AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene32353

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32353.t1 MTR_0402s0040 74.634 205 51 1 9 213 1 204 5.35e-102 317
MS.gene32353.t1 MTR_0402s0040 67.196 189 48 3 365 553 318 492 8.66e-77 251
MS.gene32353.t1 MTR_7g113010 83.019 159 13 1 1 159 424 568 1.06e-84 275
MS.gene32353.t1 MTR_1g057920 95.122 123 6 0 1 123 1 123 5.92e-82 252
MS.gene32353.t1 MTR_6g017200 78.049 123 27 0 8 130 32 154 1.26e-62 203
MS.gene32353.t1 MTR_5g015480 60.736 163 60 1 1 163 27 185 1.69e-57 193
MS.gene32353.t1 MTR_2g070590 73.504 117 31 0 20 136 3 119 3.63e-53 177
MS.gene32353.t1 MTR_0558s0020 34.677 248 141 5 48 275 50 296 2.71e-44 159
MS.gene32353.t1 MTR_6g051425 28.713 303 186 7 36 315 19 314 5.19e-39 146
MS.gene32353.t1 MTR_8g446020 35.577 208 121 3 80 275 50 256 1.39e-38 142
MS.gene32353.t1 MTR_6g090505 35.849 212 119 4 79 274 20 230 3.08e-36 139
MS.gene32353.t1 MTR_1g050985 93.333 60 4 0 104 163 39 98 2.96e-33 122
MS.gene32353.t1 MTR_0137s0040 56.731 104 43 1 1 102 1 104 1.38e-30 115
MS.gene32353.t1 MTR_1g083640 30.131 229 139 5 38 246 55 282 1.78e-29 118
MS.gene32353.t1 MTR_1g070355 30.120 249 160 6 29 269 67 309 1.13e-28 120
MS.gene32353.t1 MTR_0291s0060 68.919 74 23 0 11 84 12 85 9.07e-27 104
MS.gene32353.t1 MTR_4g035820 29.167 264 174 8 25 279 65 324 1.48e-24 108
MS.gene32353.t1 MTR_7g074790 37.143 175 82 5 34 204 10 160 2.85e-23 99.0
MS.gene32353.t1 MTR_0337s0040 30.636 173 107 3 80 240 51 222 2.68e-22 96.3
MS.gene32353.t1 MTR_7g446520 29.508 244 154 4 38 280 92 318 1.84e-21 96.3
MS.gene32353.t1 MTR_5g076070 27.119 236 154 7 37 263 56 282 7.85e-21 93.2
MS.gene32353.t1 MTR_7g446480 35.227 176 111 2 110 283 140 314 8.28e-21 94.0
MS.gene32353.t1 MTR_5g039760 33.668 199 100 6 36 230 12 182 9.14e-21 90.5
MS.gene32353.t1 MTR_7g446510 34.659 176 112 2 110 283 155 329 1.28e-19 90.5
MS.gene32353.t1 MTR_4g067290 41.593 113 60 3 28 138 50 158 4.70e-18 84.0
MS.gene32353.t1 MTR_4g067290 41.593 113 60 3 28 138 50 158 5.52e-18 84.0
MS.gene32353.t1 MTR_4g067290 41.593 113 60 3 28 138 70 178 7.94e-18 84.0
MS.gene32353.t1 MTR_4g067290 41.593 113 60 3 28 138 70 178 8.19e-18 84.0
MS.gene32353.t1 MTR_4g067290 41.593 113 60 3 28 138 68 176 8.21e-18 84.0
MS.gene32353.t1 MTR_1g008290 34.752 141 86 3 89 228 6 141 1.50e-17 80.1
MS.gene32353.t1 MTR_4g067290 41.593 113 60 3 28 138 110 218 1.64e-17 84.0
MS.gene32353.t1 MTR_4g102720 29.762 168 82 3 34 201 7 138 3.52e-17 81.3
MS.gene32353.t1 MTR_7g061980 39.823 113 64 3 34 145 10 119 6.77e-17 77.4
MS.gene32353.t1 MTR_2g098920 40.187 107 58 3 34 138 80 182 1.74e-16 80.1
MS.gene32353.t1 MTR_2g014100 23.737 198 133 3 38 217 48 245 2.25e-16 79.3
MS.gene32353.t1 MTR_1g019850 32.903 155 95 2 14 167 50 196 3.73e-16 78.6
MS.gene32353.t1 MTR_3g006370 39.048 105 62 2 34 137 50 153 2.55e-15 75.9
MS.gene32353.t1 MTR_3g450850 24.603 252 172 6 37 274 50 297 8.66e-15 76.3
MS.gene32353.t1 MTR_0019s0290 28.873 142 89 2 83 213 19 159 9.32e-15 72.4
MS.gene32353.t1 MTR_1g055240 28.571 147 93 2 83 218 4 149 3.49e-14 70.9
MS.gene32353.t1 MTR_5g045210 39.423 104 59 2 334 437 9 108 1.53e-13 70.1
MS.gene32353.t1 MTR_1g091013 46.753 77 30 1 26 102 4 69 6.41e-13 64.3
