AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene32719

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32719.t1 MTR_3g105710 97.586 290 7 0 1 290 1 290 0.0 590
MS.gene32719.t1 MTR_1g013450 54.403 318 115 7 1 290 1 316 4.84e-106 311
MS.gene32719.t1 MTR_4g128930 51.205 332 101 11 4 280 20 345 5.91e-89 270
MS.gene32719.t1 MTR_7g018170 63.043 92 34 0 3 94 23 114 1.69e-37 137
MS.gene32719.t1 MTR_7g018170 71.429 70 9 2 231 289 326 395 6.44e-23 97.4
MS.gene32719.t1 MTR_5g021580 50.602 83 33 1 5 79 4 86 1.28e-20 87.4
MS.gene32719.t1 MTR_5g021580 50.602 83 33 1 5 79 4 86 1.38e-20 87.4
MS.gene32719.t1 MTR_5g069480 43.333 90 51 0 5 94 4 93 2.58e-19 86.3
MS.gene32719.t1 MTR_5g069480 43.333 90 51 0 5 94 4 93 3.25e-19 87.4
MS.gene32719.t1 MTR_1g023260 43.299 97 45 1 6 92 5 101 6.46e-19 84.7
MS.gene32719.t1 MTR_1g023260 43.299 97 45 1 6 92 5 101 6.64e-19 82.8
MS.gene32719.t1 MTR_2g073370 44.792 96 44 1 6 92 5 100 1.40e-18 84.0
MS.gene32719.t1 MTR_4g067320 43.750 96 45 1 6 92 5 100 1.42e-18 83.2
MS.gene32719.t1 MTR_4g067320 43.750 96 45 1 6 92 5 100 1.43e-18 83.2
MS.gene32719.t1 MTR_2g099010 42.708 96 46 1 6 92 5 100 1.91e-17 80.1
MS.gene32719.t1 MTR_2g099010 42.708 96 46 1 6 92 5 100 2.02e-17 80.1
MS.gene32719.t1 MTR_4g071200 39.175 97 49 1 6 92 5 101 7.32e-17 78.2
MS.gene32719.t1 MTR_3g117320 38.144 97 50 1 6 92 5 101 8.35e-17 78.2
MS.gene32719.t1 MTR_7g108150 39.326 89 53 1 6 94 20 107 3.90e-16 78.2
MS.gene32719.t1 MTR_3g082630 39.216 102 61 1 5 106 4 104 4.94e-16 78.2
MS.gene32719.t1 MTR_3g082630 44.872 78 40 1 202 279 327 401 4.11e-11 63.5
MS.gene32719.t1 MTR_3g034680 61.702 47 18 0 51 97 12 58 1.35e-15 71.2
MS.gene32719.t1 MTR_2g088900 45.122 82 45 0 5 86 100 181 8.58e-15 74.7
MS.gene32719.t1 MTR_2g088900 38.318 107 46 2 189 279 411 513 5.24e-12 66.2
MS.gene32719.t1 MTR_3g113070 37.895 95 48 1 6 89 5 99 1.15e-14 72.0
MS.gene32719.t1 MTR_7g083540 44.444 72 40 0 6 77 5 76 1.73e-13 70.5
MS.gene32719.t1 MTR_1g109350 39.189 74 45 0 4 77 3 76 1.98e-12 67.0
MS.gene32719.t1 MTR_1g110870 61.818 55 17 1 224 274 370 424 3.97e-12 66.6
MS.gene32719.t1 MTR_8g104190 70.455 44 13 0 232 275 358 401 6.34e-12 65.9
MS.gene32719.t1 MTR_3g092780 49.231 65 32 1 219 283 577 640 8.21e-12 65.9
MS.gene32719.t1 MTR_3g092780 49.231 65 32 1 219 283 576 639 8.91e-12 65.9
