AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene32777

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32777.t1 MTR_3g104830 86.391 507 49 5 1 500 5 498 0.0 869
MS.gene32777.t1 MTR_3g465110 51.978 556 186 14 1 499 10 541 9.25e-159 462
MS.gene32777.t1 MTR_5g024070 45.977 87 42 2 395 478 199 283 5.04e-12 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32777.t1 AT5G16100 32.721 272 128 11 260 484 177 440 4.99e-33 130
MS.gene32777.t1 AT5G66990 27.536 138 76 4 358 483 121 246 7.19e-11 63.5

Find 103 sgRNAs with CRISPR-Local

Find 150 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACCTTTCTGTGGGGCCATC+TGG 0.198703 3.1:+82774472 None:intergenic
TTCCAACTCCATGATGATTT+TGG 0.207331 3.1:+82776072 None:intergenic
TTATTCGTATTGAAGAAAAT+TGG 0.230661 3.1:+82776194 None:intergenic
AATCTTATTCAAAATAATTC+AGG 0.275363 3.1:-82774952 MS.gene32777:intron
GGCAATACAATGGAATTAAA+TGG 0.280054 3.1:+82775951 None:intergenic
ACCACAATGTTTAATCATTT+CGG 0.280881 3.1:+82774258 None:intergenic
CACCATCAGATACCTGTTAA+TGG 0.281140 3.1:-82775277 MS.gene32777:CDS
TTTGAAAAGCTTGAGATTCA+TGG 0.294484 3.1:-82775328 MS.gene32777:CDS
TTGGATTGATGATATTAATA+TGG 0.313839 3.1:-82774977 MS.gene32777:CDS
CTTGGTGGTAGTAATAATAA+TGG 0.330532 3.1:-82775981 MS.gene32777:CDS
TCAGTCCTTGCCCTTGCCTC+TGG 0.332263 3.1:+82774304 None:intergenic
ACCATCAGATACCTGTTAAT+GGG 0.352749 3.1:-82775276 MS.gene32777:CDS
ACTGATGGGGCCCCATTAAC+AGG 0.356308 3.1:+82775265 None:intergenic
CAAACTCAGCATAACAATAA+TGG 0.360613 3.1:-82776101 MS.gene32777:CDS
TGATCTGCTGCTTCATCAAT+AGG 0.368145 3.1:+82774554 None:intergenic
CAAGAGCAAATGGGTGATAA+TGG 0.372132 3.1:-82774768 MS.gene32777:CDS
TACTACGGACTTGGAATACT+TGG 0.382509 3.1:-82775999 MS.gene32777:CDS
CTCTTTGCACTGGATTGGAT+TGG 0.394071 3.1:-82774996 MS.gene32777:CDS
TATTCGTATTGAAGAAAATT+GGG 0.396664 3.1:+82776195 None:intergenic
AAAACGGGAATCTGAATTGC+AGG 0.401372 3.1:+82775549 None:intergenic
GCCCATGTTGAATATATTCA+TGG 0.402375 3.1:+82775793 None:intergenic
TATTGTTATGCTGAGTTTGT+TGG 0.403342 3.1:+82776105 None:intergenic
GTTTAATCATTTCGGTTTGA+AGG 0.403646 3.1:+82774266 None:intergenic
TCAAATTACTAGCAAATCAA+TGG 0.407732 3.1:-82774339 MS.gene32777:CDS
TCTTATGTTTACAGATGAAT+TGG 0.410262 3.1:-82774796 MS.gene32777:intron
TCGGTTTGGTTCAACAAAAC+GGG 0.413527 3.1:+82775534 None:intergenic
TTCGGTTTGGTTCAACAAAA+CGG 0.419751 3.1:+82775533 None:intergenic
GGTACTAAATTGAACATAAA+CGG 0.419806 3.1:+82775828 None:intergenic
GATTCTAAATTGTAAGCAAA+TGG 0.423137 3.1:+82775900 None:intergenic
GTAATTTGATCTGCTTCAAA+AGG 0.424097 3.1:+82774353 None:intergenic
TGAGACTCTTTGCACTGGAT+TGG 0.424222 3.1:-82775001 MS.gene32777:CDS
ACCTTTCTGTGGGGCCATCT+GGG 0.433450 3.1:+82774473 None:intergenic
ATGAACTACCATCCACTTCA+AGG 0.435584 3.1:+82775604 None:intergenic
GAGGCAAGGGCAAGGACTGA+AGG 0.455362 3.1:-82774301 MS.gene32777:CDS
