AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene32378

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32378.t1 MTR_0042s0130 35.573 253 158 4 118 369 81 329 5.22e-40 151
MS.gene32378.t1 MTR_2g018005 36.683 199 117 4 87 285 40 229 1.26e-30 120
MS.gene32378.t1 MTR_6g009010 32.759 232 130 6 93 323 44 250 9.36e-30 119
MS.gene32378.t1 MTR_1g100687 31.776 214 136 5 155 363 14 222 7.56e-29 115
MS.gene32378.t1 MTR_1g051330 32.035 231 151 4 134 363 213 438 1.09e-28 123
MS.gene32378.t1 MTR_7g027090 57.471 87 36 1 304 390 41 126 1.17e-24 102
MS.gene32378.t1 MTR_7g027090 37.500 152 70 6 191 333 1 136 1.03e-15 76.3
MS.gene32378.t1 MTR_2g055450 28.685 251 161 4 119 369 99 331 2.19e-24 107
MS.gene32378.t1 MTR_6g034215 27.241 290 180 8 100 385 50 312 3.37e-24 104
MS.gene32378.t1 MTR_0402s0040 29.096 354 183 11 398 738 93 391 6.35e-24 106
MS.gene32378.t1 MTR_4g020630 23.111 450 301 14 134 575 2 414 7.71e-23 103
MS.gene32378.t1 MTR_2g078880 32.275 189 107 4 95 283 22 189 8.46e-23 97.8
MS.gene32378.t1 MTR_1g051860 51.163 86 42 0 295 380 2 87 2.07e-22 92.4
MS.gene32378.t1 MTR_6g046587 33.113 151 99 2 130 280 104 252 1.74e-18 87.0
MS.gene32378.t1 MTR_8g470880 33.735 166 103 3 145 308 363 523 4.01e-18 89.0
MS.gene32378.t1 MTR_8g463110 30.052 193 128 4 197 385 119 308 4.27e-18 89.4
MS.gene32378.t1 MTR_6g034715 30.075 133 92 1 154 286 5 136 5.48e-17 79.7
MS.gene32378.t1 MTR_4g044433 34.247 146 93 3 89 234 7 149 1.78e-16 77.8
MS.gene32378.t1 MTR_8g442690 26.866 268 178 6 137 393 29 289 2.25e-15 80.1
MS.gene32378.t1 MTR_3g436680 33.333 147 90 3 228 368 2 146 3.25e-14 77.0
MS.gene32378.t1 MTR_1g054775 25.957 235 166 6 119 351 616 844 2.02e-13 75.1
MS.gene32378.t1 MTR_3g044860 28.889 180 121 4 190 368 4 177 4.35e-13 71.2
MS.gene32378.t1 MTR_0114s0080 36.036 111 70 1 169 279 15 124 7.22e-13 67.0
MS.gene32378.t1 MTR_0111s0070 33.526 173 100 8 187 351 253 418 1.23e-11 68.6
MS.gene32378.t1 MTR_1g008290 31.967 122 77 3 387 506 5 122 1.47e-11 63.5
MS.gene32378.t1 MTR_0044s0090 28.177 181 116 5 223 393 6 182 6.27e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32378.t1 AT4G38180 31.638 177 120 1 504 679 465 641 3.53e-23 106
MS.gene32378.t1 AT4G38180 33.333 114 76 0 389 502 134 247 5.82e-13 73.2
MS.gene32378.t1 AT3G22170 27.803 223 145 3 498 715 444 655 2.93e-18 90.5
MS.gene32378.t1 AT3G22170 27.803 223 145 3 498 715 444 655 2.93e-18 90.5
MS.gene32378.t1 AT4G15090 27.273 253 169 4 498 745 435 677 5.38e-18 89.7
MS.gene32378.t1 AT4G15090 27.273 253 169 4 498 745 435 677 5.38e-18 89.7
MS.gene32378.t1 AT4G15090 27.273 253 169 4 498 745 435 677 5.38e-18 89.7
MS.gene32378.t1 AT4G15090 27.273 253 169 4 498 745 435 677 5.38e-18 89.7
MS.gene32378.t1 AT4G15090 27.273 253 169 4 498 745 435 677 5.38e-18 89.7
MS.gene32378.t1 AT4G38170 25.749 167 123 1 507 672 233 399 1.45e-16 84.3
MS.gene32378.t1 AT3G06250 29.268 164 115 1 531 693 562 725 2.17e-15 80.9
MS.gene32378.t1 AT3G06250 29.268 164 115 1 531 693 562 725 2.17e-15 80.9
