AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene32581

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32581.t1 MTR_1g035400 88.136 531 53 4 1 521 48 578 0.0 933
MS.gene32581.t1 MTR_1g035420 83.752 517 83 1 6 521 143 659 0.0 888
MS.gene32581.t1 MTR_8g070070 66.450 462 151 3 54 511 299 760 0.0 650
MS.gene32581.t1 MTR_8g071030 55.415 554 202 5 3 511 187 740 0.0 620
MS.gene32581.t1 MTR_8g070075 52.817 568 206 7 6 511 186 753 0.0 602
MS.gene32581.t1 MTR_6g044690 55.172 522 195 10 15 508 54 564 0.0 555
MS.gene32581.t1 MTR_0376s0030 50.936 534 222 10 16 521 57 578 9.59e-178 513
MS.gene32581.t1 MTR_6g043960 50.389 514 195 11 23 508 1 482 8.94e-165 476
MS.gene32581.t1 MTR_2g054460 47.519 524 183 13 15 508 3 464 6.43e-150 437
MS.gene32581.t1 MTR_7g088370 39.879 494 275 10 21 508 203 680 2.30e-116 359
MS.gene32581.t1 MTR_8g042750 38.235 510 277 15 21 509 167 659 1.35e-101 320
MS.gene32581.t1 MTR_3g011440 36.399 511 283 13 21 507 141 633 2.30e-101 319
MS.gene32581.t1 MTR_5g013420 36.174 528 300 14 3 516 563 1067 2.00e-95 313
MS.gene32581.t1 MTR_5g013420 36.174 528 300 14 3 516 563 1067 2.19e-95 313
MS.gene32581.t1 MTR_4g010830 34.522 533 311 14 3 520 455 964 8.30e-89 293
MS.gene32581.t1 MTR_4g010550 34.519 478 255 16 47 516 15 442 3.46e-80 258
MS.gene32581.t1 MTR_7g084090 36.401 489 278 13 52 516 258 737 4.64e-80 265
MS.gene32581.t1 MTR_6g061270 36.214 486 282 12 52 516 196 674 3.70e-79 261
MS.gene32581.t1 MTR_6g061200 35.010 477 271 12 52 516 196 645 3.11e-73 245
MS.gene32581.t1 MTR_7g084090 38.827 358 203 8 52 397 258 611 3.03e-67 229
MS.gene32581.t1 MTR_1g048950 31.470 483 264 15 50 506 66 507 5.99e-53 188
MS.gene32581.t1 MTR_7g445710 29.132 484 246 15 55 514 110 520 1.06e-45 168
MS.gene32581.t1 MTR_7g445530 28.931 477 242 15 55 507 110 513 4.49e-45 166
MS.gene32581.t1 MTR_7g450730 29.451 455 233 14 55 507 107 475 4.62e-45 166
MS.gene32581.t1 MTR_7g052480 31.922 307 196 7 215 511 227 530 6.71e-42 158
MS.gene32581.t1 MTR_6g044000 42.500 120 51 1 23 124 1 120 1.61e-26 106
MS.gene32581.t1 MTR_6g044000 60.377 53 21 0 456 508 117 169 5.63e-15 73.6
MS.gene32581.t1 MTR_1g080340 26.059 307 156 11 258 508 269 560 6.92e-19 90.5
MS.gene32581.t1 MTR_1g069570 25.163 306 159 10 258 508 430 720 8.81e-19 90.5
MS.gene32581.t1 MTR_8g078490 26.829 287 155 12 258 506 960 1229 9.34e-17 84.3
MS.gene32581.t1 MTR_5g098680 33.974 156 90 3 80 229 267 415 2.43e-16 82.4
MS.gene32581.t1 MTR_5g018850 33.540 161 85 4 325 479 1312 1456 4.51e-16 82.4
MS.gene32581.t1 MTR_4g132610 27.660 188 114 3 325 506 852 1023 9.91e-16 80.9
MS.gene32581.t1 MTR_8g078505 29.032 186 112 3 325 506 346 515 2.72e-15 79.0
MS.gene32581.t1 MTR_8g102580 31.410 156 94 3 80 229 261 409 9.96e-15 77.4
MS.gene32581.t1 MTR_6g027690 28.641 206 120 7 304 507 217 397 9.52e-13 70.5
MS.gene32581.t1 MTR_3g114910 30.000 150 94 3 80 229 187 325 2.58e-12 68.9
MS.gene32581.t1 MTR_7g445810 33.121 157 89 5 55 208 123 266 2.50e-11 64.7
MS.gene32581.t1 MTR_7g445600 32.540 126 74 3 55 180 106 220 2.66e-11 65.5
MS.gene32581.t1 MTR_1g064770 42.188 64 36 1 337 399 28 91 3.13e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32581.t1 AT4G13460 58.171 514 205 6 6 511 130 641 0.0 613
MS.gene32581.t1 AT4G13460 58.171 514 205 6 6 511 130 641 0.0 613
MS.gene32581.t1 AT2G33290 55.273 512 222 4 6 512 134 643 0.0 583
MS.gene32581.t1 AT2G33290 55.273 512 222 4 6 512 134 643 0.0 583
MS.gene32581.t1 AT2G33290 55.273 512 222 4 6 512 134 643 0.0 583
MS.gene32581.t1 AT2G33290 55.273 512 222 4 6 512 134 643 0.0 583
MS.gene32581.t1 AT5G04940 36.673 499 294 13 21 509 155 641 4.46e-99 313
MS.gene32581.t1 AT5G04940 36.673 499 294 13 21 509 155 641 4.46e-99 313
MS.gene32581.t1 AT1G73100 38.105 475 270 12 56 519 189 650 3.57e-98 311
