AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene32841

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32841.t1 MTR_3g103590 84.488 303 28 5 1 294 12 304 1.01e-155 437
MS.gene32841.t1 MTR_1g017090 49.829 293 121 11 6 290 11 285 2.24e-74 229
MS.gene32841.t1 MTR_5g014890 60.123 163 58 4 124 286 114 269 3.95e-57 185
MS.gene32841.t1 MTR_7g093430 69.048 126 36 2 123 248 150 272 4.48e-54 178
MS.gene32841.t1 MTR_1g054285 50.249 201 90 4 50 250 78 268 2.37e-53 176
MS.gene32841.t1 MTR_4g097600 65.354 127 41 2 122 248 116 239 9.60e-52 171
MS.gene32841.t1 MTR_2g061030 65.116 129 42 2 124 252 191 316 5.55e-51 171
MS.gene32841.t1 MTR_4g100550 54.305 151 65 2 123 272 110 257 1.61e-48 163
MS.gene32841.t1 MTR_5g013010 56.944 144 58 2 123 265 126 266 2.22e-48 163
MS.gene32841.t1 MTR_8g006705 67.521 117 34 2 132 247 145 258 1.14e-47 161
MS.gene32841.t1 MTR_4g084750 43.386 189 97 3 62 247 1 182 4.45e-42 144
MS.gene32841.t1 MTR_2g038625 49.102 167 74 4 104 264 50 211 2.43e-39 137
MS.gene32841.t1 MTR_2g038580 49.102 167 74 4 104 264 50 211 2.43e-39 137
MS.gene32841.t1 MTR_4g126900 56.557 122 36 4 135 247 90 203 3.39e-32 119
MS.gene32841.t1 MTR_5g013010 69.355 62 19 0 123 184 126 187 9.87e-22 90.9
MS.gene32841.t1 MTR_1g061660 43.820 89 50 0 133 221 59 147 1.51e-18 82.8
MS.gene32841.t1 MTR_6g007647 48.352 91 47 0 130 220 30 120 2.00e-18 83.2
MS.gene32841.t1 MTR_6g007647 48.352 91 47 0 130 220 64 154 2.40e-18 83.6
MS.gene32841.t1 MTR_5g039000 40.351 114 58 2 121 224 62 175 4.78e-17 79.7
MS.gene32841.t1 MTR_7g010020 40.952 105 55 1 123 220 70 174 6.45e-17 79.3
MS.gene32841.t1 MTR_5g038280 45.745 94 44 1 133 219 87 180 1.92e-16 78.6
MS.gene32841.t1 MTR_7g103340 39.604 101 60 1 121 220 50 150 2.04e-16 77.0
MS.gene32841.t1 MTR_4g107650 37.879 132 77 1 89 220 56 182 3.69e-16 77.8
MS.gene32841.t1 MTR_8g469430 46.809 94 43 1 133 219 89 182 4.81e-16 77.0
MS.gene32841.t1 MTR_8g468210 40.000 100 57 1 124 220 65 164 8.52e-16 76.3
MS.gene32841.t1 MTR_3g086790 45.361 97 46 1 131 220 58 154 8.73e-16 76.6
MS.gene32841.t1 MTR_3g080100 44.211 95 46 1 133 220 20 114 6.58e-15 72.4
MS.gene32841.t1 MTR_3g080100 36.567 134 72 3 133 259 59 186 7.12e-15 73.2
MS.gene32841.t1 MTR_6g011610 44.681 94 45 1 133 219 96 189 8.15e-15 73.2
MS.gene32841.t1 MTR_8g089895 40.909 110 61 1 121 226 42 151 1.30e-14 73.2
MS.gene32841.t1 MTR_6g011610 44.681 94 45 1 133 219 77 170 1.46e-14 72.4
MS.gene32841.t1 MTR_8g026960 37.000 100 60 1 137 236 38 134 8.93e-13 67.0
MS.gene32841.t1 MTR_3g092150 43.529 85 48 0 136 220 41 125 4.54e-12 65.1
MS.gene32841.t1 MTR_5g019650 43.158 95 49 1 123 217 48 137 9.28e-12 63.9
