AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene32455

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32455.t1 MTR_1g006650 94.477 344 15 2 1 340 1 344 0.0 646
MS.gene32455.t1 MTR_3g109760 55.152 330 107 8 19 340 22 318 2.63e-104 309
MS.gene32455.t1 MTR_8g013950 46.291 337 133 12 23 340 35 342 2.74e-77 241
MS.gene32455.t1 MTR_8g076340 45.854 205 93 5 96 300 102 288 1.86e-45 157
MS.gene32455.t1 MTR_4g071590 34.615 312 141 10 5 299 15 280 7.14e-44 153
MS.gene32455.t1 MTR_4g471590 34.615 312 141 10 5 299 15 280 7.14e-44 153
MS.gene32455.t1 MTR_3g117380 38.140 215 115 3 87 300 102 299 1.04e-39 142
MS.gene32455.t1 MTR_6g032830 38.696 230 106 7 101 296 30 258 2.13e-39 140
MS.gene32455.t1 MTR_5g021340 43.723 231 96 7 94 300 150 370 2.55e-39 143
MS.gene32455.t1 MTR_1g094930 35.144 313 143 11 27 294 9 306 2.12e-37 137
MS.gene32455.t1 MTR_1g046680 78.873 71 15 0 229 299 171 241 1.36e-34 127
MS.gene32455.t1 MTR_5g007600 74.648 71 18 0 229 299 258 328 5.82e-33 125
MS.gene32455.t1 MTR_5g007600 72.222 72 20 0 229 300 156 227 1.44e-29 115
MS.gene32455.t1 MTR_7g085410 39.545 220 93 8 105 294 43 252 5.96e-33 123
MS.gene32455.t1 MTR_1g094930 78.462 65 14 0 230 294 147 211 1.16e-32 122
MS.gene32455.t1 MTR_1g116010 55.963 109 43 2 209 316 142 246 7.15e-32 120
MS.gene32455.t1 MTR_2g094600 76.923 65 15 0 230 294 216 280 1.80e-31 120
MS.gene32455.t1 MTR_4g058910 73.134 67 18 0 230 296 314 380 3.02e-30 119
MS.gene32455.t1 MTR_7g446110 62.903 62 23 0 230 291 187 248 3.76e-21 91.3
MS.gene32455.t1 MTR_7g445950 58.065 62 26 0 230 291 120 181 5.53e-19 84.0
MS.gene32455.t1 MTR_7g446070 47.059 85 44 1 207 291 110 193 1.02e-18 83.2
MS.gene32455.t1 MTR_7g445980 62.712 59 21 1 234 291 37 95 1.58e-18 80.1
MS.gene32455.t1 MTR_7g112330 70.588 34 10 0 232 265 24 57 2.83e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32455.t1 AT5G25830 56.226 265 99 7 93 340 67 331 4.29e-90 273
MS.gene32455.t1 AT4G32890 43.284 335 133 9 21 340 16 308 9.45e-79 243
MS.gene32455.t1 AT3G60530 47.826 207 64 6 93 298 63 226 3.41e-49 165
MS.gene32455.t1 AT2G45050 42.857 238 80 7 89 310 60 257 9.46e-49 164
MS.gene32455.t1 AT5G66320 38.889 270 115 8 69 312 86 331 9.04e-41 146
MS.gene32455.t1 AT5G66320 38.889 270 115 8 69 312 86 331 9.04e-41 146
MS.gene32455.t1 AT4G36240 36.140 285 110 9 22 297 10 231 1.48e-36 132
MS.gene32455.t1 AT4G34680 38.249 217 83 5 90 299 77 249 4.67e-36 132
MS.gene32455.t1 AT4G34680 38.249 217 83 5 90 299 77 249 4.67e-36 132
MS.gene32455.t1 AT3G51080 33.981 309 158 10 6 298 11 289 1.98e-34 128
MS.gene32455.t1 AT3G24050 74.286 70 18 0 229 298 193 262 2.80e-33 124
MS.gene32455.t1 AT1G08010 65.476 84 23 1 225 302 215 298 4.32e-33 124
MS.gene32455.t1 AT1G08010 65.476 84 23 1 225 302 215 298 4.32e-33 124
MS.gene32455.t1 AT1G08010 65.476 84 23 1 225 302 215 298 4.32e-33 124
MS.gene32455.t1 AT1G08000 70.833 72 21 0 225 296 213 284 4.55e-31 119
MS.gene32455.t1 AT1G08000 70.833 72 21 0 225 296 213 284 4.55e-31 119
MS.gene32455.t1 AT3G54810 62.963 81 30 0 230 310 229 309 6.18e-31 119
MS.gene32455.t1 AT3G54810 62.963 81 30 0 230 310 229 309 6.18e-31 119
