AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene31133

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31133.t1 MTR_2g097350 91.769 571 46 1 1 570 72 642 0.0 1036
MS.gene31133.t1 MTR_2g097390 83.218 578 81 4 2 570 73 643 0.0 950
MS.gene31133.t1 MTR_2g097310 82.842 577 81 5 1 570 75 640 0.0 939
MS.gene31133.t1 MTR_2g097380 75.556 495 70 5 83 570 113 563 0.0 755
MS.gene31133.t1 MTR_4g064160 61.036 444 160 6 137 570 246 686 0.0 558
MS.gene31133.t1 MTR_2g097410 51.678 447 198 9 137 570 302 743 1.30e-155 463
MS.gene31133.t1 MTR_7g062120 52.455 387 178 3 188 570 358 742 3.10e-152 455
MS.gene31133.t1 MTR_4g064120 42.000 600 293 13 2 570 53 628 3.98e-152 451
MS.gene31133.t1 MTR_4g064150 41.812 629 301 16 2 570 112 735 4.25e-149 446
MS.gene31133.t1 MTR_4g064180 40.962 603 295 13 2 570 53 628 2.27e-147 439
MS.gene31133.t1 MTR_4g064200 47.072 461 217 7 130 570 199 652 6.05e-144 431
MS.gene31133.t1 MTR_2g097467 38.230 599 324 13 1 570 75 656 1.45e-139 419
MS.gene31133.t1 MTR_2g097463 38.538 602 319 12 1 570 75 657 1.35e-138 417
MS.gene31133.t1 MTR_2g097473 38.499 613 303 14 1 570 75 656 1.29e-136 412
MS.gene31133.t1 MTR_3g056110 28.962 549 330 16 51 567 21 541 4.48e-64 219
MS.gene31133.t1 MTR_5g097480 32.267 375 245 4 198 567 173 543 1.37e-63 218
MS.gene31133.t1 MTR_3g089055 33.957 374 236 6 200 567 196 564 7.70e-62 214
MS.gene31133.t1 MTR_3g089055 33.957 374 236 6 200 567 201 569 8.00e-62 214
MS.gene31133.t1 MTR_2g026250 32.376 383 248 5 193 567 216 595 2.50e-61 213
MS.gene31133.t1 MTR_4g133660 31.034 377 252 4 197 568 170 543 3.19e-60 209
MS.gene31133.t1 MTR_2g082090 32.000 375 246 4 198 567 208 578 6.57e-60 209
MS.gene31133.t1 MTR_1g029420 32.527 372 244 3 200 567 223 591 3.51e-59 207
MS.gene31133.t1 MTR_5g094450 31.234 381 245 9 198 567 157 531 1.17e-56 199
MS.gene31133.t1 MTR_7g027190 30.867 392 236 7 197 567 295 672 1.93e-54 196
MS.gene31133.t1 MTR_4g104020 29.058 382 250 8 199 567 146 519 2.33e-49 179
MS.gene31133.t1 MTR_8g442410 30.670 388 239 11 197 567 150 524 3.90e-49 179
MS.gene31133.t1 MTR_3g065980 31.122 392 240 7 192 567 162 539 2.25e-48 177
MS.gene31133.t1 MTR_7g074650 32.055 365 234 8 206 569 442 793 4.39e-48 179
MS.gene31133.t1 MTR_5g009080 26.818 440 258 8 188 567 42 477 7.87e-43 160
MS.gene31133.t1 MTR_3g022830 27.685 419 240 9 203 567 26 435 2.35e-40 152
MS.gene31133.t1 MTR_4g076140 26.087 437 258 9 191 567 37 468 5.16e-40 152
MS.gene31133.t1 MTR_4g102790 25.648 386 263 9 198 569 288 663 8.29e-40 154
MS.gene31133.t1 MTR_4g097080 26.509 381 262 8 200 569 131 504 4.15e-39 150
MS.gene31133.t1 MTR_4g077760 29.211 380 242 10 199 567 191 554 1.89e-37 146
MS.gene31133.t1 MTR_2g034260 25.641 390 266 8 194 567 202 583 1.89e-36 144
MS.gene31133.t1 MTR_2g089100 28.537 410 242 10 199 570 49 445 5.47e-36 140
MS.gene31133.t1 MTR_2g034250 26.667 390 263 8 194 567 202 584 4.35e-34 137
MS.gene31133.t1 MTR_5g015490 26.562 384 260 10 202 569 114 491 3.45e-33 133
MS.gene31133.t1 MTR_2g034280 25.389 386 270 7 194 567 195 574 3.16e-32 131
MS.gene31133.t1 MTR_7g057230 28.571 287 194 5 287 567 375 656 3.94e-31 128
MS.gene31133.t1 MTR_4g076020 27.346 373 234 10 206 570 88 431 5.17e-31 125
MS.gene31133.t1 MTR_1g069725 28.084 381 248 14 194 567 101 462 1.43e-30 125
MS.gene31133.t1 MTR_6g047750 27.895 380 245 11 203 567 296 661 5.60e-30 125
