Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32328.t1 | AFK49669.1 | 87.5 | 88 | 10 | 1 | 1 | 88 | 1 | 87 | 2.40E-31 | 144.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32328.t1 | Q67XJ2 | 41.4 | 111 | 62 | 1 | 3 | 110 | 29 | 139 | 1.5e-13 | 77.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32328.t1 | I3TAX7 | 87.5 | 88 | 10 | 1 | 1 | 88 | 1 | 87 | 1.7e-31 | 144.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene32328.t1 | TF | NF-YB |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32328.t1 | MTR_1g029100 | 84.615 | 91 | 13 | 1 | 1 | 91 | 1 | 90 | 4.40e-50 | 154 |
| MS.gene32328.t1 | MTR_1g072790 | 36.697 | 109 | 68 | 1 | 3 | 111 | 29 | 136 | 7.62e-22 | 85.1 |
| MS.gene32328.t1 | MTR_4g133952 | 38.889 | 90 | 55 | 0 | 3 | 92 | 58 | 147 | 3.90e-21 | 84.7 |
| MS.gene32328.t1 | MTR_3g058980 | 38.462 | 104 | 63 | 1 | 3 | 106 | 27 | 129 | 4.34e-21 | 83.2 |
| MS.gene32328.t1 | MTR_1g039040 | 40.816 | 98 | 57 | 1 | 3 | 100 | 6 | 102 | 6.52e-21 | 82.8 |
| MS.gene32328.t1 | MTR_7g061270 | 36.607 | 112 | 68 | 1 | 3 | 111 | 26 | 137 | 6.92e-21 | 82.4 |
| MS.gene32328.t1 | MTR_7g100650 | 36.607 | 112 | 68 | 1 | 3 | 111 | 26 | 137 | 1.14e-20 | 81.6 |
| MS.gene32328.t1 | MTR_1g088860 | 39.130 | 92 | 56 | 0 | 1 | 92 | 34 | 125 | 1.25e-20 | 82.4 |
| MS.gene32328.t1 | MTR_8g091720 | 38.679 | 106 | 64 | 1 | 3 | 108 | 28 | 132 | 1.66e-20 | 82.0 |
| MS.gene32328.t1 | MTR_5g095740 | 41.111 | 90 | 53 | 0 | 3 | 92 | 23 | 112 | 2.41e-20 | 81.3 |
| MS.gene32328.t1 | MTR_2g026710 | 40.426 | 94 | 55 | 1 | 3 | 96 | 17 | 109 | 2.93e-20 | 80.5 |
| MS.gene32328.t1 | MTR_4g119500 | 34.579 | 107 | 70 | 0 | 3 | 109 | 22 | 128 | 3.62e-20 | 79.3 |
| MS.gene32328.t1 | MTR_4g133938 | 40.230 | 87 | 52 | 0 | 6 | 92 | 22 | 108 | 4.36e-20 | 79.3 |
| MS.gene32328.t1 | MTR_0392s0020 | 36.667 | 90 | 57 | 0 | 3 | 92 | 18 | 107 | 2.11e-19 | 77.8 |
| MS.gene32328.t1 | MTR_1g029070 | 42.353 | 85 | 49 | 0 | 7 | 91 | 56 | 140 | 1.38e-18 | 76.6 |
| MS.gene32328.t1 | MTR_8g093920 | 40.449 | 89 | 53 | 0 | 3 | 91 | 40 | 128 | 1.47e-18 | 77.4 |
| MS.gene32328.t1 | MTR_1g083070 | 35.294 | 85 | 55 | 0 | 8 | 92 | 9 | 93 | 5.42e-18 | 73.9 |
| MS.gene32328.t1 | MTR_1g028480 | 38.372 | 86 | 39 | 1 | 39 | 110 | 25 | 110 | 8.31e-15 | 65.1 |
| MS.gene32328.t1 | MTR_5g095900 | 34.524 | 84 | 55 | 0 | 8 | 91 | 21 | 104 | 4.07e-14 | 65.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32328.t1 | AT3G53340 | 41.441 | 111 | 62 | 1 | 3 | 110 | 29 | 139 | 1.15e-23 | 89.7 |