MS.gene32455.t1 AT3G45170 43.846 130 62 2 178 298 56 183 1.19e-26 105
MS.gene32455.t1 AT2G28340 69.841 63 19 0 232 294 193 255 1.38e-26 107

Find 118 sgRNAs with CRISPR-Local

Find 146 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCGTCACTGTCCTTTCTTCT+TGG 0.090798 1.4:+12230553 None:intergenic
AAAACGATTCCTCATCAATA+TGG 0.237248 1.4:+12230866 None:intergenic
TCAAAAGATGCAACTGATAT+CGG 0.281999 1.4:-12230830 MS.gene32455:CDS
CCAATATCAGGAACGACATT+AGG 0.287162 1.4:+12231448 None:intergenic
GCAGTCTTTGCCTGCTTCCT+TGG 0.291400 1.4:+12230577 None:intergenic
TACTCTGATGTTGTAAAATC+TGG 0.292416 1.4:+12230275 None:intergenic
TATATTCAACAAGGAAGTTT+TGG 0.298704 1.4:-12230743 MS.gene32455:CDS
CTAGCTGCAGGCCTGTATTC+AGG 0.333508 1.4:+12230397 None:intergenic
CTCCGGAGACCTGTGTGTTC+CGG 0.345236 1.4:-12231398 MS.gene32455:intron
CCTAATGTCGTTCCTGATAT+TGG 0.361673 1.4:-12231448 MS.gene32455:CDS
AAAACTTCCTTGTTGAATAT+AGG 0.362486 1.4:+12230745 None:intergenic
CGACGTCATTGTTGAGGTTA+AGG 0.370723 1.4:+12231593 None:intergenic
TGTTGAATATAGGACTGTTT+CGG 0.382615 1.4:+12230755 None:intergenic
AAGACACCACAATGGCGAAC+CGG 0.386976 1.4:-12230484 MS.gene32455:CDS
ACGATGCGAGTTGGAGTGTT+TGG 0.387998 1.4:+12230357 None:intergenic
AAATTATGACAGCCAATATC+AGG 0.396891 1.4:+12231436 None:intergenic
TTACTTGATTCACCAACATA+TGG 0.405233 1.4:-12230224 MS.gene32455:CDS
AGGACTGTTTCGGTTGGGTT+GGG 0.406165 1.4:+12230765 None:intergenic
GTTGGGTTGGGAAAGCTCAC+GGG 0.412320 1.4:+12230777 None:intergenic
CAATGGCGAACCGGGCCTAA+TGG 0.414512 1.4:-12230475 MS.gene32455:CDS
CACACAGGTCTCCGGAGAAA+TGG 0.416213 1.4:+12231404 None:intergenic
GGAGGATGACTTAGCGGCCC+TGG 0.433321 1.4:-12230899 MS.gene32455:CDS
CACGGTAACGGTGGAGGAAG+AGG 0.434827 1.4:+12231510 None:intergenic
CAATATCAGGAACGACATTA+GGG 0.440367 1.4:+12231449 None:intergenic
CTCTTGCTACGGGCCTTAGC+TGG 0.442400 1.4:+12230718 None:intergenic
TGTCGGAGGAAAAGGTGGTT+TGG 0.443866 1.4:+12231650 None:intergenic
GGTTGGGTTGGGAAAGCTCA+CGG 0.448750 1.4:+12230776 None:intergenic
ATGACTTAGCGGCCCTGGAC+TGG 0.449566 1.4:-12230894 MS.gene32455:CDS
TACATCATCTGCCTAAAATC+AGG 0.450652 1.4:+12230199 None:intergenic
AATATAGGACTGTTTCGGTT+GGG 0.459476 1.4:+12230760 None:intergenic
TAGGACTGTTTCGGTTGGGT+TGG 0.462649 1.4:+12230764 None:intergenic
AGTGTTGTTGTCGGAGGAAA+AGG 0.462952 1.4:+12231642 None:intergenic
AAGGACAGTGACGGAGGGTC+AGG 0.466229 1.4:-12230544 MS.gene32455:CDS
GAGGCAGAAGGAAATGTTGC+GGG 0.466463 1.4:-12230317 MS.gene32455:CDS
TACTTGATTCACCAACATAT+GGG 0.466490 1.4:-12230223 MS.gene32455:CDS
GTCGATTGGTGCCTGAATAC+AGG 0.470023 1.4:-12230408 MS.gene32455:CDS
AGACACCACAATGGCGAACC+GGG 0.471491 1.4:-12230483 MS.gene32455:CDS
GCCCACGAGTCCTCTTGCTA+CGG 0.473059 1.4:+12230707 None:intergenic
GAATATAGGACTGTTTCGGT+TGG 0.481982 1.4:+12230759 None:intergenic
GCTCACGGGGTTCTTCATCC+TGG 0.486213 1.4:+12230791 None:intergenic
GTTAGCTCGTCATCATTGTC+CGG 0.486841 1.4:-12231478 MS.gene32455:CDS
CAAAAGATGCAACTGATATC+GGG 0.494290 1.4:-12230829 MS.gene32455:CDS
AAACGATTCCTCATCAATAT+GGG 0.498163 1.4:+12230867 None:intergenic