MS.gene32353.t1 MTR_2g098880 32.624 141 84 3 34 172 73 204 1.85e-12 68.2
MS.gene32353.t1 MTR_2g098880 32.624 141 84 3 34 172 61 192 2.14e-12 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32353.t1 AT4G38180 38.202 356 211 6 1 350 39 391 9.56e-74 250
MS.gene32353.t1 AT4G15090 32.628 331 214 5 34 362 51 374 1.89e-54 197
MS.gene32353.t1 AT4G15090 32.628 331 214 5 34 362 51 374 1.89e-54 197
MS.gene32353.t1 AT4G15090 32.628 331 214 5 34 362 51 374 1.89e-54 197
MS.gene32353.t1 AT4G15090 32.628 331 214 5 34 362 51 374 1.89e-54 197
MS.gene32353.t1 AT4G15090 32.628 331 214 5 34 362 51 374 1.89e-54 197
MS.gene32353.t1 AT1G76320 33.544 316 197 6 38 351 1 305 2.63e-51 187
MS.gene32353.t1 AT1G76320 33.544 316 197 6 38 351 1 305 4.77e-51 187
MS.gene32353.t1 AT1G76320 33.544 316 197 6 38 351 1 305 4.77e-51 187
MS.gene32353.t1 AT1G76320 33.544 316 197 6 38 351 1 305 5.20e-51 187
MS.gene32353.t1 AT1G10240 31.373 357 225 8 2 350 25 369 1.88e-50 184
MS.gene32353.t1 AT1G10240 31.373 357 225 8 2 350 25 369 1.88e-50 184
MS.gene32353.t1 AT3G22170 29.290 338 200 6 27 350 64 376 9.88e-43 164
MS.gene32353.t1 AT3G22170 29.290 338 200 6 27 350 64 376 9.88e-43 164
MS.gene32353.t1 AT3G06250 29.730 333 191 8 34 363 190 482 1.61e-42 163
MS.gene32353.t1 AT3G06250 33.036 112 61 2 25 136 19 116 2.68e-11 67.0
MS.gene32353.t1 AT3G06250 29.730 333 191 8 34 363 190 482 1.61e-42 163
MS.gene32353.t1 AT3G06250 33.036 112 61 2 25 136 19 116 2.68e-11 67.0
MS.gene32353.t1 AT3G06250 29.730 333 191 8 34 363 190 482 1.61e-42 163
MS.gene32353.t1 AT3G06250 33.036 112 61 2 25 136 19 116 2.68e-11 67.0
MS.gene32353.t1 AT2G27110 31.014 345 203 10 8 350 27 338 2.62e-42 162
MS.gene32353.t1 AT2G27110 31.014 345 203 10 8 350 27 338 2.62e-42 162
MS.gene32353.t1 AT5G18960 29.532 342 198 9 23 359 197 500 9.67e-40 155
MS.gene32353.t1 AT5G18960 33.613 119 65 2 18 136 27 131 1.37e-13 74.3
MS.gene32353.t1 AT5G18960 29.532 342 198 9 23 359 197 500 9.67e-40 155
MS.gene32353.t1 AT5G18960 33.613 119 65 2 18 136 27 131 1.37e-13 74.3
MS.gene32353.t1 AT5G28530 27.964 329 210 8 34 342 55 376 2.83e-38 150
MS.gene32353.t1 AT5G28530 27.964 329 210 8 34 342 55 376 2.92e-38 150
MS.gene32353.t1 AT5G28530 27.964 329 210 8 34 342 55 376 2.92e-38 150
MS.gene32353.t1 AT2G32250 28.615 325 185 7 37 359 41 320 1.40e-37 148
MS.gene32353.t1 AT2G32250 28.615 325 185 7 37 359 41 320 1.40e-37 148
MS.gene32353.t1 AT2G32250 28.615 325 185 7 37 359 41 320 1.43e-37 148
MS.gene32353.t1 AT2G32250 28.615 325 185 7 37 359 41 320 1.63e-37 148
MS.gene32353.t1 AT2G32250 28.615 325 185 7 37 359 41 320 1.63e-37 148
MS.gene32353.t1 AT2G32250 28.615 325 185 7 37 359 41 320 1.63e-37 148
MS.gene32353.t1 AT2G32250 27.864 323 188 6 38 359 1 279 1.00e-36 145
MS.gene32353.t1 AT2G32250 27.864 323 188 6 38 359 1 279 1.12e-36 145
MS.gene32353.t1 AT1G80010 29.032 341 226 8 19 350 80 413 8.93e-34 137
MS.gene32353.t1 AT1G52520 30.233 344 190 13 33 360 84 393 3.81e-33 135
MS.gene32353.t1 AT4G19990 28.274 336 189 7 37 357 24 322 4.20e-33 135
MS.gene32353.t1 AT4G19990 28.274 336 189 7 37 357 24 322 4.20e-33 135
MS.gene32353.t1 AT4G19990 28.274 336 189 7 37 357 24 322 7.26e-33 134
MS.gene32353.t1 AT4G19990 28.274 336 189 7 37 357 24 322 8.27e-33 134
