AlfalfaGEDB | MS.gene32041

M. sativa cultivar XinJiangDaYe / MS.gene32041

KEGG: -
GO: -
PFAM: PF04844.16

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene32041.t1	RHN80180.1	87.9	240	26	1	1	240	33	269	5.90E-88	334

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene32041.t1	O23341	64.1	64	23	0	176	239	109	172	3.4e-15	83.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene32041.t1	G7IDP8	87.9	240	26	1	1	240	1	237	4.3e-88	334.0

TFs/TRs:

Gene ID	Type	Classification
MS.gene32041.t1	TF	OFP

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene32041.t1	MTR_1g072110	95.417	240	8	2	1	240	1	237	2.57e-165	456
MS.gene32041.t1	MTR_7g095150	51.452	241	58	10	6	238	12	201	3.51e-60	189
MS.gene32041.t1	MTR_1g103640	47.101	138	50	3	102	237	63	179	1.17e-34	124
MS.gene32041.t1	MTR_2g102140	38.636	132	69	3	118	237	142	273	1.55e-17	80.5
MS.gene32041.t1	MTR_7g105780	40.157	127	52	5	111	237	97	199	1.99e-17	79.7
MS.gene32041.t1	MTR_4g012060	50.649	77	35	2	159	234	175	249	1.04e-15	75.1
MS.gene32041.t1	MTR_1g111640	51.429	70	29	2	166	235	135	199	8.33e-15	72.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene32041.t1	AT1G05420	37.220	223	87	7	49	239	19	220	1.01e-27	105
MS.gene32041.t1	AT1G05420	37.220	223	87	7	49	239	35	236	1.51e-27	105
MS.gene32041.t1	AT2G32100	37.423	163	72	5	95	239	88	238	7.51e-24	96.3
MS.gene32041.t1	AT4G14860	57.143	77	31	1	165	239	96	172	3.46e-22	90.5
MS.gene32041.t1	AT1G79960	45.946	74	40	0	164	237	187	260	3.39e-17	79.3
MS.gene32041.t1	AT5G04820	32.895	152	89	4	86	237	74	212	2.18e-15	73.6
MS.gene32041.t1	AT2G36050	33.600	125	55	2	113	237	77	173	8.72e-13	66.6
MS.gene32041.t1	AT3G52540	31.200	125	58	3	113	237	104	200	1.44e-11	63.2