GACGTGAGCTCCAGTCGCAA+GGG 0.498267 1.4:+12230683 None:intergenic
AATCAAGTAATCATCATTGT+TGG 0.499207 1.4:+12230237 None:intergenic
TGTTTGGTCAGCACAAATGT+AGG 0.504596 1.4:+12230373 None:intergenic
ATCGTAAAGTGTTGGAGCTA+CGG 0.505579 1.4:-12230339 MS.gene32455:CDS
AAGGTTGGTGTCGGAAATGA+TGG 0.507635 1.4:+12231558 None:intergenic
GGTGGTTTGGAAAATGTTGT+TGG 0.508256 1.4:+12231663 None:intergenic
TACCGACAATTTCATTGTTG+AGG 0.524862 1.4:-12231620 MS.gene32455:CDS
TACCGGAACACACAGGTCTC+CGG 0.525459 1.4:+12231396 None:intergenic
ATTGACCACGGTAACGGTGG+AGG 0.527850 1.4:+12231504 None:intergenic
GCCCGTAGCAAGAGGACTCG+TGG 0.533598 1.4:-12230709 MS.gene32455:CDS
AAGATACAAGTCGGGTCGAT+TGG 0.534399 1.4:-12230422 MS.gene32455:CDS
CCCACGAGTCCTCTTGCTAC+GGG 0.537511 1.4:+12230708 None:intergenic
GGGCATGAAGGTGCGAAACC+AGG 0.537637 1.4:-12230809 MS.gene32455:CDS
GACGGAGGGTCAGGTGGAGA+TGG 0.539597 1.4:-12230535 MS.gene32455:CDS
GTTGTTGTCGGAGGAAAAGG+TGG 0.541025 1.4:+12231645 None:intergenic
TGGAACAGTGACGGTAGGTA+AGG 0.543018 1.4:+12230597 None:intergenic
AACAGTGACGGTAGGTAAGG+TGG 0.543787 1.4:+12230600 None:intergenic
GAGGGTCAGGTGGAGATGGT+CGG 0.544742 1.4:-12230531 MS.gene32455:CDS
CGTCACTGTTCCAAGGAAGC+AGG 0.545318 1.4:-12230587 MS.gene32455:CDS
CAACACTTTACGATGCGAGT+TGG 0.550252 1.4:+12230348 None:intergenic
CAATATGGGAAATCCAGTCC+AGG 0.555569 1.4:+12230881 None:intergenic
AAAGACTGCTCCAAGAAGAA+AGG 0.555772 1.4:-12230563 MS.gene32455:CDS
AACGCTATTGACCACGGTAA+CGG 0.557909 1.4:+12231498 None:intergenic
GACAGTGACGGAGGGTCAGG+TGG 0.558691 1.4:-12230541 MS.gene32455:CDS
AGTTGATTGTGGCTGAGTCA+AGG 0.559211 1.4:+12231539 None:intergenic
ACAAATGTAGGGCTAGCTGC+AGG 0.560737 1.4:+12230385 None:intergenic
AGACGTGAGCTCCAGTCGCA+AGG 0.560751 1.4:+12230682 None:intergenic
ACAGTGACGGTAGGTAAGGT+GGG 0.561238 1.4:+12230601 None:intergenic
GAAATAGGAGGATGACTTAG+CGG 0.565643 1.4:-12230905 MS.gene32455:intron
GTCCTCAACAATGAAATTGT+CGG 0.566137 1.4:+12231618 None:intergenic
GCAACTGATATCGGGCATGA+AGG 0.569194 1.4:-12230821 MS.gene32455:CDS
TAAAATCAGGTCCCATATGT+TGG 0.573858 1.4:+12230212 None:intergenic
GTTTGGTCAGCACAAATGTA+GGG 0.574012 1.4:+12230374 None:intergenic
CCCGTAGCAAGAGGACTCGT+GGG 0.574146 1.4:-12230708 MS.gene32455:CDS
GATTGTGGCTGAGTCAAGGT+TGG 0.578853 1.4:+12231543 None:intergenic
TTGGGTTGGGAAAGCTCACG+GGG 0.578980 1.4:+12230778 None:intergenic
CAGCTAAGGCCCGTAGCAAG+AGG 0.579929 1.4:-12230717 MS.gene32455:CDS
TTAGGCCCGGTTCGCCATTG+TGG 0.587065 1.4:+12230478 None:intergenic
TTAGCTCGTCATCATTGTCC+GGG 0.587117 1.4:-12231477 MS.gene32455:CDS
TTGTGGTGTAAGATACAAGT+CGG 0.589234 1.4:-12230431 MS.gene32455:CDS
CTGCTTCCTTGGAACAGTGA+CGG 0.590093 1.4:+12230588 None:intergenic
ATTCCCTACGTAGGAATGGA+AGG 0.590186 1.4:-12231691 None:intergenic
ATTGTCGGTAGTGTTGTTGT+CGG 0.590207 1.4:+12231633 None:intergenic
GGTCAGGTGGAGATGGTCGG+AGG 0.590443 1.4:-12230528 MS.gene32455:CDS
GTCGGTAGTGTTGTTGTCGG+AGG 0.590547 1.4:+12231636 None:intergenic
TACCTACCGTCACTGTTCCA+AGG 0.593877 1.4:-12230594 MS.gene32455:CDS
CGTCGTCGACGTCATTGTTG+AGG 0.596242 1.4:+12231587 None:intergenic
CTGGATTTCCCATATTGATG+AGG 0.600285 1.4:-12230875 MS.gene32455:CDS