MS.gene32353.t1 AT4G19990 28.274 336 189 7 37 357 24 322 8.27e-33 134
MS.gene32353.t1 AT4G19990 28.274 336 189 7 37 357 38 336 8.71e-33 134
MS.gene32353.t1 AT2G27110 42.446 139 79 1 213 350 55 193 9.80e-31 127
MS.gene32353.t1 AT4G19990 27.667 300 165 7 73 357 3 265 7.48e-25 109
MS.gene32353.t1 AT4G38170 37.594 133 77 3 210 340 18 146 9.12e-24 105
MS.gene32353.t1 AT5G28530 24.127 315 177 10 34 342 55 313 8.88e-20 93.6
MS.gene32353.t1 AT5G28530 24.127 315 177 10 34 342 55 313 1.06e-19 93.2
MS.gene32353.t1 AT3G59470 41.905 105 59 2 34 138 70 172 3.78e-18 84.7
MS.gene32353.t1 AT3G59470 41.905 105 59 2 34 138 70 172 3.78e-18 84.7
MS.gene32353.t1 AT3G59470 41.905 105 59 2 34 138 82 184 5.91e-18 84.3
MS.gene32353.t1 AT3G07500 40.952 105 61 1 34 138 33 136 1.15e-16 79.7
MS.gene32353.t1 AT2G43280 39.130 115 66 3 34 145 22 135 6.47e-16 77.0
MS.gene32353.t1 AT4G12850 35.714 126 74 3 34 159 11 129 5.57e-15 72.8
MS.gene32353.t1 AT4G12850 38.835 103 59 2 34 136 11 109 8.07e-14 69.3
MS.gene32353.t1 AT4G12850 38.835 103 59 2 34 136 11 109 1.68e-13 69.7
MS.gene32353.t1 AT2G43280 38.000 100 58 3 49 145 2 100 2.76e-12 65.9

Find 86 sgRNAs with CRISPR-Local

Find 120 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCTAATAACAAATATATTT+TGG 0.090860 1.4:+13731984 MS.gene32353:CDS
TTCCAGATTGTGTCGATAAA+TGG 0.224458 1.4:+13731294 MS.gene32353:CDS
TTCATCATTCGCAATTGATA+TGG 0.254192 1.4:-13732733 None:intergenic
GTTGAGTTCCATTGTTTAAT+TGG 0.261409 1.4:-13733071 None:intergenic
CTTTAGTGATTGCTTCCTTT+TGG 0.266356 1.4:-13731873 None:intergenic
TTTGGTATTCGTCGAGAATA+TGG 0.287007 1.4:+13731452 MS.gene32353:CDS
GCACAGATGATTAATGATTA+TGG 0.297180 1.4:+13732013 MS.gene32353:CDS
AATGATTACAGTAAAAGAAT+TGG 0.319753 1.4:+13731428 MS.gene32353:CDS
ATGAGATTCTGAAGCTCTAT+TGG 0.322184 1.4:-13732601 None:intergenic
TACAGTAAAAGAATTGGATT+TGG 0.322823 1.4:+13731434 MS.gene32353:CDS
ATTGTCTTGGGAGATGAATC+TGG 0.337532 1.4:+13731752 MS.gene32353:CDS
CTAAAAGACGTTATAAATCT+TGG 0.340563 1.4:+13732823 MS.gene32353:CDS
CATTGTACCGCTTCATGAAA+TGG 0.342244 1.4:-13732551 None:intergenic
CACCACTTACAAGACAAATA+AGG 0.342570 1.4:+13732063 MS.gene32353:CDS
ACCCATTTATCGACACAATC+TGG 0.348105 1.4:-13731296 None:intergenic
CGACCTTTGGGTGTATTTGT+TGG 0.350273 1.4:+13732091 MS.gene32353:CDS
AAATAAGGATTATCGACCTT+TGG 0.372421 1.4:+13732078 MS.gene32353:CDS
GGAAATAAAAGCAGAATAGA+TGG 0.381196 1.4:+13731473 MS.gene32353:CDS
TCGAAAGATAGGGAAATATA+AGG 0.398025 1.4:+13731628 MS.gene32353:CDS
ATCTCACTTGATCGAAAGAT+AGG 0.398426 1.4:+13731617 MS.gene32353:CDS
TGGTTTAGTACAATTGTTAC+CGG 0.405047 1.4:+13732938 MS.gene32353:CDS
AGGAAGCAATCACTAAAGTA+TGG 0.405760 1.4:+13731878 MS.gene32353:CDS
GCTGGACTCTTCATAAACTT+TGG 0.408472 1.4:-13732643 None:intergenic
CTCCCATCGACACATATCTA+AGG 0.410986 1.4:+13731715 MS.gene32353:CDS
AATAAGGATTATCGACCTTT+GGG 0.412280 1.4:+13732079 MS.gene32353:CDS
ATCCTTATTTGTCTTGTAAG+TGG 0.412387 1.4:-13732065 None:intergenic
TGCCAATAGTGGACCTCACT+TGG 0.426768 1.4:+13731364 MS.gene32353:CDS
TTGTTGGACTTAATAATCAT+AGG 0.427337 1.4:+13732107 MS.gene32353:CDS
ATTAATGATTATGGATACTT+TGG 0.430083 1.4:+13732022 MS.gene32353:CDS
TCCCAAGTGAGGTCCACTAT+TGG 0.441740 1.4:-13731366 None:intergenic