MS.gene31133.t1 MTR_1g096030 27.411 394 243 14 202 567 80 458 1.66e-29 122
MS.gene31133.t1 MTR_7g109580 26.440 382 258 10 197 567 184 553 2.49e-28 119
MS.gene31133.t1 MTR_5g058860 27.296 392 245 16 202 567 119 496 1.45e-25 110
MS.gene31133.t1 MTR_2g099110 28.462 390 246 11 202 569 74 452 1.54e-25 110
MS.gene31133.t1 MTR_3g072710 26.368 402 248 14 197 567 115 499 9.20e-24 105
MS.gene31133.t1 MTR_4g095500 22.877 424 266 10 199 570 56 470 3.18e-23 103
MS.gene31133.t1 MTR_5g015950 22.066 426 269 11 199 570 32 448 2.48e-22 100
MS.gene31133.t1 MTR_3g053270 22.066 426 269 11 199 570 32 448 2.48e-22 100
MS.gene31133.t1 MTR_1g086970 24.936 393 257 15 197 567 101 477 7.59e-22 99.4
MS.gene31133.t1 MTR_8g093070 24.051 395 273 13 185 570 122 498 3.23e-19 91.3
MS.gene31133.t1 MTR_0092s0100 23.885 381 248 14 203 567 373 727 6.46e-15 78.6
MS.gene31133.t1 MTR_0092s0100 23.885 381 248 14 203 567 376 730 6.71e-15 78.6
MS.gene31133.t1 MTR_4g026485 25.632 277 180 10 203 468 270 531 8.08e-14 74.7
MS.gene31133.t1 MTR_8g020840 24.372 398 248 12 202 567 171 547 3.71e-12 69.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31133.t1 AT1G07530 51.627 461 203 7 127 570 310 767 4.28e-166 491
MS.gene31133.t1 AT2G29060 43.095 601 287 11 1 569 79 656 1.15e-164 484
MS.gene31133.t1 AT2G29060 42.139 617 307 11 1 569 79 693 5.79e-163 481
MS.gene31133.t1 AT1G07520 48.098 447 216 8 135 570 90 531 2.25e-145 430
MS.gene31133.t1 AT1G07520 48.098 447 216 8 135 570 90 531 2.25e-145 430
MS.gene31133.t1 AT1G07520 48.098 447 216 8 135 570 254 695 5.57e-144 432
MS.gene31133.t1 AT1G07520 48.098 447 216 8 135 570 255 696 6.05e-144 432
MS.gene31133.t1 AT2G29065 41.695 590 308 12 1 570 57 630 1.64e-141 424
MS.gene31133.t1 AT2G37650 50.260 384 183 4 190 569 323 702 1.47e-133 405
MS.gene31133.t1 AT2G37650 50.260 384 183 4 190 569 335 714 1.75e-133 406
MS.gene31133.t1 AT5G59450 37.197 578 296 10 3 570 80 600 1.01e-121 372
MS.gene31133.t1 AT3G46600 43.897 426 228 6 149 570 131 549 1.80e-120 367
MS.gene31133.t1 AT3G46600 43.897 426 228 6 149 570 163 581 3.47e-120 367
MS.gene31133.t1 AT3G46600 46.011 376 196 4 199 570 1 373 3.95e-116 349
MS.gene31133.t1 AT1G50600 33.835 399 250 6 175 567 135 525 3.84e-66 224
MS.gene31133.t1 AT1G50600 33.835 399 250 6 175 567 210 600 9.73e-66 225
MS.gene31133.t1 AT1G50600 33.835 399 250 6 175 567 206 596 1.05e-65 225
MS.gene31133.t1 AT5G48150 33.418 392 253 5 181 567 101 489 1.12e-65 222
MS.gene31133.t1 AT5G48150 33.418 392 253 5 181 567 101 489 1.12e-65 222
MS.gene31133.t1 AT5G48150 33.418 392 253 5 181 567 101 489 1.12e-65 222
MS.gene31133.t1 AT5G48150 33.418 392 253 5 181 567 101 489 1.12e-65 222
MS.gene31133.t1 AT2G04890 32.345 371 243 5 198 567 49 412 3.35e-64 216
MS.gene31133.t1 AT1G21450 28.625 538 330 14 58 567 81 592 7.44e-58 204
MS.gene31133.t1 AT4G17230 31.481 378 250 6 197 569 153 526 9.50e-54 191
MS.gene31133.t1 AT4G17230 31.481 378 250 6 197 569 153 526 9.50e-54 191
MS.gene31133.t1 AT3G54220 33.333 396 247 9 178 569 269 651 1.03e-52 191
MS.gene31133.t1 AT3G03450 29.793 386 247 9 192 567 173 544 8.98e-49 178
MS.gene31133.t1 AT1G66350 30.871 379 241 8 192 567 145 505 5.74e-48 175
MS.gene31133.t1 AT2G01570 30.052 386 241 9 192 567 214 580 3.16e-46 171
MS.gene31133.t1 AT5G17490 28.117 377 255 7 194 567 152 515 7.33e-45 166
