AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene31841

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31841.t1 MTR_1g029100 83.146 89 14 1 42 130 1 88 1.27e-46 147
MS.gene31841.t1 MTR_1g029070 41.844 141 72 3 1 132 1 140 1.70e-27 100
MS.gene31841.t1 MTR_1g072790 38.393 112 59 1 38 139 23 134 1.95e-21 85.5
MS.gene31841.t1 MTR_4g133952 38.889 90 55 0 44 133 58 147 6.67e-21 85.5
MS.gene31841.t1 MTR_3g058980 38.462 104 63 1 44 147 27 129 6.82e-21 84.3
MS.gene31841.t1 MTR_1g088860 35.200 125 79 1 9 133 3 125 6.90e-21 84.3
MS.gene31841.t1 MTR_7g061270 36.607 112 68 1 44 152 26 137 1.26e-20 83.2
MS.gene31841.t1 MTR_7g100650 36.607 112 68 1 44 152 26 137 1.48e-20 83.2
MS.gene31841.t1 MTR_1g039040 40.816 98 57 1 44 141 6 102 2.58e-20 82.8
MS.gene31841.t1 MTR_8g091720 38.679 106 64 1 44 149 28 132 3.12e-20 82.8
MS.gene31841.t1 MTR_5g095740 41.111 90 53 0 44 133 23 112 6.38e-20 81.6
MS.gene31841.t1 MTR_2g026710 40.426 94 55 1 44 137 17 109 8.38e-20 80.9
MS.gene31841.t1 MTR_4g119500 32.520 123 83 0 26 148 4 126 2.25e-19 79.0
MS.gene31841.t1 MTR_0392s0020 33.628 113 64 1 21 133 6 107 2.45e-19 79.0
MS.gene31841.t1 MTR_4g133938 40.230 87 52 0 47 133 22 108 2.95e-19 79.0
MS.gene31841.t1 MTR_8g093920 40.449 89 53 0 44 132 40 128 6.12e-18 77.4
MS.gene31841.t1 MTR_1g083070 35.294 85 55 0 49 133 9 93 2.90e-17 73.6
MS.gene31841.t1 MTR_1g028480 38.372 86 39 1 80 151 25 110 1.33e-14 66.2
MS.gene31841.t1 MTR_5g095900 34.524 84 55 0 49 132 21 104 1.46e-13 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31841.t1 AT2G38880 40.566 106 62 1 44 148 21 126 1.40e-22 87.4
MS.gene31841.t1 AT3G53340 40.541 111 63 1 44 151 29 139 2.20e-22 87.8
MS.gene31841.t1 AT3G53340 40.541 111 63 1 44 151 29 139 2.20e-22 87.8
MS.gene31841.t1 AT2G38880 39.815 108 62 1 44 148 21 128 3.05e-22 86.7
MS.gene31841.t1 AT2G38880 39.815 108 62 1 44 148 21 128 3.13e-22 86.7
MS.gene31841.t1 AT2G38880 39.815 108 62 1 44 148 21 128 3.13e-22 86.7
MS.gene31841.t1 AT2G38880 39.815 108 62 1 44 148 21 128 3.13e-22 86.7
MS.gene31841.t1 AT2G37060 39.640 111 64 1 44 151 30 140 8.08e-22 86.3
MS.gene31841.t1 AT2G37060 39.640 111 64 1 44 151 30 140 8.08e-22 86.3
MS.gene31841.t1 AT2G37060 39.640 111 64 1 44 151 30 140 8.08e-22 86.3
MS.gene31841.t1 AT2G38880 41.111 90 53 0 44 133 21 110 1.64e-20 81.3
MS.gene31841.t1 AT4G14540 41.111 90 53 0 44 133 21 110 1.90e-20 82.4
MS.gene31841.t1 AT2G38880 41.111 90 53 0 44 133 21 110 3.72e-20 82.0
