AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene31132

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31132.t1 MTR_2g097390 93.808 646 37 1 1 646 1 643 0.0 1217
MS.gene31132.t1 MTR_2g097310 83.667 649 94 4 1 646 1 640 0.0 1088
MS.gene31132.t1 MTR_2g097350 82.743 649 102 3 1 646 1 642 0.0 1049
MS.gene31132.t1 MTR_2g097380 80.972 494 50 3 153 646 114 563 0.0 821
MS.gene31132.t1 MTR_4g064120 42.448 629 306 16 46 646 28 628 2.61e-156 464
MS.gene31132.t1 MTR_2g097410 51.678 447 198 9 213 646 302 743 2.33e-153 461
MS.gene31132.t1 MTR_4g064180 41.614 632 307 15 46 646 28 628 8.68e-152 452
MS.gene31132.t1 MTR_7g062120 52.835 388 177 3 263 646 357 742 7.86e-150 451
MS.gene31132.t1 MTR_4g064150 48.747 439 209 7 222 646 299 735 9.92e-147 443
MS.gene31132.t1 MTR_2g097467 39.297 626 331 15 50 646 51 656 1.84e-141 427
MS.gene31132.t1 MTR_4g064200 46.072 471 227 7 196 646 189 652 6.39e-140 423
MS.gene31132.t1 MTR_2g097473 39.326 623 335 15 50 646 51 656 1.00e-139 422
MS.gene31132.t1 MTR_2g097463 46.882 433 217 5 222 646 230 657 8.40e-138 417
MS.gene31132.t1 MTR_5g097480 31.200 375 249 4 274 643 173 543 8.04e-63 218
MS.gene31132.t1 MTR_3g056110 29.026 534 330 16 133 643 34 541 3.19e-60 211
MS.gene31132.t1 MTR_4g133660 30.079 379 253 5 273 644 170 543 3.19e-59 208
MS.gene31132.t1 MTR_2g082090 31.830 377 244 4 274 643 208 578 1.70e-58 207
MS.gene31132.t1 MTR_2g026250 29.765 383 258 5 269 643 216 595 4.37e-58 206
MS.gene31132.t1 MTR_3g089055 29.570 372 255 3 276 643 201 569 6.56e-55 197
MS.gene31132.t1 MTR_3g089055 29.570 372 255 3 276 643 196 564 6.63e-55 196
MS.gene31132.t1 MTR_5g094450 30.526 380 249 8 274 643 157 531 1.37e-54 195
MS.gene31132.t1 MTR_1g029420 29.839 372 254 3 276 643 223 591 7.99e-54 194
MS.gene31132.t1 MTR_7g027190 31.218 394 232 8 273 643 295 672 1.27e-53 195
MS.gene31132.t1 MTR_4g104020 28.272 382 253 8 275 643 146 519 1.27e-45 170
MS.gene31132.t1 MTR_8g442410 27.949 390 247 9 273 643 150 524 6.47e-45 168
MS.gene31132.t1 MTR_3g065980 31.122 392 240 8 268 643 162 539 1.37e-44 167
MS.gene31132.t1 MTR_4g102790 26.684 386 259 9 274 645 288 663 1.72e-44 169
MS.gene31132.t1 MTR_7g074650 30.959 365 238 8 282 645 442 793 4.18e-44 169
MS.gene31132.t1 MTR_5g009080 25.339 442 264 9 263 643 41 477 8.69e-40 152
MS.gene31132.t1 MTR_3g022830 24.578 415 258 7 279 643 26 435 4.75e-38 147
MS.gene31132.t1 MTR_4g076140 24.943 437 263 9 267 643 37 468 1.18e-37 146
MS.gene31132.t1 MTR_4g097080 24.742 388 256 10 278 645 133 504 4.26e-34 136
MS.gene31132.t1 MTR_2g089100 26.942 412 246 10 275 646 49 445 1.74e-32 131
MS.gene31132.t1 MTR_4g077760 27.895 380 247 10 275 643 191 554 2.19e-32 132
MS.gene31132.t1 MTR_2g034260 24.615 390 270 8 270 643 202 583 1.10e-31 130
MS.gene31132.t1 MTR_2g034250 24.742 388 273 6 270 643 202 584 1.40e-31 130
MS.gene31132.t1 MTR_5g015490 25.131 382 268 10 278 645 114 491 4.09e-31 127
MS.gene31132.t1 MTR_1g096030 27.411 394 243 13 278 643 80 458 4.76e-31 127
MS.gene31132.t1 MTR_1g069725 27.704 379 252 11 270 643 101 462 3.44e-30 124
MS.gene31132.t1 MTR_7g109580 25.789 380 263 9 273 643 184 553 6.43e-30 125
MS.gene31132.t1 MTR_2g034280 23.834 386 276 7 270 643 195 574 5.72e-29 122
MS.gene31132.t1 MTR_4g076020 27.273 374 233 12 282 646 88 431 6.35e-29 120
MS.gene31132.t1 MTR_7g057230 26.804 291 194 6 363 643 375 656 2.35e-28 120
MS.gene31132.t1 MTR_6g047750 25.656 343 231 9 313 643 331 661 8.88e-26 113
