AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene31267

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31267.t1 MTR_6g471120 100.000 168 0 0 1 168 1 168 5.53e-122 340
MS.gene31267.t1 MTR_2g094720 99.405 168 1 0 1 168 1 168 1.85e-121 339
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31267.t1 AT5G03500 77.976 168 37 0 1 168 11 178 1.60e-89 259
MS.gene31267.t1 AT5G03500 77.976 168 37 0 1 168 1 168 1.60e-89 259
MS.gene31267.t1 AT5G03500 77.976 168 37 0 1 168 1 168 1.60e-89 259
MS.gene31267.t1 AT5G03500 77.976 168 37 0 1 168 1 168 1.60e-89 259
MS.gene31267.t1 AT5G03500 77.976 168 37 0 1 168 1 168 1.60e-89 259
MS.gene31267.t1 AT5G03500 77.976 168 37 0 1 168 1 168 1.60e-89 259
MS.gene31267.t1 AT5G03500 77.976 168 37 0 1 168 1 168 1.60e-89 259
MS.gene31267.t1 AT5G03220 79.167 168 35 0 1 168 1 168 1.68e-84 246

Find 41 sgRNAs with CRISPR-Local

Find 250 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCAACTCTGATTCACATTT+TGG 0.181104 2.4:-7667633 MS.gene31267:CDS
AGAATTGCAACTACACATTT+TGG 0.197843 2.4:-7667885 MS.gene31267:CDS
CACCACCACCACCTTATTAC+AGG 0.297273 2.4:-7670373 MS.gene31267:CDS
AAGGAGCTGAGGTCACTTAA+TGG 0.305351 2.4:-7667908 MS.gene31267:CDS
TAAGATAGCCTACCAATATT+AGG 0.320366 2.4:+7668479 None:intergenic
GAAGGAACTTATATCTGTTT+CGG 0.341977 2.4:-7670293 MS.gene31267:CDS
GAATTCTCTGCGTCCTCATC+AGG 0.376395 2.4:-7667771 MS.gene31267:intron
CAGATATAAGTTCCTTCAAT+TGG 0.376411 2.4:+7670299 None:intergenic
TCTTTGTAAAGCCTGTAATA+AGG 0.393712 2.4:+7670362 None:intergenic
AAGCTGTGGAGGACATAAAT+AGG 0.396665 2.4:-7667586 MS.gene31267:intron
GAGTCATTGGCAACACTTGA+TGG 0.406036 2.4:-7666449 MS.gene31267:CDS
TATTCTAAAGGACCTAATAT+TGG 0.416061 2.4:-7668491 MS.gene31267:intron
CTGTAATAAGGTGGTGGTGG+TGG 0.437759 2.4:+7670374 None:intergenic
ACCCCTTGTTCTTCCAAGCT+TGG 0.438251 2.4:+7668524 None:intergenic
GGGGCTCTGGAGCAGACTCA+GGG 0.449600 2.4:+7670327 None:intergenic
GTGTTGCCAATGACTCGTTA+AGG 0.463201 2.4:+7666456 None:intergenic
GTTCCTTCAATTGGTGGTGG+GGG 0.472687 2.4:+7670308 None:intergenic
AAGAATCCTTAACGAGTCAT+TGG 0.477983 2.4:-7666462 MS.gene31267:CDS
AGTTCCTTCAATTGGTGGTG+GGG 0.479986 2.4:+7670307 None:intergenic
TAAGTTCCTTCAATTGGTGG+TGG 0.495504 2.4:+7670305 None:intergenic
TAGGTCCTTTAGAATAGAGT+TGG 0.518443 2.4:+7668498 None:intergenic
ACTCTCCTTGCATATTGTGA+TGG 0.520397 2.4:+7667836 None:intergenic
TAGTGATGTTCTACCAAGCT+TGG 0.528638 2.4:-7668537 MS.gene31267:CDS
CTACCAAGCTTGGAAGAACA+AGG 0.531498 2.4:-7668527 MS.gene31267:CDS
AAGTTCCTTCAATTGGTGGT+GGG 0.540469 2.4:+7670306 None:intergenic
GTACGCCAACTCTATTCTAA+AGG 0.542516 2.4:-7668503 MS.gene31267:CDS
TGTAATAAGGTGGTGGTGGT+GGG 0.546044 2.4:+7670375 None:intergenic
GAGGACGCAGAGAATTCAAA+AGG 0.547381 2.4:+7667777 None:intergenic
ATATAAGTTCCTTCAATTGG+TGG 0.550214 2.4:+7670302 None:intergenic
ACCTTCTATAGATTTCAAGA+AGG 0.564561 2.4:-7667927 MS.gene31267:intron
AGCCTGTAATAAGGTGGTGG+TGG 0.598331 2.4:+7670371 None:intergenic
AGAGCGTCGTAAACAAGCTG+TGG 0.612592 2.4:-7667600 MS.gene31267:CDS
ACCAAGCTTGGAAGAACAAG+GGG 0.614422 2.4:-7668525 MS.gene31267:CDS
AGAGACCATCACAATATGCA+AGG 0.622612 2.4:-7667841 MS.gene31267:CDS
GGAACTTATATCTGTTTCGG+AGG 0.626605 2.4:-7670290 MS.gene31267:CDS
AGAACATTGTATACCTGATG+AGG 0.629513 2.4:+7667758 None:intergenic
GCGTCGTAAACAAGCTGTGG+AGG 0.642228 2.4:-7667597 MS.gene31267:CDS
TTGTAAAGCCTGTAATAAGG+TGG 0.646875 2.4:+7670365 None:intergenic
TACCAAGCTTGGAAGAACAA+GGG 0.654458 2.4:-7668526 MS.gene31267:CDS
TAGATTTCAAGAAGGAGCTG+AGG 0.655101 2.4:-7667919 MS.gene31267:CDS