MS.gene31841.t1 AT2G38880 41.111 90 53 0 44 133 21 110 3.72e-20 82.0
MS.gene31841.t1 AT5G47640 42.222 90 52 0 44 133 27 116 4.56e-20 82.4
MS.gene31841.t1 AT1G21970 32.886 149 83 2 1 133 1 148 6.90e-20 82.8
MS.gene31841.t1 AT2G38880 41.111 90 53 0 44 133 21 110 2.06e-19 79.7
MS.gene31841.t1 AT2G38880 41.111 90 53 0 44 133 21 110 2.06e-19 79.7
MS.gene31841.t1 AT2G38880 41.111 90 53 0 44 133 21 110 2.06e-19 79.7
MS.gene31841.t1 AT2G47810 37.374 99 62 0 34 132 41 139 2.25e-19 79.7
MS.gene31841.t1 AT3G53340 42.222 90 52 0 44 133 29 118 2.71e-19 78.6
MS.gene31841.t1 AT5G47670 36.000 100 64 0 44 143 29 128 4.79e-19 80.1
MS.gene31841.t1 AT5G47670 36.000 100 64 0 44 143 58 157 7.67e-19 80.1
MS.gene31841.t1 AT5G47670 36.000 100 64 0 44 143 58 157 7.67e-19 80.1
MS.gene31841.t1 AT3G53340 42.222 90 52 0 44 133 29 118 5.15e-18 75.9
MS.gene31841.t1 AT3G53340 42.222 90 52 0 44 133 29 118 5.15e-18 75.9
MS.gene31841.t1 AT2G13570 40.449 89 53 0 44 132 36 124 5.80e-17 74.7
MS.gene31841.t1 AT1G09030 31.522 92 63 0 42 133 1 92 7.41e-16 70.1

Find 39 sgRNAs with CRISPR-Local

Find 82 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCACTTCCTCGCCCATTGC+TGG 0.310496 1.1:-14625449 None:intergenic
TTTCCGCTGATGATTTACTA+TGG 0.336830 1.1:+14625639 MS.gene31841:CDS
ATTTACTATGGACGATGAAT+AGG 0.363355 1.1:+14625651 MS.gene31841:CDS
GAGGAACATGTTCGGTCTCT+TGG 0.363705 1.1:+14625683 MS.gene31841:CDS
GATGCTCTACATTGGCTTGT+TGG 0.407680 1.1:-14625607 None:intergenic
GATGAATAGGTTGGGCTTTG+AGG 0.431149 1.1:+14625664 MS.gene31841:CDS
AGGAACATGTTCGGTCTCTT+GGG 0.445239 1.1:+14625684 MS.gene31841:CDS
CTATGGACGATGAATAGGTT+GGG 0.452990 1.1:+14625656 MS.gene31841:CDS
ACCAACAACAACACTGGCAT+TGG 0.458777 1.1:+14625404 MS.gene31841:CDS
TCCAATGCCAGTGTTGTTGT+TGG 0.470529 1.1:-14625405 None:intergenic
TCAACGTTGGTCATCATCAT+TGG 0.472149 1.1:-14625768 None:intergenic
CGCAAGAACATGGATGGGTC+AGG 0.479316 1.1:+14625725 MS.gene31841:CDS
GACTCTTGTCACGTGACTGA+TGG 0.479441 1.1:-14625480 None:intergenic
AACTATCGCAAGAACATGGA+TGG 0.501574 1.1:+14625719 MS.gene31841:CDS
TGTGTCTAAATTTAAGTGCA+AGG 0.501776 1.1:+14625568 MS.gene31841:CDS
TGGGCTTTGAGGAACATGTT+CGG 0.504221 1.1:+14625675 MS.gene31841:CDS
CAACATTCAACTCCAGCAAT+GGG 0.504417 1.1:+14625437 MS.gene31841:CDS
CCATTGCTGGAGTTGAATGT+TGG 0.510977 1.1:-14625436 None:intergenic
GCCAGTGTTGTTGTTGGTGG+CGG 0.514598 1.1:-14625399 None:intergenic
AATGCCAGTGTTGTTGTTGG+TGG 0.520685 1.1:-14625402 None:intergenic