MS.gene31132.t1 MTR_5g058860 26.343 391 250 16 278 643 119 496 1.09e-23 105
MS.gene31132.t1 MTR_1g086970 24.605 443 279 17 231 643 60 477 2.21e-23 104
MS.gene31132.t1 MTR_2g099110 26.667 390 253 10 278 645 74 452 2.98e-22 100
MS.gene31132.t1 MTR_4g095500 22.323 439 280 10 260 646 41 470 9.38e-22 99.4
MS.gene31132.t1 MTR_3g072710 24.566 403 254 14 273 643 115 499 1.87e-21 98.6
MS.gene31132.t1 MTR_5g015950 21.127 426 273 9 275 646 32 448 1.76e-20 95.1
MS.gene31132.t1 MTR_3g053270 21.127 426 273 9 275 646 32 448 1.76e-20 95.1
MS.gene31132.t1 MTR_0092s0100 24.603 378 249 11 279 643 376 730 6.75e-18 88.2
MS.gene31132.t1 MTR_0092s0100 24.603 378 249 11 279 643 373 727 7.02e-18 88.2
MS.gene31132.t1 MTR_8g093070 23.058 399 272 14 261 646 122 498 8.07e-18 87.4
MS.gene31132.t1 MTR_4g026485 24.267 375 253 12 279 643 270 623 2.12e-17 86.7
MS.gene31132.t1 MTR_2g097370 90.625 32 3 0 62 93 1 32 7.88e-14 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31132.t1 AT2G29060 42.326 619 315 11 50 645 57 656 2.21e-167 494
MS.gene31132.t1 AT2G29060 41.213 643 325 13 50 645 57 693 1.21e-164 488
MS.gene31132.t1 AT1G07530 51.410 461 204 7 203 646 310 767 5.28e-163 486
MS.gene31132.t1 AT1G07520 48.322 447 215 8 211 646 90 531 8.95e-143 426
MS.gene31132.t1 AT1G07520 48.322 447 215 8 211 646 90 531 8.95e-143 426
MS.gene31132.t1 AT2G29065 41.531 614 325 16 50 646 34 630 5.98e-142 427
MS.gene31132.t1 AT1G07520 48.322 447 215 8 211 646 254 695 3.14e-141 428
MS.gene31132.t1 AT1G07520 48.322 447 215 8 211 646 255 696 3.17e-141 428
MS.gene31132.t1 AT2G37650 48.698 384 189 4 266 645 323 702 8.14e-127 391
MS.gene31132.t1 AT2G37650 48.698 384 189 4 266 645 335 714 9.83e-127 391
MS.gene31132.t1 AT3G46600 43.387 431 233 5 220 646 126 549 9.86e-120 367
MS.gene31132.t1 AT3G46600 43.794 427 229 5 224 646 162 581 1.45e-119 368
MS.gene31132.t1 AT5G59450 40.789 456 259 6 201 646 146 600 1.43e-116 361
MS.gene31132.t1 AT3G46600 46.011 376 196 4 275 646 1 373 1.40e-114 348
MS.gene31132.t1 AT5G48150 33.333 375 242 5 274 643 118 489 1.38e-65 224
MS.gene31132.t1 AT5G48150 33.333 375 242 5 274 643 118 489 1.38e-65 224
MS.gene31132.t1 AT5G48150 33.333 375 242 5 274 643 118 489 1.38e-65 224
MS.gene31132.t1 AT5G48150 33.333 375 242 5 274 643 118 489 1.38e-65 224
MS.gene31132.t1 AT2G04890 31.818 374 241 4 274 643 49 412 6.82e-65 219
MS.gene31132.t1 AT1G50600 31.343 402 256 6 251 643 135 525 7.28e-61 212
MS.gene31132.t1 AT1G50600 31.343 402 256 6 251 643 206 596 2.15e-60 213
MS.gene31132.t1 AT1G50600 31.343 402 256 6 251 643 210 600 2.43e-60 212
MS.gene31132.t1 AT1G21450 27.788 529 351 13 128 643 82 592 1.71e-55 199
MS.gene31132.t1 AT4G17230 29.178 377 260 3 273 645 153 526 1.62e-49 181
MS.gene31132.t1 AT4G17230 29.178 377 260 3 273 645 153 526 1.62e-49 181
MS.gene31132.t1 AT3G54220 31.818 396 253 9 254 645 269 651 8.60e-47 176
MS.gene31132.t1 AT2G01570 30.829 386 238 11 268 643 214 580 7.97e-45 169
MS.gene31132.t1 AT1G66350 30.809 383 236 10 268 643 145 505 2.62e-44 166
MS.gene31132.t1 AT3G03450 28.238 386 253 8 268 643 173 544 1.92e-43 164
MS.gene31132.t1 AT1G14920 29.058 382 250 8 268 643 162 528 2.20e-43 164
MS.gene31132.t1 AT5G17490 26.720 378 259 7 270 643 152 515 7.56e-41 156
MS.gene31132.t1 AT1G50420 22.940 449 278 9 257 643 36 478 6.17e-36 141
MS.gene31132.t1 AT1G63100 28.453 362 234 10 266 618 244 589 4.21e-34 138
MS.gene31132.t1 AT1G63100 28.453 362 234 10 266 618 269 614 4.54e-34 138
