AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene31932

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31932.t1 MTR_4g088850 97.802 91 2 0 1 91 43 133 1.09e-60 181
MS.gene31932.t1 MTR_4g088850 97.143 70 2 0 1 70 43 112 7.63e-44 139
MS.gene31932.t1 MTR_4g098500 56.338 71 31 0 1 71 47 117 3.68e-23 86.3
MS.gene31932.t1 MTR_4g098500 56.338 71 31 0 1 71 73 143 9.57e-23 85.9
MS.gene31932.t1 MTR_2g047838 42.353 85 49 0 1 85 47 131 1.18e-22 86.3
MS.gene31932.t1 MTR_8g089310 50.000 76 38 0 1 76 44 119 1.61e-21 83.2
MS.gene31932.t1 MTR_8g089310 50.000 76 38 0 1 76 57 132 2.01e-21 83.6
MS.gene31932.t1 MTR_8g089310 50.000 76 38 0 1 76 91 166 4.41e-21 83.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31932.t1 AT4G21610 75.309 81 20 0 1 81 61 141 1.78e-39 128
MS.gene31932.t1 AT4G21610 75.362 69 17 0 1 69 61 129 9.75e-31 105
MS.gene31932.t1 AT1G32540 54.930 71 32 0 1 71 107 177 1.20e-22 86.7
MS.gene31932.t1 AT1G32540 54.930 71 32 0 1 71 74 144 1.65e-22 85.5
MS.gene31932.t1 AT1G32540 54.930 71 32 0 1 71 74 144 1.65e-22 85.5
MS.gene31932.t1 AT1G32540 57.576 66 28 0 1 66 74 139 7.39e-21 81.3
MS.gene31932.t1 AT4G20380 44.186 86 39 1 1 77 51 136 1.68e-19 78.6
MS.gene31932.t1 AT4G20380 44.186 86 39 1 1 77 51 136 1.68e-19 78.6
MS.gene31932.t1 AT4G20380 44.186 86 39 1 1 77 46 131 1.69e-19 78.2
MS.gene31932.t1 AT4G20380 44.186 86 39 1 1 77 46 131 1.69e-19 78.2
MS.gene31932.t1 AT4G20380 44.186 86 39 1 1 77 46 131 1.69e-19 78.2
MS.gene31932.t1 AT4G20380 44.186 86 39 1 1 77 46 131 1.69e-19 78.2
MS.gene31932.t1 AT4G20380 44.186 86 39 1 1 77 46 131 1.69e-19 78.2
MS.gene31932.t1 AT4G20380 44.872 78 34 1 1 69 46 123 1.43e-17 72.0
MS.gene31932.t1 AT4G20380 37.662 77 45 1 1 74 5 81 1.28e-11 56.6
MS.gene31932.t1 AT4G20380 42.424 66 35 1 1 63 5 70 4.21e-11 57.0

Find 32 sgRNAs with CRISPR-Local

Find 216 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCCGGTTTGTGACAGAAAT+CGG 0.319178 4.1:-20974529 MS.gene31932:CDS
AGGCCGCTAACAACCAGATT+TGG 0.328376 4.1:+20973991 None:intergenic
GCATGAACATTTGACATGTT+TGG 0.343047 4.1:+20976787 None:intergenic
TTCCTTCGTCACAGCTGATC+AGG 0.397948 4.1:-20974629 MS.gene31932:intron
GGCCGCTAACAACCAGATTT+GGG 0.413926 4.1:+20973992 None:intergenic
AAACCAACTCCTCCCAAATC+TGG 0.417656 4.1:-20974004 MS.gene31932:CDS
GCCGGTTTGTGACAGAAATC+GGG 0.420532 4.1:-20974528 MS.gene31932:CDS
CATACGGTGCTCCACAAGTT+AGG 0.427518 4.1:-20974561 MS.gene31932:CDS
CTAATACAATGTTGACTGTC+TGG 0.430906 4.1:+20976762 None:intergenic
CAACCAGATTTGGGAGGAGT+TGG 0.440236 4.1:+20974001 None:intergenic
CCCCGATTTCTGTCACAAAC+CGG 0.464843 4.1:+20974527 None:intergenic
ACAGTCAACATTGTATTAGA+AGG 0.509196 4.1:-20976758 MS.gene31932:intron
CATTTGACATGTTTGGCACC+TGG 0.517148 4.1:+20976794 None:intergenic
CGCCTGCTTTCGTATCCACC+AGG 0.522323 4.1:-20976812 MS.gene31932:CDS
AAGTTAGGTGTTCATCATGC+CGG 0.527912 4.1:-20974546 MS.gene31932:CDS
CAGACAACAAGCGGCCTCCA+TGG 0.537061 4.1:-20974042 MS.gene31932:CDS
CTAACTTGTGGAGCACCGTA+TGG 0.541463 4.1:+20974562 None:intergenic
CACCTGGTGGATACGAAAGC+AGG 0.543193 4.1:+20976810 None:intergenic
