AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene32967

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32967.t1 MTR_5g076070 48.485 264 134 1 38 301 19 280 2.75e-86 275
MS.gene32967.t1 MTR_1g070355 46.863 271 144 0 39 309 39 309 4.39e-83 276
MS.gene32967.t1 MTR_6g022080 47.000 200 106 0 39 238 50 249 1.53e-60 205
MS.gene32967.t1 MTR_6g022070 55.090 167 75 0 369 535 1 167 1.03e-58 203
MS.gene32967.t1 MTR_6g022070 30.769 130 82 4 615 741 167 291 7.79e-11 64.7
MS.gene32967.t1 MTR_8g066350 51.872 187 67 2 1 182 1 169 7.73e-53 181
MS.gene32967.t1 MTR_2g101460 49.219 128 65 0 55 182 36 163 2.64e-34 130
MS.gene32967.t1 MTR_2g101460 46.154 143 77 0 40 182 11 153 5.41e-34 129
MS.gene32967.t1 MTR_6g053140 43.949 157 76 3 25 180 58 203 4.47e-32 124
MS.gene32967.t1 MTR_6g051425 29.070 258 177 4 86 338 29 285 1.02e-31 127
MS.gene32967.t1 MTR_7g446480 37.500 176 108 1 151 324 140 315 1.19e-30 123
MS.gene32967.t1 MTR_7g446510 37.500 176 108 1 151 324 155 330 2.56e-30 122
MS.gene32967.t1 MTR_7g446520 30.000 280 172 5 33 309 49 307 1.16e-29 120
MS.gene32967.t1 MTR_3g448650 42.188 128 72 1 25 150 31 158 1.05e-26 107
MS.gene32967.t1 MTR_1g052260 43.511 131 65 3 35 156 28 158 4.46e-26 105
MS.gene32967.t1 MTR_8g045760 43.529 85 48 0 203 287 1 85 5.99e-22 93.6
MS.gene32967.t1 MTR_0060s0090 38.400 125 77 0 35 159 17 141 1.19e-21 92.8
MS.gene32967.t1 MTR_0268s0050 36.170 141 88 2 616 755 114 253 4.05e-21 94.4
MS.gene32967.t1 MTR_3g450850 28.512 242 159 8 86 315 59 298 1.71e-19 91.3
MS.gene32967.t1 MTR_4g035820 25.263 285 187 7 43 319 58 324 2.11e-19 93.6
MS.gene32967.t1 MTR_4g050510 36.090 133 71 2 60 192 64 182 2.80e-16 78.6
MS.gene32967.t1 MTR_6g463250 39.394 99 45 2 61 159 75 158 5.76e-16 77.0
MS.gene32967.t1 MTR_0604s0020 48.649 74 38 0 85 158 3 76 6.43e-16 73.6
MS.gene32967.t1 MTR_7g028855 27.749 191 135 2 25 215 74 261 1.86e-14 77.0
MS.gene32967.t1 MTR_2g069720 35.238 105 66 1 4 108 29 131 2.95e-14 71.6
MS.gene32967.t1 MTR_0645s0010 42.857 91 49 2 447 537 1 88 6.89e-14 69.3
MS.gene32967.t1 MTR_0558s0020 24.082 245 167 5 89 314 51 295 1.16e-13 72.8
MS.gene32967.t1 MTR_0402s0040 28.655 171 106 5 84 247 36 197 1.94e-12 70.9
MS.gene32967.t1 MTR_8g446020 25.140 179 130 2 138 314 79 255 3.43e-12 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32967.t1 AT4G38180 31.947 601 382 8 44 632 52 637 1.75e-98 323
MS.gene32967.t1 AT1G76320 29.711 589 378 14 86 663 9 572 2.14e-73 253
MS.gene32967.t1 AT3G22170 27.856 639 401 14 14 629 17 618 3.11e-73 256
MS.gene32967.t1 AT3G22170 27.856 639 401 14 14 629 17 618 3.11e-73 256
MS.gene32967.t1 AT1G76320 29.711 589 378 14 86 663 9 572 3.30e-73 254
MS.gene32967.t1 AT1G76320 29.711 589 378 14 86 663 9 572 3.30e-73 254
MS.gene32967.t1 AT1G76320 29.711 589 378 14 86 663 9 572 3.77e-73 253