MS.gene31132.t1 AT5G66770 29.333 375 237 12 282 643 224 583 5.79e-34 137
MS.gene31132.t1 AT5G41920 29.793 386 234 14 272 643 37 399 2.96e-32 129
MS.gene31132.t1 AT4G37650 25.000 360 236 9 277 611 145 495 1.04e-30 127
MS.gene31132.t1 AT3G50650 26.171 363 235 10 298 643 195 541 1.88e-28 120
MS.gene31132.t1 AT5G52510 24.289 387 261 9 276 643 266 639 9.65e-28 119
MS.gene31132.t1 AT3G49950 24.079 407 283 9 258 645 10 409 1.67e-24 107
MS.gene31132.t1 AT4G08250 25.000 392 264 13 270 643 97 476 2.52e-24 107
MS.gene31132.t1 AT1G55580 25.845 414 252 11 275 643 41 444 4.14e-24 106
MS.gene31132.t1 AT3G60630 28.571 210 125 8 349 544 327 525 1.63e-11 67.8

Find 110 sgRNAs with CRISPR-Local

Find 131 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAGAAAATGGAGGAGTATT+TGG 0.132396 2.4:+6358632 None:intergenic
TTCACTCTCTCCAATGAATT+TGG 0.151096 2.4:+6357867 None:intergenic
AGTCTTCACATTATAGATTT+TGG 0.160984 2.4:-6357984 MS.gene31132:CDS
ATGCTATGAATAAATATATT+TGG 0.170780 2.4:+6357618 None:intergenic
AATCTTCTTCCATGAGAAAT+TGG 0.197500 2.4:+6358954 None:intergenic
CAAAGATTGGCACATTTCTT+TGG 0.204755 2.4:-6358191 MS.gene31132:CDS
GAATTCACTTCAACTGTTCT+TGG 0.234393 2.4:-6359082 MS.gene31132:CDS
TTTCTCAATGTGTCACAAAA+TGG 0.237333 2.4:-6358509 MS.gene31132:CDS
GTGAAGACTTTCTGTTTCTT+TGG 0.238565 2.4:+6357998 None:intergenic
CAAACAGATTCTATGGTTCT+AGG 0.245218 2.4:-6358358 MS.gene31132:CDS
TTTATTCATAGCATTGTTAA+TGG 0.248656 2.4:-6357609 MS.gene31132:CDS
TTCTATTTGGTTCTTCTTGT+TGG 0.254378 2.4:-6357221 MS.gene31132:CDS
ACAAAGTTATTAGGAGAAAA+TGG 0.257230 2.4:+6358620 None:intergenic
TCTTAATCTGGTTTAATAAC+TGG 0.258792 2.4:+6358243 None:intergenic
GATTGGGAAACAAAGTTATT+AGG 0.262305 2.4:+6358611 None:intergenic
CATATATGCAATGGCATTCT+AGG 0.264227 2.4:-6357389 MS.gene31132:CDS
TTTGATAAAGTACTAGTTCT+TGG 0.268048 2.4:-6358440 MS.gene31132:CDS
TGAAATATCATTACAAGAAA+TGG 0.276552 2.4:+6358274 None:intergenic
ATTATTGAAAGAAGGGAAAA+AGG 0.276813 2.4:+6358580 None:intergenic
ATTCACTCTTACTTTGTTCA+TGG 0.287286 2.4:+6359101 None:intergenic
AAACTATAATCACTGAAGTT+AGG 0.291953 2.4:+6358734 None:intergenic
GAATCCGAGAGGTGCTGTTT+TGG 0.303272 2.4:-6357661 MS.gene31132:CDS
GAGAAAATGGAGGAGTATTT+GGG 0.303863 2.4:+6358633 None:intergenic
TGTTTGAGGTGAAGGAAAAT+TGG 0.305166 2.4:-6357269 MS.gene31132:CDS
TGGAAGGGTAGAATTCTATT+TGG 0.313548 2.4:-6357234 MS.gene31132:CDS
GAGAAGAATGTGTCTTAATC+TGG 0.320149 2.4:+6358231 None:intergenic
CATTCTAGGCACATGGGAAA+TGG 0.344559 2.4:-6357375 MS.gene31132:CDS
GAATTATCAAACAGATTCTA+TGG 0.344805 2.4:-6358365 MS.gene31132:CDS
GATAGATCTTCCAAATTCAT+TGG 0.350686 2.4:-6357877 MS.gene31132:CDS
TTGGGAAAGTATCAAAGTTC+AGG 0.363131 2.4:-6357769 MS.gene31132:CDS
TGGGTTTAGATCTTTGAAAC+TGG 0.374406 2.4:-6357355 MS.gene31132:CDS
TCCTCAAACATCAACCTAAC+AGG 0.379387 2.4:+6357483 None:intergenic
GGAGTATTTGGGAGTAGAAA+AGG 0.380526 2.4:+6358644 None:intergenic
TTGATAATTCTTCATTCTGC+TGG 0.383176 2.4:+6358378 None:intergenic
ACCTGATGAGACAGTTGTTT+CGG 0.393796 2.4:-6357685 MS.gene31132:CDS
TAGCCTGTGAAGGCTGTGAT+AGG 0.399278 2.4:-6357428 MS.gene31132:CDS
GCAATACCATTGTACTCAAA+AGG 0.401991 2.4:+6357798 None:intergenic
CTTGGTACTAAATTCACAAA+AGG 0.404219 2.4:-6358422 MS.gene31132:CDS
GTTGAAGAGCATAGGAATCT+TGG 0.409119 2.4:+6358909 None:intergenic
GATGTTTGAGGAAGACGTTT+GGG 0.411150 2.4:-6357472 MS.gene31132:CDS
GAGTGAATGAAACAGGTCTT+AGG 0.420195 2.4:-6357851 MS.gene31132:CDS
GAAAGAGCATTATTGAAAGA+AGG 0.427388 2.4:+6358572 None:intergenic