Find 57 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCACTGATAGTTGTAGAAAT+TGG	0.240910	1.2:-55097059	None:intergenic
TTCCTCAGATTTCTTGTGTT+TGG	0.289544	1.2:-55096981	None:intergenic
AACACTCACAAGTTCATTCT+TGG	0.302219	1.2:+55097638	MS.gene32041:CDS
CTACTCACCTGATCCTTATA+TGG	0.303517	1.2:+55097499	MS.gene32041:CDS
CTTCTCACGCTCAATACTAT+TGG	0.339069	1.2:-55097420	None:intergenic
AAATCTGCTGGCTCAGGTTC+GGG	0.339475	1.2:-55097278	None:intergenic
TAAAGTGTGGTCTGAGGTTA+AGG	0.350644	1.2:-55097195	None:intergenic
ATGAACTTGTGAGTGTTCTT+TGG	0.354503	1.2:-55097632	None:intergenic
GATCTTCTAAAATCCATATA+AGG	0.364006	1.2:-55097512	None:intergenic
TGATGGATGTGAAATCAAAT+TGG	0.370791	1.2:+55097567	MS.gene32041:CDS
GAAATCTGCTGGCTCAGGTT+CGG	0.383556	1.2:-55097279	None:intergenic
AAGGCTGCTGCGAAATCTGC+TGG	0.394882	1.2:-55097290	None:intergenic
TGAACTTGTGAGTGTTCTTT+GGG	0.402029	1.2:-55097631	None:intergenic
TGTATATTCAATTAATGAGT+TGG	0.404911	1.2:-55097351	None:intergenic
GTTGTAGAAATTGGACTTGT+GGG	0.416852	1.2:-55097050	None:intergenic
CGCTTCTTCTTCTCCTCCCC+CGG	0.419956	1.2:+55097323	MS.gene32041:CDS
GAAACAGGAGAAAGAGAAAG+AGG	0.423520	1.2:+55097442	MS.gene32041:CDS
TATTGAGCGTGAGAAGAAAC+AGG	0.440175	1.2:+55097427	MS.gene32041:CDS
AATTCTTGATCATAATTGAC+GGG	0.444957	1.2:-55097151	None:intergenic
GCTGCGAAATCTGCTGGCTC+AGG	0.454177	1.2:-55097284	None:intergenic
AGAGATGGTGGAGGCGCGAC+CGG	0.455026	1.2:+55097541	MS.gene32041:CDS
CTAAAATCCATATAAGGATC+AGG	0.462583	1.2:-55097506	None:intergenic
AATTAATGAGTTGGAGCTAC+CGG	0.467305	1.2:-55097342	None:intergenic
ATACTATTGGACTTATCCAT+TGG	0.483511	1.2:-55097407	None:intergenic
GGAGGCGCGACCGGAGTTGA+TGG	0.488669	1.2:+55097550	MS.gene32041:CDS
AGATTGCATTCAGCATTCTT+CGG	0.492875	1.2:+55097379	MS.gene32041:CDS
TGTTCTTTGGGTTGAGAGCA+AGG	0.508816	1.2:-55097619	None:intergenic
GAAGAAGCGTTGAGAGGCGA+AGG	0.509416	1.2:-55097309	None:intergenic
ATTAATGAGTTGGAGCTACC+GGG	0.511814	1.2:-55097341	None:intergenic
CTATTGGACTTATCCATTGG+TGG	0.513854	1.2:-55097404	None:intergenic
TTCAACTTCGAGTTCAAAAG+AGG	0.515870	1.2:-55097255	None:intergenic
GCATTCTTCGGTACCACCAA+TGG	0.516823	1.2:+55097391	MS.gene32041:CDS
AAAAGAGGTTGATGTAGTAA+AGG	0.520478	1.2:-55097240	None:intergenic
AAATTCTTGATCATAATTGA+CGG	0.524121	1.2:-55097152	None:intergenic
GATGCAGATGTAGATGAAGA+AGG	0.533865	1.2:-55097125	None:intergenic
AGAGGTTGATGTAGTAAAGG+TGG	0.557401	1.2:-55097237	None:intergenic
ACCATGACTAAAATGACATG+AGG	0.563547	1.2:-55097685	None:intergenic
CATATAAGGATCAGGTGAGT+AGG	0.566346	1.2:-55097498	None:intergenic
GTGTGGTCTGAGGTTAAGGA+AGG	0.576057	1.2:-55097191	None:intergenic
TAGAAGATCGATGCAAGAGA+TGG	0.580424	1.2:+55097526	MS.gene32041:CDS
GATTTCACATCCATCAACTC+CGG	0.581756	1.2:-55097560	None:intergenic
AAGATCGATGCAAGAGATGG+TGG	0.583074	1.2:+55097529	MS.gene32041:CDS
AGTTGTAGAAATTGGACTTG+TGG	0.600672	1.2:-55097051	None:intergenic
ATCGATGCAAGAGATGGTGG+AGG	0.602628	1.2:+55097532	MS.gene32041:CDS