ATTGAAGAAAATTGGGTCAA+TGG 0.457036 3.1:+82776202 None:intergenic
TCATATTGATCTTGAACCAT+TGG 0.458294 3.1:+82775663 None:intergenic
ATTGTTATGCTGAGTTTGTT+GGG 0.459786 3.1:+82776106 None:intergenic
TTGATTAAGTGCTCGCAGTT+CGG 0.465326 3.1:+82775515 None:intergenic
AAGAAAACTTGTCGTAAAGC+AGG 0.469657 3.1:-82774500 MS.gene32777:CDS
GAGGATAAATCTACTAGTGT+TGG 0.470059 3.1:+82776170 None:intergenic
TAAGTGCTCGCAGTTCGGTT+TGG 0.482116 3.1:+82775520 None:intergenic
CAAGAAAATTACTACGGACT+TGG 0.487097 3.1:-82776008 MS.gene32777:CDS
ACTTGTTCTGAGAGGTTCCT+AGG 0.487950 3.1:+82774741 None:intergenic
AGCAAATCAATGGGAAGGAC+AGG 0.491125 3.1:-82774329 MS.gene32777:CDS
ATTGGTGGAGCAAGAGCAAA+TGG 0.500485 3.1:-82774778 MS.gene32777:CDS
CAAATTACTAGCAAATCAAT+GGG 0.500638 3.1:-82774338 MS.gene32777:CDS
AGGCAAGGGCAAGGACTGAA+GGG 0.501249 3.1:-82774300 MS.gene32777:CDS
TATCGCTGCTGCGGCTAAAG+TGG 0.502806 3.1:+82774193 None:intergenic
TGGAATAAATATCCTTGAAG+TGG 0.508517 3.1:-82775616 MS.gene32777:CDS
CACCAAAATCATCATGGAGT+TGG 0.510235 3.1:-82776074 MS.gene32777:CDS
TATGTTTACAGATGAATTGG+TGG 0.515772 3.1:-82774793 MS.gene32777:intron
AGGATAAATCTACTAGTGTT+GGG 0.516442 3.1:+82776171 None:intergenic
TTACCACTCTGGTTCTGTTG+TGG 0.520379 3.1:+82775930 None:intergenic
TCAATCATTTGATAAACTGA+TGG 0.522363 3.1:+82775250 None:intergenic
AAGGACAGGATCCAGAGGCA+AGG 0.525000 3.1:-82774315 MS.gene32777:CDS
GTCTCATAAAATTCAGACAT+TGG 0.526543 3.1:+82775018 None:intergenic
CAATCATTTGATAAACTGAT+GGG 0.528489 3.1:+82775251 None:intergenic
CACCATGAATATATTCAACA+TGG 0.530162 3.1:-82775795 MS.gene32777:CDS
AACTGCGAGCACTTAATCAA+TGG 0.531669 3.1:-82775512 MS.gene32777:CDS
AGGACAGGATCCAGAGGCAA+GGG 0.531756 3.1:-82774314 MS.gene32777:CDS
TACGGACTTGGAATACTTGG+TGG 0.531875 3.1:-82775996 MS.gene32777:CDS
GCTTGAGATTCATGGAAGGT+TGG 0.532570 3.1:-82775320 MS.gene32777:CDS
CTGCGGCTAAAGTGGCAAGA+AGG 0.552292 3.1:+82774201 None:intergenic
ATAAATATCCTTGAAGTGGA+TGG 0.552881 3.1:-82775612 MS.gene32777:CDS
ATGGATCATTATTACCACTC+TGG 0.558050 3.1:+82775919 None:intergenic
AGTTTGTTGGGACGGTGCAG+TGG 0.561780 3.1:+82776118 None:intergenic
ACCCAGATGGCCCCACAGAA+AGG 0.563782 3.1:-82774474 MS.gene32777:intron
CCCCATTAACAGGTATCTGA+TGG 0.567348 3.1:+82775275 None:intergenic
GTAAAGCAGGGTTACCCAGA+TGG 0.568095 3.1:-82774487 MS.gene32777:CDS
TGTGTGGAACGTCATTTAGC+AGG 0.571153 3.1:-82775060 MS.gene32777:CDS
GTCCATGTTGTTGTACATCA+TGG 0.572620 3.1:+82775721 None:intergenic
TTATGCTGAGTTTGTTGGGA+CGG 0.575049 3.1:+82776110 None:intergenic
GGACCCCACCAAAATCATCA+TGG 0.578955 3.1:-82776080 MS.gene32777:CDS
AGAAAACTTGTCGTAAAGCA+GGG 0.586540 3.1:-82774499 MS.gene32777:CDS
TGGTAATACTGATTCATTTG+CGG 0.586782 3.1:-82775766 MS.gene32777:CDS
CGGCTAAAGTGGCAAGAAGG+CGG 0.587716 3.1:+82774204 None:intergenic
CACCATGATGTACAACAACA+TGG 0.591982 3.1:-82775723 MS.gene32777:CDS
AGGATCCAGAGGCAAGGGCA+AGG 0.602162 3.1:-82774309 MS.gene32777:CDS