MS.gene32581.t1 AT2G22740 34.241 514 305 13 21 516 270 768 1.58e-83 275
MS.gene32581.t1 AT2G22740 34.241 514 305 13 21 516 270 768 1.58e-83 275
MS.gene32581.t1 AT1G17770 36.111 468 270 12 57 509 209 662 3.65e-82 270
MS.gene32581.t1 AT2G35160 35.498 462 254 12 56 511 309 732 1.13e-81 270
MS.gene32581.t1 AT2G35160 35.498 462 254 12 56 511 345 768 2.58e-81 270
MS.gene32581.t1 AT2G35160 35.498 462 254 12 56 511 345 768 2.58e-81 270
MS.gene32581.t1 AT2G35160 35.498 462 254 12 56 511 345 768 2.58e-81 270
MS.gene32581.t1 AT2G35160 35.498 462 254 12 56 511 345 768 2.58e-81 270
MS.gene32581.t1 AT5G13960 34.702 487 287 12 47 511 121 598 4.46e-81 265
MS.gene32581.t1 AT5G13960 34.728 478 276 11 55 511 2 464 8.15e-81 261
MS.gene32581.t1 AT2G24740 34.839 465 272 14 52 509 285 725 5.87e-67 230
MS.gene32581.t1 AT5G47150 37.387 222 129 5 10 229 111 324 3.86e-29 117
MS.gene32581.t1 AT5G47160 35.349 215 127 6 14 225 202 407 1.80e-25 108
MS.gene32581.t1 AT5G47160 35.349 215 127 6 14 225 202 407 1.80e-25 108
MS.gene32581.t1 AT5G47160 35.349 215 127 6 14 225 202 407 1.80e-25 108
MS.gene32581.t1 AT2G05900 36.471 170 104 3 117 286 26 191 2.69e-21 95.1
MS.gene32581.t1 AT2G23740 31.579 190 106 5 325 507 1184 1356 9.44e-19 90.9
MS.gene32581.t1 AT2G23740 31.579 190 106 5 325 507 1184 1356 9.44e-19 90.9
MS.gene32581.t1 AT2G23740 31.579 190 106 5 325 507 1184 1356 9.44e-19 90.9
MS.gene32581.t1 AT2G23740 31.579 190 106 5 325 507 1177 1349 1.06e-18 90.5
MS.gene32581.t1 AT1G57820 32.484 157 92 4 80 229 277 426 4.79e-15 78.6
MS.gene32581.t1 AT1G57820 32.484 157 92 4 80 229 277 426 4.79e-15 78.6
MS.gene32581.t1 AT3G03750 29.808 208 120 6 301 507 141 323 8.69e-15 76.3
MS.gene32581.t1 AT2G23740 46.914 81 40 2 325 403 1184 1263 4.27e-14 75.9
MS.gene32581.t1 AT2G23740 46.914 81 40 2 325 403 1184 1263 4.27e-14 75.9
MS.gene32581.t1 AT1G57820 31.818 154 94 4 80 229 277 423 5.91e-14 75.1

Find 141 sgRNAs with CRISPR-Local

Find 162 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTACTAGTGTGATTGTTTC+TGG 0.079078 1.3:-19560719 MS.gene32581:CDS
GTTGGAAGTGTTTAGATCTT+TGG 0.227926 1.3:-19559985 MS.gene32581:CDS
CTATGATTCGGTTCGTGTTT+TGG 0.242077 1.3:-19560990 MS.gene32581:CDS
AGAAGCCCGCTTTGACTATA+AGG 0.293128 1.3:+19560104 None:intergenic
AGAGATTTCAGCCTTGATTA+TGG 0.322787 1.3:-19559582 MS.gene32581:CDS
AGGATTGTTGGCGCGATTCC+CGG 0.341645 1.3:-19560863 MS.gene32581:CDS
CCCCATTCCGTCCACCTATT+CGG 0.341830 1.3:+19559822 None:intergenic
CCATTTGAACTCATACTTCC+AGG 0.343923 1.3:+19560752 None:intergenic
TCAAGCTAAGATGGGTAGTT+TGG 0.350493 1.3:-19560396 MS.gene32581:CDS
TTGTGTGTGGTTGGTTTACA+TGG 0.355893 1.3:-19560803 MS.gene32581:CDS
GGATGGAGCGTGTTGGTATA+AGG 0.360343 1.3:-19560295 MS.gene32581:CDS
GTGTTTAGATCTTTGGAAAC+TGG 0.365217 1.3:-19559978 MS.gene32581:CDS
ATGGAGAGGAGTATGCATTA+TGG 0.365705 1.3:-19560581 MS.gene32581:CDS
TAGATCTTTGGAAACTGGTT+GGG 0.370326 1.3:-19559973 MS.gene32581:CDS
TGCATTTGTTTCATGAGAAA+TGG 0.371968 1.3:-19560137 MS.gene32581:CDS
CTTGTTCGAATGACGGATCT+AGG 0.373790 1.3:-19561064 MS.gene32581:CDS
GTAAATGTGTGGGCAGTTGC+AGG 0.381411 1.3:-19560166 MS.gene32581:CDS
GATGTTGGTAAAAGTGGTTT+TGG 0.382231 1.3:-19560452 MS.gene32581:CDS
TCCATCCCGTTGGAAATATC+AGG 0.385210 1.3:+19560311 None:intergenic
GAAGGTACTTCTTCAACTTC+AGG 0.391726 1.3:-19560524 MS.gene32581:CDS
TCCGAATAGGTGGACGGAAT+GGG 0.393302 1.3:-19559823 MS.gene32581:CDS
CATTTATATGCGAGTACAGC+GGG 0.395589 1.3:-19559917 MS.gene32581:CDS
TAAATGTGCCAGCTTGAATA+AGG 0.408878 1.3:+19559934 None:intergenic
GCGATTCCCGGGATTTGTAT+TGG 0.410719 1.3:-19560851 MS.gene32581:CDS
TGGAGAGGAGTATGCATTAT+GGG 0.415278 1.3:-19560580 MS.gene32581:CDS
TTCTGCTACGATGAGGAGTC+GGG 0.432959 1.3:-19560909 MS.gene32581:CDS
GGATTGTTGGCGCGATTCCC+GGG 0.437413 1.3:-19560862 MS.gene32581:CDS
