3
MS.gene30464.t1	AT2G32250	34.426	122	74	2	18	137	20	137	1.69e-16	76.3
MS.gene30464.t1	AT2G32250	34.426	122	74	2	18	137	20	137	1.69e-16	76.3
MS.gene30464.t1	AT2G32250	34.426	122	74	2	18	137	20	137	1.73e-16	76.3
MS.gene30464.t1	AT2G32250	34.426	122	74	2	18	137	20	137	1.73e-16	76.3
MS.gene30464.t1	AT4G15090	34.783	115	73	2	33	146	50	163	1.10e-15	73.9
MS.gene30464.t1	AT4G15090	34.783	115	73	2	33	146	50	163	1.10e-15	73.9
MS.gene30464.t1	AT4G15090	34.783	115	73	2	33	146	50	163	1.10e-15	73.9
MS.gene30464.t1	AT4G15090	34.783	115	73	2	33	146	50	163	1.10e-15	73.9
MS.gene30464.t1	AT4G15090	34.783	115	73	2	33	146	50	163	1.10e-15	73.9
MS.gene30464.t1	AT2G32250	36.000	100	60	1	38	137	1	96	2.22e-15	72.8
MS.gene30464.t1	AT2G32250	36.000	100	60	1	38	137	1	96	2.31e-15	72.8
MS.gene30464.t1	AT4G12850	66.667	42	14	0	29	70	6	47	4.04e-15	65.9
MS.gene30464.t1	AT2G27110	32.500	120	65	2	19	138	34	137	1.39e-14	70.5
MS.gene30464.t1	AT2G27110	32.500	120	65	2	19	138	34	137	1.39e-14	70.5
MS.gene30464.t1	AT1G76320	36.364	99	59	1	38	136	1	95	8.33e-14	68.2
MS.gene30464.t1	AT1G76320	36.364	99	59	1	38	136	1	95	8.33e-14	68.2
MS.gene30464.t1	AT1G76320	36.364	99	59	1	38	136	1	95	8.57e-14	68.2
MS.gene30464.t1	AT1G76320	36.364	99	59	1	38	136	1	95	8.78e-14	68.2
MS.gene30464.t1	AT3G22170	32.031	128	74	3	21	136	58	184	7.61e-13	65.5
MS.gene30464.t1	AT3G22170	32.031	128	74	3	21	136	58	184	7.61e-13	65.5
MS.gene30464.t1	AT4G19990	32.168	143	75	5	13	136	10	149	8.85e-13	65.5
MS.gene30464.t1	AT4G19990	33.600	125	68	4	24	136	14	135	1.50e-12	64.7
MS.gene30464.t1	AT4G19990	33.600	125	68	4	24	136	14	135	1.50e-12	64.7
MS.gene30464.t1	AT4G19990	33.600	125	68	4	24	136	14	135	1.56e-12	64.7
MS.gene30464.t1	AT4G19990	33.600	125	68	4	24	136	14	135	1.56e-12	64.7
MS.gene30464.t1	AT4G19990	33.600	125	68	4	24	136	14	135	1.70e-12	64.7
MS.gene30464.t1	AT1G52520	36.290	124	65	4	18	136	68	182	3.17e-11	60.8

