AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene31700

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31700.t1 MTR_1g006650 94.767 344 14 2 1 340 1 344 0.0 650
MS.gene31700.t1 MTR_3g109760 55.247 324 116 8 19 340 22 318 1.65e-104 309
MS.gene31700.t1 MTR_8g013950 46.884 337 131 12 23 340 35 342 5.79e-78 242
MS.gene31700.t1 MTR_8g076340 47.317 205 90 5 96 300 102 288 1.74e-46 159
MS.gene31700.t1 MTR_4g071590 34.615 312 141 10 5 299 15 280 6.74e-43 150
MS.gene31700.t1 MTR_4g471590 34.615 312 141 10 5 299 15 280 6.74e-43 150
MS.gene31700.t1 MTR_6g032830 39.732 224 101 6 107 296 35 258 6.77e-41 144
MS.gene31700.t1 MTR_5g021340 44.156 231 95 7 94 300 150 370 3.74e-39 143
MS.gene31700.t1 MTR_1g094930 35.463 313 142 11 27 294 9 306 1.46e-37 137
MS.gene31700.t1 MTR_3g117380 37.209 215 117 3 87 300 102 299 3.05e-37 136
MS.gene31700.t1 MTR_1g046680 78.873 71 15 0 229 299 171 241 9.79e-35 127
MS.gene31700.t1 MTR_7g085410 39.370 254 110 10 73 294 11 252 1.02e-34 128
MS.gene31700.t1 MTR_5g007600 74.648 71 18 0 229 299 258 328 4.68e-33 125
MS.gene31700.t1 MTR_5g007600 39.234 209 94 4 101 300 43 227 5.88e-29 114
MS.gene31700.t1 MTR_1g094930 78.462 65 14 0 230 294 147 211 1.10e-32 122
MS.gene31700.t1 MTR_1g116010 55.963 109 43 2 209 316 142 246 6.31e-32 120
MS.gene31700.t1 MTR_2g094600 76.923 65 15 0 230 294 216 280 1.75e-31 120
MS.gene31700.t1 MTR_4g058910 73.134 67 18 0 230 296 314 380 2.93e-30 119
MS.gene31700.t1 MTR_7g446110 62.903 62 23 0 230 291 187 248 3.65e-21 91.3
MS.gene31700.t1 MTR_7g445950 32.124 193 90 6 99 291 30 181 3.31e-19 84.3
MS.gene31700.t1 MTR_7g446070 33.495 206 105 8 88 291 18 193 6.44e-19 84.0
MS.gene31700.t1 MTR_7g445980 62.712 59 21 1 234 291 37 95 1.47e-18 80.1
MS.gene31700.t1 MTR_7g112330 70.588 34 10 0 232 265 24 57 2.80e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31700.t1 AT5G25830 56.981 265 97 7 93 340 67 331 6.29e-92 278
MS.gene31700.t1 AT4G32890 43.881 335 131 9 21 340 16 308 5.30e-80 246
MS.gene31700.t1 AT3G60530 48.309 207 63 6 93 298 63 226 3.63e-49 165
MS.gene31700.t1 AT2G45050 43.277 238 79 7 89 310 60 257 8.41e-49 165
MS.gene31700.t1 AT5G66320 38.519 270 116 8 69 312 86 331 3.61e-39 142
MS.gene31700.t1 AT5G66320 38.519 270 116 8 69 312 86 331 3.61e-39 142
MS.gene31700.t1 AT4G36240 37.544 285 106 9 22 297 10 231 6.03e-39 138
MS.gene31700.t1 AT4G34680 38.710 217 82 5 90 299 77 249 1.39e-36 133
MS.gene31700.t1 AT4G34680 38.710 217 82 5 90 299 77 249 1.39e-36 133
MS.gene31700.t1 AT3G51080 34.304 309 157 10 6 298 11 289 3.10e-34 128
MS.gene31700.t1 AT3G24050 74.286 70 18 0 229 298 193 262 2.58e-33 124
MS.gene31700.t1 AT1G08010 65.476 84 23 1 225 302 215 298 3.74e-33 125
MS.gene31700.t1 AT1G08010 65.476 84 23 1 225 302 215 298 3.74e-33 125
MS.gene31700.t1 AT1G08010 65.476 84 23 1 225 302 215 298 3.74e-33 125
MS.gene31700.t1 AT1G08000 70.833 72 21 0 225 296 213 284 3.52e-31 120
MS.gene31700.t1 AT1G08000 70.833 72 21 0 225 296 213 284 3.52e-31 120
MS.gene31700.t1 AT3G54810 62.963 81 30 0 230 310 229 309 6.24e-31 119