MS.gene32841.t1 MTR_5g019680 39.796 98 59 0 129 226 49 146 5.27e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32841.t1 AT5G06710 58.182 165 61 4 89 253 151 307 2.39e-54 180
MS.gene32841.t1 AT4G37790 68.504 127 37 2 122 248 115 238 2.19e-53 176
MS.gene32841.t1 AT2G22800 68.000 125 37 2 124 248 104 225 2.06e-51 170
MS.gene32841.t1 AT4G16780 40.769 260 111 4 42 266 9 260 6.99e-51 169
MS.gene32841.t1 AT3G60390 64.286 126 43 1 122 247 151 274 1.04e-48 164
MS.gene32841.t1 AT4G17460 61.600 125 46 1 124 248 126 248 1.96e-46 157
MS.gene32841.t1 AT5G47370 60.630 127 48 1 122 248 119 243 3.05e-46 157
MS.gene32841.t1 AT2G44910 64.103 117 40 1 132 248 162 276 2.97e-45 155
MS.gene32841.t1 AT2G01430 55.639 133 48 3 122 248 122 249 9.15e-41 143
MS.gene32841.t1 AT1G70920 50.388 129 53 2 121 248 56 174 2.00e-35 127
MS.gene32841.t1 AT1G70920 46.939 147 66 3 121 266 26 161 2.02e-35 126
MS.gene32841.t1 AT5G06710 57.292 96 36 2 89 184 151 241 5.98e-24 98.6
MS.gene32841.t1 AT2G01430 62.319 69 20 2 122 184 122 190 3.47e-19 84.3
MS.gene32841.t1 AT3G01470 54.167 72 33 0 133 204 68 139 1.78e-16 78.2
MS.gene32841.t1 AT1G69780 40.541 111 56 2 124 224 73 183 5.80e-16 77.0
MS.gene32841.t1 AT5G03790 40.000 95 57 0 127 221 71 165 5.91e-16 75.9
MS.gene32841.t1 AT2G18550 46.154 91 49 0 129 219 57 147 3.67e-15 73.6
MS.gene32841.t1 AT5G15150 40.741 108 52 2 124 219 101 208 4.56e-15 74.7
MS.gene32841.t1 AT2G36610 35.714 126 66 3 109 221 44 167 6.10e-15 72.0
MS.gene32841.t1 AT1G27045 51.389 72 35 0 133 204 68 139 1.27e-14 72.0
MS.gene32841.t1 AT1G27045 51.389 72 35 0 133 204 25 96 1.28e-14 71.2
MS.gene32841.t1 AT1G27045 51.389 72 35 0 133 204 25 96 1.28e-14 71.2
MS.gene32841.t1 AT1G27045 51.389 72 35 0 133 204 17 88 1.29e-14 70.9
MS.gene32841.t1 AT1G26960 44.318 88 49 0 133 220 71 158 1.36e-14 72.4
MS.gene32841.t1 AT1G27045 51.389 72 35 0 133 204 68 139 1.99e-14 72.0
MS.gene32841.t1 AT4G40060 40.187 107 56 2 131 230 57 162 2.11e-14 72.4
MS.gene32841.t1 AT5G65310 42.857 98 49 1 130 220 51 148 3.20e-14 72.0
MS.gene32841.t1 AT5G65310 42.857 98 49 1 130 220 69 166 3.61e-14 72.0
MS.gene32841.t1 AT3G01220 35.593 118 66 2 99 211 53 165 4.80e-14 71.2
MS.gene32841.t1 AT2G22430 42.268 97 49 1 131 220 60 156 4.82e-14 71.6
MS.gene32841.t1 AT4G36740 40.816 98 58 0 129 226 51 148 8.88e-14 69.3
MS.gene32841.t1 AT4G36740 40.816 98 58 0 129 226 52 149 9.21e-14 69.3
MS.gene32841.t1 AT5G03790 36.066 122 73 1 127 243 71 192 1.28e-13 68.6
MS.gene32841.t1 AT2G46680 37.895 95 55 1 136 226 35 129 7.76e-12 64.7
MS.gene32841.t1 AT3G61890 37.000 100 60 1 136 235 33 129 7.91e-12 64.3
MS.gene32841.t1 AT2G46680 40.000 85 51 0 136 220 35 119 1.61e-11 63.5