AACGACATTAGGGTCGCACC+CGG 0.603894 1.4:+12231459 None:intergenic
CAACTCGCATCGTAAAGTGT+TGG 0.604228 1.4:-12230347 MS.gene32455:CDS
CTCTTCCTCCACCGTTACCG+TGG 0.606184 1.4:-12231509 MS.gene32455:CDS
GGCTGAGTCAAGGTTGGTGT+CGG 0.607066 1.4:+12231549 None:intergenic
TGTGGTGTAAGATACAAGTC+GGG 0.608929 1.4:-12230430 MS.gene32455:CDS
GTTGGAGCTACGGAGGCAGA+AGG 0.611025 1.4:-12230329 MS.gene32455:CDS
CGTCATTGTTGAGGTTAAGG+AGG 0.612987 1.4:+12231596 None:intergenic
ACGTGAGCTCCAGTCGCAAG+GGG 0.619626 1.4:+12230684 None:intergenic
GCTATTGACCACGGTAACGG+TGG 0.631846 1.4:+12231501 None:intergenic
GAAGAAAGGACAGTGACGGA+GGG 0.637953 1.4:-12230549 MS.gene32455:CDS
AAAACATTATGCAACGCTTG+TGG 0.638941 1.4:-12230448 MS.gene32455:CDS
CCAAGAAGAAAGGACAGTGA+CGG 0.640630 1.4:-12230553 MS.gene32455:CDS
AGAAGAAAGGACAGTGACGG+AGG 0.643844 1.4:-12230550 MS.gene32455:CDS
CAGTGACGGTAGGTAAGGTG+GGG 0.651207 1.4:+12230602 None:intergenic
AATATGGGAAATCCAGTCCA+GGG 0.656494 1.4:+12230882 None:intergenic
AGAGGCGGTTGAGTTGATTG+TGG 0.662222 1.4:+12231528 None:intergenic
GTAAAGTGTTGGAGCTACGG+AGG 0.667005 1.4:-12230336 MS.gene32455:CDS
GGAGGCAGAAGGAAATGTTG+CGG 0.675658 1.4:-12230318 MS.gene32455:CDS
AGTTCTGACTCAGAAGAAGA+CGG 0.679246 1.4:+12230637 None:intergenic
TTCCTTGGAACAGTGACGGT+AGG 0.684302 1.4:+12230592 None:intergenic
GGTAACGGTGGAGGAAGAGG+CGG 0.684503 1.4:+12231513 None:intergenic
AGTGACGGTAGGTAAGGTGG+GGG 0.685489 1.4:+12230603 None:intergenic
TATAATATACCGGAACACAC+AGG 0.691414 1.4:+12231389 None:intergenic
AAACAGTCCTATATTCAACA+AGG 0.707758 1.4:-12230752 MS.gene32455:CDS
CGAGCTAACGCTATTGACCA+CGG 0.719172 1.4:+12231492 None:intergenic
CGTGAGCTCCAGTCGCAAGG+GGG 0.723454 1.4:+12230685 None:intergenic
TGACAGACAAGACACCACAA+TGG 0.739729 1.4:-12230492 MS.gene32455:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CATATACACATATAATATAC+CGG + chr1.4:12230498-12230517 None:intergenic 20.0%
!!! TTTGTGTTGAAATTTGAAAT+AGG - chr1.4:12230954-12230973 MS.gene32455:intron 20.0%
!!! TTTTGTGATACTCAATAATT+TGG + chr1.4:12230800-12230819 None:intergenic 20.0%
! AAAACTTCCTTGTTGAATAT+AGG + chr1.4:12231132-12231151 None:intergenic 25.0%
! AATCAAGTAATCATCATTGT+TGG + chr1.4:12231640-12231659 None:intergenic 25.0%
! CTGAAATTATTGGCAAAAAA+GGG + chr1.4:12230682-12230701 None:intergenic 25.0%
!! TATATTCAACAAGGAAGTTT+TGG - chr1.4:12231131-12231150 MS.gene32455:intron 25.0%
!!! GTTTTTTCTCCTGAAATTAT+TGG + chr1.4:12230692-12230711 None:intergenic 25.0%
AAAACGATTCCTCATCAATA+TGG + chr1.4:12231011-12231030 None:intergenic 30.0%
AAACAGTCCTATATTCAACA+AGG - chr1.4:12231122-12231141 MS.gene32455:intron 30.0%
AAACGATTCCTCATCAATAT+GGG + chr1.4:12231010-12231029 None:intergenic 30.0%
AAATTATGACAGCCAATATC+AGG + chr1.4:12230441-12230460 None:intergenic 30.0%
CCTGAAATTATTGGCAAAAA+AGG + chr1.4:12230683-12230702 None:intergenic 30.0%
GCATGAGTTAGAAATTTACA+TGG + chr1.4:12230617-12230636 None:intergenic 30.0%
TACTCTGATGTTGTAAAATC+TGG + chr1.4:12231602-12231621 None:intergenic 30.0%
TACTTGATTCACCAACATAT+GGG - chr1.4:12231651-12231670 MS.gene32455:CDS 30.0%
TAGGCTAAATACAACAGAAT+GGG - chr1.4:12230719-12230738 MS.gene32455:CDS 30.0%
