Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31985.t1 | KEH43166.1 | 50 | 162 | 53 | 8 | 8 | 156 | 16 | 162 | 2.50E-23 | 119 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31985.t1 | A0A072VNE8 | 50.0 | 162 | 53 | 8 | 8 | 156 | 16 | 162 | 1.8e-23 | 119.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene31985.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31985.t1 | MTR_1g085590 | 53.540 | 226 | 58 | 11 | 7 | 203 | 15 | 222 | 3.23e-55 | 177 |
| MS.gene31985.t1 | MTR_1g063150 | 55.114 | 176 | 45 | 5 | 23 | 198 | 29 | 170 | 1.97e-51 | 164 |
| MS.gene31985.t1 | MTR_1g046810 | 37.349 | 249 | 76 | 12 | 22 | 204 | 82 | 316 | 4.24e-32 | 118 |
| MS.gene31985.t1 | MTR_1g063140 | 49.231 | 130 | 39 | 5 | 81 | 200 | 2 | 114 | 1.20e-21 | 87.8 |
| MS.gene31985.t1 | MTR_1g052185 | 31.220 | 205 | 79 | 9 | 54 | 204 | 82 | 278 | 4.83e-11 | 60.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 31 sgRNAs with CRISPR-Local
Find 35 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTGAGCGCCTGGCGTTTCT+TGG | 0.200333 | 4.3:-24583083 | None:intergenic |
| GAAAGAGAAAGAAGGGTATT+TGG | 0.272932 | 4.3:-24582825 | None:intergenic |
| AACCAAGACGAGACACTAAT+TGG | 0.312675 | 4.3:-24583395 | None:intergenic |
| TGAATCACAGCGGCGCTAAA+AGG | 0.334747 | 4.3:+24583212 | MS.gene31985:CDS |
| CTGAGCGCCTGGCGTTTCTT+GGG | 0.363120 | 4.3:-24583082 | None:intergenic |
| GGGTATTTGGCATTGGTTGT+TGG | 0.420713 | 4.3:-24582812 | None:intergenic |
| AAGCATTGAATGAACATAAG+AGG | 0.452841 | 4.3:+24582987 | MS.gene31985:CDS |
| CTGGAAAACAAAAGATTACA+TGG | 0.458402 | 4.3:+24582864 | MS.gene31985:CDS |
| GAAAGAAGGGTATTTGGCAT+TGG | 0.461253 | 4.3:-24582819 | None:intergenic |
| CATAATCACAATGATGTTGT+TGG | 0.465144 | 4.3:+24582911 | MS.gene31985:CDS |
| TATCAATGAAAGAGAAAGAA+GGG | 0.475697 | 4.3:-24582832 | None:intergenic |
| TTCATTGATACTAATCATGC+TGG | 0.476827 | 4.3:+24582845 | MS.gene31985:CDS |
| CTCTGTTGCATATATCTCGC+GGG | 0.495665 | 4.3:+24583158 | MS.gene31985:CDS |
| GCTGTGATTCACATCGATAT+TGG | 0.509973 | 4.3:-24583200 | None:intergenic |
| TGGTAGAAACTGATCATCAA+TGG | 0.511864 | 4.3:-24583059 | None:intergenic |
| CTCATGTGATGTTTGCAACA+AGG | 0.514548 | 4.3:+24582949 | MS.gene31985:CDS |
| AGCGCCTGGCGTTTCTTGGG+TGG | 0.517316 | 4.3:-24583079 | None:intergenic |
| GTATCAATGAAAGAGAAAGA+AGG | 0.525178 | 4.3:-24582833 | None:intergenic |
| TCATCTTCAAAATCATCACT+TGG | 0.541142 | 4.3:-24583370 | None:intergenic |
| AACTGATCATCAATGGAAGA+TGG | 0.551680 | 4.3:-24583052 | None:intergenic |
| AAGAGGTGTCACTCTCAAAA+CGG | 0.554314 | 4.3:+24583004 | MS.gene31985:CDS |
| TCGTTCAAATCCATATTGCT+TGG | 0.555538 | 4.3:-24583244 | None:intergenic |
| TTAACTTCAAAATCATCACT+TGG | 0.557014 | 4.3:-24583271 | None:intergenic |
| GTGATTCACATCGATATTGG+CGG | 0.581021 | 4.3:-24583197 | None:intergenic |
| ATGGAAGATGGTGCTGCAGT+TGG | 0.597422 | 4.3:-24583040 | None:intergenic |
| TCTACCACCCAAGAAACGCC+AGG | 0.621690 | 4.3:+24583075 | MS.gene31985:CDS |
| TCTGTTGCATATATCTCGCG+GGG | 0.628828 | 4.3:+24583159 | MS.gene31985:CDS |
| ACATCATTGTGATTATGAGT+AGG | 0.629847 | 4.3:-24582905 | None:intergenic |
| ACTCTGTTGCATATATCTCG+CGG | 0.638755 | 4.3:+24583157 | MS.gene31985:CDS |
| CTGTTGCATATATCTCGCGG+GGG | 0.734296 | 4.3:+24583160 | MS.gene31985:CDS |
| AATATCGATGTGAATCACAG+CGG | 0.785372 | 4.3:+24583202 | MS.gene31985:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TATCAATGAAAGAGAAAGAA+GGG | - | chr4.3:24582835-24582854 | None:intergenic | 25.0% |