GAAATGATTAAACATTGTGG+TGG 0.607415 3.1:-82774256 MS.gene32777:CDS
AAATAAGGGGTTACTGTGGG+TGG 0.609956 3.1:+82775489 None:intergenic
CATCATATTCAACAAGTGCA+TGG 0.612023 3.1:-82775864 MS.gene32777:CDS
ACCGAAATGATTAAACATTG+TGG 0.612061 3.1:-82774259 MS.gene32777:CDS
CCATCAGATACCTGTTAATG+GGG 0.617286 3.1:-82775275 MS.gene32777:CDS
AAAAGCTTGAGATTCATGGA+AGG 0.617730 3.1:-82775324 MS.gene32777:CDS
GGCAAGGGCAAGGACTGAAG+GGG 0.618518 3.1:-82774299 MS.gene32777:CDS
TTACTAGCAAATCAATGGGA+AGG 0.619972 3.1:-82774334 MS.gene32777:CDS
CCATCCCAAGAAAATTACTA+CGG 0.628814 3.1:-82776014 MS.gene32777:CDS
CAACAACATGGACATGGACA+TGG 0.630152 3.1:-82775711 MS.gene32777:CDS
AGAGAAATCATACACACTGA+TGG 0.630751 3.1:-82775444 MS.gene32777:intron
ACCATGAATATATTCAACAT+GGG 0.632720 3.1:-82775794 MS.gene32777:CDS
TATATTCAACATGGGCTGAG+TGG 0.633573 3.1:-82775786 MS.gene32777:CDS
GCGATGTCATACAACGCGGT+TGG 0.634750 3.1:+82774226 None:intergenic
GTTTGTTGGGACGGTGCAGT+GGG 0.641780 3.1:+82776119 None:intergenic
ATATTATTACTTGTTCTGAG+AGG 0.643167 3.1:+82774733 None:intergenic
GATGTACAACAACATGGACA+TGG 0.659086 3.1:-82775717 MS.gene32777:CDS
GTTCTGTTGTGGCAATACAA+TGG 0.661300 3.1:+82775941 None:intergenic
ATGGGAAGGACAGGATCCAG+AGG 0.682760 3.1:-82774320 MS.gene32777:CDS
AATCATTTGATAAACTGATG+GGG 0.687280 3.1:+82775252 None:intergenic
TTGGTGGAGCAAGAGCAAAT+GGG 0.694143 3.1:-82774777 MS.gene32777:CDS
CATATTCAACAAGTGCATGG+TGG 0.702624 3.1:-82775861 MS.gene32777:CDS
TGGGTGATAATGGAACGCCT+AGG 0.709329 3.1:-82774758 MS.gene32777:CDS
TTGCCACAACAGAACCAGAG+TGG 0.723246 3.1:-82775933 MS.gene32777:CDS
GGCGGCGATGTCATACAACG+CGG 0.812023 3.1:+82774222 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AACAATAATAATTTTTCATT+TGG - chr3.1:82774813-82774832 MS.gene32777:intron 10.0%
!! AATCTTATTCAAAATAATTC+AGG - chr3.1:82775497-82775516 MS.gene32777:CDS 15.0%
!! ATATCAAAATAATAGAAACA+TGG + chr3.1:82775068-82775087 None:intergenic 15.0%
!! TATCAAAATAATAGAAACAT+GGG + chr3.1:82775067-82775086 None:intergenic 15.0%
!!! ATTGTTGTTATTTTTATCAA+TGG + chr3.1:82774304-82774323 None:intergenic 15.0%
!! GATAAAAATAACAACAATGT+GGG - chr3.1:82774306-82774325 MS.gene32777:CDS 20.0%
!! TATTCGTATTGAAGAAAATT+GGG + chr3.1:82774257-82774276 None:intergenic 20.0%
!! TGATAAAAATAACAACAATG+TGG - chr3.1:82774305-82774324 MS.gene32777:CDS 20.0%
!! TTATTCGTATTGAAGAAAAT+TGG + chr3.1:82774258-82774277 None:intergenic 20.0%
!!! AAAAATTCTTTATTTCACCA+AGG + chr3.1:82775358-82775377 None:intergenic 20.0%
!!! TGGTGAAATAAAGAATTTTT+TGG - chr3.1:82775358-82775377 MS.gene32777:intron 20.0%
!!! TTGGATTGATGATATTAATA+TGG - chr3.1:82775472-82775491 MS.gene32777:CDS 20.0%
! AATCATTTGATAAACTGATG+GGG + chr3.1:82775200-82775219 None:intergenic 25.0%
! ACCACAATGTTTAATCATTT+CGG + chr3.1:82776194-82776213 None:intergenic 25.0%
! ACCATGAATATATTCAACAT+GGG - chr3.1:82774655-82774674 MS.gene32777:intron 25.0%
