AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene31134

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31134.t1 MTR_2g097310 87.273 660 61 3 1 657 1 640 0.0 1143
MS.gene31134.t1 MTR_2g097390 80.572 664 101 4 1 657 1 643 0.0 1069
MS.gene31134.t1 MTR_2g097350 79.909 662 108 5 1 657 1 642 0.0 1046
MS.gene31134.t1 MTR_2g097380 78.788 495 56 5 168 657 113 563 0.0 788
MS.gene31134.t1 MTR_4g064160 61.239 436 157 5 232 657 253 686 0.0 560
MS.gene31134.t1 MTR_2g097410 40.316 697 317 18 46 657 61 743 3.40e-159 476
MS.gene31134.t1 MTR_4g064120 42.492 626 321 13 46 657 28 628 2.43e-157 467
MS.gene31134.t1 MTR_4g064150 41.416 664 322 15 46 657 87 735 5.93e-156 467
MS.gene31134.t1 MTR_4g064180 41.786 627 324 13 46 657 28 628 3.05e-153 457
MS.gene31134.t1 MTR_7g062120 50.508 394 189 3 268 657 351 742 1.33e-148 449
MS.gene31134.t1 MTR_4g064200 47.973 444 206 6 234 657 214 652 7.12e-144 433
MS.gene31134.t1 MTR_2g097467 38.365 636 334 15 50 657 51 656 2.38e-143 432
MS.gene31134.t1 MTR_2g097463 37.656 640 326 14 54 657 55 657 8.92e-142 428
MS.gene31134.t1 MTR_2g097473 38.295 645 322 15 50 657 51 656 2.51e-140 424
MS.gene31134.t1 MTR_5g097480 30.133 375 253 4 285 654 173 543 7.61e-60 210
MS.gene31134.t1 MTR_3g056110 28.810 538 347 15 136 654 21 541 1.21e-58 206
MS.gene31134.t1 MTR_2g026250 29.765 383 258 5 280 654 216 595 3.81e-57 204
MS.gene31134.t1 MTR_4g133660 30.423 378 253 6 284 655 170 543 9.16e-57 201
MS.gene31134.t1 MTR_7g027190 30.829 386 234 7 289 654 300 672 1.14e-55 201
MS.gene31134.t1 MTR_2g082090 30.214 374 254 3 285 654 208 578 1.31e-55 199
MS.gene31134.t1 MTR_3g089055 29.570 372 255 3 287 654 201 569 2.06e-53 193
MS.gene31134.t1 MTR_3g089055 29.570 372 255 3 287 654 196 564 2.21e-53 192
MS.gene31134.t1 MTR_5g094450 30.000 380 251 8 285 654 157 531 7.79e-53 190
MS.gene31134.t1 MTR_1g029420 29.301 372 256 3 287 654 223 591 2.43e-52 190
MS.gene31134.t1 MTR_8g442410 27.251 411 268 9 266 657 129 527 3.59e-47 175
MS.gene31134.t1 MTR_4g104020 27.177 379 255 8 289 654 149 519 6.87e-46 171
MS.gene31134.t1 MTR_7g074650 31.250 368 233 9 293 656 442 793 1.07e-45 174
MS.gene31134.t1 MTR_3g065980 29.659 381 238 8 290 654 173 539 2.71e-45 169
MS.gene31134.t1 MTR_4g102790 25.521 384 266 8 285 656 288 663 4.27e-43 165
MS.gene31134.t1 MTR_5g009080 25.850 441 261 9 275 654 42 477 1.86e-41 157
MS.gene31134.t1 MTR_3g022830 26.014 419 247 8 290 654 26 435 6.16e-39 149
MS.gene31134.t1 MTR_4g076140 24.648 426 256 7 289 654 48 468 6.34e-38 147
MS.gene31134.t1 MTR_4g097080 26.289 388 250 10 289 656 133 504 2.53e-37 146
MS.gene31134.t1 MTR_2g089100 27.603 413 236 11 289 657 52 445 4.09e-34 136
MS.gene31134.t1 MTR_1g069725 27.111 450 298 14 216 654 32 462 5.93e-34 135
MS.gene31134.t1 MTR_4g077760 26.882 372 246 9 294 654 198 554 9.57e-33 133
MS.gene31134.t1 MTR_2g034260 23.747 379 269 6 290 654 211 583 2.05e-32 132
MS.gene31134.t1 MTR_2g034250 25.066 379 265 7 290 654 211 584 5.09e-32 131
MS.gene31134.t1 MTR_5g015490 24.870 386 264 11 289 656 114 491 2.77e-30 125
MS.gene31134.t1 MTR_2g034280 23.873 377 269 7 290 654 204 574 3.12e-29 123
MS.gene31134.t1 MTR_1g096030 26.952 397 241 14 289 654 80 458 1.63e-28 119
MS.gene31134.t1 MTR_4g076020 27.005 374 234 12 293 657 88 431 2.68e-28 118
MS.gene31134.t1 MTR_7g057230 27.178 287 198 5 374 654 375 656 1.10e-27 119
MS.gene31134.t1 MTR_6g047750 25.729 377 257 8 290 654 296 661 1.44e-26 115
MS.gene31134.t1 MTR_7g109580 24.274 379 270 9 284 654 184 553 2.91e-26 114