Find 72 sgRNAs with CRISPR-Local

Find 107 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCTCTTCAATATTATTATT+AGG 0.146207 3.1:-82085390 None:intergenic
TCTCTTCAATATTATTATTA+GGG 0.203525 3.1:-82085389 None:intergenic
TATTTGCATAGAAGTGAATT+TGG 0.206531 3.1:-82086866 None:intergenic
ACTCCTAAGGTAAGAGAATA+TGG 0.209934 3.1:-82085360 None:intergenic
CTGGCTCATGATGTACTTCT+TGG 0.248157 3.1:-82085566 None:intergenic
CAAGATTGAGGATTGTTCTT+TGG 0.279678 3.1:-82085655 None:intergenic
ATTTCTCTTGGTCTTGGTCT+TGG 0.292030 3.1:+82085264 MS.gene32841:CDS
CTCCCTCTTGATGCTATTAG+AGG 0.294156 3.1:-82085526 None:intergenic
TAGAAGTGAATTTGGGCCTT+TGG 0.313028 3.1:-82086858 None:intergenic
AAGCTGCATGCAATTATTTA+CGG 0.314097 3.1:-82086730 None:intergenic
TTTGATATTTCTCTTGGTCT+TGG 0.326125 3.1:+82085258 MS.gene32841:CDS
AAATAATTGCATGCAGCTTC+CGG 0.343871 3.1:+82086734 MS.gene32841:CDS
TAAACAAAGCAAGAAGATTA+AGG 0.355539 3.1:+82085305 MS.gene32841:CDS
AGCAATAGTTGCAGAAGATT+TGG 0.363873 3.1:-82086907 None:intergenic
AGAAGTGAATTTGGGCCTTT+GGG 0.375155 3.1:-82086857 None:intergenic
AGAGGAGGATCCATGTAATA+AGG 0.377051 3.1:+82085332 MS.gene32841:CDS
AGCTGCATGCAATTATTTAC+GGG 0.380708 3.1:-82086729 None:intergenic
AAGCCTAAGTTTCTTCCTAC+TGG 0.393408 3.1:-82085631 None:intergenic
AAGAGAAATATCAAAATTCA+TGG 0.395654 3.1:-82085250 None:intergenic
ATTTGCATAGAAGTGAATTT+GGG 0.397943 3.1:-82086865 None:intergenic
CATCACGCTAGTAGAATTAG+AGG 0.402844 3.1:-82085487 None:intergenic
CTCATCGCCGGTGCCGGTGC+CGG 0.407320 3.1:-82086799 None:intergenic
GAGAGTAGAATGATCTTTGA+AGG 0.413896 3.1:-82085685 None:intergenic
TGGAGGTGTGGTTTCAAAAC+AGG 0.418208 3.1:+82086198 MS.gene32841:CDS
CAAAGAACAATCCTCAATCT+TGG 0.418520 3.1:+82085656 MS.gene32841:CDS
TCAACAAATGAAATCTTCTC+TGG 0.427530 3.1:-82085585 None:intergenic
GTTTATGTCTTTGTTCATGA+AGG 0.439832 3.1:-82085287 None:intergenic
TCAGAAGCAAAAGCAAGAAT+TGG 0.442692 3.1:+82086145 MS.gene32841:intron
CAAGAAGATTAAGGAGGAAG+AGG 0.445576 3.1:+82085314 MS.gene32841:CDS
CTTGATGCTATTAGAGGAGT+TGG 0.458659 3.1:-82085520 None:intergenic
GCCATTCTCATCGCCGGTGC+CGG 0.463859 3.1:-82086805 None:intergenic
TATTCTCTTACCTTAGGAGT+TGG 0.474125 3.1:+82085363 MS.gene32841:CDS
TGGCAAGCAAGCTGAATCTT+CGG 0.477653 3.1:+82086165 MS.gene32841:CDS
TACTACAACAAAGACTGAAT+CGG 0.478308 3.1:+82085434 MS.gene32841:CDS
TACCCATATTCTCTTACCTT+AGG 0.484324 3.1:+82085357 MS.gene32841:CDS
ATTTGCACCGGCACCGGCAC+CGG 0.484691 3.1:+82086792 MS.gene32841:CDS
GAAGGTGTCCTCCAAGATTG+AGG 0.485914 3.1:-82085667 None:intergenic
AACAGAAGAGAACAAGAGGC+TGG 0.492600 3.1:+82086662 MS.gene32841:CDS
ACCGGCACCGGCGATGAGAA+TGG 0.503337 3.1:+82086804 MS.gene32841:CDS