MS.gene32378.t1 AT3G06250 29.268 164 115 1 531 693 562 725 2.17e-15 80.9
MS.gene32378.t1 AT2G27110 26.866 201 143 2 532 731 438 635 2.55e-15 80.9
MS.gene32378.t1 AT2G27110 26.866 201 143 2 532 731 438 635 2.55e-15 80.9
MS.gene32378.t1 AT2G27110 26.866 201 143 2 532 731 293 490 3.40e-15 80.5
MS.gene32378.t1 AT5G18960 27.322 183 128 2 516 693 567 749 7.84e-15 79.3
MS.gene32378.t1 AT5G18960 27.322 183 128 2 516 693 567 749 7.84e-15 79.3
MS.gene32378.t1 AT4G19990 27.273 297 188 5 498 777 383 668 3.09e-14 77.4
MS.gene32378.t1 AT4G19990 27.273 297 188 5 498 777 383 668 3.09e-14 77.4
MS.gene32378.t1 AT4G19990 27.273 297 188 5 498 777 383 668 3.84e-14 77.0
MS.gene32378.t1 AT4G19990 27.273 297 188 5 498 777 383 668 3.84e-14 77.0
MS.gene32378.t1 AT4G19990 27.273 297 188 5 498 777 383 668 4.04e-14 77.0
MS.gene32378.t1 AT4G19990 27.273 297 188 5 498 777 326 611 4.12e-14 77.0
MS.gene32378.t1 AT4G19990 27.273 297 188 5 498 777 397 682 4.24e-14 77.0
MS.gene32378.t1 AT1G76320 23.050 282 205 5 498 774 372 646 7.88e-14 75.9
MS.gene32378.t1 AT1G76320 23.050 282 205 5 498 774 372 646 8.38e-14 75.9
MS.gene32378.t1 AT1G76320 23.050 282 205 5 498 774 372 646 8.38e-14 75.9
MS.gene32378.t1 AT1G76320 23.239 284 202 6 498 774 372 646 1.28e-13 75.1
MS.gene32378.t1 AT2G32250 25.561 223 161 2 504 726 385 602 3.19e-12 70.9
MS.gene32378.t1 AT2G32250 25.561 223 161 2 504 726 385 602 3.19e-12 70.9
MS.gene32378.t1 AT2G32250 25.561 223 161 2 504 726 385 602 3.19e-12 70.9
MS.gene32378.t1 AT2G32250 25.561 223 161 2 504 726 385 602 3.19e-12 70.9
MS.gene32378.t1 AT2G32250 25.561 223 161 2 504 726 385 602 3.19e-12 70.9
MS.gene32378.t1 AT2G32250 25.561 223 161 2 504 726 344 561 3.31e-12 70.9
MS.gene32378.t1 AT2G32250 25.561 223 161 2 504 726 344 561 3.68e-12 70.5
MS.gene32378.t1 AT2G32250 25.561 223 161 2 504 726 385 602 4.87e-12 70.1

Find 150 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATATGATTTCAATGTTTC+GGG 0.187917 1.4:-13296264 MS.gene32378:CDS
CAATTAAGTGAGAGCTTTAA+TGG 0.201468 1.4:-13291566 MS.gene32378:CDS
CATCTCTTAAGGATGTATTT+TGG 0.227711 1.4:+13291116 None:intergenic
CTCATATGATTTCAATGTTT+CGG 0.241565 1.4:-13296265 MS.gene32378:CDS
GCTACGTGATTGTGGAATTT+TGG 0.255265 1.4:-13295973 MS.gene32378:CDS
AGAGCTTTAATGGCCGTTTA+AGG 0.266195 1.4:-13291556 MS.gene32378:CDS
ACATCCACTTCGAGCATTCT+TGG 0.267623 1.4:+13291091 None:intergenic
GCAATCATTTAAATTCTAAA+AGG 0.268916 1.4:-13292276 MS.gene32378:CDS
ACGACATGGTTATAAATATA+GGG 0.275234 1.4:-13297770 None:intergenic
CTTCAATGCATCTGAAGTTA+TGG 0.286009 1.4:-13290790 MS.gene32378:CDS
TGGAGACTTACTGGTGTATA+TGG 0.291355 1.4:-13296434 MS.gene32378:CDS
CATCCATCTTGTGAGGTTCT+TGG 0.298227 1.4:-13297732 MS.gene32378:CDS
GTTGTTGCTGTTAGTTGTTC+AGG 0.308904 1.4:-13296581 MS.gene32378:CDS
GAATACGATGTATGCTTGTT+TGG 0.309520 1.4:-13291278 MS.gene32378:CDS
TTGAAAACATCTCGAGTAAA+AGG 0.311854 1.4:-13290902 MS.gene32378:CDS
GGGTTGCAAGTTCATGGTTA+TGG 0.320894 1.4:+13292464 None:intergenic
TGTTCAGCGCATTTGGTCAT+TGG 0.324780 1.4:-13292532 MS.gene32378:CDS