MS.gene31700.t1 AT3G54810 62.963 81 30 0 230 310 229 309 6.24e-31 119
MS.gene31700.t1 AT2G28340 69.841 63 19 0 232 294 193 255 1.32e-26 107
MS.gene31700.t1 AT3G45170 64.179 67 24 0 232 298 117 183 2.63e-26 104

Find 117 sgRNAs with CRISPR-Local

Find 143 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCGTCACTGTCCTTTCTTCT+TGG 0.090798 1.1:+12527438 None:intergenic
CCAATATCAGGAACGACATT+AGG 0.287162 1.1:+12528305 None:intergenic
GCAGTCTTTGCCTGCTTCCT+TGG 0.291400 1.1:+12527462 None:intergenic
TACTCTGATGTTGTAAAATC+TGG 0.292416 1.1:+12527160 None:intergenic
TATATTCAACAAGGAAGTTT+TGG 0.298704 1.1:-12527628 MS.gene31700:CDS
CTAGCTGCAGGCCTGTATTC+AGG 0.333508 1.1:+12527282 None:intergenic
CTCCGGAGACCTGTGTGTTC+CGG 0.345236 1.1:-12528255 MS.gene31700:intron
CAAAAGATGCAACTGATATC+AGG 0.358334 1.1:-12527714 MS.gene31700:CDS
CCTAATGTCGTTCCTGATAT+TGG 0.361673 1.1:-12528305 MS.gene31700:CDS
AAAACTTCCTTGTTGAATAT+AGG 0.362486 1.1:+12527630 None:intergenic
GAAATAGGAGGATGCTTTAC+GGG 0.365602 1.1:-12527790 MS.gene31700:intron
CGACGTCATTGTTGAGGTTA+AGG 0.370723 1.1:+12528450 None:intergenic
TGTTGAATATAGGACTGTTT+CGG 0.382615 1.1:+12527640 None:intergenic
AAGACACCACAATGGCGAAC+CGG 0.386976 1.1:-12527369 MS.gene31700:CDS
ACGATGCGAGTTGGAGTGTT+TGG 0.387998 1.1:+12527242 None:intergenic
AAATTCTGACAGCCAATATC+AGG 0.389939 1.1:+12528293 None:intergenic
TGAAATAGGAGGATGCTTTA+CGG 0.392306 1.1:-12527791 MS.gene31700:intron
TTACTTGATTCACCAACATA+TGG 0.405233 1.1:-12527109 MS.gene31700:CDS
AGGACTGTTTCGGTTGGGTT+GGG 0.406165 1.1:+12527650 None:intergenic
GTTGGGTTGGGAAAGCTCAC+GGG 0.412320 1.1:+12527662 None:intergenic
CAATGGCGAACCGGGCCTAA+TGG 0.414512 1.1:-12527360 MS.gene31700:CDS
CACACAGGTCTCCGGAGAAA+TGG 0.416213 1.1:+12528261 None:intergenic
CACGGTAACGGTGGAGGAAG+AGG 0.434827 1.1:+12528367 None:intergenic
CAATATCAGGAACGACATTA+GGG 0.440367 1.1:+12528306 None:intergenic
CTCTTGCTACGGGCCTTAGC+TGG 0.442400 1.1:+12527603 None:intergenic
TGTCGGAGGAAAAGGTGGTT+TGG 0.443866 1.1:+12528507 None:intergenic
GGTTGGGTTGGGAAAGCTCA+CGG 0.448750 1.1:+12527661 None:intergenic
TACATCATCTGCCTAAAATC+AGG 0.450652 1.1:+12527084 None:intergenic
GCTCACGGGGTTCTTCGTCC+TGG 0.451765 1.1:+12527676 None:intergenic
AATATAGGACTGTTTCGGTT+GGG 0.459476 1.1:+12527645 None:intergenic
TAGGACTGTTTCGGTTGGGT+TGG 0.462649 1.1:+12527649 None:intergenic
AGTGTTGTTGTCGGAGGAAA+AGG 0.462952 1.1:+12528499 None:intergenic
AAGGACAGTGACGGAGGGTC+AGG 0.466229 1.1:-12527429 MS.gene31700:CDS
GAGGCAGAAGGAAATGTTGC+GGG 0.466463 1.1:-12527202 MS.gene31700:CDS
TACTTGATTCACCAACATAT+GGG 0.466490 1.1:-12527108 MS.gene31700:CDS
CAACATTGGAAAGCCATTCC+AGG 0.469957 1.1:+12527766 None:intergenic
GTCGATTGGTGCCTGAATAC+AGG 0.470023 1.1:-12527293 MS.gene31700:CDS
AGACACCACAATGGCGAACC+GGG 0.471491 1.1:-12527368 MS.gene31700:CDS
GCCCACGAGTCCTCTTGCTA+CGG 0.473059 1.1:+12527592 None:intergenic
GGAGGATGCTTTACGGGACC+TGG 0.481852 1.1:-12527784 MS.gene31700:CDS
GAATATAGGACTGTTTCGGT+TGG 0.481982 1.1:+12527644 None:intergenic
GTTAGCTCGTCATCATTGTC+CGG 0.486841 1.1:-12528335 MS.gene31700:CDS