TCAAAAGATGCAACTGATAT+CGG - chr1.4:12231044-12231063 MS.gene32455:intron 30.0%
TGTTGAATATAGGACTGTTT+CGG + chr1.4:12231122-12231141 None:intergenic 30.0%
TTACTTGATTCACCAACATA+TGG - chr1.4:12231650-12231669 MS.gene32455:CDS 30.0%
! GTGTTGAAATTTGAAATAGG+AGG - chr1.4:12230957-12230976 MS.gene32455:intron 30.0%
! TGTTTCAAGAGCATATATAG+AGG - chr1.4:12230920-12230939 MS.gene32455:intron 30.0%
!!! CCTTTTTTGCCAATAATTTC+AGG - chr1.4:12230680-12230699 MS.gene32455:CDS 30.0%
!!! GTTGTAAAATCTGGTTTTGT+TGG + chr1.4:12231593-12231612 None:intergenic 30.0%
AAAACATTATGCAACGCTTG+TGG - chr1.4:12231426-12231445 MS.gene32455:CDS 35.0%
AATATAGGACTGTTTCGGTT+GGG + chr1.4:12231117-12231136 None:intergenic 35.0%
AGGAGAAAAAACAACTAGCT+AGG - chr1.4:12230700-12230719 MS.gene32455:CDS 35.0%
AGGCTAAATACAACAGAATG+GGG - chr1.4:12230720-12230739 MS.gene32455:CDS 35.0%
CAAAAGATGCAACTGATATC+GGG - chr1.4:12231045-12231064 MS.gene32455:intron 35.0%
CAATATCAGGAACGACATTA+GGG + chr1.4:12230428-12230447 None:intergenic 35.0%
CTAGGCTAAATACAACAGAA+TGG - chr1.4:12230718-12230737 MS.gene32455:CDS 35.0%
GTCCTCAACAATGAAATTGT+CGG + chr1.4:12230259-12230278 None:intergenic 35.0%
TAAAATCAGGTCCCATATGT+TGG + chr1.4:12231665-12231684 None:intergenic 35.0%
TACATCATCTGCCTAAAATC+AGG + chr1.4:12231678-12231697 None:intergenic 35.0%
TACCGACAATTTCATTGTTG+AGG - chr1.4:12230254-12230273 MS.gene32455:CDS 35.0%
TATAATATACCGGAACACAC+AGG + chr1.4:12230488-12230507 None:intergenic 35.0%
TTGTGGTGTAAGATACAAGT+CGG - chr1.4:12231443-12231462 MS.gene32455:CDS 35.0%
! AACATATGGGACCTGATTTT+AGG - chr1.4:12231664-12231683 MS.gene32455:CDS 35.0%
!! AAGCGTTGCATAATGTTTTC+GGG + chr1.4:12231425-12231444 None:intergenic 35.0%
!! CAAGCGTTGCATAATGTTTT+CGG + chr1.4:12231426-12231445 None:intergenic 35.0%
!!! AAATCTGGTTTTGTTGGTGT+TGG + chr1.4:12231587-12231606 None:intergenic 35.0%
AAAGACTGCTCCAAGAAGAA+AGG - chr1.4:12231311-12231330 MS.gene32455:intron 40.0%
AATATGGGAAATCCAGTCCA+GGG + chr1.4:12230995-12231014 None:intergenic 40.0%
CCAATATCAGGAACGACATT+AGG + chr1.4:12230429-12230448 None:intergenic 40.0%
CCTAATGTCGTTCCTGATAT+TGG - chr1.4:12230426-12230445 MS.gene32455:CDS 40.0%
CTGGATTTCCCATATTGATG+AGG - chr1.4:12230999-12231018 MS.gene32455:intron 40.0%
GAAATAGGAGGATGACTTAG+CGG - chr1.4:12230969-12230988 MS.gene32455:intron 40.0%
GAATATAGGACTGTTTCGGT+TGG + chr1.4:12231118-12231137 None:intergenic 40.0%
GTTTGGTCAGCACAAATGTA+GGG + chr1.4:12231503-12231522 None:intergenic 40.0%
TGTGGTGTAAGATACAAGTC+GGG - chr1.4:12231444-12231463 MS.gene32455:CDS 40.0%
TGTTTGGTCAGCACAAATGT+AGG + chr1.4:12231504-12231523 None:intergenic 40.0%
! AGTTCTGACTCAGAAGAAGA+CGG + chr1.4:12231240-12231259 None:intergenic 40.0%
! ATTGTCGGTAGTGTTGTTGT+CGG + chr1.4:12230244-12230263 None:intergenic 40.0%
! GGTGGTTTGGAAAATGTTGT+TGG + chr1.4:12230214-12230233 None:intergenic 40.0%
! TTTTCTGACAGCCATTTCTC+CGG - chr1.4:12230459-12230478 MS.gene32455:CDS 40.0%
!! ATCGTAAAGTGTTGGAGCTA+CGG - chr1.4:12231535-12231554 MS.gene32455:CDS 40.0%
!! CATAATGTTTTCGGGCCATT+AGG + chr1.4:12231417-12231436 None:intergenic 40.0%