CTCCCAAATCTGGTTGTTAG+CGG 0.560296 4.1:-20973994 None:intergenic
TTGTGCAGGCAGACAACAAG+CGG 0.571247 4.1:-20974051 MS.gene31932:intron
TAACTTGTGGAGCACCGTAT+GGG 0.577193 4.1:+20974563 None:intergenic
TTACTTTGTTGTACAGACCA+TGG 0.588700 4.1:+20974025 None:intergenic
TTCGTCACAGCTGATCAGGT+TGG 0.596338 4.1:-20974625 MS.gene31932:intron
CAGGTTGGACAAGTTAAGTG+TGG 0.602861 4.1:-20974610 MS.gene31932:CDS
CATGATGAACACCTAACTTG+TGG 0.604697 4.1:+20974550 None:intergenic
AGGTTGGACAAGTTAAGTGT+GGG 0.628125 4.1:-20974609 MS.gene31932:CDS
GTATTGCTAATGTGCCCATA+CGG 0.638828 4.1:-20974577 MS.gene31932:CDS
TTGACATGTTTGGCACCTGG+TGG 0.642814 4.1:+20976797 None:intergenic
CCGGTTTGTGACAGAAATCG+GGG 0.660382 4.1:-20974527 MS.gene31932:intron
CGCTAACAACCAGATTTGGG+AGG 0.672253 4.1:+20973995 None:intergenic
CTGGTGGATACGAAAGCAGG+CGG 0.684251 4.1:+20976813 None:intergenic
CTTTGTTGTACAGACCATGG+AGG 0.723748 4.1:+20974028 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTATTGATTTTATATTGT+TGG - chr4.1:20976709-20976728 MS.gene31932:intron 10.0%
!! AAAATGTCTAATTGAAAATT+TGG + chr4.1:20976022-20976041 None:intergenic 15.0%
!!! TTTTGACTTAAAAAATTAGA+AGG + chr4.1:20976374-20976393 None:intergenic 15.0%
!! ACATATTACATATTGTTCTA+AGG - chr4.1:20976428-20976447 MS.gene31932:intron 20.0%
!! AGTAACATTTCTAACAAAAA+AGG - chr4.1:20975913-20975932 MS.gene31932:intron 20.0%
!! ATATTCAACATTGTATTAGA+AGG - chr4.1:20975688-20975707 MS.gene31932:intron 20.0%
!! GAAAATTTCATTAACATAAC+AGG + chr4.1:20975622-20975641 None:intergenic 20.0%
!! TTCATAAACAATTACATGAT+TGG - chr4.1:20975812-20975831 MS.gene31932:intron 20.0%
!! TTCATATTTGAAAATTGTGT+CGG + chr4.1:20975124-20975143 None:intergenic 20.0%
!!! TTCAATTAGACATTTTAGTA+TGG - chr4.1:20976026-20976045 MS.gene31932:intron 20.0%
! AACAAATTTGCAGTTTAGAT+AGG - chr4.1:20974655-20974674 MS.gene31932:intron 25.0%
! AACTTAAAATGCAAGTTTCA+TGG + chr4.1:20975760-20975779 None:intergenic 25.0%
! ATAATGACCAAATATTTGCT+TGG - chr4.1:20974965-20974984 MS.gene31932:intron 25.0%
! ATCAAAAGTTGAAGATTACA+TGG - chr4.1:20975196-20975215 MS.gene31932:intron 25.0%
! ATTTGTTCAAAGTACAAACA+AGG + chr4.1:20974642-20974661 None:intergenic 25.0%
! CTTTAAATTATCCAAGTGTA+GGG - chr4.1:20976599-20976618 MS.gene31932:intron 25.0%
! TACATAAATCAACTTGCAAT+TGG + chr4.1:20976409-20976428 None:intergenic 25.0%
! TACTTGTACAAAATATGACA+TGG - chr4.1:20976137-20976156 MS.gene31932:intron 25.0%
! TCAAAAGTTGAAGATTACAT+GGG - chr4.1:20975197-20975216 MS.gene31932:intron 25.0%
! TCATCAAATTCAAATGTGAT+TGG + chr4.1:20975984-20976003 None:intergenic 25.0%
! TTTAAATTATCCAAGTGTAG+GGG - chr4.1:20976600-20976619 MS.gene31932:intron 25.0%
! TTTGTTCAAAGTACAAACAA+GGG + chr4.1:20974641-20974660 None:intergenic 25.0%
!! ATGAAATTTTCAATGCTCTA+CGG - chr4.1:20974446-20974465 MS.gene31932:intron 25.0%
!!! TTTCTAAAAGCTAATTTTCG+AGG + chr4.1:20974185-20974204 None:intergenic 25.0%
AACAACTATTTCAACATCGA+TGG - chr4.1:20974817-20974836 MS.gene31932:intron 30.0%
AACTTCCTGGTCATTATAAA+AGG - chr4.1:20975936-20975955 MS.gene31932:intron 30.0%
