AlfalfaGEDB | MS.gene32327

M. sativa cultivar XinJiangDaYe / MS.gene32327

KEGG: K08065
GO: GO:0003674 GO:0005488 GO:0005515 GO:0046982 GO:0046983
PFAM: PF00808.26

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene32327.t1	AFK49669.1	85.1	87	13	0	41	127	1	87	4.60E-30	141

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene32327.t1	O23310	34.3	108	70	1	24	131	4	110	3.1e-11	69.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene32327.t1	I3TAX7	85.1	87	13	0	41	127	1	87	3.3e-30	141.0

TFs/TRs:

Gene ID	Type	Classification
MS.gene32327.t1	TF	NF-YB

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene32327.t1	MTR_1g029100	82.222	90	16	0	41	130	1	90	1.40e-48	151
MS.gene32327.t1	MTR_1g029070	38.462	104	64	0	27	130	37	140	7.14e-20	81.3
MS.gene32327.t1	MTR_4g133952	37.647	85	53	0	47	131	63	147	4.06e-19	80.9
MS.gene32327.t1	MTR_1g039040	39.785	93	55	1	47	139	11	102	6.03e-19	79.3
MS.gene32327.t1	MTR_1g072790	36.634	101	54	1	47	137	34	134	1.48e-18	77.8
MS.gene32327.t1	MTR_3g058980	36.364	99	62	1	47	145	32	129	3.86e-18	77.0
MS.gene32327.t1	MTR_7g061270	34.579	107	67	1	47	150	31	137	8.12e-18	75.9
MS.gene32327.t1	MTR_1g088860	37.647	85	53	0	47	131	41	125	8.50e-18	76.3
MS.gene32327.t1	MTR_5g095740	38.824	85	52	0	47	131	28	112	1.12e-17	75.9
MS.gene32327.t1	MTR_7g100650	34.579	107	67	1	47	150	31	137	1.18e-17	75.5
MS.gene32327.t1	MTR_8g091720	36.634	101	63	1	47	147	33	132	1.29e-17	75.9
MS.gene32327.t1	MTR_2g026710	38.202	89	54	1	47	135	22	109	2.41e-17	74.7
MS.gene32327.t1	MTR_4g133938	37.079	89	56	0	43	131	20	108	3.36e-17	73.6
MS.gene32327.t1	MTR_0392s0020	30.909	110	66	1	22	131	8	107	5.41e-17	72.8
MS.gene32327.t1	MTR_4g119500	34.118	85	56	0	47	131	27	111	1.88e-16	71.2
MS.gene32327.t1	MTR_8g093920	38.095	84	52	0	47	130	45	128	3.01e-16	72.8
MS.gene32327.t1	MTR_1g083070	31.765	85	58	0	47	131	9	93	1.14e-15	69.3
MS.gene32327.t1	MTR_1g028480	38.372	86	39	1	78	149	25	110	6.39e-15	66.6
MS.gene32327.t1	MTR_5g095900	31.325	83	57	0	48	130	22	104	2.29e-11	59.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene32327.t1	AT2G38880	36.508	126	69	3	32	146	1	126	1.99e-20	82.0
MS.gene32327.t1	AT2G38880	35.938	128	69	3	32	146	1	128	3.58e-20	81.3
MS.gene32327.t1	AT2G38880	35.938	128	69	3	32	146	1	128	4.17e-20	81.3
MS.gene32327.t1	AT2G38880	35.938	128	69	3	32	146	1	128	4.17e-20	81.3
MS.gene32327.t1	AT2G38880	35.938	128	69	3	32	146	1	128	4.17e-20	81.3
MS.gene32327.t1	AT3G53340	38.679	106	62	1	47	149	34	139	1.29e-19	80.9
MS.gene32327.t1	AT3G53340	38.679	106	62	1	47	149	34	139	1.29e-19	80.9
MS.gene32327.t1	AT2G37060	37.736	106	63	1	47	149	35	140	3.45e-19	79.7
MS.gene32327.t1	AT2G37060	37.736	106	63	1	47	149	35	140	3.45e-19	79.7
MS.gene32327.t1	AT2G37060	37.736	106	63	1	47	149	35	140	3.45e-19	79.7
MS.gene32327.t1	AT2G38880	37.037	108	58	2	34	131	3	110	8.13e-19	77.0
MS.gene32327.t1	AT2G38880	37.037	108	58	2	34	131	3	110	2.46e-18	76.6
MS.gene32327.t1	AT2G38880	37.037	108	58	2	34	131	3	110	2.46e-18	76.6
MS.gene32327.t1	AT2G38880	37.037	108	58	2	34	131	3	110	2.46e-18	76.6
MS.gene32327.t1	AT2G38880	36.364	110	60	2	32	131	1	110	2.89e-18	77.0
MS.gene32327.t1	AT2G38880	36.364	110	60	2	32	131	1	110	2.89e-18	77.0
MS.gene32327.t1	AT4G14540	38.824	85	52	0	47	131	26	110	5.53e-18	76.3
MS.gene32327.t1	AT1G21970	33.645	107	71	0	25	131	42	148	6.19e-18	77.8
MS.gene32327.t1	AT5G47640	40.000	85	51	0	47	131	32	116	8.06e-18	76.3
MS.gene32327.t1	AT5G47670	34.737	95	62	0	47	141	34	128	1.40e-17	76.3
MS.gene32327.t1	AT5G47670	34.737	95	62	0	47	141	63	157	2.84e-17	75.9
MS.gene32327.t1	AT5G47670	34.737	95	62	0	47	141	63	157	2.84e-17	75.9
MS.gene32327.t1	AT3G53340	40.000	85	51	0	47	131	34	118	4.55e-17	72.8
MS.gene32327.t1	AT2G47810	34.146	123	72	3	32	145	38	160	5.24e-17	73.6
MS.gene32327.t1	AT3G53340	38.947	95	57	1	47	141	34	127	1.59e-16	72.0
MS.gene32327.t1	AT3G53340	38.947	95	57	1	47	141	34	127	1.59e-16	72.0
MS.gene32327.t1	AT2G13570	38.095	84	52	0	47	130	41	124	3.89e-15	69.7
MS.gene32327.t1	AT1G09030	28.235	85	61	0	47	131	8	92	1.43e-12	61.6