GAGTATTTGGGAGTAGAAAA+GGG 0.432472 2.4:+6358645 None:intergenic
TTGGAGAGAGTGAATGAAAC+AGG 0.438708 2.4:-6357858 MS.gene31132:CDS
ATGAGGAAGTTGTGGATTTG+AGG 0.441434 2.4:-6358322 MS.gene31132:CDS
AACTATAATCACTGAAGTTA+GGG 0.442010 2.4:+6358735 None:intergenic
AAAGAGCATTATTGAAAGAA+GGG 0.443472 2.4:+6358573 None:intergenic
CTCTTACTTTGTTCATGGAG+TGG 0.447982 2.4:+6359106 None:intergenic
TGATGTTTGAGGAAGACGTT+TGG 0.448987 2.4:-6357473 MS.gene31132:CDS
AAGACACATTCTTCTCCAAC+AGG 0.450645 2.4:-6358224 MS.gene31132:CDS
GAAGCACGTTTAGCAGGAAC+TGG 0.455780 2.4:-6358158 MS.gene31132:CDS
ATTCTAGGCACATGGGAAAT+GGG 0.456200 2.4:-6357374 MS.gene31132:CDS
GGTTATGGTTTCAAGTGGCT+TGG 0.456727 2.4:-6357957 MS.gene31132:CDS
CTTTGAGTTCCATCACCTGT+TGG 0.456885 2.4:+6358209 None:intergenic
TATCCCTGTGATCCTCAACT+TGG 0.460979 2.4:+6357896 None:intergenic
GCAGATTATAAATAAATTGA+AGG 0.461295 2.4:-6357328 MS.gene31132:CDS
TCCTGTTAGGTTGATGTTTG+AGG 0.467599 2.4:-6357484 MS.gene31132:CDS
TGCCATTGCATATATGTCTC+TGG 0.473005 2.4:+6357396 None:intergenic
TCCGAAACAACTGTCTCATC+AGG 0.480396 2.4:+6357684 None:intergenic
ACTCTTCATGCTCATTGTGT+TGG 0.485254 2.4:+6359002 None:intergenic
CCACCCAAGTTGAGGATCAC+AGG 0.486139 2.4:-6357900 MS.gene31132:CDS
GTAGGAGGAAGAGGATGAAG+AGG 0.486409 2.4:+6358848 None:intergenic
GCAGCAGCAGTAGGAGGAAG+AGG 0.487800 2.4:+6358839 None:intergenic
TATCGTTTATCGAAAAGATC+TGG 0.498438 2.4:-6357927 MS.gene31132:CDS
GCACTTGAAGCACGTTTAGC+AGG 0.499951 2.4:-6358164 MS.gene31132:CDS
TTGAATGTGAGGATCCTGTT+AGG 0.507367 2.4:-6357497 MS.gene31132:CDS
AAAATTGGATGCTACAAGGT+TGG 0.509330 2.4:-6357254 MS.gene31132:CDS
TTGGTACTAAATTCACAAAA+GGG 0.510419 2.4:-6358421 MS.gene31132:CDS
AACCCTATCACAGCCTTCAC+AGG 0.513850 2.4:+6357425 None:intergenic
GTTGATATAACCAAGAGCAT+TGG 0.514520 2.4:+6358976 None:intergenic
CATTCTTCTCCAACAGGTGA+TGG 0.515669 2.4:-6358218 MS.gene31132:CDS
GAAGAGTCATCCAATGCTCT+TGG 0.522768 2.4:-6358986 MS.gene31132:CDS
CTAGTTGTGAAGGTCTATGC+TGG 0.523269 2.4:+6357195 None:intergenic
TTATATCAACCAATTTCTCA+TGG 0.530130 2.4:-6358963 MS.gene31132:CDS
AAGAAGTTATGAGGAAGTTG+TGG 0.533441 2.4:-6358330 MS.gene31132:CDS
TACTCATGCTGGATGGAAGA+AGG 0.547361 2.4:+6358785 None:intergenic
GGCATGCTGATGAATAAACT+TGG 0.547453 2.4:+6358072 None:intergenic
TTGGATGCTACAAGGTTGGA+AGG 0.548278 2.4:-6357250 MS.gene31132:CDS
TAGGAGACAAAGAAGTTATG+AGG 0.549337 2.4:-6358339 MS.gene31132:CDS
CAGATTATAAATAAATTGAA+GGG 0.550775 2.4:-6357327 MS.gene31132:CDS
ATTCATAGCATTGTTAATGG+TGG 0.554304 2.4:-6357606 MS.gene31132:CDS
ATCCCTGTGATCCTCAACTT+GGG 0.558025 2.4:+6357897 None:intergenic
AGGTGATGGAACTCAAAGAT+TGG 0.559955 2.4:-6358204 MS.gene31132:CDS
AGCCTGTGAAGGCTGTGATA+GGG 0.569110 2.4:-6357427 MS.gene31132:CDS
GCAATGGCATTCTAGGCACA+TGG 0.578087 2.4:-6357382 MS.gene31132:CDS
ATGTTTGAGGAAGACGTTTG+GGG 0.578234 2.4:-6357471 MS.gene31132:CDS
AAATCCAAAACAGCACCTCT+CGG 0.585044 2.4:+6357657 None:intergenic
AATTTGTTATAGATGGACAA+AGG 0.588146 2.4:-6359139 None:intergenic
TATATGTGGATCAAGTTCAA+AGG 0.594302 2.4:+6358691 None:intergenic
GGCCAGAGACATATATGCAA+TGG 0.597570 2.4:-6357398 MS.gene31132:CDS
CTCAGTAAGTTGAAGAGCAT+AGG 0.600415 2.4:+6358901 None:intergenic
CACCCAAGTTGAGGATCACA+GGG 0.601694 2.4:-6357899 MS.gene31132:CDS
CGTTTATCGAAAAGATCTGG+TGG 0.602238 2.4:-6357924 MS.gene31132:CDS
AAGGAAAATTGGATGCTACA+AGG 0.606455 2.4:-6357258 MS.gene31132:CDS
CCTGTGATCCTCAACTTGGG+TGG 0.609546 2.4:+6357900 None:intergenic
GTGTTGGTTATGGTTTCAAG+TGG 0.612035 2.4:-6357962 MS.gene31132:CDS