ATTGTACCGCTTCATGAAAT+GGG 0.445421 1.4:-13732550 None:intergenic
TCAAATAACCACTGAAAAGA+AGG 0.449244 1.4:-13732175 None:intergenic
TCCAGATTGTGTCGATAAAT+GGG 0.451957 1.4:+13731295 MS.gene32353:CDS
GAAATTACAATGAGTTAACT+TGG 0.462389 1.4:-13733125 None:intergenic
GACACTGTTGGCGAGGCAAA+AGG 0.467223 1.4:+13732771 MS.gene32353:CDS
TGCATCTTGATCTGTTAGAA+TGG 0.469730 1.4:-13732236 None:intergenic
AAAATTTGCTTAAAACCAAA+AGG 0.473219 1.4:+13731858 MS.gene32353:CDS
AAAGAAGGTATTGCCAATAG+TGG 0.477439 1.4:+13731353 MS.gene32353:CDS
GGAAATAATCAAGGTTGGAG+TGG 0.484406 1.4:+13732501 MS.gene32353:CDS
TTGAAAATCATAAAAGAATG+AGG 0.485720 1.4:-13731948 None:intergenic
TTGTTTGAAATATGTCATCA+AGG 0.487222 1.4:-13731912 None:intergenic
AACAGGATGCACAGCGCGTA+TGG 0.489143 1.4:+13731592 MS.gene32353:CDS
ATTCCACTTGATTCTCCATC+CGG 0.489687 1.4:-13732699 None:intergenic
GAAATAATCAAGGTTGGAGT+GGG 0.495856 1.4:+13732502 MS.gene32353:CDS
ATATATTGATGCAAATCTCT+TGG 0.499334 1.4:-13731782 None:intergenic
TGGACAAGACAGGCAAGGTT+GGG 0.500158 1.4:+13732480 MS.gene32353:CDS
GCTGGTGGGGTAGAGATTGT+CGG 0.503945 1.4:+13731815 MS.gene32353:CDS
ATGGACAAGACAGGCAAGGT+TGG 0.516155 1.4:+13732479 MS.gene32353:CDS
TCTAGAGCAGAAACAAGAAC+AGG 0.521464 1.4:+13731575 MS.gene32353:CDS
AATGTTCATCATGAGAAAGA+AGG 0.529094 1.4:+13731338 MS.gene32353:CDS
CATACTCAGGTGGAATAAGA+AGG 0.556614 1.4:-13731679 None:intergenic
TGAAAATCATAAAAGAATGA+GGG 0.563844 1.4:-13731947 None:intergenic
GGGAATGAAGTTTGAATCTG+AGG 0.567573 1.4:+13731385 MS.gene32353:CDS
GTGCCTTAGATATGTGTCGA+TGG 0.567824 1.4:-13731718 None:intergenic
GCAAGGTTGGGAAATAATCA+AGG 0.568892 1.4:+13732492 MS.gene32353:CDS
GCCAATAGTGGACCTCACTT+GGG 0.571514 1.4:+13731365 MS.gene32353:CDS
AACAGATCAAGATGCAGCAA+TGG 0.573110 1.4:+13732243 MS.gene32353:CDS
AACACTACAGACTAAAACGA+TGG 0.576214 1.4:+13732460 MS.gene32353:CDS
GGTTGGGAAATAATCAAGGT+TGG 0.576405 1.4:+13732496 MS.gene32353:CDS
TATATCTAAGCAAGCTGGTG+GGG 0.581179 1.4:+13731802 MS.gene32353:CDS
ATAATCAAGGTTGGAGTGGG+AGG 0.581542 1.4:+13732505 MS.gene32353:CDS
CGAATCATATGAACATACTC+AGG 0.583230 1.4:-13731692 None:intergenic
TCTCACTTGATCGAAAGATA+GGG 0.583267 1.4:+13731618 MS.gene32353:CDS
TATATATCTAAGCAAGCTGG+TGG 0.584280 1.4:+13731800 MS.gene32353:CDS
CAATATATATCTAAGCAAGC+TGG 0.586937 1.4:+13731797 MS.gene32353:CDS
TGCCTTAGATATGTGTCGAT+GGG 0.595757 1.4:-13731717 None:intergenic
TTGGTCAATTTAAGTAGCTG+AGG 0.600722 1.4:-13732582 None:intergenic
ATATATCTAAGCAAGCTGGT+GGG 0.601684 1.4:+13731801 MS.gene32353:CDS
CAACAAAGACACTGTTGGCG+AGG 0.606814 1.4:+13732764 MS.gene32353:CDS
GGATGGAGAATCAAGTGGAA+TGG 0.607473 1.4:+13732701 MS.gene32353:CDS
TCTGCTCTAGACTCCGCCGT+CGG 0.612404 1.4:-13731563 None:intergenic
TCAAACTTCATTCCCAAGTG+AGG 0.614294 1.4:-13731377 None:intergenic
GACAACAACAAAGACACTGT+TGG 0.616614 1.4:+13732759 MS.gene32353:CDS
ACTAAAACGATGGACAAGAC+AGG 0.619879 1.4:+13732470 MS.gene32353:CDS
TATCCGGATGGAGAATCAAG+TGG 0.622649 1.4:+13732696 MS.gene32353:CDS
AGTCCAACAAATACACCCAA+AGG 0.632966 1.4:-13732094 None:intergenic
CAATTAAGCCAATTAAACAA+TGG 0.642422 1.4:+13733063 MS.gene32353:CDS
AAGATTCACATGCTTCAAAG+AGG 0.642884 1.4:+13731511 MS.gene32353:CDS
GGCAAAAGGCATAAAGACGA+AGG 0.643397 1.4:+13732785 MS.gene32353:CDS
AACGATGGACAAGACAGGCA+AGG 0.644015 1.4:+13732475 MS.gene32353:CDS
