AlfalfaGEDB | MS.gene32951

M. sativa cultivar XinJiangDaYe / MS.gene32951

KEGG: K20558
GO: GO:0003674 GO:0005488 GO:0005515 GO:0046983
PFAM: PF00010.29

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene32951.t1	XP_013466371.1	84.5	277	18	2	1	257	1	272	5.20E-114	420.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene32951.t1	Q700C7	34.5	330	125	6	19	257	14	343	1.0e-30	135.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene32951.t1	A0A072VF69	84.5	277	18	2	1	257	1	272	3.7e-114	420.6

TFs/TRs:

Gene ID	Type	Classification
MS.gene32951.t1	TF	bHLH

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene32951.t1	MTR_1g028470	84.477	277	18	2	1	257	1	272	1.84e-155	434
MS.gene32951.t1	MTR_3g090850	52.597	308	77	14	15	257	5	308	3.05e-79	242
MS.gene32951.t1	MTR_2g022280	43.011	186	65	3	92	257	1	165	1.30e-39	136
MS.gene32951.t1	MTR_0194s0050	34.746	236	111	5	43	257	145	358	7.68e-34	126
MS.gene32951.t1	MTR_7g113830	36.634	202	106	5	66	257	93	282	7.07e-28	108
MS.gene32951.t1	MTR_1g082480	35.567	194	76	5	91	257	135	306	7.22e-26	103
MS.gene32951.t1	MTR_3g099620	32.301	226	115	4	54	257	79	288	7.45e-26	103
MS.gene32951.t1	MTR_1g082480	35.567	194	76	5	91	257	135	306	1.25e-25	103
MS.gene32951.t1	MTR_8g066770	36.932	176	93	5	91	257	113	279	4.64e-25	101
MS.gene32951.t1	MTR_5g064950	31.274	259	137	9	27	257	23	268	4.26e-23	95.9
MS.gene32951.t1	MTR_5g064950	31.621	253	132	9	27	251	23	262	5.28e-23	95.9
MS.gene32951.t1	MTR_8g024210	27.778	252	119	5	40	257	115	337	1.09e-16	79.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene32951.t1	AT5G53210	39.697	330	108	8	19	257	14	343	7.11e-55	180
MS.gene32951.t1	AT5G53210	39.697	330	108	8	19	257	14	343	1.11e-54	180
MS.gene32951.t1	AT3G06120	39.429	175	94	3	92	257	1	172	5.19e-35	124
MS.gene32951.t1	AT3G24140	36.364	209	101	3	69	257	177	373	1.95e-32	123
MS.gene32951.t1	AT3G24140	36.364	209	101	3	69	257	177	373	2.16e-32	122
MS.gene32951.t1	AT1G72210	34.043	188	81	6	91	257	124	289	7.44e-23	95.5
MS.gene32951.t1	AT1G72210	34.043	188	81	6	91	257	124	289	7.94e-23	95.5
MS.gene32951.t1	AT1G22490	35.838	173	95	4	91	257	114	276	5.17e-22	93.2
MS.gene32951.t1	AT1G22490	35.838	173	95	4	91	257	114	276	1.35e-21	92.4
MS.gene32951.t1	AT4G01460	34.091	176	99	4	90	257	113	279	1.50e-21	92.0
MS.gene32951.t1	AT4G01460	34.091	176	99	4	90	257	113	279	1.50e-21	92.0
MS.gene32951.t1	AT5G46690	56.164	73	30	1	91	161	87	159	9.16e-19	84.0
MS.gene32951.t1	AT5G46690	56.164	73	30	1	91	161	87	159	1.81e-18	83.6
MS.gene32951.t1	AT3G61950	54.930	71	30	1	91	159	126	196	5.24e-18	82.0
MS.gene32951.t1	AT3G61950	54.167	72	31	1	91	160	126	197	7.05e-18	81.6
MS.gene32951.t1	AT3G61950	54.930	71	30	1	91	159	177	247	1.16e-17	81.6
MS.gene32951.t1	AT3G61950	54.167	72	31	1	91	160	177	248	1.29e-17	81.6
MS.gene32951.t1	AT2G46810	58.065	62	26	0	91	152	120	181	1.37e-17	80.9
MS.gene32951.t1	AT5G65320	55.556	72	28	1	79	150	93	160	1.62e-17	80.1
MS.gene32951.t1	AT2G46810	58.065	62	26	0	91	152	120	181	1.95e-17	80.9
MS.gene32951.t1	AT5G65320	55.556	72	28	1	79	150	93	160	2.58e-17	80.1
MS.gene32951.t1	AT2G46810	58.065	62	26	0	91	152	180	241	3.58e-17	80.1
MS.gene32951.t1	AT2G46810	58.065	62	26	0	91	152	192	253	4.03e-17	80.1