AACGCTATTGACCACGGTAA+CGG + chr1.4:12230379-12230398 None:intergenic 45.0%
AAGATACAAGTCGGGTCGAT+TGG - chr1.4:12231452-12231471 MS.gene32455:CDS 45.0%
ACAGAATGGGGCACATTTCA+CGG - chr1.4:12230732-12230751 MS.gene32455:CDS 45.0%
AGTTGATTGTGGCTGAGTCA+AGG + chr1.4:12230338-12230357 None:intergenic 45.0%
CAACACTTTACGATGCGAGT+TGG + chr1.4:12231529-12231548 None:intergenic 45.0%
CAACTCGCATCGTAAAGTGT+TGG - chr1.4:12231527-12231546 MS.gene32455:CDS 45.0%
CAATATGGGAAATCCAGTCC+AGG + chr1.4:12230996-12231015 None:intergenic 45.0%
CCAAGAAGAAAGGACAGTGA+CGG - chr1.4:12231321-12231340 MS.gene32455:intron 45.0%
CGACGTCATTGTTGAGGTTA+AGG + chr1.4:12230284-12230303 None:intergenic 45.0%
CGTCATTGTTGAGGTTAAGG+AGG + chr1.4:12230281-12230300 None:intergenic 45.0%
GTTAGCTCGTCATCATTGTC+CGG - chr1.4:12230396-12230415 MS.gene32455:CDS 45.0%
TGACAGACAAGACACCACAA+TGG - chr1.4:12231382-12231401 MS.gene32455:intron 45.0%
TTAGCTCGTCATCATTGTCC+GGG - chr1.4:12230397-12230416 MS.gene32455:CDS 45.0%
! AGTGTTGTTGTCGGAGGAAA+AGG + chr1.4:12230235-12230254 None:intergenic 45.0%
!! AAGGTTGGTGTCGGAAATGA+TGG + chr1.4:12230319-12230338 None:intergenic 45.0%
!! TGAGGAATCGTTTTCAAGCG+AGG - chr1.4:12231017-12231036 MS.gene32455:intron 45.0%
!!! GGAAGTTTTGGTTCCAGCTA+AGG - chr1.4:12231143-12231162 MS.gene32455:intron 45.0%
AACAGTGACGGTAGGTAAGG+TGG + chr1.4:12231277-12231296 None:intergenic 50.0%
AAGACACCACAATGGCGAAC+CGG - chr1.4:12231390-12231409 MS.gene32455:intron 50.0%
ACAGTGACGGTAGGTAAGGT+GGG + chr1.4:12231276-12231295 None:intergenic 50.0%
AGAAGAAAGGACAGTGACGG+AGG - chr1.4:12231324-12231343 MS.gene32455:intron 50.0%
AGGACTGTTTCGGTTGGGTT+GGG + chr1.4:12231112-12231131 None:intergenic 50.0%
CCGTCACTGTCCTTTCTTCT+TGG + chr1.4:12231324-12231343 None:intergenic 50.0%
CGAGCTAACGCTATTGACCA+CGG + chr1.4:12230385-12230404 None:intergenic 50.0%
CTGCTTCCTTGGAACAGTGA+CGG + chr1.4:12231289-12231308 None:intergenic 50.0%
GAGGCAGAAGGAAATGTTGC+GGG - chr1.4:12231557-12231576 MS.gene32455:CDS 50.0%
GATTGTGGCTGAGTCAAGGT+TGG + chr1.4:12230334-12230353 None:intergenic 50.0%
GCAACTGATATCGGGCATGA+AGG - chr1.4:12231053-12231072 MS.gene32455:intron 50.0%
GGAGGCAGAAGGAAATGTTG+CGG - chr1.4:12231556-12231575 MS.gene32455:CDS 50.0%
GTTGTTGTCGGAGGAAAAGG+TGG + chr1.4:12230232-12230251 None:intergenic 50.0%
TACCTACCGTCACTGTTCCA+AGG - chr1.4:12231280-12231299 MS.gene32455:intron 50.0%
TAGGACTGTTTCGGTTGGGT+TGG + chr1.4:12231113-12231132 None:intergenic 50.0%
TGGAACAGTGACGGTAGGTA+AGG + chr1.4:12231280-12231299 None:intergenic 50.0%
TGTCGGAGGAAAAGGTGGTT+TGG + chr1.4:12230227-12230246 None:intergenic 50.0%
TTCCTTGGAACAGTGACGGT+AGG + chr1.4:12231285-12231304 None:intergenic 50.0%
! ACGATGCGAGTTGGAGTGTT+TGG + chr1.4:12231520-12231539 None:intergenic 50.0%
! GAAGAAAGGACAGTGACGGA+GGG - chr1.4:12231325-12231344 MS.gene32455:intron 50.0%
! GTCGATTGGTGCCTGAATAC+AGG - chr1.4:12231466-12231485 MS.gene32455:CDS 50.0%
!! ACAAATGTAGGGCTAGCTGC+AGG + chr1.4:12231492-12231511 None:intergenic 50.0%
!! AGAGGCGGTTGAGTTGATTG+TGG + chr1.4:12230349-12230368 None:intergenic 50.0%