ACAGTCAACATTGTATTAGA+AGG - chr4.1:20974072-20974091 MS.gene31932:intron 30.0%
ACTTCCTGGTCATTATAAAA+GGG - chr4.1:20975937-20975956 MS.gene31932:intron 30.0%
AGACATGTTCATGAGAAAAA+AGG + chr4.1:20974106-20974125 None:intergenic 30.0%
AGCTAACATGTATAAGATCA+TGG - chr4.1:20975473-20975492 MS.gene31932:intron 30.0%
CAGGAAATTTGATTACAATC+AGG + chr4.1:20975603-20975622 None:intergenic 30.0%
CCTACACTTGGATAATTTAA+AGG + chr4.1:20976601-20976620 None:intergenic 30.0%
CCTTTAAATTATCCAAGTGT+AGG - chr4.1:20976598-20976617 MS.gene31932:intron 30.0%
CTCAAGTTTATATCTCACAT+TGG - chr4.1:20974569-20974588 MS.gene31932:CDS 30.0%
TAGTTGATCTACGAAGATTT+GGG + chr4.1:20974353-20974372 None:intergenic 30.0%
TCTTCAGATTGAATGAATAG+AGG + chr4.1:20976678-20976697 None:intergenic 30.0%
TGAGGATATCAGTGTTTAAA+TGG + chr4.1:20976753-20976772 None:intergenic 30.0%
TGCAGCTTCAATTTCATTAT+GGG + chr4.1:20975235-20975254 None:intergenic 30.0%
TGCGATTCAGTTCAATAAAT+CGG + chr4.1:20974426-20974445 None:intergenic 30.0%
TTAAATTATCCAAGTGTAGG+GGG - chr4.1:20976601-20976620 MS.gene31932:intron 30.0%
TTGCAGCTTCAATTTCATTA+TGG + chr4.1:20975236-20975255 None:intergenic 30.0%
TTGTTCAAAGTACAAACAAG+GGG + chr4.1:20974640-20974659 None:intergenic 30.0%
! AAACTATCCTCTTTTGTCAA+TGG + chr4.1:20975450-20975469 None:intergenic 30.0%
! ATTCAAATGTGATTGGTCAT+TGG + chr4.1:20975977-20975996 None:intergenic 30.0%
! TGAAATTTTCAATGCTCTAC+GGG - chr4.1:20974447-20974466 MS.gene31932:intron 30.0%
!! AGTTCAGGTTTTTATACATC+TGG - chr4.1:20975776-20975795 MS.gene31932:intron 30.0%
!!! AGTCTAGTTTTCTCTTTGTA+CGG - chr4.1:20976536-20976555 MS.gene31932:intron 30.0%
!!! GAAACTTGCATTTTAAGTTC+AGG - chr4.1:20975761-20975780 MS.gene31932:intron 30.0%
AAAGAGTGGTTAAGTTCCAT+TGG - chr4.1:20974512-20974531 MS.gene31932:intron 35.0%
AAAGTTGAAGATTACATGGG+AGG - chr4.1:20975200-20975219 MS.gene31932:intron 35.0%
AACAACTCGTTGATCATAGT+TGG + chr4.1:20975172-20975191 None:intergenic 35.0%
AACTATGATCAACGAGTTGT+TGG - chr4.1:20975171-20975190 MS.gene31932:intron 35.0%
AAGTTTGACACTTGATGTAG+CGG - chr4.1:20976468-20976487 MS.gene31932:intron 35.0%
AATGATTGACCAAGAACTCA+TGG - chr4.1:20975389-20975408 MS.gene31932:intron 35.0%
ACAATCAGGCGTTAAACAAA+AGG + chr4.1:20975589-20975608 None:intergenic 35.0%
ACAGGCTACTCCTAAAATTA+GGG + chr4.1:20975034-20975053 None:intergenic 35.0%
ACATAGCGTATATGCATTCT+TGG + chr4.1:20976061-20976080 None:intergenic 35.0%
ACATTGTTTGGATATTGCTG+TGG + chr4.1:20975090-20975109 None:intergenic 35.0%
ACTACGCTCATGTAATTAGA+AGG - chr4.1:20975505-20975524 MS.gene31932:intron 35.0%
AGAAGATGAGAACTATAACC+AGG - chr4.1:20974993-20975012 MS.gene31932:intron 35.0%
CATTGTTTGGATATTGCTGT+GGG + chr4.1:20975089-20975108 None:intergenic 35.0%
CGATTCAGTTCAATAAATCG+GGG + chr4.1:20974424-20974443 None:intergenic 35.0%
CTAACAAAAAAGGAACTTCC+TGG - chr4.1:20975923-20975942 MS.gene31932:intron 35.0%
CTACAACAGATGAACCATAT+CGG - chr4.1:20974370-20974389 MS.gene31932:intron 35.0%
GCAAATGATTGGAATGAGAA+TGG + chr4.1:20974767-20974786 None:intergenic 35.0%
GCATGAACATTTGACATGTT+TGG + chr4.1:20974046-20974065 None:intergenic 35.0%