MS.gene31134.t1 MTR_3g072710 24.311 399 252 14 289 655 120 500 9.87e-24 105
MS.gene31134.t1 MTR_2g099110 26.154 390 255 11 289 656 74 452 4.18e-23 103
MS.gene31134.t1 MTR_5g058860 25.065 387 262 12 289 655 119 497 9.66e-23 102
MS.gene31134.t1 MTR_1g086970 24.490 392 260 15 284 654 101 477 1.45e-22 102
MS.gene31134.t1 MTR_4g095500 22.967 418 267 11 289 657 59 470 4.94e-22 100
MS.gene31134.t1 MTR_5g015950 21.749 423 268 10 289 657 35 448 1.72e-21 98.2
MS.gene31134.t1 MTR_3g053270 21.749 423 268 10 289 657 35 448 1.72e-21 98.2
MS.gene31134.t1 MTR_8g093070 23.762 404 265 16 275 657 117 498 6.57e-18 87.8
MS.gene31134.t1 MTR_0092s0100 23.622 381 249 12 290 654 376 730 6.90e-16 82.0
MS.gene31134.t1 MTR_0092s0100 23.622 381 249 12 290 654 373 727 8.11e-16 81.6
MS.gene31134.t1 MTR_4g026485 23.450 371 253 12 294 654 274 623 1.91e-15 80.5
MS.gene31134.t1 MTR_2g097370 59.016 61 21 1 62 118 1 61 5.48e-13 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31134.t1 AT2G29060 42.188 640 334 10 50 656 57 693 3.73e-166 492
MS.gene31134.t1 AT2G29060 41.575 635 308 11 50 656 57 656 2.25e-165 489
MS.gene31134.t1 AT1G07530 51.392 467 205 9 209 657 305 767 3.15e-162 484
MS.gene31134.t1 AT2G29065 42.429 634 302 20 50 657 34 630 4.95e-145 436
MS.gene31134.t1 AT1G07520 49.213 445 205 10 227 657 94 531 4.69e-144 430
MS.gene31134.t1 AT1G07520 49.213 445 205 10 227 657 94 531 4.69e-144 430
MS.gene31134.t1 AT1G07520 49.213 445 205 10 227 657 258 695 1.18e-142 432
MS.gene31134.t1 AT1G07520 49.213 445 205 10 227 657 259 696 1.26e-142 432
MS.gene31134.t1 AT2G37650 47.368 380 192 4 281 656 327 702 2.27e-124 385
MS.gene31134.t1 AT2G37650 47.368 380 192 4 281 656 339 714 2.33e-124 385
MS.gene31134.t1 AT5G59450 40.315 444 253 5 225 657 158 600 4.08e-115 358
MS.gene31134.t1 AT3G46600 41.972 436 241 6 227 657 121 549 8.79e-115 355
MS.gene31134.t1 AT3G46600 41.972 436 241 6 227 657 153 581 1.67e-114 355
MS.gene31134.t1 AT3G46600 44.681 376 201 4 286 657 1 373 3.38e-112 342
MS.gene31134.t1 AT5G48150 31.888 392 259 5 268 654 101 489 3.86e-62 215
MS.gene31134.t1 AT5G48150 31.888 392 259 5 268 654 101 489 3.86e-62 215
MS.gene31134.t1 AT5G48150 31.888 392 259 5 268 654 101 489 3.86e-62 215
MS.gene31134.t1 AT5G48150 31.888 392 259 5 268 654 101 489 3.86e-62 215
MS.gene31134.t1 AT2G04890 30.811 370 250 3 285 654 49 412 6.92e-61 209
MS.gene31134.t1 AT1G50600 30.693 404 256 6 269 654 128 525 9.49e-59 206
MS.gene31134.t1 AT1G50600 30.693 404 256 6 269 654 199 596 2.03e-58 207
MS.gene31134.t1 AT1G50600 30.693 404 256 6 269 654 203 600 3.07e-58 207
MS.gene31134.t1 AT1G21450 28.989 376 253 7 287 654 223 592 3.42e-50 184
MS.gene31134.t1 AT3G54220 31.328 399 251 10 265 656 269 651 4.52e-48 179
MS.gene31134.t1 AT1G66350 30.570 386 233 11 279 654 145 505 4.75e-48 177
MS.gene31134.t1 AT2G01570 29.016 386 245 10 279 654 214 580 1.28e-46 174
MS.gene31134.t1 AT4G17230 28.117 377 264 3 284 656 153 526 2.93e-46 172
MS.gene31134.t1 AT4G17230 28.117 377 264 3 284 656 153 526 2.93e-46 172
MS.gene31134.t1 AT1G14920 27.749 382 255 7 279 654 162 528 1.76e-45 170
MS.gene31134.t1 AT3G03450 27.532 385 257 8 279 654 173 544 7.12e-45 168
MS.gene31134.t1 AT5G17490 25.815 368 257 7 290 654 161 515 6.75e-42 159
MS.gene31134.t1 AT1G50420 22.664 428 263 9 289 654 57 478 3.28e-36 142
MS.gene31134.t1 AT5G66770 29.679 374 237 11 293 654 224 583 5.28e-36 143
MS.gene31134.t1 AT1G63100 28.857 350 228 9 289 629 277 614 1.38e-35 143
