Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32525.t1 | XP_003616466.2 | 71.7 | 233 | 5 | 3 | 1 | 173 | 1 | 232 | 7.80E-82 | 313.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32525.t1 | O80942 | 64.3 | 70 | 19 | 2 | 11 | 80 | 7 | 70 | 6.2e-19 | 95.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32525.t1 | G7KGU7 | 71.7 | 233 | 5 | 3 | 1 | 173 | 1 | 232 | 5.7e-82 | 313.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene32525.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32525.t1 | MTR_5g080660 | 67.672 | 232 | 16 | 3 | 1 | 173 | 1 | 232 | 8.28e-104 | 297 |
| MS.gene32525.t1 | MTR_3g068095 | 64.706 | 85 | 17 | 2 | 11 | 83 | 8 | 91 | 9.57e-31 | 112 |
| MS.gene32525.t1 | MTR_3g068095 | 37.821 | 156 | 68 | 9 | 40 | 173 | 112 | 260 | 2.02e-11 | 60.8 |
| MS.gene32525.t1 | MTR_4g021270 | 71.014 | 69 | 16 | 1 | 23 | 91 | 35 | 99 | 6.29e-28 | 106 |
| MS.gene32525.t1 | MTR_5g014400 | 64.474 | 76 | 24 | 3 | 10 | 84 | 44 | 117 | 5.64e-27 | 102 |
| MS.gene32525.t1 | MTR_1g070265 | 59.701 | 67 | 26 | 1 | 41 | 106 | 29 | 95 | 1.68e-19 | 81.3 |
| MS.gene32525.t1 | MTR_2g076060 | 66.667 | 48 | 15 | 1 | 38 | 84 | 40 | 87 | 3.09e-18 | 79.0 |
| MS.gene32525.t1 | MTR_4g064873 | 73.684 | 38 | 10 | 0 | 46 | 83 | 20 | 57 | 1.70e-14 | 67.8 |
| MS.gene32525.t1 | MTR_7g063220 | 38.710 | 124 | 54 | 3 | 47 | 170 | 24 | 125 | 3.80e-14 | 66.2 |
| MS.gene32525.t1 | MTR_1g070250 | 45.833 | 72 | 32 | 2 | 43 | 107 | 34 | 105 | 3.54e-11 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32525.t1 | AT2G37740 | 64.286 | 70 | 19 | 2 | 11 | 80 | 7 | 70 | 1.48e-25 | 99.8 |
| MS.gene32525.t1 | AT4G17810 | 62.319 | 69 | 23 | 2 | 10 | 76 | 6 | 73 | 1.01e-24 | 94.7 |
| MS.gene32525.t1 | AT4G17810 | 62.319 | 69 | 23 | 2 | 10 | 76 | 6 | 73 | 1.74e-24 | 94.7 |
| MS.gene32525.t1 | AT5G06070 | 55.952 | 84 | 26 | 2 | 6 | 88 | 22 | 95 | 8.36e-23 | 90.9 |
| MS.gene32525.t1 | AT3G23130 | 76.744 | 43 | 10 | 0 | 37 | 79 | 35 | 77 | 2.33e-18 | 79.0 |
| MS.gene32525.t1 | AT2G42410 | 78.378 | 37 | 8 | 0 | 44 | 80 | 44 | 80 | 5.83e-17 | 75.5 |
| MS.gene32525.t1 | AT2G42410 | 78.378 | 37 | 8 | 0 | 44 | 80 | 44 | 80 | 5.83e-17 | 75.5 |
| MS.gene32525.t1 | AT2G42410 | 78.378 | 37 | 8 | 0 | 44 | 80 | 70 | 106 | 6.04e-17 | 75.9 |
| MS.gene32525.t1 | AT3G09290 | 30.769 | 156 | 67 | 4 | 39 | 169 | 25 | 164 | 1.42e-14 | 68.2 |
| MS.gene32525.t1 | AT3G53820 | 50.725 | 69 | 30 | 3 | 40 | 106 | 14 | 80 | 1.30e-13 | 65.1 |