GCGATTCAGTTCAATAAATC+GGG + chr4.1:20974425-20974444 None:intergenic 35.0%
GGTTATAGTTCTCATCTTCT+GGG + chr4.1:20974993-20975012 None:intergenic 35.0%
GTAGTTGATCTACGAAGATT+TGG + chr4.1:20974354-20974373 None:intergenic 35.0%
GTGAGATATAAACTTGAGAG+AGG + chr4.1:20974567-20974586 None:intergenic 35.0%
TACAGGCTACTCCTAAAATT+AGG + chr4.1:20975035-20975054 None:intergenic 35.0%
TATCATCTTGATCTTCGTCT+TGG + chr4.1:20975421-20975440 None:intergenic 35.0%
TCAATAAATCGGGGATTGTT+GGG + chr4.1:20974415-20974434 None:intergenic 35.0%
TCAGATTGAATGAATAGAGG+CGG + chr4.1:20976675-20976694 None:intergenic 35.0%
TGAAATAGTTGTTACCGTCT+AGG + chr4.1:20974810-20974829 None:intergenic 35.0%
TGGTTATAGTTCTCATCTTC+TGG + chr4.1:20974994-20975013 None:intergenic 35.0%
TTCAATAAATCGGGGATTGT+TGG + chr4.1:20974416-20974435 None:intergenic 35.0%
TTTATTTGAGAATCTCCAGC+TGG - chr4.1:20974157-20974176 MS.gene31932:intron 35.0%
! CATGGCATTATTCTTTTGCA+TGG - chr4.1:20976155-20976174 MS.gene31932:intron 35.0%
! CTAATACAATGTTGACTGTC+TGG + chr4.1:20974071-20974090 None:intergenic 35.0%
! GTATCCCTTTTATAATGACC+AGG + chr4.1:20975944-20975963 None:intergenic 35.0%
! TATCCTCATGATTTTTGTGC+AGG - chr4.1:20976765-20976784 MS.gene31932:CDS 35.0%
! TTACTTTGTTGTACAGACCA+TGG + chr4.1:20976808-20976827 None:intergenic 35.0%
!! TTGTATACTCAGCTTTAAGC+TGG + chr4.1:20975287-20975306 None:intergenic 35.0%
AAGTTAGGTGTTCATCATGC+CGG - chr4.1:20976284-20976303 MS.gene31932:intron 40.0%
AATAGTTGTTACCGTCTAGG+TGG + chr4.1:20974807-20974826 None:intergenic 40.0%
ACCTATTCCAATGACGTTCT+AGG - chr4.1:20974916-20974935 MS.gene31932:intron 40.0%
AGCACACAACTACCAATAAC+AGG + chr4.1:20975847-20975866 None:intergenic 40.0%
AGGTTGGACAAGTTAAGTGT+GGG - chr4.1:20976221-20976240 MS.gene31932:intron 40.0%
ATAGTTGGAGTAGAACTGAG+CGG + chr4.1:20975157-20975176 None:intergenic 40.0%
ATCGGATCATTCACCATTCT+TGG - chr4.1:20974388-20974407 MS.gene31932:intron 40.0%
ATCTACGAAGATTTGGGTAG+TGG + chr4.1:20974347-20974366 None:intergenic 40.0%
CAGATTGAATGAATAGAGGC+GGG + chr4.1:20976674-20976693 None:intergenic 40.0%
CATGATGAACACCTAACTTG+TGG + chr4.1:20976283-20976302 None:intergenic 40.0%
CCACTTTGACATAAAGTGGA+GGG + chr4.1:20974482-20974501 None:intergenic 40.0%
CCTCCACTTTATGTCAAAGT+GGG - chr4.1:20974480-20974499 MS.gene31932:intron 40.0%
CTGGGAACCAAGCAAATATT+TGG + chr4.1:20974975-20974994 None:intergenic 40.0%
GAATGGTGAATGATCCGATA+TGG + chr4.1:20974387-20974406 None:intergenic 40.0%
GTATTGCTAATGTGCCCATA+CGG - chr4.1:20976253-20976272 MS.gene31932:intron 40.0%
TATAACCCTGATACCATGTC+AGG - chr4.1:20974241-20974260 MS.gene31932:intron 40.0%
TCCTAGAACGTCATTGGAAT+AGG + chr4.1:20974920-20974939 None:intergenic 40.0%
TGTCCCACTTTGACATAAAG+TGG + chr4.1:20974486-20974505 None:intergenic 40.0%
TGTTTATGCCTCAAACAGAG+AGG - chr4.1:20974600-20974619 MS.gene31932:CDS 40.0%
! ACATAAAGTGGAGGGTGTAA+AGG + chr4.1:20974474-20974493 None:intergenic 40.0%
! ATCAACCTGGCCCTAATTTT+AGG - chr4.1:20975021-20975040 MS.gene31932:intron 40.0%
! CTATCCTCTTTTGTCAATGG+CGG + chr4.1:20975447-20975466 None:intergenic 40.0%