MS.gene31134.t1 AT1G63100 28.857 350 228 9 289 629 252 589 1.46e-35 142
MS.gene31134.t1 AT4G37650 25.833 360 233 9 288 622 145 495 5.62e-34 137
MS.gene31134.t1 AT5G41920 29.091 385 236 14 284 654 38 399 6.84e-33 131
MS.gene31134.t1 AT3G50650 26.870 361 235 9 309 654 195 541 9.02e-31 127
MS.gene31134.t1 AT3G49950 26.221 389 257 9 289 656 30 409 2.43e-28 118
MS.gene31134.t1 AT5G52510 24.422 389 258 9 287 654 266 639 2.34e-26 114
MS.gene31134.t1 AT1G55580 24.760 416 254 12 286 654 41 444 1.14e-25 110
MS.gene31134.t1 AT4G08250 25.208 361 244 12 308 654 128 476 1.58e-24 108
MS.gene31134.t1 AT3G13840 24.219 384 251 11 289 654 148 509 1.86e-12 70.5

Find 109 sgRNAs with CRISPR-Local

Find 133 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTAATCTGATTTAATATT+TGG 0.081879 2.4:+6385303 None:intergenic
TTCTATTTGGTTCTTCTTGC+TGG 0.149493 2.4:-6384281 MS.gene31134:CDS
TTCACTCTCTCCATTGAATT+TGG 0.151096 2.4:+6384927 None:intergenic
AGTCTTCACATTATAGATTT+TGG 0.160984 2.4:-6385044 MS.gene31134:CDS
AATCTTCTTCCATGAGAAAT+TGG 0.197500 2.4:+6386047 None:intergenic
ATGAGGAAGTTGTGGATTTC+AGG 0.206069 2.4:-6385382 MS.gene31134:CDS
TATCCCTGTGATCTTCAATT+TGG 0.207790 2.4:+6384956 None:intergenic
GAATTCACTTCAACTGTTCT+TGG 0.234393 2.4:-6386175 MS.gene31134:CDS
TGGGTTTAAGTCTTTGAAAT+TGG 0.243482 2.4:-6384415 MS.gene31134:CDS
TTTATTCATAGCATTGTTAA+TGG 0.246687 2.4:-6384669 MS.gene31134:CDS
CAAAGATTGGCACATTTCTA+TGG 0.249724 2.4:-6385251 MS.gene31134:CDS
GAATCCGAAGGGTGCTGTTT+TGG 0.259645 2.4:-6384721 MS.gene31134:CDS
TGAAATATCATTACAAGAAA+TGG 0.276552 2.4:+6385334 None:intergenic
TTATTTGATAAATTACTTCT+TGG 0.283709 2.4:-6385500 MS.gene31134:CDS
CATATAGGCATTGGCATTCT+AGG 0.286084 2.4:-6384449 MS.gene31134:CDS
CCAAATATTAAATCAGATTA+AGG 0.286177 2.4:-6385303 MS.gene31134:CDS
TAGTTGTATGAGTCTATAAT+TGG 0.294656 2.4:+6385722 None:intergenic
ATTCACTCTTACTTTGTTCA+TGG 0.296917 2.4:+6386194 None:intergenic
TGATGTTTGAGGAAGTGTTT+TGG 0.302555 2.4:-6384533 MS.gene31134:CDS
TGTTTGAGGTGAAGGAAAAT+TGG 0.305166 2.4:-6384329 MS.gene31134:CDS
TGGAAGGGTAGAATTCTATT+TGG 0.313548 2.4:-6384294 MS.gene31134:CDS
GGAGGGAATGGAAGAGTAGT+TGG 0.316336 2.4:+6385677 None:intergenic
CATTCTAGGCACGTGGGAAA+TGG 0.320991 2.4:-6384435 MS.gene31134:CDS
ATCCCTGTGATCTTCAATTT+GGG 0.345121 2.4:+6384957 None:intergenic
TCCTCAAACATCAACCTATT+AGG 0.361937 2.4:+6384543 None:intergenic
TAGCTTGTGAAGGTTGTGAT+AGG 0.367130 2.4:-6384488 MS.gene31134:CDS
GAGTGAATGAAGCAGGTCTT+AGG 0.373688 2.4:-6384911 MS.gene31134:CDS
TCTATTTGGTTCTTCTTGCT+GGG 0.374895 2.4:-6384280 MS.gene31134:CDS
CATTATTGAAAGAAGGGAAA+AGG 0.380669 2.4:+6385624 None:intergenic
ATGCTATGAATAAATATATT+CGG 0.399119 2.4:+6384678 None:intergenic
GCAATACAATTGAACTCAAA+AGG 0.401581 2.4:+6384858 None:intergenic
GTTGAAGAGCATAGGAATCT+TGG 0.409119 2.4:+6386002 None:intergenic
TTGAGAGGCCAGAGACATAT+AGG 0.413255 2.4:-6384464 MS.gene31134:CDS
AGAAGGGAAAAGGGTTGGAT+TGG 0.418599 2.4:+6385634 None:intergenic
TATAATTGGATCAAGTTCAT+AGG 0.422425 2.4:+6385736 None:intergenic
GAAAGAGCATTATTGAAAGA+AGG 0.427798 2.4:+6385617 None:intergenic
GTGAACGTGAGGATCCTAAT+AGG 0.434511 2.4:-6384557 MS.gene31134:CDS
AAAGAGCATTATTGAAAGAA+GGG 0.443472 2.4:+6385618 None:intergenic
AAGGAACATTCTTATCCAAC+AGG 0.444390 2.4:-6385284 MS.gene31134:CDS
ACTCGATGAGACAGTAGTTT+CGG 0.444774 2.4:-6384745 MS.gene31134:CDS
AGACGAAGAAGAGGAAGAAG+AGG 0.446816 2.4:+6385898 None:intergenic
TTGAAAGAAGGGAAAAGGGT+TGG 0.454471 2.4:+6385629 None:intergenic