ATCATATGAACATACTCAGG+TGG 0.645731 1.4:-13731689 None:intergenic
AGATTCACATGCTTCAAAGA+GGG 0.654356 1.4:+13731512 MS.gene32353:CDS
TGACAAAAGACGTCAACCGA+CGG 0.667263 1.4:+13731547 MS.gene32353:CDS
AATGAAGTTTGAATCTGAGG+CGG 0.671588 1.4:+13731388 MS.gene32353:CDS
ACTCAGGTGGAATAAGAAGG+TGG 0.694755 1.4:-13731676 None:intergenic
CAAAAGACGTCAACCGACGG+CGG 0.706901 1.4:+13731550 MS.gene32353:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAAAAGTTAAATAAATGAT+AGG - chr1.4:13733004-13733023 None:intergenic 15.0%
!!! AGCTAATAACAAATATATTT+TGG + chr1.4:13731984-13732003 MS.gene32353:CDS 15.0%
!!! ATTCTTTTATGATTTTCAAA+TGG + chr1.4:13731952-13731971 MS.gene32353:CDS 15.0%
!!! GCTAATAACAAATATATTTT+GGG + chr1.4:13731985-13732004 MS.gene32353:CDS 15.0%
!! AAATTGAAAAAATCTCAAGA+TGG + chr1.4:13732918-13732937 MS.gene32353:CDS 20.0%
!! AATGATTACAGTAAAAGAAT+TGG + chr1.4:13731428-13731447 MS.gene32353:CDS 20.0%
!! ATTAATGATTATGGATACTT+TGG + chr1.4:13732022-13732041 MS.gene32353:CDS 20.0%
!! TGAAAATCATAAAAGAATGA+GGG - chr1.4:13731950-13731969 None:intergenic 20.0%
!! TTGAAAATCATAAAAGAATG+AGG - chr1.4:13731951-13731970 None:intergenic 20.0%
!!! AAAATTTGCTTAAAACCAAA+AGG + chr1.4:13731858-13731877 MS.gene32353:CDS 20.0%
!!! AATAACAAATATATTTTGGG+CGG + chr1.4:13731988-13732007 MS.gene32353:CDS 20.0%
!!! TGGTTTTAAGCAAATTTTTA+AGG - chr1.4:13731856-13731875 None:intergenic 20.0%
! ATATATTGATGCAAATCTCT+TGG - chr1.4:13731785-13731804 None:intergenic 25.0%
! CAATTAAGCCAATTAAACAA+TGG + chr1.4:13733063-13733082 MS.gene32353:CDS 25.0%
! CTAAAAGACGTTATAAATCT+TGG + chr1.4:13732823-13732842 MS.gene32353:CDS 25.0%
! GATAAAAGAAAAATACGTAC+CGG - chr1.4:13732960-13732979 None:intergenic 25.0%
! TACAGTAAAAGAATTGGATT+TGG + chr1.4:13731434-13731453 MS.gene32353:CDS 25.0%
! TATGTTGTCCAAAAAATATC+CGG + chr1.4:13732680-13732699 MS.gene32353:CDS 25.0%
! TTGTTGGACTTAATAATCAT+AGG + chr1.4:13732107-13732126 MS.gene32353:CDS 25.0%
!! TTGTTTGAAATATGTCATCA+AGG - chr1.4:13731915-13731934 None:intergenic 25.0%
!!! ATAGGCAAACAATCATTTTT+GGG + chr1.4:13732125-13732144 MS.gene32353:CDS 25.0%
!!! CAAGAAACTTTTTCTATAGA+TGG - chr1.4:13732354-13732373 None:intergenic 25.0%
!!! TAAGCAAATTTTTAAGGTCT+TGG - chr1.4:13731850-13731869 None:intergenic 25.0%
AAATAAGGATTATCGACCTT+TGG + chr1.4:13732078-13732097 MS.gene32353:CDS 30.0%
AATAAGGATTATCGACCTTT+GGG + chr1.4:13732079-13732098 MS.gene32353:CDS 30.0%
AATGTTCATCATGAGAAAGA+AGG + chr1.4:13731338-13731357 MS.gene32353:CDS 30.0%
ACAAGCATCAAAAATTGTCT+TGG + chr1.4:13731739-13731758 MS.gene32353:CDS 30.0%
CAAGCATCAAAAATTGTCTT+GGG + chr1.4:13731740-13731759 MS.gene32353:CDS 30.0%
CAATATATATCTAAGCAAGC+TGG + chr1.4:13731797-13731816 MS.gene32353:CDS 30.0%
CTCAGAAAAAGAAGCTAAAA+AGG + chr1.4:13732878-13732897 MS.gene32353:CDS 30.0%
GCACAGATGATTAATGATTA+TGG + chr1.4:13732013-13732032 MS.gene32353:CDS 30.0%
GCTTGAAAAAGAAGCTAAAA+AGG + chr1.4:13732845-13732864 MS.gene32353:CDS 30.0%
GGAAATAAAAGCAGAATAGA+TGG + chr1.4:13731473-13731492 MS.gene32353:CDS 30.0%
GTTATGCCAAAAACATTTCA+TGG + chr1.4:13732283-13732302 MS.gene32353:CDS 30.0%
GTTGAGTTCCATTGTTTAAT+TGG - chr1.4:13733074-13733093 None:intergenic 30.0%
TCAAATAACCACTGAAAAGA+AGG - chr1.4:13732178-13732197 None:intergenic 30.0%