MS.gene31133.t1 AT1G14920 28.796 382 251 7 192 567 162 528 2.44e-44 165
MS.gene31133.t1 AT5G66770 30.541 370 239 9 206 567 224 583 7.97e-40 153
MS.gene31133.t1 AT1G50420 24.161 447 275 8 181 567 36 478 5.17e-38 146
MS.gene31133.t1 AT4G37650 26.331 357 235 8 201 535 145 495 5.71e-34 135
MS.gene31133.t1 AT3G50650 27.451 357 238 7 222 567 195 541 8.83e-34 135
MS.gene31133.t1 AT1G63100 26.776 366 253 7 190 550 269 624 9.81e-33 133
MS.gene31133.t1 AT1G63100 27.027 370 247 8 190 550 244 599 1.01e-32 133
MS.gene31133.t1 AT5G41920 29.474 380 243 12 196 567 37 399 1.91e-32 129
MS.gene31133.t1 AT5G52510 26.992 389 248 12 200 567 266 639 2.89e-32 132
MS.gene31133.t1 AT1G55580 27.163 416 244 11 199 567 41 444 1.78e-29 121
MS.gene31133.t1 AT3G49950 25.000 392 264 9 199 569 27 409 2.75e-27 114
MS.gene31133.t1 AT4G08250 25.575 391 263 13 194 567 97 476 5.06e-25 108
MS.gene31133.t1 AT3G13840 24.804 383 250 12 202 567 148 509 1.71e-14 76.6

Find 98 sgRNAs with CRISPR-Local

Find 130 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCACTCTCTCCAACAAATT+TGG 0.135021 2.4:+6363325 None:intergenic
AGTCTTCACATTATAGATTT+TGG 0.151926 2.4:-6363442 MS.gene31133:CDS
GGAGGAATTGGAAGAGTATT+TGG 0.153984 2.4:+6364069 None:intergenic
ATGCTATGAACAAATATATT+TGG 0.177812 2.4:+6363076 None:intergenic
CAAAGATTGGCACATTTCTT+TGG 0.204755 2.4:-6363649 MS.gene31133:CDS
TGGGTTTAAGTCTTTGAAAT+TGG 0.243482 2.4:-6362813 MS.gene31133:CDS
AAATCCGAGGGGTGCTGTTT+TGG 0.247098 2.4:-6363119 MS.gene31133:CDS
TATCCCTGTGATCCTCAATT+TGG 0.247519 2.4:+6363354 None:intergenic
TCTTAATCTGGTTTAATAAC+TGG 0.258792 2.4:+6363701 None:intergenic
GAAACAAAGTTTGGAGGAAT+TGG 0.270095 2.4:+6364057 None:intergenic
TTCTATTTGGTTCTTCTTGT+TGG 0.274191 2.4:-6362679 MS.gene31133:CDS
TGAAATATCATTACAAGAAA+TGG 0.276552 2.4:+6363732 None:intergenic
TTTGTTCATAGCATTGTTAA+TGG 0.283287 2.4:-6363067 MS.gene31133:CDS
ATCTGCAGCAGATTTCTTCT+TGG 0.288366 2.4:+6363567 None:intergenic
TAGTTGTATGAGTCTATAAT+TGG 0.294656 2.4:+6364114 None:intergenic
CAAACAGATTCTATGGTTAT+AGG 0.296898 2.4:-6363816 MS.gene31133:CDS
AGTAATTTATCAAATAATTC+TGG 0.301090 2.4:+6363907 None:intergenic
GAGAAGAATGTGTCTTAATC+TGG 0.320149 2.4:+6363689 None:intergenic
GAGGAATTGGAAGAGTATTT+GGG 0.326156 2.4:+6364070 None:intergenic
TGGAAGGGTAGAGTTCTATT+TGG 0.329745 2.4:-6362692 MS.gene31133:CDS
TCGAATTGAGAAACAAAGTT+TGG 0.339338 2.4:+6364048 None:intergenic
GAATTATCAAACAGATTCTA+TGG 0.344805 2.4:-6363823 MS.gene31133:CDS
TCCTCAAACATCAACCTATT+AGG 0.349519 2.4:+6362941 None:intergenic
TGATGTTTGAGGAAGAGTTT+TGG 0.352833 2.4:-6362931 MS.gene31133:CDS
GCAACTTATTGCGAACGTTT+CGG 0.353167 2.4:-6363283 MS.gene31133:CDS
GAAGAAATCTGCTGCAGATA+TGG 0.361247 2.4:-6363563 MS.gene31133:CDS
AGAGTATTTGGGAGTGGAAA+AGG 0.382981 2.4:+6364081 None:intergenic
ATCCCTGTGATCCTCAATTT+GGG 0.383992 2.4:+6363355 None:intergenic
ACCTGATGAGACAGTTGTTT+CGG 0.385460 2.4:-6363143 MS.gene31133:CDS
GCAATACCATTGAACTCAAA+AGG 0.401581 2.4:+6363256 None:intergenic
GTTGAAGAGCAAAGGAATCT+TGG 0.406232 2.4:+6364352 None:intergenic
GAGTGAATGGAACAGGTCTT+AGG 0.408191 2.4:-6363309 MS.gene31133:CDS
GATAGATCTTCCAAATTTGT+TGG 0.422261 2.4:-6363335 MS.gene31133:CDS
AAACTATAATCACTGATGTC+AGG 0.427646 2.4:+6364171 None:intergenic
TTGAGAGGCCAGAGACATAT+AGG 0.428001 2.4:-6362862 MS.gene31133:CDS