MS.gene32719.t1 MTR_5g072780 66.667 45 15 0 230 274 172 216 9.30e-12 63.5
MS.gene32719.t1 MTR_3g092780 49.231 65 32 1 219 283 617 680 9.56e-12 65.9
MS.gene32719.t1 MTR_3g092780 49.231 65 32 1 219 283 618 681 9.56e-12 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32719.t1 AT5G24930 52.000 375 80 13 1 289 1 361 6.41e-95 285
MS.gene32719.t1 AT2G24790 54.266 293 95 11 2 277 4 274 4.56e-94 280
MS.gene32719.t1 AT5G57660 42.775 346 131 10 2 289 18 354 2.26e-74 232
MS.gene32719.t1 AT3G02380 40.351 342 141 9 3 289 13 346 4.26e-73 228
MS.gene32719.t1 AT5G15850 41.210 347 143 8 3 289 9 354 5.64e-70 220
MS.gene32719.t1 AT5G15840 39.335 361 134 14 6 289 20 372 1.25e-65 210
MS.gene32719.t1 AT2G24790 56.180 178 55 6 2 171 4 166 7.91e-58 185
MS.gene32719.t1 AT5G15840 41.935 186 82 7 6 166 20 204 2.06e-37 134
MS.gene32719.t1 AT1G49130 28.435 313 176 10 4 280 26 326 1.62e-22 95.5
MS.gene32719.t1 AT1G49130 28.435 313 176 10 4 280 19 319 1.82e-22 95.5
MS.gene32719.t1 AT4G38960 35.570 149 74 3 5 138 4 145 3.34e-20 86.3
MS.gene32719.t1 AT4G38960 35.570 149 74 3 5 138 4 145 3.34e-20 86.3
MS.gene32719.t1 AT2G21320 49.398 83 34 1 5 79 4 86 6.36e-20 85.1
MS.gene32719.t1 AT1G78600 42.017 119 56 3 6 111 5 123 4.88e-19 85.9
MS.gene32719.t1 AT1G78600 42.017 119 56 3 6 111 5 123 5.18e-19 85.5
MS.gene32719.t1 AT2G47890 42.857 91 50 1 4 94 11 99 4.51e-18 82.8
MS.gene32719.t1 AT2G47890 42.857 91 50 1 4 94 11 99 5.04e-18 83.2
MS.gene32719.t1 AT2G47890 27.273 308 161 14 3 276 53 331 1.33e-13 70.5
MS.gene32719.t1 AT2G47890 42.857 91 50 1 4 94 11 99 6.21e-18 81.3
MS.gene32719.t1 AT1G06040 43.750 96 45 1 6 92 5 100 7.24e-18 81.3
MS.gene32719.t1 AT1G06040 43.750 96 45 1 6 92 5 100 9.95e-18 79.3
MS.gene32719.t1 AT1G75540 40.404 99 47 1 6 92 5 103 1.15e-17 82.0
MS.gene32719.t1 AT2G33500 42.697 89 51 0 6 94 12 100 3.19e-17 81.6
MS.gene32719.t1 AT2G33500 52.632 57 27 0 220 276 345 401 1.07e-12 68.2
MS.gene32719.t1 AT2G33500 42.697 89 51 0 6 94 12 100 3.21e-17 81.6
MS.gene32719.t1 AT2G33500 52.632 57 27 0 220 276 344 400 1.10e-12 68.2
MS.gene32719.t1 AT2G31380 34.177 158 87 4 6 153 5 155 1.42e-16 77.8
MS.gene32719.t1 AT1G28050 41.573 89 52 0 6 94 9 97 4.55e-16 78.2
MS.gene32719.t1 AT1G28050 56.000 50 22 0 228 277 381 430 9.12e-11 62.4
MS.gene32719.t1 AT5G48250 38.043 92 55 1 5 94 4 95 1.09e-14 73.9
MS.gene32719.t1 AT3G07650 45.205 73 40 0 5 77 4 76 3.32e-14 72.4