ATGCTTTACGGGACCTGGAA+TGG 0.494601 1.1:-12527779 MS.gene31700:CDS
GACGTGAGCTCCAGTCGCAA+GGG 0.498267 1.1:+12527568 None:intergenic
AATCAAGTAATCATCATTGT+TGG 0.499207 1.1:+12527122 None:intergenic
TGTTTGGTCAGCACAAATGT+AGG 0.504596 1.1:+12527258 None:intergenic
ATCGTAAAGTGTTGGAGCTA+CGG 0.505579 1.1:-12527224 MS.gene31700:CDS
AAGGTTGGTGTCGGAAATGA+TGG 0.507635 1.1:+12528415 None:intergenic
ACAGTGATGGTAGGTAAGGC+GGG 0.516162 1.1:+12527486 None:intergenic
GGTGGTTTGGAAAATGTTGT+TGG 0.523159 1.1:+12528520 None:intergenic
TACCGACAATTTCATTGTTG+AGG 0.524862 1.1:-12528477 MS.gene31700:CDS
AAACGATTCCTCAACAACAT+TGG 0.525151 1.1:+12527752 None:intergenic
TACCGGAACACACAGGTCTC+CGG 0.525459 1.1:+12528253 None:intergenic
ATTGACCACGGTAACGGTGG+AGG 0.527850 1.1:+12528361 None:intergenic
GCCCGTAGCAAGAGGACTCG+TGG 0.533598 1.1:-12527594 MS.gene31700:CDS
AAGATACAAGTCGGGTCGAT+TGG 0.534399 1.1:-12527307 MS.gene31700:CDS
TTCCGACACCAACCTTGACT+CGG 0.534554 1.1:-12528408 MS.gene31700:CDS
AGGCATGAAGGTGCGAAACC+AGG 0.535516 1.1:-12527694 MS.gene31700:CDS
CCCACGAGTCCTCTTGCTAC+GGG 0.537511 1.1:+12527593 None:intergenic
GACGGAGGGTCAGGTGGAGA+TGG 0.539597 1.1:-12527420 MS.gene31700:CDS
GTTGTTGTCGGAGGAAAAGG+TGG 0.541025 1.1:+12528502 None:intergenic
GAGGGTCAGGTGGAGATGGT+CGG 0.544742 1.1:-12527416 MS.gene31700:CDS
CTGCTTCCTTGGAACAGTGA+TGG 0.548423 1.1:+12527473 None:intergenic
CAACACTTTACGATGCGAGT+TGG 0.550252 1.1:+12527233 None:intergenic
TGGAACAGTGATGGTAGGTA+AGG 0.550597 1.1:+12527482 None:intergenic
CATCACTGTTCCAAGGAAGC+AGG 0.554828 1.1:-12527472 MS.gene31700:CDS
AAAGACTGCTCCAAGAAGAA+AGG 0.555772 1.1:-12527448 MS.gene31700:CDS
AACGCTATTGACCACGGTAA+CGG 0.557909 1.1:+12528355 None:intergenic
GACAGTGACGGAGGGTCAGG+TGG 0.558691 1.1:-12527426 MS.gene31700:CDS
ACAAATGTAGGGCTAGCTGC+AGG 0.560737 1.1:+12527270 None:intergenic
AGACGTGAGCTCCAGTCGCA+AGG 0.560751 1.1:+12527567 None:intergenic
GTCCTCAACAATGAAATTGT+CGG 0.566137 1.1:+12528475 None:intergenic
AACAGTGATGGTAGGTAAGG+CGG 0.571647 1.1:+12527485 None:intergenic
TAAAATCAGGTCCCATATGT+TGG 0.573858 1.1:+12527097 None:intergenic
GTTTGGTCAGCACAAATGTA+GGG 0.574012 1.1:+12527259 None:intergenic
CCCGTAGCAAGAGGACTCGT+GGG 0.574146 1.1:-12527593 MS.gene31700:CDS
TTGGGTTGGGAAAGCTCACG+GGG 0.578980 1.1:+12527663 None:intergenic
CAGCTAAGGCCCGTAGCAAG+AGG 0.579929 1.1:-12527602 MS.gene31700:CDS
GGCCGAGTCAAGGTTGGTGT+CGG 0.584263 1.1:+12528406 None:intergenic
TTAGGCCCGGTTCGCCATTG+TGG 0.587065 1.1:+12527363 None:intergenic
TTAGCTCGTCATCATTGTCC+GGG 0.587117 1.1:-12528334 MS.gene31700:CDS
GCAACTGATATCAGGCATGA+AGG 0.589173 1.1:-12527706 MS.gene31700:CDS
TTGTGGTGTAAGATACAAGT+CGG 0.589234 1.1:-12527316 MS.gene31700:CDS
AGTTGACTGTGGCCGAGTCA+AGG 0.589310 1.1:+12528396 None:intergenic
ATTCCCTACGTAGGAATGGA+AGG 0.590186 1.1:-12528548 None:intergenic
ATTGTCGGTAGTGTTGTTGT+CGG 0.590207 1.1:+12528490 None:intergenic
GGTCAGGTGGAGATGGTCGG+AGG 0.590443 1.1:-12527413 MS.gene31700:CDS
GTCGGTAGTGTTGTTGTCGG+AGG 0.590547 1.1:+12528493 None:intergenic
TACCTACCATCACTGTTCCA+AGG 0.594004 1.1:-12527479 MS.gene31700:CDS