CTTTGAGTTCCATCACCTGT+TGG 0.458988 2.4:+6385269 None:intergenic
CTCTTACTTTGTTCATGGTG+TGG 0.459748 2.4:+6386199 None:intergenic
GATAGATCTTCCAAATTCAA+TGG 0.460382 2.4:-6384937 MS.gene31134:CDS
GCAGCAGCAGTAGCAGCAGT+AGG 0.461250 2.4:+6385938 None:intergenic
TAGGAGAAAGAGAAGTTATG+AGG 0.463600 2.4:-6385399 MS.gene31134:CDS
ATTCTAGGCACGTGGGAAAT+GGG 0.464304 2.4:-6384434 MS.gene31134:CDS
AGAATAAGAAGACGAAGAAG+AGG 0.467187 2.4:+6385889 None:intergenic
AAACTATAATCACTGAAGCT+AGG 0.467624 2.4:+6385779 None:intergenic
GAAGCACGTTTAGCAGGAAC+TGG 0.473702 2.4:-6385218 MS.gene31134:CDS
TCCTAATAGGTTGATGTTTG+AGG 0.474185 2.4:-6384544 MS.gene31134:CDS
GAGGGAATGGAAGAGTAGTT+GGG 0.474502 2.4:+6385678 None:intergenic
ATTATTGAAAGAAGGGAAAA+GGG 0.478667 2.4:+6385625 None:intergenic
GAGAAGTTATGAGGAAGTTG+TGG 0.483254 2.4:-6385390 MS.gene31134:CDS
TGCCAATGCCTATATGTCTC+TGG 0.483967 2.4:+6384456 None:intergenic
ACTCTTCATGCTCATTGTGT+TGG 0.485254 2.4:+6386095 None:intergenic
GCAGTAGCAGCAGTAGGAGG+AGG 0.489711 2.4:+6385944 None:intergenic
GCAGATTATAGATAAATTGA+AGG 0.495433 2.4:-6384388 MS.gene31134:CDS
GACGAAGAAGAGGAAGAAGA+GGG 0.495930 2.4:+6385899 None:intergenic
GAAGAATTATCAAACAGATT+CGG 0.502364 2.4:-6385428 MS.gene31134:CDS
CCACCCAAATTGAAGATCAC+AGG 0.507118 2.4:-6384960 MS.gene31134:CDS
AAAATTGGATGCTACAAGGT+TGG 0.509330 2.4:-6384314 MS.gene31134:CDS
AAAATGTGATTAGGAGGGAA+TGG 0.510292 2.4:+6385665 None:intergenic
CTTCAATTTGGGTGGCCCAC+CGG 0.513811 2.4:+6384968 None:intergenic
CATATTGTGAAGGTCTATGC+TGG 0.514011 2.4:+6384255 None:intergenic
GTTGATATAACCAAGAGCAT+TGG 0.514520 2.4:+6386069 None:intergenic
AACTATAATCACTGAAGCTA+GGG 0.515859 2.4:+6385780 None:intergenic
TCGATGGACAAAAGAGTTGT+TGG 0.516333 2.4:-6386223 MS.gene31134:CDS
GCACTCGAAGCACGTTTAGC+AGG 0.522176 2.4:-6385224 MS.gene31134:CDS
GAAGAGTCATCCAATGCTCT+TGG 0.522768 2.4:-6386079 MS.gene31134:CDS
CATTCTTATCCAACAGGTGA+TGG 0.523595 2.4:-6385278 MS.gene31134:CDS
GGCCAGAGACATATAGGCAT+TGG 0.526736 2.4:-6384458 MS.gene31134:CDS
GGAAGAAGAGGGAAGAGTAG+TGG 0.529525 2.4:+6385910 None:intergenic
TTATATCAACCAATTTCTCA+TGG 0.530130 2.4:-6386056 MS.gene31134:CDS
AAATCCAAAACAGCACCCTT+CGG 0.534191 2.4:+6384717 None:intergenic
CACCGTCTATCGAAAAGATC+CGG 0.539937 2.4:-6384987 MS.gene31134:CDS
CTTGGTACTAAAGTCGCAAA+AGG 0.541250 2.4:-6385482 MS.gene31134:CDS
CAATAATGCTCTTTCTCACG+AGG 0.541791 2.4:-6385609 MS.gene31134:CDS
GGCATGCTGATGAATAAACT+TGG 0.547453 2.4:+6385132 None:intergenic
TTGGATGCTACAAGGTTGGA+AGG 0.548278 2.4:-6384310 MS.gene31134:CDS
TTGGGAAAGTATCAAAGTTG+AGG 0.550259 2.4:-6384829 MS.gene31134:CDS
CAGATTATAGATAAATTGAA+GGG 0.550818 2.4:-6384387 MS.gene31134:CDS
AGCTTGTGAAGGTTGTGATA+GGG 0.552272 2.4:-6384487 MS.gene31134:CDS
ATGGAGAGAGTGAATGAAGC+AGG 0.553640 2.4:-6384918 MS.gene31134:CDS
TTATAGATAAATTGAAGGGT+AGG 0.554979 2.4:-6384383 MS.gene31134:CDS
AGGTGATGGAACTCAAAGAT+TGG 0.559955 2.4:-6385264 MS.gene31134:CDS
CCTGTGATCTTCAATTTGGG+TGG 0.565831 2.4:+6384960 None:intergenic
GATAGACGGTGTATAAAAGC+AGG 0.571505 2.4:+6384999 None:intergenic
GCAGCAGTAGCAGCAGTAGG+AGG 0.573244 2.4:+6385941 None:intergenic
GAATTATCAAACAGATTCGG+TGG 0.598197 2.4:-6385425 MS.gene31134:CDS
CACCCAAATTGAAGATCACA+GGG 0.598988 2.4:-6384959 MS.gene31134:CDS
CTCAGTAAGTTGAAGAGCAT+AGG 0.600415 2.4:+6385994 None:intergenic
CAGTAGCAGCAGTAGGAGGA+GGG 0.605945 2.4:+6385945 None:intergenic
AAGGAAAATTGGATGCTACA+AGG 0.606455 2.4:-6384318 MS.gene31134:CDS
GTGTTGGTTATGGTTTCAAG+TGG 0.612035 2.4:-6385022 MS.gene31134:CDS