Find 28 sgRNAs with CRISPR-Local

Find 58 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTCTTTGTTTCTTGGATTCT+AGG	0.149526	1.2:+14733592	None:intergenic
GCTATACTTGTTCACTATTA+AGG	0.194492	1.2:-14733219	None:intergenic
TATGGAGTTCTTGGACTAAT+TGG	0.233920	1.2:+14733510	None:intergenic
CTCATTGATGTTATCATTGA+TGG	0.240583	1.2:+14733422	None:intergenic
AGCCATTGATATGGAGTTCT+TGG	0.282669	1.2:+14733501	None:intergenic
CTAATTGGTAAGATTATTCT+AGG	0.295024	1.2:+14733525	None:intergenic
CATTGTTGTAGCCATTGATA+TGG	0.334687	1.2:+14733492	None:intergenic
ATATTCAATGAAACTGAGTT+TGG	0.348570	1.2:-14734267	MS.gene32951:CDS
AACAAGGTGTCATGATCTAT+TGG	0.372909	1.2:+14734300	None:intergenic
TGTGACTTCTTCAATTATAG+AGG	0.399402	1.2:-14734094	MS.gene32951:CDS
ATGGAAGAAGCTGAAGAAGT+TGG	0.426375	1.2:+14733441	None:intergenic
GGACTTAGTGGTAAAGGTCT+TGG	0.429539	1.2:+14733546	None:intergenic
AAGATTATTCTAGGACTTAG+TGG	0.440624	1.2:+14733534	None:intergenic
ATTCTAGGACTTAGTGGTAA+AGG	0.492814	1.2:+14733540	None:intergenic
TGTCGCGAATTCAACATCTT+CGG	0.496233	1.2:-14733397	MS.gene32951:CDS
GTACAAAATGGAAACTCAAA+AGG	0.509601	1.2:-14734325	None:intergenic
GTCCAAGAACTCCATATCAA+TGG	0.523072	1.2:-14733503	MS.gene32951:CDS
ATCTTCGGTTGCTGATGTTG+AGG	0.535328	1.2:-14733382	MS.gene32951:intron
TGAAACTGAGTTTGGTTCAG+AGG	0.550414	1.2:-14734259	MS.gene32951:CDS
TTGGAGATGGCTCAGATGAA+AGG	0.570619	1.2:+14733460	None:intergenic
ACATCGTATGTCGCATATCA+CGG	0.587471	1.2:-14734052	MS.gene32951:CDS
GATCAAGCATCAATAGTTGA+AGG	0.590664	1.2:-14733657	MS.gene32951:CDS
AATAGTGAACAAGTATAGCA+TGG	0.592880	1.2:+14733223	None:intergenic
GAAGCTGAAGAAGTTGGAGA+TGG	0.605113	1.2:+14733447	None:intergenic
ATAGAGGAACAAAACAACGA+TGG	0.690625	1.2:-14734078	MS.gene32951:CDS
CAATGGCTACAACAATGTGG+TGG	0.701552	1.2:-14733486	MS.gene32951:CDS
TATCAATGGCTACAACAATG+TGG	0.711948	1.2:-14733489	MS.gene32951:CDS
GAATATATCAGATAACAACA+AGG	0.741545	1.2:+14734284	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AGTTTTAAATGAAACTAGTT+AGG	-	chr1.2:14733645-14733664	MS.gene32951:CDS	20.0%
!!!	ATTATTGAAGTTTTCCAAAA+TGG	+	chr1.2:14733314-14733333	None:intergenic	20.0%
!!!	GTTTTAAATGAAACTAGTTA+GGG	-	chr1.2:14733646-14733665	MS.gene32951:CDS	20.0%
!!!	TTTTAAATGAAACTAGTTAG+GGG	-	chr1.2:14733647-14733666	MS.gene32951:CDS	20.0%
!	ATATTCAATGAAACTGAGTT+TGG	-	chr1.2:14733271-14733290	MS.gene32951:CDS	25.0%