GCTGGGATCGATTATTTGCC+TGG 0.440591 1.3:-19560770 MS.gene32581:CDS
GATTGTTTCTGGTGGATATG+AGG 0.443033 1.3:-19560708 MS.gene32581:CDS
GGTAAGGTTTATGTTTATGA+TGG 0.443436 1.3:-19560503 MS.gene32581:CDS
CACAACAAGGGCTTAATCAT+AGG 0.446724 1.3:-19560007 MS.gene32581:CDS
GTAGCTGTCCGCCTCATTGT+CGG 0.447353 1.3:-19560040 MS.gene32581:CDS
TCCCTTTGAGTTTCATCCAA+GGG 0.453527 1.3:-19560204 MS.gene32581:CDS
AAATTCAAAGGAAGAATTGA+TGG 0.457852 1.3:+19559756 None:intergenic
GACTAGGATGGTCTATGATT+CGG 0.457902 1.3:-19561002 MS.gene32581:CDS
TGTATAAGATTGTTGAAAGC+TGG 0.458955 1.3:-19560478 MS.gene32581:CDS
CCTGGAAGTATGAGTTCAAA+TGG 0.460753 1.3:-19560752 MS.gene32581:CDS
GATTGATGATCAAGCTAAGA+TGG 0.462647 1.3:-19560405 MS.gene32581:CDS
AATCGCAATATGAGGATTGT+TGG 0.469750 1.3:-19560875 MS.gene32581:CDS
CGATGAGGAGTCGGGGATTG+TGG 0.470344 1.3:-19560901 MS.gene32581:CDS
ACAAACTGAACCATCAGATT+AGG 0.470806 1.3:+19559675 None:intergenic
ATACACATAGGTTTATAATC+AGG 0.471074 1.3:+19560341 None:intergenic
CACATCACCAATACAAATCC+CGG 0.471185 1.3:+19560844 None:intergenic
TGCATTATGGGATTGAGGTA+AGG 0.472341 1.3:-19560568 MS.gene32581:CDS
TTCCGAATAGGTGGACGGAA+TGG 0.473704 1.3:-19559824 MS.gene32581:CDS
TACTTCTTCAACTTCAGGTA+AGG 0.478043 1.3:-19560519 MS.gene32581:CDS
AGGTGATGTTATTATTCTCT+CGG 0.481641 1.3:-19560672 MS.gene32581:CDS
GTTATTATTCTCTCGGGTCA+TGG 0.481758 1.3:-19560665 MS.gene32581:CDS
AGGATGGAGTTGTGTGTGGT+TGG 0.488005 1.3:-19560812 MS.gene32581:CDS
ATCGGAAAAGCGTCAAATGC+GGG 0.499046 1.3:-19561038 MS.gene32581:CDS
AATGACGGATCTAGGGTTAT+CGG 0.500590 1.3:-19561056 MS.gene32581:CDS
TGTGGCTGAATCGCAATATG+AGG 0.502406 1.3:-19560883 MS.gene32581:CDS
AGATCTTTGGAAACTGGTTG+GGG 0.504345 1.3:-19559972 MS.gene32581:CDS
GGTGATGTTATTATTCTCTC+GGG 0.504399 1.3:-19560671 MS.gene32581:CDS
ATTGTTTCTGGTGGATATGA+GGG 0.508794 1.3:-19560707 MS.gene32581:CDS
TGGTGTGATGTTGGTAAAAG+TGG 0.509931 1.3:-19560458 MS.gene32581:CDS
TTCCCTTTGAGTTTCATCCA+AGG 0.510250 1.3:-19560205 MS.gene32581:CDS
CTTCTGCTACGATGAGGAGT+CGG 0.510457 1.3:-19560910 MS.gene32581:CDS
TATCTCCTGATATTTCCAAC+GGG 0.517150 1.3:-19560316 MS.gene32581:CDS
TCATTTGACCTTATTCAAGC+TGG 0.519192 1.3:-19559942 MS.gene32581:CDS
TAAAACTATGCTGTTTGCGA+TGG 0.519235 1.3:-19559622 MS.gene32581:CDS
CCCCTTGGATGAAACTCAAA+GGG 0.519814 1.3:+19560203 None:intergenic
CGGAACCGTGTTACACAACA+AGG 0.524134 1.3:-19560020 MS.gene32581:CDS
ACAATCACACTAGTAGCAAT+CGG 0.525852 1.3:+19560725 None:intergenic
TCTCTCGGGTCATGGTGGGC+AGG 0.526040 1.3:-19560657 MS.gene32581:CDS
TGGAAACTGGTTGGGGAGTG+AGG 0.530566 1.3:-19559965 MS.gene32581:CDS
TGAGGCGGACAGCTACAATC+AGG 0.531632 1.3:+19560047 None:intergenic
GAGTATGCATTATGGGATTG+AGG 0.534486 1.3:-19560573 MS.gene32581:CDS
GCTTTCCTTATAGTCAAAGC+GGG 0.536291 1.3:-19560109 MS.gene32581:CDS
GTTGAAAGCTGGTGTGATGT+TGG 0.536898 1.3:-19560467 MS.gene32581:CDS
ACTAGTGTGATTGTTTCTGG+TGG 0.538664 1.3:-19560716 MS.gene32581:CDS
TCATCAGAAGCTAGAAGGAG+GGG 0.538962 1.3:-19560612 MS.gene32581:CDS
TTAGATCTTTGGAAACTGGT+TGG 0.541400 1.3:-19559974 MS.gene32581:CDS
TTGGATATGGCTGAAGATGA+AGG 0.542424 1.3:-19560971 MS.gene32581:CDS
TTGTTCGAATGACGGATCTA+GGG 0.543701 1.3:-19561063 MS.gene32581:CDS
TCCTGATATTTCCAACGGGA+TGG 0.545378 1.3:-19560312 MS.gene32581:CDS
GCATTATGGGATTGAGGTAA+GGG 0.545569 1.3:-19560567 MS.gene32581:CDS
TTATTCTCTCGGGTCATGGT+GGG 0.547783 1.3:-19560661 MS.gene32581:CDS
TCAAGGCTGAAATCTCTCAA+TGG 0.553800 1.3:+19559588 None:intergenic
CAAAGCGGGCTTCTCTTGAA+AGG 0.554951 1.3:-19560095 MS.gene32581:CDS
ACAAGGGCTTAATCATAGGT+TGG 0.555207 1.3:-19560003 MS.gene32581:CDS
AGAAGGAGGGGATCTTGCTA+TGG 0.555793 1.3:-19560600 MS.gene32581:CDS
ATATCTCCTGATATTTCCAA+CGG 0.556018 1.3:-19560317 MS.gene32581:CDS
GCTTCTCTTGAAAGGAAAGC+CGG 0.556611 1.3:-19560087 MS.gene32581:CDS