AGTAGTTTCGGAGAATCCGA+AGG 0.616125 2.4:-6384733 MS.gene31134:CDS
ATTCATAGCATTGTTAATGG+CGG 0.619293 2.4:-6384666 MS.gene31134:CDS
CGTCTATCGAAAAGATCCGG+TGG 0.631429 2.4:-6384984 MS.gene31134:CDS
GTCTATCGAAAAGATCCGGT+GGG 0.632717 2.4:-6384983 MS.gene31134:CDS
ATGAATGTTATAGCTTGTGA+AGG 0.633082 2.4:-6384498 MS.gene31134:CDS
AGTGATTATAGTTTCAGCAG+TGG 0.635543 2.4:-6385770 MS.gene31134:CDS
AGAAGAGGGAAGAGTAGTGG+CGG 0.638209 2.4:+6385913 None:intergenic
TGGATGCTACAAGGTTGGAA+GGG 0.641743 2.4:-6384309 MS.gene31134:CDS
GCATTGGCATTCTAGGCACG+TGG 0.643333 2.4:-6384442 MS.gene31134:CDS
TAACAACAATAGTGAACGTG+AGG 0.667397 2.4:-6384568 MS.gene31134:CDS
CATTGGCATTCTAGGCACGT+GGG 0.682240 2.4:-6384441 MS.gene31134:CDS
AAGGTTGTGATAGGGTTGAG+AGG 0.698263 2.4:-6384479 MS.gene31134:CDS
GTAGTTTCGGAGAATCCGAA+GGG 0.706195 2.4:-6384732 MS.gene31134:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGCTATGAATAAATATATT+CGG + chr2.4:6385814-6385833 None:intergenic 15.0%
!! TTATTTGATAAATTACTTCT+TGG - chr2.4:6384989-6385008 MS.gene31134:CDS 15.0%
!!! TTTTATAAACAAATTTCTCA+TGG - chr2.4:6384337-6384356 MS.gene31134:CDS 15.0%
!! CAATTGTATTGCAAAAAATT+GGG - chr2.4:6385642-6385661 MS.gene31134:CDS 20.0%
!! CAGATTATAGATAAATTGAA+GGG - chr2.4:6386102-6386121 MS.gene31134:CDS 20.0%
!! CCAAATATTAAATCAGATTA+AGG - chr2.4:6385186-6385205 MS.gene31134:CDS 20.0%
!! CCTTAATCTGATTTAATATT+TGG + chr2.4:6385189-6385208 None:intergenic 20.0%
!! TCAATTGTATTGCAAAAAAT+TGG - chr2.4:6385641-6385660 MS.gene31134:CDS 20.0%
!! TGAAATATCATTACAAGAAA+TGG + chr2.4:6385158-6385177 None:intergenic 20.0%
!! TTTATTCATAGCATTGTTAA+TGG - chr2.4:6385820-6385839 MS.gene31134:CDS 20.0%
! ATTATTGAAAGAAGGGAAAA+GGG + chr2.4:6384867-6384886 None:intergenic 25.0%
! GAAGAATTATCAAACAGATT+CGG - chr2.4:6385061-6385080 MS.gene31134:CDS 25.0%
! GATTGAGAAAAAATGTGATT+AGG + chr2.4:6384836-6384855 None:intergenic 25.0%
! GCAGATTATAGATAAATTGA+AGG - chr2.4:6386101-6386120 MS.gene31134:CDS 25.0%
! TAGTTGTATGAGTCTATAAT+TGG + chr2.4:6384770-6384789 None:intergenic 25.0%
! TATAATTGGATCAAGTTCAT+AGG + chr2.4:6384756-6384775 None:intergenic 25.0%
! TTATAGATAAATTGAAGGGT+AGG - chr2.4:6386106-6386125 MS.gene31134:CDS 25.0%
! TTATATCAACCAATTTCTCA+TGG - chr2.4:6384433-6384452 MS.gene31134:CDS 25.0%
!! AGTCTTCACATTATAGATTT+TGG - chr2.4:6385445-6385464 MS.gene31134:CDS 25.0%
!! ATAATTGCAAGCTTTTCAAA+AGG + chr2.4:6385382-6385401 None:intergenic 25.0%
!! TAATTGCAAGCTTTTCAAAA+GGG + chr2.4:6385381-6385400 None:intergenic 25.0%
!!! AAAGAGCATTATTGAAAGAA+GGG + chr2.4:6384874-6384893 None:intergenic 25.0%
!!! AAGTTGAGGATTTTAAGATA+AGG - chr2.4:6385674-6385693 MS.gene31134:CDS 25.0%
AAACTATAATCACTGAAGCT+AGG + chr2.4:6384713-6384732 None:intergenic 30.0%
AACTATAATCACTGAAGCTA+GGG + chr2.4:6384712-6384731 None:intergenic 30.0%
AATCTTCTTCCATGAGAAAT+TGG + chr2.4:6384445-6384464 None:intergenic 30.0%
ATTCACTCTTACTTTGTTCA+TGG + chr2.4:6384298-6384317 None:intergenic 30.0%
ATTCATAGCATTGTTAATGG+CGG - chr2.4:6385823-6385842 MS.gene31134:CDS 30.0%
CATTATTGAAAGAAGGGAAA+AGG + chr2.4:6384868-6384887 None:intergenic 30.0%
GAGAAAAAATGTGATTAGGA+GGG + chr2.4:6384832-6384851 None:intergenic 30.0%
GATAGATCTTCCAAATTCAA+TGG - chr2.4:6385552-6385571 MS.gene31134:CDS 30.0%
GCAATACAATTGAACTCAAA+AGG + chr2.4:6385634-6385653 None:intergenic 30.0%
TGAGAAAAAATGTGATTAGG+AGG + chr2.4:6384833-6384852 None:intergenic 30.0%
TTAAACCTCGTCACAAAAAA+AGG + chr2.4:6385859-6385878 None:intergenic 30.0%