| MS.gene32328.t1 | AT3G53340 | 41.441 | 111 | 62 | 1 | 3 | 110 | 29 | 139 | 1.15e-23 | 89.7 |
| MS.gene32328.t1 | AT2G38880 | 40.741 | 108 | 63 | 1 | 3 | 109 | 21 | 128 | 2.23e-23 | 87.8 |
| MS.gene32328.t1 | AT2G38880 | 40.000 | 110 | 63 | 1 | 3 | 109 | 21 | 130 | 4.30e-23 | 87.4 |
| MS.gene32328.t1 | AT2G37060 | 40.541 | 111 | 63 | 1 | 3 | 110 | 30 | 140 | 4.33e-23 | 88.2 |
| MS.gene32328.t1 | AT2G37060 | 40.541 | 111 | 63 | 1 | 3 | 110 | 30 | 140 | 4.33e-23 | 88.2 |
| MS.gene32328.t1 | AT2G37060 | 40.541 | 111 | 63 | 1 | 3 | 110 | 30 | 140 | 4.33e-23 | 88.2 |
| MS.gene32328.t1 | AT2G38880 | 40.000 | 110 | 63 | 1 | 3 | 109 | 21 | 130 | 4.81e-23 | 87.0 |
| MS.gene32328.t1 | AT2G38880 | 40.000 | 110 | 63 | 1 | 3 | 109 | 21 | 130 | 4.81e-23 | 87.0 |
| MS.gene32328.t1 | AT2G38880 | 40.000 | 110 | 63 | 1 | 3 | 109 | 21 | 130 | 4.81e-23 | 87.0 |
| MS.gene32328.t1 | AT2G38880 | 41.111 | 90 | 53 | 0 | 3 | 92 | 21 | 110 | 4.49e-21 | 81.3 |
| MS.gene32328.t1 | AT4G14540 | 41.111 | 90 | 53 | 0 | 3 | 92 | 21 | 110 | 6.11e-21 | 82.4 |
| MS.gene32328.t1 | AT5G47640 | 42.222 | 90 | 52 | 0 | 3 | 92 | 27 | 116 | 1.07e-20 | 82.4 |
| MS.gene32328.t1 | AT2G38880 | 41.111 | 90 | 53 | 0 | 3 | 92 | 21 | 110 | 1.35e-20 | 81.6 |
| MS.gene32328.t1 | AT2G38880 | 41.111 | 90 | 53 | 0 | 3 | 92 | 21 | 110 | 1.35e-20 | 81.6 |
| MS.gene32328.t1 | AT2G38880 | 41.111 | 90 | 53 | 0 | 3 | 92 | 21 | 110 | 2.29e-20 | 80.5 |
| MS.gene32328.t1 | AT2G38880 | 41.111 | 90 | 53 | 0 | 3 | 92 | 21 | 110 | 2.29e-20 | 80.5 |
| MS.gene32328.t1 | AT2G38880 | 41.111 | 90 | 53 | 0 | 3 | 92 | 21 | 110 | 2.29e-20 | 80.5 |
| MS.gene32328.t1 | AT2G47810 | 40.449 | 89 | 53 | 0 | 3 | 91 | 51 | 139 | 4.53e-20 | 80.1 |
| MS.gene32328.t1 | AT3G53340 | 42.222 | 90 | 52 | 0 | 3 | 92 | 29 | 118 | 7.70e-20 | 78.6 |
| MS.gene32328.t1 | AT5G47670 | 36.000 | 100 | 64 | 0 | 3 | 102 | 29 | 128 | 1.52e-19 | 79.7 |
| MS.gene32328.t1 | AT5G47670 | 36.000 | 100 | 64 | 0 | 3 | 102 | 58 | 157 | 3.59e-19 | 79.3 |
| MS.gene32328.t1 | AT5G47670 | 36.000 | 100 | 64 | 0 | 3 | 102 | 58 | 157 | 3.59e-19 | 79.3 |
| MS.gene32328.t1 | AT3G53340 | 42.222 | 90 | 52 | 0 | 3 | 92 | 29 | 118 | 5.45e-19 | 77.0 |
| MS.gene32328.t1 | AT3G53340 | 42.222 | 90 | 52 | 0 | 3 | 92 | 29 | 118 | 5.45e-19 | 77.0 |
| MS.gene32328.t1 | AT1G21970 | 37.778 | 90 | 56 | 0 | 3 | 92 | 59 | 148 | 5.67e-18 | 76.3 |
| MS.gene32328.t1 | AT2G13570 | 40.449 | 89 | 53 | 0 | 3 | 91 | 36 | 124 | 2.28e-17 | 74.3 |