MS.gene32719.t1 AT3G07650 35.185 108 65 2 174 279 258 362 8.63e-11 62.4
MS.gene32719.t1 AT3G07650 45.205 73 40 0 5 77 4 76 3.32e-14 72.4
MS.gene32719.t1 AT3G07650 35.185 108 65 2 174 279 258 362 8.63e-11 62.4
MS.gene32719.t1 AT3G07650 45.205 73 40 0 5 77 4 76 3.32e-14 72.4
MS.gene32719.t1 AT3G07650 35.185 108 65 2 174 279 258 362 8.63e-11 62.4
MS.gene32719.t1 AT3G07650 45.205 73 40 0 5 77 4 76 3.32e-14 72.4
MS.gene32719.t1 AT3G07650 35.185 108 65 2 174 279 258 362 8.63e-11 62.4
MS.gene32719.t1 AT4G10240 33.714 175 81 7 6 165 5 159 4.28e-14 68.9
MS.gene32719.t1 AT4G39070 37.755 98 51 1 5 92 4 101 1.69e-13 68.9
MS.gene32719.t1 AT1G73870 55.556 63 27 1 213 275 327 388 2.48e-13 70.1
MS.gene32719.t1 AT4G15250 40.476 84 50 0 6 89 5 88 4.64e-13 68.9
MS.gene32719.t1 AT1G68190 38.824 85 50 1 4 88 41 123 6.39e-13 68.9
MS.gene32719.t1 AT1G68190 38.824 85 50 1 4 88 12 94 7.62e-13 68.6
MS.gene32719.t1 AT1G68190 38.824 85 50 1 4 88 12 94 7.62e-13 68.6
MS.gene32719.t1 AT1G25440 36.029 136 72 5 158 286 288 415 4.81e-12 66.2
MS.gene32719.t1 AT3G21880 38.202 89 53 1 6 94 5 91 1.27e-11 64.7
MS.gene32719.t1 AT3G21880 38.202 89 53 1 6 94 9 95 1.39e-11 64.7
MS.gene32719.t1 AT3G21880 38.202 89 53 1 6 94 9 95 1.39e-11 64.7
MS.gene32719.t1 AT5G14370 63.043 46 17 0 232 277 294 339 1.92e-11 64.3
MS.gene32719.t1 AT4G25990 60.870 46 18 0 232 277 341 386 2.40e-11 63.9
MS.gene32719.t1 AT1G68520 68.182 44 14 0 232 275 357 400 2.57e-11 63.9
MS.gene32719.t1 AT5G60100 31.217 189 106 6 95 276 342 513 2.95e-11 63.9
MS.gene32719.t1 AT5G60100 31.217 189 106 6 95 276 315 486 3.37e-11 63.9
MS.gene32719.t1 AT5G60100 31.217 189 106 6 95 276 315 486 3.37e-11 63.9
MS.gene32719.t1 AT5G60100 31.217 189 106 6 95 276 315 486 3.37e-11 63.9

Find 71 sgRNAs with CRISPR-Local

Find 74 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATGCAGTTGATTCCATTTC+TGG 0.107062 3.1:+83405079 MS.gene32719:CDS
GAAACTAGGCCTAGGATTAA+AGG 0.280004 3.1:+83405037 MS.gene32719:CDS
CCGCAGAGGAAGGCGGAGTC+AGG 0.296878 3.1:-83404205 None:intergenic
TTCCGAAGCTATTCCGTTCA+TGG 0.298068 3.1:+83404576 MS.gene32719:CDS
AAGCTAGGGTTATGAGGTAT+AGG 0.350898 3.1:+83404951 MS.gene32719:CDS
GACGGTGTTGTTCCCGTTCA+TGG 0.369225 3.1:+83404631 MS.gene32719:CDS
GCAAACCTCCCTTTAATCCT+AGG 0.392261 3.1:-83405046 None:intergenic