! TATCCTCTTTTGTCAATGGC+GGG + chr4.1:20975446-20975465 None:intergenic 40.0%
! TTGAAAATTGTGTCGGCGTT+GGG + chr4.1:20975117-20975136 None:intergenic 40.0%
! TTTGAAAATTGTGTCGGCGT+TGG + chr4.1:20975118-20975137 None:intergenic 40.0%
!! ATTCCAATGACGTTCTAGGA+CGG - chr4.1:20974920-20974939 MS.gene31932:intron 40.0%
!! CTTGAGAGCATTGAGCAATA+GGG + chr4.1:20974713-20974732 None:intergenic 40.0%
!! GTGGGACATTTTGAAAAGAG+TGG - chr4.1:20974498-20974517 MS.gene31932:intron 40.0%
!! TCATCAGCCATTTGCTGTTT+GGG + chr4.1:20974894-20974913 None:intergenic 40.0%
!! TTCATCAGCCATTTGCTGTT+TGG + chr4.1:20974895-20974914 None:intergenic 40.0%
AAACCAACTCCTCCCAAATC+TGG - chr4.1:20976826-20976845 MS.gene31932:CDS 45.0%
AATGGGGTGGTGCAAATGAT+TGG + chr4.1:20974778-20974797 None:intergenic 45.0%
ACAGCTTATGAGTTGCAGTG+CGG + chr4.1:20975545-20975564 None:intergenic 45.0%
ATCATCAATCCCCCTACACT+TGG + chr4.1:20976613-20976632 None:intergenic 45.0%
ATCTTCATCCAGTCCTGACA+TGG + chr4.1:20974257-20974276 None:intergenic 45.0%
ATTGAATGAATAGAGGCGGG+TGG + chr4.1:20976671-20976690 None:intergenic 45.0%
CATTTGACATGTTTGGCACC+TGG + chr4.1:20974039-20974058 None:intergenic 45.0%
CCCACTTTGACATAAAGTGG+AGG + chr4.1:20974483-20974502 None:intergenic 45.0%
CCCTCCACTTTATGTCAAAG+TGG - chr4.1:20974479-20974498 MS.gene31932:intron 45.0%
CGCTGAAGAGTTCAGTATTG+AGG + chr4.1:20974849-20974868 None:intergenic 45.0%
CGTCTAGGTGGTTCCAAAAT+GGG + chr4.1:20974795-20974814 None:intergenic 45.0%
CTACTCCTAAAATTAGGGCC+AGG + chr4.1:20975029-20975048 None:intergenic 45.0%
CTGCCTGCACAAAAATCATG+AGG + chr4.1:20976771-20976790 None:intergenic 45.0%
GCGTATATGCATTCTTGGAG+AGG + chr4.1:20976056-20976075 None:intergenic 45.0%
GTAGTGGTCCTTGAAAAGGA+CGG + chr4.1:20974331-20974350 None:intergenic 45.0%
GTCTAGGTGGTTCCAAAATG+GGG + chr4.1:20974794-20974813 None:intergenic 45.0%
GTGGGATATGATCATCGCTT+GGG + chr4.1:20975071-20975090 None:intergenic 45.0%
TAACCAGGATGACATCAACC+TGG - chr4.1:20975008-20975027 MS.gene31932:intron 45.0%
TAGTTGGAGTAGAACTGAGC+GGG + chr4.1:20975156-20975175 None:intergenic 45.0%
TGACTGACCCAAACAGCAAA+TGG - chr4.1:20974884-20974903 MS.gene31932:intron 45.0%
TGCCAGTCAAGTAGCCAAAA+TGG + chr4.1:20974946-20974965 None:intergenic 45.0%
TGGACGAACAGTCCTGTTAT+TGG - chr4.1:20975832-20975851 MS.gene31932:intron 45.0%
TGGGATATGATCATCGCTTG+GGG + chr4.1:20975070-20975089 None:intergenic 45.0%
TGTGGGATATGATCATCGCT+TGG + chr4.1:20975072-20975091 None:intergenic 45.0%
TTGAATGAATAGAGGCGGGT+GGG + chr4.1:20976670-20976689 None:intergenic 45.0%
TTGGTCAATCATTTCGCCAG+TGG + chr4.1:20975382-20975401 None:intergenic 45.0%
! ATAACTGGGGTGTTACCTTC+AGG + chr4.1:20974286-20974305 None:intergenic 45.0%
! ATTGGTAGTTGTGTGCTTGC+TGG - chr4.1:20975850-20975869 MS.gene31932:intron 45.0%
! CAGGTTGGACAAGTTAAGTG+TGG - chr4.1:20976220-20976239 MS.gene31932:intron 45.0%
! CTTTGTTGTACAGACCATGG+AGG + chr4.1:20976805-20976824 None:intergenic 45.0%
! TAACTGGGGTGTTACCTTCA+GGG + chr4.1:20974285-20974304 None:intergenic 45.0%
! TCATTTGCACCACCCCATTT+TGG - chr4.1:20974779-20974798 MS.gene31932:intron 45.0%