GGAGAAGAAGAAGCGTTGAG+AGG	0.620274	1.2:-55097315	None:intergenic
CATGAGGGTTACGAGAAGAT+CGG	0.623248	1.2:-55097669	None:intergenic
TTAATGAGTTGGAGCTACCG+GGG	0.639288	1.2:-55097340	None:intergenic
GTAGAAATTGGACTTGTGGG+TGG	0.648560	1.2:-55097047	None:intergenic
AGAGCATAAAGTGTGGTCTG+AGG	0.656840	1.2:-55097201	None:intergenic
TAAGGATCAGGTGAGTAGGT+CGG	0.659190	1.2:-55097494	None:intergenic
ATTCTTGATCATAATTGACG+GGG	0.668719	1.2:-55097150	None:intergenic
GTGAAGAAGAGCATAAAGTG+TGG	0.669604	1.2:-55097208	None:intergenic
CCATGACTAAAATGACATGA+GGG	0.681032	1.2:-55097684	None:intergenic
TGTGGTCTGAGGTTAAGGAA+GGG	0.682154	1.2:-55097190	None:intergenic
TTGATGATGATGTTGATGTG+AGG	0.685082	1.2:-55097090	None:intergenic
TAATGAGTTGGAGCTACCGG+GGG	0.686460	1.2:-55097339	None:intergenic
TGCCAAACACAAGAAATCTG+AGG	0.686722	1.2:+55096979	MS.gene32041:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAATTCTTGATCATAATTGA+CGG	-	chr1.2:55097155-55097174	None:intergenic	20.0%
!!	TGTATATTCAATTAATGAGT+TGG	-	chr1.2:55097354-55097373	None:intergenic	20.0%
!	AATTCTTGATCATAATTGAC+GGG	-	chr1.2:55097154-55097173	None:intergenic	25.0%
!	AATTTGAAAAACATAGGTTG+AGG	-	chr1.2:55097007-55097026	None:intergenic	25.0%
!	GAGTTGAATTTGAAAAACAT+AGG	-	chr1.2:55097013-55097032	None:intergenic	25.0%
!!!	GATCTTCTAAAATCCATATA+AGG	-	chr1.2:55097515-55097534	None:intergenic	25.0%
	ATACTATTGGACTTATCCAT+TGG	-	chr1.2:55097410-55097429	None:intergenic	30.0%
	ATTCTTGATCATAATTGACG+GGG	-	chr1.2:55097153-55097172	None:intergenic	30.0%
	CTAAAATCCATATAAGGATC+AGG	-	chr1.2:55097509-55097528	None:intergenic	30.0%
	TCACTGATAGTTGTAGAAAT+TGG	-	chr1.2:55097062-55097081	None:intergenic	30.0%
!	TGATGGATGTGAAATCAAAT+TGG	+	chr1.2:55097567-55097586	MS.gene32041:CDS	30.0%
!!	AAAAGAGGTTGATGTAGTAA+AGG	-	chr1.2:55097243-55097262	None:intergenic	30.0%
!!!	AAAGAGGAAGTTTTGTTCAA+CGG	+	chr1.2:55097458-55097477	MS.gene32041:CDS	30.0%
	AACACTCACAAGTTCATTCT+TGG	+	chr1.2:55097638-55097657	MS.gene32041:CDS	35.0%
	AGATTGCATTCAGCATTCTT+CGG	+	chr1.2:55097379-55097398	MS.gene32041:CDS	35.0%
	AGTTGTAGAAATTGGACTTG+TGG	-	chr1.2:55097054-55097073	None:intergenic	35.0%
	ATGAACTTGTGAGTGTTCTT+TGG	-	chr1.2:55097635-55097654	None:intergenic	35.0%
	GTTGTAGAAATTGGACTTGT+GGG	-	chr1.2:55097053-55097072	None:intergenic	35.0%
	TGAACTTGTGAGTGTTCTTT+GGG	-	chr1.2:55097634-55097653	None:intergenic	35.0%
	TTCCTCAGATTTCTTGTGTT+TGG	-	chr1.2:55096984-55097003	None:intergenic	35.0%
!	AATTAATGAGTTGGAGCTAC+CGG	-	chr1.2:55097345-55097364	None:intergenic	35.0%
!	TTCAACTTCGAGTTCAAAAG+AGG	-	chr1.2:55097258-55097277	None:intergenic	35.0%
!!	TTGATGATGATGTTGATGTG+AGG	-	chr1.2:55097093-55097112	None:intergenic	35.0%
	CATATAAGGATCAGGTGAGT+AGG	-	chr1.2:55097501-55097520	None:intergenic	40.0%
	CTACTCACCTGATCCTTATA+TGG	+	chr1.2:55097499-55097518	MS.gene32041:CDS	40.0%
	CTATTGGACTTATCCATTGG+TGG	-	chr1.2:55097407-55097426	None:intergenic	40.0%