CCGTTCATGGATCTCGATTA+CGG 0.395344 3.1:+83404589 MS.gene32719:CDS
TTTGTTAGCGGCGTGGACTT+TGG 0.416071 3.1:-83404239 None:intergenic
GGATCGAAGTTGCCATGAAC+GGG 0.430806 3.1:-83404643 None:intergenic
TGGCGGCGGATCGTGAAGCT+AGG 0.432649 3.1:+83404936 MS.gene32719:CDS
GATTCGAAGAACGGAGTAAC+GGG 0.439450 3.1:-83404415 None:intergenic
AGCTAGGGTTATGAGGTATA+GGG 0.439658 3.1:+83404952 MS.gene32719:CDS
AGATATCGAACTGTGGTTAC+GGG 0.444139 3.1:+83404909 MS.gene32719:CDS
GAGATATCGAACTGTGGTTA+CGG 0.445942 3.1:+83404908 MS.gene32719:CDS
AACGATGCCGTATCCAGAAA+TGG 0.448783 3.1:-83405092 None:intergenic
GTTGATTCCATTTCTGGATA+CGG 0.451790 3.1:+83405085 MS.gene32719:CDS
GATGAGTTGAGATCTGCTTT+CGG 0.461772 3.1:-83404535 None:intergenic
TCCGACACGGTGTTCCCGTC+AGG 0.479338 3.1:-83404887 None:intergenic
TTTAACAGAGTGAGAAGTGT+TGG 0.480554 3.1:-83404437 None:intergenic
TGGTTACGGGACGGTGGCGG+CGG 0.488159 3.1:+83404922 MS.gene32719:CDS
AAACTAGGCCTAGGATTAAA+GGG 0.490422 3.1:+83405038 MS.gene32719:CDS
AGTGTGGATGTCACGGTCAC+AGG 0.492186 3.1:-83404359 None:intergenic
ATCCATGAACGGAATAGCTT+CGG 0.492715 3.1:-83404578 None:intergenic
TTATGAGGTATAGGGAGAAG+AGG 0.496368 3.1:+83404960 MS.gene32719:CDS
TTCTTGTAAGCTGAAACAAA+TGG 0.496560 3.1:-83404664 None:intergenic
TTAGGTATCGTCTTCGTCGA+TGG 0.503016 3.1:+83404850 MS.gene32719:intron
GTAAAGCATATGCGGAAACT+AGG 0.510714 3.1:+83405023 MS.gene32719:CDS
CTGTGGTTACGGGACGGTGG+CGG 0.513006 3.1:+83404919 MS.gene32719:CDS
CGCAGAGGAAGGCGGAGTCA+GGG 0.518288 3.1:-83404204 None:intergenic
TCGTCTTCGTCGATGGAGGT+TGG 0.523017 3.1:+83404857 MS.gene32719:CDS
AGGTTGGAGTTGTTCCTGAC+GGG 0.524585 3.1:+83404873 MS.gene32719:CDS
GGATTCGAAGAACGGAGTAA+CGG 0.536432 3.1:-83404416 None:intergenic
GCTTTACACGTGACATGTGC+AGG 0.538227 3.1:-83404313 None:intergenic
GGCGTGGACTTTGGAGTCAC+AGG 0.543388 3.1:-83404230 None:intergenic
GTCACAGGCGCCGCAGAGGA+AGG 0.545693 3.1:-83404215 None:intergenic
TCGGAATCAGAGAAAAGATA+AGG 0.547155 3.1:-83404559 None:intergenic
TGGATCGAAGTTGCCATGAA+CGG 0.558189 3.1:-83404644 None:intergenic
CGCTCGTGACGTGCGGCGAG+AGG 0.558417 3.1:-83404388 None:intergenic
ATGAAGCTGAAGTTGCTTCG+TGG 0.559796 3.1:+83404500 MS.gene32719:CDS
GCAGTAGAGAGTAGCGGTGG+CGG 0.563031 3.1:-83404179 None:intergenic
GAAGTGTTGGATTCGAAGAA+CGG 0.565969 3.1:-83404424 None:intergenic
GATGCAGAGAGAAGCTGCAT+CGG 0.576026 3.1:-83404335 None:intergenic