AGATCCGTCATTCGAACAAG+TGG 0.563268 1.3:+19561067 None:intergenic
CCGAATAGGTGGACGGAATG+GGG 0.564405 1.3:-19559822 MS.gene32581:CDS
GAAATCTCTCAATGGAGGGA+TGG 0.567762 1.3:+19559596 None:intergenic
TTACATGGTGAACCACAAGC+TGG 0.570814 1.3:-19560788 MS.gene32581:CDS
CTCTGGACGTGTCTAGAATG+AGG 0.571022 1.3:-19559731 MS.gene32581:CDS
CCCTTTGAGTTTCATCCAAG+GGG 0.573842 1.3:-19560203 MS.gene32581:CDS
TTCATCAGAAGCTAGAAGGA+GGG 0.573844 1.3:-19560613 MS.gene32581:CDS
GTATAGGATGGAGTTGTGTG+TGG 0.574388 1.3:-19560816 MS.gene32581:CDS
GGCTGAAATCTCTCAATGGA+GGG 0.575518 1.3:+19559592 None:intergenic
TACATGGTGAACCACAAGCT+GGG 0.577042 1.3:-19560787 MS.gene32581:CDS
GGGATGTTGTTCGAAGGACT+AGG 0.579467 1.3:-19561018 MS.gene32581:CDS
TAGCTCAACTCCTAATCTGA+TGG 0.582333 1.3:-19559685 MS.gene32581:CDS
ATATTCAATTGCGCATTGCG+AGG 0.586032 1.3:+19559532 None:intergenic
CATCAGCTACACCATAATCA+AGG 0.586256 1.3:+19559571 None:intergenic
ATGGTGGGCAGGATAAGAAT+CGG 0.592281 1.3:-19560646 MS.gene32581:CDS
ATTATTCTCTCGGGTCATGG+TGG 0.592834 1.3:-19560662 MS.gene32581:CDS
ATTGATGATCAAGCTAAGAT+GGG 0.593619 1.3:-19560404 MS.gene32581:CDS
GGAAGAATTGATGGATACGA+AGG 0.593629 1.3:+19559765 None:intergenic
TAAGCCCTTGTTGTGTAACA+CGG 0.598036 1.3:+19560015 None:intergenic
AGGCTGAAATCTCTCAATGG+AGG 0.598790 1.3:+19559591 None:intergenic
GATCCCACTTGTTCGAATGA+CGG 0.598813 1.3:-19561071 MS.gene32581:CDS
ACCAACACGCTCCATCCCGT+TGG 0.599403 1.3:+19560301 None:intergenic
ACAGCGGGGTTGTTCTAACA+AGG 0.600545 1.3:-19559902 MS.gene32581:CDS
TTTCATCAGAAGCTAGAAGG+AGG 0.601171 1.3:-19560614 MS.gene32581:CDS
GCAGAAATTATGACTATGAG+TGG 0.602784 1.3:-19559873 MS.gene32581:CDS
ACGTCCAGAGCAAAATTCAA+AGG 0.609054 1.3:+19559744 None:intergenic
ACATCACCAATACAAATCCC+GGG 0.611261 1.3:+19560845 None:intergenic
TGTGTGGGCAGTTGCAGGGA+AGG 0.614573 1.3:-19560161 MS.gene32581:CDS
GAGGGGATCTTGCTATGGAG+AGG 0.622960 1.3:-19560595 MS.gene32581:CDS
TCAGGAGATATAATACACAT+AGG 0.624086 1.3:+19560329 None:intergenic
TGAGACAGGTTGTAAATGTG+TGG 0.625021 1.3:-19560177 MS.gene32581:CDS
TATCGGAAAAGCGTCAAATG+CGG 0.625063 1.3:-19561039 MS.gene32581:CDS
ATCCGCGCTGTGAGGTACGA+AGG 0.625946 1.3:-19560542 MS.gene32581:CDS
GGAAGATACTCAAAATACAT+CGG 0.630650 1.3:+19560242 None:intergenic
AGAATTGATGGATACGAAGG+AGG 0.631640 1.3:+19559768 None:intergenic
AAATAATCGATCCCAGCTTG+TGG 0.632977 1.3:+19560776 None:intergenic
TATGAGGGTGATGTTGATGA+AGG 0.637266 1.3:-19560692 MS.gene32581:CDS
AGCTTTCCTTATAGTCAAAG+CGG 0.639771 1.3:-19560110 MS.gene32581:CDS
TAAATGTGTGGGCAGTTGCA+GGG 0.639917 1.3:-19560165 MS.gene32581:CDS
AAATGCGGGATGTTGTTCGA+AGG 0.647284 1.3:-19561024 MS.gene32581:CDS
GAGACAGGTTGTAAATGTGT+GGG 0.652888 1.3:-19560176 MS.gene32581:CDS
TGTTGTTCGAAGGACTAGGA+TGG 0.654135 1.3:-19561014 MS.gene32581:CDS
TGAAAGCTTCTGCTACGATG+AGG 0.658793 1.3:-19560916 MS.gene32581:CDS
CCTTTGAGTTTCATCCAAGG+GGG 0.661963 1.3:-19560202 MS.gene32581:CDS
TCCAACGGGATGGAGCGTGT+TGG 0.665494 1.3:-19560302 MS.gene32581:CDS
TAAGGGTCATCCGCGCTGTG+AGG 0.665597 1.3:-19560550 MS.gene32581:CDS
GATCCGTCATTCGAACAAGT+GGG 0.669479 1.3:+19561068 None:intergenic
ACATTTATATGCGAGTACAG+CGG 0.676169 1.3:-19559918 MS.gene32581:CDS
ACACGGTTCCGACAATGAGG+CGG 0.681770 1.3:+19560032 None:intergenic
TCTGCTACGATGAGGAGTCG+GGG 0.683469 1.3:-19560908 MS.gene32581:CDS
GTGGGCAGGATAAGAATCGG+AGG 0.693208 1.3:-19560643 MS.gene32581:CDS
CAGCGGGGTTGTTCTAACAA+GGG 0.700564 1.3:-19559901 MS.gene32581:CDS
GTAACACGGTTCCGACAATG+AGG 0.703986 1.3:+19560029 None:intergenic
CGTGTCTAGAATGAGGAATG+TGG 0.710491 1.3:-19559724 MS.gene32581:CDS
ATTTATATGCGAGTACAGCG+GGG 0.724918 1.3:-19559916 MS.gene32581:CDS
TACCTTCGTACCTCACAGCG+CGG 0.749117 1.3:+19560540 None:intergenic