CGTCGTCGACGTCATTGTTG+AGG 0.596242 1.1:+12528444 None:intergenic
ATGGCTTTCCAATGTTGTTG+AGG 0.597478 1.1:-12527760 MS.gene31700:CDS
AACGACATTAGGGTCGCACC+CGG 0.603894 1.1:+12528316 None:intergenic
CAACTCGCATCGTAAAGTGT+TGG 0.604228 1.1:-12527232 MS.gene31700:CDS
CTCTTCCTCCACCGTTACCG+TGG 0.606184 1.1:-12528366 MS.gene31700:CDS
TGTGGTGTAAGATACAAGTC+GGG 0.608929 1.1:-12527315 MS.gene31700:CDS
GTTGGAGCTACGGAGGCAGA+AGG 0.611025 1.1:-12527214 MS.gene31700:CDS
CGTCATTGTTGAGGTTAAGG+AGG 0.612987 1.1:+12528453 None:intergenic
GACTGTGGCCGAGTCAAGGT+TGG 0.614521 1.1:+12528400 None:intergenic
ACGTGAGCTCCAGTCGCAAG+GGG 0.619626 1.1:+12527569 None:intergenic
GCTATTGACCACGGTAACGG+TGG 0.631846 1.1:+12528358 None:intergenic
GAAGAAAGGACAGTGACGGA+GGG 0.637953 1.1:-12527434 MS.gene31700:CDS
AAAACATTATGCAACGCTTG+TGG 0.638941 1.1:-12527333 MS.gene31700:CDS
CCAAGAAGAAAGGACAGTGA+CGG 0.640630 1.1:-12527438 MS.gene31700:CDS
AGAAGAAAGGACAGTGACGG+AGG 0.643844 1.1:-12527435 MS.gene31700:CDS
TTCCTTGGAACAGTGATGGT+AGG 0.661750 1.1:+12527477 None:intergenic
AGTGATGGTAGGTAAGGCGG+GGG 0.663791 1.1:+12527488 None:intergenic
GTAAAGTGTTGGAGCTACGG+AGG 0.667005 1.1:-12527221 MS.gene31700:CDS
CAGTGATGGTAGGTAAGGCG+GGG 0.673174 1.1:+12527487 None:intergenic
GGAGGCAGAAGGAAATGTTG+CGG 0.675658 1.1:-12527203 MS.gene31700:CDS
AGTTCTGACTCAGAAGAAGA+CGG 0.679246 1.1:+12527522 None:intergenic
GGTAACGGTGGAGGAAGAGG+CGG 0.684503 1.1:+12528370 None:intergenic
TATAATATACCGGAACACAC+AGG 0.691414 1.1:+12528246 None:intergenic
AAACAGTCCTATATTCAACA+AGG 0.707758 1.1:-12527637 MS.gene31700:CDS
CGAGCTAACGCTATTGACCA+CGG 0.719172 1.1:+12528349 None:intergenic
CGTGAGCTCCAGTCGCAAGG+GGG 0.723454 1.1:+12527570 None:intergenic
TGACAGACAAGACACCACAA+TGG 0.724676 1.1:-12527377 MS.gene31700:CDS
AGAGGCGGTTGAGTTGACTG+TGG 0.744548 1.1:+12528385 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CATATACACATATAATATAC+CGG + chr1.1:12527383-12527402 None:intergenic 20.0%
!!! TTTGTGTTGAAATTTGAAAT+AGG - chr1.1:12527811-12527830 MS.gene31700:intron 20.0%
!!! TTTTGTGATACTCAATAATT+TGG + chr1.1:12527682-12527701 None:intergenic 20.0%
! AAAACTTCCTTGTTGAATAT+AGG + chr1.1:12527989-12528008 None:intergenic 25.0%
! AATCAAGTAATCATCATTGT+TGG + chr1.1:12528497-12528516 None:intergenic 25.0%
! CTGAAATTATTGGCAAAAAA+GGG + chr1.1:12527564-12527583 None:intergenic 25.0%
!! TATATTCAACAAGGAAGTTT+TGG - chr1.1:12527988-12528007 MS.gene31700:intron 25.0%
!!! GTTTTTTCTCCTGAAATTAT+TGG + chr1.1:12527574-12527593 None:intergenic 25.0%
AAACAGTCCTATATTCAACA+AGG - chr1.1:12527979-12527998 MS.gene31700:intron 30.0%
CCTGAAATTATTGGCAAAAA+AGG + chr1.1:12527565-12527584 None:intergenic 30.0%
TACTCTGATGTTGTAAAATC+TGG + chr1.1:12528459-12528478 None:intergenic 30.0%
TACTTGATTCACCAACATAT+GGG - chr1.1:12528508-12528527 MS.gene31700:CDS 30.0%
TAGGCTAAATACAACAGAAT+GGG - chr1.1:12527601-12527620 MS.gene31700:CDS 30.0%
TGTTGAATATAGGACTGTTT+CGG + chr1.1:12527979-12527998 None:intergenic 30.0%
TTACTTGATTCACCAACATA+TGG - chr1.1:12528507-12528526 MS.gene31700:CDS 30.0%