CATATGCGGAAACTAGGCCT+AGG 0.577094 3.1:+83405029 MS.gene32719:CDS
GGCGGCGGATCGTGAAGCTA+GGG 0.577274 3.1:+83404937 MS.gene32719:CDS
ACGAGAGGCGAGTTTGTTAG+CGG 0.583367 3.1:-83404251 None:intergenic
GGTATCGTCTTCGTCGATGG+AGG 0.585063 3.1:+83404853 MS.gene32719:intron
ACAGGCGCCGCAGAGGAAGG+CGG 0.586506 3.1:-83404212 None:intergenic
GATTAGCAGTGTGGATGTCA+CGG 0.589251 3.1:-83404366 None:intergenic
CGCTTCTCGTAAAGCATATG+CGG 0.592961 3.1:+83405015 MS.gene32719:CDS
GAGGTTGGAGTTGTTCCTGA+CGG 0.607148 3.1:+83404872 MS.gene32719:CDS
GGGAACACGCTCGTGACGTG+CGG 0.610507 3.1:-83404395 None:intergenic
ATCGTGAAGCTAGGGTTATG+AGG 0.615678 3.1:+83404945 MS.gene32719:CDS
TGGAGTCACAGGCGCCGCAG+AGG 0.616423 3.1:-83404219 None:intergenic
AGGCGAGTTTGTTAGCGGCG+TGG 0.630051 3.1:-83404246 None:intergenic
TGACTAATCTGCGACTGTGA+CGG 0.630286 3.1:-83404721 None:intergenic
AGTGACGCGAGGGTGACGAG+AGG 0.632213 3.1:-83404266 None:intergenic
GCGGCAGTAGAGAGTAGCGG+TGG 0.638763 3.1:-83404182 None:intergenic
AGGGCGGCAGTAGAGAGTAG+CGG 0.646899 3.1:-83404185 None:intergenic
CGGCGAGAGGATTAGCAGTG+TGG 0.659805 3.1:-83404375 None:intergenic
AGAGGAAGGCGGAGTCAGGG+CGG 0.663446 3.1:-83404201 None:intergenic
CCTGACTCCGCCTTCCTCTG+CGG 0.671751 3.1:+83404205 MS.gene32719:CDS
ATCGAACTGTGGTTACGGGA+CGG 0.672790 3.1:+83404913 MS.gene32719:CDS
TCCTGACGGGAACACCGTGT+CGG 0.682213 3.1:+83404886 MS.gene32719:CDS
GAACTGTGGTTACGGGACGG+TGG 0.687113 3.1:+83404916 MS.gene32719:CDS
CTTCGCAGAGAGTGACGCGA+GGG 0.694593 3.1:-83404276 None:intergenic
CTAGGCCTAGGATTAAAGGG+AGG 0.700310 3.1:+83405041 MS.gene32719:CDS
ACTTCGCAGAGAGTGACGCG+AGG 0.710854 3.1:-83404277 None:intergenic
CCGTAATCGAGATCCATGAA+CGG 0.730276 3.1:-83404589 None:intergenic
ACAGTTCGATATCTCCGACA+CGG 0.744977 3.1:-83404900 None:intergenic
GTGTCGGAGATATCGAACTG+TGG 0.760977 3.1:+83404902 MS.gene32719:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTGTTGTGATTAATT+AGG + chr3.1:83404832-83404851 MS.gene32719:intron 15.0%
!!! TAAGTTTGTTTTTATTGACA+TGG + chr3.1:83404773-83404792 MS.gene32719:intron 20.0%
! GTAATTGAACATAAAAACGA+CGG + chr3.1:83404613-83404632 MS.gene32719:CDS 25.0%
TTCTTGTAAGCTGAAACAAA+TGG - chr3.1:83404667-83404686 None:intergenic 30.0%
AAACTAGGCCTAGGATTAAA+GGG + chr3.1:83405038-83405057 MS.gene32719:CDS 35.0%
TCGGAATCAGAGAAAAGATA+AGG - chr3.1:83404562-83404581 None:intergenic 35.0%