TAAAGCCTGTAATAAGGTGG+TGG 0.655388 2.4:+7670368 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATATTGTATTTACTTTAT+AGG - chr2.4:7669174-7669193 MS.gene31267:intron 10.0%
!!! TGATATTCATATTTTAATTA+TGG - chr2.4:7669723-7669742 MS.gene31267:intron 10.0%
!! AAGGAAAAAAAATAATGTTA+TGG + chr2.4:7667645-7667664 None:intergenic 15.0%
!! AATGTAGTTAAAATTGATAA+TGG - chr2.4:7669977-7669996 MS.gene31267:intron 15.0%
!! AGGAAAAAAAATAATGTTAT+GGG + chr2.4:7667644-7667663 None:intergenic 15.0%
!! ATAATAATATCAAAATTGCA+AGG + chr2.4:7666594-7666613 None:intergenic 15.0%
!! ATGAAACAAATTTCAATAAA+GGG + chr2.4:7667930-7667949 None:intergenic 15.0%
!! TAATAATATCAAAATTGCAA+GGG + chr2.4:7666593-7666612 None:intergenic 15.0%
!! TATAATGAAAATTTCAGAAA+CGG + chr2.4:7669157-7669176 None:intergenic 15.0%
!! TTAATAATGCCTAAAAAATT+GGG + chr2.4:7668802-7668821 None:intergenic 15.0%
!!! AAAAAGTACAATCTATATAA+AGG + chr2.4:7669756-7669775 None:intergenic 15.0%
!!! ATTCATATTTTAATTATGGA+AGG - chr2.4:7669727-7669746 MS.gene31267:intron 15.0%
!!! TTGCAATTTTGATATTATTA+TGG - chr2.4:7666593-7666612 MS.gene31267:intron 15.0%
!!! TTTAATTATGTGTCATTTAA+TGG - chr2.4:7666750-7666769 MS.gene31267:intron 15.0%
!!! TTTTATAAACTACTTAAATG+TGG + chr2.4:7667771-7667790 None:intergenic 15.0%
!!! TTTTTTTTTTTTTATTTCGC+TGG - chr2.4:7669293-7669312 MS.gene31267:intron 15.0%
!! AACATGTAGAAATAAGAATT+AGG + chr2.4:7669602-7669621 None:intergenic 20.0%
!! AAGTAGTTTATAAAAAGTAG+TGG - chr2.4:7667776-7667795 MS.gene31267:CDS 20.0%
!! AGGAATGATATCATTAAATA+GGG - chr2.4:7668003-7668022 MS.gene31267:intron 20.0%
!! AGTAGTTTATAAAAAGTAGT+GGG - chr2.4:7667777-7667796 MS.gene31267:CDS 20.0%
!! ATAATGAAAATTTCAGAAAC+GGG + chr2.4:7669156-7669175 None:intergenic 20.0%
!! ATGTATTAGAGAAATTACAA+AGG + chr2.4:7667664-7667683 None:intergenic 20.0%
!! ATTTGTTTCATGAGAATTAA+AGG - chr2.4:7667939-7667958 MS.gene31267:intron 20.0%
!! CATGAAACAAATTTCAATAA+AGG + chr2.4:7667931-7667950 None:intergenic 20.0%
!! GTTAATAATGCCTAAAAAAT+TGG + chr2.4:7668803-7668822 None:intergenic 20.0%
!! TAAACTCTAAATTGATTGAA+GGG - chr2.4:7667974-7667993 MS.gene31267:intron 20.0%
!! TAATTACAATGTTTATCAGA+AGG - chr2.4:7667296-7667315 MS.gene31267:intron 20.0%
!! TAGGAATGATATCATTAAAT+AGG - chr2.4:7668002-7668021 MS.gene31267:intron 20.0%
!! TGTTGTTGTATATTGAAATT+TGG - chr2.4:7668602-7668621 MS.gene31267:intron 20.0%
!! TTAAACTCTAAATTGATTGA+AGG - chr2.4:7667973-7667992 MS.gene31267:intron 20.0%
!!! AAATGAACTCATTTTCTTAA+AGG + chr2.4:7667862-7667881 None:intergenic 20.0%
!!! AACACGTAAACAAATATTTT+TGG + chr2.4:7668199-7668218 None:intergenic 20.0%
!!! AATGAACTCATTTTCTTAAA+GGG + chr2.4:7667861-7667880 None:intergenic 20.0%
!!! AATTAGTTTAACATAGCTTA+TGG + chr2.4:7667149-7667168 None:intergenic 20.0%
!!! ATATTATAACAGCTTTTGAA+AGG + chr2.4:7667442-7667461 None:intergenic 20.0%
!!! ATTGTAATTAAGTGTTTCTA+GGG + chr2.4:7667286-7667305 None:intergenic 20.0%
!!! TAATTGTTTTATGATACGTA+TGG - chr2.4:7670273-7670292 MS.gene31267:intron 20.0%
!!! TATTATAACAGCTTTTGAAA+GGG + chr2.4:7667441-7667460 None:intergenic 20.0%
!!! TTAGTTCATTCTTTTGAAAA+TGG - chr2.4:7669393-7669412 MS.gene31267:intron 20.0%
!!! TTATGGTTCTAATTACTTTA+TGG - chr2.4:7666610-7666629 MS.gene31267:intron 20.0%
!!! TTCTTTTGATTAACATCATT+TGG - chr2.4:7668688-7668707 MS.gene31267:intron 20.0%
!!! TTTGGTATGTAATTTTTTTG+TGG - chr2.4:7667499-7667518 MS.gene31267:intron 20.0%
! AAACAACCTTAACTTATTCA+GGG - chr2.4:7669465-7669484 MS.gene31267:intron 25.0%
