AlfalfaGEDB | MS.gene32577

M. sativa cultivar XinJiangDaYe / MS.gene32577

KEGG: -
GO: GO:0000166 GO:0003674 GO:0003824 GO:0004672 GO:0005488 GO:0005524 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008144 GO:0008150 GO:0008152 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017076 GO:0019538 GO:0030554 GO:0032553 GO:0032555 GO:0032559 GO:0035639 GO:0036094 GO:0036211 GO:0043167 GO:0043168 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0071704 GO:0097159 GO:0097367 GO:0140096 GO:1901265 GO:1901363 GO:1901564
PFAM: PF00069.28

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene32577.t1	XP_027345640.1	85	60	8	1	1	60	210	268	1.80E-19	105.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene32577.t1	P53681	81.7	60	10	1	1	60	225	283	4.9e-21	101.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene32577.t1	G7JMG6	85.0	60	8	1	1	60	214	272	1.3e-19	105.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene32577.t1	MTR_4g086660	89.474	57	5	1	1	57	214	269	7.44e-29	108
MS.gene32577.t1	MTR_8g079790	82.456	57	9	1	1	57	205	260	5.59e-26	100
MS.gene32577.t1	MTR_5g017830	75.439	57	13	1	1	57	211	266	4.72e-24	94.7
MS.gene32577.t1	MTR_7g118020	73.684	57	14	1	1	57	221	276	7.97e-24	94.4
MS.gene32577.t1	MTR_3g092230	70.000	60	17	1	1	60	215	273	2.99e-23	92.8
MS.gene32577.t1	MTR_7g089480	73.684	57	14	1	1	57	218	273	3.09e-23	92.4
MS.gene32577.t1	MTR_5g022030	45.349	86	34	3	1	85	173	246	6.56e-15	68.9
MS.gene32577.t1	MTR_5g022030	45.349	86	34	3	1	85	173	246	8.84e-15	68.6
MS.gene32577.t1	MTR_5g092815	57.407	54	22	1	4	57	167	219	1.96e-14	67.4
MS.gene32577.t1	MTR_3g051770	53.704	54	24	1	4	57	160	212	1.45e-12	62.4
MS.gene32577.t1	MTR_1g026075	50.000	54	26	1	7	60	153	205	2.05e-12	60.8
MS.gene32577.t1	MTR_5g092810	56.863	51	21	1	8	58	172	221	2.33e-12	61.6
MS.gene32577.t1	MTR_1g026190	54.717	53	21	1	8	57	158	210	3.47e-12	61.2
MS.gene32577.t1	MTR_7g068710	58.000	50	20	1	8	57	136	184	4.26e-12	60.8
MS.gene32577.t1	MTR_7g091890	58.000	50	20	1	8	57	138	186	5.76e-12	60.5
MS.gene32577.t1	MTR_7g091890	58.000	50	20	1	8	57	138	186	6.30e-12	60.5
MS.gene32577.t1	MTR_4g107490	58.000	50	20	1	8	57	153	201	3.18e-11	58.5
MS.gene32577.t1	MTR_1g052530	54.000	50	22	1	8	57	132	180	5.41e-11	57.8
MS.gene32577.t1	MTR_1g052530	54.000	50	22	1	8	57	132	180	5.80e-11	57.8
MS.gene32577.t1	MTR_8g095440	42.308	78	33	2	8	85	110	175	6.86e-11	57.4
MS.gene32577.t1	MTR_8g095440	41.772	79	34	2	7	85	109	175	8.76e-11	57.0
MS.gene32577.t1	MTR_5g009830	52.727	55	25	1	8	62	147	200	9.40e-11	57.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene32577.t1	AT3G50530	80.702	57	10	1	1	57	225	280	4.78e-26	100
MS.gene32577.t1	AT3G50530	80.702	57	10	1	1	57	225	280	4.90e-26	100
MS.gene32577.t1	AT3G19100	80.702	57	10	1	1	57	221	276	1.51e-25	99.4
MS.gene32577.t1	AT1G49580	84.000	50	8	0	8	57	233	282	1.94e-24	95.9
MS.gene32577.t1	AT2G46700	75.439	57	13	1	1	57	220	275	4.42e-24	95.1
MS.gene32577.t1	AT2G46700	75.439	57	13	1	1	57	39	94	6.10e-24	94.0
MS.gene32577.t1	AT2G46700	75.439	57	13	1	1	57	39	94	6.10e-24	94.0