! GTTGATTCCATTTCTGGATA+CGG + chr3.1:83405085-83405104 MS.gene32719:CDS 35.0%
! TTTAACAGAGTGAGAAGTGT+TGG - chr3.1:83404440-83404459 None:intergenic 35.0%
AAGCTAGGGTTATGAGGTAT+AGG + chr3.1:83404951-83404970 MS.gene32719:CDS 40.0%
AGATATCGAACTGTGGTTAC+GGG + chr3.1:83404909-83404928 MS.gene32719:CDS 40.0%
AGCTAGGGTTATGAGGTATA+GGG + chr3.1:83404952-83404971 MS.gene32719:CDS 40.0%
ATCCATGAACGGAATAGCTT+CGG - chr3.1:83404581-83404600 None:intergenic 40.0%
GAAACTAGGCCTAGGATTAA+AGG + chr3.1:83405037-83405056 MS.gene32719:CDS 40.0%
GAGATATCGAACTGTGGTTA+CGG + chr3.1:83404908-83404927 MS.gene32719:CDS 40.0%
GTAAAGCATATGCGGAAACT+AGG + chr3.1:83405023-83405042 MS.gene32719:CDS 40.0%
TTATGAGGTATAGGGAGAAG+AGG + chr3.1:83404960-83404979 MS.gene32719:CDS 40.0%
! GATGAGTTGAGATCTGCTTT+CGG - chr3.1:83404538-83404557 None:intergenic 40.0%
! GATGCAGTTGATTCCATTTC+TGG + chr3.1:83405079-83405098 MS.gene32719:CDS 40.0%
!! GAAGTGTTGGATTCGAAGAA+CGG - chr3.1:83404427-83404446 None:intergenic 40.0%
AACGATGCCGTATCCAGAAA+TGG - chr3.1:83405095-83405114 None:intergenic 45.0%
ACAGTTCGATATCTCCGACA+CGG - chr3.1:83404903-83404922 None:intergenic 45.0%
ATCGTGAAGCTAGGGTTATG+AGG + chr3.1:83404945-83404964 MS.gene32719:CDS 45.0%
ATGAAGCTGAAGTTGCTTCG+TGG + chr3.1:83404500-83404519 MS.gene32719:CDS 45.0%
CCGTAATCGAGATCCATGAA+CGG - chr3.1:83404592-83404611 None:intergenic 45.0%
CCGTTCATGGATCTCGATTA+CGG + chr3.1:83404589-83404608 MS.gene32719:CDS 45.0%
CGCTTCTCGTAAAGCATATG+CGG + chr3.1:83405015-83405034 MS.gene32719:CDS 45.0%
GATTAGCAGTGTGGATGTCA+CGG - chr3.1:83404369-83404388 None:intergenic 45.0%
GATTCGAAGAACGGAGTAAC+GGG - chr3.1:83404418-83404437 None:intergenic 45.0%
GCAAACCTCCCTTTAATCCT+AGG - chr3.1:83405049-83405068 None:intergenic 45.0%
GGATTCGAAGAACGGAGTAA+CGG - chr3.1:83404419-83404438 None:intergenic 45.0%
TGACTAATCTGCGACTGTGA+CGG - chr3.1:83404724-83404743 None:intergenic 45.0%
TGGATCGAAGTTGCCATGAA+CGG - chr3.1:83404647-83404666 None:intergenic 45.0%
TTCCGAAGCTATTCCGTTCA+TGG + chr3.1:83404576-83404595 MS.gene32719:CDS 45.0%
! TTAGGTATCGTCTTCGTCGA+TGG + chr3.1:83404850-83404869 MS.gene32719:intron 45.0%
AGGTTGGAGTTGTTCCTGAC+GGG + chr3.1:83404873-83404892 MS.gene32719:CDS 50.0%
ATCGAACTGTGGTTACGGGA+CGG + chr3.1:83404913-83404932 MS.gene32719:CDS 50.0%
CATATGCGGAAACTAGGCCT+AGG + chr3.1:83405029-83405048 MS.gene32719:CDS 50.0%