! AATAAGTTAAGGTTGTTTAC+TGG + chr2.4:7669463-7669482 None:intergenic 25.0%
! AATTTCAATAAAGGGTAGTT+TGG + chr2.4:7667922-7667941 None:intergenic 25.0%
! ATAATATTGTCGTCTAAACA+TGG - chr2.4:7667455-7667474 MS.gene31267:intron 25.0%
! ATATAAACTTGCCTTTGATT+TGG - chr2.4:7669113-7669132 MS.gene31267:intron 25.0%
! ATCAGATTAAGTTCAGTTTA+GGG - chr2.4:7668094-7668113 MS.gene31267:intron 25.0%
! CAACTTCAATTATTTGGTTA+AGG - chr2.4:7667412-7667431 MS.gene31267:intron 25.0%
! CACTATATATATATACACTC+CGG - chr2.4:7667813-7667832 MS.gene31267:CDS 25.0%
! CATTCAATGCTTAATGTATT+TGG + chr2.4:7666864-7666883 None:intergenic 25.0%
! CATTGTAATTAAGTGTTTCT+AGG + chr2.4:7667287-7667306 None:intergenic 25.0%
! CTATTTACTATCTAGTTGTT+TGG - chr2.4:7667481-7667500 MS.gene31267:intron 25.0%
! GTAGAAATAAGAATTAGGAT+AGG + chr2.4:7669597-7669616 None:intergenic 25.0%
! TAAACAACCTTAACTTATTC+AGG - chr2.4:7669464-7669483 MS.gene31267:intron 25.0%
! TAATATTGTCGTCTAAACAT+GGG - chr2.4:7667456-7667475 MS.gene31267:intron 25.0%
! TCATGAGAATTAAAGGTTAT+TGG - chr2.4:7667946-7667965 MS.gene31267:intron 25.0%
! TTGAAAGATAAGCTCAAAAT+AGG + chr2.4:7667087-7667106 None:intergenic 25.0%
!! ATAATGTTATGGGATACTTT+TGG + chr2.4:7667634-7667653 None:intergenic 25.0%
!! ATTTTGCTTATAAATGTGAC+CGG + chr2.4:7666792-7666811 None:intergenic 25.0%
!! GTTAAGATTTGCGTATATTT+TGG - chr2.4:7668033-7668052 MS.gene31267:intron 25.0%
!! TAACTTATGTTCTAGCTATT+AGG + chr2.4:7669667-7669686 None:intergenic 25.0%
!! TAATGTTATGGGATACTTTT+GGG + chr2.4:7667633-7667652 None:intergenic 25.0%
!! TATTCTAAAGGACCTAATAT+TGG - chr2.4:7668343-7668362 MS.gene31267:intron 25.0%
!! TTATTGAAAGAGTTGATTTC+TGG - chr2.4:7669861-7669880 MS.gene31267:intron 25.0%
!! TTTGTAGTGTTGTTAAATAC+CGG - chr2.4:7668492-7668511 MS.gene31267:CDS 25.0%
!!! AATTTTTTTGTGGTTGTGAT+TGG - chr2.4:7667509-7667528 MS.gene31267:intron 25.0%
!!! ATAAGCAAAATGCACTTTTT+AGG - chr2.4:7666801-7666820 MS.gene31267:intron 25.0%
!!! TCCTCTTGTATTTCTATTTT+GGG + chr2.4:7669510-7669529 None:intergenic 25.0%
!!! TTTCCATTTTTGAAGTCTTT+GGG + chr2.4:7667550-7667569 None:intergenic 25.0%
!!! TTTTCCATTTTTGAAGTCTT+TGG + chr2.4:7667551-7667570 None:intergenic 25.0%
!!! TTTTTACCTGATTTTAGCTT+TGG - chr2.4:7666934-7666953 MS.gene31267:intron 25.0%
AAAATGTACAAAGATACACC+AGG + chr2.4:7670086-7670105 None:intergenic 30.0%
AAATGACTGTAATAGTGCTT+TGG - chr2.4:7670124-7670143 MS.gene31267:intron 30.0%
ACCTTCTATAGATTTCAAGA+AGG - chr2.4:7668907-7668926 MS.gene31267:intron 30.0%
ACTATAGACAGTCACATATT+AGG + chr2.4:7668766-7668785 None:intergenic 30.0%
AGAACCTTCTACAAATTTGT+TGG - chr2.4:7669326-7669345 MS.gene31267:intron 30.0%
ATATAAGTTCCTTCAATTGG+TGG + chr2.4:7666535-7666554 None:intergenic 30.0%
CAAAAGAATGAACTAACTTG+TGG + chr2.4:7669389-7669408 None:intergenic 30.0%
CAATGCACATTTGATATTAC+CGG - chr2.4:7667592-7667611 MS.gene31267:CDS 30.0%
CAGATATAAGTTCCTTCAAT+TGG + chr2.4:7666538-7666557 None:intergenic 30.0%
CATACCAACAAATTTGTAGA+AGG + chr2.4:7669333-7669352 None:intergenic 30.0%
CTGAAAAGAAAAGAGAAAGT+AGG + chr2.4:7670334-7670353 None:intergenic 30.0%
GAAGGAACTTATATCTGTTT+CGG - chr2.4:7666541-7666560 MS.gene31267:intron 30.0%
GATCAGATTAAGTTCAGTTT+AGG - chr2.4:7668093-7668112 MS.gene31267:intron 30.0%
GGTATTTAACAACACTACAA+AGG + chr2.4:7668493-7668512 None:intergenic 30.0%
GTAAAAATAAATACTCCCTC+CGG + chr2.4:7666921-7666940 None:intergenic 30.0%
GTTAGTTCACCAAAATTGAT+AGG - chr2.4:7670180-7670199 MS.gene31267:intron 30.0%
TAAGATAGCCTACCAATATT+AGG + chr2.4:7668358-7668377 None:intergenic 30.0%