CGTTCGACACAATCAAGATC+TGG 0.523771 1.1:+14624304 MS.gene31841:CDS
TCATCAGTGATTATTGTGTC+TGG 0.528332 1.1:-14625521 None:intergenic
ACTATGGACGATGAATAGGT+TGG 0.532041 1.1:+14625655 MS.gene31841:CDS
CCAACATTCAACTCCAGCAA+TGG 0.549683 1.1:+14625436 MS.gene31841:CDS
ATTCTCTTGATGCTCTACAT+TGG 0.555958 1.1:-14625615 None:intergenic
CGAACGGTTGTTGTTAGATG+AGG 0.556426 1.1:-14624287 None:intergenic
AATAATCACTGATGATGCTA+AGG 0.558102 1.1:+14625529 MS.gene31841:CDS
TTCAACTCCAGCAATGGGCG+AGG 0.564498 1.1:+14625442 MS.gene31841:CDS
ACTCTTGTCACGTGACTGAT+GGG 0.569137 1.1:-14625479 None:intergenic
ATGGATGGGTCAGGTGCTAG+TGG 0.576300 1.1:+14625734 MS.gene31841:CDS
CGTTAATTTCATATCAACGT+TGG 0.576753 1.1:-14625781 None:intergenic
GAACGGTTGTTGTTAGATGA+GGG 0.584819 1.1:-14624286 None:intergenic
CAGATCTTGATTGTGTCGAA+CGG 0.594407 1.1:-14624303 None:intergenic
ACCGCCACCAACAACAACAC+TGG 0.598342 1.1:+14625398 MS.gene31841:CDS
TCCTGAAGTAATATGGAGCG+TGG 0.600798 1.1:+14624259 None:intergenic
TCAGTGATTATTGTGTCTGG+TGG 0.634070 1.1:-14625518 None:intergenic
ACTATCGCAAGAACATGGAT+GGG 0.635414 1.1:+14625720 MS.gene31841:CDS
TAAGTGCAAGGTCACAAGTG+AGG 0.636231 1.1:+14625580 MS.gene31841:CDS
CGTCCATAGTAAATCATCAG+CGG 0.656317 1.1:-14625642 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATAAATTAAAAATATTAA+AGG - chr1.1:14624709-14624728 None:intergenic 0.0%
!! ACTTTGTAAAATATTGAATA+TGG + chr1.1:14624404-14624423 MS.gene31841:intron 15.0%
!! AGTAAAAAAATTAAAATACG+AGG - chr1.1:14624387-14624406 None:intergenic 15.0%
!!! ATCTTGAAATTAATTTTGTT+CGG + chr1.1:14624796-14624815 MS.gene31841:intron 15.0%
!! AAATAAAGTAAAAGAGATAG+AGG - chr1.1:14624678-14624697 None:intergenic 20.0%
!! AATCTAATAGAAGCAATTAA+GGG - chr1.1:14624503-14624522 None:intergenic 20.0%
!!! TACCAAATCTTTTAATTTTG+AGG + chr1.1:14625314-14625333 MS.gene31841:intron 20.0%
!!! TGAATGAAATTTTGATTGAA+GGG + chr1.1:14624621-14624640 MS.gene31841:intron 20.0%
!!! TTGAATGAAATTTTGATTGA+AGG + chr1.1:14624620-14624639 MS.gene31841:intron 20.0%
!!! TTGTTAACCATTTTTGATAT+GGG + chr1.1:14624583-14624602 MS.gene31841:intron 20.0%
! AAAACAACTTTGCTAAGATT+TGG + chr1.1:14624846-14624865 MS.gene31841:intron 25.0%
! AACAAAACTGACGTATTTAA+TGG - chr1.1:14625017-14625036 None:intergenic 25.0%
! AAGAAATAAAGTGAGAGAAA+AGG + chr1.1:14624968-14624987 MS.gene31841:intron 25.0%
! ACAAAACTGACGTATTTAAT+GGG - chr1.1:14625016-14625035 None:intergenic 25.0%
! ATCTAATAGAAGCAATTAAG+GGG - chr1.1:14624502-14624521 None:intergenic 25.0%