TGTTTGAGGAAGACGTTTGG+GGG 0.623857 2.4:-6357470 MS.gene31132:CDS
TAGTTGTATGAGTCTATATG+TGG 0.625308 2.4:+6358677 None:intergenic
CAATGGCATTCTAGGCACAT+GGG 0.638518 2.4:-6357381 MS.gene31132:CDS
TGGATGCTACAAGGTTGGAA+GGG 0.641743 2.4:-6357249 MS.gene31132:CDS
GTAAGCTTTGACCATATCTG+CGG 0.645774 2.4:+6358094 None:intergenic
AAGGCTGTGATAGGGTTCAG+AGG 0.656517 2.4:-6357419 MS.gene31132:CDS
CTGGTGGGCCACCCAAGTTG+AGG 0.658509 2.4:-6357908 MS.gene31132:CDS
ATGTGGATCAAGTTCAAAGG+AGG 0.665342 2.4:+6358694 None:intergenic
ATGAATGTTATAGCCTGTGA+AGG 0.667099 2.4:-6357438 MS.gene31132:CDS
TTGTTGATACTCATGCTGGA+TGG 0.669072 2.4:+6358778 None:intergenic
TAACAACAATGTTGAATGTG+AGG 0.669855 2.4:-6357508 MS.gene31132:CDS
AAGTTATTAGGAGAAAATGG+AGG 0.670375 2.4:+6358623 None:intergenic
GTTTATCGAAAAGATCTGGT+GGG 0.689733 2.4:-6357923 MS.gene31132:CDS
GTTGTTTCGGAGAATCCGAG+AGG 0.745211 2.4:-6357672 MS.gene31132:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGCTATGAATAAATATATT+TGG + chr2.4:6358721-6358740 None:intergenic 15.0%
!! CAGATTATAAATAAATTGAA+GGG - chr2.4:6359009-6359028 MS.gene31132:CDS 15.0%
!!! TTTTATAAACAAATTTCTCA+TGG - chr2.4:6357277-6357296 MS.gene31132:CDS 15.0%
!! GCAGATTATAAATAAATTGA+AGG - chr2.4:6359008-6359027 MS.gene31132:CDS 20.0%
!! TGAAATATCATTACAAGAAA+TGG + chr2.4:6358065-6358084 None:intergenic 20.0%
!! TTTATTCATAGCATTGTTAA+TGG - chr2.4:6358727-6358746 MS.gene31132:CDS 20.0%
!!! ATAATTGCAAGTTTTTCAAA+AGG + chr2.4:6358289-6358308 None:intergenic 20.0%
!!! TAATTGCAAGTTTTTCAAAA+GGG + chr2.4:6358288-6358307 None:intergenic 20.0%
! AAACTATAATCACTGAAGTT+AGG + chr2.4:6357605-6357624 None:intergenic 25.0%
! AACTATAATCACTGAAGTTA+GGG + chr2.4:6357604-6357623 None:intergenic 25.0%
! ACAAAGTTATTAGGAGAAAA+TGG + chr2.4:6357719-6357738 None:intergenic 25.0%
! ACAATGGTATTGCAAAAAAT+TGG - chr2.4:6358548-6358567 MS.gene31132:CDS 25.0%
! ATTATTGAAAGAAGGGAAAA+AGG + chr2.4:6357759-6357778 None:intergenic 25.0%
! CAATGGTATTGCAAAAAATT+GGG - chr2.4:6358549-6358568 MS.gene31132:CDS 25.0%
! GAATTATCAAACAGATTCTA+TGG - chr2.4:6357971-6357990 MS.gene31132:CDS 25.0%
! TTAAACCTTGTCACAAAAAA+AGG + chr2.4:6358766-6358785 None:intergenic 25.0%
! TTATATCAACCAATTTCTCA+TGG - chr2.4:6357373-6357392 MS.gene31132:CDS 25.0%
! TTGGTACTAAATTCACAAAA+GGG - chr2.4:6357915-6357934 MS.gene31132:CDS 25.0%
!! AGTCTTCACATTATAGATTT+TGG - chr2.4:6358352-6358371 MS.gene31132:CDS 25.0%
!! TAATAGTGATTTTCTGTTTG+AGG - chr2.4:6359053-6359072 MS.gene31132:CDS 25.0%
!! TCTTAATCTGGTTTAATAAC+TGG + chr2.4:6358096-6358115 None:intergenic 25.0%
!! TTTGATAAAGTACTAGTTCT+TGG - chr2.4:6357896-6357915 MS.gene31132:CDS 25.0%
!!! AAAGAGCATTATTGAAAGAA+GGG + chr2.4:6357766-6357785 None:intergenic 25.0%
!!! AAGTTCAGGATTTTAAGATT+AGG - chr2.4:6358581-6358600 MS.gene31132:CDS 25.0%
!!! TGTTTTGGATTTGATTAAGA+AGG - chr2.4:6358690-6358709 MS.gene31132:CDS 25.0%
!!! TTTTTTTGTGACAAGGTTTA+AGG - chr2.4:6358765-6358784 MS.gene31132:CDS 25.0%
AAGTTATTAGGAGAAAATGG+AGG + chr2.4:6357716-6357735 None:intergenic 30.0%
AATCTTCTTCCATGAGAAAT+TGG + chr2.4:6357385-6357404 None:intergenic 30.0%
ATTCACTCTTACTTTGTTCA+TGG + chr2.4:6357238-6357257 None:intergenic 30.0%
ATTCATAGCATTGTTAATGG+TGG - chr2.4:6358730-6358749 MS.gene31132:CDS 30.0%
CTTGGTACTAAATTCACAAA+AGG - chr2.4:6357914-6357933 MS.gene31132:CDS 30.0%
GAAAAAGGTTGAAGTAGATT+GGG + chr2.4:6357744-6357763 None:intergenic 30.0%
GATAGATCTTCCAAATTCAT+TGG - chr2.4:6358459-6358478 MS.gene31132:CDS 30.0%