MS.gene32577.t1	AT2G41140	75.000	60	14	1	1	60	200	258	7.04e-24	94.4
MS.gene32577.t1	AT3G56760	71.667	60	16	1	1	60	201	259	1.96e-22	90.5
MS.gene32577.t1	AT3G49370	68.519	54	17	0	4	57	221	274	2.61e-19	81.6
MS.gene32577.t1	AT5G24430	69.811	53	16	0	8	60	226	278	2.69e-19	81.3
MS.gene32577.t1	AT2G17890	47.674	86	32	3	1	85	182	255	8.46e-16	71.6
MS.gene32577.t1	AT5G66210	58.621	58	22	2	1	57	136	192	4.18e-15	69.3
MS.gene32577.t1	AT5G66210	58.621	58	22	2	1	57	136	192	4.65e-15	69.3
MS.gene32577.t1	AT5G66210	58.621	58	22	2	1	57	136	192	4.65e-15	69.3
MS.gene32577.t1	AT5G66210	58.621	58	22	2	1	57	136	192	4.81e-15	69.3
MS.gene32577.t1	AT5G66210	58.621	58	22	2	1	57	136	192	5.43e-15	69.3
MS.gene32577.t1	AT5G66210	58.621	58	22	2	1	57	136	192	5.43e-15	69.3
MS.gene32577.t1	AT4G36070	44.186	86	35	3	1	85	145	218	1.84e-14	67.8
MS.gene32577.t1	AT4G36070	44.186	86	35	3	1	85	145	218	1.85e-14	67.8
MS.gene32577.t1	AT5G12480	62.000	50	18	1	8	57	139	187	1.76e-13	64.7
MS.gene32577.t1	AT5G19450	62.000	50	18	1	8	57	137	185	3.44e-13	63.9
MS.gene32577.t1	AT5G19450	62.000	50	18	1	8	57	137	185	3.44e-13	63.9
MS.gene32577.t1	AT1G76040	58.000	50	20	1	8	57	192	240	5.80e-13	63.5
MS.gene32577.t1	AT1G76040	58.000	50	20	1	8	57	192	240	5.97e-13	63.5
MS.gene32577.t1	AT1G50700	60.000	50	19	1	8	57	153	201	8.93e-13	62.8
MS.gene32577.t1	AT1G50700	60.000	50	19	1	8	57	153	201	9.01e-13	62.8
MS.gene32577.t1	AT1G50700	60.000	50	19	1	8	57	153	201	1.01e-12	62.8
MS.gene32577.t1	AT3G20410	56.000	50	21	1	8	57	171	219	3.47e-12	61.2
MS.gene32577.t1	AT3G20410	56.000	50	21	1	8	57	171	219	3.47e-12	61.2
MS.gene32577.t1	AT5G23580	46.154	78	30	2	8	85	102	167	4.34e-12	60.8
MS.gene32577.t1	AT2G31500	58.000	50	20	1	8	57	146	194	5.85e-12	60.5
MS.gene32577.t1	AT4G04720	56.000	50	21	1	8	57	160	208	5.97e-12	60.5
MS.gene32577.t1	AT4G04720	56.000	50	21	1	8	57	160	208	5.97e-12	60.5
MS.gene32577.t1	AT2G41860	56.000	50	21	1	8	57	134	182	8.56e-12	60.1
MS.gene32577.t1	AT2G41860	56.000	50	21	1	8	57	134	182	8.56e-12	60.1
MS.gene32577.t1	AT3G51850	56.000	50	21	1	8	57	134	182	1.29e-11	59.7
MS.gene32577.t1	AT3G51850	56.000	50	21	1	8	57	134	182	1.41e-11	59.3
MS.gene32577.t1	AT1G61950	54.000	50	22	1	8	57	178	226	1.57e-11	59.3
MS.gene32577.t1	AT3G51850	56.000	50	21	1	8	57	134	182	1.63e-11	59.3
MS.gene32577.t1	AT4G21940	54.000	50	22	1	8	57	182	230	2.14e-11	58.9
MS.gene32577.t1	AT3G57530	54.717	53	23	1	8	60	143	194	2.17e-11	58.9
MS.gene32577.t1	AT4G21940	54.000	50	22	1	8	57	182	230	2.37e-11	58.9
MS.gene32577.t1	AT4G21940	54.000	50	22	1	8	57	182	230	2.37e-11	58.9
MS.gene32577.t1	AT3G57530	54.717	53	23	1	8	60	143	194	2.43e-11	58.9
MS.gene32577.t1	AT4G04740	54.000	50	22	1	8	57	149	197	2.63e-11	58.5
MS.gene32577.t1	AT4G04740	54.000	50	22	1	8	57	149	197	2.89e-11	58.5
MS.gene32577.t1	AT4G04740	54.000	50	22	1	8	57	149	197	2.89e-11	58.5
MS.gene32577.t1	AT4G04740	54.000	50	22	1	8	57	149	197	2.89e-11	58.5
MS.gene32577.t1	AT4G04740	54.000	50	22	1	8	57	149	197	2.89e-11	58.5
MS.gene32577.t1	AT4G04740	54.000	50	22	1	8	57	149	197	2.89e-11	58.5
MS.gene32577.t1	AT4G04740	54.000	50	22	1	8	57	149	197	2.89e-11	58.5

