AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene31080

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31080.t1 MTR_1g008290 33.333 141 83 3 68 201 8 144 4.03e-21 90.5
MS.gene31080.t1 MTR_3g006370 32.335 167 99 4 9 168 45 204 4.23e-19 87.4
MS.gene31080.t1 MTR_0402s0040 35.338 133 77 5 7 133 21 150 7.15e-15 78.2
MS.gene31080.t1 MTR_2g098880 35.644 101 60 1 18 113 65 165 8.80e-14 72.0
MS.gene31080.t1 MTR_2g098880 35.644 101 60 1 18 113 77 177 1.07e-13 72.0
MS.gene31080.t1 MTR_5g032260 41.975 81 41 2 49 124 302 381 3.60e-13 72.4
MS.gene31080.t1 MTR_6g022070 24.878 205 139 3 224 418 4 203 9.39e-13 70.5
MS.gene31080.t1 MTR_6g051425 24.336 226 149 6 21 228 24 245 1.15e-12 70.1
MS.gene31080.t1 MTR_2g070590 33.636 110 67 3 7 112 12 119 2.17e-11 62.0
MS.gene31080.t1 MTR_5g015480 27.358 212 137 6 14 214 56 261 2.27e-11 65.1
MS.gene31080.t1 MTR_4g067290 33.036 112 69 3 10 117 52 161 2.87e-11 64.3
MS.gene31080.t1 MTR_4g067290 33.036 112 69 3 10 117 112 221 2.89e-11 65.5
MS.gene31080.t1 MTR_4g067290 33.036 112 69 3 10 117 52 161 2.98e-11 64.3
MS.gene31080.t1 MTR_4g067290 33.036 112 69 3 10 117 72 181 3.16e-11 64.7
MS.gene31080.t1 MTR_4g067290 33.036 112 69 3 10 117 70 179 3.17e-11 64.7
MS.gene31080.t1 MTR_4g067290 33.036 112 69 3 10 117 72 181 3.19e-11 64.7
MS.gene31080.t1 MTR_0856s0010 31.034 116 78 2 13 127 33 147 3.22e-11 62.4
MS.gene31080.t1 MTR_2g098920 33.036 112 69 3 10 117 76 185 6.64e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31080.t1 AT4G15090 42.834 628 260 10 13 546 50 672 1.18e-167 501
MS.gene31080.t1 AT4G15090 42.834 628 260 10 13 546 50 672 1.18e-167 501
MS.gene31080.t1 AT4G15090 42.834 628 260 10 13 546 50 672 1.18e-167 501
MS.gene31080.t1 AT4G15090 42.834 628 260 10 13 546 50 672 1.18e-167 501
MS.gene31080.t1 AT4G15090 42.834 628 260 10 13 546 50 672 1.18e-167 501
MS.gene31080.t1 AT1G76320 53.483 445 195 7 228 663 290 731 1.91e-158 474
MS.gene31080.t1 AT1G76320 70.755 212 61 1 18 228 1 212 1.14e-99 321
MS.gene31080.t1 AT1G76320 53.483 445 195 7 228 663 290 731 1.91e-158 474
MS.gene31080.t1 AT1G76320 70.755 212 61 1 18 228 1 212 1.14e-99 321
MS.gene31080.t1 AT1G76320 53.604 444 194 7 228 663 290 729 5.11e-158 473
MS.gene31080.t1 AT1G76320 70.755 212 61 1 18 228 1 212 1.21e-99 321
MS.gene31080.t1 AT1G76320 57.218 381 156 3 228 602 290 669 7.17e-152 455
MS.gene31080.t1 AT1G76320 70.755 212 61 1 18 228 1 212 1.93e-100 322
MS.gene31080.t1 AT3G22170 49.689 322 160 2 223 543 357 677 9.34e-108 345
MS.gene31080.t1 AT3G22170 43.103 232 100 3 13 228 69 284 2.23e-50 188
MS.gene31080.t1 AT3G22170 49.689 322 160 2 223 543 357 677 9.34e-108 345
MS.gene31080.t1 AT3G22170 43.103 232 100 3 13 228 69 284 2.23e-50 188
MS.gene31080.t1 AT4G19990 48.012 327 166 3 224 546 240 566 9.45e-91 296
MS.gene31080.t1 AT4G19990 41.146 192 66 5 54 228 4 165 4.95e-29 123
MS.gene31080.t1 AT4G19990 48.012 327 166 3 224 546 297 623 2.03e-90 297
MS.gene31080.t1 AT4G19990 44.053 227 80 5 19 228 26 222 7.85e-42 162
MS.gene31080.t1 AT4G19990 48.012 327 166 3 224 546 297 623 2.03e-90 297
MS.gene31080.t1 AT4G19990 44.053 227 80 5 19 228 26 222 7.85e-42 162
MS.gene31080.t1 AT4G19990 48.012 327 166 3 224 546 297 623 2.18e-90 297
MS.gene31080.t1 AT4G19990 44.053 227 80 5 19 228 26 222 7.38e-42 162
MS.gene31080.t1 AT4G19990 48.012 327 166 3 224 546 297 623 2.38e-90 298
MS.gene31080.t1 AT4G19990 44.053 227 80 5 19 228 26 222 1.31e-41 162
MS.gene31080.t1 AT4G19990 48.012 327 166 3 224 546 297 623 2.38e-90 298
MS.gene31080.t1 AT4G19990 44.053 227 80 5 19 228 26 222 1.31e-41 162
MS.gene31080.t1 AT4G19990 48.012 327 166 3 224 546 311 637 2.78e-90 297
MS.gene31080.t1 AT4G19990 44.053 227 80 5 19 228 40 236 6.69e-42 163
MS.gene31080.t1 AT2G32250 42.188 320 184 1 225 543 253 572 7.01e-83 277
MS.gene31080.t1 AT2G32250 44.550 211 84 3 18 228 1 178 1.62e-42 164
MS.gene31080.t1 AT2G32250 42.188 320 184 1 225 543 294 613 9.00e-83 277
MS.gene31080.t1 AT2G32250 42.667 225 96 3 4 228 28 219 4.89e-44 169
MS.gene31080.t1 AT2G32250 42.188 320 184 1 225 543 253 572 9.63e-83 277
MS.gene31080.t1 AT2G32250 44.550 211 84 3 18 228 1 178 1.84e-42 164
MS.gene31080.t1 AT2G32250 42.188 320 184 1 225 543 294 613 1.40e-82 278
MS.gene31080.t1 AT2G32250 42.667 225 96 3 4 228 28 219 7.47e-44 169
MS.gene31080.t1 AT2G32250 42.188 320 184 1 225 543 294 613 1.40e-82 278
MS.gene31080.t1 AT2G32250 42.667 225 96 3 4 228 28 219 7.47e-44 169
MS.gene31080.t1 AT2G32250 42.188 320 184 1 225 543 294 613 1.40e-82 278
MS.gene31080.t1 AT2G32250 42.667 225 96 3 4 228 28 219 7.47e-44 169
MS.gene31080.t1 AT2G32250 42.188 320 184 1 225 543 294 613 1.51e-82 277
MS.gene31080.t1 AT2G32250 42.667 225 96 3 4 228 28 219 8.34e-44 169
MS.gene31080.t1 AT2G32250 42.188 320 184 1 225 543 294 613 1.51e-82 277
MS.gene31080.t1 AT2G32250 42.667 225 96 3 4 228 28 219 8.34e-44 169
MS.gene31080.t1 AT4G38180 36.842 323 197 3 223 538 372 694 3.71e-66 232
MS.gene31080.t1 AT4G38180 38.865 229 116 7 13 223 72 294 4.04e-30 127
MS.gene31080.t1 AT2G27110 35.258 329 207 4 224 548 175 501 2.52e-60 215
MS.gene31080.t1 AT2G27110 35.258 329 207 4 224 548 320 646 8.87e-60 215
MS.gene31080.t1 AT2G27110 31.111 225 124 8 1 217 34 235 7.17e-16 82.0
MS.gene31080.t1 AT2G27110 35.258 329 207 4 224 548 320 646 8.87e-60 215
MS.gene31080.t1 AT2G27110 31.111 225 124 8 1 217 34 235 7.17e-16 82.0
MS.gene31080.t1 AT1G52520 31.437 334 201 6 223 536 369 694 6.33e-49 182