! CTCCTCAAAATTAAAAGATT+TGG - chr1.1:14625319-14625338 None:intergenic 25.0%
! GAATCTAATAGAAGCAATTA+AGG - chr1.1:14624504-14624523 None:intergenic 25.0%
!! CTTTTCTTCTTTTTAATCGA+AGG - chr1.1:14625214-14625233 None:intergenic 25.0%
!! GTATTTTCCCATATCAAAAA+TGG - chr1.1:14624593-14624612 None:intergenic 25.0%
!! TTGTGAGCGTTATAAATTTT+GGG + chr1.1:14624738-14624757 MS.gene31841:intron 25.0%
!!! CTTGTTAACCATTTTTGATA+TGG + chr1.1:14624582-14624601 MS.gene31841:intron 25.0%
!!! GAATGAAATTTTGATTGAAG+GGG + chr1.1:14624622-14624641 MS.gene31841:intron 25.0%
AAAGAGATAGAGGTCAAATA+TGG - chr1.1:14624668-14624687 None:intergenic 30.0%
AAATAAAGTGAGAGAAAAGG+TGG + chr1.1:14624971-14624990 MS.gene31841:intron 30.0%
AAGAGATAGAGGTCAAATAT+GGG - chr1.1:14624667-14624686 None:intergenic 30.0%
AATAAAGTGAGAGAAAAGGT+GGG + chr1.1:14624972-14624991 MS.gene31841:intron 30.0%
ATTTACTATGGACGATGAAT+AGG + chr1.1:14625651-14625670 MS.gene31841:CDS 30.0%
CAAAACTGACGTATTTAATG+GGG - chr1.1:14625015-14625034 None:intergenic 30.0%
TGTGTCTAAATTTAAGTGCA+AGG + chr1.1:14625568-14625587 MS.gene31841:CDS 30.0%
TTGCTTCTATTAGATTCTCA+AGG + chr1.1:14624507-14624526 MS.gene31841:intron 30.0%
TTGTAAAACATTGTAGCTGA+AGG + chr1.1:14625371-14625390 MS.gene31841:intron 30.0%
! AATAATCACTGATGATGCTA+AGG + chr1.1:14625529-14625548 MS.gene31841:CDS 30.0%
! CTTGTGAGCGTTATAAATTT+TGG + chr1.1:14624737-14624756 MS.gene31841:intron 30.0%
! TCAGCTACAATGTTTTACAA+TGG - chr1.1:14625371-14625390 None:intergenic 30.0%
! TGTGAGCGTTATAAATTTTG+GGG + chr1.1:14624739-14624758 MS.gene31841:intron 30.0%
!! TTTTGAGTGTGAATCGAATT+CGG - chr1.1:14624767-14624786 None:intergenic 30.0%
ATTCTCTTGATGCTCTACAT+TGG - chr1.1:14625618-14625637 None:intergenic 35.0%
CAAAAACTATCGCAAGAACA+TGG + chr1.1:14625715-14625734 MS.gene31841:CDS 35.0%
GAGCAGATTTGTGTATACAA+TGG + chr1.1:14624818-14624837 MS.gene31841:intron 35.0%
TCATCAGTGATTATTGTGTC+TGG - chr1.1:14625524-14625543 None:intergenic 35.0%
TCTAATAGTGTGAAGCTAAC+TGG + chr1.1:14625058-14625077 MS.gene31841:intron 35.0%
TCTCAAGGAATTTATTGCCT+CGG + chr1.1:14624522-14624541 MS.gene31841:intron 35.0%
TTTCCGCTGATGATTTACTA+TGG + chr1.1:14625639-14625658 MS.gene31841:CDS 35.0%
! GCTACAATGTTTTACAATGG+AGG - chr1.1:14625368-14625387 None:intergenic 35.0%
! GTAGTCACATTTTTACAAGC+TGG + chr1.1:14625181-14625200 MS.gene31841:intron 35.0%
! TGTTCTTGCGATAGTTTTTG+AGG - chr1.1:14625715-14625734 None:intergenic 35.0%
AACTATCGCAAGAACATGGA+TGG + chr1.1:14625719-14625738 MS.gene31841:CDS 40.0%