! ATGAATGTTATAGCTTGTGA+AGG - chr2.4:6385991-6386010 MS.gene31134:CDS 30.0%
! TAACAGTGATTTTCTGTTTG+AGG - chr2.4:6386146-6386165 MS.gene31134:CDS 30.0%
!! CACATTATAGATTTTGGTGT+TGG - chr2.4:6385451-6385470 MS.gene31134:CDS 30.0%
!! GAAAGAGCATTATTGAAAGA+AGG + chr2.4:6384875-6384894 None:intergenic 30.0%
!! TGGGTTTAAGTCTTTGAAAT+TGG - chr2.4:6386074-6386093 MS.gene31134:CDS 30.0%
!!! ATAGATTTTGGTGTTGGTTA+TGG - chr2.4:6385457-6385476 MS.gene31134:CDS 30.0%
!!! TTTTTTTGTGACGAGGTTTA+AGG - chr2.4:6385858-6385877 MS.gene31134:CDS 30.0%
AAAATGTGATTAGGAGGGAA+TGG + chr2.4:6384827-6384846 None:intergenic 35.0%
AAAATTGGATGCTACAAGGT+TGG - chr2.4:6386175-6386194 MS.gene31134:CDS 35.0%
AAGGAAAATTGGATGCTACA+AGG - chr2.4:6386171-6386190 MS.gene31134:CDS 35.0%
AAGGAACATTCTTATCCAAC+AGG - chr2.4:6385205-6385224 MS.gene31134:CDS 35.0%
AGAATAAGAAGACGAAGAAG+AGG + chr2.4:6384603-6384622 None:intergenic 35.0%
AGTGATTATAGTTTCAGCAG+TGG - chr2.4:6384719-6384738 MS.gene31134:CDS 35.0%
ATCCCTGTGATCTTCAATTT+GGG + chr2.4:6385535-6385554 None:intergenic 35.0%
CAAAGATTGGCACATTTCTA+TGG - chr2.4:6385238-6385257 MS.gene31134:CDS 35.0%
GAAAAAATCTGCTGCAGATA+TGG - chr2.4:6385324-6385343 MS.gene31134:CDS 35.0%
GAATTATCAAACAGATTCGG+TGG - chr2.4:6385064-6385083 MS.gene31134:CDS 35.0%
GAATTCACTTCAACTGTTCT+TGG - chr2.4:6384314-6384333 MS.gene31134:CDS 35.0%
TAACAACAATAGTGAACGTG+AGG - chr2.4:6385921-6385940 MS.gene31134:CDS 35.0%
TAGGAGAAAGAGAAGTTATG+AGG - chr2.4:6385090-6385109 MS.gene31134:CDS 35.0%
TATCCCTGTGATCTTCAATT+TGG + chr2.4:6385536-6385555 None:intergenic 35.0%
TCCTCAAACATCAACCTATT+AGG + chr2.4:6385949-6385968 None:intergenic 35.0%
TGGAAGGGTAGAATTCTATT+TGG - chr2.4:6386195-6386214 MS.gene31134:CDS 35.0%
TTCACTCTCTCCATTGAATT+TGG + chr2.4:6385565-6385584 None:intergenic 35.0%
! GTTGATATAACCAAGAGCAT+TGG + chr2.4:6384423-6384442 None:intergenic 35.0%
! TGTTTGAGGTGAAGGAAAAT+TGG - chr2.4:6386160-6386179 MS.gene31134:CDS 35.0%
!! TCCTAATAGGTTGATGTTTG+AGG - chr2.4:6385945-6385964 MS.gene31134:CDS 35.0%
!! TCTATTTGGTTCTTCTTGCT+GGG - chr2.4:6386209-6386228 MS.gene31134:CDS 35.0%
!! TGATTTTCTGTTTGAGGTGA+AGG - chr2.4:6386152-6386171 MS.gene31134:CDS 35.0%
!! TTCTATTTGGTTCTTCTTGC+TGG - chr2.4:6386208-6386227 MS.gene31134:CDS 35.0%
!! TTGGGAAAGTATCAAAGTTG+AGG - chr2.4:6385660-6385679 MS.gene31134:CDS 35.0%
!!! ATCTGCAGCAGATTTTTTCT+TGG + chr2.4:6385323-6385342 None:intergenic 35.0%
!!! ATGTTTGAGGAAGTGTTTTG+GGG - chr2.4:6385958-6385977 MS.gene31134:CDS 35.0%
!!! GATGTTTGAGGAAGTGTTTT+GGG - chr2.4:6385957-6385976 MS.gene31134:CDS 35.0%
!!! TGAAAGCTTGTGTTTTGAAG+TGG + chr2.4:6385034-6385053 None:intergenic 35.0%
!!! TGATGTTTGAGGAAGTGTTT+TGG - chr2.4:6385956-6385975 MS.gene31134:CDS 35.0%
AAATCCAAAACAGCACCCTT+CGG + chr2.4:6385775-6385794 None:intergenic 40.0%
ACTCGATGAGACAGTAGTTT+CGG - chr2.4:6385744-6385763 MS.gene31134:CDS 40.0%
ACTCTTCATGCTCATTGTGT+TGG + chr2.4:6384397-6384416 None:intergenic 40.0%
AGCTTGTGAAGGTTGTGATA+GGG - chr2.4:6386002-6386021 MS.gene31134:CDS 40.0%
ATGAGGAAGTTGTGGATTTC+AGG - chr2.4:6385107-6385126 MS.gene31134:CDS 40.0%
CAATAATGCTCTTTCTCACG+AGG - chr2.4:6384880-6384899 MS.gene31134:CDS 40.0%
CACCCAAATTGAAGATCACA+GGG - chr2.4:6385530-6385549 MS.gene31134:CDS 40.0%
CATTCTTATCCAACAGGTGA+TGG - chr2.4:6385211-6385230 MS.gene31134:CDS 40.0%
CTCTTACTTTGTTCATGGTG+TGG + chr2.4:6384293-6384312 None:intergenic 40.0%
CTTGGTACTAAAGTCGCAAA+AGG - chr2.4:6385007-6385026 MS.gene31134:CDS 40.0%
GAGAAGTTATGAGGAAGTTG+TGG - chr2.4:6385099-6385118 MS.gene31134:CDS 40.0%