GGAACCGTGTTACACAACAA+GGG 0.763258 1.3:-19560019 MS.gene32581:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAATTCAAAGGAAGAATTGA+TGG + chr1.3:19560862-19560881 None:intergenic 25.0%
! ATACACATAGGTTTATAATC+AGG + chr1.3:19560277-19560296 None:intergenic 25.0%
!!! TTTTTAAGTTTATGTTGTCG+AGG - chr1.3:19560188-19560207 MS.gene32581:CDS 25.0%
AGGTGATGTTATTATTCTCT+CGG - chr1.3:19559943-19559962 MS.gene32581:CDS 30.0%
ATATCTCCTGATATTTCCAA+CGG - chr1.3:19560298-19560317 MS.gene32581:CDS 30.0%
GGAAGATACTCAAAATACAT+CGG + chr1.3:19560376-19560395 None:intergenic 30.0%
GGTAAGGTTTATGTTTATGA+TGG - chr1.3:19560112-19560131 MS.gene32581:CDS 30.0%
TCAGGAGATATAATACACAT+AGG + chr1.3:19560289-19560308 None:intergenic 30.0%
TGCATTTGTTTCATGAGAAA+TGG - chr1.3:19560478-19560497 MS.gene32581:CDS 30.0%
TGTATAAGATTGTTGAAAGC+TGG - chr1.3:19560137-19560156 MS.gene32581:CDS 30.0%
! ATTGATGATCAAGCTAAGAT+GGG - chr1.3:19560211-19560230 MS.gene32581:CDS 30.0%
!! GTATTTTGAGTATCTTCCTA+GGG - chr1.3:19560378-19560397 MS.gene32581:CDS 30.0%
!! TGATGTGTTTTTGTATAGGA+TGG - chr1.3:19559787-19559806 MS.gene32581:CDS 30.0%
!! TGTATTTTGAGTATCTTCCT+AGG - chr1.3:19560377-19560396 MS.gene32581:CDS 30.0%
!!! TTGGTGATGTGTTTTTGTAT+AGG - chr1.3:19559783-19559802 MS.gene32581:CDS 30.0%
AATCGCAATATGAGGATTGT+TGG - chr1.3:19559740-19559759 MS.gene32581:CDS 35.0%
ACAAACTGAACCATCAGATT+AGG + chr1.3:19560943-19560962 None:intergenic 35.0%
ACAATCACACTAGTAGCAAT+CGG + chr1.3:19559893-19559912 None:intergenic 35.0%
ACATTTATATGCGAGTACAG+CGG - chr1.3:19560697-19560716 MS.gene32581:CDS 35.0%
AGAGATTTCAGCCTTGATTA+TGG - chr1.3:19561033-19561052 MS.gene32581:CDS 35.0%
AGCTTTCCTTATAGTCAAAG+CGG - chr1.3:19560505-19560524 MS.gene32581:CDS 35.0%
CACCTATTCGGAAAAAGATT+CGG + chr1.3:19560784-19560803 None:intergenic 35.0%
GCAGAAATTATGACTATGAG+TGG - chr1.3:19560742-19560761 MS.gene32581:CDS 35.0%
GGTGATGTTATTATTCTCTC+GGG - chr1.3:19559944-19559963 MS.gene32581:CDS 35.0%
TAAATGTGCCAGCTTGAATA+AGG + chr1.3:19560684-19560703 None:intergenic 35.0%
TACTTCTTCAACTTCAGGTA+AGG - chr1.3:19560096-19560115 MS.gene32581:CDS 35.0%
TATCTCCTGATATTTCCAAC+GGG - chr1.3:19560299-19560318 MS.gene32581:CDS 35.0%
TCATTTGACCTTATTCAAGC+TGG - chr1.3:19560673-19560692 MS.gene32581:CDS 35.0%
! ATCCGAATCTTTTTCCGAAT+AGG - chr1.3:19560779-19560798 MS.gene32581:CDS 35.0%
! GATTGATGATCAAGCTAAGA+TGG - chr1.3:19560210-19560229 MS.gene32581:CDS 35.0%
! GTGTTTAGATCTTTGGAAAC+TGG - chr1.3:19560637-19560656 MS.gene32581:CDS 35.0%
! GTTTTTCATCAGAAGCTAGA+AGG - chr1.3:19559998-19560017 MS.gene32581:CDS 35.0%
! TAAAACTATGCTGTTTGCGA+TGG - chr1.3:19560993-19561012 MS.gene32581:CDS 35.0%
! TAGATCTTTGGAAACTGGTT+GGG - chr1.3:19560642-19560661 MS.gene32581:CDS 35.0%
! TCTTCCTTTGAATTTTGCTC+TGG - chr1.3:19560867-19560886 MS.gene32581:CDS 35.0%
! TTAGATCTTTGGAAACTGGT+TGG - chr1.3:19560641-19560660 MS.gene32581:CDS 35.0%
!! ATTGTTTCTGGTGGATATGA+GGG - chr1.3:19559908-19559927 MS.gene32581:CDS 35.0%
!! GATGTTGGTAAAAGTGGTTT+TGG - chr1.3:19560163-19560182 MS.gene32581:CDS 35.0%
!! GTTGGAAGTGTTTAGATCTT+TGG - chr1.3:19560630-19560649 MS.gene32581:CDS 35.0%
!! TCGCAAACAGCATAGTTTTA+AGG + chr1.3:19560993-19561012 None:intergenic 35.0%
!!! GATGGGTAGTTTGGTTTTAA+AGG - chr1.3:19560228-19560247 MS.gene32581:CDS 35.0%
AAATAATCGATCCCAGCTTG+TGG + chr1.3:19559842-19559861 None:intergenic 40.0%
AAGGGAAAAAAAGTAGCCCT+AGG + chr1.3:19560397-19560416 None:intergenic 40.0%
ACAAGGGCTTAATCATAGGT+TGG - chr1.3:19560612-19560631 MS.gene32581:CDS 40.0%
ACATCACCAATACAAATCCC+GGG + chr1.3:19559773-19559792 None:intergenic 40.0%
ACGTCCAGAGCAAAATTCAA+AGG + chr1.3:19560874-19560893 None:intergenic 40.0%
ATTTATATGCGAGTACAGCG+GGG - chr1.3:19560699-19560718 MS.gene32581:CDS 40.0%
CACAACAAGGGCTTAATCAT+AGG - chr1.3:19560608-19560627 MS.gene32581:CDS 40.0%