TATGGGTATGCCTTATTACA+TGG 0.508332 3.1:-82085342 None:intergenic
CTCCTAAGGTAAGAGAATAT+GGG 0.512060 3.1:-82085359 None:intergenic
AAAAGAATTTGCACCGGCAC+CGG 0.514546 3.1:+82086786 MS.gene32841:CDS
AGAGAACAAGAGGCTGGAAG+AGG 0.519798 3.1:+82086668 MS.gene32841:CDS
AATCAAAGCCGTGGTGTGAG+AGG 0.529506 3.1:-82086829 None:intergenic
AAAGCTGAAACAAACGGAAG+TGG 0.530773 3.1:+82086605 MS.gene32841:CDS
GGCTTTGATTCTCTGCCCAA+AGG 0.536458 3.1:+82086842 MS.gene32841:CDS
CTCTTCAATATTATTATTAG+GGG 0.543753 3.1:-82085388 None:intergenic
CTCTAACAGAAGAGAACAAG+AGG 0.544383 3.1:+82086658 MS.gene32841:CDS
CAGGACAAAGCTGAAACAAA+CGG 0.546061 3.1:+82086599 MS.gene32841:intron
AGATCATTCTACTCTCAATC+CGG 0.548356 3.1:+82085692 MS.gene32841:CDS
AGAAGGGCAAACTGTAAGAC+TGG 0.550866 3.1:-82086760 None:intergenic
ACTCCAGTAGGAAGAAACTT+AGG 0.556676 3.1:+82085628 MS.gene32841:CDS
GGACGAGACAAGTGGAGGTG+TGG 0.566994 3.1:+82086186 MS.gene32841:CDS
CAAACTGTAAGACTGGCAAC+CGG 0.573412 3.1:-82086753 None:intergenic
ATTAGGGGATCCAACTCCTA+AGG 0.574104 3.1:-82085373 None:intergenic
GAAGATTAAGGAGGAAGAGG+AGG 0.576607 3.1:+82085317 MS.gene32841:CDS
AGAGGAGCCATTCTCATCGC+CGG 0.585404 3.1:-82086811 None:intergenic
GGTTTCAAAACAGGAGGGCA+AGG 0.585888 3.1:+82086207 MS.gene32841:CDS
AGAACAATCCTCAATCTTGG+AGG 0.594021 3.1:+82085659 MS.gene32841:CDS
AGGTGTGGTTTCAAAACAGG+AGG 0.617414 3.1:+82086201 MS.gene32841:CDS
ACTCCTCTAATAGCATCAAG+AGG 0.625878 3.1:+82085523 MS.gene32841:CDS
ATGTAAATGATAACTCCAGT+AGG 0.626226 3.1:+82085616 MS.gene32841:CDS
TCTTGTAAAAGAATTTGCAC+CGG 0.632422 3.1:+82086780 MS.gene32841:CDS
GAATCTTCGGACGAGACAAG+TGG 0.639016 3.1:+82086178 MS.gene32841:CDS
CTCCTCTAATAGCATCAAGA+GGG 0.641427 3.1:+82085524 MS.gene32841:CDS
ACAAAGCAAGAAGATTAAGG+AGG 0.671987 3.1:+82085308 MS.gene32841:CDS
TGGGCAGAGAATCAAAGCCG+TGG 0.678880 3.1:-82086838 None:intergenic
TCTTCGGACGAGACAAGTGG+AGG 0.693782 3.1:+82086181 MS.gene32841:CDS
ACAAACGGAAGTGGACTGTG+AGG 0.696543 3.1:+82086614 MS.gene32841:CDS
GGTGTGGTTTCAAAACAGGA+GGG 0.700214 3.1:+82086202 MS.gene32841:CDS
GCTTAAGTCCATGAAAACAA+CGG 0.721053 3.1:+82086701 MS.gene32841:CDS
GAATGGCTCCTCTCACACCA+CGG 0.757581 3.1:+82086821 MS.gene32841:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAATTAATAAAATAAA+TGG - chr3.1:82085929-82085948 None:intergenic 0.0%
!! AAAAAATTAATAAAATAAAT+GGG - chr3.1:82085928-82085947 None:intergenic 0.0%
!!! TATTATTATAAATATTTTTA+TGG + chr3.1:82086280-82086299 MS.gene32841:intron 0.0%
!! TAATATTATGTAAAAAAACA+TGG - chr3.1:82086265-82086284 None:intergenic 10.0%
!!! AAATTGAATAAATTTTTTGA+TGG + chr3.1:82086006-82086025 MS.gene32841:intron 10.0%