TCGAAAGATAGGGAAATATA+AGG + chr1.4:13731628-13731647 MS.gene32353:CDS 30.0%
TGGTTTAGTACAATTGTTAC+CGG + chr1.4:13732938-13732957 MS.gene32353:CDS 30.0%
TTCATCATTCGCAATTGATA+TGG - chr1.4:13732736-13732755 None:intergenic 30.0%
! AAACAATTCCTTCTTTTCAG+TGG + chr1.4:13732167-13732186 MS.gene32353:CDS 30.0%
! ATCCTTATTTGTCTTGTAAG+TGG - chr1.4:13732068-13732087 None:intergenic 30.0%
! CACAATCCATGAAATGTTTT+TGG - chr1.4:13732292-13732311 None:intergenic 30.0%
! CATAGGCAAACAATCATTTT+TGG + chr1.4:13732124-13732143 MS.gene32353:CDS 30.0%
! CATATCGTTAACTATTTTGC+TGG - chr1.4:13732664-13732683 None:intergenic 30.0%
! CATGATGAACATTTTGTTCA+TGG - chr1.4:13731331-13731350 None:intergenic 30.0%
! TGATCTGTTAGAATGGTTTT+AGG - chr1.4:13732232-13732251 None:intergenic 30.0%
!! GAAACTTTTCTGAAAGCAAT+GGG + chr1.4:13732196-13732215 MS.gene32353:CDS 30.0%
!! TAGGCAAACAATCATTTTTG+GGG + chr1.4:13732126-13732145 MS.gene32353:CDS 30.0%
!! TGAAACTTTTCTGAAAGCAA+TGG + chr1.4:13732195-13732214 MS.gene32353:CDS 30.0%
AAAAAGCCCATTTCATGAAG+CGG + chr1.4:13732544-13732563 MS.gene32353:CDS 35.0%
AACACTACAGACTAAAACGA+TGG + chr1.4:13732460-13732479 MS.gene32353:CDS 35.0%
AAGATTCACATGCTTCAAAG+AGG + chr1.4:13731511-13731530 MS.gene32353:CDS 35.0%
AATGAAGTTTGAATCTGAGG+CGG + chr1.4:13731388-13731407 MS.gene32353:CDS 35.0%
AGATTCACATGCTTCAAAGA+GGG + chr1.4:13731512-13731531 MS.gene32353:CDS 35.0%
AGGAAGCAATCACTAAAGTA+TGG + chr1.4:13731878-13731897 MS.gene32353:CDS 35.0%
ATATATCTAAGCAAGCTGGT+GGG + chr1.4:13731801-13731820 MS.gene32353:CDS 35.0%
ATCATATGAACATACTCAGG+TGG - chr1.4:13731692-13731711 None:intergenic 35.0%
ATCTCACTTGATCGAAAGAT+AGG + chr1.4:13731617-13731636 MS.gene32353:CDS 35.0%
ATTGTACCGCTTCATGAAAT+GGG - chr1.4:13732553-13732572 None:intergenic 35.0%
CACCACTTACAAGACAAATA+AGG + chr1.4:13732063-13732082 MS.gene32353:CDS 35.0%
CGAATCATATGAACATACTC+AGG - chr1.4:13731695-13731714 None:intergenic 35.0%
GAAATAATCAAGGTTGGAGT+GGG + chr1.4:13732502-13732521 MS.gene32353:CDS 35.0%
TATATATCTAAGCAAGCTGG+TGG + chr1.4:13731800-13731819 MS.gene32353:CDS 35.0%
TCTCACTTGATCGAAAGATA+GGG + chr1.4:13731618-13731637 MS.gene32353:CDS 35.0%
TGCATCTTGATCTGTTAGAA+TGG - chr1.4:13732239-13732258 None:intergenic 35.0%
TTGGTCAATTTAAGTAGCTG+AGG - chr1.4:13732585-13732604 None:intergenic 35.0%
TTGTCCAAAAAATATCCGGA+TGG + chr1.4:13732684-13732703 MS.gene32353:CDS 35.0%
TTTGGTATTCGTCGAGAATA+TGG + chr1.4:13731452-13731471 MS.gene32353:CDS 35.0%
! CTTTAGTGATTGCTTCCTTT+TGG - chr1.4:13731876-13731895 None:intergenic 35.0%
! TCCAGATTGTGTCGATAAAT+GGG + chr1.4:13731295-13731314 MS.gene32353:CDS 35.0%
! TGTCGAAAATTTGAGACACA+TGG + chr1.4:13732378-13732397 MS.gene32353:intron 35.0%
! TTCCAGATTGTGTCGATAAA+TGG + chr1.4:13731294-13731313 MS.gene32353:CDS 35.0%
!! AAAGAAGGTATTGCCAATAG+TGG + chr1.4:13731353-13731372 MS.gene32353:CDS 35.0%
!! ATGAGATTCTGAAGCTCTAT+TGG - chr1.4:13732604-13732623 None:intergenic 35.0%
!! TCGTTTTAGTCTGTAGTGTT+CGG - chr1.4:13732460-13732479 None:intergenic 35.0%
!!! TAACGTCTTTTAGTCTTACG+TGG - chr1.4:13732816-13732835 None:intergenic 35.0%
!!! TTCTCCATCCGGATATTTTT+TGG - chr1.4:13732691-13732710 None:intergenic 35.0%