MS.gene32967.t1 AT4G15090 27.679 560 384 9 85 632 62 612 1.53e-72 254
MS.gene32967.t1 AT4G15090 27.679 560 384 9 85 632 62 612 1.53e-72 254
MS.gene32967.t1 AT4G15090 27.679 560 384 9 85 632 62 612 1.53e-72 254
MS.gene32967.t1 AT4G15090 27.679 560 384 9 85 632 62 612 1.53e-72 254
MS.gene32967.t1 AT4G15090 27.679 560 384 9 85 632 62 612 1.53e-72 254
MS.gene32967.t1 AT2G27110 27.536 552 363 10 89 634 64 584 1.72e-69 246
MS.gene32967.t1 AT2G27110 27.536 552 363 10 89 634 64 584 1.72e-69 246
MS.gene32967.t1 AT2G27110 30.583 412 275 4 228 634 34 439 7.60e-67 236
MS.gene32967.t1 AT3G06250 27.057 547 346 10 89 629 205 704 1.92e-66 236
MS.gene32967.t1 AT3G06250 27.057 547 346 10 89 629 205 704 1.92e-66 236
MS.gene32967.t1 AT3G06250 27.057 547 346 10 89 629 205 704 1.92e-66 236
MS.gene32967.t1 AT5G18960 26.776 549 348 12 89 629 226 728 3.63e-62 224
MS.gene32967.t1 AT5G18960 26.776 549 348 12 89 629 226 728 3.63e-62 224
MS.gene32967.t1 AT4G38170 30.137 365 241 6 273 629 41 399 1.09e-58 209
MS.gene32967.t1 AT1G80010 29.174 569 364 14 86 629 107 661 8.18e-57 207
MS.gene32967.t1 AT4G19990 25.514 584 366 13 86 643 33 573 2.92e-54 201
MS.gene32967.t1 AT4G19990 25.514 584 366 13 86 643 33 573 3.25e-54 201
MS.gene32967.t1 AT4G19990 25.514 584 366 13 86 643 33 573 3.25e-54 201
MS.gene32967.t1 AT4G19990 25.514 584 366 13 86 643 47 587 3.88e-54 201
MS.gene32967.t1 AT4G19990 25.514 584 366 13 86 643 33 573 7.28e-54 200
MS.gene32967.t1 AT4G19990 25.514 584 366 13 86 643 33 573 7.28e-54 200
MS.gene32967.t1 AT2G32250 28.571 406 277 5 266 666 187 584 1.52e-53 199
MS.gene32967.t1 AT2G32250 28.571 406 277 5 266 666 146 543 1.69e-53 199
MS.gene32967.t1 AT2G32250 28.571 406 277 5 266 666 146 543 1.87e-53 199
MS.gene32967.t1 AT2G32250 28.571 406 277 5 266 666 187 584 1.95e-53 199
MS.gene32967.t1 AT2G32250 28.571 406 277 5 266 666 187 584 1.95e-53 199
MS.gene32967.t1 AT2G32250 28.571 406 277 5 266 666 187 584 2.02e-53 199
MS.gene32967.t1 AT2G32250 28.571 406 277 5 266 666 187 584 2.02e-53 199
MS.gene32967.t1 AT2G32250 28.571 406 277 5 266 666 187 584 2.02e-53 199
MS.gene32967.t1 AT5G28530 25.532 564 389 11 86 628 67 620 1.31e-52 195
MS.gene32967.t1 AT5G28530 25.524 572 375 13 86 628 67 616 1.60e-52 194
MS.gene32967.t1 AT5G28530 25.524 572 375 13 86 628 67 616 1.60e-52 194
MS.gene32967.t1 AT1G10240 25.623 562 392 11 80 626 54 604 1.60e-51 192
MS.gene32967.t1 AT1G10240 25.623 562 392 11 80 626 54 604 1.60e-51 192
MS.gene32967.t1 AT4G19990 24.955 553 355 11 112 643 3 516 2.92e-51 191
MS.gene32967.t1 AT1G52520 25.714 630 404 16 29 629 44 638 3.86e-48 182
MS.gene32967.t1 AT5G28530 23.188 552 354 12 86 628 67 557 6.80e-34 138
MS.gene32967.t1 AT5G28530 23.214 560 340 14 86 628 67 553 9.30e-34 138

Find 156 sgRNAs with CRISPR-Local