CACATCACCAATACAAATCC+CGG + chr1.3:19559774-19559793 None:intergenic 40.0%
CATCAGCTACACCATAATCA+AGG + chr1.3:19561047-19561066 None:intergenic 40.0%
CATTTATATGCGAGTACAGC+GGG - chr1.3:19560698-19560717 MS.gene32581:CDS 40.0%
CCATTTGAACTCATACTTCC+AGG + chr1.3:19559866-19559885 None:intergenic 40.0%
GACTAGGATGGTCTATGATT+CGG - chr1.3:19559613-19559632 MS.gene32581:CDS 40.0%
GAGACAGGTTGTAAATGTGT+GGG - chr1.3:19560439-19560458 MS.gene32581:CDS 40.0%
GAGTATGCATTATGGGATTG+AGG - chr1.3:19560042-19560061 MS.gene32581:CDS 40.0%
GCATTATGGGATTGAGGTAA+GGG - chr1.3:19560048-19560067 MS.gene32581:CDS 40.0%
GCTACTAGTGTGATTGTTTC+TGG - chr1.3:19559896-19559915 MS.gene32581:CDS 40.0%
GCTTTCCTTATAGTCAAAGC+GGG - chr1.3:19560506-19560525 MS.gene32581:CDS 40.0%
GGACCACATTCAAAAATGAC+CGG + chr1.3:19560550-19560569 None:intergenic 40.0%
GTTATTATTCTCTCGGGTCA+TGG - chr1.3:19559950-19559969 MS.gene32581:CDS 40.0%
TAGCTCAACTCCTAATCTGA+TGG - chr1.3:19560930-19560949 MS.gene32581:CDS 40.0%
TCAAGCTAAGATGGGTAGTT+TGG - chr1.3:19560219-19560238 MS.gene32581:CDS 40.0%
TCAAGGCTGAAATCTCTCAA+TGG + chr1.3:19561030-19561049 None:intergenic 40.0%
TGAGACAGGTTGTAAATGTG+TGG - chr1.3:19560438-19560457 MS.gene32581:CDS 40.0%
TGCATTATGGGATTGAGGTA+AGG - chr1.3:19560047-19560066 MS.gene32581:CDS 40.0%
TTCATCAGAAGCTAGAAGGA+GGG - chr1.3:19560002-19560021 MS.gene32581:CDS 40.0%
TTGGATATGGCTGAAGATGA+AGG - chr1.3:19559644-19559663 MS.gene32581:CDS 40.0%
TTTCATCAGAAGCTAGAAGG+AGG - chr1.3:19560001-19560020 MS.gene32581:CDS 40.0%
! AGAATTGATGGATACGAAGG+AGG + chr1.3:19560850-19560869 None:intergenic 40.0%
! AGATCTTTGGAAACTGGTTG+GGG - chr1.3:19560643-19560662 MS.gene32581:CDS 40.0%
! ATGGAGAGGAGTATGCATTA+TGG - chr1.3:19560034-19560053 MS.gene32581:CDS 40.0%
! CCTGGAAGTATGAGTTCAAA+TGG - chr1.3:19559863-19559882 MS.gene32581:CDS 40.0%
! CGAATCTTTTTCCGAATAGG+TGG - chr1.3:19560782-19560801 MS.gene32581:CDS 40.0%
! GGAAGAATTGATGGATACGA+AGG + chr1.3:19560853-19560872 None:intergenic 40.0%
! TAAGCCCTTGTTGTGTAACA+CGG + chr1.3:19560603-19560622 None:intergenic 40.0%
! TATGAGGGTGATGTTGATGA+AGG - chr1.3:19559923-19559942 MS.gene32581:CDS 40.0%
! TCCCTTTGAGTTTCATCCAA+GGG - chr1.3:19560411-19560430 MS.gene32581:CDS 40.0%
! TCTTTTTCCGAATAGGTGGA+CGG - chr1.3:19560786-19560805 MS.gene32581:CDS 40.0%
! TGGAGAGGAGTATGCATTAT+GGG - chr1.3:19560035-19560054 MS.gene32581:CDS 40.0%
! TGGTGTGATGTTGGTAAAAG+TGG - chr1.3:19560157-19560176 MS.gene32581:CDS 40.0%
! TTCCCTTTGAGTTTCATCCA+AGG - chr1.3:19560410-19560429 MS.gene32581:CDS 40.0%
!! AATGACGGATCTAGGGTTAT+CGG - chr1.3:19559559-19559578 MS.gene32581:CDS 40.0%
!! ACTAGTGTGATTGTTTCTGG+TGG - chr1.3:19559899-19559918 MS.gene32581:CDS 40.0%
!! GAAGGTACTTCTTCAACTTC+AGG - chr1.3:19560091-19560110 MS.gene32581:CDS 40.0%
!! GATTGTTTCTGGTGGATATG+AGG - chr1.3:19559907-19559926 MS.gene32581:CDS 40.0%
!! TATCGGAAAAGCGTCAAATG+CGG - chr1.3:19559576-19559595 MS.gene32581:CDS 40.0%
!! TTCGGTTCGTGTTTTGGATA+TGG - chr1.3:19559631-19559650 MS.gene32581:CDS 40.0%
!! TTGTGTGTGGTTGGTTTACA+TGG - chr1.3:19559812-19559831 MS.gene32581:CDS 40.0%
!! TTGTTCGAATGACGGATCTA+GGG - chr1.3:19559552-19559571 MS.gene32581:CDS 40.0%
!!! AAGCCGGTCATTTTTGAATG+TGG - chr1.3:19560544-19560563 MS.gene32581:CDS 40.0%
!!! CTATGATTCGGTTCGTGTTT+TGG - chr1.3:19559625-19559644 MS.gene32581:CDS 40.0%
!!! GGGTAGTTTGGTTTTAAAGG+AGG - chr1.3:19560231-19560250 MS.gene32581:CDS 40.0%
!!! TAAAGGAGGCTTTTTTGCGT+AGG - chr1.3:19560245-19560264 MS.gene32581:CDS 40.0%
AAATGCGGGATGTTGTTCGA+AGG - chr1.3:19559591-19559610 MS.gene32581:CDS 45.0%
AGAAGCCCGCTTTGACTATA+AGG + chr1.3:19560514-19560533 None:intergenic 45.0%
AGATCCGTCATTCGAACAAG+TGG + chr1.3:19559551-19559570 None:intergenic 45.0%
AGGCTGAAATCTCTCAATGG+AGG + chr1.3:19561027-19561046 None:intergenic 45.0%