!!! ATTATAAATATTTTTATGGT+TGG + chr3.1:82086284-82086303 MS.gene32841:intron 10.0%
!! ATAACATCATATTATATAGT+AGG - chr3.1:82086077-82086096 None:intergenic 15.0%
!! ATTTATTTGAGCAAATTAAT+TGG + chr3.1:82085768-82085787 MS.gene32841:intron 15.0%
!! TAACATCATATTATATAGTA+GGG - chr3.1:82086076-82086095 None:intergenic 15.0%
!! TCTCTTCAATATTATTATTA+GGG - chr3.1:82085392-82085411 None:intergenic 15.0%
!! TTAATAAAAATTAGTGCATT+TGG + chr3.1:82085949-82085968 MS.gene32841:intron 15.0%
!!! ATTTCAACTCAAAATTATTT+TGG + chr3.1:82086428-82086447 MS.gene32841:intron 15.0%
!! AAGAATTTGAAGATATATAC+CGG - chr3.1:82085714-82085733 None:intergenic 20.0%
!! AAGAGAAATATCAAAATTCA+TGG - chr3.1:82085253-82085272 None:intergenic 20.0%
!! ATTAACTAATATGTAGTACT+TGG + chr3.1:82085740-82085759 MS.gene32841:intron 20.0%
!! ATTTACCATATAATATATGC+AGG + chr3.1:82086580-82086599 MS.gene32841:intron 20.0%
!! CTCTTCAATATTATTATTAG+GGG - chr3.1:82085391-82085410 None:intergenic 20.0%
!! GAGATATCATAATCATTAAT+CGG - chr3.1:82086470-82086489 None:intergenic 20.0%
!! GTCTCTTCAATATTATTATT+AGG - chr3.1:82085393-82085412 None:intergenic 20.0%
!! GTTATTTATAAGTCATGAAT+TGG + chr3.1:82086092-82086111 MS.gene32841:intron 20.0%
!! TTTAATTACTTTGCTTTACA+AGG - chr3.1:82085989-82086008 None:intergenic 20.0%
!!! ATGAATTTTGATATTTCTCT+TGG + chr3.1:82085252-82085271 MS.gene32841:CDS 20.0%
!!! ATTAATCTTTTGAATCTTGT+GGG - chr3.1:82086374-82086393 None:intergenic 20.0%
! ATTTGCATAGAAGTGAATTT+GGG - chr3.1:82086868-82086887 None:intergenic 25.0%
! TAAACAAAGCAAGAAGATTA+AGG + chr3.1:82085305-82085324 MS.gene32841:CDS 25.0%
! TATTTGCATAGAAGTGAATT+TGG - chr3.1:82086869-82086888 None:intergenic 25.0%
! TTTGTCCTGCATATATTATA+TGG - chr3.1:82086588-82086607 None:intergenic 25.0%
!! AACTATCCTTAAGAGTTTTA+AGG - chr3.1:82086335-82086354 None:intergenic 25.0%
!! GATTAATCTTTTGAATCTTG+TGG - chr3.1:82086375-82086394 None:intergenic 25.0%
!! TGCAAATTCTTTTACAAGAA+GGG - chr3.1:82086779-82086798 None:intergenic 25.0%
!!! ATATTTTTATGGTTGGAATC+CGG + chr3.1:82086291-82086310 MS.gene32841:intron 25.0%
AAGCTGCATGCAATTATTTA+CGG - chr3.1:82086733-82086752 None:intergenic 30.0%
ATGTAAATGATAACTCCAGT+AGG + chr3.1:82085616-82085635 MS.gene32841:CDS 30.0%
CTTTACAAGGACTAAATACA+TGG - chr3.1:82085976-82085995 None:intergenic 30.0%
GTTTATGTCTTTGTTCATGA+AGG - chr3.1:82085290-82085309 None:intergenic 30.0%
TACTACAACAAAGACTGAAT+CGG + chr3.1:82085434-82085453 MS.gene32841:CDS 30.0%
TCAACAAATGAAATCTTCTC+TGG - chr3.1:82085588-82085607 None:intergenic 30.0%
TCTTGTAAAAGAATTTGCAC+CGG + chr3.1:82086780-82086799 MS.gene32841:CDS 30.0%
TTTGATATTTCTCTTGGTCT+TGG + chr3.1:82085258-82085277 MS.gene32841:CDS 30.0%