TACGACATGGTTATAAATAT+AGG 0.326116 1.4:-13297771 None:intergenic
GAGAAGGAAGGTGGGAATTT+CGG 0.328742 1.4:-13296293 MS.gene32378:CDS
CTGGCGGTCTTGCAATGTTT+TGG 0.330724 1.4:-13296541 MS.gene32378:CDS
ATCCATCTTGTGAGGTTCTT+GGG 0.331730 1.4:-13297731 MS.gene32378:CDS
CTTCAGATCATCTACCTTTG+TGG 0.334014 1.4:-13296067 MS.gene32378:CDS
AACTGGTTGTTCAGCGCATT+TGG 0.336400 1.4:-13292539 MS.gene32378:CDS
CGGAATGTTCAAGGTTTATC+TGG 0.341008 1.4:-13296605 MS.gene32378:CDS
GCTAGATGATATGCTATATA+AGG 0.342493 1.4:-13291477 MS.gene32378:CDS
ATCATCTACCTTTGTGGTTG+CGG 0.343655 1.4:-13296061 MS.gene32378:CDS
GATGAGAAATGGAGACTTAC+TGG 0.344167 1.4:-13296443 MS.gene32378:CDS
TGGACGCAGTGAAAGGTTCT+TGG 0.345822 1.4:-13295953 MS.gene32378:CDS
TATTCAATGATGGCATCTTC+TGG 0.352708 1.4:+13291429 None:intergenic
CATTCTTGGTCCATCTCTTA+AGG 0.360739 1.4:+13291105 None:intergenic
AAAATCATGTGATCTTGTTT+TGG 0.367449 1.4:-13291330 MS.gene32378:CDS
CAAGACATCAATATGGTTAG+AGG 0.367621 1.4:+13296484 None:intergenic
TCAAATTCCATACCAACATT+TGG 0.374980 1.4:+13297509 None:intergenic
AATACGATGTATGCTTGTTT+GGG 0.376467 1.4:-13291277 MS.gene32378:CDS
AATTACAACCTTTCTGACTT+AGG 0.383221 1.4:-13296230 MS.gene32378:CDS
AACTGGAGTAAGGATCGTTT+TGG 0.385854 1.4:-13295873 MS.gene32378:CDS
AATATGTACGCAGGCTTTGT+TGG 0.387200 1.4:-13297458 MS.gene32378:CDS
CTATTCGAGTACTTAATCAT+TGG 0.395437 1.4:-13291157 MS.gene32378:CDS
ACTTTGATCTTCAGAGTTGA+AGG 0.396762 1.4:+13291232 None:intergenic
ATATCAAGGCACGCCTTGAT+AGG 0.397106 1.4:-13296157 MS.gene32378:CDS
AGTTGTCTTAGATCGCTCTA+AGG 0.405571 1.4:-13291024 MS.gene32378:CDS
TTCAATGCATCTGAAGTTAT+GGG 0.414721 1.4:-13290789 MS.gene32378:CDS
CTGGTCCTCATTCTATCCTT+CGG 0.419416 1.4:-13296117 MS.gene32378:CDS
GTGTGAATGATCTTTCTAAC+TGG 0.423780 1.4:-13295890 MS.gene32378:CDS
TTTCAATCTGATTCTTTCAC+AGG 0.425515 1.4:-13296318 MS.gene32378:CDS
TTCTAAAAGGATGAAGGAAA+TGG 0.429410 1.4:-13292263 MS.gene32378:CDS
GGATGTGTTCTTGACAATAA+AGG 0.432868 1.4:-13291074 MS.gene32378:CDS
AGGTTCTTGGGAGGTGGCAG+GGG 0.433092 1.4:-13295940 MS.gene32378:CDS
AAAGGTTCTTGGGAGGTGGC+AGG 0.437714 1.4:-13295942 MS.gene32378:CDS
GGACGCAGTGAAAGGTTCTT+GGG 0.440298 1.4:-13295952 MS.gene32378:CDS
AATAAGTTTCGGAATGTTCA+AGG 0.442621 1.4:-13296614 MS.gene32378:CDS
TGGTAACTTATGCCTACTCC+TGG 0.442738 1.4:+13292441 None:intergenic
TCAAGCTACTGAAGTTGAAA+TGG 0.444267 1.4:-13292395 MS.gene32378:CDS
CCTGCCACCTCGGATTCTAA+TGG 0.444857 1.4:-13297672 MS.gene32378:intron
TTTAAATTCTAAAAGGATGA+AGG 0.445058 1.4:-13292269 MS.gene32378:CDS
TAGCAATCAAGACATCAATA+TGG 0.445453 1.4:+13296477 None:intergenic
ACCATGATCTGCACTCTGTA+TGG 0.447129 1.4:+13290875 None:intergenic
TGTGGTTGCGGCTTGCATCT+AGG 0.448072 1.4:-13296049 MS.gene32378:CDS
GTTGAAATGGCGAACCGGTC+CGG 0.468344 1.4:-13292382 MS.gene32378:CDS
TTGAGAACGGAGATGAGAAA+TGG 0.470528 1.4:-13296454 MS.gene32378:CDS
TACAGATAAGTGGCTTCTCA+TGG 0.472957 1.4:-13296345 MS.gene32378:CDS
AATTGATCAGTTAGCATTGG+TGG 0.483141 1.4:-13296372 MS.gene32378:CDS
GCTTCATGTAAAGCCTTAAA+CGG 0.485722 1.4:+13291543 None:intergenic