TTGAACGTGAGGATCCTAAT+AGG 0.434511 2.4:-6362955 MS.gene31133:CDS
TTTGATAAATTACTAGTCCT+TGG 0.440112 2.4:-6363898 MS.gene31133:CDS
ATGAGGAAGTTGTGGATTTG+AGG 0.441434 2.4:-6363780 MS.gene31133:CDS
TAGCTTGTGAAGGCTGTGAT+AGG 0.447026 2.4:-6362886 MS.gene31133:CDS
TTGGAGAGAGTGAATGGAAC+AGG 0.449006 2.4:-6363316 MS.gene31133:CDS
AAGACACATTCTTCTCCAAC+AGG 0.450645 2.4:-6363682 MS.gene31133:CDS
TCCGAAACAACTGTCTCATC+AGG 0.452006 2.4:+6363142 None:intergenic
GAGAAAATGCTCTTCTTCAA+AGG 0.453527 2.4:+6363984 None:intergenic
GAAGCACGTTTAGCAGGAAC+TGG 0.455780 2.4:-6363616 MS.gene31133:CDS
CTTTGAGTTCCATCACCTGT+TGG 0.456885 2.4:+6363667 None:intergenic
TGCCATTGCCTATATGTCTC+TGG 0.465075 2.4:+6362854 None:intergenic
GAAGTGGAGGAAGGAGGAGG+AGG 0.469076 2.4:+6364252 None:intergenic
GGTTATGGTTTCAAGTGGCC+TGG 0.469133 2.4:-6363415 MS.gene31133:CDS
AATTTGTTGGAGAGAGTGAA+TGG 0.471019 2.4:-6363322 MS.gene31133:CDS
CATCGTTTATCGAAAAGATC+TGG 0.473353 2.4:-6363385 MS.gene31133:CDS
TCCTAATAGGTTGATGTTTG+AGG 0.474185 2.4:-6362942 MS.gene31133:CDS
GAGTATTTGGGAGTGGAAAA+GGG 0.483840 2.4:+6364082 None:intergenic
GAAGAAGTGGAGGAAGGAGG+AGG 0.488333 2.4:+6364249 None:intergenic
GCACTTGAAGCACGTTTAGC+AGG 0.499951 2.4:-6363622 MS.gene31133:CDS
CATGTTGTGAAGCTCTATGC+TGG 0.501576 2.4:+6362653 None:intergenic
CCACCCAAATTGAGGATCAC+AGG 0.506955 2.4:-6363358 MS.gene31133:CDS
CTCAGTAAGTTGAAGAGCAA+AGG 0.507074 2.4:+6364344 None:intergenic
TAGGAGACAAAGAAGTTATG+AGG 0.508963 2.4:-6363797 MS.gene31133:CDS
AAAATTGGATGCTACAAGGT+TGG 0.509330 2.4:-6362712 MS.gene31133:CDS
GCAGTAGCAGTAGGAGGAGG+AGG 0.511002 2.4:+6364282 None:intergenic
CATTCTTCTCCAACAGGTGA+TGG 0.515669 2.4:-6363676 MS.gene31133:CDS
ATTGGAAGAGTATTTGGGAG+TGG 0.520042 2.4:+6364075 None:intergenic
AATTGAGAAACAAAGTTTGG+AGG 0.522706 2.4:+6364051 None:intergenic
AAGAAGTTATGAGGAAGTTG+TGG 0.533441 2.4:-6363788 MS.gene31133:CDS
AGAAGAAGACGACGAAGAAG+TGG 0.533488 2.4:+6364236 None:intergenic
GCATGTTATAGATAAATTGA+AGG 0.536806 2.4:-6362786 MS.gene31133:CDS
CATGTTATAGATAAATTGAA+GGG 0.543479 2.4:-6362785 MS.gene31133:CDS
GTCTTAATTACCTCATGAGA+AGG 0.545201 2.4:+6364012 None:intergenic
GGCATGCTGATGAATAAACT+TGG 0.547453 2.4:+6363530 None:intergenic
TTGGATGCTACAAGGTTGGA+AGG 0.548278 2.4:-6362708 MS.gene31133:CDS
TTGGGAAAGTATCAAAGTTG+AGG 0.550259 2.4:-6363227 MS.gene31133:CDS
CTGGCGGGCCACCCAAATTG+AGG 0.551191 2.4:-6363366 MS.gene31133:CDS
GTAGCAGTAGGAGGAGGAGG+AGG 0.552690 2.4:+6364285 None:intergenic
AACTATAATCACTGATGTCA+GGG 0.555622 2.4:+6364172 None:intergenic
AGGTGATGGAACTCAAAGAT+TGG 0.559955 2.4:-6363662 MS.gene31133:CDS
GCAGCAGTAGCAGTAGGAGG+AGG 0.561458 2.4:+6364279 None:intergenic
GGAGCAGCAGCAGTAGCAGT+AGG 0.562763 2.4:+6364273 None:intergenic
TATAATTGGATCAAGTTCAA+AGG 0.570239 2.4:+6364128 None:intergenic
GGCCAGAGACATATAGGCAA+TGG 0.582352 2.4:-6362856 MS.gene31133:CDS
CAGTTGTTTCGGAAAATCCG+AGG 0.585137 2.4:-6363132 MS.gene31133:CDS
GACGACGAAGAAGTGGAGGA+AGG 0.594630 2.4:+6364243 None:intergenic
TCTTAATTACCTCATGAGAA+GGG 0.597606 2.4:+6364013 None:intergenic
AGTTGTTTCGGAAAATCCGA+GGG 0.598269 2.4:-6363131 MS.gene31133:CDS
CACCCAAATTGAGGATCACA+GGG 0.601284 2.4:-6363357 MS.gene31133:CDS
AGAAGACGACGAAGAAGTGG+AGG 0.605523 2.4:+6364239 None:intergenic
GTGTTGGTTATGGTTTCAAG+TGG 0.612035 2.4:-6363420 MS.gene31133:CDS