MS.gene31080.t1 AT1G52520 30.837 227 132 7 14 231 85 295 4.60e-17 85.9
MS.gene31080.t1 AT4G38170 31.832 333 218 4 224 549 138 468 4.77e-47 175
MS.gene31080.t1 AT3G06250 36.466 266 151 5 224 482 449 703 1.09e-41 162
MS.gene31080.t1 AT3G06250 26.214 206 121 4 13 217 189 364 8.19e-14 75.5
MS.gene31080.t1 AT3G06250 36.466 266 151 5 224 482 449 703 1.09e-41 162
MS.gene31080.t1 AT3G06250 26.214 206 121 4 13 217 189 364 8.19e-14 75.5
MS.gene31080.t1 AT3G06250 36.466 266 151 5 224 482 449 703 1.09e-41 162
MS.gene31080.t1 AT3G06250 26.214 206 121 4 13 217 189 364 8.19e-14 75.5
MS.gene31080.t1 AT1G80010 30.909 330 207 8 223 539 394 715 3.86e-39 154
MS.gene31080.t1 AT1G80010 29.018 224 133 4 14 218 95 311 6.16e-16 82.4
MS.gene31080.t1 AT5G18960 33.333 261 166 4 224 482 473 727 2.44e-37 149
MS.gene31080.t1 AT5G18960 28.037 214 122 5 8 218 205 389 1.31e-14 78.2
MS.gene31080.t1 AT5G18960 33.333 261 166 4 224 482 473 727 2.44e-37 149
MS.gene31080.t1 AT5G18960 28.037 214 122 5 8 218 205 389 1.31e-14 78.2
MS.gene31080.t1 AT1G10240 26.207 290 196 5 224 505 351 630 1.47e-30 128
MS.gene31080.t1 AT1G10240 26.207 290 196 5 224 505 351 630 1.47e-30 128
MS.gene31080.t1 AT5G28530 24.665 523 318 16 9 478 50 549 1.27e-26 115
MS.gene31080.t1 AT5G28530 24.478 527 318 16 9 478 50 553 1.38e-26 115
MS.gene31080.t1 AT5G28530 27.757 263 176 6 218 478 362 612 4.89e-20 95.1
MS.gene31080.t1 AT5G28530 27.757 263 176 6 218 478 362 612 4.89e-20 95.1
MS.gene31080.t1 AT5G28530 27.341 267 176 6 218 478 362 616 5.56e-20 95.1
MS.gene31080.t1 AT2G43280 31.544 149 96 2 13 155 21 169 1.73e-17 82.0
MS.gene31080.t1 AT3G59470 38.835 103 57 3 19 117 75 175 1.00e-14 75.1
MS.gene31080.t1 AT3G59470 38.835 103 57 3 19 117 75 175 1.00e-14 75.1
MS.gene31080.t1 AT3G59470 38.835 103 57 3 19 117 87 187 1.22e-14 75.1
MS.gene31080.t1 AT2G43280 30.827 133 86 2 29 155 2 134 2.11e-14 72.0
MS.gene31080.t1 AT3G07500 37.288 118 71 1 8 122 27 144 2.48e-14 73.2
MS.gene31080.t1 AT4G12850 35.294 102 66 0 11 112 8 109 2.11e-13 68.6
MS.gene31080.t1 AT4G12850 35.294 102 66 0 11 112 8 109 2.63e-13 68.2
MS.gene31080.t1 AT4G12850 35.294 102 66 0 11 112 8 109 1.08e-12 67.4

Find 127 sgRNAs with CRISPR-Local

Find 283 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAAATTCCATTTCATTATT+AGG 0.082825 2.4:-5747628 None:intergenic
TATGGATTCTTCTATTATTA+TGG 0.187730 2.4:+5747588 None:intergenic
TCTAAAGGGTTGGAGGATTT+TGG 0.197839 2.4:+5748234 MS.gene31080:CDS
CTTCAACATTGCAACCAATA+TGG 0.212680 2.4:-5749475 None:intergenic
ACCTTCTTCACCCAATATTA+TGG 0.238949 2.4:-5749348 None:intergenic
AAAGTTTCAGTTCGAGGTTT+TGG 0.254700 2.4:+5749001 MS.gene31080:CDS
CAAATTCCATTTCATTATTA+GGG 0.256855 2.4:-5747627 None:intergenic
GAAAGTTACTACATGGCTTT+AGG 0.264793 2.4:+5749383 MS.gene31080:CDS
TGTCACTTGTTTGAATATAA+AGG 0.267118 2.4:+5749152 MS.gene31080:CDS
TACATGGCATGATAGCTTTA+TGG 0.278309 2.4:+5748581 MS.gene31080:CDS
GTTTATGAAGGATATTTCTT+TGG 0.305630 2.4:+5748746 MS.gene31080:CDS
ATGAATCGTCAACAAGATAA+AGG 0.325098 2.4:+5748072 MS.gene31080:CDS
GATGGTGGAAGCTGGTTGAT+AGG 0.330003 2.4:+5748658 MS.gene31080:CDS
AGTTAAGTAGTCGCCGATCT+AGG 0.332224 2.4:+5747716 MS.gene31080:CDS
TCAATGGCATAAAAGAATTT+CGG 0.332652 2.4:-5748168 None:intergenic
CTTAAGAACTCAAGCTGTTT+CGG 0.341887 2.4:-5748558 None:intergenic
AAGCCATGTAGTAACTTTCT+TGG 0.341888 2.4:-5749379 None:intergenic
GGGGAAGGTGACTTTAATTC+AGG 0.347918 2.4:-5748933 None:intergenic
CCAAGCATCAAAATTTGCTT+TGG 0.356859 2.4:-5748901 None:intergenic
GGTGTAGCTGGTAGGAGTTC+AGG 0.372841 2.4:+5749578 MS.gene31080:CDS
AGGAGGTGTTGAAGGAAGTA+AGG 0.375618 2.4:+5749598 MS.gene31080:CDS
ACAAGATAAAGGGCGGAGTT+TGG 0.376984 2.4:+5748083 MS.gene31080:CDS
TGTAGACGAGCCATAATATT+GGG 0.380324 2.4:+5749338 MS.gene31080:CDS
GCAGCCTTAGCTACCCATAT+TGG 0.380779 2.4:+5749461 MS.gene31080:CDS
ATCGGCTTAGAAATGTGTTC+TGG 0.382613 2.4:+5748205 MS.gene31080:CDS
TTCAACATTGCAACCAATAT+GGG 0.390404 2.4:-5749474 None:intergenic
GTATCAGAATATTGATTGTT+TGG 0.395189 2.4:+5748041 MS.gene31080:CDS
AGGAGTTCAGGAGGTGTTGA+AGG 0.401164 2.4:+5749590 MS.gene31080:CDS
TTCAAACAAGTGACAGGAAC+AGG 0.401839 2.4:-5749144 None:intergenic
ATGTAGACGAGCCATAATAT+TGG 0.410423 2.4:+5749337 MS.gene31080:CDS
CCGCGTCCTTCCCCGAAAAT+CGG 0.414368 2.4:+5747817 MS.gene31080:CDS
CTGGGTTGATTCTAAAGGGT+TGG 0.419557 2.4:+5748224 MS.gene31080:CDS
TTAAACTTAGAGATGTTAAG+TGG 0.419663 2.4:+5748682 MS.gene31080:CDS
AGAGAAAACAGGATGGTAAA+TGG 0.421573 2.4:+5747863 MS.gene31080:CDS
TTGCTTTAGAGCCTCGCTTA+AGG 0.423117 2.4:-5749408 None:intergenic
ATGAAGGATATTTCTTTGGC+TGG 0.424174 2.4:+5748750 MS.gene31080:CDS
TCAAGACCCTAATAATGAAA+TGG 0.426644 2.4:+5747621 MS.gene31080:CDS
AGCCATGTAGTAACTTTCTT+GGG 0.427360 2.4:-5749378 None:intergenic
CTATCAAAAGTTTCAGTTCG+AGG 0.433567 2.4:+5748995 MS.gene31080:CDS
TCTTTGGCTGGCTTGTCTAC+CGG 0.435556 2.4:+5748762 MS.gene31080:CDS
CTCTATCTTCAAGAATCAAT+TGG 0.436835 2.4:-5748866 None:intergenic
ATCATTTAATCTCCGGACCT+TGG 0.446449 2.4:-5749312 None:intergenic
GTGTTCTGGGTTGATTCTAA+AGG 0.446481 2.4:+5748219 MS.gene31080:CDS
TGTTCTGGGTTGATTCTAAA+GGG 0.454484 2.4:+5748220 MS.gene31080:CDS
AAAGAGTATGCAAAATCAGC+AGG 0.457157 2.4:+5747679 MS.gene31080:CDS
TATGCAAAATCAGCAGGGTT+TGG 0.458192 2.4:+5747685 MS.gene31080:CDS
TAACCATGGCTTTCAACAAA+TGG 0.462363 2.4:+5749738 MS.gene31080:CDS
TCAGAGACCTTATCGTTAGA+TGG 0.469626 2.4:-5749518 None:intergenic