ATTATTCTCTCGGGTCATGG+TGG - chr1.3:19559953-19559972 MS.gene32581:CDS 45.0%
CCCCTTGGATGAAACTCAAA+GGG + chr1.3:19560415-19560434 None:intergenic 45.0%
GAAATCTCTCAATGGAGGGA+TGG + chr1.3:19561022-19561041 None:intergenic 45.0%
GATCCCACTTGTTCGAATGA+CGG - chr1.3:19559544-19559563 MS.gene32581:CDS 45.0%
GATCCGTCATTCGAACAAGT+GGG + chr1.3:19559550-19559569 None:intergenic 45.0%
GCTTCTCTTGAAAGGAAAGC+CGG - chr1.3:19560528-19560547 MS.gene32581:CDS 45.0%
GGAACCGTGTTACACAACAA+GGG - chr1.3:19560596-19560615 MS.gene32581:CDS 45.0%
GGCTGAAATCTCTCAATGGA+GGG + chr1.3:19561026-19561045 None:intergenic 45.0%
GTATAGGATGGAGTTGTGTG+TGG - chr1.3:19559799-19559818 MS.gene32581:CDS 45.0%
GTTGAAAGCTGGTGTGATGT+TGG - chr1.3:19560148-19560167 MS.gene32581:CDS 45.0%
TAAATGTGTGGGCAGTTGCA+GGG - chr1.3:19560450-19560469 MS.gene32581:CDS 45.0%
TACATGGTGAACCACAAGCT+GGG - chr1.3:19559828-19559847 MS.gene32581:CDS 45.0%
TCATCAGAAGCTAGAAGGAG+GGG - chr1.3:19560003-19560022 MS.gene32581:CDS 45.0%
TCCATCCCGTTGGAAATATC+AGG + chr1.3:19560307-19560326 None:intergenic 45.0%
TCCTGATATTTCCAACGGGA+TGG - chr1.3:19560303-19560322 MS.gene32581:CDS 45.0%
TGAAAGCTTCTGCTACGATG+AGG - chr1.3:19559699-19559718 MS.gene32581:CDS 45.0%
TGTGGCTGAATCGCAATATG+AGG - chr1.3:19559732-19559751 MS.gene32581:CDS 45.0%
TGTTGTTCGAAGGACTAGGA+TGG - chr1.3:19559601-19559620 MS.gene32581:CDS 45.0%
TTACATGGTGAACCACAAGC+TGG - chr1.3:19559827-19559846 MS.gene32581:CDS 45.0%
TTATTCTCTCGGGTCATGGT+GGG - chr1.3:19559954-19559973 MS.gene32581:CDS 45.0%
! CCCTTTGAGTTTCATCCAAG+GGG - chr1.3:19560412-19560431 MS.gene32581:CDS 45.0%
! CCTTTGAGTTTCATCCAAGG+GGG - chr1.3:19560413-19560432 MS.gene32581:CDS 45.0%
! CGTGTCTAGAATGAGGAATG+TGG - chr1.3:19560891-19560910 MS.gene32581:CDS 45.0%
!! ATCGGAAAAGCGTCAAATGC+GGG - chr1.3:19559577-19559596 MS.gene32581:CDS 45.0%
!! ATGGTGGGCAGGATAAGAAT+CGG - chr1.3:19559969-19559988 MS.gene32581:CDS 45.0%
!! CTTGTTCGAATGACGGATCT+AGG - chr1.3:19559551-19559570 MS.gene32581:CDS 45.0%
ACAGCGGGGTTGTTCTAACA+AGG - chr1.3:19560713-19560732 MS.gene32581:CDS 50.0%
AGAAGGAGGGGATCTTGCTA+TGG - chr1.3:19560015-19560034 MS.gene32581:CDS 50.0%
AGGATGGAGTTGTGTGTGGT+TGG - chr1.3:19559803-19559822 MS.gene32581:CDS 50.0%
CAGCGGGGTTGTTCTAACAA+GGG - chr1.3:19560714-19560733 MS.gene32581:CDS 50.0%
CCCCCTTGGATGAAACTCAA+AGG + chr1.3:19560416-19560435 None:intergenic 50.0%
CGGAACCGTGTTACACAACA+AGG - chr1.3:19560595-19560614 MS.gene32581:CDS 50.0%
CTTCTGCTACGATGAGGAGT+CGG - chr1.3:19559705-19559724 MS.gene32581:CDS 50.0%
GCGATTCCCGGGATTTGTAT+TGG - chr1.3:19559764-19559783 MS.gene32581:CDS 50.0%
GCTGGGATCGATTATTTGCC+TGG - chr1.3:19559845-19559864 MS.gene32581:CDS 50.0%
GGGATGTTGTTCGAAGGACT+AGG - chr1.3:19559597-19559616 MS.gene32581:CDS 50.0%
GTAAATGTGTGGGCAGTTGC+AGG - chr1.3:19560449-19560468 MS.gene32581:CDS 50.0%
GTAACACGGTTCCGACAATG+AGG + chr1.3:19560589-19560608 None:intergenic 50.0%
TCCGAATAGGTGGACGGAAT+GGG - chr1.3:19560792-19560811 MS.gene32581:CDS 50.0%
TTCCGAATAGGTGGACGGAA+TGG - chr1.3:19560791-19560810 MS.gene32581:CDS 50.0%
TTCTGCTACGATGAGGAGTC+GGG - chr1.3:19559706-19559725 MS.gene32581:CDS 50.0%
! CAAAGCGGGCTTCTCTTGAA+AGG - chr1.3:19560520-19560539 MS.gene32581:CDS 50.0%
! CTCTGGACGTGTCTAGAATG+AGG - chr1.3:19560884-19560903 MS.gene32581:CDS 50.0%
! GGATGGAGCGTGTTGGTATA+AGG - chr1.3:19560320-19560339 MS.gene32581:CDS 50.0%
ACACGGTTCCGACAATGAGG+CGG + chr1.3:19560586-19560605 None:intergenic 55.0%
AGGATTGTTGGCGCGATTCC+CGG - chr1.3:19559752-19559771 MS.gene32581:CDS 55.0%
CAACCTGTCTCATTCCCCCT+TGG + chr1.3:19560430-19560449 None:intergenic 55.0%
CATCCAAGGGGGAATGAGAC+AGG - chr1.3:19560424-19560443 MS.gene32581:CDS 55.0%
CCCCATTCCGTCCACCTATT+CGG + chr1.3:19560796-19560815 None:intergenic 55.0%