Find 42 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCCAATGTTCCCTTGCTATT+TGG	0.143180	6.4:+40436348	None:intergenic
CCTCTAACTGCCATAGTTAA+TGG	0.164800	6.4:-40436210	MS.gene30464:CDS
CTGCCATAGTTAATGGTTTC+AGG	0.184902	6.4:-40436203	MS.gene30464:CDS
AGGTTGCATGGCAACAATTT+TGG	0.264590	6.4:-40436292	MS.gene30464:CDS
AGAAGAGAGCACACATATAT+TGG	0.339545	6.4:-40436556	MS.gene30464:CDS
CCTTCTCGTGCACTAGGCCT+TGG	0.392893	6.4:+40436312	None:intergenic
GGTTTCAGGACAGAGGTGTT+AGG	0.408762	6.4:-40436189	MS.gene30464:CDS
ATATTCAGCATAAAACTTCC+TGG	0.428965	6.4:+40436476	None:intergenic
TCTTATGGATTCTGTGGTAG+TGG	0.430837	6.4:-40436616	None:intergenic
CCATTAACTATGGCAGTTAG+AGG	0.431747	6.4:+40436210	None:intergenic
TGTCCTGAAACCATTAACTA+TGG	0.437750	6.4:+40436200	None:intergenic
ACCAAATAGCAAGGGAACAT+TGG	0.440465	6.4:-40436349	MS.gene30464:CDS
CCAAATAGCAAGGGAACATT+GGG	0.442775	6.4:-40436348	MS.gene30464:CDS
TCAAACTCCATGCCCACATA+TGG	0.450379	6.4:+40436513	None:intergenic
CGTAGACGATCTGGAATTGA+TGG	0.457844	6.4:-40436417	MS.gene30464:CDS
AGGACTCTTGCTCGTCGACT+TGG	0.484621	6.4:-40436393	MS.gene30464:CDS
ATGCAACCTTCTCGTGCACT+AGG	0.514269	6.4:+40436306	None:intergenic
TTGATTCTGAAGAAGCTGCC+AGG	0.517746	6.4:-40436494	MS.gene30464:CDS
TTATGCTGAATATGCTAGAC+GGG	0.518296	6.4:-40436466	MS.gene30464:CDS
GTTATGCAACGTAGACGATC+TGG	0.528818	6.4:-40436426	MS.gene30464:CDS
GGATTCAGACAAGGGAAATG+AGG	0.564058	6.4:-40436595	MS.gene30464:CDS
GACGATCTGGAATTGATGGC+AGG	0.564371	6.4:-40436413	MS.gene30464:CDS
GCTGAATATGCTAGACGGGT+AGG	0.564964	6.4:-40436462	MS.gene30464:CDS
GAGAGCACACATATATTGGA+TGG	0.565400	6.4:-40436552	MS.gene30464:CDS
TCAAGAGCCATATGTGGGCA+TGG	0.577246	6.4:-40436520	MS.gene30464:CDS
TAACTATGGCAGTTAGAGGA+TGG	0.578331	6.4:+40436214	None:intergenic
TGAACTTCAAGAGCCATATG+TGG	0.585856	6.4:-40436526	MS.gene30464:CDS
AGGATTCTCACCAAATAGCA+AGG	0.586863	6.4:-40436358	MS.gene30464:CDS
ACTTCTACTTAATCTGACCA+TGG	0.588097	6.4:+40436148	None:intergenic
CTTCTACTTAATCTGACCAT+GGG	0.590711	6.4:+40436149	None:intergenic
CGACTTGGATGTAACAAACA+AGG	0.598498	6.4:-40436378	MS.gene30464:CDS
GCAGATTCTTATGGATTCTG+TGG	0.610998	6.4:-40436622	None:intergenic
TTTATGCTGAATATGCTAGA+CGG	0.611450	6.4:-40436467	MS.gene30464:CDS
CCAAGGCCTAGTGCACGAGA+AGG	0.635511	6.4:-40436312	MS.gene30464:CDS
AACTATGCACAATTTCCCCA+TGG	0.640407	6.4:-40436165	MS.gene30464:CDS
AGTTAATGGTTTCAGGACAG+AGG	0.640431	6.4:-40436196	MS.gene30464:CDS
GAACTTCAAGAGCCATATGT+GGG	0.645554	6.4:-40436525	MS.gene30464:CDS
TAGTGCACGAGAAGGTTGCA+TGG	0.650503	6.4:-40436304	MS.gene30464:CDS
GGATTCTCACCAAATAGCAA+GGG	0.654395	6.4:-40436357	MS.gene30464:CDS
TGTGGTAGTGGATTCAGACA+AGG	0.656451	6.4:-40436604	MS.gene30464:CDS
GTGGTAGTGGATTCAGACAA+GGG	0.661730	6.4:-40436603	MS.gene30464:CDS
TTCTACTTAATCTGACCATG+GGG	0.695566	6.4:+40436150	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AGATGGAAAAATCTGGAAAA+TGG	-	chr6.4:40436498-40436517	MS.gene30464:CDS	30.0%
	ATATTCAGCATAAAACTTCC+TGG	+	chr6.4:40436291-40436310	None:intergenic	30.0%
	GATGGAAAAATCTGGAAAAT+GGG	-	chr6.4:40436499-40436518	MS.gene30464:CDS	30.0%
	TTGGTTAAGATGGAAAAATC+TGG	-	chr6.4:40436491-40436510	MS.gene30464:CDS	30.0%
	TTTATGCTGAATATGCTAGA+CGG	-	chr6.4:40436297-40436316	MS.gene30464:CDS	30.0%