GACGAAGAAGTGGAGGAAGG+AGG 0.614142 2.4:+6364246 None:intergenic
AAATCCAAAACAGCACCCCT+CGG 0.616918 2.4:+6363115 None:intergenic
GCAGCAGCAGTAGCAGTAGG+AGG 0.617587 2.4:+6364276 None:intergenic
CCTGTGATCCTCAATTTGGG+TGG 0.623258 2.4:+6363358 None:intergenic
GATAAACGATGTATTAAACC+AGG 0.631290 2.4:+6363397 None:intergenic
GTTTATCGAAAAGATCTGGC+GGG 0.631371 2.4:-6363381 MS.gene31133:CDS
TGGATGCTACAAGGTTGGAA+GGG 0.641743 2.4:-6362707 MS.gene31133:CDS
CGTTTATCGAAAAGATCTGG+CGG 0.648801 2.4:-6363382 MS.gene31133:CDS
TAACAACAATGTTGAACGTG+AGG 0.650267 2.4:-6362966 MS.gene31133:CDS
ATGAATGTTATAGCTTGTGA+AGG 0.662961 2.4:-6362896 MS.gene31133:CDS
AAGGCTGTGATAGGATTGAG+AGG 0.704778 2.4:-6362877 MS.gene31133:CDS
GTTGTTTCGGAAAATCCGAG+GGG 0.754460 2.4:-6363130 MS.gene31133:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGTAATTTATCAAATAATTC+TGG + chr2.4:6363120-6363139 None:intergenic 15.0%
!! ATGCTATGAACAAATATATT+TGG + chr2.4:6363951-6363970 None:intergenic 20.0%
!! CATGTTATAGATAAATTGAA+GGG - chr2.4:6364239-6364258 MS.gene31133:CDS 20.0%
!! TGAAATATCATTACAAGAAA+TGG + chr2.4:6363295-6363314 None:intergenic 20.0%
!! TTGGTACTAAAGTAAAAAAA+GGG - chr2.4:6363145-6363164 MS.gene31133:CDS 20.0%
! AATAAAAATTGGATGCTACA+AGG - chr2.4:6364308-6364327 MS.gene31133:CDS 25.0%
! CAATGGTATTGCAAAAAATT+GGG - chr2.4:6363779-6363798 MS.gene31133:CDS 25.0%
! CTTGGTACTAAAGTAAAAAA+AGG - chr2.4:6363144-6363163 MS.gene31133:CDS 25.0%
! GAATTATCAAACAGATTCTA+TGG - chr2.4:6363201-6363220 MS.gene31133:CDS 25.0%
! GCATGTTATAGATAAATTGA+AGG - chr2.4:6364238-6364257 MS.gene31133:CDS 25.0%
! TAGTTGTATGAGTCTATAAT+TGG + chr2.4:6362913-6362932 None:intergenic 25.0%
! TATAATTGGATCAAGTTCAA+AGG + chr2.4:6362899-6362918 None:intergenic 25.0%
! TCAATGGTATTGCAAAAAAT+TGG - chr2.4:6363778-6363797 MS.gene31133:CDS 25.0%
! TTAAACCTTGTCACAAAAAA+AGG + chr2.4:6363996-6364015 None:intergenic 25.0%
! TTTGATAAATTACTAGTCCT+TGG - chr2.4:6363126-6363145 MS.gene31133:CDS 25.0%
! TTTGTTCATAGCATTGTTAA+TGG - chr2.4:6363957-6363976 MS.gene31133:CDS 25.0%
!! AGTCTTCACATTATAGATTT+TGG - chr2.4:6363582-6363601 MS.gene31133:CDS 25.0%
!! ATAATTGCAAGCTTTTCAAA+AGG + chr2.4:6363519-6363538 None:intergenic 25.0%
!! TAATAGTGATTTTCTGTTTG+AGG - chr2.4:6364283-6364302 MS.gene31133:CDS 25.0%
!! TAATTGCAAGCTTTTCAAAA+GGG + chr2.4:6363518-6363537 None:intergenic 25.0%
!! TCTTAATCTGGTTTAATAAC+TGG + chr2.4:6363326-6363345 None:intergenic 25.0%
!! TGTTTGAGGTGAATAAAAAT+TGG - chr2.4:6364297-6364316 MS.gene31133:CDS 25.0%
!!! AAGTTGAGGATTTTAAGATA+AGG - chr2.4:6363811-6363830 MS.gene31133:CDS 25.0%
!!! CTTTTTTTACTTTAGTACCA+AGG + chr2.4:6363146-6363165 None:intergenic 25.0%
!!! TGTTTTGGATTTGATTAAGA+AGG - chr2.4:6363920-6363939 MS.gene31133:CDS 25.0%
!!! TTTTTTTGTGACAAGGTTTA+AGG - chr2.4:6363995-6364014 MS.gene31133:CDS 25.0%
AAACTATAATCACTGATGTC+AGG + chr2.4:6362856-6362875 None:intergenic 30.0%
AACTATAATCACTGATGTCA+GGG + chr2.4:6362855-6362874 None:intergenic 30.0%
AATTGAGAAACAAAGTTTGG+AGG + chr2.4:6362976-6362995 None:intergenic 30.0%
CAAACAGATTCTATGGTTAT+AGG - chr2.4:6363208-6363227 MS.gene31133:CDS 30.0%
GATAAACGATGTATTAAACC+AGG + chr2.4:6363630-6363649 None:intergenic 30.0%
GATAGATCTTCCAAATTTGT+TGG - chr2.4:6363689-6363708 MS.gene31133:CDS 30.0%
TCGAATTGAGAAACAAAGTT+TGG + chr2.4:6362979-6362998 None:intergenic 30.0%