! GCATGAGTTAGAAATTTTCA+TGG + chr1.1:12527499-12527518 None:intergenic 30.0%
! GTGTTGAAATTTGAAATAGG+AGG - chr1.1:12527814-12527833 MS.gene31700:intron 30.0%
! TGTTTCAAGAGCATATATAG+AGG - chr1.1:12527777-12527796 MS.gene31700:CDS 30.0%
!!! CCTTTTTTGCCAATAATTTC+AGG - chr1.1:12527562-12527581 MS.gene31700:CDS 30.0%
!!! GTTGTAAAATCTGGTTTTGT+TGG + chr1.1:12528450-12528469 None:intergenic 30.0%
AAAACATTATGCAACGCTTG+TGG - chr1.1:12528283-12528302 MS.gene31700:CDS 35.0%
AAACGATTCCTCAACAACAT+TGG + chr1.1:12527867-12527886 None:intergenic 35.0%
AAATTCTGACAGCCAATATC+AGG + chr1.1:12527326-12527345 None:intergenic 35.0%
AATATAGGACTGTTTCGGTT+GGG + chr1.1:12527974-12527993 None:intergenic 35.0%
AGGAGAAAAAACAACTAGCT+AGG - chr1.1:12527582-12527601 MS.gene31700:CDS 35.0%
AGGCTAAATACAACAGAATG+GGG - chr1.1:12527602-12527621 MS.gene31700:CDS 35.0%
CAAAAGATGCAACTGATATC+AGG - chr1.1:12527902-12527921 MS.gene31700:intron 35.0%
CAATATCAGGAACGACATTA+GGG + chr1.1:12527313-12527332 None:intergenic 35.0%
CTAGGCTAAATACAACAGAA+TGG - chr1.1:12527600-12527619 MS.gene31700:CDS 35.0%
GTCCTCAACAATGAAATTGT+CGG + chr1.1:12527144-12527163 None:intergenic 35.0%
TAAAATCAGGTCCCATATGT+TGG + chr1.1:12528522-12528541 None:intergenic 35.0%
TACATCATCTGCCTAAAATC+AGG + chr1.1:12528535-12528554 None:intergenic 35.0%
TACCGACAATTTCATTGTTG+AGG - chr1.1:12527139-12527158 MS.gene31700:CDS 35.0%
TATAATATACCGGAACACAC+AGG + chr1.1:12527373-12527392 None:intergenic 35.0%
TGAAATAGGAGGATGCTTTA+CGG - chr1.1:12527825-12527844 MS.gene31700:intron 35.0%
TTGTGGTGTAAGATACAAGT+CGG - chr1.1:12528300-12528319 MS.gene31700:CDS 35.0%
! AACATATGGGACCTGATTTT+AGG - chr1.1:12528521-12528540 MS.gene31700:CDS 35.0%
!! AAGCGTTGCATAATGTTTTC+GGG + chr1.1:12528282-12528301 None:intergenic 35.0%
!! CAAGCGTTGCATAATGTTTT+CGG + chr1.1:12528283-12528302 None:intergenic 35.0%
!!! AAATCTGGTTTTGTTGGTGT+TGG + chr1.1:12528444-12528463 None:intergenic 35.0%
AAAGACTGCTCCAAGAAGAA+AGG - chr1.1:12528168-12528187 MS.gene31700:intron 40.0%
ATGGCTTTCCAATGTTGTTG+AGG - chr1.1:12527856-12527875 MS.gene31700:intron 40.0%
CCAATATCAGGAACGACATT+AGG + chr1.1:12527314-12527333 None:intergenic 40.0%
CCTAATGTCGTTCCTGATAT+TGG - chr1.1:12527311-12527330 MS.gene31700:CDS 40.0%
GAAATAGGAGGATGCTTTAC+GGG - chr1.1:12527826-12527845 MS.gene31700:intron 40.0%
GAATATAGGACTGTTTCGGT+TGG + chr1.1:12527975-12527994 None:intergenic 40.0%
GTTTGGTCAGCACAAATGTA+GGG + chr1.1:12528360-12528379 None:intergenic 40.0%
TGTGGTGTAAGATACAAGTC+GGG - chr1.1:12528301-12528320 MS.gene31700:CDS 40.0%
TGTTTGGTCAGCACAAATGT+AGG + chr1.1:12528361-12528380 None:intergenic 40.0%
! AGTTCTGACTCAGAAGAAGA+CGG + chr1.1:12528097-12528116 None:intergenic 40.0%
! ATTGTCGGTAGTGTTGTTGT+CGG + chr1.1:12527129-12527148 None:intergenic 40.0%
! GGTGGTTTGGAAAATGTTGT+TGG + chr1.1:12527099-12527118 None:intergenic 40.0%
! TTTTCTGACAGCCATTTCTC+CGG - chr1.1:12527344-12527363 MS.gene31700:CDS 40.0%
!! ATCGTAAAGTGTTGGAGCTA+CGG - chr1.1:12528392-12528411 MS.gene31700:CDS 40.0%
!! CATAATGTTTTCGGGCCATT+AGG + chr1.1:12528274-12528293 None:intergenic 40.0%
