Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31886.t1 | KEH40804.1 | 81.8 | 66 | 12 | 0 | 1 | 66 | 338 | 403 | 1.90E-23 | 117.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31886.t1 | A0A072VFJ7 | 81.8 | 66 | 12 | 0 | 1 | 66 | 338 | 403 | 1.4e-23 | 117.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene31886.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31886.t1 | MTR_1g035570 | 81.818 | 66 | 12 | 0 | 1 | 66 | 338 | 403 | 3.50e-32 | 114 |
| MS.gene31886.t1 | MTR_1g035490 | 74.576 | 59 | 15 | 0 | 1 | 59 | 272 | 330 | 4.81e-28 | 102 |
| MS.gene31886.t1 | MTR_1g035620 | 56.364 | 55 | 22 | 1 | 2 | 56 | 356 | 408 | 1.57e-13 | 63.5 |
| MS.gene31886.t1 | MTR_1g035520 | 48.276 | 58 | 28 | 2 | 1 | 57 | 327 | 383 | 9.15e-13 | 61.2 |
| MS.gene31886.t1 | MTR_6g061070 | 47.368 | 57 | 30 | 0 | 1 | 57 | 414 | 470 | 1.14e-11 | 58.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 12 sgRNAs with CRISPR-Local
Find 10 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTTGTTCTATTGTTCTTTA+AGG | 0.164818 | 1.2:-19854445 | None:intergenic |
| ACGATAGTATTCATGGTTAT+AGG | 0.290213 | 1.2:-19854597 | MS.gene31886:CDS |
| AATTATTACGATAGTATTCA+TGG | 0.354510 | 1.2:-19854604 | MS.gene31886:CDS |
| GTGTTTGATGTTTCAATTGT+TGG | 0.364550 | 1.2:-19854484 | MS.gene31886:CDS |
| AAAGAATGCAAAGTGGAGAT+TGG | 0.446068 | 1.2:-19854547 | MS.gene31886:CDS |
| GTTATAGGTTCTCTGAAGGT+TGG | 0.464034 | 1.2:-19854582 | MS.gene31886:CDS |
| AACAATAGAACAAACAGCTA+AGG | 0.501698 | 1.2:+19854453 | None:intergenic |
| ACAATAGAACAAACAGCTAA+GGG | 0.530051 | 1.2:+19854454 | None:intergenic |
| CATGGTTATAGGTTCTCTGA+AGG | 0.540492 | 1.2:-19854586 | MS.gene31886:CDS |
| ATTCATAAAAGAATGCAAAG+TGG | 0.613826 | 1.2:-19854554 | MS.gene31886:CDS |
| CAATAGAACAAACAGCTAAG+GGG | 0.667474 | 1.2:+19854455 | None:intergenic |
| TCTCTGGAGTTGAAAATGGG+GGG | 0.680996 | 1.2:-19854649 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTATTACGATAGTATTCA+TGG | - | chr1.2:19854477-19854496 | MS.gene31886:CDS | 20.0% |
| ! | ATTCATAAAAGAATGCAAAG+TGG | - | chr1.2:19854527-19854546 | MS.gene31886:CDS | 25.0% |
| AACAATAGAACAAACAGCTA+AGG | + | chr1.2:19854631-19854650 | None:intergenic | 30.0% | |
| ACAATAGAACAAACAGCTAA+GGG | + | chr1.2:19854630-19854649 | None:intergenic | 30.0% | |
| ACGATAGTATTCATGGTTAT+AGG | - | chr1.2:19854484-19854503 | MS.gene31886:CDS | 30.0% | |
| GTGTTTGATGTTTCAATTGT+TGG | - | chr1.2:19854597-19854616 | MS.gene31886:CDS | 30.0% | |
| AAAGAATGCAAAGTGGAGAT+TGG | - | chr1.2:19854534-19854553 | MS.gene31886:CDS | 35.0% | |
| CAATAGAACAAACAGCTAAG+GGG | + | chr1.2:19854629-19854648 | None:intergenic | 35.0% | |
| CATGGTTATAGGTTCTCTGA+AGG | - | chr1.2:19854495-19854514 | MS.gene31886:CDS | 40.0% | |
| ! | GTTATAGGTTCTCTGAAGGT+TGG | - | chr1.2:19854499-19854518 | MS.gene31886:CDS | 40.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 19854447 | 19854656 | 19854447 | ID=MS.gene31886 |
| chr1.2 | mRNA | 19854447 | 19854656 | 19854447 | ID=MS.gene31886.t1;Parent=MS.gene31886 |
| chr1.2 | exon | 19854447 | 19854656 | 19854447 | ID=MS.gene31886.t1.exon1;Parent=MS.gene31886.t1 |
| chr1.2 | CDS | 19854447 | 19854656 | 19854447 | ID=cds.MS.gene31886.t1;Parent=MS.gene31886.t1 |
| Gene Sequence |
| Protein sequence |