GACAGGAGCATACTGATATA+AGG 0.470455 2.4:+5751541 MS.gene31080:intron
GGTTGATTCTAAAGGGTTGG+AGG 0.473041 2.4:+5748227 MS.gene31080:CDS
TGAATTGTTTATGCATATGC+AGG 0.475105 2.4:+5748137 MS.gene31080:CDS
ATTGTTGTTCTACAAATGTC+TGG 0.476184 2.4:+5749194 MS.gene31080:CDS
GTATCCTATTGGTGAAAACT+TGG 0.480416 2.4:+5749277 MS.gene31080:CDS
GTCATATAGGTGCCTCAGCT+TGG 0.482653 2.4:+5749700 MS.gene31080:intron
AGTAAGAAGGCTGCAAGGAC+TGG 0.483811 2.4:+5749557 MS.gene31080:CDS
ATATGGTAATAAACAACAAT+CGG 0.483815 2.4:+5747780 MS.gene31080:CDS
ATGCTCTTCATTCAAATCAA+TGG 0.484513 2.4:-5748184 None:intergenic
TACATAAATCATTTAATCTC+CGG 0.485589 2.4:-5749319 None:intergenic
GCTGCAAGGACTGGTGTAGC+TGG 0.488741 2.4:+5749566 MS.gene31080:CDS
TCTTGAAGATAGAGATGAAG+AGG 0.488747 2.4:+5748875 MS.gene31080:CDS
TGAAAAGAGATGGTGGAAGC+TGG 0.490757 2.4:+5748650 MS.gene31080:CDS
TATTATACCATCTAACGATA+AGG 0.493893 2.4:+5749511 MS.gene31080:CDS
TTGGGTGCCAACGTTTATGA+AGG 0.495738 2.4:+5748734 MS.gene31080:CDS
CGGAGGAAGAATTCGACTGC+AGG 0.496134 2.4:+5747997 MS.gene31080:CDS
ATCAGAACTACATGGTAGAA+TGG 0.499877 2.4:+5749099 MS.gene31080:CDS
CAAAGTATATATTGAAAAGA+TGG 0.500276 2.4:+5749234 MS.gene31080:CDS
ACAAATGTATATATCAATCA+TGG 0.501166 2.4:+5748613 MS.gene31080:CDS
TGAATCGTCAACAAGATAAA+GGG 0.505965 2.4:+5748073 MS.gene31080:CDS
TCGGCTTAGAAATGTGTTCT+GGG 0.507619 2.4:+5748206 MS.gene31080:CDS
GCTTTAAGTAGGTATCCTAT+TGG 0.508050 2.4:+5749266 MS.gene31080:CDS
GTATGATGATCGTGCATGTT+GGG 0.511716 2.4:+5748716 MS.gene31080:CDS
GCCATAATATTGGGTGAAGA+AGG 0.513780 2.4:+5749347 MS.gene31080:CDS
TATGCATGTGAAGAGAAAAC+AGG 0.515366 2.4:+5747852 MS.gene31080:CDS
TTTATATTCAAACAAGTGAC+AGG 0.515815 2.4:-5749150 None:intergenic
AGCAATTTGAAAAGAGATGG+TGG 0.516518 2.4:+5748643 MS.gene31080:CDS
AGCTTGAGTTCTTAAGTACA+TGG 0.520700 2.4:+5748565 MS.gene31080:CDS
ATCCCAAGAAAGTTACTACA+TGG 0.521925 2.4:+5749376 MS.gene31080:CDS
TAAACTTTCAAAGCGCGAGC+CGG 0.522584 2.4:-5748781 None:intergenic
GCAGTTTCACAGCATGGTGC+CGG 0.523705 2.4:+5751584 MS.gene31080:CDS
ATACCATTTGTTGAAAGCCA+TGG 0.528664 2.4:-5749741 None:intergenic
ATTCTTTCGATGCCCTAGAT+CGG 0.531419 2.4:-5747729 None:intergenic
AAGAGGTTCAATCCAAGGTC+CGG 0.533736 2.4:+5749300 MS.gene31080:CDS
CAACAATTGCATGTCTACAT+AGG 0.534107 2.4:-5749178 None:intergenic
TATTGGTGAAAACTTGGAAG+AGG 0.534284 2.4:+5749283 MS.gene31080:CDS
GTTAAGTAGTCGCCGATCTA+GGG 0.536955 2.4:+5747717 MS.gene31080:CDS
TTGATTTGAATGAAGAGCAT+CGG 0.543981 2.4:+5748187 MS.gene31080:CDS
GAAATATCCTTCATAAACGT+TGG 0.547589 2.4:-5748741 None:intergenic
GTCTTAATGAAGTATCAACT+TGG 0.551353 2.4:-5748827 None:intergenic
CGAAAATAATCAGAACTACA+TGG 0.553895 2.4:+5749091 MS.gene31080:CDS
AGAAGCAATTGAGCATTCCC+CGG 0.554547 2.4:-5748114 None:intergenic
GAAAATGAATACGAGTAAGA+AGG 0.555256 2.4:+5749544 MS.gene31080:CDS
GGCTTTAGGTTCCTTAAGCG+AGG 0.555372 2.4:+5749397 MS.gene31080:CDS
CATCTAAAGAAAGAAAATGA+TGG 0.556147 2.4:+5749038 MS.gene31080:CDS
GCAACCAATATGGGTAGCTA+AGG 0.558177 2.4:-5749465 None:intergenic
GGAGGTGTTGAAGGAAGTAA+GGG 0.558225 2.4:+5749599 MS.gene31080:CDS
AGTTGAGGCTGCACTGTGAA+AGG 0.558850 2.4:-5751634 None:intergenic
ACCATGCTGTGAAACTGCAC+TGG 0.564697 2.4:-5751579 None:intergenic
CAAGGACTGGTGTAGCTGGT+AGG 0.565226 2.4:+5749570 MS.gene31080:CDS
ATACGTTGAGTAATGATGTT+CGG 0.569177 2.4:+5747968 MS.gene31080:CDS
GCCAGTGCAGTTTCACAGCA+TGG 0.570308 2.4:+5751578 MS.gene31080:CDS
CCTCAGCTTGGAGCAGTAAG+TGG 0.574296 2.4:+5749712 MS.gene31080:CDS
GTTGAGGCTGCACTGTGAAA+GGG 0.574401 2.4:-5751633 None:intergenic
AAGAATTCGACTGCAGGTGG+TGG 0.578130 2.4:+5748003 MS.gene31080:CDS
TGTATGATGATCGTGCATGT+TGG 0.580054 2.4:+5748715 MS.gene31080:CDS
AGGAGCAATTTGAAAAGAGA+TGG 0.582141 2.4:+5748640 MS.gene31080:CDS
GGACAAACGTCGCTTTAAGT+AGG 0.584827 2.4:+5749255 MS.gene31080:CDS
TAAACTTAGAGATGTTAAGT+GGG 0.586727 2.4:+5748683 MS.gene31080:CDS
CTTGGAAGAGGTTCAATCCA+AGG 0.589927 2.4:+5749295 MS.gene31080:CDS
GTTTCACAGCATGGTGCCGG+CGG 0.596454 2.4:+5751587 MS.gene31080:CDS
TGTTGAAGGAAGTAAGGGAA+AGG 0.609259 2.4:+5749604 MS.gene31080:CDS
GTAATGATGTTCGGATGAGA+CGG 0.611128 2.4:+5747977 MS.gene31080:CDS
GCAGTAAGTGGTAGTAACCA+TGG 0.616206 2.4:+5749724 MS.gene31080:CDS
AGGAAGAATTCGACTGCAGG+TGG 0.619877 2.4:+5748000 MS.gene31080:CDS
CCACTTACTGCTCCAAGCTG+AGG 0.628390 2.4:-5749712 None:intergenic
AAGAGTATGCAAAATCAGCA+GGG 0.632144 2.4:+5747680 MS.gene31080:CDS
AGAATGGAACACATCAAACT+CGG 0.636732 2.4:+5749115 MS.gene31080:CDS
GTAGCTGGTAGGAGTTCAGG+AGG 0.638776 2.4:+5749581 MS.gene31080:CDS
TTGAGGCTGCACTGTGAAAG+GGG 0.645901 2.4:-5751632 None:intergenic
ACATGCACGATCATCATACA+AGG 0.650096 2.4:-5748712 None:intergenic
TCATTATGGAACATAGCCGC+CGG 0.651164 2.4:-5751603 None:intergenic
TGAGGCTGCACTGTGAAAGG+GGG 0.670500 2.4:-5751631 None:intergenic
ATCGTCAACAAGATAAAGGG+CGG 0.671459 2.4:+5748076 MS.gene31080:CDS
TATATATCAATCATGGACAG+AGG 0.673001 2.4:+5748620 MS.gene31080:CDS
ATACGAGTAAGAAGGCTGCA+AGG 0.675579 2.4:+5749552 MS.gene31080:CDS
ATGATGTTCGGATGAGACGG+AGG 0.681860 2.4:+5747980 MS.gene31080:CDS
CATGTGAAGAGAAAACAGGA+TGG 0.699872 2.4:+5747856 MS.gene31080:CDS
ATTGTCATGCAAATGAGTTG+AGG 0.717124 2.4:-5751649 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATATTTTTTTGTATTTCAA+CGG + chr2.4:5750407-5750426 MS.gene31080:intron 10.0%
!!! AAATTTTCATGTATTAGATA+TGG + chr2.4:5747763-5747782 MS.gene31080:CDS 15.0%
!!! GTTTCATTTTAAATTGTTTA+TGG - chr2.4:5750618-5750637 None:intergenic 15.0%
