AlfalfaGEDB | MS.gene32327

M. sativa cultivar XinJiangDaYe / MS.gene32327

KEGG: K08065
GO: GO:0003674 GO:0005488 GO:0005515 GO:0046982 GO:0046983
PFAM: PF00808.26

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene32327.t1	AFK49669.1	85.1	87	13	0	41	127	1	87	4.60E-30	141

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene32327.t1	O23310	34.3	108	70	1	24	131	4	110	3.1e-11	69.7

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene32327.t1	I3TAX7	85.1	87	13	0	41	127	1	87	3.3e-30	141.0

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene32327.t1	TF	NF-YB

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene32327.t1	MTR_1g029100	82.222	90	16	0	41	130	1	90	1.40e-48	151
MS.gene32327.t1	MTR_1g029070	38.462	104	64	0	27	130	37	140	7.14e-20	81.3
MS.gene32327.t1	MTR_4g133952	37.647	85	53	0	47	131	63	147	4.06e-19	80.9
MS.gene32327.t1	MTR_1g039040	39.785	93	55	1	47	139	11	102	6.03e-19	79.3
MS.gene32327.t1	MTR_1g072790	36.634	101	54	1	47	137	34	134	1.48e-18	77.8
MS.gene32327.t1	MTR_3g058980	36.364	99	62	1	47	145	32	129	3.86e-18	77.0
MS.gene32327.t1	MTR_7g061270	34.579	107	67	1	47	150	31	137	8.12e-18	75.9
MS.gene32327.t1	MTR_1g088860	37.647	85	53	0	47	131	41	125	8.50e-18	76.3
MS.gene32327.t1	MTR_5g095740	38.824	85	52	0	47	131	28	112	1.12e-17	75.9
MS.gene32327.t1	MTR_7g100650	34.579	107	67	1	47	150	31	137	1.18e-17	75.5

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene32327.t1	AT2G38880	36.508	126	69	3	32	146	1	126	1.99e-20	82.0
MS.gene32327.t1	AT2G38880	35.938	128	69	3	32	146	1	128	3.58e-20	81.3
MS.gene32327.t1	AT2G38880	35.938	128	69	3	32	146	1	128	4.17e-20	81.3
MS.gene32327.t1	AT2G38880	35.938	128	69	3	32	146	1	128	4.17e-20	81.3
MS.gene32327.t1	AT2G38880	35.938	128	69	3	32	146	1	128	4.17e-20	81.3
MS.gene32327.t1	AT3G53340	38.679	106	62	1	47	149	34	139	1.29e-19	80.9
MS.gene32327.t1	AT3G53340	38.679	106	62	1	47	149	34	139	1.29e-19	80.9
MS.gene32327.t1	AT2G37060	37.736	106	63	1	47	149	35	140	3.45e-19	79.7
MS.gene32327.t1	AT2G37060	37.736	106	63	1	47	149	35	140	3.45e-19	79.7
MS.gene32327.t1	AT2G37060	37.736	106	63	1	47	149	35	140	3.45e-19	79.7

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Find 32 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACAATCACTGATGATGCTA+AGG	0.542129	1.4:+14201330	MS.gene32327:CDS
AACTATCGCAAGAACATGGA+TGG	0.501574	1.4:+14201520	MS.gene32327:CDS
AATGCCAGTGTTGTTGTTGG+TGG	0.494945	1.4:-14201206	None:intergenic
ACCAACAACAACACTGGCAT+TGG	0.458777	1.4:+14201208	MS.gene32327:CDS
ACCACCACCAACAACAACAC+TGG	0.591133	1.4:+14201202	MS.gene32327:CDS
ACTATCGCAAGAACATGGAT+GGG	0.635414	1.4:+14201521	MS.gene32327:CDS
ACTATGGACGATGAATAGGT+TGG	0.532041	1.4:+14201456	MS.gene32327:CDS
AGGAACATGTTCGGTCTCTT+GGG	0.445239	1.4:+14201485	MS.gene32327:CDS
ATGGAATTTCATATCAACGT+TGG	0.550067	1.4:-14201582	None:intergenic
ATGGATGGGTCAGGTGCTAG+TGG	0.576300	1.4:+14201535	MS.gene32327:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATTTACTATGGACGATGAAT+AGG	+	chr1.4:14201452-14201471	MS.gene32327:CDS	30.0%
TTTCTGCTGATGATTTACTA+TGG	+	chr1.4:14201440-14201459	MS.gene32327:CDS	30.0%
ATTCTCTTGATGCTCTACAT+TGG	-	chr1.4:14201419-14201438	None:intergenic	35.0%
CAAAAACTATCGCAAGAACA+TGG	+	chr1.4:14201516-14201535	MS.gene32327:CDS	35.0%
AACTATCGCAAGAACATGGA+TGG	+	chr1.4:14201520-14201539	MS.gene32327:CDS	40.0%
ACTATCGCAAGAACATGGAT+GGG	+	chr1.4:14201521-14201540	MS.gene32327:CDS	40.0%
ACTATGGACGATGAATAGGT+TGG	+	chr1.4:14201456-14201475	MS.gene32327:CDS	40.0%
CAACATTCAACTCCAGCAAT+GGG	+	chr1.4:14201241-14201260	MS.gene32327:CDS	40.0%
CTATGGACGATGAATAGGTT+GGG	+	chr1.4:14201457-14201476	MS.gene32327:CDS	40.0%
TATGAACAAGGTCACAAGTG+AGG	+	chr1.4:14201381-14201400	MS.gene32327:CDS	40.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1.4	gene	14201139	14201591	14201139	ID=MS.gene32327
chr1.4	mRNA	14201139	14201591	14201139	ID=MS.gene32327.t1;Parent=MS.gene32327
chr1.4	exon	14201139	14201591	14201139	ID=MS.gene32327.t1.exon1;Parent=MS.gene32327.t1
chr1.4	CDS	14201139	14201591	14201139	ID=cds.MS.gene32327.t1;Parent=MS.gene32327.t1

Gene Sequence

>MS.gene32327

ATGATTGATTTTTTTTTCTTTTTTTCTTTTACCTCTATTAAAAACATCTTAGCTGAAAGAAACACCACCACCAACAACAACACTGGCATTGGAATTGAGGACCAACATTCAACTCCAGCAATGGGCGAGGAAGTGCGTATGTCCATCAGTCATGTGACAAGAGTCATTCAGAGTGTTCTTTCACCAGACACAACAATCACTGATGATGCTAAGGAGTTAATGCAATTGTTTGTGTCTAAATTTATGAACAAGGTCACAAGTGAGGCTTTCCAACAAGCCAATGTAGAGCATCAAGAGAATGTTTCTGCTGATGATTTACTATGGACGATGAATAGGTTGGGCTTTGAGGAACATGTTCGGTCTCTTGGGAAATACCTCAAAAACTATCGCAAGAACATGGATGGGTCAGGTGCTAGTGGCTCAAACAATGCCAATGATGATGACCAACGTTGA

Protein sequence

>MS.gene32327.t1

MIDFFFFFSFTSIKNILAERNTTTNNNTGIGIEDQHSTPAMGEEVRMSISHVTRVIQSVLSPDTTITDDAKELMQLFVSKFMNKVTSEAFQQANVEHQENVSADDLLWTMNRLGFEEHVRSLGKYLKNYRKNMDGSGASGSNNANDDDQR