AlfalfaGEDB | MS.gene32959

M. sativa cultivar XinJiangDaYe / MS.gene32959

KEGG: K09422
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0097159 GO:1901363
PFAM: PF00249.34

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene32959.t1	XP_013465930.1	93.5	168	10	1	20	187	86	252	8.40E-82	313.2

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene32959.t1	F4JSU0	70.6	34	10	0	20	53	86	119	8.2e-09	62.0

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene32959.t1	A0A072VEE8	93.5	168	10	1	20	187	86	252	6.1e-82	313.2

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene32959.t1	TF	MYB-related

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene32959.t1	MTR_1g017000	93.452	168	10	1	20	187	86	252	6.34e-111	317
MS.gene32959.t1	MTR_6g009430	50.725	69	30	3	20	85	85	152	1.71e-12	65.1
MS.gene32959.t1	MTR_3g103570	41.096	73	43	0	20	92	86	158	1.99e-12	64.7
MS.gene32959.t1	MTR_7g102110	56.452	62	20	2	20	74	86	147	2.80e-12	63.9
MS.gene32959.t1	MTR_4g057635	70.588	34	10	0	20	53	86	119	2.04e-11	61.6
MS.gene32959.t1	MTR_6g090405	62.500	40	15	0	15	54	81	120	2.06e-11	61.2
MS.gene32959.t1	MTR_1g017140	67.647	34	11	0	20	53	86	119	5.14e-11	60.8
MS.gene32959.t1	MTR_5g014990	67.647	34	11	0	20	53	86	119	5.26e-11	60.5
MS.gene32959.t1	MTR_4g091490	53.333	45	19	1	20	64	86	128	5.85e-11	60.5

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene32959.t1	AT5G16770	38.136	118	60	6	20	134	85	192	1.34e-12	65.1
MS.gene32959.t1	AT5G16770	38.136	118	60	6	20	134	85	192	1.34e-12	65.1
MS.gene32959.t1	AT5G16770	38.136	118	60	6	20	134	85	192	2.20e-12	64.7
MS.gene32959.t1	AT5G16770	38.136	118	60	6	20	134	85	192	2.20e-12	64.7
MS.gene32959.t1	AT5G57620	53.846	52	21	1	20	71	86	134	1.65e-11	62.0
MS.gene32959.t1	AT4G37780	70.588	34	10	0	20	53	86	119	2.21e-11	61.6
MS.gene32959.t1	AT3G23250	45.000	80	41	2	15	92	23	101	3.65e-11	60.5
MS.gene32959.t1	AT5G23000	63.158	38	14	0	15	52	81	118	8.09e-11	60.1
MS.gene32959.t1	AT3G23250	45.000	80	41	2	15	92	80	158	9.21e-11	59.7
MS.gene32959.t1	AT5G65790	69.697	33	10	0	20	52	86	118	9.63e-11	60.1

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Find 30 sgRNAs with CRISPR-Local

Find 78 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACTGAAGAAGAAGATTT+TGG	0.243833	1.1:+5466551	MS.gene32959:CDS
ACATCATTGTCTGTTCTGCC+AGG	0.476737	1.1:-5466510	None:intergenic
ACTTTATTGTCTAGCATAAT+TGG	0.238985	1.1:-5466717	None:intergenic
AGAATAGAGGGTGAAAATGA+TGG	0.524462	1.1:-5465557	None:intergenic
AGCATAATTGGAAGTGGTGA+AGG	0.515734	1.1:-5466705	None:intergenic
ATAATTGGAAGTGGTGAAGG+TGG	0.597724	1.1:-5466702	None:intergenic
ATCTTAACTGCTTTCCTGAG+TGG	0.564962	1.1:+5466922	MS.gene32959:CDS
ATGGAATCATTGATGGACTT+TGG	0.403753	1.1:+5466867	MS.gene32959:CDS
ATTGCAATTCAAGATCAAGC+TGG	0.635582	1.1:+5466834	MS.gene32959:CDS
CAGCAATATTATTAATCCTT+TGG	0.356887	1.1:+5466785	MS.gene32959:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CAAGATTAAGACCCAAAAAA+TGG	+	chr1.1:5466226-5466245	MS.gene32959:intron	30.0%
GAAATTATAACAACCAACTG+GGG	+	chr1.1:5465873-5465892	MS.gene32959:intron	30.0%
GAGAAATTATAACAACCAAC+TGG	+	chr1.1:5465871-5465890	MS.gene32959:intron	30.0%
GTACTATGAAACTAACTTTG+AGG	-	chr1.1:5466405-5466424	None:intergenic	30.0%
TAATTTCTCACTTTGACTGA+AGG	-	chr1.1:5466026-5466045	None:intergenic	30.0%
TAGTTTCATAGTACTTCAAG+AGG	+	chr1.1:5466411-5466430	MS.gene32959:intron	30.0%
TGAATCTATGGAATCATTGA+TGG	+	chr1.1:5466860-5466879	MS.gene32959:CDS	30.0%
TGAGAACTATTCAAACTTTG+AGG	-	chr1.1:5466443-5466462	None:intergenic	30.0%
TTCCCATGATGTTATAAACT+AGG	+	chr1.1:5465920-5465939	MS.gene32959:intron	30.0%
AGAATAGAGGGTGAAAATGA+TGG	-	chr1.1:5465560-5465579	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1.1	gene	5465530	5466980	5465530	ID=MS.gene32959
chr1.1	mRNA	5465530	5466980	5465530	ID=MS.gene32959.t1;Parent=MS.gene32959
chr1.1	exon	5465530	5465597	5465530	ID=MS.gene32959.t1.exon1;Parent=MS.gene32959.t1
chr1.1	CDS	5465530	5465597	5465530	ID=cds.MS.gene32959.t1;Parent=MS.gene32959.t1
chr1.1	exon	5466485	5466980	5466485	ID=MS.gene32959.t1.exon2;Parent=MS.gene32959.t1
chr1.1	CDS	5466485	5466980	5466485	ID=cds.MS.gene32959.t1;Parent=MS.gene32959.t1