TGTCAAGATACACTAGCAAT+TGG 0.487500 1.4:-13290560 MS.gene32378:CDS
AATTAGTTTCTGACGTATGC+CGG 0.490277 1.4:+13292363 None:intergenic
CTGAAGTTGAAATGGCGAAC+CGG 0.490381 1.4:-13292387 MS.gene32378:CDS
TGATCTTTCTAACTGGAGTA+AGG 0.490843 1.4:-13295883 MS.gene32378:CDS
GTTTCTGACGTATGCCGGAC+CGG 0.493182 1.4:+13292368 None:intergenic
TACCGTAGCAATATCATGTA+AGG 0.494189 1.4:-13290988 MS.gene32378:CDS
GATGGTGTATCCAGAGGAAA+AGG 0.496843 1.4:-13290753 MS.gene32378:intron
CATTGAAAATGATGTAACCT+TGG 0.502984 1.4:-13297543 MS.gene32378:CDS
GGTAACTTATGCCTACTCCT+GGG 0.504717 1.4:+13292442 None:intergenic
TTGAAGGTGATAAATTTACT+TGG 0.504784 1.4:-13296205 MS.gene32378:CDS
CGATCTAAGACAACTAACTT+AGG 0.508239 1.4:+13291032 None:intergenic
CTCGAATAGCATGGCAGCAA+AGG 0.509751 1.4:+13291171 None:intergenic
GCTGTTAGTTGTTCAGGAGA+AGG 0.513956 1.4:-13296575 MS.gene32378:CDS
GACTAGATTAAATGACTTGG+TGG 0.514288 1.4:-13295751 MS.gene32378:CDS
AAGGTTCTTGGGAGGTGGCA+GGG 0.515607 1.4:-13295941 MS.gene32378:CDS
TATAGTTCCAAATGTTGGTA+TGG 0.518391 1.4:-13297516 MS.gene32378:CDS
GAAGGAAGGAGTTGAAGTAG+AGG 0.520875 1.4:-13296519 MS.gene32378:CDS
GTAACTTATGCCTACTCCTG+GGG 0.521725 1.4:+13292443 None:intergenic
GTTAATTGATCAGTTAGCAT+TGG 0.522162 1.4:-13296375 MS.gene32378:CDS
GACGATATAGTTCCAAATGT+TGG 0.524084 1.4:-13297521 MS.gene32378:CDS
ACTCGCAAAGATATCAGAAC+TGG 0.524113 1.4:-13292556 MS.gene32378:CDS
CTTCAAAACCTAAGTCAGAA+AGG 0.524728 1.4:+13296222 None:intergenic
CCATTAGAATCCGAGGTGGC+AGG 0.526150 1.4:+13297672 None:intergenic
CGAAGGATAGAATGAGGACC+AGG 0.526233 1.4:+13296118 None:intergenic
CAAGAACCTCACAAGATGGA+TGG 0.528873 1.4:+13297733 None:intergenic
ACTTGGCATAATAGACAAGA+TGG 0.530670 1.4:-13296188 MS.gene32378:CDS
TTCATTAGTCGACAAGTTGG+AGG 0.532160 1.4:-13292334 MS.gene32378:CDS
CAAAATACATCCTTAAGAGA+TGG 0.533868 1.4:-13291115 MS.gene32378:CDS
GGATGATAGCGACGCCAGCC+TGG 0.535289 1.4:-13296136 MS.gene32378:CDS
ATGATGTTCATATTCAATGA+TGG 0.538522 1.4:+13291419 None:intergenic
CTCCCAAGAACCTCACAAGA+TGG 0.539182 1.4:+13297729 None:intergenic
GGAGAAGGTAATAGACGTGC+TGG 0.541136 1.4:-13296560 MS.gene32378:CDS
ACAGATAAGTGGCTTCTCAT+GGG 0.541180 1.4:-13296344 MS.gene32378:CDS
TTATGCTTCATATGAGACTC+CGG 0.547336 1.4:+13296407 None:intergenic
TATGCTTGTTTGGGGCTATG+AGG 0.548312 1.4:-13291268 MS.gene32378:CDS
GATTCTTTCACAGGATGAGA+AGG 0.559513 1.4:-13296309 MS.gene32378:CDS
AGTGAAAGGTTCTTGGGAGG+TGG 0.560585 1.4:-13295946 MS.gene32378:CDS
TAACTGATCAATTAACTCGC+AGG 0.561343 1.4:+13296382 None:intergenic
AGATGGTGTGTCTAATATCA+AGG 0.562262 1.4:-13296171 MS.gene32378:CDS
ATTCTATCCTTCGGCTTCAG+TGG 0.564902 1.4:-13296108 MS.gene32378:CDS
AGGATGAAGGAAATGGAGGA+AGG 0.565027 1.4:-13292256 MS.gene32378:intron
AGAATCCAATGGAAAATATG+TGG 0.565466 1.4:-13292506 MS.gene32378:CDS
TCCATACAGAGTGCAGATCA+TGG 0.570905 1.4:-13290876 MS.gene32378:CDS
TTATACAGCTACAACAAGAC+AGG 0.571173 1.4:-13290603 MS.gene32378:CDS
GGATAGAATGAGGACCAGGC+TGG 0.571272 1.4:+13296122 None:intergenic
CAGATAAGTGGCTTCTCATG+GGG 0.571506 1.4:-13296343 MS.gene32378:CDS