Find 200 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTTAGTTCTAACCCTATT+TGG 0.128040 1.1:+5544181 None:intergenic
AGTTGAGTATAGATGATTTA+TGG 0.151163 1.1:-5546112 MS.gene32967:CDS
GTTTGAATTACATGAGATTT+AGG 0.165065 1.1:-5544837 MS.gene32967:CDS
AGATGACAAATTATGATATT+TGG 0.232106 1.1:-5545746 MS.gene32967:CDS
CATGCACTGTTATTGATTCT+AGG 0.243686 1.1:-5544675 MS.gene32967:CDS
GAAGGCCTTCACGCACAATT+TGG 0.260435 1.1:-5544916 MS.gene32967:CDS
AGGGAAGTAGAAGCAGATTT+TGG 0.266967 1.1:-5544817 MS.gene32967:CDS
GCAACCTCTTCTGTTGTTCC+AGG 0.291028 1.1:+5546215 None:intergenic
AACGAGGAGTTTGGCGATAT+CGG 0.298205 1.1:-5543980 MS.gene32967:CDS
AGCAGACCTTTGTATGTTAT+AGG 0.299097 1.1:-5545971 MS.gene32967:CDS
AAAATACAATACTATGATTA+TGG 0.299516 1.1:+5545352 None:intergenic
GAAGGCTTTCACATTTGTTT+TGG 0.311863 1.1:-5545491 MS.gene32967:CDS
CCTATACTTGAGAGTGTTGC+TGG 0.316186 1.1:-5544757 MS.gene32967:CDS
GAATCCAATGCCATTGATAA+TGG 0.319184 1.1:-5544016 MS.gene32967:CDS
AGAAATCAAGCAAATTGTTT+TGG 0.327261 1.1:+5544878 None:intergenic
TTTAACTTGTTATGTTTATA+CGG 0.327417 1.1:-5544647 MS.gene32967:CDS
TTTGAATTACATGAGATTTA+GGG 0.329750 1.1:-5544836 MS.gene32967:CDS
TTGCTTATTATGTAGGATTT+GGG 0.330556 1.1:-5546281 MS.gene32967:intron
ATCCGTGGTAAATTCTTTGC+TGG 0.332956 1.1:-5544961 MS.gene32967:CDS
TTTACCATGGTCCCTTTGTT+TGG 0.333858 1.1:+5544129 None:intergenic
AGACACTGCTGAATCCAAAT+AGG 0.334078 1.1:-5544195 MS.gene32967:CDS
ACCCAAGAGCACATGTAATA+TGG 0.338001 1.1:-5544615 MS.gene32967:CDS
CGATATCGGAAATGTTGATA+TGG 0.343004 1.1:-5543966 MS.gene32967:intron
GGCAAAGGTGTTTCTAAAAC+AGG 0.346039 1.1:+5544778 None:intergenic
TGCCATATTACATGTGCTCT+TGG 0.350833 1.1:+5544613 None:intergenic
ATACAAAGGTCTGCTGAATT+AGG 0.354056 1.1:+5545979 None:intergenic
AAGATATTCACACGCGGCTT+TGG 0.358026 1.1:+5545572 None:intergenic
TCCTCAACAAAGGCAATATT+AGG 0.358068 1.1:+5544217 None:intergenic
TTAGCGATGAAACAATGTTT+TGG 0.375310 1.1:-5545536 MS.gene32967:CDS
CCGGAAGCATTCCCTTGAAT+TGG 0.376341 1.1:+5545778 None:intergenic
TTTGAACATCGTAACCTTGC+CGG 0.386902 1.1:-5544491 MS.gene32967:CDS
ATTGATGAGAACGAGGAGTT+TGG 0.387151 1.1:-5543989 MS.gene32967:CDS
GAACATGCTCTCCGTAGAAT+TGG 0.400568 1.1:+5544697 None:intergenic
AAGGCCTTCACGCACAATTT+GGG 0.401284 1.1:-5544915 MS.gene32967:CDS
CGGCATAAGGTTGATGATAA+AGG 0.401985 1.1:-5545513 MS.gene32967:CDS
CCGCACCTCACACGAGCTCT+TGG 0.404430 1.1:+5545897 None:intergenic
GATATGATGGTCACTGAGTT+TGG 0.407993 1.1:-5545048 MS.gene32967:CDS
AAATTCAGAAATTGTATGTT+GGG 0.414460 1.1:-5545105 MS.gene32967:CDS
TTGGACTGGAAAATCACAAA+TGG 0.423074 1.1:-5545029 MS.gene32967:CDS
CACTTGAAGGTGAGACTAGT+TGG 0.424002 1.1:+5544577 None:intergenic
GTTCTATCTCAAAATCCATT+TGG 0.424686 1.1:-5544049 MS.gene32967:CDS
TGTTGGGTGACTACGATGTT+GGG 0.425474 1.1:-5545089 MS.gene32967:CDS
CCAATTCAAGGGAATGCTTC+CGG 0.427188 1.1:-5545778 MS.gene32967:CDS