AACAGTGATGGTAGGTAAGG+CGG + chr1.1:12528134-12528153 None:intergenic 45.0%
AACGCTATTGACCACGGTAA+CGG + chr1.1:12527264-12527283 None:intergenic 45.0%
AAGATACAAGTCGGGTCGAT+TGG - chr1.1:12528309-12528328 MS.gene31700:CDS 45.0%
ACAGAATGGGGCACATTTCA+CGG - chr1.1:12527614-12527633 MS.gene31700:CDS 45.0%
CAACACTTTACGATGCGAGT+TGG + chr1.1:12528386-12528405 None:intergenic 45.0%
CAACATTGGAAAGCCATTCC+AGG + chr1.1:12527853-12527872 None:intergenic 45.0%
CAACTCGCATCGTAAAGTGT+TGG - chr1.1:12528384-12528403 MS.gene31700:CDS 45.0%
CCAAGAAGAAAGGACAGTGA+CGG - chr1.1:12528178-12528197 MS.gene31700:intron 45.0%
CGACGTCATTGTTGAGGTTA+AGG + chr1.1:12527169-12527188 None:intergenic 45.0%
CGTCATTGTTGAGGTTAAGG+AGG + chr1.1:12527166-12527185 None:intergenic 45.0%
GCAACTGATATCAGGCATGA+AGG - chr1.1:12527910-12527929 MS.gene31700:intron 45.0%
GTTAGCTCGTCATCATTGTC+CGG - chr1.1:12527281-12527300 MS.gene31700:CDS 45.0%
TACCTACCATCACTGTTCCA+AGG - chr1.1:12528137-12528156 MS.gene31700:intron 45.0%
TGACAGACAAGACACCACAA+TGG - chr1.1:12528239-12528258 MS.gene31700:intron 45.0%
TGGAACAGTGATGGTAGGTA+AGG + chr1.1:12528137-12528156 None:intergenic 45.0%
TTAGCTCGTCATCATTGTCC+GGG - chr1.1:12527282-12527301 MS.gene31700:CDS 45.0%
TTCCTTGGAACAGTGATGGT+AGG + chr1.1:12528142-12528161 None:intergenic 45.0%
! AGTGTTGTTGTCGGAGGAAA+AGG + chr1.1:12527120-12527139 None:intergenic 45.0%
!! AAGGTTGGTGTCGGAAATGA+TGG + chr1.1:12527204-12527223 None:intergenic 45.0%
!! TGAGGAATCGTTTTCAAGCG+AGG - chr1.1:12527874-12527893 MS.gene31700:intron 45.0%
!!! GGAAGTTTTGGTTCCAGCTA+AGG - chr1.1:12528000-12528019 MS.gene31700:intron 45.0%
AAGACACCACAATGGCGAAC+CGG - chr1.1:12528247-12528266 MS.gene31700:intron 50.0%
ACAGTGATGGTAGGTAAGGC+GGG + chr1.1:12528133-12528152 None:intergenic 50.0%
AGAAGAAAGGACAGTGACGG+AGG - chr1.1:12528181-12528200 MS.gene31700:intron 50.0%
AGGACTGTTTCGGTTGGGTT+GGG + chr1.1:12527969-12527988 None:intergenic 50.0%
ATGCTTTACGGGACCTGGAA+TGG - chr1.1:12527837-12527856 MS.gene31700:intron 50.0%
CATCACTGTTCCAAGGAAGC+AGG - chr1.1:12528144-12528163 MS.gene31700:intron 50.0%
CCGTCACTGTCCTTTCTTCT+TGG + chr1.1:12528181-12528200 None:intergenic 50.0%
CGAGCTAACGCTATTGACCA+CGG + chr1.1:12527270-12527289 None:intergenic 50.0%
CTGCTTCCTTGGAACAGTGA+TGG + chr1.1:12528146-12528165 None:intergenic 50.0%
GAGGCAGAAGGAAATGTTGC+GGG - chr1.1:12528414-12528433 MS.gene31700:CDS 50.0%
GGAGGCAGAAGGAAATGTTG+CGG - chr1.1:12528413-12528432 MS.gene31700:CDS 50.0%
GTTGTTGTCGGAGGAAAAGG+TGG + chr1.1:12527117-12527136 None:intergenic 50.0%
TAGGACTGTTTCGGTTGGGT+TGG + chr1.1:12527970-12527989 None:intergenic 50.0%
TGTCGGAGGAAAAGGTGGTT+TGG + chr1.1:12527112-12527131 None:intergenic 50.0%
TTCCGACACCAACCTTGACT+CGG - chr1.1:12527208-12527227 MS.gene31700:CDS 50.0%
! ACGATGCGAGTTGGAGTGTT+TGG + chr1.1:12528377-12528396 None:intergenic 50.0%
! GAAGAAAGGACAGTGACGGA+GGG - chr1.1:12528182-12528201 MS.gene31700:intron 50.0%
! GTCGATTGGTGCCTGAATAC+AGG - chr1.1:12528323-12528342 MS.gene31700:CDS 50.0%
!! ACAAATGTAGGGCTAGCTGC+AGG + chr1.1:12528349-12528368 None:intergenic 50.0%