| ! | TTAACTTCAAAATCATCACT+TGG | - | chr4.3:24583274-24583293 | None:intergenic | 25.0% |
| !!! | TGATGATTTTGAAGTTAATG+AGG | + | chr4.3:24583276-24583295 | MS.gene31985:CDS | 25.0% |
| ACATCATTGTGATTATGAGT+AGG | - | chr4.3:24582908-24582927 | None:intergenic | 30.0% | |
| CATAATCACAATGATGTTGT+TGG | + | chr4.3:24582911-24582930 | MS.gene31985:CDS | 30.0% | |
| CTGGAAAACAAAAGATTACA+TGG | + | chr4.3:24582864-24582883 | MS.gene31985:CDS | 30.0% | |
| GCAAAAAATTCCAAGCAATA+TGG | + | chr4.3:24583234-24583253 | MS.gene31985:CDS | 30.0% | |
| GCTCAGAAGAAAAAGATATA+TGG | + | chr4.3:24583099-24583118 | MS.gene31985:CDS | 30.0% | |
| GTATCAATGAAAGAGAAAGA+AGG | - | chr4.3:24582836-24582855 | None:intergenic | 30.0% | |
| TCATCTTCAAAATCATCACT+TGG | - | chr4.3:24583373-24583392 | None:intergenic | 30.0% | |
| TTCATTGATACTAATCATGC+TGG | + | chr4.3:24582845-24582864 | MS.gene31985:CDS | 30.0% | |
| !! | AAGCATTGAATGAACATAAG+AGG | + | chr4.3:24582987-24583006 | MS.gene31985:CDS | 30.0% |
| AACTGATCATCAATGGAAGA+TGG | - | chr4.3:24583055-24583074 | None:intergenic | 35.0% | |
| AATATCGATGTGAATCACAG+CGG | + | chr4.3:24583202-24583221 | MS.gene31985:CDS | 35.0% | |
| TCGTTCAAATCCATATTGCT+TGG | - | chr4.3:24583247-24583266 | None:intergenic | 35.0% | |
| TGGTAGAAACTGATCATCAA+TGG | - | chr4.3:24583062-24583081 | None:intergenic | 35.0% | |
| !! | GAAAGAGAAAGAAGGGTATT+TGG | - | chr4.3:24582828-24582847 | None:intergenic | 35.0% |
| ACTCTGTTGCATATATCTCG+CGG | + | chr4.3:24583157-24583176 | MS.gene31985:CDS | 40.0% | |
| CTCATGTGATGTTTGCAACA+AGG | + | chr4.3:24582949-24582968 | MS.gene31985:CDS | 40.0% | |
| GCTGTGATTCACATCGATAT+TGG | - | chr4.3:24583203-24583222 | None:intergenic | 40.0% | |
| GTGATTCACATCGATATTGG+CGG | - | chr4.3:24583200-24583219 | None:intergenic | 40.0% | |
| !! | AAGAGGTGTCACTCTCAAAA+CGG | + | chr4.3:24583004-24583023 | MS.gene31985:CDS | 40.0% |
| !! | GAAAGAAGGGTATTTGGCAT+TGG | - | chr4.3:24582822-24582841 | None:intergenic | 40.0% |
| CTCTGTTGCATATATCTCGC+GGG | + | chr4.3:24583158-24583177 | MS.gene31985:CDS | 45.0% | |
| TCTGTTGCATATATCTCGCG+GGG | + | chr4.3:24583159-24583178 | MS.gene31985:CDS | 45.0% | |
| ! | ATCTTTTTCTTCTGAGCGCC+TGG | - | chr4.3:24583096-24583115 | None:intergenic | 45.0% |
| !! | GGGTATTTGGCATTGGTTGT+TGG | - | chr4.3:24582815-24582834 | None:intergenic | 45.0% |
| ATATCTCGCGGGGGTTATGA+AGG | + | chr4.3:24583169-24583188 | MS.gene31985:CDS | 50.0% | |
| CTGTTGCATATATCTCGCGG+GGG | + | chr4.3:24583160-24583179 | MS.gene31985:CDS | 50.0% | |
| TGAATCACAGCGGCGCTAAA+AGG | + | chr4.3:24583212-24583231 | MS.gene31985:CDS | 50.0% | |
| !! | ATGGAAGATGGTGCTGCAGT+TGG | - | chr4.3:24583043-24583062 | None:intergenic | 50.0% |
| TCTACCACCCAAGAAACGCC+AGG | + | chr4.3:24583075-24583094 | MS.gene31985:CDS | 55.0% | |
| CTGAGCGCCTGGCGTTTCTT+GGG | - | chr4.3:24583085-24583104 | None:intergenic | 60.0% | |
| TCTGAGCGCCTGGCGTTTCT+TGG | - | chr4.3:24583086-24583105 | None:intergenic | 60.0% | |
| AGCGCCTGGCGTTTCTTGGG+TGG | - | chr4.3:24583082-24583101 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 24582788 | 24583402 | 24582788 | ID=MS.gene31985 |
| chr4.3 | mRNA | 24582788 | 24583402 | 24582788 | ID=MS.gene31985.t1;Parent=MS.gene31985 |
| chr4.3 | exon | 24582788 | 24583402 | 24582788 | ID=MS.gene31985.t1.exon1;Parent=MS.gene31985.t1 |
| chr4.3 | CDS | 24582788 | 24583402 | 24582788 | ID=cds.MS.gene31985.t1;Parent=MS.gene31985.t1 |
| Gene Sequence |
| Protein sequence |