! GTGCAAATTCTTTTACAAGA+AGG - chr3.1:82086780-82086799 None:intergenic 30.0%
AAATAATTGCATGCAGCTTC+CGG + chr3.1:82086734-82086753 MS.gene32841:CDS 35.0%
AAGCGACCTTAAAACTCTTA+AGG + chr3.1:82086326-82086345 MS.gene32841:intron 35.0%
ACAAAGCAAGAAGATTAAGG+AGG + chr3.1:82085308-82085327 MS.gene32841:CDS 35.0%
ACTCCTAAGGTAAGAGAATA+TGG - chr3.1:82085363-82085382 None:intergenic 35.0%
AGATCATTCTACTCTCAATC+CGG + chr3.1:82085692-82085711 MS.gene32841:CDS 35.0%
AGCAATAGTTGCAGAAGATT+TGG - chr3.1:82086910-82086929 None:intergenic 35.0%
AGCTGCATGCAATTATTTAC+GGG - chr3.1:82086732-82086751 None:intergenic 35.0%
CAAAGAACAATCCTCAATCT+TGG + chr3.1:82085656-82085675 MS.gene32841:CDS 35.0%
CAAGATTGAGGATTGTTCTT+TGG - chr3.1:82085658-82085677 None:intergenic 35.0%
CTCCTAAGGTAAGAGAATAT+GGG - chr3.1:82085362-82085381 None:intergenic 35.0%
GAGAGTAGAATGATCTTTGA+AGG - chr3.1:82085688-82085707 None:intergenic 35.0%
GCTTAAGTCCATGAAAACAA+CGG + chr3.1:82086701-82086720 MS.gene32841:CDS 35.0%
TACCCATATTCTCTTACCTT+AGG + chr3.1:82085357-82085376 MS.gene32841:CDS 35.0%
TATTCTCTTACCTTAGGAGT+TGG + chr3.1:82085363-82085382 MS.gene32841:CDS 35.0%
TCAGAAGCAAAAGCAAGAAT+TGG + chr3.1:82086145-82086164 MS.gene32841:intron 35.0%
! TATGGGTATGCCTTATTACA+TGG - chr3.1:82085345-82085364 None:intergenic 35.0%
AAAGCTGAAACAAACGGAAG+TGG + chr3.1:82086605-82086624 MS.gene32841:CDS 40.0%
AAGCCTAAGTTTCTTCCTAC+TGG - chr3.1:82085634-82085653 None:intergenic 40.0%
ACTCCAGTAGGAAGAAACTT+AGG + chr3.1:82085628-82085647 MS.gene32841:CDS 40.0%
ACTCCTCTAATAGCATCAAG+AGG + chr3.1:82085523-82085542 MS.gene32841:CDS 40.0%
AGAACAATCCTCAATCTTGG+AGG + chr3.1:82085659-82085678 MS.gene32841:CDS 40.0%
AGAAGTGAATTTGGGCCTTT+GGG - chr3.1:82086860-82086879 None:intergenic 40.0%
AGAGGAGGATCCATGTAATA+AGG + chr3.1:82085332-82085351 MS.gene32841:CDS 40.0%
CAAGAAGATTAAGGAGGAAG+AGG + chr3.1:82085314-82085333 MS.gene32841:CDS 40.0%
CAGGACAAAGCTGAAACAAA+CGG + chr3.1:82086599-82086618 MS.gene32841:intron 40.0%
CATCACGCTAGTAGAATTAG+AGG - chr3.1:82085490-82085509 None:intergenic 40.0%
CTCCTCTAATAGCATCAAGA+GGG + chr3.1:82085524-82085543 MS.gene32841:CDS 40.0%
CTCTAACAGAAGAGAACAAG+AGG + chr3.1:82086658-82086677 MS.gene32841:CDS 40.0%
CTTGATGCTATTAGAGGAGT+TGG - chr3.1:82085523-82085542 None:intergenic 40.0%
TAGAAGTGAATTTGGGCCTT+TGG - chr3.1:82086861-82086880 None:intergenic 40.0%
TGCTTCTGATCATGTGTCAA+AGG - chr3.1:82086134-82086153 None:intergenic 40.0%
!! ATTTCTCTTGGTCTTGGTCT+TGG + chr3.1:82085264-82085283 MS.gene32841:CDS 40.0%
AACAGAAGAGAACAAGAGGC+TGG + chr3.1:82086662-82086681 MS.gene32841:CDS 45.0%
AGAAGGGCAAACTGTAAGAC+TGG - chr3.1:82086763-82086782 None:intergenic 45.0%