AACAGATCAAGATGCAGCAA+TGG + chr1.4:13732243-13732262 MS.gene32353:CDS 40.0%
ACCCATTTATCGACACAATC+TGG - chr1.4:13731299-13731318 None:intergenic 40.0%
ACTAAAACGATGGACAAGAC+AGG + chr1.4:13732470-13732489 MS.gene32353:CDS 40.0%
AGTCCAACAAATACACCCAA+AGG - chr1.4:13732097-13732116 None:intergenic 40.0%
ATTCCACTTGATTCTCCATC+CGG - chr1.4:13732702-13732721 None:intergenic 40.0%
ATTGTCTTGGGAGATGAATC+TGG + chr1.4:13731752-13731771 MS.gene32353:CDS 40.0%
CATACTCAGGTGGAATAAGA+AGG - chr1.4:13731682-13731701 None:intergenic 40.0%
CATTGTACCGCTTCATGAAA+TGG - chr1.4:13732554-13732573 None:intergenic 40.0%
GACAACAACAAAGACACTGT+TGG + chr1.4:13732759-13732778 MS.gene32353:CDS 40.0%
GCAAGGTTGGGAAATAATCA+AGG + chr1.4:13732492-13732511 MS.gene32353:CDS 40.0%
GCTGGACTCTTCATAAACTT+TGG - chr1.4:13732646-13732665 None:intergenic 40.0%
GGAAATAATCAAGGTTGGAG+TGG + chr1.4:13732501-13732520 MS.gene32353:CDS 40.0%
GGGAATGAAGTTTGAATCTG+AGG + chr1.4:13731385-13731404 MS.gene32353:CDS 40.0%
GGTTGGGAAATAATCAAGGT+TGG + chr1.4:13732496-13732515 MS.gene32353:CDS 40.0%
TATATCTAAGCAAGCTGGTG+GGG + chr1.4:13731802-13731821 MS.gene32353:CDS 40.0%
TCAAACTTCATTCCCAAGTG+AGG - chr1.4:13731380-13731399 None:intergenic 40.0%
! CATTTCATGGATTGTGCACT+TGG + chr1.4:13732296-13732315 MS.gene32353:intron 40.0%
! TCTAGAGCAGAAACAAGAAC+AGG + chr1.4:13731575-13731594 MS.gene32353:CDS 40.0%
! TGCCTTAGATATGTGTCGAT+GGG - chr1.4:13731720-13731739 None:intergenic 40.0%
!! ACTTTTCTGAAAGCAATGGG+TGG + chr1.4:13732199-13732218 MS.gene32353:CDS 40.0%
!! CGTTTTAGTCTGTAGTGTTC+GGG - chr1.4:13732459-13732478 None:intergenic 40.0%
!! GCTTCATGAAATGGGCTTTT+TGG - chr1.4:13732545-13732564 None:intergenic 40.0%
ACTCAGGTGGAATAAGAAGG+TGG - chr1.4:13731679-13731698 None:intergenic 45.0%
ATAATCAAGGTTGGAGTGGG+AGG + chr1.4:13732505-13732524 MS.gene32353:CDS 45.0%
CGACCTTTGGGTGTATTTGT+TGG + chr1.4:13732091-13732110 MS.gene32353:CDS 45.0%
CTCCCATCGACACATATCTA+AGG + chr1.4:13731715-13731734 MS.gene32353:CDS 45.0%
GGATGGAGAATCAAGTGGAA+TGG + chr1.4:13732701-13732720 MS.gene32353:CDS 45.0%
GTGCCTTAGATATGTGTCGA+TGG - chr1.4:13731721-13731740 None:intergenic 45.0%
TATCCGGATGGAGAATCAAG+TGG + chr1.4:13732696-13732715 MS.gene32353:CDS 45.0%
TGACAAAAGACGTCAACCGA+CGG + chr1.4:13731547-13731566 MS.gene32353:CDS 45.0%
! GGCAAAAGGCATAAAGACGA+AGG + chr1.4:13732785-13732804 MS.gene32353:CDS 45.0%
AACGATGGACAAGACAGGCA+AGG + chr1.4:13732475-13732494 MS.gene32353:CDS 50.0%
ATGGACAAGACAGGCAAGGT+TGG + chr1.4:13732479-13732498 MS.gene32353:CDS 50.0%
CAACAAAGACACTGTTGGCG+AGG + chr1.4:13732764-13732783 MS.gene32353:CDS 50.0%
TCCCAAGTGAGGTCCACTAT+TGG - chr1.4:13731369-13731388 None:intergenic 50.0%
TGGACAAGACAGGCAAGGTT+GGG + chr1.4:13732480-13732499 MS.gene32353:CDS 50.0%
!! GCCAATAGTGGACCTCACTT+GGG + chr1.4:13731365-13731384 MS.gene32353:CDS 50.0%
!! TGCCAATAGTGGACCTCACT+TGG + chr1.4:13731364-13731383 MS.gene32353:CDS 50.0%
AACAGGATGCACAGCGCGTA+TGG + chr1.4:13731592-13731611 MS.gene32353:CDS 55.0%
CAAAAGACGTCAACCGACGG+CGG + chr1.4:13731550-13731569 MS.gene32353:CDS 55.0%
GACACTGTTGGCGAGGCAAA+AGG + chr1.4:13732771-13732790 MS.gene32353:CDS 55.0%
GCTGGTGGGGTAGAGATTGT+CGG + chr1.4:13731815-13731834 MS.gene32353:CDS 55.0%