!! GTAAAGTGTTGGAGCTACGG+AGG - chr1.1:12528395-12528414 MS.gene31700:CDS 50.0%
AACGACATTAGGGTCGCACC+CGG + chr1.1:12527303-12527322 None:intergenic 55.0%
AGACACCACAATGGCGAACC+GGG - chr1.1:12528248-12528267 MS.gene31700:intron 55.0%
AGTGATGGTAGGTAAGGCGG+GGG + chr1.1:12528131-12528150 None:intergenic 55.0%
AGTTGACTGTGGCCGAGTCA+AGG + chr1.1:12527223-12527242 None:intergenic 55.0%
CACACAGGTCTCCGGAGAAA+TGG + chr1.1:12527358-12527377 None:intergenic 55.0%
CAGTGATGGTAGGTAAGGCG+GGG + chr1.1:12528132-12528151 None:intergenic 55.0%
CGTCGTCGACGTCATTGTTG+AGG + chr1.1:12527175-12527194 None:intergenic 55.0%
CTAGCTGCAGGCCTGTATTC+AGG + chr1.1:12528337-12528356 None:intergenic 55.0%
GCAGTCTTTGCCTGCTTCCT+TGG + chr1.1:12528157-12528176 None:intergenic 55.0%
TACCGGAACACACAGGTCTC+CGG + chr1.1:12527366-12527385 None:intergenic 55.0%
! AGGCATGAAGGTGCGAAACC+AGG - chr1.1:12527922-12527941 MS.gene31700:intron 55.0%
! ATTGACCACGGTAACGGTGG+AGG + chr1.1:12527258-12527277 None:intergenic 55.0%
! GCTATTGACCACGGTAACGG+TGG + chr1.1:12527261-12527280 None:intergenic 55.0%
! GTCGGTAGTGTTGTTGTCGG+AGG + chr1.1:12527126-12527145 None:intergenic 55.0%
!! AGAGGCGGTTGAGTTGACTG+TGG + chr1.1:12527234-12527253 None:intergenic 55.0%
!! GGTTGGGTTGGGAAAGCTCA+CGG + chr1.1:12527958-12527977 None:intergenic 55.0%
!! GTTGGGTTGGGAAAGCTCAC+GGG + chr1.1:12527957-12527976 None:intergenic 55.0%
!! TGTTTTCGGGCCATTAGGCC+CGG + chr1.1:12528269-12528288 None:intergenic 55.0%
!! TTGGGTTGGGAAAGCTCACG+GGG + chr1.1:12527956-12527975 None:intergenic 55.0%
ACGTGAGCTCCAGTCGCAAG+GGG + chr1.1:12528050-12528069 None:intergenic 60.0%
AGACGTGAGCTCCAGTCGCA+AGG + chr1.1:12528052-12528071 None:intergenic 60.0%
ATGGTAGGTAAGGCGGGGGT+AGG + chr1.1:12528127-12528146 None:intergenic 60.0%
CAATGGCGAACCGGGCCTAA+TGG - chr1.1:12528256-12528275 MS.gene31700:CDS 60.0%
CAGCTAAGGCCCGTAGCAAG+AGG - chr1.1:12528014-12528033 MS.gene31700:intron 60.0%
CCCACGAGTCCTCTTGCTAC+GGG + chr1.1:12528026-12528045 None:intergenic 60.0%
CCCGTAGCAAGAGGACTCGT+GGG - chr1.1:12528023-12528042 MS.gene31700:intron 60.0%
CTCCGGAGACCTGTGTGTTC+CGG - chr1.1:12527361-12527380 MS.gene31700:CDS 60.0%
CTCTTCCTCCACCGTTACCG+TGG - chr1.1:12527250-12527269 MS.gene31700:CDS 60.0%
CTCTTGCTACGGGCCTTAGC+TGG + chr1.1:12528016-12528035 None:intergenic 60.0%
GACGTGAGCTCCAGTCGCAA+GGG + chr1.1:12528051-12528070 None:intergenic 60.0%
GACTGTGGCCGAGTCAAGGT+TGG + chr1.1:12527219-12527238 None:intergenic 60.0%
GAGGGTCAGGTGGAGATGGT+CGG - chr1.1:12528200-12528219 MS.gene31700:intron 60.0%
GCCCACGAGTCCTCTTGCTA+CGG + chr1.1:12528027-12528046 None:intergenic 60.0%
GGAGGATGCTTTACGGGACC+TGG - chr1.1:12527832-12527851 MS.gene31700:intron 60.0%
GTTGGAGCTACGGAGGCAGA+AGG - chr1.1:12528402-12528421 MS.gene31700:CDS 60.0%
! AAGGACAGTGACGGAGGGTC+AGG - chr1.1:12528187-12528206 MS.gene31700:intron 60.0%
! CACGGTAACGGTGGAGGAAG+AGG + chr1.1:12527252-12527271 None:intergenic 60.0%
! GGTAACGGTGGAGGAAGAGG+CGG + chr1.1:12527249-12527268 None:intergenic 60.0%
! TTAGGCCCGGTTCGCCATTG+TGG + chr1.1:12528256-12528275 None:intergenic 60.0%