AGGTGTGGTTTCAAAACAGG+AGG + chr3.1:82086201-82086220 MS.gene32841:CDS 45.0%
ATTAGGGGATCCAACTCCTA+AGG - chr3.1:82085376-82085395 None:intergenic 45.0%
CAAACTGTAAGACTGGCAAC+CGG - chr3.1:82086756-82086775 None:intergenic 45.0%
CTCCCTCTTGATGCTATTAG+AGG - chr3.1:82085529-82085548 None:intergenic 45.0%
CTGGCTCATGATGTACTTCT+TGG - chr3.1:82085569-82085588 None:intergenic 45.0%
GAAGATTAAGGAGGAAGAGG+AGG + chr3.1:82085317-82085336 MS.gene32841:CDS 45.0%
GGTGTGGTTTCAAAACAGGA+GGG + chr3.1:82086202-82086221 MS.gene32841:CDS 45.0%
TGGCAAGCAAGCTGAATCTT+CGG + chr3.1:82086165-82086184 MS.gene32841:CDS 45.0%
! AAAAGAATTTGCACCGGCAC+CGG + chr3.1:82086786-82086805 MS.gene32841:CDS 45.0%
! TGGAGGTGTGGTTTCAAAAC+AGG + chr3.1:82086198-82086217 MS.gene32841:CDS 45.0%
!!! AAATATATTAGTTTTTTTTT+AGG + chr3.1:82085828-82085847 MS.gene32841:intron 5.0%
!!! AATTGAATAAATTTTTTAAA+TGG - chr3.1:82085902-82085921 None:intergenic 5.0%
AATCAAAGCCGTGGTGTGAG+AGG - chr3.1:82086832-82086851 None:intergenic 50.0%
AGAGAACAAGAGGCTGGAAG+AGG + chr3.1:82086668-82086687 MS.gene32841:CDS 50.0%
GAATCTTCGGACGAGACAAG+TGG + chr3.1:82086178-82086197 MS.gene32841:CDS 50.0%
GGTTTCAAAACAGGAGGGCA+AGG + chr3.1:82086207-82086226 MS.gene32841:CDS 50.0%
TTGAGCGTGCGAAGGATACT+TGG + chr3.1:82086402-82086421 MS.gene32841:intron 50.0%
! GGCTTTGATTCTCTGCCCAA+AGG + chr3.1:82086842-82086861 MS.gene32841:CDS 50.0%
! GTCGCTTGCATGTCTAAGAC+CGG - chr3.1:82086313-82086332 None:intergenic 50.0%
!! ACAAACGGAAGTGGACTGTG+AGG + chr3.1:82086614-82086633 MS.gene32841:CDS 50.0%
!! GAAGGTGTCCTCCAAGATTG+AGG - chr3.1:82085670-82085689 None:intergenic 50.0%
!! GGGTTGTGCCGTTGTTTTCA+TGG - chr3.1:82086712-82086731 None:intergenic 50.0%
AGAGGAGCCATTCTCATCGC+CGG - chr3.1:82086814-82086833 None:intergenic 55.0%
ATCCTTCGCACGCTCAACTG+CGG - chr3.1:82086399-82086418 None:intergenic 55.0%
GAATGGCTCCTCTCACACCA+CGG + chr3.1:82086821-82086840 MS.gene32841:CDS 55.0%
TCTTCGGACGAGACAAGTGG+AGG + chr3.1:82086181-82086200 MS.gene32841:CDS 55.0%
TGGGCAGAGAATCAAAGCCG+TGG - chr3.1:82086841-82086860 None:intergenic 55.0%
GGACGAGACAAGTGGAGGTG+TGG + chr3.1:82086186-82086205 MS.gene32841:CDS 60.0%
CTCCGCAGTTGAGCGTGCGA+AGG + chr3.1:82086394-82086413 MS.gene32841:intron 65.0%
GCCATTCTCATCGCCGGTGC+CGG - chr3.1:82086808-82086827 None:intergenic 65.0%
!! ACCGGCACCGGCGATGAGAA+TGG + chr3.1:82086804-82086823 MS.gene32841:CDS 65.0%
!! ATTTGCACCGGCACCGGCAC+CGG + chr3.1:82086792-82086811 MS.gene32841:CDS 65.0%
!! CTCATCGCCGGTGCCGGTGC+CGG - chr3.1:82086802-82086821 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 82085243 82086935 82085243 ID=MS.gene32841