TAGAAAAAGTTTGACAGGTA+TGG + chr2.4:7670015-7670034 None:intergenic 30.0%
TCCATTAGAAAAAGTTTGAC+AGG + chr2.4:7670020-7670039 None:intergenic 30.0%
TCCCAAAATAGAAATACAAG+AGG - chr2.4:7669506-7669525 MS.gene31267:intron 30.0%
TCCTTCTTGAAATCTATAGA+AGG + chr2.4:7668911-7668930 None:intergenic 30.0%
TCTTTGTAAAGCCTGTAATA+AGG + chr2.4:7666475-7666494 None:intergenic 30.0%
TGCAAAACCTGAAGAATTTA+AGG - chr2.4:7669786-7669805 MS.gene31267:intron 30.0%
TTGTAAGTTGTAACCTTCAT+GGG - chr2.4:7669354-7669373 MS.gene31267:intron 30.0%
! AGAATTGCAACTACACATTT+TGG - chr2.4:7668949-7668968 MS.gene31267:intron 30.0%
! AGCTTATCTTTCAAAGTGTT+TGG - chr2.4:7667093-7667112 MS.gene31267:intron 30.0%
! ATTTGGACAAACCACTATTT+TGG - chr2.4:7668619-7668638 MS.gene31267:intron 30.0%
! CACTTTTCCATTTTAAACTG+TGG - chr2.4:7669925-7669944 MS.gene31267:intron 30.0%
! CTACTTTCTCTTTTCTTTTC+AGG - chr2.4:7670332-7670351 MS.gene31267:CDS 30.0%
! CTTGATAGTTAATGCTGAAT+TGG - chr2.4:7668822-7668841 MS.gene31267:intron 30.0%
! CTTTTGCTTATAAATGTGAC+CGG - chr2.4:7666899-7666918 MS.gene31267:intron 30.0%
! GCTTATCTTTCAAAGTGTTT+GGG - chr2.4:7667094-7667113 MS.gene31267:intron 30.0%
! GTGATGCAAATTTTTGAACA+CGG + chr2.4:7669038-7669057 None:intergenic 30.0%
! TAATACATCTTCGACAGTTT+TGG - chr2.4:7667676-7667695 MS.gene31267:intron 30.0%
! TTTCCATTTTAAACTGTGGA+TGG - chr2.4:7669929-7669948 MS.gene31267:intron 30.0%
! TTTGGACAAACCACTATTTT+GGG - chr2.4:7668620-7668639 MS.gene31267:intron 30.0%
!! ACCTGTCAAACTTTTTCTAA+TGG - chr2.4:7670016-7670035 MS.gene31267:intron 30.0%
!! ATTAAGTGTTTCTAGGGTAA+AGG + chr2.4:7667280-7667299 None:intergenic 30.0%
!! CTCCTCTTGTATTTCTATTT+TGG + chr2.4:7669511-7669530 None:intergenic 30.0%
!! TTAAGTGTTTCTAGGGTAAA+GGG + chr2.4:7667279-7667298 None:intergenic 30.0%
!!! AATTGATTGAAGGGCATTTT+AGG - chr2.4:7667983-7668002 MS.gene31267:intron 30.0%
AAGAATCCTTAACGAGTCAT+TGG - chr2.4:7670372-7670391 MS.gene31267:CDS 35.0%
AAGCCCAAAGACTTCAAAAA+TGG - chr2.4:7667544-7667563 MS.gene31267:intron 35.0%
AGAAATCATAGCATCATACC+TGG - chr2.4:7670065-7670084 MS.gene31267:intron 35.0%
AGAACATTGTATACCTGATG+AGG + chr2.4:7669079-7669098 None:intergenic 35.0%
AGCTTATGACAACAGCTTAT+AGG - chr2.4:7667188-7667207 MS.gene31267:intron 35.0%
AGGTATGATGCTATGATTTC+TGG + chr2.4:7670066-7670085 None:intergenic 35.0%
ATGAGTTGTTACAGATAGTG+TGG - chr2.4:7668436-7668455 MS.gene31267:intron 35.0%
ATTACACAATGCTATAGCAC+CGG + chr2.4:7668514-7668533 None:intergenic 35.0%
CACATTTGATATTACCGGAT+AGG - chr2.4:7667597-7667616 MS.gene31267:CDS 35.0%
CATGAAAATGTTCCAAGAGT+GGG + chr2.4:7669627-7669646 None:intergenic 35.0%
CATTGGTATTAGATGCATGT+TGG + chr2.4:7667577-7667596 None:intergenic 35.0%
CGGTAATATCAAATGTGCAT+TGG + chr2.4:7667594-7667613 None:intergenic 35.0%
CTAAAGGACCTAATATTGGT+AGG - chr2.4:7668347-7668366 MS.gene31267:intron 35.0%
CTTCCATCCACAGTTTAAAA+TGG + chr2.4:7669935-7669954 None:intergenic 35.0%
CTTGTAAGTTGTAACCTTCA+TGG - chr2.4:7669353-7669372 MS.gene31267:intron 35.0%
TGACTTCCCTGAATAAGTTA+AGG + chr2.4:7669474-7669493 None:intergenic 35.0%
TGAGTTGTTACAGATAGTGT+GGG - chr2.4:7668437-7668456 MS.gene31267:intron 35.0%
TGCTAGTTAGTTGTTGTATC+TGG + chr2.4:7669570-7669589 None:intergenic 35.0%
TGGCAGCAACTTCAATTATT+TGG - chr2.4:7667406-7667425 MS.gene31267:intron 35.0%
TTGTAAAGCCTGTAATAAGG+TGG + chr2.4:7666472-7666491 None:intergenic 35.0%
TTGTCAAATAGCTGCTATAG+CGG - chr2.4:7668571-7668590 MS.gene31267:intron 35.0%
! AAAAAAAAAGTACGCAGCCA+TGG + chr2.4:7669840-7669859 None:intergenic 35.0%