! CAAATTACTAGCAAATCAAT+GGG - chr3.1:82776111-82776130 MS.gene32777:CDS 25.0%
! CAATCATTTGATAAACTGAT+GGG + chr3.1:82775201-82775220 None:intergenic 25.0%
! GATTCTAAATTGTAAGCAAA+TGG + chr3.1:82774552-82774571 None:intergenic 25.0%
! GGTACTAAATTGAACATAAA+CGG + chr3.1:82774624-82774643 None:intergenic 25.0%
! TCAAATTACTAGCAAATCAA+TGG - chr3.1:82776110-82776129 MS.gene32777:CDS 25.0%
! TCAATCATTTGATAAACTGA+TGG + chr3.1:82775202-82775221 None:intergenic 25.0%
! TCTTATGTTTACAGATGAAT+TGG - chr3.1:82775653-82775672 MS.gene32777:CDS 25.0%
! TCTTGTGTGTTATACTTATT+TGG - chr3.1:82776008-82776027 MS.gene32777:CDS 25.0%
!! AACTTTTGTAACAGTTTTTG+TGG - chr3.1:82775326-82775345 MS.gene32777:CDS 25.0%
!! ACTTTTGTAACAGTTTTTGT+GGG - chr3.1:82775327-82775346 MS.gene32777:CDS 25.0%
!! ATATATTCATGGTGATTTTC+TGG + chr3.1:82774648-82774667 None:intergenic 25.0%
!! ATATTATTACTTGTTCTGAG+AGG + chr3.1:82775719-82775738 None:intergenic 25.0%
!! TGAATATGATGATCATTTTC+TGG + chr3.1:82774576-82774595 None:intergenic 25.0%
!!! GTTGTTATTTTTATCAATGG+AGG + chr3.1:82774301-82774320 None:intergenic 25.0%
ACAACAACTGCAAAAATAAG+GGG + chr3.1:82774976-82774995 None:intergenic 30.0%
ACCGAAATGATTAAACATTG+TGG - chr3.1:82776190-82776209 MS.gene32777:CDS 30.0%
AGACACGTTATATTAGCAAA+AGG + chr3.1:82776053-82776072 None:intergenic 30.0%
AGGATAAATCTACTAGTGTT+GGG + chr3.1:82774281-82774300 None:intergenic 30.0%
ATAAATATCCTTGAAGTGGA+TGG - chr3.1:82774837-82774856 MS.gene32777:intron 30.0%
ATTGAAGAAAATTGGGTCAA+TGG + chr3.1:82774250-82774269 None:intergenic 30.0%
CAAACTCAGCATAACAATAA+TGG - chr3.1:82774348-82774367 MS.gene32777:CDS 30.0%
CACCATGAATATATTCAACA+TGG - chr3.1:82774654-82774673 MS.gene32777:intron 30.0%
GAAATGATTAAACATTGTGG+TGG - chr3.1:82776193-82776212 MS.gene32777:CDS 30.0%
GACAACAACTGCAAAAATAA+GGG + chr3.1:82774977-82774996 None:intergenic 30.0%
GGCAATACAATGGAATTAAA+TGG + chr3.1:82774501-82774520 None:intergenic 30.0%
GTAATTTGATCTGCTTCAAA+AGG + chr3.1:82776099-82776118 None:intergenic 30.0%
GTACTATACATACTAAGACT+TGG + chr3.1:82775232-82775251 None:intergenic 30.0%
GTCTCATAAAATTCAGACAT+TGG + chr3.1:82775434-82775453 None:intergenic 30.0%
TATGTTTACAGATGAATTGG+TGG - chr3.1:82775656-82775675 MS.gene32777:CDS 30.0%
TATTGTTATGCTGAGTTTGT+TGG + chr3.1:82774347-82774366 None:intergenic 30.0%
TCATATTGATCTTGAACCAT+TGG + chr3.1:82774789-82774808 None:intergenic 30.0%
TGACAACAACTGCAAAAATA+AGG + chr3.1:82774978-82774997 None:intergenic 30.0%
TGGAATAAATATCCTTGAAG+TGG - chr3.1:82774833-82774852 MS.gene32777:intron 30.0%
TTCCTCTGTTCAATACAATA+GGG + chr3.1:82775826-82775845 None:intergenic 30.0%
TTTCCTCTGTTCAATACAAT+AGG + chr3.1:82775827-82775846 None:intergenic 30.0%
TTTGAAAAGCTTGAGATTCA+TGG - chr3.1:82775121-82775140 MS.gene32777:intron 30.0%
! AAAATAATTCAGGTTTGTCC+TGG - chr3.1:82775507-82775526 MS.gene32777:CDS 30.0%