!!! TGCATATTTTAAAACTAAAA+GGG + chr2.4:5750498-5750517 MS.gene31080:intron 15.0%
!!! TTTTGTCATATTTTTTTTTG+AGG + chr2.4:5750374-5750393 MS.gene31080:intron 15.0%
!! AAACTTAAAAACTACAAAAC+TGG - chr2.4:5750068-5750087 None:intergenic 20.0%
!! AATTAAAACGCTTAGATATT+TGG + chr2.4:5749956-5749975 MS.gene31080:intron 20.0%
!! ACAAATAAACCAATGAAAAT+TGG - chr2.4:5750098-5750117 None:intergenic 20.0%
!! ACAAATGTATATATCAATCA+TGG + chr2.4:5748613-5748632 MS.gene31080:CDS 20.0%
!! ACACAGAGATATTAAAAATT+TGG - chr2.4:5751515-5751534 None:intergenic 20.0%
!! ATATGGTAATAAACAACAAT+CGG + chr2.4:5747780-5747799 MS.gene31080:CDS 20.0%
!! CAAAGTATATATTGAAAAGA+TGG + chr2.4:5749234-5749253 MS.gene31080:CDS 20.0%
!! CAAATTCCATTTCATTATTA+GGG - chr2.4:5747630-5747649 None:intergenic 20.0%
!! GAAGTTATTGTTTAAATCTT+TGG + chr2.4:5751010-5751029 MS.gene31080:intron 20.0%
!! GATTTAAACAATAACTTCAA+TGG - chr2.4:5751008-5751027 None:intergenic 20.0%
!! TACATAAATCATTTAATCTC+CGG - chr2.4:5749322-5749341 None:intergenic 20.0%
!! TCAAATTCCATTTCATTATT+AGG - chr2.4:5747631-5747650 None:intergenic 20.0%
!! TTAGAATGTACTGTTATAAT+TGG - chr2.4:5751120-5751139 None:intergenic 20.0%
!! TTATTTGTCTCAAAGTTAAT+TGG + chr2.4:5750110-5750129 MS.gene31080:intron 20.0%
!!! AATTTTAAAATAGTACAAGG+AGG - chr2.4:5749657-5749676 None:intergenic 20.0%
!!! CATCTTTTCAATATATACTT+TGG - chr2.4:5749236-5749255 None:intergenic 20.0%
!!! GACAATTTTAAAATAGTACA+AGG - chr2.4:5749660-5749679 None:intergenic 20.0%
!!! GCATATTTTAAAACTAAAAG+GGG + chr2.4:5750499-5750518 MS.gene31080:intron 20.0%
!!! GTATGTTTATAGTTTTGTTA+AGG + chr2.4:5747885-5747904 MS.gene31080:CDS 20.0%
!!! GTGCATATTTTAAAACTAAA+AGG + chr2.4:5750497-5750516 MS.gene31080:intron 20.0%
!!! TAGAAATAAAGCATTTTTGT+TGG - chr2.4:5750830-5750849 None:intergenic 20.0%
!!! TATCTTTGAAAACAGTTTTT+CGG + chr2.4:5750169-5750188 MS.gene31080:intron 20.0%
!!! TTATTTTGTCCAATTTTCAT+TGG + chr2.4:5750086-5750105 MS.gene31080:intron 20.0%
!!! TTTTAATGGTTATTTCAAAG+CGG - chr2.4:5750729-5750748 None:intergenic 20.0%
! AAGTGTAAAAAAAGTTTGGA+AGG + chr2.4:5750286-5750305 MS.gene31080:intron 25.0%
! AGCCTAAACTACTATATATT+GGG - chr2.4:5751373-5751392 None:intergenic 25.0%
! AGTGTAAAAAAAGTTTGGAA+GGG + chr2.4:5750287-5750306 MS.gene31080:intron 25.0%
! ATAATGCTTAATATGTTGAC+TGG + chr2.4:5749986-5750005 MS.gene31080:intron 25.0%
! CATCTAAAGAAAGAAAATGA+TGG + chr2.4:5749038-5749057 MS.gene31080:CDS 25.0%
! GTTTATGAAGGATATTTCTT+TGG + chr2.4:5748746-5748765 MS.gene31080:CDS 25.0%
! TAAACTTAGAGATGTTAAGT+GGG + chr2.4:5748683-5748702 MS.gene31080:CDS 25.0%
! TAGCCTAAACTACTATATAT+TGG - chr2.4:5751374-5751393 None:intergenic 25.0%
! TATGTATTTGATGAGAACTT+AGG + chr2.4:5750469-5750488 MS.gene31080:intron 25.0%
! TATTATACCATCTAACGATA+AGG + chr2.4:5749511-5749530 MS.gene31080:CDS 25.0%
! TCAATGGCATAAAAGAATTT+CGG - chr2.4:5748171-5748190 None:intergenic 25.0%
! TCTTAACAGATATTTGTCAT+TGG - chr2.4:5751293-5751312 None:intergenic 25.0%
! TGTCAAGTGTAAAAAAAGTT+TGG + chr2.4:5750282-5750301 MS.gene31080:intron 25.0%
! TTAAACTTAGAGATGTTAAG+TGG + chr2.4:5748682-5748701 MS.gene31080:CDS 25.0%
! TTTATATTCAAACAAGTGAC+AGG - chr2.4:5749153-5749172 None:intergenic 25.0%
!! CATCATTTTCTTTCTTTAGA+TGG - chr2.4:5749040-5749059 None:intergenic 25.0%
!! CTTTTCAATATATACTTTGG+CGG - chr2.4:5749233-5749252 None:intergenic 25.0%
!! GTATCAGAATATTGATTGTT+TGG + chr2.4:5748041-5748060 MS.gene31080:CDS 25.0%
!! TGTCACTTGTTTGAATATAA+AGG + chr2.4:5749152-5749171 MS.gene31080:CDS 25.0%
!! TTAGGCTAGACATTAATTTT+TGG + chr2.4:5751386-5751405 MS.gene31080:intron 25.0%
!! TTCTTACTCGTATTCATTTT+CGG - chr2.4:5749545-5749564 None:intergenic 25.0%
!! TTTTCAATATATACTTTGGC+GGG - chr2.4:5749232-5749251 None:intergenic 25.0%
!!! ACATTGTCTAAAAGTACTTA+TGG + chr2.4:5750770-5750789 MS.gene31080:intron 25.0%
!!! CATTGTCTAAAAGTACTTAT+GGG + chr2.4:5750771-5750790 MS.gene31080:intron 25.0%
!!! TTTTAAAATTGTCTCTGACT+TGG + chr2.4:5749667-5749686 MS.gene31080:intron 25.0%
AAGGGAAAATAAAGAAATGC+AGG + chr2.4:5751330-5751349 MS.gene31080:intron 30.0%
AATTACATTGACATCCTATG+AGG - chr2.4:5750546-5750565 None:intergenic 30.0%
ATACGTTGAGTAATGATGTT+CGG + chr2.4:5747968-5747987 MS.gene31080:CDS 30.0%
ATCTGTTAAGATGAGACTTT+GGG + chr2.4:5751302-5751321 MS.gene31080:intron 30.0%
ATGAAACAAAAGCAAAACCA+AGG + chr2.4:5750631-5750650 MS.gene31080:intron 30.0%
ATGAATCGTCAACAAGATAA+AGG + chr2.4:5748072-5748091 MS.gene31080:CDS 30.0%
ATGCTCTTCATTCAAATCAA+TGG - chr2.4:5748187-5748206 None:intergenic 30.0%
CGAAAATAATCAGAACTACA+TGG + chr2.4:5749091-5749110 MS.gene31080:CDS 30.0%
CTCTATCTTCAAGAATCAAT+TGG - chr2.4:5748869-5748888 None:intergenic 30.0%
GAAAATGAATACGAGTAAGA+AGG + chr2.4:5749544-5749563 MS.gene31080:CDS 30.0%
GAAATATCCTTCATAAACGT+TGG - chr2.4:5748744-5748763 None:intergenic 30.0%
GAACAAATCAATTAGTAAGC+AGG + chr2.4:5751048-5751067 MS.gene31080:intron 30.0%
GACCCAATATATAGTAGTTT+AGG + chr2.4:5751368-5751387 MS.gene31080:intron 30.0%
GTGTAAAAAAAGTTTGGAAG+GGG + chr2.4:5750288-5750307 MS.gene31080:intron 30.0%
TAAAACGCTTAGATATTTGG+AGG + chr2.4:5749959-5749978 MS.gene31080:intron 30.0%
TAACAGATATTTGTCATTGG+TGG - chr2.4:5751290-5751309 None:intergenic 30.0%
TATATATCAATCATGGACAG+AGG + chr2.4:5748620-5748639 MS.gene31080:CDS 30.0%
TATCTGTTAAGATGAGACTT+TGG + chr2.4:5751301-5751320 MS.gene31080:intron 30.0%
TATTTCAACGGAGAAACAAA+CGG + chr2.4:5750419-5750438 MS.gene31080:intron 30.0%