| MS.gene32328.t1 | AT1G09030 | 31.522 | 92 | 63 | 0 | 1 | 92 | 1 | 92 | 1.49e-16 | 70.5 |
Find 26 sgRNAs with CRISPR-Local
Find 26 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGGAATCTGTTCGGTCTCT+TGG | 0.334557 | 1.4:+14197136 | MS.gene32328:CDS |
| TTTCCGCTGATGATTTACTA+TGG | 0.336830 | 1.4:+14197092 | MS.gene32328:CDS |
| ATTTACTATGGACGATGAAT+AGG | 0.363355 | 1.4:+14197104 | MS.gene32328:CDS |
| GATGCTCTACATTGGCTTGT+TGG | 0.407680 | 1.4:-14197060 | None:intergenic |
| AGGAATCTGTTCGGTCTCTT+GGG | 0.418982 | 1.4:+14197137 | MS.gene32328:CDS |
| GATGAATAGGTTGGGCTTTG+AGG | 0.427694 | 1.4:+14197117 | MS.gene32328:CDS |
| CTATGGACGATGAATAGGTT+GGG | 0.452990 | 1.4:+14197109 | MS.gene32328:CDS |
| TCAACGTTGGCCATCATCAT+TGG | 0.463278 | 1.4:-14197221 | None:intergenic |
| GACTCTTGTCACATGATTGA+TGG | 0.464195 | 1.4:-14196933 | None:intergenic |
| CGCAAGAACATGGATGGGTC+AGG | 0.479316 | 1.4:+14197178 | MS.gene32328:CDS |
| AACTATCGCAAGAACATGGA+TGG | 0.501574 | 1.4:+14197172 | MS.gene32328:CDS |
| TGGGCTTTGAGGAATCTGTT+CGG | 0.518806 | 1.4:+14197128 | MS.gene32328:CDS |
| TCATCAGTGATTATTGTGTC+TGG | 0.528332 | 1.4:-14196974 | None:intergenic |
| ACTATGGACGATGAATAGGT+TGG | 0.532041 | 1.4:+14197108 | MS.gene32328:CDS |
| AATAATCACTGATGATGCTA+AGG | 0.544983 | 1.4:+14196982 | MS.gene32328:CDS |
| ATTCTCTTGATGCTCTACAT+TGG | 0.555958 | 1.4:-14197068 | None:intergenic |
| TCAAACAATGCCAATGATGA+TGG | 0.560009 | 1.4:+14197211 | MS.gene32328:CDS |
| TTCAACTCCAGCAATGGGCG+AGG | 0.564498 | 1.4:+14196895 | None:intergenic |
| TGTGTCTAAATTTATGAACA+AGG | 0.573477 | 1.4:+14197021 | MS.gene32328:CDS |
| CATTTGTTTCATATCAACGT+TGG | 0.575876 | 1.4:-14197234 | None:intergenic |
| ATGGATGGGTCAGGTGCTAG+TGG | 0.576300 | 1.4:+14197187 | MS.gene32328:CDS |
| ACTCTTGTCACATGATTGAT+GGG | 0.580392 | 1.4:-14196932 | None:intergenic |
| TATGAACAAGGTCACAAGTG+AGG | 0.614031 | 1.4:+14197033 | MS.gene32328:CDS |
| TCAGTGATTATTGTGTCTGG+TGG | 0.634070 | 1.4:-14196971 | None:intergenic |
| ACTATCGCAAGAACATGGAT+GGG | 0.635414 | 1.4:+14197173 | MS.gene32328:CDS |
| CGTCCATAGTAAATCATCAG+CGG | 0.656317 | 1.4:-14197095 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TGTGTCTAAATTTATGAACA+AGG | + | chr1.4:14197021-14197040 | MS.gene32328:CDS | 25.0% |
| ATTTACTATGGACGATGAAT+AGG | + | chr1.4:14197104-14197123 | MS.gene32328:CDS | 30.0% | |
| ! | AATAATCACTGATGATGCTA+AGG | + | chr1.4:14196982-14197001 | MS.gene32328:CDS | 30.0% |