ACTATCGCAAGAACATGGAT+GGG + chr1.1:14625720-14625739 MS.gene31841:CDS 40.0%
ACTATGGACGATGAATAGGT+TGG + chr1.1:14625655-14625674 MS.gene31841:CDS 40.0%
CAACATTCAACTCCAGCAAT+GGG + chr1.1:14625437-14625456 MS.gene31841:CDS 40.0%
CAGATCTTGATTGTGTCGAA+CGG - chr1.1:14624306-14624325 None:intergenic 40.0%
CGTCCATAGTAAATCATCAG+CGG - chr1.1:14625645-14625664 None:intergenic 40.0%
CTATGGACGATGAATAGGTT+GGG + chr1.1:14625656-14625675 MS.gene31841:CDS 40.0%
CTCAAGGAATTTATTGCCTC+GGG + chr1.1:14624523-14624542 MS.gene31841:intron 40.0%
GGGCAAGAATGATCTAACTT+GGG + chr1.1:14624543-14624562 MS.gene31841:intron 40.0%
TCAACGTTGGTCATCATCAT+TGG - chr1.1:14625771-14625790 None:intergenic 40.0%
TCAGTGATTATTGTGTCTGG+TGG - chr1.1:14625521-14625540 None:intergenic 40.0%
! GAACGGTTGTTGTTAGATGA+GGG - chr1.1:14624289-14624308 None:intergenic 40.0%
ACCAACAACAACACTGGCAT+TGG + chr1.1:14625404-14625423 MS.gene31841:CDS 45.0%
ACTCTTGTCACGTGACTGAT+GGG - chr1.1:14625482-14625501 None:intergenic 45.0%
AGGAACATGTTCGGTCTCTT+GGG + chr1.1:14625684-14625703 MS.gene31841:CDS 45.0%
AGTTAGATCATTCTTGCCCG+AGG - chr1.1:14624542-14624561 None:intergenic 45.0%
CCAACATTCAACTCCAGCAA+TGG + chr1.1:14625436-14625455 MS.gene31841:CDS 45.0%
CCATTGCTGGAGTTGAATGT+TGG - chr1.1:14625439-14625458 None:intergenic 45.0%
CGGGCAAGAATGATCTAACT+TGG + chr1.1:14624542-14624561 MS.gene31841:intron 45.0%
CGTTCGACACAATCAAGATC+TGG + chr1.1:14624304-14624323 MS.gene31841:CDS 45.0%
TAAGTGCAAGGTCACAAGTG+AGG + chr1.1:14625580-14625599 MS.gene31841:CDS 45.0%
! AATGCCAGTGTTGTTGTTGG+TGG - chr1.1:14625405-14625424 None:intergenic 45.0%
! CGAACGGTTGTTGTTAGATG+AGG - chr1.1:14624290-14624309 None:intergenic 45.0%
! GATGAATAGGTTGGGCTTTG+AGG + chr1.1:14625664-14625683 MS.gene31841:CDS 45.0%
! TCCAATGCCAGTGTTGTTGT+TGG - chr1.1:14625408-14625427 None:intergenic 45.0%
!! GATGCTCTACATTGGCTTGT+TGG - chr1.1:14625610-14625629 None:intergenic 45.0%
!! TGGGCTTTGAGGAACATGTT+CGG + chr1.1:14625675-14625694 MS.gene31841:CDS 45.0%
!! TTAATTATAATAATTAATTC+AGG + chr1.1:14624918-14624937 MS.gene31841:intron 5.0%
GACTCTTGTCACGTGACTGA+TGG - chr1.1:14625483-14625502 None:intergenic 50.0%
GAGGAACATGTTCGGTCTCT+TGG + chr1.1:14625683-14625702 MS.gene31841:CDS 50.0%
ACCGCCACCAACAACAACAC+TGG + chr1.1:14625398-14625417 MS.gene31841:CDS 55.0%
ATGGATGGGTCAGGTGCTAG+TGG + chr1.1:14625734-14625753 MS.gene31841:CDS 55.0%
CGCAAGAACATGGATGGGTC+AGG + chr1.1:14625725-14625744 MS.gene31841:CDS 55.0%