TCAAGACCCTAATAATGAAA+TGG + chr2.4:5747621-5747640 MS.gene31080:CDS 30.0%
TCCAAAATCTAAACCAGTAA+GGG - chr2.4:5749934-5749953 None:intergenic 30.0%
TGAATCGTCAACAAGATAAA+GGG + chr2.4:5748073-5748092 MS.gene31080:CDS 30.0%
TGAATTGTTTATGCATATGC+AGG + chr2.4:5748137-5748156 MS.gene31080:CDS 30.0%
TGCATTTAGACAAAACTAAG+AGG - chr2.4:5749829-5749848 None:intergenic 30.0%
TGGCATTCTTTATGTCATAT+AGG + chr2.4:5749687-5749706 MS.gene31080:intron 30.0%
TGTAAAAAAAGTTTGGAAGG+GGG + chr2.4:5750289-5750308 MS.gene31080:intron 30.0%
TGTACTGTTATAATTGGCAA+AGG - chr2.4:5751114-5751133 None:intergenic 30.0%
TTCAACATTGCAACCAATAT+GGG - chr2.4:5749477-5749496 None:intergenic 30.0%
! AACATTGTTGTTGTTGAAGT+TGG + chr2.4:5751202-5751221 MS.gene31080:intron 30.0%
! ATTGTTGTTCTACAAATGTC+TGG + chr2.4:5749194-5749213 MS.gene31080:CDS 30.0%
! GTATTTTCTAATCTACTCTC+TGG - chr2.4:5750752-5750771 None:intergenic 30.0%
! GTCTTAATGAAGTATCAACT+TGG - chr2.4:5748830-5748849 None:intergenic 30.0%
! TTGATTTGAATGAAGAGCAT+CGG + chr2.4:5748187-5748206 MS.gene31080:CDS 30.0%
!! ACAGTTTTTCGGTTTCAAAA+TGG + chr2.4:5750180-5750199 MS.gene31080:intron 30.0%
!! AGACTGTATTTACTTTTGTG+TGG + chr2.4:5748388-5748407 MS.gene31080:intron 30.0%
!! CAGCTTTAATAGCATCATTT+TGG - chr2.4:5748473-5748492 None:intergenic 30.0%
!! TTTTTGTTTGTGGCATGTTT+TGG + chr2.4:5748527-5748546 MS.gene31080:CDS 30.0%
!!! AATCTACTCTCTGGTTTTAA+TGG - chr2.4:5750743-5750762 None:intergenic 30.0%
!!! GCATTTTTAAGTTGATGAAG+AGG - chr2.4:5750570-5750589 None:intergenic 30.0%
AAAGCAAAACCAAGGAAACA+AGG + chr2.4:5750639-5750658 MS.gene31080:intron 35.0%
AAGCAAGTATAAGATACCGT+TGG + chr2.4:5748299-5748318 MS.gene31080:intron 35.0%
AAGCCATGTAGTAACTTTCT+TGG - chr2.4:5749382-5749401 None:intergenic 35.0%
ACAAAAACAATGACGTGTTC+CGG - chr2.4:5748514-5748533 None:intergenic 35.0%
ACCTTCTTCACCCAATATTA+TGG - chr2.4:5749351-5749370 None:intergenic 35.0%
AGAATGGAACACATCAAACT+CGG + chr2.4:5749115-5749134 MS.gene31080:CDS 35.0%
AGAGAAAACAGGATGGTAAA+TGG + chr2.4:5747863-5747882 MS.gene31080:CDS 35.0%
AGCAATTTGAAAAGAGATGG+TGG + chr2.4:5748643-5748662 MS.gene31080:CDS 35.0%
AGCCATGTAGTAACTTTCTT+GGG - chr2.4:5749381-5749400 None:intergenic 35.0%
AGCTTGAGTTCTTAAGTACA+TGG + chr2.4:5748565-5748584 MS.gene31080:CDS 35.0%
AGGAGCAATTTGAAAAGAGA+TGG + chr2.4:5748640-5748659 MS.gene31080:CDS 35.0%
ATCAGAACTACATGGTAGAA+TGG + chr2.4:5749099-5749118 MS.gene31080:CDS 35.0%
ATCCCAAGAAAGTTACTACA+TGG + chr2.4:5749376-5749395 MS.gene31080:CDS 35.0%
ATCCGACAGAATGTAATGAA+AGG - chr2.4:5749862-5749881 None:intergenic 35.0%
ATCGTTTGAGTGCAATACAT+CGG - chr2.4:5750941-5750960 None:intergenic 35.0%
ATGAAGGATATTTCTTTGGC+TGG + chr2.4:5748750-5748769 MS.gene31080:CDS 35.0%
ATGCTGTTGTCATGTGAATT+TGG + chr2.4:5750223-5750242 MS.gene31080:intron 35.0%
ATGTAGACGAGCCATAATAT+TGG + chr2.4:5749337-5749356 MS.gene31080:CDS 35.0%
ATTGTCATGCAAATGAGTTG+AGG - chr2.4:5751652-5751671 None:intergenic 35.0%
CAACAATTGCATGTCTACAT+AGG - chr2.4:5749181-5749200 None:intergenic 35.0%
CTATCAAAAGTTTCAGTTCG+AGG + chr2.4:5748995-5749014 MS.gene31080:CDS 35.0%
CTCCAAAATCTAAACCAGTA+AGG - chr2.4:5749935-5749954 None:intergenic 35.0%
CTCGTATATTGAGATTGAAC+AGG + chr2.4:5750589-5750608 MS.gene31080:intron 35.0%
CTTAAGAACTCAAGCTGTTT+CGG - chr2.4:5748561-5748580 None:intergenic 35.0%
CTTCAACATTGCAACCAATA+TGG - chr2.4:5749478-5749497 None:intergenic 35.0%
GAAAGTTACTACATGGCTTT+AGG + chr2.4:5749383-5749402 MS.gene31080:CDS 35.0%
GAACGCAATAGAAAAAGTCA+GGG - chr2.4:5749774-5749793 None:intergenic 35.0%
GTATCCTATTGGTGAAAACT+TGG + chr2.4:5749277-5749296 MS.gene31080:CDS 35.0%
GTCATTTAAAGAGACCTCAT+AGG + chr2.4:5750529-5750548 MS.gene31080:intron 35.0%
TAAAATGTCGTGTAACGTGA+TGG - chr2.4:5751177-5751196 None:intergenic 35.0%
TAACCATGGCTTTCAACAAA+TGG + chr2.4:5749738-5749757 MS.gene31080:CDS 35.0%
TATGCATGTGAAGAGAAAAC+AGG + chr2.4:5747852-5747871 MS.gene31080:CDS 35.0%
TATTGGTGAAAACTTGGAAG+AGG + chr2.4:5749283-5749302 MS.gene31080:CDS 35.0%
TCCATTTGCAGTAGTAATGA+CGG + chr2.4:5750907-5750926 MS.gene31080:intron 35.0%
TCCGACAGAATGTAATGAAA+GGG - chr2.4:5749861-5749880 None:intergenic 35.0%
TCTTGAAGATAGAGATGAAG+AGG + chr2.4:5748875-5748894 MS.gene31080:CDS 35.0%
TGAACGCAATAGAAAAAGTC+AGG - chr2.4:5749775-5749794 None:intergenic 35.0%
TGCTCGTATGTTATGTTCTT+GGG + chr2.4:5751236-5751255 MS.gene31080:intron 35.0%
TGTAGACGAGCCATAATATT+GGG + chr2.4:5749338-5749357 MS.gene31080:CDS 35.0%
TTGCTCGTATGTTATGTTCT+TGG + chr2.4:5751235-5751254 MS.gene31080:intron 35.0%
TTTGATGAGAACTTAGGAGT+TGG + chr2.4:5750475-5750494 MS.gene31080:intron 35.0%
! AAACATGGTTTGTAGCAATG+GGG + chr2.4:5748418-5748437 MS.gene31080:intron 35.0%
! AAAGAGTATGCAAAATCAGC+AGG + chr2.4:5747679-5747698 MS.gene31080:CDS 35.0%
! AAAGTTTCAGTTCGAGGTTT+TGG + chr2.4:5749001-5749020 MS.gene31080:CDS 35.0%
! AAGAGTATGCAAAATCAGCA+GGG + chr2.4:5747680-5747699 MS.gene31080:CDS 35.0%
! AAGTTTCAGTTCGAGGTTTT+GGG + chr2.4:5749002-5749021 MS.gene31080:CDS 35.0%
! ATACCATTTGTTGAAAGCCA+TGG - chr2.4:5749744-5749763 None:intergenic 35.0%
! ATCAAGTTGTGGAAACACTA+AGG + chr2.4:5751076-5751095 MS.gene31080:intron 35.0%
! ATTTTCTGAATCTGTTCTCG+AGG + chr2.4:5751449-5751468 MS.gene31080:intron 35.0%
! CAAACATGGTTTGTAGCAAT+GGG + chr2.4:5748417-5748436 MS.gene31080:intron 35.0%
! CAAGCTTGGTGAATATTGTT+GGG + chr2.4:5750329-5750348 MS.gene31080:intron 35.0%
! CCAAGCATCAAAATTTGCTT+TGG - chr2.4:5748904-5748923 None:intergenic 35.0%