! AAAGACTTTTACCTTTCTGT+GGG + chr3.1:82775989-82776008 None:intergenic 30.0%
! AAGTCCGTAGTAATTTTCTT+GGG + chr3.1:82774442-82774461 None:intergenic 30.0%
! ATTGTTATGCTGAGTTTGTT+GGG + chr3.1:82774346-82774365 None:intergenic 30.0%
! CTTGGTGGTAGTAATAATAA+TGG - chr3.1:82774468-82774487 MS.gene32777:intron 30.0%
! CTTTTCATTCATGTGACATT+GGG - chr3.1:82775615-82775634 MS.gene32777:CDS 30.0%
! GTTTAATCATTTCGGTTTGA+AGG + chr3.1:82776186-82776205 None:intergenic 30.0%
! TCTTTTCATTCATGTGACAT+TGG - chr3.1:82775614-82775633 MS.gene32777:CDS 30.0%
! TGAAACATTTTCTGTTCCAA+TGG - chr3.1:82774770-82774789 MS.gene32777:CDS 30.0%
!! TGGTAATACTGATTCATTTG+CGG - chr3.1:82774683-82774702 MS.gene32777:intron 30.0%
AAAAGCTTGAGATTCATGGA+AGG - chr3.1:82775125-82775144 MS.gene32777:intron 35.0%
AAGAAAACTTGTCGTAAAGC+AGG - chr3.1:82775949-82775968 MS.gene32777:CDS 35.0%
ACCATCAGATACCTGTTAAT+GGG - chr3.1:82775173-82775192 MS.gene32777:intron 35.0%
AGAAAACTTGTCGTAAAGCA+GGG - chr3.1:82775950-82775969 MS.gene32777:CDS 35.0%
AGAGAAATCATACACACTGA+TGG - chr3.1:82775005-82775024 MS.gene32777:CDS 35.0%
ATGGATCATTATTACCACTC+TGG + chr3.1:82774533-82774552 None:intergenic 35.0%
CAAAAATAAGGGGTTACTGT+GGG + chr3.1:82774966-82774985 None:intergenic 35.0%
CAAGAAAATTACTACGGACT+TGG - chr3.1:82774441-82774460 MS.gene32777:intron 35.0%
CATCATATTCAACAAGTGCA+TGG - chr3.1:82774585-82774604 MS.gene32777:CDS 35.0%
CCATCCCAAGAAAATTACTA+CGG - chr3.1:82774435-82774454 MS.gene32777:intron 35.0%
CGTGTCTTAAAATTAACGAC+AGG - chr3.1:82776066-82776085 MS.gene32777:CDS 35.0%
GAGGATAAATCTACTAGTGT+TGG + chr3.1:82774282-82774301 None:intergenic 35.0%
GCCCATGTTGAATATATTCA+TGG + chr3.1:82774659-82774678 None:intergenic 35.0%
TACCCTATTGTATTGAACAG+AGG - chr3.1:82775821-82775840 MS.gene32777:CDS 35.0%
TTACTAGCAAATCAATGGGA+AGG - chr3.1:82776115-82776134 MS.gene32777:CDS 35.0%
! AAGACTTTTACCTTTCTGTG+GGG + chr3.1:82775988-82776007 None:intergenic 35.0%
! CAAGTCCGTAGTAATTTTCT+TGG + chr3.1:82774443-82774462 None:intergenic 35.0%
! CAAGTTTTCTTGAGAACTGT+TGG + chr3.1:82775940-82775959 None:intergenic 35.0%
! GAAAGACTTTTACCTTTCTG+TGG + chr3.1:82775990-82776009 None:intergenic 35.0%
! TAGTAATTTTCTTGGGATGG+TGG + chr3.1:82774435-82774454 None:intergenic 35.0%
! TATTCATGGTGATTTTCTGG+CGG + chr3.1:82774645-82774664 None:intergenic 35.0%
! TTCCAACTCCATGATGATTT+TGG + chr3.1:82774380-82774399 None:intergenic 35.0%
! TTTTATGAGACTCTTTGCAC+TGG - chr3.1:82775443-82775462 MS.gene32777:intron 35.0%
!! AACTCCATGATGATTTTGGT+GGG + chr3.1:82774376-82774395 None:intergenic 35.0%
!! TTCGGTTTGGTTCAACAAAA+CGG + chr3.1:82774919-82774938 None:intergenic 35.0%
AAAACGGGAATCTGAATTGC+AGG + chr3.1:82774903-82774922 None:intergenic 40.0%
AACTGCGAGCACTTAATCAA+TGG - chr3.1:82774937-82774956 MS.gene32777:intron 40.0%
ATGAACTACCATCCACTTCA+AGG + chr3.1:82774848-82774867 None:intergenic 40.0%