GATTGGGAAACAAAGTTATT+AGG + chr2.4:6357728-6357747 None:intergenic 30.0%
TAACAACAATGTTGAATGTG+AGG - chr2.4:6358828-6358847 MS.gene31132:CDS 30.0%
TAGTTGTATGAGTCTATATG+TGG + chr2.4:6357662-6357681 None:intergenic 30.0%
TATATGTGGATCAAGTTCAA+AGG + chr2.4:6357648-6357667 None:intergenic 30.0%
TATCGTTTATCGAAAAGATC+TGG - chr2.4:6358409-6358428 MS.gene31132:CDS 30.0%
TTTCTCAATGTGTCACAAAA+TGG - chr2.4:6357827-6357846 MS.gene31132:CDS 30.0%
! TTGATAATTCTTCATTCTGC+TGG + chr2.4:6357961-6357980 None:intergenic 30.0%
!! AATGTTCCTTTTGAGTACAA+TGG - chr2.4:6358532-6358551 MS.gene31132:CDS 30.0%
!! CACATTATAGATTTTGGTGT+TGG - chr2.4:6358358-6358377 MS.gene31132:CDS 30.0%
!! GAAAGAGCATTATTGAAAGA+AGG + chr2.4:6357767-6357786 None:intergenic 30.0%
!! TTCTATTTGGTTCTTCTTGT+TGG - chr2.4:6359115-6359134 MS.gene31132:CDS 30.0%
!!! ATAGATTTTGGTGTTGGTTA+TGG - chr2.4:6358364-6358383 MS.gene31132:CDS 30.0%
AAAATTGGATGCTACAAGGT+TGG - chr2.4:6359082-6359101 MS.gene31132:CDS 35.0%
AAGAAGTTATGAGGAAGTTG+TGG - chr2.4:6358006-6358025 MS.gene31132:CDS 35.0%
AAGGAAAATTGGATGCTACA+AGG - chr2.4:6359078-6359097 MS.gene31132:CDS 35.0%
ATGAATGTTATAGCCTGTGA+AGG - chr2.4:6358898-6358917 MS.gene31132:CDS 35.0%
CAAACAGATTCTATGGTTCT+AGG - chr2.4:6357978-6357997 MS.gene31132:CDS 35.0%
CAAAGATTGGCACATTTCTT+TGG - chr2.4:6358145-6358164 MS.gene31132:CDS 35.0%
CATATATGCAATGGCATTCT+AGG - chr2.4:6358947-6358966 MS.gene31132:CDS 35.0%
GAATTCACTTCAACTGTTCT+TGG - chr2.4:6357254-6357273 MS.gene31132:CDS 35.0%
GAGAAGAATGTGTCTTAATC+TGG + chr2.4:6358108-6358127 None:intergenic 35.0%
GAGTATTTGGGAGTAGAAAA+GGG + chr2.4:6357694-6357713 None:intergenic 35.0%
GCAATACCATTGTACTCAAA+AGG + chr2.4:6358541-6358560 None:intergenic 35.0%
GGAAAAAGGTTGAAGTAGAT+TGG + chr2.4:6357745-6357764 None:intergenic 35.0%
GTTTATCGAAAAGATCTGGT+GGG - chr2.4:6358413-6358432 MS.gene31132:CDS 35.0%
TAGGAGACAAAGAAGTTATG+AGG - chr2.4:6357997-6358016 MS.gene31132:CDS 35.0%
TGGAAGGGTAGAATTCTATT+TGG - chr2.4:6359102-6359121 MS.gene31132:CDS 35.0%
TTCACTCTCTCCAATGAATT+TGG + chr2.4:6358472-6358491 None:intergenic 35.0%
! GTGAAGACTTTCTGTTTCTT+TGG + chr2.4:6358341-6358360 None:intergenic 35.0%
! GTTGATATAACCAAGAGCAT+TGG + chr2.4:6357363-6357382 None:intergenic 35.0%
! TGTTTGAGGTGAAGGAAAAT+TGG - chr2.4:6359067-6359086 MS.gene31132:CDS 35.0%
!! GAGAAAATGGAGGAGTATTT+GGG + chr2.4:6357706-6357725 None:intergenic 35.0%
!! TGATTTTCTGTTTGAGGTGA+AGG - chr2.4:6359059-6359078 MS.gene31132:CDS 35.0%
!! TGGGTTTAGATCTTTGAAAC+TGG - chr2.4:6358981-6359000 MS.gene31132:CDS 35.0%
!! TTGGGAAAGTATCAAAGTTC+AGG - chr2.4:6358567-6358586 MS.gene31132:CDS 35.0%
!!! AGTTTTGTTGATACTCATGC+TGG + chr2.4:6357565-6357584 None:intergenic 35.0%
!!! ATGCACCTTTTTTTGTGACA+AGG - chr2.4:6358758-6358777 MS.gene31132:CDS 35.0%
AAATCCAAAACAGCACCTCT+CGG + chr2.4:6358682-6358701 None:intergenic 40.0%
AAGACACATTCTTCTCCAAC+AGG - chr2.4:6358112-6358131 MS.gene31132:CDS 40.0%
ACCTGATGAGACAGTTGTTT+CGG - chr2.4:6358651-6358670 MS.gene31132:CDS 40.0%
ACTCTTCATGCTCATTGTGT+TGG + chr2.4:6357337-6357356 None:intergenic 40.0%
ATGAGGAAGTTGTGGATTTG+AGG - chr2.4:6358014-6358033 MS.gene31132:CDS 40.0%
ATGTGGATCAAGTTCAAAGG+AGG + chr2.4:6357645-6357664 None:intergenic 40.0%
ATGTTTGAGGAAGACGTTTG+GGG - chr2.4:6358865-6358884 MS.gene31132:CDS 40.0%
ATTCTAGGCACATGGGAAAT+GGG - chr2.4:6358962-6358981 MS.gene31132:CDS 40.0%
CGTTTATCGAAAAGATCTGG+TGG - chr2.4:6358412-6358431 MS.gene31132:CDS 40.0%