! GCTTTAAGTAGGTATCCTAT+TGG + chr2.4:5749266-5749285 MS.gene31080:CDS 35.0%
! GTTGACTGGTTCATTTAGTT+TGG + chr2.4:5750000-5750019 MS.gene31080:intron 35.0%
! TACATGGCATGATAGCTTTA+TGG + chr2.4:5748581-5748600 MS.gene31080:CDS 35.0%
! TCAAGCTTGGTGAATATTGT+TGG + chr2.4:5750328-5750347 MS.gene31080:intron 35.0%
! TCTTCCAAGTTTTCACCAAT+AGG - chr2.4:5749284-5749303 None:intergenic 35.0%
!! CCAAAGCAAATTTTGATGCT+TGG + chr2.4:5748901-5748920 MS.gene31080:CDS 35.0%
!! CTTGCTTTACAACCGATTTT+CGG - chr2.4:5747832-5747851 None:intergenic 35.0%
!! TGTCTAAAAGTACTTATGGG+CGG + chr2.4:5750774-5750793 MS.gene31080:intron 35.0%
!! TGTTCTGGGTTGATTCTAAA+GGG + chr2.4:5748220-5748239 MS.gene31080:CDS 35.0%
!! TTGCTTTACAACCGATTTTC+GGG - chr2.4:5747831-5747850 None:intergenic 35.0%
!!! AACAGCAGTTGTTTTTCGAA+AGG - chr2.4:5748957-5748976 None:intergenic 35.0%
!!! ACAGCAGTTGTTTTTCGAAA+GGG - chr2.4:5748956-5748975 None:intergenic 35.0%
!!! CACGTCATTGTTTTTGTTTG+TGG + chr2.4:5748517-5748536 MS.gene31080:CDS 35.0%
!!! CTCATTTTTTTCGGAGTCAT+AGG + chr2.4:5747938-5747957 MS.gene31080:CDS 35.0%
!!! CTGCTCAAGCTCATTTTTTT+CGG + chr2.4:5747929-5747948 MS.gene31080:CDS 35.0%
!!! TCCCTTACTGGTTTAGATTT+TGG + chr2.4:5749930-5749949 MS.gene31080:intron 35.0%
AAAACCACTCGTAAGTGCAA+GGG + chr2.4:5750881-5750900 MS.gene31080:intron 40.0%
AACCAAGGAAACAAGGATGT+AGG + chr2.4:5750646-5750665 MS.gene31080:intron 40.0%
AATGCACATCCAAGCAATGT+TGG - chr2.4:5748357-5748376 None:intergenic 40.0%
ACAGAATGTAATGAAAGGGG+AGG - chr2.4:5749857-5749876 None:intergenic 40.0%
ACATGCACGATCATCATACA+AGG - chr2.4:5748715-5748734 None:intergenic 40.0%
ACCAGTAAGGGAAAACGAAA+AGG - chr2.4:5749922-5749941 None:intergenic 40.0%
ACGTGATGGTTGACATCATT+AGG - chr2.4:5751163-5751182 None:intergenic 40.0%
ATCATTTAATCTCCGGACCT+TGG - chr2.4:5749315-5749334 None:intergenic 40.0%
ATCGTCAACAAGATAAAGGG+CGG + chr2.4:5748076-5748095 MS.gene31080:CDS 40.0%
ATGAGACTTTGGGCTTAGAA+GGG + chr2.4:5751312-5751331 MS.gene31080:intron 40.0%
ATTCTTTCGATGCCCTAGAT+CGG - chr2.4:5747732-5747751 None:intergenic 40.0%
CAAAAACAATGACGTGTTCC+GGG - chr2.4:5748513-5748532 None:intergenic 40.0%
CATGTGAAGAGAAAACAGGA+TGG + chr2.4:5747856-5747875 MS.gene31080:CDS 40.0%
CATGTTCAACCAACATTGCT+TGG + chr2.4:5748345-5748364 MS.gene31080:intron 40.0%
CCCCTTTCATTACATTCTGT+CGG + chr2.4:5749857-5749876 MS.gene31080:intron 40.0%
CCGACAGAATGTAATGAAAG+GGG - chr2.4:5749860-5749879 None:intergenic 40.0%
CGAAAAAAATGAGCTTGAGC+AGG - chr2.4:5747931-5747950 None:intergenic 40.0%
CGTGATGGTTGACATCATTA+GGG - chr2.4:5751162-5751181 None:intergenic 40.0%
GCCATAATATTGGGTGAAGA+AGG + chr2.4:5749347-5749366 MS.gene31080:CDS 40.0%
GCTCGTATGTTATGTTCTTG+GGG + chr2.4:5751237-5751256 MS.gene31080:intron 40.0%
GTAATGATGTTCGGATGAGA+CGG + chr2.4:5747977-5747996 MS.gene31080:CDS 40.0%
GTATGATGATCGTGCATGTT+GGG + chr2.4:5748716-5748735 MS.gene31080:CDS 40.0%
TATCTCTGTGTGTTTGTGAC+AGG + chr2.4:5751524-5751543 MS.gene31080:intron 40.0%
TATGCAAAATCAGCAGGGTT+TGG + chr2.4:5747685-5747704 MS.gene31080:CDS 40.0%
TCAGAGACCTTATCGTTAGA+TGG - chr2.4:5749521-5749540 None:intergenic 40.0%
TCCGTCATTACTACTGCAAA+TGG - chr2.4:5750911-5750930 None:intergenic 40.0%
TCGGCTTAGAAATGTGTTCT+GGG + chr2.4:5748206-5748225 MS.gene31080:CDS 40.0%
TGTATGATGATCGTGCATGT+TGG + chr2.4:5748715-5748734 MS.gene31080:CDS 40.0%
TGTTGAAGGAAGTAAGGGAA+AGG + chr2.4:5749604-5749623 MS.gene31080:CDS 40.0%
TTCAAACAAGTGACAGGAAC+AGG - chr2.4:5749147-5749166 None:intergenic 40.0%
! AACATGGTTTGTAGCAATGG+GGG + chr2.4:5748419-5748438 MS.gene31080:intron 40.0%
! ACAAACGGTGTTAACGAAGA+AGG + chr2.4:5750434-5750453 MS.gene31080:intron 40.0%
! ACCTTTTCGTTTTCCCTTAC+TGG + chr2.4:5749918-5749937 MS.gene31080:intron 40.0%
! ATCGGCTTAGAAATGTGTTC+TGG + chr2.4:5748205-5748224 MS.gene31080:CDS 40.0%
! CAAACGGTGTTAACGAAGAA+GGG + chr2.4:5750435-5750454 MS.gene31080:intron 40.0%
! GACAGGAGCATACTGATATA+AGG + chr2.4:5751541-5751560 MS.gene31080:intron 40.0%
! GCAAACATGGTTTGTAGCAA+TGG + chr2.4:5748416-5748435 MS.gene31080:intron 40.0%
! GCAATGTTGGTTGAACATGA+TGG - chr2.4:5748344-5748363 None:intergenic 40.0%
! GTTATTTCAAAGCGGCATTG+TGG - chr2.4:5750721-5750740 None:intergenic 40.0%
! TCTAAAGGGTTGGAGGATTT+TGG + chr2.4:5748234-5748253 MS.gene31080:CDS 40.0%
! TTCCTACATCCTTGTTTCCT+TGG - chr2.4:5750651-5750670 None:intergenic 40.0%
!! ACTCCAACAAAAAGCACCAA+CGG - chr2.4:5748318-5748337 None:intergenic 40.0%
!! ATACCGTTGGTGCTTTTTGT+TGG + chr2.4:5748312-5748331 MS.gene31080:intron 40.0%
!! GTGTTCTGGGTTGATTCTAA+AGG + chr2.4:5748219-5748238 MS.gene31080:CDS 40.0%
!! TGCTTTACAACCGATTTTCG+GGG - chr2.4:5747830-5747849 None:intergenic 40.0%
!!! AGTTGTTTTTCGAAAGGGGA+AGG - chr2.4:5748951-5748970 None:intergenic 40.0%
!!! CAGCAGTTGTTTTTCGAAAG+GGG - chr2.4:5748955-5748974 None:intergenic 40.0%
AAGAGGTTCAATCCAAGGTC+CGG + chr2.4:5749300-5749319 MS.gene31080:CDS 45.0%
ACAAGATAAAGGGCGGAGTT+TGG + chr2.4:5748083-5748102 MS.gene31080:CDS 45.0%
AGAAGAATCGCACGTCTTGA+GGG - chr2.4:5750807-5750826 None:intergenic 45.0%
AGAAGCAATTGAGCATTCCC+CGG - chr2.4:5748117-5748136 None:intergenic 45.0%
AGGATGTAGGAATGCAGTAG+TGG + chr2.4:5750659-5750678 MS.gene31080:intron 45.0%
AGTTAAGTAGTCGCCGATCT+AGG + chr2.4:5747716-5747735 MS.gene31080:CDS 45.0%
ATACGAGTAAGAAGGCTGCA+AGG + chr2.4:5749552-5749571 MS.gene31080:CDS 45.0%
CAAAACCACTCGTAAGTGCA+AGG + chr2.4:5750880-5750899 MS.gene31080:intron 45.0%