Find 19 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCAGATGCGGTACCGATAGA+AGG	0.362731	8.3:+46192784	MS.gene32577:CDS
TCAACAACAGACTGAAACTT+TGG	0.406207	8.3:-46192753	None:intergenic
GACGTTTCAGGTTATGTGAA+GGG	0.515873	8.3:+46192275	MS.gene32577:intron
TATTTGTACCATGACAGCCT+TGG	0.549299	8.3:-46192554	None:intergenic
AGATGATGCCAAGGCTGTCA+TGG	0.554908	8.3:+46192546	MS.gene32577:CDS
CATGATCCATGCCCTTCTAT+CGG	0.563859	8.3:-46192796	None:intergenic
CGACGTTTCAGGTTATGTGA+AGG	0.570325	8.3:+46192274	MS.gene32577:intron
AAAGTTTCAGTCTGTTGTTG+AGG	0.571170	8.3:+46192755	MS.gene32577:CDS
CAGATGCGGTACCGATAGAA+GGG	0.574895	8.3:+46192785	MS.gene32577:CDS
ACGTTTCAGGTTATGTGAAG+GGG	0.578083	8.3:+46192276	MS.gene32577:intron
TGAAACTTTGGTGTAAGCAT+AGG	0.589679	8.3:-46192741	None:intergenic
ATACTCAGAAGATGATGCCA+AGG	0.618920	8.3:+46192537	MS.gene32577:CDS
GCGGTACCGATAGAAGGGCA+TGG	0.625842	8.3:+46192790	MS.gene32577:CDS
CGTTTCAGGTTATGTGAAGG+GGG	0.632420	8.3:+46192277	MS.gene32577:intron
GTTGAGGATAGCAGCAGATG+CGG	0.641502	8.3:+46192771	MS.gene32577:CDS
TCATGTTTGTGCAGCAGATG+CGG	0.646291	8.3:+46192814	MS.gene32577:CDS
TATTAGACATGATACTGTCA+AGG	0.648573	8.3:+46192305	MS.gene32577:CDS
GCACAGAGATCTTAAGCCTG+AGG	0.658568	8.3:+46192615	MS.gene32577:CDS
AAATGTATGTAGTGACACCA+AGG	0.670047	8.3:+46192153	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATCATATTTAAATATCTTA+GGG	+	chr8.3:46192696-46192715	MS.gene32577:intron	10.0%
!!	TAATCATATTTAAATATCTT+AGG	+	chr8.3:46192695-46192714	MS.gene32577:intron	10.0%
!	TTAAACCTAATAAATCACCT+TGG	-	chr8.3:46192173-46192192	None:intergenic	25.0%
	ATGATTTATTGCATCTTCAG+AGG	+	chr8.3:46192508-46192527	MS.gene32577:intron	30.0%
	TATTAGACATGATACTGTCA+AGG	+	chr8.3:46192305-46192324	MS.gene32577:CDS	30.0%
	TCTTTCATTTGTCTTTCAGA+TGG	+	chr8.3:46192647-46192666	MS.gene32577:intron	30.0%
	TGAAGATGCAATAAATCATG+TGG	-	chr8.3:46192507-46192526	None:intergenic	30.0%
	AAAGTTTCAGTCTGTTGTTG+AGG	+	chr8.3:46192755-46192774	MS.gene32577:CDS	35.0%
	ATTTATTGCATCTTCAGAGG+AGG	+	chr8.3:46192511-46192530	MS.gene32577:intron	35.0%
	TAAATCATGTGGCTAGTAAG+TGG	-	chr8.3:46192496-46192515	None:intergenic	35.0%
	TCAACAACAGACTGAAACTT+TGG	-	chr8.3:46192756-46192775	None:intergenic	35.0%
	TGACACCAAGGTGATTTATT+AGG	+	chr8.3:46192165-46192184	MS.gene32577:intron	35.0%
	TTTATTGCATCTTCAGAGGA+GGG	+	chr8.3:46192512-46192531	MS.gene32577:intron	35.0%
!	ATTTTGTCATCTTCAGAGTG+TGG	+	chr8.3:46192591-46192610	MS.gene32577:CDS	35.0%
!	TGAAACTTTGGTGTAAGCAT+AGG	-	chr8.3:46192744-46192763	None:intergenic	35.0%
	ATACTCAGAAGATGATGCCA+AGG	+	chr8.3:46192537-46192556	MS.gene32577:CDS	40.0%
	CTGAATACTATCGACGTTTC+AGG	+	chr8.3:46192263-46192282	MS.gene32577:intron	40.0%
	TATTTGTACCATGACAGCCT+TGG	-	chr8.3:46192557-46192576	None:intergenic	40.0%
!!	ACGTTTCAGGTTATGTGAAG+GGG	+	chr8.3:46192276-46192295	MS.gene32577:intron	40.0%
!!	GACGTTTCAGGTTATGTGAA+GGG	+	chr8.3:46192275-46192294	MS.gene32577:intron	40.0%
	CATGATCCATGCCCTTCTAT+CGG	-	chr8.3:46192799-46192818	None:intergenic	45.0%
	GCATGAAATAGAGCCGCTAT+CGG	+	chr8.3:46192348-46192367	MS.gene32577:intron	45.0%