CTCTTACTTTGTTCATGGAG+TGG + chr2.4:6357233-6357252 None:intergenic 40.0%
GAAAAAATCTGCCGCAGATA+TGG - chr2.4:6358231-6358250 MS.gene31132:CDS 40.0%
GAGTGAATGAAACAGGTCTT+AGG - chr2.4:6358485-6358504 MS.gene31132:CDS 40.0%
GATGTTTGAGGAAGACGTTT+GGG - chr2.4:6358864-6358883 MS.gene31132:CDS 40.0%
GGCATGCTGATGAATAAACT+TGG + chr2.4:6358267-6358286 None:intergenic 40.0%
TCCTCAAACATCAACCTAAC+AGG + chr2.4:6358856-6358875 None:intergenic 40.0%
TGATGTTTGAGGAAGACGTT+TGG - chr2.4:6358863-6358882 MS.gene31132:CDS 40.0%
TGCCATTGCATATATGTCTC+TGG + chr2.4:6358943-6358962 None:intergenic 40.0%
TTGAATGTGAGGATCCTGTT+AGG - chr2.4:6358839-6358858 MS.gene31132:CDS 40.0%
TTGGAGAGAGTGAATGAAAC+AGG - chr2.4:6358478-6358497 MS.gene31132:CDS 40.0%
TTGTTGATACTCATGCTGGA+TGG + chr2.4:6357561-6357580 None:intergenic 40.0%
! CTCAGTAAGTTGAAGAGCAT+AGG + chr2.4:6357438-6357457 None:intergenic 40.0%
! GGAGTATTTGGGAGTAGAAA+AGG + chr2.4:6357695-6357714 None:intergenic 40.0%
! GTAAGCTTTGACCATATCTG+CGG + chr2.4:6358245-6358264 None:intergenic 40.0%
! GTTGAAGAGCATAGGAATCT+TGG + chr2.4:6357430-6357449 None:intergenic 40.0%
!! AGGTGATGGAACTCAAAGAT+TGG - chr2.4:6358132-6358151 MS.gene31132:CDS 40.0%
!! GGAGAAAATGGAGGAGTATT+TGG + chr2.4:6357707-6357726 None:intergenic 40.0%
!! GTGTTGGTTATGGTTTCAAG+TGG - chr2.4:6358374-6358393 MS.gene31132:CDS 40.0%
!! TCCTGTTAGGTTGATGTTTG+AGG - chr2.4:6358852-6358871 MS.gene31132:CDS 40.0%
!!! ATCTGCGGCAGATTTTTTCT+TGG + chr2.4:6358230-6358249 None:intergenic 40.0%
!!! TGGAAGCTTGTGTTTTGAAG+TGG + chr2.4:6357941-6357960 None:intergenic 40.0%
ATCCCTGTGATCCTCAACTT+GGG + chr2.4:6358442-6358461 None:intergenic 45.0%
CAATGGCATTCTAGGCACAT+GGG - chr2.4:6358955-6358974 MS.gene31132:CDS 45.0%
CATTCTAGGCACATGGGAAA+TGG - chr2.4:6358961-6358980 MS.gene31132:CDS 45.0%
CATTCTTCTCCAACAGGTGA+TGG - chr2.4:6358118-6358137 MS.gene31132:CDS 45.0%
GAAGAGTCATCCAATGCTCT+TGG - chr2.4:6357350-6357369 MS.gene31132:CDS 45.0%
GGCCAGAGACATATATGCAA+TGG - chr2.4:6358938-6358957 MS.gene31132:CDS 45.0%
TACTCATGCTGGATGGAAGA+AGG + chr2.4:6357554-6357573 None:intergenic 45.0%
TATCCCTGTGATCCTCAACT+TGG + chr2.4:6358443-6358462 None:intergenic 45.0%
TCCGAAACAACTGTCTCATC+AGG + chr2.4:6358655-6358674 None:intergenic 45.0%
TGGATGCTACAAGGTTGGAA+GGG - chr2.4:6359087-6359106 MS.gene31132:CDS 45.0%
TGTTTGAGGAAGACGTTTGG+GGG - chr2.4:6358866-6358885 MS.gene31132:CDS 45.0%
TTGGATGCTACAAGGTTGGA+AGG - chr2.4:6359086-6359105 MS.gene31132:CDS 45.0%
! CTTTGAGTTCCATCACCTGT+TGG + chr2.4:6358130-6358149 None:intergenic 45.0%
! GGTTATGGTTTCAAGTGGCT+TGG - chr2.4:6358379-6358398 MS.gene31132:CDS 45.0%
AACCCTATCACAGCCTTCAC+AGG + chr2.4:6358914-6358933 None:intergenic 50.0%
AAGGCTGTGATAGGGTTCAG+AGG - chr2.4:6358917-6358936 MS.gene31132:CDS 50.0%
AGCCTGTGAAGGCTGTGATA+GGG - chr2.4:6358909-6358928 MS.gene31132:CDS 50.0%
GAAGCACGTTTAGCAGGAAC+TGG - chr2.4:6358178-6358197 MS.gene31132:CDS 50.0%
GCAATGGCATTCTAGGCACA+TGG - chr2.4:6358954-6358973 MS.gene31132:CDS 50.0%
GCACTTGAAGCACGTTTAGC+AGG - chr2.4:6358172-6358191 MS.gene31132:CDS 50.0%
GTAGGAGGAAGAGGATGAAG+AGG + chr2.4:6357491-6357510 None:intergenic 50.0%
TAGCCTGTGAAGGCTGTGAT+AGG - chr2.4:6358908-6358927 MS.gene31132:CDS 50.0%
! CACCCAAGTTGAGGATCACA+GGG - chr2.4:6358437-6358456 MS.gene31132:CDS 50.0%
! GTTGTTTCGGAGAATCCGAG+AGG - chr2.4:6358664-6358683 MS.gene31132:CDS 50.0%
!!! GAATCCGAGAGGTGCTGTTT+TGG - chr2.4:6358675-6358694 MS.gene31132:CDS 50.0%