Gene Sequence

>MS.gene32959

ATGGAGGCTTCACTGAAGAAGAGGATGCCATCATTTTCACCCTCTATTCTCAAATGGGTAGCAGGCAAGTACTATTTCCAACTTATTTAATTAAGTTGTATCTATTTTCACTATATAATTTCCAAATTGTTATTATTATAAACGTTGATCATTGTTAACTGTTATAATCACTTTATTTAATTTAGTATTATATATAGTTTTTCATAAGAGTTGATCATTGTTAAGTGCTAACTATTACAACTTTATTTAATTTAGTACTATGTATTGTTTTTCTTTAAGAGTTGATGATTTTTAATGTGTACAAGTTGGAAAGATTTGTTTTTTCATATAGATAATAAATTGAGAAATTATAACAACCAACTGGGGTTGAGTCAACTTGAAGGGGATAACTTCCCATGATGTTATAAACTAGGAATTCCGAGACTAAAATTTAAATTTCAGTTGGGAGAGATGTCGTATTTAGAGTTTGATGGTGTTTTAAATAAAATTACAACCTTCAGTCAAAGTGAGAAATTATAACCGACAGTCCGACATAATGTTGGTCTAAGTGTAACTGGATTGGACTCAAATTTTTTTAAGCAATGTCTCATGTTCAAACTTTATCAACAGAAAAAATGTGATTAGAAGGAGAGAAACTACTAAATGTTGGATTTTATTTAATTATCTGTCGAACCTGTGATGATTATGGTCTTTCCCCAAGATTAAGACCCAAAAAATGGTTATAGAATATAGATGCTTGATACCAATAACATGATTTTAAAAAAAGTGAGAAATTATGGATTGATATTCTATCATTTAATTATGCATATATACAAAATAAATGTACTATCTATCTATTAGTAGTATTGATTTTTTATAGTCAGTTGAATGGTCCTCAAAGTTAGTTTCATAGTACTTCAAGAGGAATATGCCTCAAAGTTTGAATAGTTCTCATGAAATTTGTGATTTTGTGCAGATGGTCTGCCATAGCATCTAAACTACCTGGCAGAACAGACAATGATGTGAAAAACTATTGGAACACAAAACTGAAGAAGAAGATTTTGGCAGGAATAGTTAAGCTCAAACCATTGACTGAAAATGACAATACTATCCCTTCAACTCCATCACTAACTCAAAGTTCCAACCCTCAAAATTCAGAAATCAATTTCCCTGCTTCACAAAATCAACATTCTCCACCTTCACCACTTCCAATTATGCTAGACAATAAAGTTGACAAAATTCATGATGCTGTAGTATCAGAAATTGGTACAAGTAACAGCAATATTATTAATCCTTTGGTGTCAATTTCTCAAGACTCTTCAAGCATTGCAATTCAAGATCAAGCTGGTGATGAATCTATGGAATCATTGATGGACTTTGGCTTTGGATTTCCTCATGATGATCATGTCAATGATCTTAACTGCTTTCCTGAGTGGGTTGATTTTAGTTATGCTGACATTAAGCCAAATTGA

Protein sequence

>MS.gene32959.t1

MEASLKKRMPSFSPSILKWVAGKWSAIASKLPGRTDNDVKNYWNTKLKKKILAGIVKLKPLTENDNTIPSTPSLTQSSNPQNSEINFPASQNQHSPPSPLPIMLDNKVDKIHDAVVSEIGTSNSNIINPLVSISQDSSSIAIQDQAGDESMESLMDFGFGFPHDDHVNDLNCFPEWVDFSYADIKPN