!! GTAAAGTGTTGGAGCTACGG+AGG - chr1.4:12231538-12231557 MS.gene32455:CDS 50.0%
AACGACATTAGGGTCGCACC+CGG + chr1.4:12230418-12230437 None:intergenic 55.0%
AGACACCACAATGGCGAACC+GGG - chr1.4:12231391-12231410 MS.gene32455:intron 55.0%
AGTGACGGTAGGTAAGGTGG+GGG + chr1.4:12231274-12231293 None:intergenic 55.0%
CACACAGGTCTCCGGAGAAA+TGG + chr1.4:12230473-12230492 None:intergenic 55.0%
CAGTGACGGTAGGTAAGGTG+GGG + chr1.4:12231275-12231294 None:intergenic 55.0%
CGTCACTGTTCCAAGGAAGC+AGG - chr1.4:12231287-12231306 MS.gene32455:intron 55.0%
CGTCGTCGACGTCATTGTTG+AGG + chr1.4:12230290-12230309 None:intergenic 55.0%
CTAGCTGCAGGCCTGTATTC+AGG + chr1.4:12231480-12231499 None:intergenic 55.0%
GCAGTCTTTGCCTGCTTCCT+TGG + chr1.4:12231300-12231319 None:intergenic 55.0%
GTAAGGTGGGGGTGGGAATA+AGG + chr1.4:12231263-12231282 None:intergenic 55.0%
TACCGGAACACACAGGTCTC+CGG + chr1.4:12230481-12230500 None:intergenic 55.0%
! ATTGACCACGGTAACGGTGG+AGG + chr1.4:12230373-12230392 None:intergenic 55.0%
! GCTATTGACCACGGTAACGG+TGG + chr1.4:12230376-12230395 None:intergenic 55.0%
! GTCGGTAGTGTTGTTGTCGG+AGG + chr1.4:12230241-12230260 None:intergenic 55.0%
!! GGCTGAGTCAAGGTTGGTGT+CGG + chr1.4:12230328-12230347 None:intergenic 55.0%
!! GGTTGGGTTGGGAAAGCTCA+CGG + chr1.4:12231101-12231120 None:intergenic 55.0%
!! GTTGGGTTGGGAAAGCTCAC+GGG + chr1.4:12231100-12231119 None:intergenic 55.0%
!! TGTTTTCGGGCCATTAGGCC+CGG + chr1.4:12231412-12231431 None:intergenic 55.0%
!! TTGGGTTGGGAAAGCTCACG+GGG + chr1.4:12231099-12231118 None:intergenic 55.0%
ACGGTAGGTAAGGTGGGGGT+GGG + chr1.4:12231270-12231289 None:intergenic 60.0%
ACGTGAGCTCCAGTCGCAAG+GGG + chr1.4:12231193-12231212 None:intergenic 60.0%
AGACGTGAGCTCCAGTCGCA+AGG + chr1.4:12231195-12231214 None:intergenic 60.0%
ATGACTTAGCGGCCCTGGAC+TGG - chr1.4:12230980-12230999 MS.gene32455:intron 60.0%
CAATGGCGAACCGGGCCTAA+TGG - chr1.4:12231399-12231418 MS.gene32455:CDS 60.0%
CAGCTAAGGCCCGTAGCAAG+AGG - chr1.4:12231157-12231176 MS.gene32455:intron 60.0%
CCCACGAGTCCTCTTGCTAC+GGG + chr1.4:12231169-12231188 None:intergenic 60.0%
CCCGTAGCAAGAGGACTCGT+GGG - chr1.4:12231166-12231185 MS.gene32455:intron 60.0%
CTCCGGAGACCTGTGTGTTC+CGG - chr1.4:12230476-12230495 MS.gene32455:CDS 60.0%
CTCTTCCTCCACCGTTACCG+TGG - chr1.4:12230365-12230384 MS.gene32455:CDS 60.0%
CTCTTGCTACGGGCCTTAGC+TGG + chr1.4:12231159-12231178 None:intergenic 60.0%
GACGTGAGCTCCAGTCGCAA+GGG + chr1.4:12231194-12231213 None:intergenic 60.0%
GAGGGTCAGGTGGAGATGGT+CGG - chr1.4:12231343-12231362 MS.gene32455:intron 60.0%
GCCCACGAGTCCTCTTGCTA+CGG + chr1.4:12231170-12231189 None:intergenic 60.0%
GCTCACGGGGTTCTTCATCC+TGG + chr1.4:12231086-12231105 None:intergenic 60.0%
GTTGGAGCTACGGAGGCAGA+AGG - chr1.4:12231545-12231564 MS.gene32455:CDS 60.0%
! AAGGACAGTGACGGAGGGTC+AGG - chr1.4:12231330-12231349 MS.gene32455:intron 60.0%
! CACGGTAACGGTGGAGGAAG+AGG + chr1.4:12230367-12230386 None:intergenic 60.0%
! GGGCATGAAGGTGCGAAACC+AGG - chr1.4:12231065-12231084 MS.gene32455:intron 60.0%
! GGTAACGGTGGAGGAAGAGG+CGG + chr1.4:12230364-12230383 None:intergenic 60.0%
! TTAGGCCCGGTTCGCCATTG+TGG + chr1.4:12231399-12231418 None:intergenic 60.0%