AATTCTAACACATTAAAGGT+AGG 0.573003 1.4:+13291521 None:intergenic
TAAAAGGATGAAGGAAATGG+AGG 0.573459 1.4:-13292260 MS.gene32378:CDS
GCGTCGCTATCATCCTATCA+AGG 0.576629 1.4:+13296144 None:intergenic
AATAAGCAAATCCAGCATGA+GGG 0.577261 1.4:-13295801 MS.gene32378:CDS
GAATGTTGGCTACGTGATTG+TGG 0.579436 1.4:-13295981 MS.gene32378:CDS
CGACATGGTTATAAATATAG+GGG 0.582443 1.4:-13297769 None:intergenic
ATTTGGAACTATATCGTCCA+AGG 0.586186 1.4:+13297526 None:intergenic
GAGTTCATTAGTCGACAAGT+TGG 0.587463 1.4:-13292337 MS.gene32378:CDS
TGGTGGAAAGTACAGATAAG+TGG 0.589110 1.4:-13296355 MS.gene32378:CDS
TCACAGGATGAGAAGGAAGG+TGG 0.592911 1.4:-13296302 MS.gene32378:CDS
AAGAACCTCACAAGATGGAT+GGG 0.595753 1.4:+13297734 None:intergenic
GTCATTGGTCGAGAATCCAA+TGG 0.597725 1.4:-13292517 MS.gene32378:CDS
CACAGGATGAGAAGGAAGGT+GGG 0.599669 1.4:-13296301 MS.gene32378:CDS
TCCTACCCATCCATCTTGTG+AGG 0.599686 1.4:-13297739 MS.gene32378:CDS
TAATTGTGTTGTTGTAGCAC+CGG 0.601043 1.4:+13291583 None:intergenic
TGAAGCCGAAGGATAGAATG+AGG 0.602811 1.4:+13296112 None:intergenic
CTTTCACAGGATGAGAAGGA+AGG 0.604149 1.4:-13296305 MS.gene32378:CDS
GTCTTGATTGCTATTGAGAA+CGG 0.605942 1.4:-13296467 MS.gene32378:CDS
AGCCTTACATGATATTGCTA+CGG 0.606303 1.4:+13290986 None:intergenic
TGAGACTAGATTAAATGACT+TGG 0.607795 1.4:-13295754 MS.gene32378:CDS
TCAATTACCATTAGAATCCG+AGG 0.608459 1.4:+13297665 None:intergenic
TCATTAGTCGACAAGTTGGA+GGG 0.610088 1.4:-13292333 MS.gene32378:CDS
TAATAAGCAAATCCAGCATG+AGG 0.610129 1.4:-13295802 MS.gene32378:CDS
GTTGGAGGGAGAGAAAATGT+TGG 0.613113 1.4:-13292319 MS.gene32378:CDS
CAATACAGCTCCTGCCACCT+CGG 0.617505 1.4:-13297682 MS.gene32378:CDS
ACCTCACAAGATGGATGGGT+AGG 0.620207 1.4:+13297738 None:intergenic
TGGACCAAGAATGCTCGAAG+TGG 0.625465 1.4:-13291095 MS.gene32378:CDS
ATAAGCAAATCCAGCATGAG+GGG 0.628277 1.4:-13295800 MS.gene32378:CDS
CGCAGTGAAAGGTTCTTGGG+AGG 0.633772 1.4:-13295949 MS.gene32378:CDS
CCAATTATACAGGTAGAGCA+AGG 0.640562 1.4:-13290632 MS.gene32378:intron
AAATGCACCACTGAAGCCGA+AGG 0.657048 1.4:+13296101 None:intergenic
GATTAAGTACTCGAATAGCA+TGG 0.658504 1.4:+13291162 None:intergenic
AGATGATATGCTATATAAGG+AGG 0.659157 1.4:-13291474 MS.gene32378:CDS
TAACTTATGCCTACTCCTGG+GGG 0.660240 1.4:+13292444 None:intergenic
AAGAAGGATGGTGTATCCAG+AGG 0.663396 1.4:-13290759 MS.gene32378:CDS
ATACGATGTATGCTTGTTTG+GGG 0.666991 1.4:-13291276 MS.gene32378:CDS
GAAGGTAATAGACGTGCTGG+CGG 0.674574 1.4:-13296557 MS.gene32378:CDS
TAAGCAAATCCAGCATGAGG+GGG 0.678445 1.4:-13295799 MS.gene32378:CDS
GATGCAAGCCGCAACCACAA+AGG 0.679518 1.4:+13296053 None:intergenic
AGATAAGTGGCTTCTCATGG+GGG 0.681797 1.4:-13296342 MS.gene32378:CDS
ATTACCATTAGAATCCGAGG+TGG 0.754496 1.4:+13297668 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr1.4 gene 13290554 13297787 13290554 ID=MS.gene32378
chr1.4 mRNA 13290554 13297787 13290554 ID=MS.gene32378.t1;Parent=MS.gene32378
chr1.4 exon 13297673 13297787 13297673 ID=MS.gene32378.t1.exon1;Parent=MS.gene32378.t1
chr1.4 CDS 13297673 13297787 13297673 ID=cds.MS.gene32378.t1;Parent=MS.gene32378.t1