CTTGGAAGAGGTTCAATCCA+AGG + chr2.4:5749295-5749314 MS.gene31080:CDS 45.0%
GAAAGGGGGACGAATCATTA+TGG - chr2.4:5751620-5751639 None:intergenic 45.0%
GAAGGAAGTAAGGGAAAGGT+TGG + chr2.4:5749608-5749627 MS.gene31080:intron 45.0%
GATGAGACTTTGGGCTTAGA+AGG + chr2.4:5751311-5751330 MS.gene31080:intron 45.0%
GCAACCAATATGGGTAGCTA+AGG - chr2.4:5749468-5749487 None:intergenic 45.0%
GCAGTAAGTGGTAGTAACCA+TGG + chr2.4:5749724-5749743 MS.gene31080:CDS 45.0%
GGGGAAGGTGACTTTAATTC+AGG - chr2.4:5748936-5748955 None:intergenic 45.0%
GTTAAGTAGTCGCCGATCTA+GGG + chr2.4:5747717-5747736 MS.gene31080:CDS 45.0%
TAAACTTTCAAAGCGCGAGC+CGG - chr2.4:5748784-5748803 None:intergenic 45.0%
TCATTATGGAACATAGCCGC+CGG - chr2.4:5751606-5751625 None:intergenic 45.0%
TGGTCAGTTAGAATCACCTG+CGG - chr2.4:5748453-5748472 None:intergenic 45.0%
TTGGAAGGGGGTCACTAAAT+GGG + chr2.4:5750301-5750320 MS.gene31080:intron 45.0%
TTGGGTGCCAACGTTTATGA+AGG + chr2.4:5748734-5748753 MS.gene31080:CDS 45.0%
TTGTGTGGCTACTGCAAACA+TGG + chr2.4:5748403-5748422 MS.gene31080:intron 45.0%
TTTGGAAGGGGGTCACTAAA+TGG + chr2.4:5750300-5750319 MS.gene31080:intron 45.0%
! AGCAGGTCTGAATCAAGTTG+TGG + chr2.4:5751065-5751084 MS.gene31080:intron 45.0%
! CTTGCACTTACGAGTGGTTT+TGG - chr2.4:5750882-5750901 None:intergenic 45.0%
! GGACAAACGTCGCTTTAAGT+AGG + chr2.4:5749255-5749274 MS.gene31080:CDS 45.0%
! GGTTGATTCTAAAGGGTTGG+AGG + chr2.4:5748227-5748246 MS.gene31080:CDS 45.0%
! TTGGTGGCAAGTTGAGAAGT+CGG - chr2.4:5751274-5751293 None:intergenic 45.0%
!! AGGAGGTGTTGAAGGAAGTA+AGG + chr2.4:5749598-5749617 MS.gene31080:CDS 45.0%
!! CTGGGTTGATTCTAAAGGGT+TGG + chr2.4:5748224-5748243 MS.gene31080:CDS 45.0%
!! GGAGGTGTTGAAGGAAGTAA+GGG + chr2.4:5749599-5749618 MS.gene31080:CDS 45.0%
!! TGAAAAGAGATGGTGGAAGC+TGG + chr2.4:5748650-5748669 MS.gene31080:CDS 45.0%
!! TTACAACCGATTTTCGGGGA+AGG - chr2.4:5747826-5747845 None:intergenic 45.0%
!! TTGCTTTAGAGCCTCGCTTA+AGG - chr2.4:5749411-5749430 None:intergenic 45.0%
!!! CGGAGTTTGGTTTTAGAACC+GGG + chr2.4:5748096-5748115 MS.gene31080:CDS 45.0%
!!! GCGGAGTTTGGTTTTAGAAC+CGG + chr2.4:5748095-5748114 MS.gene31080:CDS 45.0%
!!! GGAGTTTGGTTTTAGAACCG+GGG + chr2.4:5748097-5748116 MS.gene31080:CDS 45.0%
AAGAATTCGACTGCAGGTGG+TGG + chr2.4:5748003-5748022 MS.gene31080:CDS 50.0%
ACCACACGTCCATACCACAA+AGG - chr2.4:5751491-5751510 None:intergenic 50.0%
AGGAAGAATTCGACTGCAGG+TGG + chr2.4:5748000-5748019 MS.gene31080:CDS 50.0%
AGTAAGAAGGCTGCAAGGAC+TGG + chr2.4:5749557-5749576 MS.gene31080:CDS 50.0%
AGTTGAGGCTGCACTGTGAA+AGG - chr2.4:5751637-5751656 None:intergenic 50.0%
ATGATGTTCGGATGAGACGG+AGG + chr2.4:5747980-5747999 MS.gene31080:CDS 50.0%
CTAAATGGGGGAGTCAAGCT+TGG + chr2.4:5750315-5750334 MS.gene31080:intron 50.0%
GAAGAATCGCACGTCTTGAG+GGG - chr2.4:5750806-5750825 None:intergenic 50.0%
GAGAAGAATCGCACGTCTTG+AGG - chr2.4:5750808-5750827 None:intergenic 50.0%
GCAGCCTTAGCTACCCATAT+TGG + chr2.4:5749461-5749480 MS.gene31080:CDS 50.0%
GCTTGAGTGCCTTTGTGGTA+TGG + chr2.4:5751479-5751498 MS.gene31080:intron 50.0%
GTCATATAGGTGCCTCAGCT+TGG + chr2.4:5749700-5749719 MS.gene31080:intron 50.0%
GTTGAGGCTGCACTGTGAAA+GGG - chr2.4:5751636-5751655 None:intergenic 50.0%
TGACAGCTTGAGTGCCTTTG+TGG + chr2.4:5751474-5751493 MS.gene31080:intron 50.0%
TGGAAGGGGGTCACTAAATG+GGG + chr2.4:5750302-5750321 MS.gene31080:intron 50.0%
TTCAAAGCGGCATTGTGGAC+AGG - chr2.4:5750716-5750735 None:intergenic 50.0%
TTGAGGCTGCACTGTGAAAG+GGG - chr2.4:5751635-5751654 None:intergenic 50.0%
! ACGGTGTTAACGAAGAAGGG+AGG + chr2.4:5750438-5750457 MS.gene31080:intron 50.0%
! TGGTGGCAAGTTGAGAAGTC+GGG - chr2.4:5751273-5751292 None:intergenic 50.0%
!! AGGAGTTCAGGAGGTGTTGA+AGG + chr2.4:5749590-5749609 MS.gene31080:CDS 50.0%
!! GATGGTGGAAGCTGGTTGAT+AGG + chr2.4:5748658-5748677 MS.gene31080:CDS 50.0%
!! GGCTTTAGGTTCCTTAAGCG+AGG + chr2.4:5749397-5749416 MS.gene31080:CDS 50.0%
!! TCTTTGGCTGGCTTGTCTAC+CGG + chr2.4:5748762-5748781 MS.gene31080:CDS 50.0%
AGTGCCCTTGCACTTACGAG+TGG - chr2.4:5750888-5750907 None:intergenic 55.0%
CCACTTACTGCTCCAAGCTG+AGG - chr2.4:5749715-5749734 None:intergenic 55.0%
CCTCAGCTTGGAGCAGTAAG+TGG + chr2.4:5749712-5749731 MS.gene31080:CDS 55.0%
CGGAGGAAGAATTCGACTGC+AGG + chr2.4:5747997-5748016 MS.gene31080:CDS 55.0%
GCCTTTGTGGTATGGACGTG+TGG + chr2.4:5751487-5751506 MS.gene31080:intron 55.0%
GGAAGGGGGTCACTAAATGG+GGG + chr2.4:5750303-5750322 MS.gene31080:intron 55.0%
GGTGTAGCTGGTAGGAGTTC+AGG + chr2.4:5749578-5749597 MS.gene31080:CDS 55.0%
GTAGCTGGTAGGAGTTCAGG+AGG + chr2.4:5749581-5749600 MS.gene31080:CDS 55.0%
TGAGGCTGCACTGTGAAAGG+GGG - chr2.4:5751634-5751653 None:intergenic 55.0%
! CAAGGACTGGTGTAGCTGGT+AGG + chr2.4:5749570-5749589 MS.gene31080:CDS 55.0%
!!! GCTGTCGCTGCTGTTTTTCC+CGG + chr2.4:5748492-5748511 MS.gene31080:CDS 55.0%
CCGCGTCCTTCCCCGAAAAT+CGG + chr2.4:5747817-5747836 MS.gene31080:CDS 60.0%
! GCTGCAAGGACTGGTGTAGC+TGG + chr2.4:5749566-5749585 MS.gene31080:CDS 60.0%
!! CCGATTTTCGGGGAAGGACG+CGG - chr2.4:5747820-5747839 None:intergenic 60.0%
AATGGGGGAACGTGCTCCGC+AGG + chr2.4:5748434-5748453 MS.gene31080:intron 65.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 5747589 5751686 5747589 ID=MS.gene31080
chr2.4 mRNA 5747589 5751686 5747589 ID=MS.gene31080.t1;Parent=MS.gene31080
chr2.4 exon 5747589 5748255 5747589 ID=MS.gene31080.t1.exon1;Parent=MS.gene31080.t1
chr2.4 CDS 5747589 5748255 5747589 ID=cds.MS.gene31080.t1;Parent=MS.gene31080.t1