GATAGACGGTGTATAAAAGC+AGG + chr2.4:6385493-6385512 None:intergenic 40.0%
GGCATGCTGATGAATAAACT+TGG + chr2.4:6385360-6385379 None:intergenic 40.0%
TTGAAAGAAGGGAAAAGGGT+TGG + chr2.4:6384863-6384882 None:intergenic 40.0%
! CTCAGTAAGTTGAAGAGCAT+AGG + chr2.4:6384498-6384517 None:intergenic 40.0%
! GTTGAAGAGCATAGGAATCT+TGG + chr2.4:6384490-6384509 None:intergenic 40.0%
! TAGCTTGTGAAGGTTGTGAT+AGG - chr2.4:6386001-6386020 MS.gene31134:CDS 40.0%
!! AGGTGATGGAACTCAAAGAT+TGG - chr2.4:6385225-6385244 MS.gene31134:CDS 40.0%
!! AGTTGTAGTTTTGCTGATGC+TGG + chr2.4:6384679-6384698 None:intergenic 40.0%
!! CATATAGGCATTGGCATTCT+AGG - chr2.4:6386040-6386059 MS.gene31134:CDS 40.0%
!! GTGTTGGTTATGGTTTCAAG+TGG - chr2.4:6385467-6385486 MS.gene31134:CDS 40.0%
!!! ATGCACCTTTTTTTGTGACG+AGG - chr2.4:6385851-6385870 MS.gene31134:CDS 40.0%
!!! CAAACAGATTCGGTGGTTTT+AGG - chr2.4:6385071-6385090 MS.gene31134:CDS 40.0%
!!! TGTTTGAGGAAGTGTTTTGG+GGG - chr2.4:6385959-6385978 MS.gene31134:CDS 40.0%
AAGGTTGTGATAGGGTTGAG+AGG - chr2.4:6386010-6386029 MS.gene31134:CDS 45.0%
AGAAGGGAAAAGGGTTGGAT+TGG + chr2.4:6384858-6384877 None:intergenic 45.0%
AGACGAAGAAGAGGAAGAAG+AGG + chr2.4:6384594-6384613 None:intergenic 45.0%
AGTAGTTTCGGAGAATCCGA+AGG - chr2.4:6385756-6385775 MS.gene31134:CDS 45.0%
ATGGAGAGAGTGAATGAAGC+AGG - chr2.4:6385571-6385590 MS.gene31134:CDS 45.0%
ATTCTAGGCACGTGGGAAAT+GGG - chr2.4:6386055-6386074 MS.gene31134:CDS 45.0%
CACCGTCTATCGAAAAGATC+CGG - chr2.4:6385502-6385521 MS.gene31134:CDS 45.0%
CCACCCAAATTGAAGATCAC+AGG - chr2.4:6385529-6385548 MS.gene31134:CDS 45.0%
CCTGTGATCTTCAATTTGGG+TGG + chr2.4:6385532-6385551 None:intergenic 45.0%
GAAGAGTCATCCAATGCTCT+TGG - chr2.4:6384410-6384429 MS.gene31134:CDS 45.0%
GACGAAGAAGAGGAAGAAGA+GGG + chr2.4:6384593-6384612 None:intergenic 45.0%
GAGGGAATGGAAGAGTAGTT+GGG + chr2.4:6384814-6384833 None:intergenic 45.0%
GAGTGAATGAAGCAGGTCTT+AGG - chr2.4:6385578-6385597 MS.gene31134:CDS 45.0%
GTAGTTTCGGAGAATCCGAA+GGG - chr2.4:6385757-6385776 MS.gene31134:CDS 45.0%
GTCTATCGAAAAGATCCGGT+GGG - chr2.4:6385506-6385525 MS.gene31134:CDS 45.0%
GTGAACGTGAGGATCCTAAT+AGG - chr2.4:6385932-6385951 MS.gene31134:CDS 45.0%
TGCCAATGCCTATATGTCTC+TGG + chr2.4:6386036-6386055 None:intergenic 45.0%
TGGATGCTACAAGGTTGGAA+GGG - chr2.4:6386180-6386199 MS.gene31134:CDS 45.0%
TTGAGAGGCCAGAGACATAT+AGG - chr2.4:6386025-6386044 MS.gene31134:CDS 45.0%
TTGGATGCTACAAGGTTGGA+AGG - chr2.4:6386179-6386198 MS.gene31134:CDS 45.0%
! CACCGGATCTTTTCGATAGA+CGG + chr2.4:6385507-6385526 None:intergenic 45.0%
! CTTTGAGTTCCATCACCTGT+TGG + chr2.4:6385223-6385242 None:intergenic 45.0%
!! GTAGTTTTGCTGATGCTGGA+TGG + chr2.4:6384675-6384694 None:intergenic 45.0%
AGAAGAGGGAAGAGTAGTGG+CGG + chr2.4:6384579-6384598 None:intergenic 50.0%
CATTCTAGGCACGTGGGAAA+TGG - chr2.4:6386054-6386073 MS.gene31134:CDS 50.0%
CATTGGCATTCTAGGCACGT+GGG - chr2.4:6386048-6386067 MS.gene31134:CDS 50.0%
CGTCTATCGAAAAGATCCGG+TGG - chr2.4:6385505-6385524 MS.gene31134:CDS 50.0%
GAAGCACGTTTAGCAGGAAC+TGG - chr2.4:6385271-6385290 MS.gene31134:CDS 50.0%
GGAAGAAGAGGGAAGAGTAG+TGG + chr2.4:6384582-6384601 None:intergenic 50.0%
GGAGGGAATGGAAGAGTAGT+TGG + chr2.4:6384815-6384834 None:intergenic 50.0%
GGCCAGAGACATATAGGCAT+TGG - chr2.4:6386031-6386050 MS.gene31134:CDS 50.0%
!!! GAATCCGAAGGGTGCTGTTT+TGG - chr2.4:6385768-6385787 MS.gene31134:CDS 50.0%
CAGTAGCAGCAGTAGGAGGA+GGG + chr2.4:6384547-6384566 None:intergenic 55.0%