CCGAATAGGTGGACGGAATG+GGG - chr1.3:19560793-19560812 MS.gene32581:CDS 55.0%
GTAGCTGTCCGCCTCATTGT+CGG - chr1.3:19560575-19560594 MS.gene32581:CDS 55.0%
GTGGGCAGGATAAGAATCGG+AGG - chr1.3:19559972-19559991 MS.gene32581:CDS 55.0%
TACCTTCGTACCTCACAGCG+CGG + chr1.3:19560078-19560097 None:intergenic 55.0%
TCTGCTACGATGAGGAGTCG+GGG - chr1.3:19559707-19559726 MS.gene32581:CDS 55.0%
TGAGGCGGACAGCTACAATC+AGG + chr1.3:19560571-19560590 None:intergenic 55.0%
TGGAAACTGGTTGGGGAGTG+AGG - chr1.3:19560650-19560669 MS.gene32581:CDS 55.0%
! GAGGGGATCTTGCTATGGAG+AGG - chr1.3:19560020-19560039 MS.gene32581:CDS 55.0%
ACCAACACGCTCCATCCCGT+TGG + chr1.3:19560317-19560336 None:intergenic 60.0%
ATCCGCGCTGTGAGGTACGA+AGG - chr1.3:19560073-19560092 MS.gene32581:CDS 60.0%
CGATGAGGAGTCGGGGATTG+TGG - chr1.3:19559714-19559733 MS.gene32581:CDS 60.0%
GGATTGTTGGCGCGATTCCC+GGG - chr1.3:19559753-19559772 MS.gene32581:CDS 60.0%
TAAGGGTCATCCGCGCTGTG+AGG - chr1.3:19560065-19560084 MS.gene32581:CDS 60.0%
TCCAACGGGATGGAGCGTGT+TGG - chr1.3:19560313-19560332 MS.gene32581:CDS 60.0%
TGTGTGGGCAGTTGCAGGGA+AGG - chr1.3:19560454-19560473 MS.gene32581:CDS 60.0%
!! TCTCTCGGGTCATGGTGGGC+AGG - chr1.3:19559958-19559977 MS.gene32581:CDS 65.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 19559536 19561101 19559536 ID=MS.gene32581
chr1.3 mRNA 19559536 19561101 19559536 ID=MS.gene32581.t1;Parent=MS.gene32581
chr1.3 exon 19559536 19561101 19559536 ID=MS.gene32581.t1.exon1;Parent=MS.gene32581.t1
chr1.3 CDS 19559536 19561101 19559536 ID=cds.MS.gene32581.t1;Parent=MS.gene32581.t1
Gene Sequence

>MS.gene32581

ATGGCGATGATCCCACTTGTTCGAATGACGGATCTAGGGTTATCGGAAAAGCGTCAAATGCGGGATGTTGTTCGAAGGACTAGGATGGTCTATGATTCGGTTCGTGTTTTGGATATGGCTGAAGATGAAGGATATTTTAATGCTAGAAGAGTGAGAAGTGATTTGAAAGCTTCTGCTACGATGAGGAGTCGGGGATTGTGGCTGAATCGCAATATGAGGATTGTTGGCGCGATTCCCGGGATTTGTATTGGTGATGTGTTTTTGTATAGGATGGAGTTGTGTGTGGTTGGTTTACATGGTGAACCACAAGCTGGGATCGATTATTTGCCTGGAAGTATGAGTTCAAATGGCGATCCGATTGCTACTAGTGTGATTGTTTCTGGTGGATATGAGGGTGATGTTGATGAAGGTGATGTTATTATTCTCTCGGGTCATGGTGGGCAGGATAAGAATCGGAGGCAAGTTTTTCATCAGAAGCTAGAAGGAGGGGATCTTGCTATGGAGAGGAGTATGCATTATGGGATTGAGGTAAGGGTCATCCGCGCTGTGAGGTACGAAGGTACTTCTTCAACTTCAGGTAAGGTTTATGTTTATGATGGTTTGTATAAGATTGTTGAAAGCTGGTGTGATGTTGGTAAAAGTGGTTTTGGTGTTTTTAAGTTTATGTTGTCGAGGATTGATGATCAAGCTAAGATGGGTAGTTTGGTTTTAAAGGAGGCTTTTTTGCGTAGGAACGATCCTGATTATAAACCTATGTGTATTATATCTCCTGATATTTCCAACGGGATGGAGCGTGTTGGTATAAGGCTTTTTAATGATATTGATGAATGTAAAGATCCGATGTATTTTGAGTATCTTCCTAGGGCTACTTTTTTTCCCTTTGAGTTTCATCCAAGGGGGAATGAGACAGGTTGTAAATGTGTGGGCAGTTGCAGGGAAGGATGCATTTGTTTCATGAGAAATGGTAACAGCTTTCCTTATAGTCAAAGCGGGCTTCTCTTGAAAGGAAAGCCGGTCATTTTTGAATGTGGTCCTGATTGTAGCTGTCCGCCTCATTGTCGGAACCGTGTTACACAACAAGGGCTTAATCATAGGTTGGAAGTGTTTAGATCTTTGGAAACTGGTTGGGGAGTGAGGTCATTTGACCTTATTCAAGCTGGCACATTTATATGCGAGTACAGCGGGGTTGTTCTAACAAGGGAGCAAGCAGAAATTATGACTATGAGTGGTGATTCTTTAATATATCCGAATCTTTTTCCGAATAGGTGGACGGAATGGGGTAATTTATCTCTGATAGATGATGATTATGCGCCTCCTTCGTATCCATCAATTCTTCCTTTGAATTTTGCTCTGGACGTGTCTAGAATGAGGAATGTGGCTTGCTATATAAGTCATAGCTCAACTCCTAATCTGATGGTTCAGTTTGTTGTGTATGATCGCAATAATCAATTTTACCTTAAAACTATGCTGTTTGCGATGGAGACCATCCCTCCATTGAGAGATTTCAGCCTTGATTATGGTGTAGCTGATGATGTGTTGATTCGCGACCTCGCAATGCGCAATTGA
Protein sequence

>MS.gene32581.t1

MAMIPLVRMTDLGLSEKRQMRDVVRRTRMVYDSVRVLDMAEDEGYFNARRVRSDLKASATMRSRGLWLNRNMRIVGAIPGICIGDVFLYRMELCVVGLHGEPQAGIDYLPGSMSSNGDPIATSVIVSGGYEGDVDEGDVIILSGHGGQDKNRRQVFHQKLEGGDLAMERSMHYGIEVRVIRAVRYEGTSSTSGKVYVYDGLYKIVESWCDVGKSGFGVFKFMLSRIDDQAKMGSLVLKEAFLRRNDPDYKPMCIISPDISNGMERVGIRLFNDIDECKDPMYFEYLPRATFFPFEFHPRGNETGCKCVGSCREGCICFMRNGNSFPYSQSGLLLKGKPVIFECGPDCSCPPHCRNRVTQQGLNHRLEVFRSLETGWGVRSFDLIQAGTFICEYSGVVLTREQAEIMTMSGDSLIYPNLFPNRWTEWGNLSLIDDDYAPPSYPSILPLNFALDVSRMRNVACYISHSSTPNLMVQFVVYDRNNQFYLKTMLFAMETIPPLRDFSLDYGVADDVLIRDLAMRN