Find 32 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGCACTTCCTCGCCCATTGC+TGG	0.309871	1.4:-14201253	None:intergenic
TTTCTGCTGATGATTTACTA+TGG	0.322953	1.4:+14201440	MS.gene32327:CDS
ATTTACTATGGACGATGAAT+AGG	0.363355	1.4:+14201452	MS.gene32327:CDS
GAGGAACATGTTCGGTCTCT+TGG	0.363705	1.4:+14201484	MS.gene32327:CDS
GATGCTCTACATTGGCTTGT+TGG	0.407680	1.4:-14201408	None:intergenic
GATGAATAGGTTGGGCTTTG+AGG	0.431149	1.4:+14201465	MS.gene32327:CDS
AGGAACATGTTCGGTCTCTT+GGG	0.445239	1.4:+14201485	MS.gene32327:CDS
CTATGGACGATGAATAGGTT+GGG	0.452990	1.4:+14201457	MS.gene32327:CDS
ACCAACAACAACACTGGCAT+TGG	0.458777	1.4:+14201208	MS.gene32327:CDS
GCCAGTGTTGTTGTTGGTGG+TGG	0.460032	1.4:-14201203	None:intergenic
CAACACTGGCATTGGAATTG+AGG	0.464074	1.4:+14201216	MS.gene32327:CDS
TCAACGTTGGTCATCATCAT+TGG	0.472149	1.4:-14201569	None:intergenic
CGCAAGAACATGGATGGGTC+AGG	0.479316	1.4:+14201526	MS.gene32327:CDS
TCCAATGCCAGTGTTGTTGT+TGG	0.479723	1.4:-14201209	None:intergenic
CAACATTCAACTCCAGCAAT+GGG	0.494824	1.4:+14201241	MS.gene32327:CDS
AATGCCAGTGTTGTTGTTGG+TGG	0.494945	1.4:-14201206	None:intergenic
GACTCTTGTCACATGACTGA+TGG	0.498145	1.4:-14201281	None:intergenic
AACTATCGCAAGAACATGGA+TGG	0.501574	1.4:+14201520	MS.gene32327:CDS
TGGGCTTTGAGGAACATGTT+CGG	0.504221	1.4:+14201476	MS.gene32327:CDS
TCATCAGTGATTGTTGTGTC+TGG	0.515266	1.4:-14201322	None:intergenic
ACTATGGACGATGAATAGGT+TGG	0.532041	1.4:+14201456	MS.gene32327:CDS
CCATTGCTGGAGTTGAATGT+TGG	0.538932	1.4:-14201240	None:intergenic
CCAACATTCAACTCCAGCAA+TGG	0.540089	1.4:+14201240	MS.gene32327:CDS
AACAATCACTGATGATGCTA+AGG	0.542129	1.4:+14201330	MS.gene32327:CDS
ATGGAATTTCATATCAACGT+TGG	0.550067	1.4:-14201582	None:intergenic
ATTCTCTTGATGCTCTACAT+TGG	0.555958	1.4:-14201416	None:intergenic
TTCAACTCCAGCAATGGGCG+AGG	0.564498	1.4:+14201246	MS.gene32327:CDS
TGTGTCTAAATTTATGAACA+AGG	0.573477	1.4:+14201369	MS.gene32327:CDS
ATGGATGGGTCAGGTGCTAG+TGG	0.576300	1.4:+14201535	MS.gene32327:CDS
ACCACCACCAACAACAACAC+TGG	0.591133	1.4:+14201202	MS.gene32327:CDS
TATGAACAAGGTCACAAGTG+AGG	0.614031	1.4:+14201381	MS.gene32327:CDS
ACTATCGCAAGAACATGGAT+GGG	0.635414	1.4:+14201521	MS.gene32327:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TGTGTCTAAATTTATGAACA+AGG	+	chr1.4:14201369-14201388	MS.gene32327:CDS	25.0%
!!!	AGCTAAGATGTTTTTAATAG+AGG	-	chr1.4:14201173-14201192	None:intergenic	25.0%
	ATTTACTATGGACGATGAAT+AGG	+	chr1.4:14201452-14201471	MS.gene32327:CDS	30.0%
	TTTCTGCTGATGATTTACTA+TGG	+	chr1.4:14201440-14201459	MS.gene32327:CDS	30.0%
	ATTCTCTTGATGCTCTACAT+TGG	-	chr1.4:14201419-14201438	None:intergenic	35.0%