	TGGGAAAAGAAAAAAGACCA+AGG	-	chr6.4:40436435-40436454	MS.gene30464:CDS	35.0%
	TGTCCTGAAACCATTAACTA+TGG	+	chr6.4:40436567-40436586	None:intergenic	35.0%
	TTATGCTGAATATGCTAGAC+GGG	-	chr6.4:40436298-40436317	MS.gene30464:CDS	35.0%
!	AGAAGAGAGCACACATATAT+TGG	-	chr6.4:40436208-40436227	MS.gene30464:CDS	35.0%
!!!	GGCAACAATTTTGGTTAAGA+TGG	-	chr6.4:40436481-40436500	MS.gene30464:CDS	35.0%
	AACTATGCACAATTTCCCCA+TGG	-	chr6.4:40436599-40436618	MS.gene30464:CDS	40.0%
	ACCAAATAGCAAGGGAACAT+TGG	-	chr6.4:40436415-40436434	MS.gene30464:CDS	40.0%
	AGGATTCTCACCAAATAGCA+AGG	-	chr6.4:40436406-40436425	MS.gene30464:CDS	40.0%
	AGTTAATGGTTTCAGGACAG+AGG	-	chr6.4:40436568-40436587	MS.gene30464:CDS	40.0%
	CCAAATAGCAAGGGAACATT+GGG	-	chr6.4:40436416-40436435	MS.gene30464:CDS	40.0%
	CCATTAACTATGGCAGTTAG+AGG	+	chr6.4:40436557-40436576	None:intergenic	40.0%
	CCTCTAACTGCCATAGTTAA+TGG	-	chr6.4:40436554-40436573	MS.gene30464:CDS	40.0%
	CTGCCATAGTTAATGGTTTC+AGG	-	chr6.4:40436561-40436580	MS.gene30464:CDS	40.0%
	GAACTTCAAGAGCCATATGT+GGG	-	chr6.4:40436239-40436258	MS.gene30464:CDS	40.0%
	GGATTCTCACCAAATAGCAA+GGG	-	chr6.4:40436407-40436426	MS.gene30464:CDS	40.0%
	TAACTATGGCAGTTAGAGGA+TGG	+	chr6.4:40436553-40436572	None:intergenic	40.0%
	TGAACTTCAAGAGCCATATG+TGG	-	chr6.4:40436238-40436257	MS.gene30464:CDS	40.0%
!	AGGTTGCATGGCAACAATTT+TGG	-	chr6.4:40436472-40436491	MS.gene30464:CDS	40.0%
!	CGACTTGGATGTAACAAACA+AGG	-	chr6.4:40436386-40436405	MS.gene30464:CDS	40.0%
!	GAGAGCACACATATATTGGA+TGG	-	chr6.4:40436212-40436231	MS.gene30464:CDS	40.0%
	GGATTCAGACAAGGGAAATG+AGG	-	chr6.4:40436169-40436188	MS.gene30464:CDS	45.0%
	GTTATGCAACGTAGACGATC+TGG	-	chr6.4:40436338-40436357	MS.gene30464:CDS	45.0%
	TCAAACTCCATGCCCACATA+TGG	+	chr6.4:40436254-40436273	None:intergenic	45.0%
!	CGTAGACGATCTGGAATTGA+TGG	-	chr6.4:40436347-40436366	MS.gene30464:CDS	45.0%
!	TTGATTCTGAAGAAGCTGCC+AGG	-	chr6.4:40436270-40436289	MS.gene30464:CDS	45.0%
!!	CCCAATGTTCCCTTGCTATT+TGG	+	chr6.4:40436419-40436438	None:intergenic	45.0%
!!	GTGGTAGTGGATTCAGACAA+GGG	-	chr6.4:40436161-40436180	MS.gene30464:CDS	45.0%
!!	TGTGGTAGTGGATTCAGACA+AGG	-	chr6.4:40436160-40436179	MS.gene30464:CDS	45.0%
	ATGCAACCTTCTCGTGCACT+AGG	+	chr6.4:40436461-40436480	None:intergenic	50.0%
	GCTGAATATGCTAGACGGGT+AGG	-	chr6.4:40436302-40436321	MS.gene30464:CDS	50.0%
	TAGTGCACGAGAAGGTTGCA+TGG	-	chr6.4:40436460-40436479	MS.gene30464:CDS	50.0%
	TCAAGAGCCATATGTGGGCA+TGG	-	chr6.4:40436244-40436263	MS.gene30464:CDS	50.0%
!	GACGATCTGGAATTGATGGC+AGG	-	chr6.4:40436351-40436370	MS.gene30464:CDS	50.0%
!!	GGTTTCAGGACAGAGGTGTT+AGG	-	chr6.4:40436575-40436594	MS.gene30464:CDS	50.0%
!	AGGACTCTTGCTCGTCGACT+TGG	-	chr6.4:40436371-40436390	MS.gene30464:CDS	55.0%
	CCAAGGCCTAGTGCACGAGA+AGG	-	chr6.4:40436452-40436471	MS.gene30464:CDS	60.0%
	CCTTCTCGTGCACTAGGCCT+TGG	+	chr6.4:40436455-40436474	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.4	gene	40436152	40436634	40436152	ID=MS.gene30464
chr6.4	mRNA	40436152	40436634	40436152	ID=MS.gene30464.t1;Parent=MS.gene30464
chr6.4	exon	40436152	40436634	40436152	ID=MS.gene30464.t1.exon1;Parent=MS.gene30464.t1
chr6.4	CDS	40436152	40436634	40436152	ID=cds.MS.gene30464.t1;Parent=MS.gene30464.t1