chr3.1 mRNA 82085243 82086935 82085243 ID=MS.gene32841.t1;Parent=MS.gene32841
chr3.1 exon 82085243 82085713 82085243 ID=MS.gene32841.t1.exon1;Parent=MS.gene32841.t1
chr3.1 CDS 82085243 82085713 82085243 ID=cds.MS.gene32841.t1;Parent=MS.gene32841.t1
chr3.1 exon 82086149 82086228 82086149 ID=MS.gene32841.t1.exon2;Parent=MS.gene32841.t1
chr3.1 CDS 82086149 82086228 82086149 ID=cds.MS.gene32841.t1;Parent=MS.gene32841.t1
chr3.1 exon 82086602 82086935 82086602 ID=MS.gene32841.t1.exon3;Parent=MS.gene32841.t1
chr3.1 CDS 82086602 82086935 82086602 ID=cds.MS.gene32841.t1;Parent=MS.gene32841.t1
Gene Sequence

>MS.gene32841

ATGAACACCATGAATTTTGATATTTCTCTTGGTCTTGGTCTTGGCCTTCATGAACAAAGACATAAACAAAGCAAGAAGATTAAGGAGGAAGAGGAGGATCCATGTAATAAGGCATACCCATATTCTCTTACCTTAGGAGTTGGATCCCCTAATAATAATATTGAAGAGACAGATAATCATCAAGTAGCTACTACTACAACAAAGACTGAATCGGATGAGTTTATTTATAATTTGCATCAACGTGCCTCTAATTCTACTAGCGTGATGTCTTCATTTTCCAACTCCTCTAATAGCATCAAGAGGGAGAGAAATGATTTCAACAGCCAAGAAGTACATCATGAGCCAGAGAAGATTTCATTTGTTGATGCTGATGATGTAAATGATAACTCCAGTAGGAAGAAACTTAGGCTTACCAAAGAACAATCCTCAATCTTGGAGGACACCTTCAAAGATCATTCTACTCTCAATCCGGTATATATCTTCAAATTCTTCATTAAATTAACTAATATGTAGTACTTGGTTAGAATTTATTTGAGCAAATTAATTGGTTATAATTAGTGAGCATAATTAAATATGTTTTTTTACAAATATATTAGTTTTTTTTTAGGAATTAAATTTTTTTAATCTCTATAAAACGAGTATTATTTTAATTTAGTCCATTTAAAAAATTTATTCAATTTTACCCATTTATTTTATTAATTTTTTTTTAATAAAAATTAGTGCATTTGGTCCATGTATTTAGTCCTTGTAAAGCAAAGTAATTAAATTGAATAAATTTTTTGATGGACTAAATTCAAGTAGTAACTAGTAGAGAGAAAAAACATAATTAACCCTACTATATAATATGATGTTATTTATAAGTCATGAATTGGTTTGATTTCTCATTTTCCTTTGACACATGATCAGAAGCAAAAGCAAGAATTGGCAAGCAAGCTGAATCTTCGGACGAGACAAGTGGAGGTGTGGTTTCAAAACAGGAGGGCAAGGTTGTCATTCTATATTTACAATCATGATTCTCTCCATGTTTTTTTACATAATATTATTATAAATATTTTTATGGTTGGAATCCGGTCTTAGACATGCAAGCGACCTTAAAACTCTTAAGGATAGTTTGTCGTCTATTTAAGTCCCACAAGATTCAAAAGATTAATCTCCGCAGTTGAGCGTGCGAAGGATACTTGGTTAATTTCAACTCAAAATTATTTTGGATATAATAAATTGTCACCGATTAATGATTATGATATCTCATATGTTATCAAACAAATGAAATTATATATTTTCACATAGTAGAATGCATGTGTAAAATTTACACACAAGCAAAATTCTTAAATGTAATCATTTACCATATAATATATGCAGGACAAAGCTGAAACAAACGGAAGTGGACTGTGAGGCACTGAAGCATTGTTATGAAACTCTAACAGAAGAGAACAAGAGGCTGGAAGAGGAGTTGAAAGAGCTTAAGTCCATGAAAACAACGGCACAACCCGTAAATAATTGCATGCAGCTTCCGGTTGCCAGTCTTACAGTTTGCCCTTCTTGTAAAAGAATTTGCACCGGCACCGGCACCGGCGATGAGAATGGCTCCTCTCACACCACGGCTTTGATTCTCTGCCCAAAGGCCCAAATTCACTTCTATGCAAATAATAATAACTATACGTTCGCCAAATCTTCTGCAACTATTGCTAGCTAG
Protein sequence

>MS.gene32841.t1

MNTMNFDISLGLGLGLHEQRHKQSKKIKEEEEDPCNKAYPYSLTLGVGSPNNNIEETDNHQVATTTTKTESDEFIYNLHQRASNSTSVMSSFSNSSNSIKRERNDFNSQEVHHEPEKISFVDADDVNDNSSRKKLRLTKEQSSILEDTFKDHSTLNPKQKQELASKLNLRTRQVEVWFQNRRARTKLKQTEVDCEALKHCYETLTEENKRLEEELKELKSMKTTAQPVNNCMQLPVASLTVCPSCKRICTGTGTGDENGSSHTTALILCPKAQIHFYANNNNYTFAKSSATIAS