! AAAAAAAAGTACGCAGCCAT+GGG + chr2.4:7669839-7669858 None:intergenic 35.0%
! AACCTGAAGAATTTAAGGCT+TGG - chr2.4:7669791-7669810 MS.gene31267:intron 35.0%
! ACAGCAAAACAATCCAGTTT+TGG + chr2.4:7668063-7668082 None:intergenic 35.0%
! AGCAACTCTGATTCACATTT+TGG - chr2.4:7669201-7669220 MS.gene31267:intron 35.0%
! ATGGTTGCTCCTATCAATTT+TGG + chr2.4:7670192-7670211 None:intergenic 35.0%
! ATTTTATGCAGAAGCCTTTC+TGG - chr2.4:7668223-7668242 MS.gene31267:intron 35.0%
! CACTCTTGGAACATTTTCAT+GGG - chr2.4:7669626-7669645 MS.gene31267:intron 35.0%
! CATGACAACTTTGCTGTATT+TGG - chr2.4:7667045-7667064 MS.gene31267:intron 35.0%
! TATCTTTCAAAGTGTTTGGG+TGG - chr2.4:7667097-7667116 MS.gene31267:intron 35.0%
! TCTAAAAGCTTGGAGCTTTT+TGG - chr2.4:7666969-7666988 MS.gene31267:intron 35.0%
! TGACACTCGAATAGACATTT+TGG - chr2.4:7666696-7666715 MS.gene31267:intron 35.0%
! TGTGCGTTATTCTAAAAGCT+TGG - chr2.4:7666959-7666978 MS.gene31267:intron 35.0%
! TTATGTTCTAGCTATTAGGC+AGG + chr2.4:7669663-7669682 None:intergenic 35.0%
!! ATATTTTGGACCACCAAAAC+TGG - chr2.4:7668047-7668066 MS.gene31267:intron 35.0%
!! CTTTCTCTTTTCTTTTCAGG+AGG - chr2.4:7670335-7670354 MS.gene31267:CDS 35.0%
!! TACAAGAGAAGCATTGTCTT+TGG + chr2.4:7670245-7670264 None:intergenic 35.0%
!! TAGGTCCTTTAGAATAGAGT+TGG + chr2.4:7668339-7668358 None:intergenic 35.0%
!! TAGTGCTTTGGAAACTTGAT+TGG - chr2.4:7670136-7670155 MS.gene31267:intron 35.0%
!! TCGAATAGACATTTTGGAAG+AGG - chr2.4:7666702-7666721 MS.gene31267:intron 35.0%
!!! AGACTCTAACTTTTTCACAC+TGG - chr2.4:7669432-7669451 MS.gene31267:intron 35.0%
!!! GACTCTAACTTTTTCACACT+GGG - chr2.4:7669433-7669452 MS.gene31267:intron 35.0%
AAGCTGTGGAGGACATAAAT+AGG - chr2.4:7669248-7669267 MS.gene31267:intron 40.0%
ACAATATGATCACGCCAGAA+AGG + chr2.4:7668240-7668259 None:intergenic 40.0%
ACTCTCCTTGCATATTGTGA+TGG + chr2.4:7669001-7669020 None:intergenic 40.0%
AGAGACCATCACAATATGCA+AGG - chr2.4:7668993-7669012 MS.gene31267:intron 40.0%
ATGGCAAAACTGGGTAAAGA+AGG + chr2.4:7670220-7670239 None:intergenic 40.0%
CACATGCCAAAGCTAAAATC+AGG + chr2.4:7666943-7666962 None:intergenic 40.0%
CCATGAAAATGTTCCAAGAG+TGG + chr2.4:7669628-7669647 None:intergenic 40.0%
GACCAAGCCTTAAATTCTTC+AGG + chr2.4:7669796-7669815 None:intergenic 40.0%
GAGTTCTGTCTTGCAAAACA+GGG - chr2.4:7668146-7668165 MS.gene31267:intron 40.0%
GGAACTTATATCTGTTTCGG+AGG - chr2.4:7666544-7666563 MS.gene31267:intron 40.0%
GTACGCCAACTCTATTCTAA+AGG - chr2.4:7668331-7668350 MS.gene31267:intron 40.0%
GTGGATTTAGTAACCCATGA+AGG + chr2.4:7669370-7669389 None:intergenic 40.0%
TAAAGCCTGTAATAAGGTGG+TGG + chr2.4:7666469-7666488 None:intergenic 40.0%
TAAGTTCCTTCAATTGGTGG+TGG + chr2.4:7666532-7666551 None:intergenic 40.0%
TACCAAGCTTGGAAGAACAA+GGG - chr2.4:7668308-7668327 MS.gene31267:intron 40.0%
TAGATTTCAAGAAGGAGCTG+AGG - chr2.4:7668915-7668934 MS.gene31267:intron 40.0%
TAGTGATGTTCTACCAAGCT+TGG - chr2.4:7668297-7668316 MS.gene31267:intron 40.0%
TCAGTGAAGGTTGTTAGTGT+TGG + chr2.4:7669543-7669562 None:intergenic 40.0%
TCCAGAACATCCCAAAATAG+TGG + chr2.4:7668633-7668652 None:intergenic 40.0%
TGAGTTCTGTCTTGCAAAAC+AGG - chr2.4:7668145-7668164 MS.gene31267:intron 40.0%
TGCTTATAAATGTGACCGGA+GGG - chr2.4:7666903-7666922 MS.gene31267:intron 40.0%
TGCTTATAAATGTGACCGGA+GGG + chr2.4:7666922-7666903 None:intergenic 40.0%
TGGAAAATTAGTACTCCCTC+CGG - chr2.4:7666770-7666789 MS.gene31267:intron 40.0%
TGGCAAAACTGGGTAAAGAA+GGG + chr2.4:7670219-7670238 None:intergenic 40.0%
TGTATCTGGTGAATCAGTGA+AGG + chr2.4:7669556-7669575 None:intergenic 40.0%