| ACTCTTGTCACATGATTGAT+GGG | - | chr1.4:14196935-14196954 | None:intergenic | 35.0% | |
| ATTCTCTTGATGCTCTACAT+TGG | - | chr1.4:14197071-14197090 | None:intergenic | 35.0% | |
| CAAAAACTATCGCAAGAACA+TGG | + | chr1.4:14197168-14197187 | MS.gene32328:CDS | 35.0% | |
| TCAAACAATGCCAATGATGA+TGG | + | chr1.4:14197211-14197230 | MS.gene32328:CDS | 35.0% | |
| TCATCAGTGATTATTGTGTC+TGG | - | chr1.4:14196977-14196996 | None:intergenic | 35.0% | |
| TTTCCGCTGATGATTTACTA+TGG | + | chr1.4:14197092-14197111 | MS.gene32328:CDS | 35.0% | |
| ! | TGTTCTTGCGATAGTTTTTG+AGG | - | chr1.4:14197168-14197187 | None:intergenic | 35.0% |
| AACTATCGCAAGAACATGGA+TGG | + | chr1.4:14197172-14197191 | MS.gene32328:CDS | 40.0% | |
| ACTATCGCAAGAACATGGAT+GGG | + | chr1.4:14197173-14197192 | MS.gene32328:CDS | 40.0% | |
| ACTATGGACGATGAATAGGT+TGG | + | chr1.4:14197108-14197127 | MS.gene32328:CDS | 40.0% | |
| CGTCCATAGTAAATCATCAG+CGG | - | chr1.4:14197098-14197117 | None:intergenic | 40.0% | |
| CTATGGACGATGAATAGGTT+GGG | + | chr1.4:14197109-14197128 | MS.gene32328:CDS | 40.0% | |
| GACTCTTGTCACATGATTGA+TGG | - | chr1.4:14196936-14196955 | None:intergenic | 40.0% | |
| TATGAACAAGGTCACAAGTG+AGG | + | chr1.4:14197033-14197052 | MS.gene32328:CDS | 40.0% | |
| TCAGTGATTATTGTGTCTGG+TGG | - | chr1.4:14196974-14196993 | None:intergenic | 40.0% | |
| AGGAATCTGTTCGGTCTCTT+GGG | + | chr1.4:14197137-14197156 | MS.gene32328:CDS | 45.0% | |
| TCAACGTTGGCCATCATCAT+TGG | - | chr1.4:14197224-14197243 | None:intergenic | 45.0% | |
| ! | GATGAATAGGTTGGGCTTTG+AGG | + | chr1.4:14197117-14197136 | MS.gene32328:CDS | 45.0% |
| !! | GATGCTCTACATTGGCTTGT+TGG | - | chr1.4:14197063-14197082 | None:intergenic | 45.0% |
| !! | TGGGCTTTGAGGAATCTGTT+CGG | + | chr1.4:14197128-14197147 | MS.gene32328:CDS | 45.0% |
| GAGGAATCTGTTCGGTCTCT+TGG | + | chr1.4:14197136-14197155 | MS.gene32328:CDS | 50.0% | |
| ATGGATGGGTCAGGTGCTAG+TGG | + | chr1.4:14197187-14197206 | MS.gene32328:CDS | 55.0% | |
| CGCAAGAACATGGATGGGTC+AGG | + | chr1.4:14197178-14197197 | MS.gene32328:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 14196908 | 14197243 | 14196908 | ID=MS.gene32328 |
| chr1.4 | mRNA | 14196908 | 14197243 | 14196908 | ID=MS.gene32328.t1;Parent=MS.gene32328 |
| chr1.4 | exon | 14196908 | 14197243 | 14196908 | ID=MS.gene32328.t1.exon1;Parent=MS.gene32328.t1 |
| chr1.4 | CDS | 14196908 | 14197243 | 14196908 | ID=cds.MS.gene32328.t1;Parent=MS.gene32328.t1 |
| Gene Sequence |
| Protein sequence |