CTAGGCCTAGGATTAAAGGG+AGG + chr3.1:83405041-83405060 MS.gene32719:CDS 50.0%
GAGGTTGGAGTTGTTCCTGA+CGG + chr3.1:83404872-83404891 MS.gene32719:CDS 50.0%
GATGCAGAGAGAAGCTGCAT+CGG - chr3.1:83404338-83404357 None:intergenic 50.0%
GCTTTACACGTGACATGTGC+AGG - chr3.1:83404316-83404335 None:intergenic 50.0%
GGATCGAAGTTGCCATGAAC+GGG - chr3.1:83404646-83404665 None:intergenic 50.0%
GTGTCGGAGATATCGAACTG+TGG + chr3.1:83404902-83404921 MS.gene32719:CDS 50.0%
TTTGTTAGCGGCGTGGACTT+TGG - chr3.1:83404242-83404261 None:intergenic 50.0%
!! ACGAGAGGCGAGTTTGTTAG+CGG - chr3.1:83404254-83404273 None:intergenic 50.0%
AGTGTGGATGTCACGGTCAC+AGG - chr3.1:83404362-83404381 None:intergenic 55.0%
GGTATCGTCTTCGTCGATGG+AGG + chr3.1:83404853-83404872 MS.gene32719:intron 55.0%
TCGTCTTCGTCGATGGAGGT+TGG + chr3.1:83404857-83404876 MS.gene32719:CDS 55.0%
!! GACGGTGTTGTTCCCGTTCA+TGG + chr3.1:83404631-83404650 MS.gene32719:CDS 55.0%
ACTTCGCAGAGAGTGACGCG+AGG - chr3.1:83404280-83404299 None:intergenic 60.0%
AGGGCGGCAGTAGAGAGTAG+CGG - chr3.1:83404188-83404207 None:intergenic 60.0%
CTTCGCAGAGAGTGACGCGA+GGG - chr3.1:83404279-83404298 None:intergenic 60.0%
GAACTGTGGTTACGGGACGG+TGG + chr3.1:83404916-83404935 MS.gene32719:CDS 60.0%
GCAGTAGAGAGTAGCGGTGG+CGG - chr3.1:83404182-83404201 None:intergenic 60.0%
TCCTGACGGGAACACCGTGT+CGG + chr3.1:83404886-83404905 MS.gene32719:CDS 60.0%
! CGGCGAGAGGATTAGCAGTG+TGG - chr3.1:83404378-83404397 None:intergenic 60.0%
!! AGGCGAGTTTGTTAGCGGCG+TGG - chr3.1:83404249-83404268 None:intergenic 60.0%
!! GGCGTGGACTTTGGAGTCAC+AGG - chr3.1:83404233-83404252 None:intergenic 60.0%
AGAGGAAGGCGGAGTCAGGG+CGG - chr3.1:83404204-83404223 None:intergenic 65.0%
AGTGACGCGAGGGTGACGAG+AGG - chr3.1:83404269-83404288 None:intergenic 65.0%
CCTGACTCCGCCTTCCTCTG+CGG + chr3.1:83404205-83404224 MS.gene32719:CDS 65.0%
CGCAGAGGAAGGCGGAGTCA+GGG - chr3.1:83404207-83404226 None:intergenic 65.0%
CTGTGGTTACGGGACGGTGG+CGG + chr3.1:83404919-83404938 MS.gene32719:CDS 65.0%
GCGGCAGTAGAGAGTAGCGG+TGG - chr3.1:83404185-83404204 None:intergenic 65.0%
GGCGGCGGATCGTGAAGCTA+GGG + chr3.1:83404937-83404956 MS.gene32719:CDS 65.0%
GGGAACACGCTCGTGACGTG+CGG - chr3.1:83404398-83404417 None:intergenic 65.0%
TCCGACACGGTGTTCCCGTC+AGG - chr3.1:83404890-83404909 None:intergenic 65.0%
TGGCGGCGGATCGTGAAGCT+AGG + chr3.1:83404936-83404955 MS.gene32719:CDS 65.0%