CCTGTGATCCTCAACTTGGG+TGG + chr2.4:6358439-6358458 None:intergenic 55.0%
! CCACCCAAGTTGAGGATCAC+AGG - chr2.4:6358436-6358455 MS.gene31132:CDS 55.0%
ACAGCAGCAGCAGCAGCAGT+AGG + chr2.4:6357509-6357528 None:intergenic 60.0%
GCAGCAGCAGTAGGAGGAAG+AGG + chr2.4:6357500-6357519 None:intergenic 60.0%
GCAGCAGCAGCAGCAGTAGG+AGG + chr2.4:6357506-6357525 None:intergenic 65.0%
! CTGGTGGGCCACCCAAGTTG+AGG - chr2.4:6358428-6358447 MS.gene31132:CDS 65.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 6357209 6359149 6357209 ID=MS.gene31132
chr2.4 mRNA 6357209 6359149 6357209 ID=MS.gene31132.t1;Parent=MS.gene31132
chr2.4 exon 6357209 6359149 6357209 ID=MS.gene31132.t1.exon1;Parent=MS.gene31132.t1
chr2.4 CDS 6357209 6359149 6357209 ID=cds.MS.gene31132.t1;Parent=MS.gene31132.t1
Gene Sequence

>MS.gene31132

ATGGACAAAGGAGTTGTTGATCCACTCCATGAACAAAGTAAGAGTGAATTCACTTCAACTGTTCTTGGTTTTATAAACAAATTTCTCATGGAAGATGATGACATAGAAGAAGACTACAATATGTTCCAACACAATGAGCATGAAGAGTCATCCAATGCTCTTGGTTATATCAACCAATTTCTCATGGAAGAAGATTTTGAAAAAGAATACACTATGTTCCAAGATTCCTATGCTCTTCAACTTACTGAGAAATCATTTCATGATGTTATTGCTCACACTCCTCTTCATCCTCTTCCTCCTACTGCTGCTGCTGCTGCTGTTACTGCTTTTACTACTACTCTTCCTTCTTCCATCCAGCATGAGTATCAACAAAACTACAACTTTGTGCATAGCCCTAACTTCAGTGATTATAGTTTAAGCAGTGATAGCACTACCTCCTTTGAACTTGATCCACATATAGACTCATACAACTACAATTATAACCCTTTTCTACTCCCAAATACTCCTCCATTTTCTCCTAATAACTTTGTTTCCCAATCTACTTCAACCTTTTTCCCTTCTTTCAATAATGCTCTTTCTCATGAGCTACTTCAGACAGAAAACTTTGAAGAAGAGCATTTTCTCAATGTGTCACAAAATGGATCAGAACAAGTGTATGTAGATGATAGCAATGAGTTATCAGAATTATTTGATAAAGTACTAGTTCTTGGTACTAAATTCACAAAAGGGCCACTTCAAAACACAAGCTTCCAGCAGAATGAAGAATTATCAAACAGATTCTATGGTTCTAGGAGACAAAGAAGTTATGAGGAAGTTGTGGATTTGAGGACACTATTGATGCTGTGTGCACAATCCATTTCTTGTAATGATATTTCAAATGCTAACCAGTTATTAAACCAGATTAAGACACATTCTTCTCCAACAGGTGATGGAACTCAAAGATTGGCACATTTCTTTGGAAATGCACTTGAAGCACGTTTAGCAGGAACTGGTTCACATATCTATAGAGCTCTATCTTCCAAGAAAAAATCTGCCGCAGATATGGTCAAAGCTTACCAAGTTTATTCATCAGCATGCCCTTTTGAAAAACTTGCAATTATGTTCTCAAACGATGCAATTTTGAATGTAGCCAAAGAAACAGAAAGTCTTCACATTATAGATTTTGGTGTTGGTTATGGTTTCAAGTGGCTTGGTTTTATATATCGTTTATCGAAAAGATCTGGTGGGCCACCCAAGTTGAGGATCACAGGGATAGATCTTCCAAATTCATTGGAGAGAGTGAATGAAACAGGTCTTAGGCTTGCAAGTTATTGCAAACGTTTCAATGTTCCTTTTGAGTACAATGGTATTGCAAAAAATTGGGAAAGTATCAAAGTTCAGGATTTTAAGATTAGGAAAAATGAGTTTGTTGCTGTGACTTGTGTGTTTAAGTTTGAGAATCTACCTGATGAGACAGTTGTTTCGGAGAATCCGAGAGGTGCTGTTTTGGATTTGATTAAGAAGGCAAATCCAAATATATTTATTCATAGCATTGTTAATGGTGGTTATGATGCACCTTTTTTTGTGACAAGGTTTAAGGAAGCTGTTTTTTATTATTCAGCATTGTTTGATATGCTTGATAACAACAATGTTGAATGTGAGGATCCTGTTAGGTTGATGTTTGAGGAAGACGTTTGGGGGAAAGATATAATGAATGTTATAGCCTGTGAAGGCTGTGATAGGGTTCAGAGGCCAGAGACATATATGCAATGGCATTCTAGGCACATGGGAAATGGGTTTAGATCTTTGAAACTGGATAAGCAGATTATAAATAAATTGAAGGGTAAGTTAAGAGATGATGCATATAATAGTGATTTTCTGTTTGAGGTGAAGGAAAATTGGATGCTACAAGGTTGGAAGGGTAGAATTCTATTTGGTTCTTCTTGTTGGATTCCAGCATAG
Protein sequence

>MS.gene31132.t1

MDKGVVDPLHEQSKSEFTSTVLGFINKFLMEDDDIEEDYNMFQHNEHEESSNALGYINQFLMEEDFEKEYTMFQDSYALQLTEKSFHDVIAHTPLHPLPPTAAAAAVTAFTTTLPSSIQHEYQQNYNFVHSPNFSDYSLSSDSTTSFELDPHIDSYNYNYNPFLLPNTPPFSPNNFVSQSTSTFFPSFNNALSHELLQTENFEEEHFLNVSQNGSEQVYVDDSNELSELFDKVLVLGTKFTKGPLQNTSFQQNEELSNRFYGSRRQRSYEEVVDLRTLLMLCAQSISCNDISNANQLLNQIKTHSSPTGDGTQRLAHFFGNALEARLAGTGSHIYRALSSKKKSAADMVKAYQVYSSACPFEKLAIMFSNDAILNVAKETESLHIIDFGVGYGFKWLGFIYRLSKRSGGPPKLRITGIDLPNSLERVNETGLRLASYCKRFNVPFEYNGIAKNWESIKVQDFKIRKNEFVAVTCVFKFENLPDETVVSENPRGAVLDLIKKANPNIFIHSIVNGGYDAPFFVTRFKEAVFYYSALFDMLDNNNVECEDPVRLMFEEDVWGKDIMNVIACEGCDRVQRPETYMQWHSRHMGNGFRSLKLDKQIINKLKGKLRDDAYNSDFLFEVKENWMLQGWKGRILFGSSCWIPA