	TCATGTTTGTGCAGCAGATG+CGG	+	chr8.3:46192814-46192833	MS.gene32577:CDS	45.0%
	TGAAAGAATCTGCTCACCTC+AGG	-	chr8.3:46192634-46192653	None:intergenic	45.0%
	TTGAAATAACGCCGCTAACG+CGG	-	chr8.3:46192442-46192461	None:intergenic	45.0%
!!	CGACGTTTCAGGTTATGTGA+AGG	+	chr8.3:46192274-46192293	MS.gene32577:intron	45.0%
!!	CGTTTCAGGTTATGTGAAGG+GGG	+	chr8.3:46192277-46192296	MS.gene32577:intron	45.0%
	AGATGATGCCAAGGCTGTCA+TGG	+	chr8.3:46192546-46192565	MS.gene32577:CDS	50.0%
	CATGTGGCTAGTAAGTGGCA+AGG	-	chr8.3:46192491-46192510	None:intergenic	50.0%
	GCACAGAGATCTTAAGCCTG+AGG	+	chr8.3:46192615-46192634	MS.gene32577:CDS	50.0%
	GTTGAGGATAGCAGCAGATG+CGG	+	chr8.3:46192771-46192790	MS.gene32577:CDS	50.0%
!!	CAGATGCGGTACCGATAGAA+GGG	+	chr8.3:46192785-46192804	MS.gene32577:CDS	50.0%
!!	GCAGATGCGGTACCGATAGA+AGG	+	chr8.3:46192784-46192803	MS.gene32577:CDS	55.0%
!!	TGCTCACTTCTGGGGTGAGA+CGG	-	chr8.3:46192406-46192425	None:intergenic	55.0%
	ATGGCCGCTGCTCACTTCTG+GGG	-	chr8.3:46192414-46192433	None:intergenic	60.0%
	CTCACCCCAGAAGTGAGCAG+CGG	+	chr8.3:46192407-46192426	MS.gene32577:intron	60.0%
	GATGGCCGCTGCTCACTTCT+GGG	-	chr8.3:46192415-46192434	None:intergenic	60.0%
	GGCCATCATAGCCGCGTTAG+CGG	+	chr8.3:46192428-46192447	MS.gene32577:intron	60.0%
	TGATGGCCGCTGCTCACTTC+TGG	-	chr8.3:46192416-46192435	None:intergenic	60.0%
	TGGGGTGAGACGGTGCATGT+GGG	-	chr8.3:46192396-46192415	None:intergenic	60.0%
!!	GCGGTACCGATAGAAGGGCA+TGG	+	chr8.3:46192790-46192809	MS.gene32577:CDS	60.0%
	CATGTGGGGGAGGTCACCCA+CGG	-	chr8.3:46192381-46192400	None:intergenic	65.0%
	CGCCGCTAACGCGGCTATGA+TGG	-	chr8.3:46192433-46192452	None:intergenic	65.0%
	CTGGGGTGAGACGGTGCATG+TGG	-	chr8.3:46192397-46192416	None:intergenic	65.0%
	GGGGTGAGACGGTGCATGTG+GGG	-	chr8.3:46192395-46192414	None:intergenic	65.0%
	GGGTGAGACGGTGCATGTGG+GGG	-	chr8.3:46192394-46192413	None:intergenic	65.0%
	TGAGACGGTGCATGTGGGGG+AGG	-	chr8.3:46192391-46192410	None:intergenic	65.0%
	CGCTATCGGCGCAGTGCCGT+GGG	+	chr8.3:46192362-46192381	MS.gene32577:intron	70.0%
!	CCACGGCACTGCGCCGATAG+CGG	-	chr8.3:46192364-46192383	None:intergenic	70.0%
	CCGCTATCGGCGCAGTGCCG+TGG	+	chr8.3:46192361-46192380	MS.gene32577:intron	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.3	gene	46192155	46192845	46192155	ID=MS.gene32577
chr8.3	mRNA	46192155	46192845	46192155	ID=MS.gene32577.t1;Parent=MS.gene32577
chr8.3	exon	46192155	46192174	46192155	ID=MS.gene32577.t1.exon1;Parent=MS.gene32577.t1
chr8.3	CDS	46192155	46192174	46192155	ID=cds.MS.gene32577.t1;Parent=MS.gene32577.t1
chr8.3	exon	46192285	46192326	46192285	ID=MS.gene32577.t1.exon2;Parent=MS.gene32577.t1
chr8.3	CDS	46192285	46192326	46192285	ID=cds.MS.gene32577.t1;Parent=MS.gene32577.t1
chr8.3	exon	46192528	46192636	46192528	ID=MS.gene32577.t1.exon3;Parent=MS.gene32577.t1
chr8.3	CDS	46192528	46192636	46192528	ID=cds.MS.gene32577.t1;Parent=MS.gene32577.t1
chr8.3	exon	46192717	46192845	46192717	ID=MS.gene32577.t1.exon4;Parent=MS.gene32577.t1
chr8.3	CDS	46192717	46192845	46192717	ID=cds.MS.gene32577.t1;Parent=MS.gene32577.t1