! TGGCTGGAGCTTTGATAACT+GGG + chr4.1:20974300-20974319 None:intergenic 45.0%
! TTGGGTAGTGGTCCTTGAAA+AGG + chr4.1:20974335-20974354 None:intergenic 45.0%
!! CTAATTTTCGAGGAACCAGC+TGG + chr4.1:20974175-20974194 None:intergenic 45.0%
!! GCTTGAGAGCATTGAGCAAT+AGG + chr4.1:20974714-20974733 None:intergenic 45.0%
!! TAACTTGTGGAGCACCGTAT+GGG + chr4.1:20976270-20976289 None:intergenic 45.0%
!! TGCCGGTTTGTGACAGAAAT+CGG - chr4.1:20976301-20976320 MS.gene31932:intron 45.0%
!!! CCATTTTGGAACCACCTAGA+CGG - chr4.1:20974793-20974812 MS.gene31932:intron 45.0%
AACCTGATCAGCTGTGACGA+AGG + chr4.1:20976206-20976225 None:intergenic 50.0%
AGACCCGCCATTGACAAAAG+AGG - chr4.1:20975440-20975459 MS.gene31932:intron 50.0%
CAACCAGATTTGGGAGGAGT+TGG + chr4.1:20976832-20976851 None:intergenic 50.0%
CAACTCAAGACCTGCTGCAA+TGG - chr4.1:20975314-20975333 MS.gene31932:intron 50.0%
CAGCTTATGAGTTGCAGTGC+GGG + chr4.1:20975544-20975563 None:intergenic 50.0%
CATACGGTGCTCCACAAGTT+AGG - chr4.1:20976269-20976288 MS.gene31932:intron 50.0%
CCAGTCCTGACATGGTATCA+GGG + chr4.1:20974249-20974268 None:intergenic 50.0%
CCCCGATTTCTGTCACAAAC+CGG + chr4.1:20976306-20976325 None:intergenic 50.0%
CCGTCTAGGTGGTTCCAAAA+TGG + chr4.1:20974796-20974815 None:intergenic 50.0%
GATGAAGATACGACCCCTGA+AGG - chr4.1:20974268-20974287 MS.gene31932:intron 50.0%
GCGTTGGGAACGACATTGTT+TGG + chr4.1:20975102-20975121 None:intergenic 50.0%
GGAATGAGAATGGAGCTGTC+AGG + chr4.1:20974757-20974776 None:intergenic 50.0%
GGGATTGTTGGGTCCAAGAA+TGG + chr4.1:20974404-20974423 None:intergenic 50.0%
GTATTTAGCCTCCTCAGCAG+TGG + chr4.1:20974545-20974564 None:intergenic 50.0%
TAGGTGGTTCCAAAATGGGG+TGG + chr4.1:20974791-20974810 None:intergenic 50.0%
TATGATCATCGCTTGGGGCT+AGG + chr4.1:20975065-20975084 None:intergenic 50.0%
TCAATGCTCTCAAGCCGACT+CGG - chr4.1:20974719-20974738 MS.gene31932:intron 50.0%
TCCAGTCCTGACATGGTATC+AGG + chr4.1:20974250-20974269 None:intergenic 50.0%
TGGCATGAGCCATGAGTTCT+TGG + chr4.1:20975401-20975420 None:intergenic 50.0%
TTCCTTCGTCACAGCTGATC+AGG - chr4.1:20976201-20976220 MS.gene31932:intron 50.0%
TTCGTCACAGCTGATCAGGT+TGG - chr4.1:20976205-20976224 MS.gene31932:intron 50.0%
TTGACATGTTTGGCACCTGG+TGG + chr4.1:20974036-20974055 None:intergenic 50.0%
TTGTGCAGGCAGACAACAAG+CGG - chr4.1:20976779-20976798 MS.gene31932:CDS 50.0%
! AACTGGGGTGTTACCTTCAG+GGG + chr4.1:20974284-20974303 None:intergenic 50.0%
! CCGGTTTGTGACAGAAATCG+GGG - chr4.1:20976303-20976322 MS.gene31932:intron 50.0%
! CTGGCTGGAGCTTTGATAAC+TGG + chr4.1:20974301-20974320 None:intergenic 50.0%
! GGCTGGAGCTTTGATAACTG+GGG + chr4.1:20974299-20974318 None:intergenic 50.0%
!! CTAACTTGTGGAGCACCGTA+TGG + chr4.1:20976271-20976290 None:intergenic 50.0%
!! GAGCATTGAGCAATAGGGTG+AGG + chr4.1:20974708-20974727 None:intergenic 50.0%
!! GCCGGTTTGTGACAGAAATC+GGG - chr4.1:20976302-20976321 MS.gene31932:intron 50.0%
!!! ACGTTCTAGGACGGCCATTT+TGG - chr4.1:20974929-20974948 MS.gene31932:intron 50.0%
!!! GGCCATTTTGGCTACTTGAC+TGG - chr4.1:20974941-20974960 MS.gene31932:intron 50.0%