chr1.4 exon 13297426 13297581 13297426 ID=MS.gene32378.t1.exon2;Parent=MS.gene32378.t1
chr1.4 CDS 13297426 13297581 13297426 ID=cds.MS.gene32378.t1;Parent=MS.gene32378.t1
chr1.4 exon 13295748 13296647 13295748 ID=MS.gene32378.t1.exon3;Parent=MS.gene32378.t1
chr1.4 CDS 13295748 13296647 13295748 ID=cds.MS.gene32378.t1;Parent=MS.gene32378.t1
chr1.4 exon 13292257 13292595 13292257 ID=MS.gene32378.t1.exon4;Parent=MS.gene32378.t1
chr1.4 CDS 13292257 13292595 13292257 ID=cds.MS.gene32378.t1;Parent=MS.gene32378.t1
chr1.4 exon 13290754 13291605 13290754 ID=MS.gene32378.t1.exon5;Parent=MS.gene32378.t1
chr1.4 CDS 13290754 13291605 13290754 ID=cds.MS.gene32378.t1;Parent=MS.gene32378.t1
chr1.4 exon 13290554 13290642 13290554 ID=MS.gene32378.t1.exon6;Parent=MS.gene32378.t1
chr1.4 CDS 13290554 13290642 13290554 ID=cds.MS.gene32378.t1;Parent=MS.gene32378.t1
Gene Sequence

>MS.gene32378

ATGGTTATAAATATAGGGGATTTAATTCCTACCCATCCATCTTGTGAGGTTCTTGGGAGCAATGTCAATGAATCAAGAAATTGCAATACAGCTCCTGCCACCTCGGATTCTAATGGTAATTGACAATTTACTGATAGCTTTAATATGTGGTATGTGCTTGTTTATTTTTCCTTTGAATTGGTCTTAATATAGTTTCTCTTTTACAGTGAAATTCAAGGACAACATTGAAAATGATGTAACCTTGGACGATATAGTTCCAAATGTTGGTATGGAATTTGATACTGAAGATCATGCATATAGATTTTATAATATGTACGCAGGCTTTGTTGGTTTTAGTGTTAGAAAAGATTGGAGAAACAAAAGTAAATTAGATAAGGAGATTGTGATATCTCGAAAATTTTCTTGTTTTAAAGAAGGCTTCAAGAGAATAAAAGATGAAGAAGAGCGAAATATTTTTAACAGCCTTTCACTTGGCGATTAGCGATTAGCGGCGATTAGCGGTACTCTCTGCAATTAGGATTACGATTTAACAGCCTTTCAAAGCACCGATAGGTTTCAATCTTTCAACACCAATTAGCCATTAAGCTACAAGTTGTTTTGGTGGCTTTCTACTTCCTTTAGTTGGCAAGTTTCAATCGGTGGATGAATTAGTGAATTATTCACTCTCTGTCTAATTCACCTTGATTCTTGATTTGCATCTCTATGATATGCTAGGTAGTTTTTTCAATCATGATTACTAGGCTCTAAAGAAATTTGTGATGCGATGATTAAACGGTGCCGCTTTTCTTCCGTTCCATTCACGAAGTAATCTGTCTGTCGACGCTGTTATTGTTTCAACATGTCCCCTCTTCATATCAAGTAAGAATACCTTTAACGCTTTTGATGAAGGATTGTTGGTTTTCCCTTTTAATAAGGAGTATGTTGGTTTTCCCTTTTAATAAGGAGTATTGATTTTCCCTTTTAATAAGGAGTATTGAATCCCCTTTCAATAAGGAGTTTTTTGTGTTTTATCGGAGACAATGAGTTTCATAGCTTGGAATTGTCGTGGCCTTGGTAATCTACAGGCAATTAGTGCGTTCAAAAAGCTCTTAAGAGATAAAAATCCCATATGTGTATTCCTCTCAGAAACAAAGAAAAAAGATTTTGAGATGAATAAGTTTCGGAATGTTCAAGGTTTATCTGGTGTTGTTGCTGTTAGTTGTTCAGGAGAAGGTAATAGACGTGCTGGCGGTCTTGCAATGTTTTGGAAGGAAGGAGTTGAAGTAGAGGTATTATCTATTTCCTCTAACCATATTGATGTCTTGATTGCTATTGAGAACGGAGATGAGAAATGGAGACTTACTGGTGTATATGGTTTTCCGGAGTCTCATATGAAGCATAAAACCTGCGAGTTAATTGATCAGTTAGCATTGGTGGAAAGTACAGATAAGTGGCTTCTCATGGGGGATTTCAATCTGATTCTTTCACAGGATGAGAAGGAAGGTGGGAATTTCGGCAACTCTCATATGATTTCAATGTTTCGGGACACTCTTCAAAATTACAACCTTTCTGACTTAGGTTTTGAAGGTGATAAATTTACTTGGCATAATAGACAAGATGGTGTGTCTAATATCAAGGCACGCCTTGATAGGATGATAGCGACGCCAGCCTGGTCCTCATTCTATCCTTCGGCTTCAGTGGTGCATTTAACAAGATATGCTTCAGATCATCTACCTTTGTGGTTGCGGCTTGCATCTAGGAAGCCAAAGAAAAAACAAAATGTTCTTAAATTACAACGTTTTGAAGAATGTTGGCTACGTGATTGTGGAATTTTGGACGCAGTGAAAGGTTCTTGGGAGGTGGCAGGGGATTCTATGTCTGACAAAATTAATCGATGTGTGAATGATCTTTCTAACTGGAGTAAGGATCGTTTTGGTGATGTTCCCAATCAGATAAAAAATGTTCAAAAGAAATTGGATGATCTTAATAAGCAAATCCAGCATGAGGGGGTGATGTCAGAAATTAGACGAGTTGAGACTAGATTAAATGACTTGGTGGAGAGTGAAGAAATTTGGTGGGCCCAGAGGTCTCGTGCTTTGTGGTTAAAGCATGGAGATAAAAATACAAAATTTTTTCATCAAAAAGCTTCTCAGAGAAAATCTAGAAATTGGGTGGAATCTATATGAGACGATCATGGGGATAAATTTGATGATGAGGAAGACATTGCCAGAGTTTTTAATAATTTCTTTCAAGATCTTTTCTCTACAAGTCATCCAGAAAGAATTACTGAAGCTGTGCAAGTGGTCAAAGATAGATTGTCGGATGCTATGCGTAACATATTAGAAGCTGAGTTCACAGAATATGAGGTTTCCTTAGCCGCTAAAAATTTAAAGCCTCATGCTGCTCCGGGACCGGATGGAATGCCTGCTTTATTTTATCAACAATTTTGGAGTATAGTTGGCAAAGATGTGACTTCTTTTGCTTTGAAGATTCTCAATGGTGGGGGTAATCCGAGCAACATTAATTATACATATATTTGTCTTATTCCAAAAAAGAAGAAGCCGAAGGTGCCAGGTGATTTTCGACCTATTTCTCTTTGCAATGTCATTTTTAAAATAATTACGAAAACAATTGCAAATAGGTTGAAATTGATTCTTCCGGATATCATTGGAAAATTCCAAAGTGCTTTTGTGCCGGGACGTTTAATTACTGATAATGCCTTACTAGCTTTTGAATCTTTTCATTATATGAGGAAAAAAAGAAAAGGCAAGAAGGGGTGTGTGGCGTTAAAGCTAGATATGTCAAAAGCATATGATCGTATCGAGTGGAATTTTTTGATTGAGGTGCTTCAGTCTATGGGTTTTTCTCATAAATGGCAAAACTTGATTTTCAATTGTATTTCCACTATTTCTTTCTCTGTTTTGTTGAATGGAAGTCCTTGTCAGAAGTTTTATCCTCAACGAGGCCTCCGTCAAGGGGACCCTTTATCTCCTTATCTATTTATCTTATGTGCTGAGGTTTTTTCCGGGCTTCTAAATAAGGCACAAGCAGAAAAGGGCCTTCATGGAATTCAAATTGCTAGAGGAGCCCCAAAAATTAATCATTTATTTTTTGCAGATGATAGCCTCATTTTCTGTAGGGATTCTTTTCAAGATTCTCAGACCATTCAAGAAATTCTTAATACTTACCAATCTGCTTCTGGTCAACTTATTAATTTGGACAAATCTGAAATCTCTTTTAGCCAAAATGTACCTAATGATCGTAAAATTTTGTTCCAAGGTTGGATGCAAATTAAGGCTGTTGAGTGTCATTCAAAGTATCTGGGGATGCCTGCTTTTGTTGGTAGATCTAAGAAACAGGTTTTCAATTTTGTCCAAGATAGGGTGTGGAAGAAGTTGAAAGGTTGGAAAGAAAAATTTCTATCAACTGCTGGGAGAGAAGTTTTGATCAAATCAGTTGTTCAAGCAATTCCTACGTATGTGATGAGTTGTTTTCTACTTCCGATGGGTTTATGTGAGCATATTGAAAGTATGATTAGTAAGTTCTGGTGGGGTAGCAAACAAGGTGAACGTAAAATTCATTGGATTAAATGGGAGACATTATGTAAGGAAAAAAAGAAAGGTGGAATAGGCTTTCGAACATTTCATGAGTTTAACCTTGCTATGTTGTTTAAACAAGGTTGGCGTATTATGCACAACGACGACTTATTGATCTCACAATGTTTGAAATCAAGGTATTTTCCTAGAAGCAATTTCTTGAAAGCTTCAACTGGTTACTGCCCAAGTTATACATGGAGAAGTATTTTGCAAGCACGAGTGGATATTATTGAGAAAGCTGGTATTTGGAGAGTAGGGGACGGTCATCTAATTAACGTGTGGGAGGATAATTGGCTTCCTTATCAAAATGGACACAAGATTTGGTCCCCTAAACCCCCTAATTGTGATATTATGTTTGTCTCAGATCTCATTCACCATGATATTTGATGTTGGAATGCCCCTTTAATTAATGAAATTTTTCTCCCTTTTGAGGCTCAACAAATTCTTCAATTACCAATTTCATTCTCTCAATTGCAAGATGAATTTGTGTGGGGTGCTTCACGGTCAGCTACTTTTTCTGTGAAGAGTTCATACCATTTCTTAAAGGCAAGGCGTGCAGCAGATGGCTCACATAGCTCAATGCAAGACAATAATCACCAAGTGTGGAAAAAATTATGGAAAATAAAAAGTATTCCAAGACACATCCACTTTTCTTGGAGGATTCTACATGAAAAACTACCAGTGAAAAATGACCTGTTTAAAAAAGGAATAAGTAGTGATCCATTGTGTGTTCTATGTGGGGAACATAATGAGACAATCTCACACCTCTTTATGGAATGTCATTGGTCAAAGCAGATATGGTTTGCTTCCTCTTTAGGGGTGGTATTTGATTCGCATGAGTCCAGATATGTTCCTTTTCATAAGTGGTTAGAGAAAAATATTATTGATGTCGATGAGGATATTATAGTACATGTTCTGGCCTTGTGTTATGAGATATGGTGTGCTAGAAATAAGAAATGTTTTGAGGGGACTGATGTTGATGTGACAGCCACTATTCAAAAGGCACAAAGATCTATTGTGAATTTTAAGAGTGCTAGTACGGTGTTGGTGGAGACACTAAGTGGAGGTCCAATTTTACCAATATATGATGTTCATTGGACAACTCTGTTTAGTGATTTCTATAAGCTTAATGTTGACGCAGCAGGTCCGATAGAGGGTGATAAGTGGGGTATTGGTGTTGTGGTGAGAGATAATGAAGGTGTTGTCGTTGGTGCTAGTTGCTGGCAAGTCTGTTCATTACCAGATTCGGAGGCTGCAGAAGCTTTAGCTATGCGGAAGGGTTTGGAATTTGCAAAAGACATGTCTTTTCTGAATCTTATAGAAGAATCAGATGCTTCTAATGTGGTTTTAGCGTTGAATGCTCATCAACAATCTCCCAACTATGTTGGCTCTATTATTCGAGATTGTATTAGTTTTAAGGGTAGTTTTCGTAGTTTAAATTTTTTGCATGTTAGGCGTGAAGCCAACCAAGCTGCTCATTATTTAGCTAAATATGCTCTTAGAAATTTAGATTGCATATGGGTCGAAGAAACTCCGCCTTGTATTTCTGCAGTCTTGGCATTTGATTTATTGCCAGAATTTCGTTAATGAAATTGTCTTTAACGTCAAAAAAAAAAGATGAAGAAGGAAAAGTGACTCGCAAAGATATCAGAACTGGTTGTTCAGCGCATTTGGTCATTGGTCGAGAATCCAATGGAAAATATGTGGTTACATCTTTTGTCAAAGACCATAACCATGAACTTGCAACCCCCAGGAGTAGGCATAAGTTACCATCACAAAGAAAAGTTTATGTCACTCAAGCTACTGAAGTTGAAATGGCGAACCGGTCCGGCATACGTCAGAAACTAATTTTTGAGTTCATTAGTCGACAAGTTGGAGGGAGAGAAAATGTTGGTTGCACTGCTAAAGATATTAGCAATCATTTAAATTCTAAAAGGATGAAGGAAATGGAGGAAGGTGAGGCATATACTCTTCTTCATTATTTTAAATCAAAGCAATCAGAGAATTCATCATTCTTTTATGAAATTCAACTAGACATTGATAATCTAATAACAAACATCTTTTGGGCTGATGCTAAAATGGTTGTGGATTATGCTTATTTTGGAGATGTAGTGTGTTTTGATACCACTTATCGCACTAATAAGAATTTACGACCATTTGCTCCGTTTGTTGGATTTAATCATCATCGAGAAAGTGTGTTATTCGGTGCAGCATTGTTATGTGATGAGACAGCTGAATCATTTAAGTGGTTGTTCAAGACTTTTCTTAAAGCTATGTGTGGTAAAAAGCCGATGACAATTTTAACAGATCAGGATCCTGCAATGGCAAAAGCAATTTCAGAGGTTTTTCCCGAGTCATATCATCGTTTATGCTTGTGGCATTTATTTCAAAATGCACTAAAGAATGTTAATCATGCTTTTCAGCGATCTAATTATTTTGCTAGCGAGTTGCAAAGTTGCATTTATGGTTTCGAGCATGCGGATGATTTTCTGAATGCTTGGGAGTCATTACTTGACAAGCATAATCTTCGCCAAAATAAATGGATGCAAGATTTCTTTAACAAAAAGGAAAAATGGGCTTCGGTATATGGGCGACACACTTTTTCAGCCGGTGCTACAACAACACAATTAAGTGAGAGCTTTAATGGCCGTTTAAGGCTTTACATGAAGCCTACCTTTAATGTGTTAGAATTTTTCAGAAATTTTGAGAGATTGCTAGATGATATGCTATATAAGGAGGTAGAGTCTAAATATGAAATGAGCCAGAAGATGCCATCATTGAATATGAACATCATGTTATTGAGAAATGCAAGAGATGTCTATAAACCGACAATTTTTTCTCTTGTTCGTGGGGAATATGAAAAATCATGTGATCTTGTTTTGGTAATATGCACGCAGAACTTAATGTTATATGAATACGATGTATGCTTGTTTGGGGCTATGAGGAAGCATAAAGTTACCTTCAACTCTGAAGATCAAAGTGTTGAGTGTAGCTGTCAACTTTTTCAATTTGTTGGTATCCTTTGCTGCCATGCTATTCGAGTACTTAATCATTGGAATATAATTGTTATTCCTCCAAAATACATCCTTAAGAGATGGACCAAGAATGCTCGAAGTGGATGTGTTCTTGACAATAAAGGACAAATCATCAAAGAAGATCCTAAGTTAGTTGTCTTAGATCGCTCTAAGGATTTGTGTCGCACTACCGTAGCAATATCATGTAAGGCTGCTAATTCTGAAGATGTTTCGTCATATTTAGCTAAAAAATTGGTGGAAATTGGACTTGAAGTTGAAAACATCTCGAGTAAAAGGTCTTCCATACAGAGTGCAGATCATGGTGATATAACTTGTCAAACAAATGATGTTAACTTTGATGACAGAACAATTGCTTTGAATGAAAACTTCAATGCATCTGAAGTTATGGGTATAAAAAAGAAGGATGGTGTATCCAGAGGAAAAGGTAGACCTAAGAGTTGTCTAGAGAGAAAATATAAAAGAAAAAAAAGACACTCAAAAGTATAGTTCTGTGAGACATAATTTTTCTCCCACCATGCCAATTCCAATTATACAGGTAGAGCAAGGTACTACTTATACAGCTACAACAAGACAGGATGTATTGTTTTCACATGGATGTCAAGATACACTAGCAATTGGTATTAA
Protein sequence

>MS.gene32378.t1

MVINIGDLIPTHPSCEVLGSNVNESRNCNTAPATSDSNVKFKDNIENDVTLDDIVPNVGMEFDTEDHAYRFYNMYAGFVGFSVRKDWRNKNFEMNKFRNVQGLSGVVAVSCSGEGNRRAGGLAMFWKEGVEVEVLSISSNHIDVLIAIENGDEKWRLTGVYGFPESHMKHKTCELIDQLALVESTDKWLLMGDFNLILSQDEKEGGNFGNSHMISMFRDTLQNYNLSDLGFEGDKFTWHNRQDGVSNIKARLDRMIATPAWSSFYPSASVVHLTRYASDHLPLWLRLASRKPKKKQNVLKLQRFEECWLRDCGILDAVKGSWEVAGDSMSDKINRCVNDLSNWSKDRFGDVPNQIKNVQKKLDDLNKQIQHEGVMSEIRRVETRLNDLVENEEGKVTRKDIRTGCSAHLVIGRESNGKYVVTSFVKDHNHELATPRSRHKLPSQRKVYVTQATEVEMANRSGIRQKLIFEFISRQVGGRENVGCTAKDISNHLNSKRMKEMEEAGATTTQLSESFNGRLRLYMKPTFNVLEFFRNFERLLDDMLYKEVESKYEMSQKMPSLNMNIMLLRNARDVYKPTIFSLVRGEYEKSCDLVLVICTQNLMLYEYDVCLFGAMRKHKVTFNSEDQSVECSCQLFQFVGILCCHAIRVLNHWNIIVIPPKYILKRWTKNARSGCVLDNKGQIIKEDPKLVVLDRSKDLCRTTVAISCKAANSEDVSSYLAKKLVEIGLEVENISSKRSSIQSADHGDITCQTNDVNFDDRTIALNENFNASEVMGIKKKDGVSRGKGRARYYLYSYNKTGCIVFTWMSRYTSNWY