CGTGAGCTCCAGTCGCAAGG+GGG + chr1.4:12231192-12231211 None:intergenic 65.0%
GACGGAGGGTCAGGTGGAGA+TGG - chr1.4:12231339-12231358 MS.gene32455:intron 65.0%
GACGGTAGGTAAGGTGGGGG+TGG + chr1.4:12231271-12231290 None:intergenic 65.0%
GCCCGTAGCAAGAGGACTCG+TGG - chr1.4:12231165-12231184 MS.gene32455:intron 65.0%
GGAGGATGACTTAGCGGCCC+TGG - chr1.4:12230975-12230994 MS.gene32455:intron 65.0%
GGTCAGGTGGAGATGGTCGG+AGG - chr1.4:12231346-12231365 MS.gene32455:intron 65.0%
! GACAGTGACGGAGGGTCAGG+TGG - chr1.4:12231333-12231352 MS.gene32455:intron 65.0%
CTCGTGGGCCCCCTTGCGAC+TGG - chr1.4:12231181-12231200 MS.gene32455:intron 75.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 12230198 12231698 12230198 ID=MS.gene32455
chr1.4 mRNA 12230198 12231698 12230198 ID=MS.gene32455.t1;Parent=MS.gene32455
chr1.4 exon 12231399 12231698 12231399 ID=MS.gene32455.t1.exon1;Parent=MS.gene32455.t1
chr1.4 CDS 12231399 12231698 12231399 ID=cds.MS.gene32455.t1;Parent=MS.gene32455.t1
chr1.4 exon 12230198 12230920 12230198 ID=MS.gene32455.t1.exon2;Parent=MS.gene32455.t1
chr1.4 CDS 12230198 12230920 12230198 ID=cds.MS.gene32455.t1;Parent=MS.gene32455.t1
Gene Sequence

>MS.gene32455

ATGGAAGGCACAACCAACAACATTTTCCAAACCACCTTTTCCTCCGACAACAACACTACCGACAATTTCATTGTTGAGGACCTCCTTAACCTCAACAATGACGTCGACGACGACGCTACCATCATTTCCGACACCAACCTTGACTCAGCCACAATCAACTCAACCGCCTCTTCCTCCACCGTTACCGTGGTCAATAGCGTTAGCTCGTCATCATTGTCCGGGTGCGACCCTAATGTCGTTCCTGATATTGGCTGTCATAATTTTTCTGACAGCCATTTCTCCGGAGACCTGTGTGTTCCGGTATATTATATGTGTATATGCATTATCAATTATTATCTTTTTTTAAAAAATATATATAAAGCACGTAGACACTTCACATTTTCGTAACTCGTTTAATTTGACTTATTCTATTAGTTCCATGTAAATTTCTAACTCATGCACGTATAACATATAACATCACATTGCATTTGAAGATTTTGAACCCTTTTTTGCCAATAATTTCAGGAGAAAAAACAACTAGCTAGGCTAAATACAACAGAATGGGGCACATTTCACGGTTCGAAAACTAAATTTTATTGCAACATTTGTTTCAATTATTACCAAATTATTGAGTATCACAAAATAGTACACGTGAAAGCATTAGTACGACTATATGAACATAAAATGTGCATGCTTGATTAGTTATGTACAATGTAATATTATTATTACTACGTAGCAAATAATGTTTCAAGAGCATATATAGAGGAATAATTATTGTTTGTGTTGAAATTTGAAATAGGAGGATGACTTAGCGGCCCTGGACTGGATTTCCCATATTGATGAGGAATCGTTTTCAAGCGAGGATCTTCAAAAGATGCAACTGATATCGGGCATGAAGGTGCGAAACCAGGATGAAGAACCCCGTGAGCTTTCCCAACCCAACCGAAACAGTCCTATATTCAACAAGGAAGTTTTGGTTCCAGCTAAGGCCCGTAGCAAGAGGACTCGTGGGCCCCCTTGCGACTGGAGCTCACGTCTTCTTGTTTTGTCTCAAACAACACCGTCTTCTTCTGAGTCAGAACTCCTTATTCCCACCCCCACCTTACCTACCGTCACTGTTCCAAGGAAGCAGGCAAAGACTGCTCCAAGAAGAAAGGACAGTGACGGAGGGTCAGGTGGAGATGGTCGGAGGTGTCTGCATTGTATGACAGACAAGACACCACAATGGCGAACCGGGCCTAATGGCCCGAAAACATTATGCAACGCTTGTGGTGTAAGATACAAGTCGGGTCGATTGGTGCCTGAATACAGGCCTGCAGCTAGCCCTACATTTGTGCTGACCAAACACTCCAACTCGCATCGTAAAGTGTTGGAGCTACGGAGGCAGAAGGAAATGTTGCGGGCACACCAACACCAACAAAACCAGATTTTACAACATCAGAGTATGATGTTTGATATGTCCAACAATGATGATTACTTGATTCACCAACATATGGGACCTGATTTTAGGCAGATGATGTAG
Protein sequence

>MS.gene32455.t1

MEGTTNNIFQTTFSSDNNTTDNFIVEDLLNLNNDVDDDATIISDTNLDSATINSTASSSTVTVVNSVSSSSLSGCDPNVVPDIGCHNFSDSHFSGDLCVPEDDLAALDWISHIDEESFSSEDLQKMQLISGMKVRNQDEEPRELSQPNRNSPIFNKEVLVPAKARSKRTRGPPCDWSSRLLVLSQTTPSSSESELLIPTPTLPTVTVPRKQAKTAPRRKDSDGGSGGDGRRCLHCMTDKTPQWRTGPNGPKTLCNACGVRYKSGRLVPEYRPAASPTFVLTKHSNSHRKVLELRRQKEMLRAHQHQQNQILQHQSMMFDMSNNDDYLIHQHMGPDFRQMM