Find 30 sgRNAs with CRISPR-Local
Find 47 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTCTGATCATTACTGGTTT+TGG | 0.234168 | 5.1:+62695343 | MS.gene32525:CDS |
| AAATGATCATCATAGATTTC+TGG | 0.240116 | 5.1:+62695938 | MS.gene32525:CDS |
| GATCTTACTCTGATCATTAC+TGG | 0.268968 | 5.1:+62695337 | MS.gene32525:CDS |
| ACTATTCCTAGTGAGGAAAA+AGG | 0.342937 | 5.1:+62695843 | MS.gene32525:CDS |
| AGTTTGTCTTTAGGAATGTT+TGG | 0.376046 | 5.1:+62695807 | MS.gene32525:intron |
| TTAGATCTTGAACTCAGGTT+GGG | 0.396208 | 5.1:+62696014 | MS.gene32525:CDS |
| TTTAGGTCTGCTCAAGCTCT+TGG | 0.423987 | 5.1:+62695501 | MS.gene32525:CDS |
| CAACCTTCAGAAATTGTACT+AGG | 0.453879 | 5.1:+62695969 | MS.gene32525:CDS |
| TTCAAGCACCAATTGAATCA+TGG | 0.457719 | 5.1:+62695382 | MS.gene32525:CDS |
| AATTGAATCATGGGAAGAAA+AGG | 0.467520 | 5.1:+62695392 | MS.gene32525:CDS |
| ATCAAATCATTGTTCTTCTG+AGG | 0.467552 | 5.1:-62695579 | None:intergenic |
| TTTAGATCTTGAACTCAGGT+TGG | 0.474254 | 5.1:+62696013 | MS.gene32525:CDS |
| TCAAGCACCAATTGAATCAT+GGG | 0.500236 | 5.1:+62695383 | MS.gene32525:CDS |
| AAGATTTAGATCTTGAACTC+AGG | 0.503939 | 5.1:+62696009 | MS.gene32525:CDS |
| AAGTTACATGAATAAAATCT+TGG | 0.505499 | 5.1:-62695465 | None:intergenic |
| AGGTCTGCTCAAGCTCTTGG+TGG | 0.535050 | 5.1:+62695504 | MS.gene32525:CDS |
| TAGATCTTGAACTCAGGTTG+GGG | 0.538027 | 5.1:+62696015 | MS.gene32525:CDS |
| TCAAATCATTGTTCTTCTGA+GGG | 0.542057 | 5.1:-62695578 | None:intergenic |
| GTGGTCACATGAATATTCAT+AGG | 0.543934 | 5.1:+62695523 | MS.gene32525:CDS |
| GCAGCTAGGAATATATTGAA+TGG | 0.562026 | 5.1:+62695429 | MS.gene32525:CDS |
| ATTGTACTAGGACTTGATCA+TGG | 0.568821 | 5.1:+62695981 | MS.gene32525:CDS |
| TTACATGAATAAAATCTTGG+TGG | 0.571433 | 5.1:-62695462 | None:intergenic |
| AGTCCTAGTACAATTTCTGA+AGG | 0.591874 | 5.1:-62695972 | None:intergenic |
| TTCATAGGAGAGATAGAGCT+AGG | 0.597256 | 5.1:+62695538 | MS.gene32525:CDS |
| ATCATCAACTATTCCTAGTG+AGG | 0.599143 | 5.1:+62695836 | MS.gene32525:CDS |
| ACTAGGACTTGATCATGGAA+TGG | 0.618438 | 5.1:+62695986 | MS.gene32525:CDS |
| GATTTGATCAACTCATCACT+AGG | 0.628308 | 5.1:+62695594 | MS.gene32525:CDS |
| GATCATTTGAACATGTCTTG+AGG | 0.638691 | 5.1:-62695923 | None:intergenic |
| CATGTCTTGAGGAACACAGA+AGG | 0.671603 | 5.1:-62695912 | None:intergenic |
| ATATATTGAATGGTAGCATG+TGG | 0.672779 | 5.1:+62695439 | MS.gene32525:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TGATAATTACAATCTATTTT+TGG | - | chr5.1:62695648-62695667 | None:intergenic | 15.0% |