CAAGAGCAAATGGGTGATAA+TGG - chr3.1:82775681-82775700 MS.gene32777:CDS 40.0%
CACCAAAATCATCATGGAGT+TGG - chr3.1:82774375-82774394 MS.gene32777:intron 40.0%
CACCATCAGATACCTGTTAA+TGG - chr3.1:82775172-82775191 MS.gene32777:intron 40.0%
CACCATGATGTACAACAACA+TGG - chr3.1:82774726-82774745 MS.gene32777:intron 40.0%
CATATTCAACAAGTGCATGG+TGG - chr3.1:82774588-82774607 MS.gene32777:CDS 40.0%
CCATCAGATACCTGTTAATG+GGG - chr3.1:82775174-82775193 MS.gene32777:intron 40.0%
GACATTGGGTTAAGTTTGAG+TGG - chr3.1:82775629-82775648 MS.gene32777:CDS 40.0%
GATGTACAACAACATGGACA+TGG - chr3.1:82774732-82774751 MS.gene32777:intron 40.0%
GCAAAAATAAGGGGTTACTG+TGG + chr3.1:82774967-82774986 None:intergenic 40.0%
GTCCATGTTGTTGTACATCA+TGG + chr3.1:82774731-82774750 None:intergenic 40.0%
GTTCTGTTGTGGCAATACAA+TGG + chr3.1:82774511-82774530 None:intergenic 40.0%
TACTACGGACTTGGAATACT+TGG - chr3.1:82774450-82774469 MS.gene32777:intron 40.0%
TTGATTAAGTGCTCGCAGTT+CGG + chr3.1:82774937-82774956 None:intergenic 40.0%
! AGTTTTTGTGGGAAAAGCCT+TGG - chr3.1:82775338-82775357 MS.gene32777:CDS 40.0%
! CAACTCCATGATGATTTTGG+TGG + chr3.1:82774377-82774396 None:intergenic 40.0%
! CCGTAGTAATTTTCTTGGGA+TGG + chr3.1:82774438-82774457 None:intergenic 40.0%
! TATATTCAACATGGGCTGAG+TGG - chr3.1:82774663-82774682 MS.gene32777:intron 40.0%
! TGATCTGCTGCTTCATCAAT+AGG + chr3.1:82775898-82775917 None:intergenic 40.0%
!! ACTCCATGATGATTTTGGTG+GGG + chr3.1:82774375-82774394 None:intergenic 40.0%
!! TCGGTTTGGTTCAACAAAAC+GGG + chr3.1:82774918-82774937 None:intergenic 40.0%
!! TTATGCTGAGTTTGTTGGGA+CGG + chr3.1:82774342-82774361 None:intergenic 40.0%
!!! TTTTTTGGCACAGTACTGTG+TGG - chr3.1:82775373-82775392 MS.gene32777:intron 40.0%
AAATAAGGGGTTACTGTGGG+TGG + chr3.1:82774963-82774982 None:intergenic 45.0%
AGCAAATCAATGGGAAGGAC+AGG - chr3.1:82776120-82776139 MS.gene32777:CDS 45.0%
ATTGGTGGAGCAAGAGCAAA+TGG - chr3.1:82775671-82775690 MS.gene32777:CDS 45.0%
CAACAACATGGACATGGACA+TGG - chr3.1:82774738-82774757 MS.gene32777:intron 45.0%
CCCCATTAACAGGTATCTGA+TGG + chr3.1:82775177-82775196 None:intergenic 45.0%
GCTTGAGATTCATGGAAGGT+TGG - chr3.1:82775129-82775148 MS.gene32777:intron 45.0%
GTTTGTCCTGGAAATCACGA+CGG - chr3.1:82775519-82775538 MS.gene32777:CDS 45.0%
TACGGACTTGGAATACTTGG+TGG - chr3.1:82774453-82774472 MS.gene32777:intron 45.0%
TGAGACTCTTTGCACTGGAT+TGG - chr3.1:82775448-82775467 MS.gene32777:CDS 45.0%
TGTGTGGAACGTCATTTAGC+AGG - chr3.1:82775389-82775408 MS.gene32777:intron 45.0%
TTGGTGGAGCAAGAGCAAAT+GGG - chr3.1:82775672-82775691 MS.gene32777:CDS 45.0%
! CTCTTTGCACTGGATTGGAT+TGG - chr3.1:82775453-82775472 MS.gene32777:CDS 45.0%
!! ACTTGTTCTGAGAGGTTCCT+AGG + chr3.1:82775711-82775730 None:intergenic 45.0%
!! TTACCACTCTGGTTCTGTTG+TGG + chr3.1:82774522-82774541 None:intergenic 45.0%
!!! ATTTTCTTGGGATGGTGGAG+TGG + chr3.1:82774430-82774449 None:intergenic 45.0%
