AlfalfaGEDB | MS.gene32331

M. sativa cultivar XinJiangDaYe / MS.gene32331

KEGG: K08065
GO: GO:0003674 GO:0005488 GO:0005515 GO:0046982 GO:0046983
PFAM: PF00808.26

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene32331.t1	XP_013466407.1	91.9	172	14	0	1	172	1	172	2.60E-77	298.1

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene32331.t1	O23310	52.4	105	50	0	50	154	19	123	1.3e-24	114.4

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene32331.t1	A0A072VGA7	91.9	172	14	0	1	172	1	172	1.9e-77	298.1

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene32331.t1	TF	NF-YB

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene32331.t1	MTR_1g029070	91.860	172	14	0	1	172	1	172	2.59e-104	296
MS.gene32331.t1	MTR_1g072790	47.287	129	60	2	48	172	25	149	1.13e-35	122
MS.gene32331.t1	MTR_8g091720	55.670	97	43	0	57	153	33	129	1.24e-34	120
MS.gene32331.t1	MTR_1g039040	57.303	89	38	0	57	145	11	99	2.52e-34	119
MS.gene32331.t1	MTR_7g061270	45.600	125	54	2	48	169	19	132	5.28e-34	118
MS.gene32331.t1	MTR_7g100650	44.615	130	58	2	43	169	14	132	5.60e-34	118
MS.gene32331.t1	MTR_5g095740	49.123	114	53	2	46	159	18	126	9.11e-33	115
MS.gene32331.t1	MTR_2g026710	56.044	91	40	0	57	147	22	112	1.59e-32	114
MS.gene32331.t1	MTR_3g058980	52.000	100	47	1	46	145	22	120	5.01e-32	114
MS.gene32331.t1	MTR_4g133952	40.441	136	80	1	9	144	16	150	3.72e-31	112

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene32331.t1	AT4G14540	51.402	107	52	0	48	154	17	123	7.81e-35	120
MS.gene32331.t1	AT2G38880	50.893	112	51	2	57	168	26	133	1.76e-33	115
MS.gene32331.t1	AT5G47640	52.941	102	47	1	47	148	23	123	2.64e-33	117
MS.gene32331.t1	AT3G53340	41.429	140	59	2	30	169	19	135	2.87e-33	116
MS.gene32331.t1	AT3G53340	41.429	140	59	2	30	169	19	135	2.87e-33	116
MS.gene32331.t1	AT2G38880	49.558	113	53	2	57	168	26	135	3.75e-33	115
MS.gene32331.t1	AT2G38880	49.558	113	53	2	57	168	26	135	4.30e-33	115
MS.gene32331.t1	AT2G38880	49.558	113	53	2	57	168	26	135	4.30e-33	115
MS.gene32331.t1	AT2G38880	49.558	113	53	2	57	168	26	135	4.30e-33	115
MS.gene32331.t1	AT2G37060	46.552	116	59	1	57	172	35	147	5.12e-33	115

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Find 46 sgRNAs with CRISPR-Local

Find 99 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACATGGATGGGTCGGGTGC+TGG	0.425049	1.4:+14178545	MS.gene32331:CDS
AACCATGAGGCTCAATTCAG+TGG	0.593599	1.4:+14178500	MS.gene32331:CDS
AACTGCTCGTGGATTCAACA+TGG	0.604751	1.4:+14178529	MS.gene32331:CDS
ACACAATTGTGTTGTTGCCT+TGG	0.463454	1.4:-14178318	None:intergenic
AGTGGCACGTCAACTGCTCG+TGG	0.583180	1.4:+14178518	MS.gene32331:CDS
ATATAATATGGGCCATGGGT+AGG	0.601954	1.4:+14178423	MS.gene32331:CDS
ATATGGGCCATGGGTAGGTT+AGG	0.348171	1.4:+14178428	MS.gene32331:CDS
ATGAAAACAATGAATCTCCA+AGG	0.596844	1.4:+14178159	MS.gene32331:CDS
ATGGACTCCCTGCTTGCAAA+CGG	0.525534	1.4:-14178136	None:intergenic
ATGGATGGGTCGGGTGCTGG+TGG	0.486211	1.4:+14178548	MS.gene32331:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAATATCTCAAGATGCCA+AGG	+	chr1.4:14178301-14178320	MS.gene32331:CDS	30.0%
AAATAAAGTGAGAGAAAAGG+TGG	+	chr1.4:14177684-14177703	MS.gene32331:intron	30.0%
AAATTGAGAGAAAGAGATAG+AGG	-	chr1.4:14177382-14177401	None:intergenic	30.0%
AATAAAGTGAGAGAAAAGGT+GGG	+	chr1.4:14177685-14177704	MS.gene32331:intron	30.0%
ATGAAAACAATGAATCTCCA+AGG	+	chr1.4:14178159-14178178	MS.gene32331:CDS	30.0%
ATTCTTGTCAAATTTCGGAT+GGG	-	chr1.4:14178254-14178273	None:intergenic	30.0%
GAAATTTGACAAGAATCATG+CGG	+	chr1.4:14178258-14178277	MS.gene32331:CDS	30.0%
GCAAAATTGACGTATTTAAG+GGG	-	chr1.4:14177729-14177748	None:intergenic	30.0%
GCATGATTCTTGTCAAATTT+CGG	-	chr1.4:14178259-14178278	None:intergenic	30.0%
TGTCAATTATACGTTACGTT+CGG	+	chr1.4:14177961-14177980	MS.gene32331:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1.4	gene	14176876	14178598	14176876	ID=MS.gene32331
chr1.4	mRNA	14176876	14178598	14176876	ID=MS.gene32331.t1;Parent=MS.gene32331
chr1.4	exon	14176876	14176930	14176876	ID=MS.gene32331.t1.exon1;Parent=MS.gene32331.t1
chr1.4	CDS	14176876	14176930	14176876	ID=cds.MS.gene32331.t1;Parent=MS.gene32331.t1
chr1.4	exon	14178135	14178598	14178135	ID=MS.gene32331.t1.exon2;Parent=MS.gene32331.t1
chr1.4	CDS	14178135	14178598	14178135	ID=cds.MS.gene32331.t1;Parent=MS.gene32331.t1