!	ATTACTCTTCTATATGGTTT+AGG	-	chr1.2:14733856-14733875	MS.gene32951:intron	25.0%
!	CAACTAATTACTCTTCTATA+TGG	-	chr1.2:14733850-14733869	MS.gene32951:intron	25.0%
!	CTAATTGGTAAGATTATTCT+AGG	+	chr1.2:14734016-14734035	None:intergenic	25.0%
!	GAATATATCAGATAACAACA+AGG	+	chr1.2:14733257-14733276	None:intergenic	25.0%
!	TTACTCTTCTATATGGTTTA+GGG	-	chr1.2:14733857-14733876	MS.gene32951:intron	25.0%
!!!	AAGATGTTGTTTTTGAGTTT+TGG	+	chr1.2:14733357-14733376	None:intergenic	25.0%
!!!	GTAAACTTTTCTTTGTTTCT+TGG	+	chr1.2:14733957-14733976	None:intergenic	25.0%
	AATAGTGAACAAGTATAGCA+TGG	+	chr1.2:14734318-14734337	None:intergenic	30.0%
	CATAAACCAGACCAAATTTA+TGG	-	chr1.2:14733681-14733700	MS.gene32951:intron	30.0%
	CTCATTGATGTTATCATTGA+TGG	+	chr1.2:14734119-14734138	None:intergenic	30.0%
	TGTGACTTCTTCAATTATAG+AGG	-	chr1.2:14733444-14733463	MS.gene32951:CDS	30.0%
!	ACTCTTTTGACATAAAAGCA+AGG	+	chr1.2:14733571-14733590	None:intergenic	30.0%
!	AGAGGATCTATTTTCCATTT+TGG	-	chr1.2:14733297-14733316	MS.gene32951:CDS	30.0%
!	ATGTTGAGGTTAAGTTTTTG+TGG	-	chr1.2:14734170-14734189	MS.gene32951:CDS	30.0%
!	TTCTTTGTTTCTTGGATTCT+AGG	+	chr1.2:14733949-14733968	None:intergenic	30.0%
!!	AAGATTATTCTAGGACTTAG+TGG	+	chr1.2:14734007-14734026	None:intergenic	30.0%
!!	GAGTTTTGGTCATTGATAAT+GGG	+	chr1.2:14733343-14733362	None:intergenic	30.0%
!!	TGAGTTTTGGTCATTGATAA+TGG	+	chr1.2:14733344-14733363	None:intergenic	30.0%
	ATAGAGGAACAAAACAACGA+TGG	-	chr1.2:14733460-14733479	MS.gene32951:CDS	35.0%
	CATTGTTGTAGCCATTGATA+TGG	+	chr1.2:14734049-14734068	None:intergenic	35.0%
	GATCAAGCATCAATAGTTGA+AGG	-	chr1.2:14733881-14733900	MS.gene32951:intron	35.0%
	TATCAATGGCTACAACAATG+TGG	-	chr1.2:14734049-14734068	MS.gene32951:CDS	35.0%
	TATGGAGTTCTTGGACTAAT+TGG	+	chr1.2:14734031-14734050	None:intergenic	35.0%
!	AACAAGGTGTCATGATCTAT+TGG	+	chr1.2:14733241-14733260	None:intergenic	35.0%
!	TGCTCATTTTCAGAAACTTG+AGG	+	chr1.2:14733388-14733407	None:intergenic	35.0%
!!	ATTCTAGGACTTAGTGGTAA+AGG	+	chr1.2:14734001-14734020	None:intergenic	35.0%
	ACATCGTATGTCGCATATCA+CGG	-	chr1.2:14733486-14733505	MS.gene32951:CDS	40.0%
	ACCAGACCAAATTTATGGTC+TGG	-	chr1.2:14733686-14733705	MS.gene32951:intron	40.0%
	ACCAGACCATAAATTTGGTC+TGG	+	chr1.2:14733690-14733709	None:intergenic	40.0%
	ACTGGACCAGACCATAAATT+TGG	+	chr1.2:14733695-14733714	None:intergenic	40.0%
	AGCCATTGATATGGAGTTCT+TGG	+	chr1.2:14734040-14734059	None:intergenic	40.0%