| !! | AAGTTACATGAATAAAATCT+TGG | - | chr5.1:62695468-62695487 | None:intergenic | 20.0% |
| !! | CTTCTTATAGTTGTAATTAA+GGG | - | chr5.1:62695702-62695721 | None:intergenic | 20.0% |
| !! | TCTTCTTATAGTTGTAATTA+AGG | - | chr5.1:62695703-62695722 | None:intergenic | 20.0% |
| ! | AAATGATCATCATAGATTTC+TGG | + | chr5.1:62695938-62695957 | MS.gene32525:CDS | 25.0% |
| ! | ATAGTTGAGATAATTCTAGA+AGG | - | chr5.1:62695735-62695754 | None:intergenic | 25.0% |
| ! | TTACATGAATAAAATCTTGG+TGG | - | chr5.1:62695465-62695484 | None:intergenic | 25.0% |
| !! | ACTTTTGTAAAAGAGAGTTT+AGG | + | chr5.1:62695484-62695503 | MS.gene32525:CDS | 25.0% |
| !! | TGCTAAAGAAGACAAATTTT+TGG | + | chr5.1:62695761-62695780 | MS.gene32525:intron | 25.0% |
| AAGATTTAGATCTTGAACTC+AGG | + | chr5.1:62696009-62696028 | MS.gene32525:CDS | 30.0% | |
| AATTGAATCATGGGAAGAAA+AGG | + | chr5.1:62695392-62695411 | MS.gene32525:CDS | 30.0% | |
| ATATATTGAATGGTAGCATG+TGG | + | chr5.1:62695439-62695458 | MS.gene32525:CDS | 30.0% | |
| ATCAAATCATTGTTCTTCTG+AGG | - | chr5.1:62695582-62695601 | None:intergenic | 30.0% | |
| GTTGAAACAAGTTTGTCTTT+AGG | + | chr5.1:62695798-62695817 | MS.gene32525:intron | 30.0% | |
| TCAAATCATTGTTCTTCTGA+GGG | - | chr5.1:62695581-62695600 | None:intergenic | 30.0% | |
| ! | AGTTTGTCTTTAGGAATGTT+TGG | + | chr5.1:62695807-62695826 | MS.gene32525:intron | 30.0% |
| ! | TTGTAATTAAGGGTTTTGCA+GGG | - | chr5.1:62695692-62695711 | None:intergenic | 30.0% |
| ! | TTTTCTTCCCATGATTCAAT+TGG | - | chr5.1:62695393-62695412 | None:intergenic | 30.0% |
| ACTATTCCTAGTGAGGAAAA+AGG | + | chr5.1:62695843-62695862 | MS.gene32525:CDS | 35.0% | |
| AGTCCTAGTACAATTTCTGA+AGG | - | chr5.1:62695975-62695994 | None:intergenic | 35.0% | |
| ATCATCAACTATTCCTAGTG+AGG | + | chr5.1:62695836-62695855 | MS.gene32525:CDS | 35.0% | |
| ATTGTACTAGGACTTGATCA+TGG | + | chr5.1:62695981-62696000 | MS.gene32525:CDS | 35.0% | |
| CAACCTTCAGAAATTGTACT+AGG | + | chr5.1:62695969-62695988 | MS.gene32525:CDS | 35.0% | |
| GATCATTTGAACATGTCTTG+AGG | - | chr5.1:62695926-62695945 | None:intergenic | 35.0% | |
| GATCTTACTCTGATCATTAC+TGG | + | chr5.1:62695337-62695356 | MS.gene32525:CDS | 35.0% | |
| GATTTGATCAACTCATCACT+AGG | + | chr5.1:62695594-62695613 | MS.gene32525:CDS | 35.0% | |
| GCAGCTAGGAATATATTGAA+TGG | + | chr5.1:62695429-62695448 | MS.gene32525:CDS | 35.0% | |