Gene Sequence

>MS.gene32331

ATGGAGCATGGAAGTCCCTCATTTAACAACAACCGTTCAGCACAATCAAGCTCTGGTAAGCACTCTAAACAATAAACAAAAAAATTACACCCTTGTATCTCTTTATAATGAGTTGAGATCCTCGTATTTCTCTAAAATAACAGAGAGTAATGATATTTAAAAAGAGTGATTAACACATATTTTAATTTTTAAAATTTTTTTACCTTGTAAAAGATTGATTTTGATATCTAATTTCATAGTAGATCTTACTGTTTTAATATTTTTCGCTAGATTTTATTTTATTTTTCGTATTTTTCCCCCTTAATTGATTTTATTAGATTCTCAAGGCATTATTTGCTTCGGAAAGAAAGATCTAACTTGGGATATGATTGTGCATCACTTTTTTTTTTGAAGGAGATTGTGCATCACTTGTTAGCGATTTTTTACACTGGAAAATACTTAGTTATTTAAAGAAATTTTGATTTAAGGGGATGACAAAAAAAAAATATGGATCTCATATTTGACCTCTATCTCTTTCTCTCAATTTTATATATATCTTTAATATTTTTAATTTATTATTTTAATATGTCTGAGGGTTATAAATTTTGGGGTACCGAATTCGATTCACACTAAAAAAGATTTGAGATCTTGGAATTAATTTTATTCAGAGCAGATCTGCGTATACAATGGTGAGAAAAACATCTTTGCTAAGATTTCAATTTTTTAATATTATAAATTATTGTATTTAAAACTAATGGGATATAATATTAATTATAATAATAAATTAATTATTTGAGGTTACACAAAAATGCAATTATCGTGTGAATAGAAATAAAGTGAGAGAAAAGGTGGGTGTCAAAATAAACAAGGTCCCCTTAAATACGTCAATTTTGCTACAAATTTCATTGTTGATCTATCTAATGGTGTGAAGCTAACCGGTATCTACACAGACACAAAAAATATGTTTGTAATATATAGCTTCATAGTTCTCGTTTTTATATGTAAAGATTCCACTAACGTCAAGTGATGATGTGTAGTCACCTTTTACATGCTGGTTTGCCACCTTAGATTTAAAAGTAGAAAAAAAATTAATACATTACATTTTTTGTCAATTATACGTTACGTTCGGACTTTGACACTCTGTGTAATAAAAAAGAAGGAAAGAAAGTATACAATATACACTGTATTTTTAATGTAAATTACTCTTATGTTAAATATTTTTTCAATAAAAAATTATTTTCATGTAAATTACTTAACTACGTGCTTATGTTTGGATTTAGTCCGTTTGCAAGCAGGGAGTCCATATGAAAACAATGAATCTCCAAGGACCGAAAACATTGATGCTGAAATTGAGAAACAAGATTTATCTCCAACAACAAATGAGGAACTGTGTCTTCCCATCCGAAATTTGACAAGAATCATGCGGCGTGCTCTTCCATCGTACGCAAAAATATCTCAAGATGCCAAGGCAACAACACAATTGTGTGTGTCTGATTTCATGGGCATCATCACCACTGAGGCTGTTGAGAAATGCAATGAAGAGAGTCGAAAGATCATTAGTGGTGAAGATATAATATGGGCCATGGGTAGGTTAGGGTTTGAGGACTATGTTGGTCCTCTATCTCTTCTCCTCCAAAACTATCGCAACCATGAGGCTCAATTCAGTGGCACGTCAACTGCTCGTGGATTCAACATGGATGGGTCGGGTGCTGGTGGCTCGAGCAGTGGTAATGGCCAATATTGA

Protein sequence

>MS.gene32331.t1

MEHGSPSFNNNRSAQSSSVRLQAGSPYENNESPRTENIDAEIEKQDLSPTTNEELCLPIRNLTRIMRRALPSYAKISQDAKATTQLCVSDFMGIITTEAVEKCNEESRKIISGEDIIWAMGRLGFEDYVGPLSLLLQNYRNHEAQFSGTSTARGFNMDGSGAGGSSSGNGQY