AACGGCTTGTCCTGAGCCTT+CGG + chr4.1:20975346-20975365 None:intergenic 55.0%
AGTGGCGCGAAATGGGACAA+CGG + chr4.1:20975364-20975383 None:intergenic 55.0%
ATGGATACAGCCGAAGGCTC+AGG - chr4.1:20975333-20975352 MS.gene31932:intron 55.0%
ATTTCGCCAGTGGCGCGAAA+TGG + chr4.1:20975372-20975391 None:intergenic 55.0%
CCCTGATACCATGTCAGGAC+TGG - chr4.1:20974246-20974265 MS.gene31932:intron 55.0%
CCTCAAACAGAGAGGCTTGC+AGG - chr4.1:20974608-20974627 MS.gene31932:CDS 55.0%
CCTGCAAGCCTCTCTGTTTG+AGG + chr4.1:20974611-20974630 None:intergenic 55.0%
GCTGCAATGGATACAGCCGA+AGG - chr4.1:20975327-20975346 MS.gene31932:intron 55.0%
GTGGTCCTTGAAAAGGACGG+AGG + chr4.1:20974328-20974347 None:intergenic 55.0%
TCGGCTGTATCCATTGCAGC+AGG + chr4.1:20975327-20975346 None:intergenic 55.0%
TGAAAAGGACGGAGGTCTGC+TGG + chr4.1:20974320-20974339 None:intergenic 55.0%
TGGCCGTCCTAGAACGTCAT+TGG + chr4.1:20974926-20974945 None:intergenic 55.0%
TTGGAACTCCACCACTGCTG+AGG - chr4.1:20974531-20974550 MS.gene31932:CDS 55.0%
TTTAGCCTCCTCAGCAGTGG+TGG + chr4.1:20974542-20974561 None:intergenic 55.0%
TTTCGCCAGTGGCGCGAAAT+GGG + chr4.1:20975371-20975390 None:intergenic 55.0%
! ACTCTTCAGCGCACTGATGC+AGG - chr4.1:20974858-20974877 MS.gene31932:intron 55.0%
! CAATGCTCTCAAGCCGACTC+GGG - chr4.1:20974720-20974739 MS.gene31932:intron 55.0%
! CACCTGGTGGATACGAAAGC+AGG + chr4.1:20974023-20974042 None:intergenic 55.0%
! CAGCAGTGGTGGAGTTCCAA+TGG + chr4.1:20974531-20974550 None:intergenic 55.0%
! CTGGTGGATACGAAAGCAGG+CGG + chr4.1:20974020-20974039 None:intergenic 55.0%
! GCAGACCTCCGTCCTTTTCA+AGG - chr4.1:20974320-20974339 MS.gene31932:intron 55.0%
CAGACAACAAGCGGCCTCCA+TGG - chr4.1:20976788-20976807 MS.gene31932:CDS 60.0%
CGCCTGCTTTCGTATCCACC+AGG - chr4.1:20974018-20974037 MS.gene31932:CDS 60.0%
GAACTCCACCACTGCTGAGG+AGG - chr4.1:20974534-20974553 MS.gene31932:CDS 60.0%
GTTGTCCCATTTCGCGCCAC+TGG - chr4.1:20975363-20975382 MS.gene31932:intron 60.0%
TCATCGCTTGGGGCTAGGTG+TGG + chr4.1:20975060-20975079 None:intergenic 60.0%
TGGGGCTAGGTGTGGAGTAC+AGG + chr4.1:20975052-20975071 None:intergenic 60.0%
!! GGGCCAGGTTGATGTCATCC+TGG + chr4.1:20975014-20975033 None:intergenic 60.0%
AAGGACGGAGGTCTGCTGGC+TGG + chr4.1:20974316-20974335 None:intergenic 65.0%
GGCGTTCCAGTGACCCGAGT+CGG + chr4.1:20974736-20974755 None:intergenic 65.0%
!! CTCAAGCCGACTCGGGTCAC+TGG - chr4.1:20974727-20974746 MS.gene31932:intron 65.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 20973997 20976855 20973997 ID=MS.gene31932
chr4.1 mRNA 20973997 20976855 20973997 ID=MS.gene31932.t1;Parent=MS.gene31932
chr4.1 exon 20976759 20976855 20976759 ID=MS.gene31932.t1.exon1;Parent=MS.gene31932.t1
chr4.1 CDS 20976759 20976855 20976759 ID=cds.MS.gene31932.t1;Parent=MS.gene31932.t1
chr4.1 exon 20974528 20974637 20974528 ID=MS.gene31932.t1.exon2;Parent=MS.gene31932.t1
chr4.1 CDS 20974528 20974637 20974528 ID=cds.MS.gene31932.t1;Parent=MS.gene31932.t1
chr4.1 exon 20973997 20974065 20973997 ID=MS.gene31932.t1.exon3;Parent=MS.gene31932.t1
chr4.1 CDS 20973997 20974065 20973997 ID=cds.MS.gene31932.t1;Parent=MS.gene31932.t1