!! TATTTATTTGAATTATTTAT+AGG - chr3.1:82775091-82775110 MS.gene32777:CDS 5.0%
CCTGGAAATCACGACGGAAA+AGG - chr3.1:82775525-82775544 MS.gene32777:CDS 50.0%
GGACCCCACCAAAATCATCA+TGG - chr3.1:82774369-82774388 MS.gene32777:intron 50.0%
GTAAAGCAGGGTTACCCAGA+TGG - chr3.1:82775962-82775981 MS.gene32777:CDS 50.0%
TAAGTGCTCGCAGTTCGGTT+TGG + chr3.1:82774932-82774951 None:intergenic 50.0%
TTGCCACAACAGAACCAGAG+TGG - chr3.1:82774516-82774535 MS.gene32777:CDS 50.0%
! CCTTTTCCGTCGTGATTTCC+AGG + chr3.1:82775528-82775547 None:intergenic 50.0%
!! TGGGTGATAATGGAACGCCT+AGG - chr3.1:82775691-82775710 MS.gene32777:CDS 50.0%
AAGGACAGGATCCAGAGGCA+AGG - chr3.1:82776134-82776153 MS.gene32777:CDS 55.0%
ACCTTTCTGTGGGGCCATCT+GGG + chr3.1:82775979-82775998 None:intergenic 55.0%
ACTGATGGGGCCCCATTAAC+AGG + chr3.1:82775187-82775206 None:intergenic 55.0%
AGGACAGGATCCAGAGGCAA+GGG - chr3.1:82776135-82776154 MS.gene32777:CDS 55.0%
AGGCAAGGGCAAGGACTGAA+GGG - chr3.1:82776149-82776168 MS.gene32777:CDS 55.0%
ATGGGAAGGACAGGATCCAG+AGG - chr3.1:82776129-82776148 MS.gene32777:CDS 55.0%
GCGATGTCATACAACGCGGT+TGG + chr3.1:82776226-82776245 None:intergenic 55.0%
TACCTTTCTGTGGGGCCATC+TGG + chr3.1:82775980-82775999 None:intergenic 55.0%
! GTTTGTTGGGACGGTGCAGT+GGG + chr3.1:82774333-82774352 None:intergenic 55.0%
!! AGTTTGTTGGGACGGTGCAG+TGG + chr3.1:82774334-82774353 None:intergenic 55.0%
ACCCAGATGGCCCCACAGAA+AGG - chr3.1:82775975-82775994 MS.gene32777:CDS 60.0%
AGGATCCAGAGGCAAGGGCA+AGG - chr3.1:82776140-82776159 MS.gene32777:CDS 60.0%
GAGGCAAGGGCAAGGACTGA+AGG - chr3.1:82776148-82776167 MS.gene32777:CDS 60.0%
GGCAAGGGCAAGGACTGAAG+GGG - chr3.1:82776150-82776169 MS.gene32777:CDS 60.0%
GGCGGCGATGTCATACAACG+CGG + chr3.1:82776230-82776249 None:intergenic 60.0%
TCAGTCCTTGCCCTTGCCTC+TGG + chr3.1:82776148-82776167 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 82774207 82776264 82774207 ID=MS.gene32777
chr3.1 mRNA 82774207 82776264 82774207 ID=MS.gene32777.t1;Parent=MS.gene32777
chr3.1 exon 82775445 82776264 82775445 ID=MS.gene32777.t1.exon1;Parent=MS.gene32777.t1
chr3.1 CDS 82775445 82776264 82775445 ID=cds.MS.gene32777.t1;Parent=MS.gene32777.t1
chr3.1 exon 82775250 82775358 82775250 ID=MS.gene32777.t1.exon2;Parent=MS.gene32777.t1
chr3.1 CDS 82775250 82775358 82775250 ID=cds.MS.gene32777.t1;Parent=MS.gene32777.t1
chr3.1 exon 82774953 82775131 82774953 ID=MS.gene32777.t1.exon3;Parent=MS.gene32777.t1
chr3.1 CDS 82774953 82775131 82774953 ID=cds.MS.gene32777.t1;Parent=MS.gene32777.t1
chr3.1 exon 82774743 82774804 82774743 ID=MS.gene32777.t1.exon4;Parent=MS.gene32777.t1
chr3.1 CDS 82774743 82774804 82774743 ID=cds.MS.gene32777.t1;Parent=MS.gene32777.t1
chr3.1 exon 82774475 82774630 82774475 ID=MS.gene32777.t1.exon5;Parent=MS.gene32777.t1
chr3.1 CDS 82774475 82774630 82774475 ID=cds.MS.gene32777.t1;Parent=MS.gene32777.t1