TTCAACTCCAGCAATGGGCG+AGG + chr1.1:14625442-14625461 MS.gene31841:CDS 55.0%
! GCCAGTGTTGTTGTTGGTGG+CGG - chr1.1:14625402-14625421 None:intergenic 55.0%
TGCACTTCCTCGCCCATTGC+TGG - chr1.1:14625452-14625471 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 14624271 14625790 14624271 ID=MS.gene31841
chr1.1 mRNA 14624271 14625790 14624271 ID=MS.gene31841.t1;Parent=MS.gene31841
chr1.1 exon 14624271 14624325 14624271 ID=MS.gene31841.t1.exon1;Parent=MS.gene31841.t1
chr1.1 CDS 14624271 14624325 14624271 ID=cds.MS.gene31841.t1;Parent=MS.gene31841.t1
chr1.1 exon 14625387 14625790 14625387 ID=MS.gene31841.t1.exon2;Parent=MS.gene31841.t1
chr1.1 CDS 14625387 14625790 14625387 ID=cds.MS.gene31841.t1;Parent=MS.gene31841.t1
Gene Sequence

>MS.gene31841

ATGGAGCGTGGAAGTCCCTCATCTAACAACAACCGTTCGACACAATCAAGATCTGGTAAGCACTCTAAACAAAAAAAATTACACATTTGTAAATCTTTATAATGAGTTGAGATCCTCGTATTTTAATTTTTTTACTTTGTAAAATATTGAATATGGTATCTAATTTCATAGTAGATCTTATTGTTTTAATATTTTTCACTAGATTTTATTTGATTTTTTGTATTTTTTCCCCTTAATTGCTTCTATTAGATTCTCAAGGAATTTATTGCCTCGGGCAAGAATGATCTAACTTGGGATATGATTGTGCATCACTTGTTAACCATTTTTGATATGGGAAAATACTTAGTTTTTGAATGAAATTTTGATTGAAGGGGATGACAAAAAAATATGTATCCCATATTTGACCTCTATCTCTTTTACTTTATTTTATATATACCTTTAATATTTTTAATTTATTATTTTAACGCTTGTGAGCGTTATAAATTTTGGGGTACCGAATTCGATTCACACTCAAAAAGATTCGAGATCTTGAAATTAATTTTGTTCGGAGCAGATTTGTGTATACAATGGTGAGAAAAACAACTTTGCTAAGATTTGGATTTTTTAATATTATATATTATTGTATTTAAAACTATTGTGTTATAATATTAATTATAATAATTAATTCAGGTTACACAAAAATGCAATTATCGTGTGAAAGAAATAAAGTGAGAGAAAAGGTGGGTGAAAAAATAAATAAGTCCCCATTAAATACGTCAGTTTTGTTACAAATTTCATTGTTGATCTATCTAATAGTGTGAAGCTAACTGGCATATACACAGATAAAGAAAATGTTTGTATCTTAGTTGTAATAGCTTCATAGTTCTCGTTTCTATATGTAATGATTCTACTAACATCAAGTGATGTATGTGTAGTCACATTTTTACAAGCTGGTTTGCTACCTTCGATTAAAAAGAAGAAAAGAAATTATACGTTACATTTTTTGTCTAAGTGAAATTTTGTTAAAAGTTAAAAAAAAAAAAAAAAAAATTAATTTTCTCTTGTACCAAATCTTTTAATTTTGAGGAGCTAAAATTGATTTATTTTTTCTTTTACCTCCATTGTAAAACATTGTAGCTGAAGGAAACACCGCCACCAACAACAACACTGGCATTGGAACTGAGAACCAACATTCAACTCCAGCAATGGGCGAGGAAGTGCATGTGATTCCCATCAGTCACGTGACAAGAGTCATGCAGAATGTTCTTCCACCAGACACAATAATCACTGATGATGCTAAGGAGTTAATGCAACTGTTTGTGTCTAAATTTAAGTGCAAGGTCACAAGTGAGGCTTTCCAACAAGCCAATGTAGAGCATCAAGAGAATGTTTCCGCTGATGATTTACTATGGACGATGAATAGGTTGGGCTTTGAGGAACATGTTCGGTCTCTTGGGAAATACCTCAAAAACTATCGCAAGAACATGGATGGGTCAGGTGCTAGTGGCTCAAACAATGCCAATGATGATGACCAACGTTGA
Protein sequence

>MS.gene31841.t1

MERGSPSSNNNRSTQSRSAEGNTATNNNTGIGTENQHSTPAMGEEVHVIPISHVTRVMQNVLPPDTIITDDAKELMQLFVSKFKCKVTSEAFQQANVEHQENVSADDLLWTMNRLGFEEHVRSLGKYLKNYRKNMDGSGASGSNNANDDDQR