TGTCTATAGTCTAGACCTGT+GGG - chr2.4:7668776-7668795 MS.gene31267:intron 40.0%
TTGCTTATAAATGTGACCGG+AGG - chr2.4:7666902-7666921 MS.gene31267:intron 40.0%
TTGCTTATAAATGTGACCGG+AGG + chr2.4:7666921-7666902 None:intergenic 40.0%
! AAGTTCCTTCAATTGGTGGT+GGG + chr2.4:7666531-7666550 None:intergenic 40.0%
! CCACTCTTGGAACATTTTCA+TGG - chr2.4:7669625-7669644 MS.gene31267:intron 40.0%
! CGAGTGTCATTGTAATAGCA+GGG + chr2.4:7666685-7666704 None:intergenic 40.0%
! TCGAGTGTCATTGTAATAGC+AGG + chr2.4:7666686-7666705 None:intergenic 40.0%
! TCTACATGTTTTCCCACTCT+TGG - chr2.4:7669612-7669631 MS.gene31267:intron 40.0%
! TTCTTTACCCAGTTTTGCCA+TGG - chr2.4:7670219-7670238 MS.gene31267:intron 40.0%
!! ACCAGAAGCATCCAAATCAA+AGG + chr2.4:7669127-7669146 None:intergenic 40.0%
!! GCAAAACAATCCAGTTTTGG+TGG + chr2.4:7668060-7668079 None:intergenic 40.0%
!! TACATCTTCGACAGTTTTGG+TGG - chr2.4:7667679-7667698 MS.gene31267:intron 40.0%
!!! ACCACTATTTTGGGATGTTC+TGG - chr2.4:7668629-7668648 MS.gene31267:intron 40.0%
!!! ATGGGATACTTTTGGGACTT+TGG + chr2.4:7667626-7667645 None:intergenic 40.0%
!!! TGGGATACTTTTGGGACTTT+GGG + chr2.4:7667625-7667644 None:intergenic 40.0%
AAGGAGCTGAGGTCACTTAA+TGG - chr2.4:7668926-7668945 MS.gene31267:intron 45.0%
ACAGAACTCAACCTACCTCA+AGG + chr2.4:7668135-7668154 None:intergenic 45.0%
ACCAAGCTTGGAAGAACAAG+GGG - chr2.4:7668309-7668328 MS.gene31267:intron 45.0%
ACTCAAATGAGTGACCTCCT+AGG - chr2.4:7667379-7667398 MS.gene31267:intron 45.0%
ATGAGTCAAACCTGCCATGA+TGG + chr2.4:7667344-7667363 None:intergenic 45.0%
ATGGGTGTACGAACCTATGA+GGG + chr2.4:7669821-7669840 None:intergenic 45.0%
CTACCAAGCTTGGAAGAACA+AGG - chr2.4:7668307-7668326 MS.gene31267:intron 45.0%
CTGGGTAAAGAAGGGAGAAA+TGG + chr2.4:7670211-7670230 None:intergenic 45.0%
CTGTCTATAGTCTAGACCTG+TGG - chr2.4:7668775-7668794 MS.gene31267:intron 45.0%
GAGGACGCAGAGAATTCAAA+AGG + chr2.4:7669060-7669079 None:intergenic 45.0%
GAGTCATTGGCAACACTTGA+TGG - chr2.4:7670385-7670404 MS.gene31267:CDS 45.0%
GTGTTGCCAATGACTCGTTA+AGG + chr2.4:7670381-7670400 None:intergenic 45.0%
TCAACCTACCTCAAGGTATG+TGG + chr2.4:7668128-7668147 None:intergenic 45.0%
TCATCCAACCACATACCTTG+AGG - chr2.4:7668117-7668136 MS.gene31267:intron 45.0%
TCTTTGGCCATGGCAAAACT+GGG + chr2.4:7670229-7670248 None:intergenic 45.0%
TGAGTCAAACCTGCCATGAT+GGG + chr2.4:7667343-7667362 None:intergenic 45.0%
!! AGAAGCATTGTCTTTGGCCA+TGG + chr2.4:7670239-7670258 None:intergenic 45.0%
!! AGTTCCTTCAATTGGTGGTG+GGG + chr2.4:7666530-7666549 None:intergenic 45.0%
!! GCCTTTGATTTGGATGCTTC+TGG - chr2.4:7669123-7669142 MS.gene31267:intron 45.0%
!! GCTAGTCTTGTATTCGAGAC+CGG + chr2.4:7667835-7667854 None:intergenic 45.0%
!! GTTTTGGTGGAGACAAGGAT+CGG - chr2.4:7667692-7667711 MS.gene31267:intron 45.0%
!! TGTAATAAGGTGGTGGTGGT+GGG + chr2.4:7666462-7666481 None:intergenic 45.0%
!!! ACCTGTGGGCCCAATTTTTT+AGG - chr2.4:7668790-7668809 MS.gene31267:intron 45.0%
!! ATAAAAAAAAAAAAAAAATC+AGG + chr2.4:7669288-7669307 None:intergenic 5.0%
ACCCCTTGTTCTTCCAAGCT+TGG + chr2.4:7668313-7668332 None:intergenic 50.0%
CACCACCACCACCTTATTAC+AGG - chr2.4:7666461-7666480 MS.gene31267:CDS 50.0%
CATGGCAGGTTTGACTCATG+AGG - chr2.4:7667345-7667364 MS.gene31267:intron 50.0%
CATGGGTGTACGAACCTATG+AGG + chr2.4:7669822-7669841 None:intergenic 50.0%
CCAACCACATACCTTGAGGT+AGG - chr2.4:7668121-7668140 MS.gene31267:intron 50.0%
CCTACCTCAAGGTATGTGGT+TGG + chr2.4:7668124-7668143 None:intergenic 50.0%
CTCATAGGTTCGTACACCCA+TGG - chr2.4:7669820-7669839 MS.gene31267:intron 50.0%