!! GGCCGAGTCAAGGTTGGTGT+CGG + chr1.1:12527213-12527232 None:intergenic 60.0%
CGTGAGCTCCAGTCGCAAGG+GGG + chr1.1:12528049-12528068 None:intergenic 65.0%
GACGGAGGGTCAGGTGGAGA+TGG - chr1.1:12528196-12528215 MS.gene31700:intron 65.0%
GCCCGTAGCAAGAGGACTCG+TGG - chr1.1:12528022-12528041 MS.gene31700:intron 65.0%
GCTCACGGGGTTCTTCGTCC+TGG + chr1.1:12527943-12527962 None:intergenic 65.0%
GGTCAGGTGGAGATGGTCGG+AGG - chr1.1:12528203-12528222 MS.gene31700:intron 65.0%
! GACAGTGACGGAGGGTCAGG+TGG - chr1.1:12528190-12528209 MS.gene31700:intron 65.0%
CTCGTGGGCCCCCTTGCGAC+TGG - chr1.1:12528038-12528057 MS.gene31700:intron 75.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 12527083 12528555 12527083 ID=MS.gene31700
chr1.1 mRNA 12527083 12528555 12527083 ID=MS.gene31700.t1;Parent=MS.gene31700
chr1.1 exon 12528256 12528555 12528256 ID=MS.gene31700.t1.exon1;Parent=MS.gene31700.t1
chr1.1 CDS 12528256 12528555 12528256 ID=cds.MS.gene31700.t1;Parent=MS.gene31700.t1
chr1.1 exon 12527083 12527805 12527083 ID=MS.gene31700.t1.exon2;Parent=MS.gene31700.t1
chr1.1 CDS 12527083 12527805 12527083 ID=cds.MS.gene31700.t1;Parent=MS.gene31700.t1
Gene Sequence

>MS.gene31700

ATGGAAGGCACGACCAACAACATTTTCCAAACCACCTTTTCCTCCGACAACAACACTACCGACAATTTCATTGTTGAGGACCTCCTTAACCTCAACAATGACGTCGACGACGACGCTACCATCATTTCCGACACCAACCTTGACTCGGCCACAGTCAACTCAACCGCCTCTTCCTCCACCGTTACCGTGGTCAATAGCGTTAGCTCGTCATCATTGTCCGGGTGCGACCCTAATGTCGTTCCTGATATTGGCTGTCAGAATTTTTCTGACAGCCATTTCTCCGGAGACCTGTGTGTTCCGGTATATTATATGTGTATATGCATTATCAATTATTATCTTTTTAAAAAAATATAAAAAGCACATAGACACTTCACAATTTTCTAACTCGTTTAATTTGACTTTTTCTATTAGTTCCATGAAAATTTCTAACTCATGCACGTATAACATATAACATCACATTGCATTTGAAGATTTTGAACCCTTTTTTGCCAATAATTTCAGGAGAAAAAACAACTAGCTAGGCTAAATACAACAGAATGGGGCACATTTCACGGTTCGAAAACTAAATTTTATTGCAACATTTGTTTCAATTATTACCAAATTATTGAGTATCACAAAAATAGTACACGTGAAAGCATTAGTATGACTATGAACAAAAAATGTGTATGATTGATTACTTGTGTACAATGTAATATGTTTCAAGAGCATATATAGAGGAATAATTATTGTTTGTGTTGAAATTTGAAATAGGAGGATGCTTTACGGGACCTGGAATGGCTTTCCAATGTTGTTGAGGAATCGTTTTCAAGCGAGGATCTTCAAAAGATGCAACTGATATCAGGCATGAAGGTGCGAAACCAGGACGAAGAACCCCGTGAGCTTTCCCAACCCAACCGAAACAGTCCTATATTCAACAAGGAAGTTTTGGTTCCAGCTAAGGCCCGTAGCAAGAGGACTCGTGGGCCCCCTTGCGACTGGAGCTCACGTCTTCTTGTTTTGTCTCAAACAACACCGTCTTCTTCTGAGTCAGAACTTCTTATTCCTACCCCCGCCTTACCTACCATCACTGTTCCAAGGAAGCAGGCAAAGACTGCTCCAAGAAGAAAGGACAGTGACGGAGGGTCAGGTGGAGATGGTCGGAGGTGTCTGCATTGCATGACAGACAAGACACCACAATGGCGAACCGGGCCTAATGGCCCGAAAACATTATGCAACGCTTGTGGTGTAAGATACAAGTCGGGTCGATTGGTGCCTGAATACAGGCCTGCAGCTAGCCCTACATTTGTGCTGACCAAACACTCCAACTCGCATCGTAAAGTGTTGGAGCTACGGAGGCAGAAGGAAATGTTGCGGGCACACCAACACCAACAAAACCAGATTTTACAACATCAGAGTATGATGTTTGATATGTCCAACAATGATGATTACTTGATTCACCAACATATGGGACCTGATTTTAGGCAGATGATGTAG
Protein sequence

>MS.gene31700.t1

MEGTTNNIFQTTFSSDNNTTDNFIVEDLLNLNNDVDDDATIISDTNLDSATVNSTASSSTVTVVNSVSSSSLSGCDPNVVPDIGCQNFSDSHFSGDLCVPEDALRDLEWLSNVVEESFSSEDLQKMQLISGMKVRNQDEEPRELSQPNRNSPIFNKEVLVPAKARSKRTRGPPCDWSSRLLVLSQTTPSSSESELLIPTPALPTITVPRKQAKTAPRRKDSDGGSGGDGRRCLHCMTDKTPQWRTGPNGPKTLCNACGVRYKSGRLVPEYRPAASPTFVLTKHSNSHRKVLELRRQKEMLRAHQHQQNQILQHQSMMFDMSNNDDYLIHQHMGPDFRQMM