	ATGGAAGAAGCTGAAGAAGT+TGG	+	chr1.2:14734100-14734119	None:intergenic	40.0%
	ATTGCATCAACCGTAGACTA+CGG	+	chr1.2:14733743-14733762	None:intergenic	40.0%
	GTCCAAGAACTCCATATCAA+TGG	-	chr1.2:14734035-14734054	MS.gene32951:CDS	40.0%
	TAACTACGAACCGTAGTCTA+CGG	-	chr1.2:14733730-14733749	MS.gene32951:intron	40.0%
	TAGACTACGGTTCGTAGTTA+GGG	+	chr1.2:14733730-14733749	None:intergenic	40.0%
	TGTCGCGAATTCAACATCTT+CGG	-	chr1.2:14734141-14734160	MS.gene32951:CDS	40.0%
!	TGAAACTGAGTTTGGTTCAG+AGG	-	chr1.2:14733279-14733298	MS.gene32951:CDS	40.0%
	AACCGTTTCGTCACGAATTC+CGG	-	chr1.2:14734211-14734230	MS.gene32951:CDS	45.0%
	AGACTACGGTTCGTAGTTAG+GGG	+	chr1.2:14733729-14733748	None:intergenic	45.0%
	ATCTTCGGTTGCTGATGTTG+AGG	-	chr1.2:14734156-14734175	MS.gene32951:CDS	45.0%
	CAATGGCTACAACAATGTGG+TGG	-	chr1.2:14734052-14734071	MS.gene32951:CDS	45.0%
	GAAGCTGAAGAAGTTGGAGA+TGG	+	chr1.2:14734094-14734113	None:intergenic	45.0%
	GGACTTAGTGGTAAAGGTCT+TGG	+	chr1.2:14733995-14734014	None:intergenic	45.0%
	GTAGACTACGGTTCGTAGTT+AGG	+	chr1.2:14733731-14733750	None:intergenic	45.0%
	TTGGAGATGGCTCAGATGAA+AGG	+	chr1.2:14734081-14734100	None:intergenic	45.0%
!	ATTATTCTAAGAGCCTGCCC+CGG	+	chr1.2:14734233-14734252	None:intergenic	45.0%
!	TTAGGGGTGTGCAAAAGAAC+TGG	+	chr1.2:14733713-14733732	None:intergenic	45.0%
!!!	TAATTTTAATCTTAATAAAA+AGG	+	chr1.2:14733787-14733806	None:intergenic	5.0%
	ACCGTTTCGTCACGAATTCC+GGG	-	chr1.2:14734212-14734231	MS.gene32951:CDS	50.0%
	CCCCGGAATTCGTGACGAAA+CGG	+	chr1.2:14734216-14734235	None:intergenic	55.0%
	CCGTTTCGTCACGAATTCCG+GGG	-	chr1.2:14734213-14734232	MS.gene32951:CDS	55.0%
	TTCGTCACGAATTCCGGGGC+AGG	-	chr1.2:14734217-14734236	MS.gene32951:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	14733220	14734340	14733220	ID=MS.gene32951
chr1.2	mRNA	14733220	14734340	14733220	ID=MS.gene32951.t1;Parent=MS.gene32951
chr1.2	exon	14733972	14734340	14733972	ID=MS.gene32951.t1.exon1;Parent=MS.gene32951.t1
chr1.2	CDS	14733972	14734340	14733972	ID=cds.MS.gene32951.t1;Parent=MS.gene32951.t1
chr1.2	exon	14733383	14733682	14733383	ID=MS.gene32951.t1.exon2;Parent=MS.gene32951.t1
chr1.2	CDS	14733383	14733682	14733383	ID=cds.MS.gene32951.t1;Parent=MS.gene32951.t1
chr1.2	exon	14733220	14733321	14733220	ID=MS.gene32951.t1.exon3;Parent=MS.gene32951.t1
chr1.2	CDS	14733220	14733321	14733220	ID=cds.MS.gene32951.t1;Parent=MS.gene32951.t1