Gene Sequence

>MS.gene32577

ATGTATGTAGTGACACCAAGGTGATTTATTAGGTTTAACATTTATTTGTTGATTTGAATGATTGCTACTTGTGAGAATTTTTTGTTTATATAGCATATTCATAATTAACTGAATACTATCGACGTTTCAGGTTATGTGAAGGGGGAGAGCTATTAGACATGATACTGTCAAGGTAATTATTATGTTGTCAATAGCATGAAATAGAGCCGCTATCGGCGCAGTGCCGTGGGTGACCTCCCCCACATGCACCGTCTCACCCCAGAAGTGAGCAGCGGCCATCATAGCCGCGTTAGCGGCGTTATTTCAATAATAAAATTTGACTTAGTAATTGCACCTTGCCACTTACTAGCCACATGATTTATTGCATCTTCAGAGGAGGGAAATACTCAGAAGATGATGCCAAGGCTGTCATGGTACAAATACTAAATGTTGTTGCATTTTGTCATCTTCAGAGTGTGGTGCACAGAGATCTTAAGCCTGAGGTGAGCAGATTCTTTCATTTGTCTTTCAGATGGTTAATGTCTTAGAGTCTGCTATAGTTAATCATATTTAAATATCTTAGGGTCATATTTCTCTGTTCTTGATGCCTATGCTTACACCAAAGTTTCAGTCTGTTGTTGAGGATAGCAGCAGATGCGGTACCGATAGAAGGGCATGGATCATGTTTGTGCAGCAGATGCGGTACCGATAG

Protein sequence

>MS.gene32577.t1

MYVVTPRLCEGGELLDMILSRGGKYSEDDAKAVMVQILNVVAFCHLQSVVHRDLKPEGHISLFLMPMLTPKFQSVVEDSSRCGTDRRAWIMFVQQMRYR