TCTTAATTACCTCATGAGAA+GGG + chr2.4:6363014-6363033 None:intergenic 30.0%
! ATGAATGTTATAGCTTGTGA+AGG - chr2.4:6364128-6364147 MS.gene31133:CDS 30.0%
!! CACATTATAGATTTTGGTGT+TGG - chr2.4:6363588-6363607 MS.gene31133:CDS 30.0%
!! TGGGTTTAAGTCTTTGAAAT+TGG - chr2.4:6364211-6364230 MS.gene31133:CDS 30.0%
!! TTCTATTTGGTTCTTCTTGT+TGG - chr2.4:6364345-6364364 MS.gene31133:CDS 30.0%
!!! ATAGATTTTGGTGTTGGTTA+TGG - chr2.4:6363594-6363613 MS.gene31133:CDS 30.0%
!!! ATTTGTAGTTTTGTTGATGC+TGG + chr2.4:6362822-6362841 None:intergenic 30.0%
AAAATTGGATGCTACAAGGT+TGG - chr2.4:6364312-6364331 MS.gene31133:CDS 35.0%
AAGAAGTTATGAGGAAGTTG+TGG - chr2.4:6363236-6363255 MS.gene31133:CDS 35.0%
CAAAGATTGGCACATTTCTT+TGG - chr2.4:6363375-6363394 MS.gene31133:CDS 35.0%
CATCGTTTATCGAAAAGATC+TGG - chr2.4:6363639-6363658 MS.gene31133:CDS 35.0%
GAAACAAAGTTTGGAGGAAT+TGG + chr2.4:6362970-6362989 None:intergenic 35.0%
GAGAAAATGCTCTTCTTCAA+AGG + chr2.4:6363043-6363062 None:intergenic 35.0%
GAGAAGAATGTGTCTTAATC+TGG + chr2.4:6363338-6363357 None:intergenic 35.0%
GCAATACCATTGAACTCAAA+AGG + chr2.4:6363771-6363790 None:intergenic 35.0%
GTCTTAATTACCTCATGAGA+AGG + chr2.4:6363015-6363034 None:intergenic 35.0%
TAACAACAATGTTGAACGTG+AGG - chr2.4:6364058-6364077 MS.gene31133:CDS 35.0%
TAGGAGACAAAGAAGTTATG+AGG - chr2.4:6363227-6363246 MS.gene31133:CDS 35.0%
TCCTCAAACATCAACCTATT+AGG + chr2.4:6364086-6364105 None:intergenic 35.0%
TTCACTCTCTCCAACAAATT+TGG + chr2.4:6363702-6363721 None:intergenic 35.0%
! ATGTTTGAGGAAGAGTTTTG+GGG - chr2.4:6364095-6364114 MS.gene31133:CDS 35.0%
! ATTTTAGGCACATGGGAAAT+GGG - chr2.4:6364192-6364211 MS.gene31133:CDS 35.0%
! CATATAGGCAATGGCATTTT+AGG - chr2.4:6364177-6364196 MS.gene31133:CDS 35.0%
! GATGTTTGAGGAAGAGTTTT+GGG - chr2.4:6364094-6364113 MS.gene31133:CDS 35.0%
! TGATGTTTGAGGAAGAGTTT+TGG - chr2.4:6364093-6364112 MS.gene31133:CDS 35.0%
!! AATTTGTTGGAGAGAGTGAA+TGG - chr2.4:6363702-6363721 MS.gene31133:CDS 35.0%
!! GAGGAATTGGAAGAGTATTT+GGG + chr2.4:6362957-6362976 None:intergenic 35.0%
!! TCCTAATAGGTTGATGTTTG+AGG - chr2.4:6364082-6364101 MS.gene31133:CDS 35.0%
!! TGAAAGCTTGTGTTTTCAAG+TGG + chr2.4:6363171-6363190 None:intergenic 35.0%
!! TTGGGAAAGTATCAAAGTTG+AGG - chr2.4:6363797-6363816 MS.gene31133:CDS 35.0%
!!! ATGCACCTTTTTTTGTGACA+AGG - chr2.4:6363988-6364007 MS.gene31133:CDS 35.0%
AAGACACATTCTTCTCCAAC+AGG - chr2.4:6363342-6363361 MS.gene31133:CDS 40.0%
ACCTGATGAGACAGTTGTTT+CGG - chr2.4:6363881-6363900 MS.gene31133:CDS 40.0%
ATCCCTGTGATCCTCAATTT+GGG + chr2.4:6363672-6363691 None:intergenic 40.0%
ATCTGCAGCAGATTTCTTCT+TGG + chr2.4:6363460-6363479 None:intergenic 40.0%
ATGAGGAAGTTGTGGATTTG+AGG - chr2.4:6363244-6363263 MS.gene31133:CDS 40.0%
CGTTTATCGAAAAGATCTGG+CGG - chr2.4:6363642-6363661 MS.gene31133:CDS 40.0%
GAAGAAATCTGCTGCAGATA+TGG - chr2.4:6363461-6363480 MS.gene31133:CDS 40.0%
GAGTATTTGGGAGTGGAAAA+GGG + chr2.4:6362945-6362964 None:intergenic 40.0%
GCAACTTATTGCGAACGTTT+CGG - chr2.4:6363741-6363760 MS.gene31133:CDS 40.0%
GGCATGCTGATGAATAAACT+TGG + chr2.4:6363497-6363516 None:intergenic 40.0%
GTTGAAGAGCAAAGGAATCT+TGG + chr2.4:6362675-6362694 None:intergenic 40.0%
GTTTATCGAAAAGATCTGGC+GGG - chr2.4:6363643-6363662 MS.gene31133:CDS 40.0%
TATCCCTGTGATCCTCAATT+TGG + chr2.4:6363673-6363692 None:intergenic 40.0%
TGGAAGGGTAGAGTTCTATT+TGG - chr2.4:6364332-6364351 MS.gene31133:CDS 40.0%