	CAAAAACTATCGCAAGAACA+TGG	+	chr1.4:14201516-14201535	MS.gene32327:CDS	35.0%
!	AACAATCACTGATGATGCTA+AGG	+	chr1.4:14201330-14201349	MS.gene32327:CDS	35.0%
!	TGTTCTTGCGATAGTTTTTG+AGG	-	chr1.4:14201516-14201535	None:intergenic	35.0%
	AACTATCGCAAGAACATGGA+TGG	+	chr1.4:14201520-14201539	MS.gene32327:CDS	40.0%
	ACTATCGCAAGAACATGGAT+GGG	+	chr1.4:14201521-14201540	MS.gene32327:CDS	40.0%
	ACTATGGACGATGAATAGGT+TGG	+	chr1.4:14201456-14201475	MS.gene32327:CDS	40.0%
	CAACATTCAACTCCAGCAAT+GGG	+	chr1.4:14201241-14201260	MS.gene32327:CDS	40.0%
	CTATGGACGATGAATAGGTT+GGG	+	chr1.4:14201457-14201476	MS.gene32327:CDS	40.0%
	TATGAACAAGGTCACAAGTG+AGG	+	chr1.4:14201381-14201400	MS.gene32327:CDS	40.0%
	TCAACGTTGGTCATCATCAT+TGG	-	chr1.4:14201572-14201591	None:intergenic	40.0%
	TCATCAGTGATTGTTGTGTC+TGG	-	chr1.4:14201325-14201344	None:intergenic	40.0%
	ACCAACAACAACACTGGCAT+TGG	+	chr1.4:14201208-14201227	MS.gene32327:CDS	45.0%
	AGGAACATGTTCGGTCTCTT+GGG	+	chr1.4:14201485-14201504	MS.gene32327:CDS	45.0%
	CCAACATTCAACTCCAGCAA+TGG	+	chr1.4:14201240-14201259	MS.gene32327:CDS	45.0%
	CCATTGCTGGAGTTGAATGT+TGG	-	chr1.4:14201243-14201262	None:intergenic	45.0%
	GACTCTTGTCACATGACTGA+TGG	-	chr1.4:14201284-14201303	None:intergenic	45.0%
!	AATGCCAGTGTTGTTGTTGG+TGG	-	chr1.4:14201209-14201228	None:intergenic	45.0%
!	CAACACTGGCATTGGAATTG+AGG	+	chr1.4:14201216-14201235	MS.gene32327:CDS	45.0%
!	GATGAATAGGTTGGGCTTTG+AGG	+	chr1.4:14201465-14201484	MS.gene32327:CDS	45.0%
!	TCCAATGCCAGTGTTGTTGT+TGG	-	chr1.4:14201212-14201231	None:intergenic	45.0%
!!	GATGCTCTACATTGGCTTGT+TGG	-	chr1.4:14201411-14201430	None:intergenic	45.0%
!!	TGGGCTTTGAGGAACATGTT+CGG	+	chr1.4:14201476-14201495	MS.gene32327:CDS	45.0%
	ACCACCACCAACAACAACAC+TGG	+	chr1.4:14201202-14201221	MS.gene32327:CDS	50.0%
	GAGGAACATGTTCGGTCTCT+TGG	+	chr1.4:14201484-14201503	MS.gene32327:CDS	50.0%
	ATGGATGGGTCAGGTGCTAG+TGG	+	chr1.4:14201535-14201554	MS.gene32327:CDS	55.0%
	CGCAAGAACATGGATGGGTC+AGG	+	chr1.4:14201526-14201545	MS.gene32327:CDS	55.0%
	TTCAACTCCAGCAATGGGCG+AGG	+	chr1.4:14201246-14201265	MS.gene32327:CDS	55.0%
!	GCCAGTGTTGTTGTTGGTGG+TGG	-	chr1.4:14201206-14201225	None:intergenic	55.0%
	CGCACTTCCTCGCCCATTGC+TGG	-	chr1.4:14201256-14201275	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.4	gene	14201139	14201591	14201139	ID=MS.gene32327
chr1.4	mRNA	14201139	14201591	14201139	ID=MS.gene32327.t1;Parent=MS.gene32327
chr1.4	exon	14201139	14201591	14201139	ID=MS.gene32327.t1.exon1;Parent=MS.gene32327.t1
chr1.4	CDS	14201139	14201591	14201139	ID=cds.MS.gene32327.t1;Parent=MS.gene32327.t1

