Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31894.t1 | KEH40804.1 | 87.8 | 74 | 9 | 0 | 3 | 76 | 320 | 393 | 6.90E-30 | 139.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31894.t1 | A0A072VFJ7 | 87.8 | 74 | 9 | 0 | 3 | 76 | 320 | 393 | 5.0e-30 | 139.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene31894.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31894.t1 | MTR_1g035490 | 82.895 | 76 | 13 | 0 | 2 | 77 | 253 | 328 | 1.10e-39 | 133 |
| MS.gene31894.t1 | MTR_1g035570 | 85.526 | 76 | 11 | 0 | 2 | 77 | 319 | 394 | 2.03e-39 | 134 |
| MS.gene31894.t1 | MTR_1g035620 | 59.375 | 64 | 24 | 1 | 13 | 76 | 347 | 408 | 7.70e-18 | 75.9 |
| MS.gene31894.t1 | MTR_1g035520 | 44.156 | 77 | 41 | 1 | 3 | 77 | 307 | 383 | 5.86e-16 | 70.9 |
| MS.gene31894.t1 | MTR_6g061070 | 50.820 | 61 | 30 | 0 | 17 | 77 | 410 | 470 | 1.04e-14 | 67.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 16 sgRNAs with CRISPR-Local
Find 17 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAGAGGTTGGATAAAATTTA+TGG | 0.178729 | 1.1:-19802179 | MS.gene31894:CDS |
| GCCAAAGGAGTTTATGAAAA+TGG | 0.215569 | 1.1:-19802296 | MS.gene31894:CDS |
| AAATATTGTGGAGTTGAAAA+TGG | 0.256652 | 1.1:-19802263 | MS.gene31894:CDS |
| CCAAAGGAGTTTATGAAAAT+GGG | 0.408096 | 1.1:-19802295 | MS.gene31894:CDS |
| GAAGAATGCAAAGTGGAGAT+TGG | 0.433387 | 1.1:-19802157 | MS.gene31894:CDS |
| AATATTGTGGAGTTGAAAAT+GGG | 0.451536 | 1.1:-19802262 | MS.gene31894:CDS |
| CGTGGCTGTAGGTTCGGTAG+AGG | 0.492240 | 1.1:-19802196 | MS.gene31894:CDS |
| AACTATTACGAGAGTATTCG+TGG | 0.500607 | 1.1:-19802214 | MS.gene31894:CDS |
| AGTATTCGTGGCTGTAGGTT+CGG | 0.502330 | 1.1:-19802202 | MS.gene31894:CDS |
| GGGATATCATGAAAATATTG+TGG | 0.512948 | 1.1:-19802275 | MS.gene31894:CDS |
| GCTGTAGGTTCGGTAGAGGT+TGG | 0.515331 | 1.1:-19802192 | MS.gene31894:CDS |
| GTATCTGCAGTGTGTGCCAA+AGG | 0.603540 | 1.1:-19802311 | MS.gene31894:intron |
| ACGAGAGTATTCGTGGCTGT+AGG | 0.618825 | 1.1:-19802207 | MS.gene31894:CDS |
| ATTTATGGAAGAATGCAAAG+TGG | 0.635124 | 1.1:-19802164 | MS.gene31894:CDS |
| TGAAAATGGGGAGAAAGTCA+TGG | 0.640455 | 1.1:-19802249 | MS.gene31894:CDS |
| ATATTGTGGAGTTGAAAATG+GGG | 0.692849 | 1.1:-19802261 | MS.gene31894:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAATATTGTGGAGTTGAAAA+TGG | - | chr1.1:19802202-19802221 | MS.gene31894:CDS | 25.0% |
| ! | AATATTGTGGAGTTGAAAAT+GGG | - | chr1.1:19802203-19802222 | MS.gene31894:CDS | 25.0% |
| ! | TAGAGGTTGGATAAAATTTA+TGG | - | chr1.1:19802286-19802305 | MS.gene31894:CDS | 25.0% |
| ATATTGTGGAGTTGAAAATG+GGG | - | chr1.1:19802204-19802223 | MS.gene31894:CDS | 30.0% | |
| ATTTATGGAAGAATGCAAAG+TGG | - | chr1.1:19802301-19802320 | MS.gene31894:CDS | 30.0% | |
| CCAAAGGAGTTTATGAAAAT+GGG | - | chr1.1:19802170-19802189 | MS.gene31894:CDS | 30.0% | |
| GGGATATCATGAAAATATTG+TGG | - | chr1.1:19802190-19802209 | MS.gene31894:CDS | 30.0% | |
| GCCAAAGGAGTTTATGAAAA+TGG | - | chr1.1:19802169-19802188 | MS.gene31894:CDS | 35.0% | |
| ! | CCCATTTTCATAAACTCCTT+TGG | + | chr1.1:19802173-19802192 | None:intergenic | 35.0% |
| !! | AACTATTACGAGAGTATTCG+TGG | - | chr1.1:19802251-19802270 | MS.gene31894:CDS | 35.0% |
| GAAGAATGCAAAGTGGAGAT+TGG | - | chr1.1:19802308-19802327 | MS.gene31894:intron | 40.0% | |
| TGAAAATGGGGAGAAAGTCA+TGG | - | chr1.1:19802216-19802235 | MS.gene31894:CDS | 40.0% | |
| AGTATTCGTGGCTGTAGGTT+CGG | - | chr1.1:19802263-19802282 | MS.gene31894:CDS | 45.0% | |
| GTATCTGCAGTGTGTGCCAA+AGG | - | chr1.1:19802154-19802173 | MS.gene31894:CDS | 50.0% | |
| !! | ACGAGAGTATTCGTGGCTGT+AGG | - | chr1.1:19802258-19802277 | MS.gene31894:CDS | 50.0% |
| ! | GCTGTAGGTTCGGTAGAGGT+TGG | - | chr1.1:19802273-19802292 | MS.gene31894:CDS | 55.0% |
| ! | CGTGGCTGTAGGTTCGGTAG+AGG | - | chr1.1:19802269-19802288 | MS.gene31894:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 19802093 | 19802394 | 19802093 | ID=MS.gene31894 |
| chr1.1 | mRNA | 19802093 | 19802394 | 19802093 | ID=MS.gene31894.t1;Parent=MS.gene31894 |
| chr1.1 | exon | 19802392 | 19802394 | 19802392 | ID=MS.gene31894.t1.exon1;Parent=MS.gene31894.t1 |
| chr1.1 | CDS | 19802392 | 19802394 | 19802392 | ID=cds.MS.gene31894.t1;Parent=MS.gene31894.t1 |
| chr1.1 | exon | 19802093 | 19802323 | 19802093 | ID=MS.gene31894.t1.exon2;Parent=MS.gene31894.t1 |
| chr1.1 | CDS | 19802093 | 19802323 | 19802093 | ID=cds.MS.gene31894.t1;Parent=MS.gene31894.t1 |
| Gene Sequence |
| Protein sequence |