TTGAACGTGAGGATCCTAAT+AGG - chr2.4:6364069-6364088 MS.gene31133:CDS 40.0%
! AACTGTTTTCCCTTCTCATG+AGG - chr2.4:6363002-6363021 MS.gene31133:CDS 40.0%
! AGAGTATTTGGGAGTGGAAA+AGG + chr2.4:6362946-6362965 None:intergenic 40.0%
! AGTTGTTTCGGAAAATCCGA+GGG - chr2.4:6363893-6363912 MS.gene31133:CDS 40.0%
! CAATGGCATTTTAGGCACAT+GGG - chr2.4:6364185-6364204 MS.gene31133:CDS 40.0%
! CATTTTAGGCACATGGGAAA+TGG - chr2.4:6364191-6364210 MS.gene31133:CDS 40.0%
! CTCAGTAAGTTGAAGAGCAA+AGG + chr2.4:6362683-6362702 None:intergenic 40.0%
!! AGGTGATGGAACTCAAAGAT+TGG - chr2.4:6363362-6363381 MS.gene31133:CDS 40.0%
!! ATTGGAAGAGTATTTGGGAG+TGG + chr2.4:6362952-6362971 None:intergenic 40.0%
!! GGAGGAATTGGAAGAGTATT+TGG + chr2.4:6362958-6362977 None:intergenic 40.0%
!! GTGTTGGTTATGGTTTCAAG+TGG - chr2.4:6363604-6363623 MS.gene31133:CDS 40.0%
!! TGTTTGAGGAAGAGTTTTGG+GGG - chr2.4:6364096-6364115 MS.gene31133:CDS 40.0%
!!! GGTGTTCCTTTTGAGTTCAA+TGG - chr2.4:6363762-6363781 MS.gene31133:CDS 40.0%
!!! GTAGTTTTGTTGATGCTGGA+TGG + chr2.4:6362818-6362837 None:intergenic 40.0%
AAATCCAAAACAGCACCCCT+CGG + chr2.4:6363912-6363931 None:intergenic 45.0%
AAGGCTGTGATAGGATTGAG+AGG - chr2.4:6364147-6364166 MS.gene31133:CDS 45.0%
AGAAGAAGACGACGAAGAAG+TGG + chr2.4:6362791-6362810 None:intergenic 45.0%
CACCCAAATTGAGGATCACA+GGG - chr2.4:6363667-6363686 MS.gene31133:CDS 45.0%
CATTCTTCTCCAACAGGTGA+TGG - chr2.4:6363348-6363367 MS.gene31133:CDS 45.0%
GAGTGAATGGAACAGGTCTT+AGG - chr2.4:6363715-6363734 MS.gene31133:CDS 45.0%
TCCGAAACAACTGTCTCATC+AGG + chr2.4:6363885-6363904 None:intergenic 45.0%
TGCCATTGCCTATATGTCTC+TGG + chr2.4:6364173-6364192 None:intergenic 45.0%
TGGATGCTACAAGGTTGGAA+GGG - chr2.4:6364317-6364336 MS.gene31133:CDS 45.0%
TTGAGAGGCCAGAGACATAT+AGG - chr2.4:6364162-6364181 MS.gene31133:CDS 45.0%
TTGGAGAGAGTGAATGGAAC+AGG - chr2.4:6363708-6363727 MS.gene31133:CDS 45.0%
TTGGATGCTACAAGGTTGGA+AGG - chr2.4:6364316-6364335 MS.gene31133:CDS 45.0%
! CAGTTGTTTCGGAAAATCCG+AGG - chr2.4:6363892-6363911 MS.gene31133:CDS 45.0%
! CTTTGAGTTCCATCACCTGT+TGG + chr2.4:6363360-6363379 None:intergenic 45.0%
! GCAATGGCATTTTAGGCACA+TGG - chr2.4:6364184-6364203 MS.gene31133:CDS 45.0%
! GTTGTTTCGGAAAATCCGAG+GGG - chr2.4:6363894-6363913 MS.gene31133:CDS 45.0%
! TAGCTTGTGAAGGCTGTGAT+AGG - chr2.4:6364138-6364157 MS.gene31133:CDS 45.0%
AGAAGACGACGAAGAAGTGG+AGG + chr2.4:6362788-6362807 None:intergenic 50.0%
CCACCCAAATTGAGGATCAC+AGG - chr2.4:6363666-6363685 MS.gene31133:CDS 50.0%
CCTGTGATCCTCAATTTGGG+TGG + chr2.4:6363669-6363688 None:intergenic 50.0%
GAAGCACGTTTAGCAGGAAC+TGG - chr2.4:6363408-6363427 MS.gene31133:CDS 50.0%
GCACTTGAAGCACGTTTAGC+AGG - chr2.4:6363402-6363421 MS.gene31133:CDS 50.0%
GGCCAGAGACATATAGGCAA+TGG - chr2.4:6364168-6364187 MS.gene31133:CDS 50.0%
! GGTTATGGTTTCAAGTGGCC+TGG - chr2.4:6363609-6363628 MS.gene31133:CDS 50.0%
!!! AAATCCGAGGGGTGCTGTTT+TGG - chr2.4:6363905-6363924 MS.gene31133:CDS 50.0%
!!! TGAGGAAGAGTTTTGGGGGA+AGG - chr2.4:6364100-6364119 MS.gene31133:CDS 50.0%
GAAGAAGTGGAGGAAGGAGG+AGG + chr2.4:6362778-6362797 None:intergenic 55.0%
GACGAAGAAGTGGAGGAAGG+AGG + chr2.4:6362781-6362800 None:intergenic 55.0%
GACGACGAAGAAGTGGAGGA+AGG + chr2.4:6362784-6362803 None:intergenic 55.0%
GAAGTGGAGGAAGGAGGAGG+AGG + chr2.4:6362775-6362794 None:intergenic 60.0%