CTTCAATTTGGGTGGCCCAC+CGG + chr2.4:6385524-6385543 None:intergenic 55.0%
GCACTCGAAGCACGTTTAGC+AGG - chr2.4:6385265-6385284 MS.gene31134:CDS 55.0%
! GCATTGGCATTCTAGGCACG+TGG - chr2.4:6386047-6386066 MS.gene31134:CDS 55.0%
GCAGCAGCAGTAGCAGCAGT+AGG + chr2.4:6384554-6384573 None:intergenic 60.0%
GCAGCAGTAGCAGCAGTAGG+AGG + chr2.4:6384551-6384570 None:intergenic 60.0%
GCAGTAGCAGCAGTAGGAGG+AGG + chr2.4:6384548-6384567 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 6384269 6386242 6384269 ID=MS.gene31134
chr2.4 mRNA 6384269 6386242 6384269 ID=MS.gene31134.t1;Parent=MS.gene31134
chr2.4 exon 6384269 6386242 6384269 ID=MS.gene31134.t1.exon1;Parent=MS.gene31134.t1
chr2.4 CDS 6384269 6386242 6384269 ID=cds.MS.gene31134.t1;Parent=MS.gene31134.t1
Gene Sequence

>MS.gene31134

ATGGACAAAAGAGTTGTTGGTCCACACCATGAACAAAGTAAGAGTGAATTCACTTCAACTGTTCTTGGTTTTATAAACAAATTTCTCATGGAAGATGATGACATAGAAGAAGACTACAATATGTTCCAACACAATGAGCATGAAGAGTCATCCAATGCTCTTGGTTATATCAACCAATTTCTCATGGAAGAAGATTTTGAAAAAGAATACACTATGTTCCAAGATTCCTATGCTCTTCAACTTACTGAGAAATCATTTCATGATGTTATTTCTCACCCTCCTCCTACTGCTGCTACTGCTGCTGCTGCCGCCACTACTCTTCCCTCTTCTTCCTCTTCTTCGTCTTCTTATTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCCATCCAGCATCAGCAAAACTACAACTATGTGCATAGCCCTAGCTTCAGTGATTATAGTTTCAGCAGTGGTAGCACTAGCTCCTATGAACTTGATCCAATTATAGACTCATACAACTATAATAACAACGCTTTTCTACTCCCAACTACTCTTCCATTCCCTCCTAATCACATTTTTTCTCAATCCAATCCAACCCTTTTCCCTTCTTTCAATAATGCTCTTTCTCACGAGGTAATTAAGATAGAAAACTTTGAAGAAGAGCATTTTCTTAATGTGTCAAAACAAGTGAATGTAGATGATAACAGTGAGTTATCAGAATTATTTGATAAATTACTTCTTGGTACTAAAGTCGCAAAAGGTCCACTTCAAAACACAAGCTTTCAGCAGAATGAAGAATTATCAAACAGATTCGGTGGTTTTAGGAGAAAGAGAAGTTATGAGGAAGTTGTGGATTTCAGGACACTATTGATGCTATGTGCACAATCCATTTCTTGTAATGATATTTCAAATGCTAACCAAATATTAAATCAGATTAAGGAACATTCTTATCCAACAGGTGATGGAACTCAAAGATTGGCACATTTCTATGGAAATGCACTCGAAGCACGTTTAGCAGGAACTGGTTCACTTATTTATAGAGCTCTATCTTCCAAGAAAAAATCTGCTGCAGATATGGCAAGAGCTTACCAAGTTTATTCATCAGCATGCCCTTTTGAAAAGCTTGCAATTATGTTCTCAAACAATGCAATTTTCAATGTAGCAAAAGAAACAGAAAGTCTTCACATTATAGATTTTGGTGTTGGTTATGGTTTCAAGTGGCCTGCTTTTATACACCGTCTATCGAAAAGATCCGGTGGGCCACCCAAATTGAAGATCACAGGGATAGATCTTCCAAATTCAATGGAGAGAGTGAATGAAGCAGGTCTTAGGCTTGCAAGTTATTGCAAACGTTTCAATGTTCCTTTTGAGTTCAATTGTATTGCAAAAAATTGGGAAAGTATCAAAGTTGAGGATTTTAAGATAAGGAAAAATGAGTTTGTTGCTGTGAATTGTTTGTTTAAGTTTGAGAATCTACTCGATGAGACAGTAGTTTCGGAGAATCCGAAGGGTGCTGTTTTGGATTTGATTAGAAAGACAAATCCGAATATATTTATTCATAGCATTGTTAATGGCGGTTATGATGCACCTTTTTTTGTGACGAGGTTTAAGGAAGCTGTTTTTCATTATTCAGCATTGTTTGATACGCTTGATAACAACAATAGTGAACGTGAGGATCCTAATAGGTTGATGTTTGAGGAAGTGTTTTGGGGGAAAGATATAATGAATGTTATAGCTTGTGAAGGTTGTGATAGGGTTGAGAGGCCAGAGACATATAGGCATTGGCATTCTAGGCACGTGGGAAATGGGTTTAAGTCTTTGAAATTGGATAAGCAGATTATAGATAAATTGAAGGGTAGGTTGAGAGATGATGCATATAACAGTGATTTTCTGTTTGAGGTGAAGGAAAATTGGATGCTACAAGGTTGGAAGGGTAGAATTCTATTTGGTTCTTCTTGCTGGGTTCCAGCATAG
Protein sequence

>MS.gene31134.t1

MDKRVVGPHHEQSKSEFTSTVLGFINKFLMEDDDIEEDYNMFQHNEHEESSNALGYINQFLMEEDFEKEYTMFQDSYALQLTEKSFHDVISHPPPTAATAAAAATTLPSSSSSSSSYSSSSSSSSSSSSSSSSSSIQHQQNYNYVHSPSFSDYSFSSGSTSSYELDPIIDSYNYNNNAFLLPTTLPFPPNHIFSQSNPTLFPSFNNALSHEVIKIENFEEEHFLNVSKQVNVDDNSELSELFDKLLLGTKVAKGPLQNTSFQQNEELSNRFGGFRRKRSYEEVVDFRTLLMLCAQSISCNDISNANQILNQIKEHSYPTGDGTQRLAHFYGNALEARLAGTGSLIYRALSSKKKSAADMARAYQVYSSACPFEKLAIMFSNNAIFNVAKETESLHIIDFGVGYGFKWPAFIHRLSKRSGGPPKLKITGIDLPNSMERVNEAGLRLASYCKRFNVPFEFNCIAKNWESIKVEDFKIRKNEFVAVNCLFKFENLLDETVVSENPKGAVLDLIRKTNPNIFIHSIVNGGYDAPFFVTRFKEAVFHYSALFDTLDNNNSEREDPNRLMFEEVFWGKDIMNVIACEGCDRVERPETYRHWHSRHVGNGFKSLKLDKQIIDKLKGRLRDDAYNSDFLFEVKENWMLQGWKGRILFGSSCWVPA