chr2.4 exon 5748490 5749625 5748490 ID=MS.gene31080.t1.exon2;Parent=MS.gene31080.t1
chr2.4 CDS 5748490 5749625 5748490 ID=cds.MS.gene31080.t1;Parent=MS.gene31080.t1
chr2.4 exon 5749709 5749759 5749709 ID=MS.gene31080.t1.exon3;Parent=MS.gene31080.t1
chr2.4 CDS 5749709 5749759 5749709 ID=cds.MS.gene31080.t1;Parent=MS.gene31080.t1
chr2.4 exon 5751546 5751686 5751546 ID=MS.gene31080.t1.exon4;Parent=MS.gene31080.t1
chr2.4 CDS 5751546 5751686 5751546 ID=cds.MS.gene31080.t1;Parent=MS.gene31080.t1
Gene Sequence

>MS.gene31080

ATGGATTCTTCTATTATTATGGCTAACACAATTCAAGACCCTAATAATGAAATGGAATTTGAATCACATGAAGCTGCATATTCATTTTACAAAGAGTATGCAAAATCAGCAGGGTTTGGTACTGCTAAGTTAAGTAGTCGCCGATCTAGGGCATCGAAAGAATTCATCGATGCGAAATTTTCATGTATTAGATATGGTAATAAACAACAATCGGATGACGCGATTAATCCGCGTCCTTCCCCGAAAATCGGTTGTAAAGCAAGTATGCATGTGAAGAGAAAACAGGATGGTAAATGGTATGTTTATAGTTTTGTTAAGGAGCATAATCACGAGCTTTTACCTGCTCAAGCTCATTTTTTTCGGAGTCATAGGAATAATGATACGTTGAGTAATGATGTTCGGATGAGACGGAGGAAGAATTCGACTGCAGGTGGTGGTAAGTTGTTTAGCGCGTATCAGAATATTGATTGTTTGGAGAATTTGATGAATCGTCAACAAGATAAAGGGCGGAGTTTGGTTTTAGAACCGGGGAATGCTCAATTGCTTCTTGAATTGTTTATGCATATGCAGGAAGAGAATCCGAAATTCTTTTATGCCATTGATTTGAATGAAGAGCATCGGCTTAGAAATGTGTTCTGGGTTGATTCTAAAGGGTTGGAGGATTTTGGTTATTTTTCTGATGTTGTTTCTTTTGACACAACGTATTTCACAAGCAAGTATAAGATACCGTTGGTGCTTTTTGTTGGAGTTAACCATCATGTTCAACCAACATTGCTTGGATGTGCATTGATAGCTGATGAGACTGTATTTACTTTTGTGTGGCTACTGCAAACATGGTTTGTAGCAATGGGGGAACGTGCTCCGCAGGTGATTCTAACTGACCAAAATGATGCTATTAAAGCTGCTGTCGCTGCTGTTTTTCCCGGAACACGTCATTGTTTTTGTTTGTGGCATGTTTTGGAAAAGATACCGAAACAGCTTGAGTTCTTAAGTACATGGCATGATAGCTTTATGGAAAAGTTCAACAAATGTATATATCAATCATGGACAGAGGAGCAATTTGAAAAGAGATGGTGGAAGCTGGTTGATAGGTTTAAACTTAGAGATGTTAAGTGGGTTCAAACCTTGTATGATGATCGTGCATGTTGGGTGCCAACGTTTATGAAGGATATTTCTTTGGCTGGCTTGTCTACCGGCTCGCGCTTTGAAAGTTTAAATTCTATTTTTGACAGATATATCCAAGTTGATACTTCATTAAGACAATTTATAGATCAGTACCAATTGATTCTTGAAGATAGAGATGAAGAGGAAGCCAAAGCAAATTTTGATGCTTGGCATGAAACACCTGAATTAAAGTCACCTTCCCCTTTCGAAAAACAACTGCTGTTAGTTTATACTCATGAAATCTATCAAAAGTTTCAGTTCGAGGTTTTGGGTGCTTCTGCTTGCCATCTAAAGAAAGAAAATGATGGCGTGATTACTACTTATGATGTGAAAGACTTCGAAAATAATCAGAACTACATGGTAGAATGGAACACATCAAACTCGGATATATCCTGTTCCTGTCACTTGTTTGAATATAAAGGTTACCTATGTAGACATGCAATTGTTGTTCTACAAATGTCTGGTGTTTTTAGCATCCCGCCAAAGTATATATTGAAAAGATGGACAAACGTCGCTTTAAGTAGGTATCCTATTGGTGAAAACTTGGAAGAGGTTCAATCCAAGGTCCGGAGATTAAATGATTTATGTAGACGAGCCATAATATTGGGTGAAGAAGGTTCTTTATCCCAAGAAAGTTACTACATGGCTTTAGGTTCCTTAAGCGAGGCTCTAAAGCAATGTACAAATTTGAACAACTCTGTTGAGAATGCAGCCTTAGCTACCCATATTGGTTGCAATGTTGAAGAAGAGTATCAATCTATTATACCATCTAACGATAAGGTCTCTGATCCGAAAATGAATACGAGTAAGAAGGCTGCAAGGACTGGTGTAGCTGGTAGGAGTTCAGGAGGTGTTGAAGGAAGTAAGGGAAAGGTTGGTTACTTTTGTGATTAATGATTGACCTCCTTGTACTATTTTAAAATTGTCTCTGACTTGGCATTCTTTATGTCATATAGGTGCCTCAGCTTGGAGCAGTAAGTGGTAGTAACCATGGCTTTCAACAAATGGTATGTTTATGCCCTGACTTTTTCTATTGCGTTCAAATTTACAATTATTAAGTTCTGATAATTTATCCTCTTAGTTTTGTCTAAATGCAACTAACCTCCCCTTTCATTACATTCTGTCGGATTTCTGCTAGAAGATAAAAAATTCTTTAATCTTTGAAACCTTTTCGTTTTCCCTTACTGGTTTAGATTTTGGAGAAATTAAAACGCTTAGATATTTGGAGGACTTATAATGCTTAATATGTTGACTGGTTCATTTAGTTTGGAAGACATATTTTTGTAGTTTTGAATTCATCAAAATTACTACTCCAGTTTTGTAGTTTTTAAGTTTATTTTGTCCAATTTTCATTGGTTTATTTGTCTCAAAGTTAATTGGTTTATATTATATAGCAGCTGTCATATGCTAAAATACTATCTTTGAAAACAGTTTTTCGGTTTCAAAATGGTTGCTATCACGTAGAAATTGATGCTGTTGTCATGTGAATTTGGAGAAAAACATGTTCGAGCGTTAATTCGTAGTCATGTTGTCAAGTGTAAAAAAAGTTTGGAAGGGGGTCACTAAATGGGGGAGTCAAGCTTGGTGAATATTGTTGGGAGCATGTGATTTTACTGTGATGTTTTGTCATATTTTTTTTTGAGGTTATTGTCATTATATTTTTTTGTATTTCAACGGAGAAACAAACGGTGTTAACGAAGAAGGGAGGTACTCATTTATGTATTTGATGAGAACTTAGGAGTTGGTGCATATTTTAAAACTAAAAGGGGTGTTTGTGTCATTTAAAGAGACCTCATAGGATGTCAATGTAATTTCCTCTTCATCAACTTAAAAATGCTCGTATATTGAGATTGAACAGGAAACCATAAACAATTTAAAATGAAACAAAAGCAAAACCAAGGAAACAAGGATGTAGGAATGCAGTAGTGGCGTATTGCACATAATTCACATGCATATTGAACCTGTCCACAATGCCGCTTTGAAATAACCATTAAAACCAGAGAGTAGATTAGAAAATACATTGTCTAAAAGTACTTATGGGCGGAGTGTTCCCCTCAAGACGTGCGATTCTTCTCCAACAAAAATGCTTTATTTCTAAGCTAGATTTACACATAATATTTGTTGCGCCAAAACCACTCGTAAGTGCAAGGGCACTCCATTTGCAGTAGTAATGACGGACAGAAGTCCGATGTATTGCACTCAAACGATTAAATAGTAATTACTAAATTAATCAAGCTTCTATATTATTTAGACCATTGAAGTTATTGTTTAAATCTTTGGAATCAGAGCAACAAAGAACAAATCAATTAGTAAGCAGGTCTGAATCAAGTTGTGGAAACACTAAGGTGTTTCGTTTCACCTTTGCCAATTATAACAGTACATTCTAATTAATCTACGATTTATGTTCCCTAATGATGTCAACCATCACGTTACACGACATTTTATTGTGAACATTGTTGTTGTTGAAGTTGGTTATTTTGCTTTGCTCGTATGTTATGTTCTTGGGGAGTTTCAATACCCGACTTCTCAACTTGCCACCAATGACAAATATCTGTTAAGATGAGACTTTGGGCTTAGAAGGGAAAATAAAGAAATGCAGGCTAGTTTAGTTTCTCGACCCAATATATAGTAGTTTAGGCTAGACATTAATTTTTGGCAAACAATCAATATCTGTATTCTTGAATCTTGACGAGATCATTTTCTGAATCTGTTCTCGAGGTTTGACAGCTTGAGTGCCTTTGTGGTATGGACGTGTGGTTCCAAATTTTTAATATCTCTGTGTGTTTGTGACAGGAGCATACTGATATAAGGTCACACAATGTCATGCCAGTGCAGTTTCACAGCATGGTGCCGGCGGCTATGTTCCATAATGATTCGTCCCCCTTTCACAGTGCAGCCTCAACTCATTTGCATGACAATCAACTTCCTCCTTAA
Protein sequence

>MS.gene31080.t1

MDSSIIMANTIQDPNNEMEFESHEAAYSFYKEYAKSAGFGTAKLSSRRSRASKEFIDAKFSCIRYGNKQQSDDAINPRPSPKIGCKASMHVKRKQDGKWYVYSFVKEHNHELLPAQAHFFRSHRNNDTLSNDVRMRRRKNSTAGGGKLFSAYQNIDCLENLMNRQQDKGRSLVLEPGNAQLLLELFMHMQEENPKFFYAIDLNEEHRLRNVFWVDSKGLEDFAAVAAVFPGTRHCFCLWHVLEKIPKQLEFLSTWHDSFMEKFNKCIYQSWTEEQFEKRWWKLVDRFKLRDVKWVQTLYDDRACWVPTFMKDISLAGLSTGSRFESLNSIFDRYIQVDTSLRQFIDQYQLILEDRDEEEAKANFDAWHETPELKSPSPFEKQLLLVYTHEIYQKFQFEVLGASACHLKKENDGVITTYDVKDFENNQNYMVEWNTSNSDISCSCHLFEYKGYLCRHAIVVLQMSGVFSIPPKYILKRWTNVALSRYPIGENLEEVQSKVRRLNDLCRRAIILGEEGSLSQESYYMALGSLSEALKQCTNLNNSVENAALATHIGCNVEEEYQSIIPSNDKVSDPKMNTSKKAARTGVAGRSSGGVEGSKGKVPQLGAVSGSNHGFQQMEHTDIRSHNVMPVQFHSMVPAAMFHNDSSPFHSAASTHLHDNQLPP