GAAGTTGCTGCCATATGCCT+AGG + chr2.4:7667399-7667418 None:intergenic 50.0%
GAATTCTCTGCGTCCTCATC+AGG - chr2.4:7669063-7669082 MS.gene31267:intron 50.0%
GCCTAAAAAATTGGGCCCAC+AGG + chr2.4:7668794-7668813 None:intergenic 50.0%
GCTAGAGAAAGCTCCCATCA+TGG - chr2.4:7667327-7667346 MS.gene31267:intron 50.0%
GTCTTTGGCCATGGCAAAAC+TGG + chr2.4:7670230-7670249 None:intergenic 50.0%
TGAGTGACCTCCTAGGCATA+TGG - chr2.4:7667386-7667405 MS.gene31267:intron 50.0%
TGGGACTTTGGGTACCTATC+CGG + chr2.4:7667614-7667633 None:intergenic 50.0%
TGGGAGCTTTCTCTAGCACA+AGG + chr2.4:7667324-7667343 None:intergenic 50.0%
! AGCCTGTAATAAGGTGGTGG+TGG + chr2.4:7666466-7666485 None:intergenic 50.0%
!! AGAGCGTCGTAAACAAGCTG+TGG - chr2.4:7669234-7669253 MS.gene31267:intron 50.0%
!! CGACAGTTTTGGTGGAGACA+AGG - chr2.4:7667687-7667706 MS.gene31267:intron 50.0%
!! CTGTAATAAGGTGGTGGTGG+TGG + chr2.4:7666463-7666482 None:intergenic 50.0%
!! GTTCCTTCAATTGGTGGTGG+GGG + chr2.4:7666529-7666548 None:intergenic 50.0%
GAGAAAGCTCCCATCATGGC+AGG - chr2.4:7667331-7667350 MS.gene31267:intron 55.0%
GCGTCGTAAACAAGCTGTGG+AGG - chr2.4:7669237-7669256 MS.gene31267:intron 55.0%
GGTGTACGAACCTATGAGGG+AGG + chr2.4:7669818-7669837 None:intergenic 55.0%
GTTGCTGCCATATGCCTAGG+AGG + chr2.4:7667396-7667415 None:intergenic 55.0%
TCGGAGGCAGTTACACTGTG+AGG - chr2.4:7666560-7666579 MS.gene31267:intron 55.0%
! AAGGCTTGGTCCTCCCTCAT+AGG - chr2.4:7669805-7669824 MS.gene31267:intron 55.0%
GAGCCCCCACCACCAATTGA+AGG - chr2.4:7666523-7666542 MS.gene31267:intron 60.0%
!! TCAATTGGTGGTGGGGGCTC+TGG + chr2.4:7666523-7666542 None:intergenic 60.0%
!! GGGGCTCTGGAGCAGACTCA+GGG + chr2.4:7666510-7666529 None:intergenic 65.0%
!! GGGGGCTCTGGAGCAGACTC+AGG + chr2.4:7666511-7666530 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 7666442 7670414 7666442 ID=MS.gene31267
chr2.4 mRNA 7666442 7670414 7666442 ID=MS.gene31267.t1;Parent=MS.gene31267
chr2.4 exon 7670280 7670414 7670280 ID=MS.gene31267.t1.exon1;Parent=MS.gene31267.t1
chr2.4 CDS 7670280 7670414 7670280 ID=cds.MS.gene31267.t1;Parent=MS.gene31267.t1
chr2.4 exon 7668492 7668561 7668492 ID=MS.gene31267.t1.exon2;Parent=MS.gene31267.t1
chr2.4 CDS 7668492 7668561 7668492 ID=cds.MS.gene31267.t1;Parent=MS.gene31267.t1
chr2.4 exon 7667772 7667938 7667772 ID=MS.gene31267.t1.exon3;Parent=MS.gene31267.t1
chr2.4 CDS 7667772 7667938 7667772 ID=cds.MS.gene31267.t1;Parent=MS.gene31267.t1
chr2.4 exon 7667587 7667660 7667587 ID=MS.gene31267.t1.exon4;Parent=MS.gene31267.t1
chr2.4 CDS 7667587 7667660 7667587 ID=cds.MS.gene31267.t1;Parent=MS.gene31267.t1
chr2.4 exon 7666442 7666502 7666442 ID=MS.gene31267.t1.exon5;Parent=MS.gene31267.t1
chr2.4 CDS 7666442 7666502 7666442 ID=cds.MS.gene31267.t1;Parent=MS.gene31267.t1
Gene Sequence

>MS.gene31267

ATGGCAACAGCAACATACCCACCACCACCACCTTATTACAGGCTTTACAAAGATTATGTGCAAGACCCTGAGTCTGCTCCAGAGCCCCCACCACCAATTGAAGGAACTTATATCTGTTTCGGAGGCAGTTACACTGTGAGGTTTTTTACCCTTGCAATTTTGATATTATTATGGTTCTAATTACTTTATGGATAAATAGTTTAAATAGCTATTTATTCGAGACAATGTTGCTAAGATAAGCCCTGCTATTACAATGACACTCGAATAGACATTTTGGAAGAGGAACTTGTGCAGAATGAAAAGTTAGTTTTAATTATGTGTCATTTAATGGAAAATTAGTACTCCCTCCGGTCACATTTATAAGCAAAATGCACTTTTTAGGTTCATTTAATAAATAATGTATTTGATCTATATATTGACCAAATACATTAAGCATTGAATGAATCTAAAAAGTGTTCTTTTGCTTATAAATGTGACCGGAGGGAGTATTTATTTTTACCTGATTTTAGCTTTGGCATGTGCGTTATTCTAAAAGCTTGGAGCTTTTTGGTATATAAAGAAATCGAAAAGTGCTTTTATTTGCTTCAAAGTTGTTTTAATGAACATGACAACTTTGCTGTATTTGGATTGACTTATGAAAATCCTATTTTGAGCTTATCTTTCAAAGTGTTTGGGTGGTCTTAAATAAACAACTTATGACATATCCATAAGCTATGTTAAACTAATTTCAATAAGCTCTCTAGAATAGCTTATGACAACAGCTTATAGGACAACAGTTTCACTATGTTTTGTTTGATCATAGAAATAATTTATGCGTCAGTGCTTATTTAATAACCCTTTACCCTAGAAACACTTAATTACAATGTTTATCAGAAGGAGCCTTGTGCTAGAGAAAGCTCCCATCATGGCAGGTTTGACTCATGAGGTTGATTTCACGACTCAAATGAGTGACCTCCTAGGCATATGGCAGCAACTTCAATTATTTGGTTAAGGTTTCCCTTTCAAAAGCTGTTATAATATTGTCGTCTAAACATGGGTTCTATTTACTATCTAGTTGTTTGGTATGTAATTTTTTTGTGGTTGTGATTGGACGTCATCTATGAAGCCCAAAGACTTCAAAAATGGAAAAGTACCAACATGCATCTAATACCAATGCACATTTGATATTACCGGATAGGTACCCAAAGTCCCAAAAGTATCCCATAACATTATTTTTTTTCCTTTGTAATTTCTCTAATACATCTTCGACAGTTTTGGTGGAGACAAGGATCGGTCATTAATTGTGACGAGAGAAAAATTAAATTTTAAAAGCAAATTACAATAAAACCACATTTAAGTAGTTTATAAAAAGTAGTGGGAGTACATCAGCAACACTATATATATATACACTCCGGTCTCGAATACAAGACTAGCTCACCCTTTAAGAAAATGAGTTCATTTAGTCTTCTTGATCTAATAAAAAAGATTAATACGTTCTCCAAACTACCCTTTATTGAAATTTGTTTCATGAGAATTAAAGGTTATTGGAATATTAAACTCTAAATTGATTGAAGGGCATTTTAGGAATGATATCATTAAATAGGGTAATGTAGTTAAGATTTGCGTATATTTTGGACCACCAAAACTGGATTGTTTTGCTGTTTATATTCTTGATCAGATTAAGTTCAGTTTAGGGTCATCCAACCACATACCTTGAGGTAGGTTGAGTTCTGTCTTGCAAAACAGGGTTTCTTATTAGTTTGTGATGTAATTTTCCAAAAATATTTGTTTACGTGTTTGAGATTTTATGCAGAAGCCTTTCTGGCGTGATCATATTGTGTTAATATTTTGTTTCTTTATTTATTTTTACACAGACTAGTGATGTTCTACCAAGCTTGGAAGAACAAGGGGTACGCCAACTCTATTCTAAAGGACCTAATATTGGTAGGCTATCTTACGCTTTTTTGCTTTTTTTTTATGCTCAGTTAAATTTGAAAATGTTGTGAAGTGACTGTATGAGTTGTTACAGATAGTGTGGGTTTTAATTATATTATGTTTTTCAGTATTATCCTTTGTAGTGTTGTTAAATACCGGTGCTATAGCATTGTGTAATTTGAACAAATCACTATTGTTCAATGATAAAGAAAGTGTTGTCAAATAGCTGCTATAGCGGCACTACACTGTTGTTGTATATTGAAATTTGGACAAACCACTATTTTGGGATGTTCTGGATCACAATCTTTTCACTTTTTTGTCTATATAAATGTTTCTTTTGATTAACATCATTTGGTTTATGTTTCACATTTTTCATTTAGTGATTCTTGATATAATAAACATTGTAACCTAATATGTGACTGTCTATAGTCTAGACCTGTGGGCCCAATTTTTTAGGCATTATTAACTTGATAGTTAATGCTGAATTGGTAACAAAAGTCAATGCATAGTGTCTCTACTATATAACTTATTACTCGTTATAGTAACATCTTACCTTCTATAGATTTCAAGAAGGAGCTGAGGTCACTTAATGGAGAATTGCAACTACACATTTTGGAGCTTGCTGATATTCTTATTGAGAGACCATCACAATATGCAAGGAGAGTCGAAGAAATTTCAACCGTGTTCAAAAATTTGCATCACCTTTTGAATTCTCTGCGTCCTCATCAGGTATACAATGTTCTAGACAGCTATAAGTATATAAACTTGCCTTTGATTTGGATGCTTCTGGTCTTTTTCCCGTTTCTGAAATTTTCATTATATATTGTATTTACTTTATAGGCAAGAGCAACTCTGATTCACATTTTGGAACTTCAGATAGAGCGTCGTAAACAAGCTGTGGAGGACATAAATAGGTCTGCAATACATTTCCTGATTTTTTTTTTTTTTTTATTTCGCTGGCTATGCTTTCAGAACCTTCTACAAATTTGTTGGTATGCTTGTAAGTTGTAACCTTCATGGGTTACTAAATCCACAAGTTAGTTCATTCTTTTGAAAATGGTGAAAGTTAACACAAGAGACTCTAACTTTTTCACACTGGGTAAACCAGTAAACAACCTTAACTTATTCAGGGAAGTCATATTTATTGATCTCCCAAAATAGAAATACAAGAGGAGATAAAATAACCAACACTAACAACCTTCACTGATTCACCAGATACAACAACTAACTAGCACAAGCCTATCCTAATTCTTATTTCTACATGTTTTCCCACTCTTGGAACATTTTCATGGGACTAGTTACATCCTGCCTAATAGCTAGAACATAAGTTACTGATATAGAATACAGTTCATTATAAAGTCAAAGTTTGATATTCATATTTTAATTATGGAAGGCTTCCTTTATATAGATTGTACTTTTTTGATAACATGTGCAAAACCTGAAGAATTTAAGGCTTGGTCCTCCCTCATAGGTTCGTACACCCATGGCTGCGTACTTTTTTTTTTTTATTGAAAGAGTTGATTTCTGGAATCTAGTTACATGTTGAAATAAAATTTTATTTGCTATAGTCACTTTTCCATTTTAAACTGTGGATGGAAGATTATAAACTGCAATTTTTAATAATGTAGTTAAAATTGATAATGGAAACATCATCTGCCATACCTGTCAAACTTTTTCTAATGGAATACAATCATTTTACTTTGACAACCAGAAATCATAGCATCATACCTGGTGTATCTTTGTACATTTTGTTTGCTGTATACAAATAAAATGACTGTAATAGTGCTTTGGAAACTTGATTGGTCTAATTTATGAGATTGTATAGTTAGTTCACCAAAATTGATAGGAGCAACCATTTCTCCCTTCTTTACCCAGTTTTGCCATGGCCAAAGACAATGCTTCTCTTGTATATTATAGTAATTGTTTTATGATACGTATGGTACTTTGTTAAGCTCTTGTTACTTGTCTTTTTGAACCTACTTTCTCTTTTCTTTTCAGGAGGAGAGAAGAAGCTCGAAGAATCCTTAACGAGTCATTGGCAACACTTGATGGCCATTAA
Protein sequence

>MS.gene31267.t1

MATATYPPPPPYYRLYKDYVQDPESAPEPPPPIEGTYICFGGSYTTSDVLPSLEEQGVRQLYSKGPNIDFKKELRSLNGELQLHILELADILIERPSQYARRVEEISTVFKNLHHLLNSLRPHQARATLIHILELQIERRKQAVEDINRRREEARRILNESLATLDGH