chr3.1 exon 82774207 82774383 82774207 ID=MS.gene32777.t1.exon6;Parent=MS.gene32777.t1
chr3.1 CDS 82774207 82774383 82774207 ID=cds.MS.gene32777.t1;Parent=MS.gene32777.t1
Gene Sequence

>MS.gene32777

ATGGAAGATGAAGATCTCATGTTTGACTGCTTCGAAAATTCCATTGACCCAATTTTCTTCAATACGAATAACCCAACACTAGTAGATTTATCCTCCATTGATAAAAATAACAACAATGTGGGTCCCACTGCACCGTCCCAACAAACTCAGCATAACAATAATGGACCCCACCAAAATCATCATGGAGTTGGAATAGTTAGTAATTTTAATGATCTTTTTTCCACTCCACCATCCCAAGAAAATTACTACGGACTTGGAATACTTGGTGGTAGTAATAATAATGGAAATGATCCATTTAATTCCATTGTATTGCCACAACAGAACCAGAGTGGTAATAATGATCCATTTGCTTACAATTTAGAATCACCAGAAAATGATCATCATATTCAACAAGTGCATGGTGGTAGTAATGATCCGTTTATGTTCAATTTAGTACCGCCAGAAAATCACCATGAATATATTCAACATGGGCTGAGTGGTAATACTGATTCATTTGCGGATGTTATGTTACAACAAAATCACCATGATGTACAACAACATGGACATGGACATGGTAGTACAAATGAAACATTTTCTGTTCCAATGGTTCAAGATCAATATGATCAAAACAATAATAATTTTTCATTTGGAATAAATATCCTTGAAGTGGATGGTAGTTCATCAAGAATGTATGAGAATCAACAACAAAGTATACCTGCAATTCAGATTCCCGTTTTGTTGAACCAAACCGAACTGCGAGCACTTAATCAATGGCCACCCACAGTAACCCCTTATTTTTGCAGTTGTTGTCAAGTTCTCAGAGAAATCATACACACTGATGGTTAGTTTTTAATTAATTAATGAATATGAAATTAATGCCCATGTTTCTATTATTTTGATATATATATTTATTTGAATTATTTATAGGTATGCAATTTGAAAAGCTTGAGATTCATGGAAGGTTGGCGTTGATTACACATGCAATTCACCATCAGATACCTGTTAATGGGGCCCCATCAGTTTATCAAATGATTGAGTATGTTCCAAGTCTTAGTATGTATAGTACTATATTAATTTCTTTAATATGTTTTTTTGTGATTTTAAAATTGTCACATTTGAATAATAATAATAACAATTGAAAACTTTTGTAACAGTTTTTGTGGGAAAAGCCTTGGTGAAATAAAGAATTTTTTGGCACAGTACTGTGTGGAACGTCATTTAGCAGGATACATTCTTATGCAAGATCCAATGTCTGAATTTTATGAGACTCTTTGCACTGGATTGGATTGGATTGATGATATTAATATGGAGAATCTTATTCAAAATAATTCAGGTTTGTCCTGGAAATCACGACGGAAAAGGAAAAAAAGTACTTTTGTATTTAGTAATTGTATTTGTATTTATTTAATCAACAATATCATTTTTATTTCTTTTCATTCATGTGACATTGGGTTAAGTTTGAGTGGTTCTTATGTTTACAGATGAATTGGTGGAGCAAGAGCAAATGGGTGATAATGGAACGCCTAGGAACCTCTCAGAACAAGTAATAATATATACTGCTCTTATGTTTTCTTAAACAATAAATAATGAATATATCGTAGTTGCATGCATGTTATGTAACTTATGTTATTGATATACCCTATTGTATTGAACAGAGGAAAAAATCTGCAAAACTGAAATTGAGTGATTTATGTGATTACTTTCATCTACCTATTGATGAAGCAGCAGATCATGAGAAAGTGAAGTTATGTCCAACAGTTCTCAAGAAAACTTGTCGTAAAGCAGGGTTACCCAGATGGCCCCACAGAAAGGTAAAAGTCTTTCTTGTGTGTTATACTTATTTGGTACTGTCAAATGAATATTTCCTTTTGCTAATATAACGTGTCTTAAAATTAACGACAGGTGAAAAGCCTTTTGAAGCAGATCAAATTACTAGCAAATCAATGGGAAGGACAGGATCCAGAGGCAAGGGCAAGGACTGAAGGGGAAATTAAAAGCCTTCAAACCGAAATGATTAAACATTGTGGTGGACATATACCAACCGCGTTGTATGACATCGCCGCCTTCTTGCCACTTTAG
Protein sequence

>MS.gene32777.t1

MEDEDLMFDCFENSIDPIFFNTNNPTLVDLSSIDKNNNNVGPTAPSQQTQHNNNGPHQNHHGVGIVSNFNDLFSTPPSQENYYGLGILGGSNNNGNDPFNSIVLPQQNQSGNNDPFAYNLESPENDHHIQQVHGGSNDPFMFNLVPPENHHEYIQHGLSGNTDSFADVMLQQNHHDVQQHGHGHGSTNETFSVPMVQDQYDQNNNNFSFGINILEVDGSSSRMYENQQQSIPAIQIPVLLNQTELRALNQWPPTVTPYFCSCCQVLREIIHTDGMQFEKLEIHGRLALITHAIHHQIPVNGAPSVYQMIDFCGKSLGEIKNFLAQYCVERHLAGYILMQDPMSEFYETLCTGLDWIDDINMENLIQNNSDELVEQEQMGDNGTPRNLSEQRKKSAKLKLSDLCDYFHLPIDEAADHEKVKLCPTVLKKTCRKAGLPRWPHRKVKSLLKQIKLLANQWEGQDPEARARTEGEIKSLQTEMIKHCGGHIPTALYDIAAFLPL