Gene Sequence

>MS.gene32951

ATGGAAACTCAAAAGGCTCCAATAGATCATGACACCTTGTTGTTATCTGATATATTCAATGAAACTGAGTTTGGTTCAGAGGATCTATTTTCCATTTTGGAAAACTTCAATAATTTTTCTCCCATTATCAATGACCAAAACTCAAAAACAACATCTTCAAGTGCTCCTCAAGTTTCTGAAAATGAGCATGCTGAGACATCACAGAAATGCAAGAGACAGAAGATTGTGACTTCTTCAATTATAGAGGAACAAAACAACGATGGACAACATCGTATGTCGCATATCACGGTCGAACGCAATCGAAGAAAGCAAATGAATGAGCAGCTATCTATCTTGAAGTCACTTATGCCTTGCTTTTATGTCAAAAGAGTGAGTACATATTAACTAACTTGTCACAAATTCAAATTCTAAATAATTTTTTTCAAAGTTTTAAATGAAACTAGTTAGGGGTGTGCATGATTCATAAACCAGACCAAATTTATGGTCTGGTCCAGTTCTTTTGCACACCCCTAACTACGAACCGTAGTCTACGGTTGATGCAATAACAATTGCAATTGTTTGAAACCTTTTTATTAAGATTAAAATTATGTTTATCTTTCTTTAAAATTATTATTTTTTGCATAAAAAATGCAACTAATTACTCTTCTATATGGTTTAGGGAGATCAAGCATCAATAGTTGAAGGTGTAATTGATTACATCAACGAGTTACATCAACTACTACAATGCCTAGAATCCAAGAAACAAAGAAAAGTTTACAGTGAAGTAACAAGTCCAAGACCTTTACCACTAAGTCCTAGAATAATCTTACCAATTAGTCCAAGAACTCCATATCAATGGCTACAACAATGTGGTGGTTACCTTTCATCTGAGCCATCTCCAACTTCTTCAGCTTCTTCCATCAATGATAACATCAATGAGCTTGTCGCGAATTCAACATCTTCGGTTGCTGATGTTGAGGTTAAGTTTTTGTGGTTCACATGTGCTTTTGAAAACCGTTTCGTCACGAATTCCGGGGCAGGCTCTTAGAATAATGTCTGTTCTTGAAGATCTAATGCTTGAGATTGTTCATGTTAGAGTTAATACTGCTGATGAAACCATGCTATACTTGTTCACTATTAAG

Protein sequence

>MS.gene32951.t1

METQKAPIDHDTLLLSDIFNETEFGSEDLFSILENFNNFSPIINDQNSKTTSSSAPQVSENEHAETSQKCKRQKIVTSSIIEEQNNDGQHRMSHITVERNRRKQMNEQLSILKSLMPCFYVKRGDQASIVEGVIDYINELHQLLQCLESKKQRKVYSEVTSPRPLPLSPRIILPISPRTPYQWLQQCGGYLSSEPSPTSSASSINDNINELVANSTSSVADVEALRIMSVLEDLMLEIVHVRVNTADETMLYLFTIK