	CTTCTCACGCTCAATACTAT+TGG	-	chr1.2:55097423-55097442	None:intergenic	40.0%
	GAAACAGGAGAAAGAGAAAG+AGG	+	chr1.2:55097442-55097461	MS.gene32041:CDS	40.0%
	GATGCAGATGTAGATGAAGA+AGG	-	chr1.2:55097128-55097147	None:intergenic	40.0%
	GATTTCACATCCATCAACTC+CGG	-	chr1.2:55097563-55097582	None:intergenic	40.0%
	TAGAAGATCGATGCAAGAGA+TGG	+	chr1.2:55097526-55097545	MS.gene32041:CDS	40.0%
	TATTGAGCGTGAGAAGAAAC+AGG	+	chr1.2:55097427-55097446	MS.gene32041:CDS	40.0%
	TGCCAAACACAAGAAATCTG+AGG	+	chr1.2:55096979-55096998	MS.gene32041:CDS	40.0%
!	ATTAATGAGTTGGAGCTACC+GGG	-	chr1.2:55097344-55097363	None:intergenic	40.0%
!	GTGAAGAAGAGCATAAAGTG+TGG	-	chr1.2:55097211-55097230	None:intergenic	40.0%
!	TAAAGTGTGGTCTGAGGTTA+AGG	-	chr1.2:55097198-55097217	None:intergenic	40.0%
!!	AGAGGTTGATGTAGTAAAGG+TGG	-	chr1.2:55097240-55097259	None:intergenic	40.0%
!!!	AGTTTTGTTCAACGGAAGTG+TGG	+	chr1.2:55097466-55097485	MS.gene32041:CDS	40.0%
	AAGATCGATGCAAGAGATGG+TGG	+	chr1.2:55097529-55097548	MS.gene32041:CDS	45.0%
	AGAGCATAAAGTGTGGTCTG+AGG	-	chr1.2:55097204-55097223	None:intergenic	45.0%
	CATGAGGGTTACGAGAAGAT+CGG	-	chr1.2:55097672-55097691	None:intergenic	45.0%
	GTAGAAATTGGACTTGTGGG+TGG	-	chr1.2:55097050-55097069	None:intergenic	45.0%
	TAAGGATCAGGTGAGTAGGT+CGG	-	chr1.2:55097497-55097516	None:intergenic	45.0%
!	TGTGGTCTGAGGTTAAGGAA+GGG	-	chr1.2:55097193-55097212	None:intergenic	45.0%
!	TTAATGAGTTGGAGCTACCG+GGG	-	chr1.2:55097343-55097362	None:intergenic	45.0%
!!	TGTTCTTTGGGTTGAGAGCA+AGG	-	chr1.2:55097622-55097641	None:intergenic	45.0%
	GCATTCTTCGGTACCACCAA+TGG	+	chr1.2:55097391-55097410	MS.gene32041:CDS	50.0%
!	ATCGATGCAAGAGATGGTGG+AGG	+	chr1.2:55097532-55097551	MS.gene32041:CDS	50.0%
!	GTGTGGTCTGAGGTTAAGGA+AGG	-	chr1.2:55097194-55097213	None:intergenic	50.0%
!	TAATGAGTTGGAGCTACCGG+GGG	-	chr1.2:55097342-55097361	None:intergenic	50.0%
!!	AAATCTGCTGGCTCAGGTTC+GGG	-	chr1.2:55097281-55097300	None:intergenic	50.0%
!!	GAAATCTGCTGGCTCAGGTT+CGG	-	chr1.2:55097282-55097301	None:intergenic	50.0%
!!	GGAGAAGAAGAAGCGTTGAG+AGG	-	chr1.2:55097318-55097337	None:intergenic	50.0%
	AAGGCTGCTGCGAAATCTGC+TGG	-	chr1.2:55097293-55097312	None:intergenic	55.0%
!!	GAAGAAGCGTTGAGAGGCGA+AGG	-	chr1.2:55097312-55097331	None:intergenic	55.0%
	CGCTTCTTCTTCTCCTCCCC+CGG	+	chr1.2:55097323-55097342	MS.gene32041:CDS	60.0%
!!	GCTGCGAAATCTGCTGGCTC+AGG	-	chr1.2:55097287-55097306	None:intergenic	60.0%
!	TGAGTTGGAGCTACCGGGGG+AGG	-	chr1.2:55097339-55097358	None:intergenic	65.0%
!!	AGAGATGGTGGAGGCGCGAC+CGG	+	chr1.2:55097541-55097560	MS.gene32041:CDS	65.0%
!	GGAGGCGCGACCGGAGTTGA+TGG	+	chr1.2:55097550-55097569	MS.gene32041:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	55096978	55097700	55096978	ID=MS.gene32041
chr1.2	mRNA	55096978	55097700	55096978	ID=MS.gene32041.t1;Parent=MS.gene32041
chr1.2	exon	55096978	55097700	55096978	ID=MS.gene32041.t1.exon1;Parent=MS.gene32041.t1
chr1.2	CDS	55096978	55097700	55096978	ID=cds.MS.gene32041.t1;Parent=MS.gene32041.t1