Gene Sequence

>MS.gene32327

ATGATTGATTTTTTTTTCTTTTTTTCTTTTACCTCTATTAAAAACATCTTAGCTGAAAGAAACACCACCACCAACAACAACACTGGCATTGGAATTGAGGACCAACATTCAACTCCAGCAATGGGCGAGGAAGTGCGTATGTCCATCAGTCATGTGACAAGAGTCATTCAGAGTGTTCTTTCACCAGACACAACAATCACTGATGATGCTAAGGAGTTAATGCAATTGTTTGTGTCTAAATTTATGAACAAGGTCACAAGTGAGGCTTTCCAACAAGCCAATGTAGAGCATCAAGAGAATGTTTCTGCTGATGATTTACTATGGACGATGAATAGGTTGGGCTTTGAGGAACATGTTCGGTCTCTTGGGAAATACCTCAAAAACTATCGCAAGAACATGGATGGGTCAGGTGCTAGTGGCTCAAACAATGCCAATGATGATGACCAACGTTGA

Protein sequence

>MS.gene32327.t1

MIDFFFFFSFTSIKNILAERNTTTNNNTGIGIEDQHSTPAMGEEVRMSISHVTRVIQSVLSPDTTITDDAKELMQLFVSKFMNKVTSEAFQQANVEHQENVSADDLLWTMNRLGFEEHVRSLGKYLKNYRKNMDGSGASGSNNANDDDQR