CCGCAGAGGAAGGCGGAGTC+AGG - chr3.1:83404208-83404227 None:intergenic 70.0%
! ACAGGCGCCGCAGAGGAAGG+CGG - chr3.1:83404215-83404234 None:intergenic 70.0%
! GTCACAGGCGCCGCAGAGGA+AGG - chr3.1:83404218-83404237 None:intergenic 70.0%
! TGGAGTCACAGGCGCCGCAG+AGG - chr3.1:83404222-83404241 None:intergenic 70.0%
! TGGTTACGGGACGGTGGCGG+CGG + chr3.1:83404922-83404941 MS.gene32719:CDS 70.0%
CGCTCGTGACGTGCGGCGAG+AGG - chr3.1:83404391-83404410 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 83404148 83405126 83404148 ID=MS.gene32719
chr3.1 mRNA 83404148 83405126 83404148 ID=MS.gene32719.t1;Parent=MS.gene32719
chr3.1 exon 83404148 83404747 83404148 ID=MS.gene32719.t1.exon1;Parent=MS.gene32719.t1
chr3.1 CDS 83404148 83404747 83404148 ID=cds.MS.gene32719.t1;Parent=MS.gene32719.t1
chr3.1 exon 83404854 83405126 83404854 ID=MS.gene32719.t1.exon2;Parent=MS.gene32719.t1
chr3.1 CDS 83404854 83405126 83404854 ID=cds.MS.gene32719.t1;Parent=MS.gene32719.t1
Gene Sequence

>MS.gene32719

ATGGCTTCTAAACTCTGTGACTCATGCAAATCCGCCACCGCTACTCTCTACTGCCGCCCTGACTCCGCCTTCCTCTGCGGCGCCTGTGACTCCAAAGTCCACGCCGCTAACAAACTCGCCTCTCGTCACCCTCGCGTCACTCTCTGCGAAGTCTGCGAACAAGCACCTGCACATGTCACGTGTAAAGCCGATGCAGCTTCTCTCTGCATCACCTGTGACCGTGACATCCACACTGCTAATCCTCTCGCCGCACGTCACGAGCGTGTTCCCGTTACTCCGTTCTTCGAATCCAACACTTCTCACTCTGTTAAATCGCTTCAAAACAATAACAACAACTACGATGCTGTTAAAGATGAAGCTGAAGTTGCTTCGTGGTTGATCTCTGATCCGAAAGCAGATCTCAACTCATCTCCTTATCTTTTCTCTGATTCCGAAGCTATTCCGTTCATGGATCTCGATTACGGTGTAATTGAACATAAAAACGACGGTGTTGTTCCCGTTCATGGCAACTTCGATCCATTTGTTTCAGCTTACAAGAACAACAACGTGCACTTGCATACAGAGTTAGAAACACCGTCACAGTCGCAGATTAGTCAGAGTGTAAGAGATCTTAGATCTAAGATTCTAAGTTTGTTTTTATTGACATGGAAATATAATATTGATTAATATATATCTTACACTTATTTTTTTTGTTGTGATTAATTAGGTATCGTCTTCGTCGATGGAGGTTGGAGTTGTTCCTGACGGGAACACCGTGTCGGAGATATCGAACTGTGGTTACGGGACGGTGGCGGCGGATCGTGAAGCTAGGGTTATGAGGTATAGGGAGAAGAGGAAGAATAGAAGATTCGAGAAGACGATTCGTTACGCTTCTCGTAAAGCATATGCGGAAACTAGGCCTAGGATTAAAGGGAGGTTTGCAAAACGCACTGATGCAGTTGATTCCATTTCTGGATACGGCATCGTTCCCACTTGTTGA
Protein sequence

>MS.gene32719.t1

MASKLCDSCKSATATLYCRPDSAFLCGACDSKVHAANKLASRHPRVTLCEVCEQAPAHVTCKADAASLCITCDRDIHTANPLAARHERVPVTPFFESNTSHSVKSLQNNNNNYDAVKDEAEVASWLISDPKADLNSSPYLFSDSEAIPFMDLDYGVIEHKNDGVVPVHGNFDPFVSAYKNNNVHLHTELETPSQSQISQSVSSSSMEVGVVPDGNTVSEISNCGYGTVAADREARVMRYREKRKNRRFEKTIRYASRKAYAETRPRIKGRFAKRTDAVDSISGYGIVPTC