Gene Sequence

>MS.gene32041

ATGCCAAACACAAGAAATCTGAGGAACCTCAACCTATGTTTTTCAAATTCAACTCTTGAATCAAAATCTCCACCCACAAGTCCAATTTCTACAACTATCAGTGAAGCAGCTTCCTCACATCAACATCATCATCAAATTCATCACAAACCTTCTTCATCTACATCTGCATCTTCCCCGTCAATTATGATCAAGAATTTCAACTCTCTCTATGACCCTTCCTTAACCTCAGACCACACTTTATGCTCTTCTTCACTCTCTACCACCTTTACTACATCAACCTCTTTTGAACTCGAAGTTGAACCCGAACCTGAGCCAGCAGATTTCGCAGCAGCCTTCGCCTCTCAACGCTTCTTCTTCTCCTCCCCCGGTAGCTCCAACTCATTAATTGAATATACAAACACAGATTGCATTCAGCATTCTTCGGTACCACCAATGGATAAGTCCAATAGTATTGAGCGTGAGAAGAAACAGGAGAAAGAGAAAGAGGAAGTTTTGTTCAACGGAAGTGTGGCAGTACCGACCTACTCACCTGATCCTTATATGGATTTTAGAAGATCGATGCAAGAGATGGTGGAGGCGCGACCGGAGTTGATGGATGTGAAATCAAATTGGAACATCTTGCATGAGTTGCTTCTTTGTTACCTTGCTCTCAACCCAAAGAACACTCACAAGTTCATTCTTGGTGCTTTTGCCGATCTTCTCGTAACCCTCATGTCATTTTAG

Protein sequence

>MS.gene32041.t1

MPNTRNLRNLNLCFSNSTLESKSPPTSPISTTISEAASSHQHHHQIHHKPSSSTSASSPSIMIKNFNSLYDPSLTSDHTLCSSSLSTTFTTSTSFELEVEPEPEPADFAAAFASQRFFFSSPGSSNSLIEYTNTDCIQHSSVPPMDKSNSIEREKKQEKEKEEVLFNGSVAVPTYSPDPYMDFRRSMQEMVEARPELMDVKSNWNILHELLLCYLALNPKNTHKFILGAFADLLVTLMSF