! TCTGCTCTAGACTCCGCCGT+CGG - chr1.4:13731566-13731585 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 13731278 13733143 13731278 ID=MS.gene32353
chr1.4 mRNA 13731278 13733143 13731278 ID=MS.gene32353.t1;Parent=MS.gene32353
chr1.4 exon 13731278 13732308 13731278 ID=MS.gene32353.t1.exon1;Parent=MS.gene32353.t1
chr1.4 CDS 13731278 13732308 13731278 ID=cds.MS.gene32353.t1;Parent=MS.gene32353.t1
chr1.4 exon 13732413 13732959 13732413 ID=MS.gene32353.t1.exon2;Parent=MS.gene32353.t1
chr1.4 CDS 13732413 13732959 13732413 ID=cds.MS.gene32353.t1;Parent=MS.gene32353.t1
chr1.4 exon 13733036 13733143 13733036 ID=MS.gene32353.t1.exon3;Parent=MS.gene32353.t1
chr1.4 CDS 13733036 13733143 13733036 ID=cds.MS.gene32353.t1;Parent=MS.gene32353.t1
Gene Sequence

>MS.gene32353

ATGGATTCATCGTCAATTCCAGATTGTGTCGATAAATGGGTGAACAATATCCATGAACAAAATGTTCATCATGAGAAAGAAGGTATTGCCAATAGTGGACCTCACTTGGGAATGAAGTTTGAATCTGAGGCGGCGACATATGAATTTTATAATGATTACAGTAAAAGAATTGGATTTGGTATTCGTCGAGAATATGGAAATAAAAGCAGAATAGATGGAGTTTTGACTTCGAGAAGATTCACATGCTTCAAAGAGGGTACGCTAGTTGTTGACAAAAGACGTCAACCGACGGCGGAGTCTAGAGCAGAAACAAGAACAGGATGCACAGCGCGTATGGTTATCTCACTTGATCGAAAGATAGGGAAATATAAGGTTGTTGACTTTGAAGCTCAACATAACCACCTTCTTATTCCACCTGAGTATGTTCATATGATTCGCTCCCATCGACACATATCTAAGGCACAAGCATCAAAAATTGTCTTGGGAGATGAATCTGGCTTAAGACCAAGAGATTTGCATCAATATATATCTAAGCAAGCTGGTGGGGTAGAGATTGTCGGTTTTACAAGCCAAGACCTTAAAAATTTGCTTAAAACCAAAAGGAAGCAATCACTAAAGTATGGTGAAGTGAGTGCCTTGATGACATATTTCAAACAAGAAAGTGAAAATCCCTCATTCTTTTATGATTTTCAAATGGATGTTGATGAGCTAATAACAAATATATTTTGGGCGGATGCACAGATGATTAATGATTATGGATACTTTGGTGATATCATCACTTTTGACACCACTTACAAGACAAATAAGGATTATCGACCTTTGGGTGTATTTGTTGGACTTAATAATCATAGGCAAACAATCATTTTTGGGGCAACAATGTTATATGATGAAACAATTCCTTCTTTTCAGTGGTTATTTGAAACTTTTCTGAAAGCAATGGGTGGAGAAAAGCCTAAAACCATTCTAACAGATCAAGATGCAGCAATGGCTAAAACTATTTCTTTAGTTATGCCAAAAACATTTCATGGATTGTGCACTTGGCATATAAGACAGAACGCTCTTAAGCATGTGAACCATCTATAGAAAAAGTTTCTTGTGATTGTCGAAAATTTGAGACACATGGCATCTTATGTAGTCATGCTTTGAAAGTTTTAGATGTCATGAACATTAAGTTAATTCCCGAACACTACAGACTAAAACGATGGACAAGACAGGCAAGGTTGGGAAATAATCAAGGTTGGAGTGGGAGGCATGTTGAATTAGACCAAAAAGCCCATTTCATGAAGCGGTACAATGAGTTATGTCCTCAGCTACTTAAATTGACCAATAGAGCTTCAGAATCTCATGAAACTTACACATTTTTGTCCAAAGTTTATGAAGAGTCCAGCAAAATAGTTAACGATATGTTGTCCAAAAAATATCCGGATGGAGAATCAAGTGGAATGGTTCATGTATCCATATCAATTGCGAATGATGAAGTCGACAACAACAAAGACACTGTTGGCGAGGCAAAAGGCATAAAGACGAAGGATTGTCCACGTAAGACTAAAAGACGTTATAAATCTTGGCTTGAAAAAGAAGCTAAAAAGGCAAAGATGCACTCAGAAAAAGAAGCTAAAAAGGCAAAGATGCACTCAGAAAAATTGAAAAAATCTCAAGATGGTTTAGTACAATTGTTACCGGTACGTATTTTTCTTTTATCAATTTGTTATATTTTGTTTTACCTATCATTTATTTAACTTTTCTTGTATTATGCAGCAATCAAGCAACACAATTCAAGCGACACAATTAAGCCAATTAAACAATGGAACTCAACATCAAGATACAATTCAAGCAACACAATCAAGCCAAGTTAACTCATTGTAA
Protein sequence

>MS.gene32353.t1

MDSSSIPDCVDKWVNNIHEQNVHHEKEGIANSGPHLGMKFESEAATYEFYNDYSKRIGFGIRREYGNKSRIDGVLTSRRFTCFKEGTLVVDKRRQPTAESRAETRTGCTARMVISLDRKIGKYKVVDFEAQHNHLLIPPEYVHMIRSHRHISKAQASKIVLGDESGLRPRDLHQYISKQAGGVEIVGFTSQDLKNLLKTKRKQSLKYGEVSALMTYFKQESENPSFFYDFQMDVDELITNIFWADAQMINDYGYFGDIITFDTTYKTNKDYRPLGVFVGLNNHRQTIIFGATMLYDETIPSFQWLFETFLKAMGGEKPKTILTDQDAAMAKTISLVMPKTFHGFHALKVLDVMNIKLIPEHYRLKRWTRQARLGNNQGWSGRHVELDQKAHFMKRYNELCPQLLKLTNRASESHETYTFLSKVYEESSKIVNDMLSKKYPDGESSGMVHVSISIANDEVDNNKDTVGEAKGIKTKDCPRKTKRRYKSWLEKEAKKAKMHSEKEAKKAKMHSEKLKKSQDGLVQLLPQSSNTIQATQLSQLNNGTQHQDTIQATQSSQVNSL