Gene Sequence

>MS.gene31932

ATGGTTTGTGGTTCTTGTCGCCGCCTGCTTTCGTATCCACCAGGTGCCAAACATGTCAAATGTTCATGCTGCCAGACAGTCAACATTGTATTAGAAGGTCAGTTCTCCTTTTTTCTCATGAACATGTCTATGCTGTGAAACTTTGCATTTTAAGTTCAATTTTATTTGAGAATCTCCAGCTGGTTCCTCGAAAATTAGCTTTTAGAAATTCGATTAAGAAGACTATATCGTACACGTTTTAGAGTATAACCCTGATACCATGTCAGGACTGGATGAAGATACGACCCCTGAAGGTAACACCCCAGTTATCAAAGCTCCAGCCAGCAGACCTCCGTCCTTTTCAAGGACCACTACCCAAATCTTCGTAGATCAACTACAACAGATGAACCATATCGGATCATTCACCATTCTTGGACCCAACAATCCCCGATTTATTGAACTGAATCGCAATGAAATTTTCAATGCTCTACGGGACCTTTACACCCTCCACTTTATGTCAAAGTGGGACATTTTGAAAAGAGTGGTTAAGTTCCATTGGAACTCCACCACTGCTGAGGAGGCTAAATACCTCTCTCAAGTTTATATCTCACATTGGTTCTTAGATGTTTATGCCTCAAACAGAGAGGCTTGCAGGAAGCTTCCCCTTGTTTGTACTTTGAACAAATTTGCAGTTTAGATAGGTATTCAATCAACAATTTATGATAATTACCTCACCCTATTGCTCAATGCTCTCAAGCCGACTCGGGTCACTGGAACGCCTGACAGCTCCATTCTCATTCCAATCATTTGCACCACCCCATTTTGGAACCACCTAGACGGTAACAACTATTTCAACATCGATGGATTTATCCTCAATACTGAACTCTTCAGCGCACTGATGCAGGTCATGACTGACCCAAACAGCAAATGGCTGATGAACACCTATTCCAATGACGTTCTAGGACGGCCATTTTGGCTACTTGACTGGCATAATGACCAAATATTTGCTTGGTTCCCAGAAGATGAGAACTATAACCAGGATGACATCAACCTGGCCCTAATTTTAGGAGTAGCCTGTACTCCACACCTAGCCCCAAGCGATGATCATATCCCACAGCAATATCCAAACAATGTCGTTCCCAACGCCGACACAATTTTCAAATATGAACGAAGAATTCCCGCTCAGTTCTACTCCAACTATGATCAACGAGTTGTTGGTCATCAAAAGTTGAAGATTACATGGGAGGAAATGCTAACCCATAATGAAATTGAAGCTGCAAGACAGAAGAATGAAGAAGAATACAATATCCAGCTTAAAGCTGAGTATACAAAGCTCGTCAACTCAAGACCTGCTGCAATGGATACAGCCGAAGGCTCAGGACAAGCCGTTGTCCCATTTCGCGCCACTGGCGAAATGATTGACCAAGAACTCATGGCTCATGCCAAGACGAAGATCAAGATGATAGACCCGCCATTGACAAAAGAGGATAGTTTTGTAGCTAACATGTATAAGATCATGGACTATAAGTACTACGCTCATGTAATTAGAAGGATTGACACTAACACCCGCACTGCAACTCATAAGCTGTTGATCTATAACGATTAGACTACCTTTTGTTTAACGCCTGATTGTAATCAAATTTCCTGTTATGTTAATGAAATTTTCTTTCAGTTTTATTTTATGCATCTGATTGAGTAAATGCTTTCATAACATATTCAACATTGTATTAGAAGGTCACTTATCTTTCTCTTTGCTTATTTTTCTCGTGAAAACGTATTTGCCATGAAACTTGCATTTTAAGTTCAGGTTTTTATACATCTGGTTGATTAATTACTTTCATAAACAATTACATGATTGGACGAACAGTCCTGTTATTGGTAGTTGTGTGCTTGCTGGTGCAAACAATCTTGTGTATTTAGTAAATTTTAATTTTAATAGTAACATTTCTAACAAAAAAGGAACTTCCTGGTCATTATAAAAGGGATACAGTCTAAGCACCAATGACCAATCACATTTGAATTTGATGATGCTATTATCATGCTCCAAATTTTCAATTAGACATTTTAGTATGGTAAACCTCTCCAAGAATGCATATACGCTATGTACTATTTGAAGTTTTTTCACTTGTCTAACTATATTCTATTATTTTGAAGTTCACTATACTTGTACAAAATATGACATGGCATTATTCTTTTGCATGGCAAGCTACTTATATCTTAACTCATTCCTTCGTCACAGCTGATCAGGTTGGACAAGTTAAGTGTGGGAGTTGTGCAGTATTGCTAATGTGCCCATACGGTGCTCCACAAGTTAGGTGTTCATCATGCCGGTTTGTGACAGAAATCGGGGTATGTAAACTGTTGACATTCACTAGTTCTCTTTATACATGTTTTACCTTCTAATTTTTTAAGTCAAAATAAACAATGTAACCAATTGCAAGTTGATTTATGTACATATTACATATTGTTCTAAGGTTTACTGACATATCATTAAGTTTGACACTTGATGTAGCGGATCTGATCTATACATATATATTATTACATTATATTCAACTATTTAAGTCTAGTTTTCTCTTTGTACGGTGTACTTCGTGCACTCGAAATAAAACAACTCATTTCAAACCTTTAAATTATCCAAGTGTAGGGGGATTGATGATGCTTTACTTTTGTGAATAATTGAAGACATTAAGTCCCACCCGCCTCTATTCATTCAATCTGAAGACAAACTATAGCTATTATTGATTTTATATTGTTGGCTTAAACAACTGATGTTTCCATTTAAACACTGATATCCTCATGATTTTTGTGCAGGCAGACAACAAGCGGCCTCCATGGTCTGTACAACAAAGTAAACCAACTCCTCCCAAATCTGGTTGTTAG
Protein sequence

>MS.gene31932.t1

MVCGSCRRLLSYPPGAKHVKCSCCQTVNIVLEADQVGQVKCGSCAVLLMCPYGAPQVRCSSCRFVTEIGADNKRPPWSVQQSKPTPPKSGC