GCAGCAGCAGTAGCAGTAGG+AGG + chr2.4:6362751-6362770 None:intergenic 60.0%
GCAGCAGTAGCAGTAGGAGG+AGG + chr2.4:6362748-6362767 None:intergenic 60.0%
GCAGTAGCAGTAGGAGGAGG+AGG + chr2.4:6362745-6362764 None:intergenic 60.0%
GGAGCAGCAGCAGTAGCAGT+AGG + chr2.4:6362754-6362773 None:intergenic 60.0%
GTAGCAGTAGGAGGAGGAGG+AGG + chr2.4:6362742-6362761 None:intergenic 60.0%
GCAGTAGGAGGAGGAGGAGG+AGG + chr2.4:6362739-6362758 None:intergenic 65.0%
GTAGGAGGAGGAGGAGGAGG+AGG + chr2.4:6362736-6362755 None:intergenic 65.0%
! CTGGCGGGCCACCCAAATTG+AGG - chr2.4:6363658-6363677 MS.gene31133:CDS 65.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 6362667 6364379 6362667 ID=MS.gene31133
chr2.4 mRNA 6362667 6364379 6362667 ID=MS.gene31133.t1;Parent=MS.gene31133
chr2.4 exon 6362667 6364379 6362667 ID=MS.gene31133.t1.exon1;Parent=MS.gene31133.t1
chr2.4 CDS 6362667 6364379 6362667 ID=cds.MS.gene31133.t1;Parent=MS.gene31133.t1
Gene Sequence

>MS.gene31133

ATGTTCCAAGATTCCTTTGCTCTTCAACTTACTGAGAAATCATTTCATGATGTTATTTCTCACACTCCTCCTCCTCCTCCTCCTCCTACTGCTACTGCTGCTGCTCCTCCTCCTCCTTCCTCCACTTCTTCGTCGTCTTCTTCTTCTTCCATCCAGCATCAACAAAACTACAAATTTGTGCATAGCCCTGACATCAGTGATTATAGTTTCAGCAGTGTTAGCACTAGCTCCTTTGAACTTGATCCAATTATAGACTCATACAACTACAATTACAACCCTTTTCCACTCCCAAATACTCTTCCAATTCCTCCAAACTTTGTTTCTCAATTCGACTCAACTGTTTTCCCTTCTCATGAGGTAATTAAGACAGAAACCTTTGAAGAAGAGCATTTTCTCAATGTGTCACAAAATGAGTCAGAACAAGTATATGTAGATGATAACAGTGAGTTACCAGAATTATTTGATAAATTACTAGTCCTTGGTACTAAAGTAAAAAAAGGGCCACTTGAAAACACAAGCTTTCAGCAGAATGAAGAATTATCAAACAGATTCTATGGTTATAGGAGACAAAGAAGTTATGAGGAAGTTGTGGATTTGAGGACACTATTGATGCTATGTGCACAATCCATTTCTTGTAATGATATTTCAAATGCTAACCAGTTATTAAACCAGATTAAGACACATTCTTCTCCAACAGGTGATGGAACTCAAAGATTGGCACATTTCTTTGGAAATGCACTTGAAGCACGTTTAGCAGGAACTGGTTCACAAATCTATAGAGCTCTATCTTCCAAGAAGAAATCTGCTGCAGATATGGCAAGAGCTTACCAAGTTTATTCATCAGCATGCCCTTTTGAAAAGCTTGCAATTATGTTCTCAAACAATGCGATTTTTAATGTAGCAAAAGAAACAAAAAGTCTTCACATTATAGATTTTGGTGTTGGTTATGGTTTCAAGTGGCCTGGTTTAATACATCGTTTATCGAAAAGATCTGGCGGGCCACCCAAATTGAGGATCACAGGGATAGATCTTCCAAATTTGTTGGAGAGAGTGAATGGAACAGGTCTTAGGCTTGCAACTTATTGCGAACGTTTCGGTGTTCCTTTTGAGTTCAATGGTATTGCAAAAAATTGGGAAAGTATCAAAGTTGAGGATTTTAAGATAAGGAAAGATGAGTTTGTTGCTGTGAATTGTTATTTTAAACTTGAGAATCTACCTGATGAGACAGTTGTTTCGGAAAATCCGAGGGGTGCTGTTTTGGATTTGATTAAGAAGGCAAATCCAAATATATTTGTTCATAGCATTGTTAATGGTTGTTATGATGCACCTTTTTTTGTGACAAGGTTTAAGGAAGCTGTTTTTCATTACTCAGCATTGTTTGATATGCTTGATAACAACAATGTTGAACGTGAGGATCCTAATAGGTTGATGTTTGAGGAAGAGTTTTGGGGGAAGGATATAATGAATGTTATAGCTTGTGAAGGCTGTGATAGGATTGAGAGGCCAGAGACATATAGGCAATGGCATTTTAGGCACATGGGAAATGGGTTTAAGTCTTTGAAATTGGATAAGCATGTTATAGATAAATTGAAGGGTAAGTTGAGAGATGATGCATATAATAGTGATTTTCTGTTTGAGGTGAATAAAAATTGGATGCTACAAGGTTGGAAGGGTAGAGTTCTATTTGGTTCTTCTTGTTGGATTCCAGCATAG
Protein sequence

>MS.gene31133.t1

MFQDSFALQLTEKSFHDVISHTPPPPPPPTATAAAPPPPSSTSSSSSSSSIQHQQNYKFVHSPDISDYSFSSVSTSSFELDPIIDSYNYNYNPFPLPNTLPIPPNFVSQFDSTVFPSHEVIKTETFEEEHFLNVSQNESEQVYVDDNSELPELFDKLLVLGTKVKKGPLENTSFQQNEELSNRFYGYRRQRSYEEVVDLRTLLMLCAQSISCNDISNANQLLNQIKTHSSPTGDGTQRLAHFFGNALEARLAGTGSQIYRALSSKKKSAADMARAYQVYSSACPFEKLAIMFSNNAIFNVAKETKSLHIIDFGVGYGFKWPGLIHRLSKRSGGPPKLRITGIDLPNLLERVNGTGLRLATYCERFGVPFEFNGIAKNWESIKVEDFKIRKDEFVAVNCYFKLENLPDETVVSENPRGAVLDLIKKANPNIFVHSIVNGCYDAPFFVTRFKEAVFHYSALFDMLDNNNVEREDPNRLMFEEEFWGKDIMNVIACEGCDRIERPETYRQWHFRHMGNGFKSLKLDKHVIDKLKGKLRDDAYNSDFLFEVNKNWMLQGWKGRVLFGSSCWIPA