Gene Sequence

>MS.gene30464

ATGGATTCTGTGGTAGTGGATTCAGACAAGGGAAATGAGGCAGATCATAGCTTTCTAGAAGAGAGCACACATATATTGGATGGAGTTGAACTTCAAGAGCCATATGTGGGCATGGAGTTTGATTCTGAAGAAGCTGCCAGGAAGTTTTATGCTGAATATGCTAGACGGGTAGGATTTGTTGTACGCGTTATGCAACGTAGACGATCTGGAATTGATGGCAGGACTCTTGCTCGTCGACTTGGATGTAACAAACAAGGATTCTCACCAAATAGCAAGGGAACATTGGGAAAAGAAAAAAGACCAAGGCCTAGTGCACGAGAAGGTTGCATGGCAACAATTTTGGTTAAGATGGAAAAATCTGGAAAATGGGTTGTCACAAGATTTATAAAAGATCACAACCATCCTCTAACTGCCATAGTTAATGGTTTCAGGACAGAGGTGTTAGGAAACTATGCACAATTTCCCCATGGTCAGATTAAGTAG

Protein sequence

>MS.gene30464.t1

MDSVVVDSDKGNEADHSFLEESTHILDGVELQEPYVGMEFDSEEAARKFYAEYARRVGFVVRVMQRRRSGIDGRTLARRLGCNKQGFSPNSKGTLGKEKRPRPSAREGCMATILVKMEKSGKWVVTRFIKDHNHPLTAIVNGFRTEVLGNYAQFPHGQIK