| GTGGTCACATGAATATTCAT+AGG | + | chr5.1:62695523-62695542 | MS.gene32525:CDS | 35.0% | |
| TCAAGCACCAATTGAATCAT+GGG | + | chr5.1:62695383-62695402 | MS.gene32525:CDS | 35.0% | |
| TTAGATCTTGAACTCAGGTT+GGG | + | chr5.1:62696014-62696033 | MS.gene32525:CDS | 35.0% | |
| TTCAAGCACCAATTGAATCA+TGG | + | chr5.1:62695382-62695401 | MS.gene32525:CDS | 35.0% | |
| TTTAGATCTTGAACTCAGGT+TGG | + | chr5.1:62696013-62696032 | MS.gene32525:CDS | 35.0% | |
| ! | AGATAATTCTAGAAGGTGAG+AGG | - | chr5.1:62695728-62695747 | None:intergenic | 35.0% |
| ! | ATGATACCTTTTTCCTCACT+AGG | - | chr5.1:62695852-62695871 | None:intergenic | 35.0% |
| ! | GATAATTCTAGAAGGTGAGA+GGG | - | chr5.1:62695727-62695746 | None:intergenic | 35.0% |
| ! | GTTGTAATTAAGGGTTTTGC+AGG | - | chr5.1:62695693-62695712 | None:intergenic | 35.0% |
| !!! | ACTCTGATCATTACTGGTTT+TGG | + | chr5.1:62695343-62695362 | MS.gene32525:CDS | 35.0% |
| ACTAGGACTTGATCATGGAA+TGG | + | chr5.1:62695986-62696005 | MS.gene32525:CDS | 40.0% | |
| TTCATAGGAGAGATAGAGCT+AGG | + | chr5.1:62695538-62695557 | MS.gene32525:CDS | 40.0% | |
| ! | TAGATCTTGAACTCAGGTTG+GGG | + | chr5.1:62696015-62696034 | MS.gene32525:CDS | 40.0% |
| !! | GTGAGGAAAAAGGTATCATG+AGG | + | chr5.1:62695853-62695872 | MS.gene32525:CDS | 40.0% |
| !!! | ACTGGTTTTGGATGAAGAGA+AGG | + | chr5.1:62695355-62695374 | MS.gene32525:CDS | 40.0% |
| CATGTCTTGAGGAACACAGA+AGG | - | chr5.1:62695915-62695934 | None:intergenic | 45.0% | |
| TTTAGGTCTGCTCAAGCTCT+TGG | + | chr5.1:62695501-62695520 | MS.gene32525:CDS | 45.0% | |
| ! | CTTTTGCAGAAGATGCAGCT+AGG | + | chr5.1:62695415-62695434 | MS.gene32525:CDS | 45.0% |
| ! | GGTTTTGCAGGGCTAGAACA+AGG | - | chr5.1:62695681-62695700 | None:intergenic | 50.0% |
| AGGTCTGCTCAAGCTCTTGG+TGG | + | chr5.1:62695504-62695523 | MS.gene32525:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 62695330 | 62696058 | 62695330 | ID=MS.gene32525 |
| chr5.1 | mRNA | 62695330 | 62696058 | 62695330 | ID=MS.gene32525.t1;Parent=MS.gene32525 |
| chr5.1 | exon | 62695330 | 62695615 | 62695330 | ID=MS.gene32525.t1.exon1;Parent=MS.gene32525.t1 |
| chr5.1 | CDS | 62695330 | 62695615 | 62695330 | ID=cds.MS.gene32525.t1;Parent=MS.gene32525.t1 |
| chr5.1 | exon | 62695820 | 62696058 | 62695820 | ID=MS.gene32525.t1.exon2;Parent=MS.gene32525.t1 |
| chr5.1 | CDS | 62695820 | 62696058 | 62695820 | ID=cds.MS.gene32525.t1;Parent=MS.gene32525.t1 |
| Gene Sequence |
| Protein sequence |