TGAACACGTGATAGCCGTTT+TGG 0.429315 1.1:-5544518 MS.gene32967:CDS
GGAAAATCACAAATGGGTTA+AGG 0.431588 1.1:-5545022 MS.gene32967:CDS
CCTTTCGCATGAGCACTTGA+AGG 0.445772 1.1:+5544564 None:intergenic
CCAAATAGGGTTAGAACTAA+AGG 0.448859 1.1:-5544181 MS.gene32967:CDS
TTGGGGAGTTTAAGCGTAAG+TGG 0.449176 1.1:-5545071 MS.gene32967:CDS
GTGGTTACTTGAACAACTAT+TGG 0.453156 1.1:-5545289 MS.gene32967:CDS
TGTGGTATATGTAGGATTGT+AGG 0.460432 1.1:-5544094 MS.gene32967:CDS
GCCATATTACATGTGCTCTT+GGG 0.461414 1.1:+5544614 None:intergenic
CCAGCAACACTCTCAAGTAT+AGG 0.471863 1.1:+5544757 None:intergenic
TTGGCAATGAGAAAAGCTAT+TGG 0.473791 1.1:-5545219 MS.gene32967:CDS
GAGGTTGCAGATGGCACTAA+TGG 0.478378 1.1:-5546200 MS.gene32967:CDS
GACACTGCTGAATCCAAATA+GGG 0.480012 1.1:-5544194 MS.gene32967:CDS
GATTCAGCTTGATTACCAAA+TGG 0.482585 1.1:+5544034 None:intergenic
GGTTTGTTTGCTAATGAAGC+TGG 0.482853 1.1:-5545675 MS.gene32967:CDS
GGCAACTGCCGTGAATTGCT+TGG 0.487437 1.1:-5546162 MS.gene32967:CDS
AATGTTGGATGACCAATTCA+AGG 0.489800 1.1:-5545790 MS.gene32967:CDS
ATCAATGTGTCCATTATCAA+TGG 0.492096 1.1:+5544006 None:intergenic
AATGTCTTCCAAGCAATTCA+CGG 0.494836 1.1:+5546154 None:intergenic
TCTTATTCCAACCAATTCTA+CGG 0.498289 1.1:-5544708 MS.gene32967:CDS
TTTGTCATCTTCCTATGTGC+CGG 0.501947 1.1:+5545759 None:intergenic
TGGACTGGAAAATCACAAAT+GGG 0.503516 1.1:-5545028 MS.gene32967:CDS
CCTTTGTATGTTATAGGCAA+GGG 0.503564 1.1:-5545965 MS.gene32967:CDS
CAAAGAATTTACCACGGATG+TGG 0.505462 1.1:+5544965 None:intergenic
GTGTCCATTATCAATGGCAT+TGG 0.506607 1.1:+5544012 None:intergenic
TCATAATCGCAAGTCTTGTC+CGG 0.508285 1.1:-5544071 MS.gene32967:CDS
TGAACAACTATTGGAAGCAA+TGG 0.514794 1.1:-5545280 MS.gene32967:CDS
GCCGCAAAAGCAAAGTTGTA+AGG 0.517061 1.1:-5546016 MS.gene32967:CDS
ATACTTGTTCCTCTTGTATG+TGG 0.519775 1.1:+5545410 None:intergenic
AAAATTCAGAAATTGTATGT+TGG 0.523468 1.1:-5545106 MS.gene32967:CDS
CACAAACTTGTGGTATATGT+AGG 0.523563 1.1:-5544102 MS.gene32967:CDS
ACCTTTGTATGTTATAGGCA+AGG 0.523646 1.1:-5545966 MS.gene32967:CDS
CACTAATGGTAACAAATTTG+AGG 0.523859 1.1:-5546186 MS.gene32967:CDS
AATGAGCATAGTTATGATCC+TGG 0.524091 1.1:-5546233 MS.gene32967:CDS
CCTTCAAGTGCTCATGCGAA+AGG 0.525167 1.1:-5544564 MS.gene32967:CDS
CTCCAGAAAATACAACCAAT+GGG 0.526122 1.1:+5545382 None:intergenic
TGCGAAAGGTTTGAGTCCCT+CGG 0.526391 1.1:-5544550 MS.gene32967:CDS
ACACAATCTGTGGTGACACT+TGG 0.527185 1.1:+5545185 None:intergenic
CACAATCTGTGGTGACACTT+GGG 0.528250 1.1:+5545186 None:intergenic
ACCTAATATTGCCTTTGTTG+AGG 0.528635 1.1:-5544218 MS.gene32967:CDS
GAACAACTATTGGAAGCAAT+GGG 0.533141 1.1:-5545279 MS.gene32967:CDS
GATGTTCAAATACACCAAAA+CGG 0.533380 1.1:+5544504 None:intergenic
TAAGCGTAAGTGGGATATGA+TGG 0.536902 1.1:-5545061 MS.gene32967:CDS
AAATTGATTATTGTTGACAT+TGG 0.538470 1.1:+5545134 None:intergenic
GGGAAATTTGTTATTGTCTG+AGG 0.539782 1.1:-5546261 MS.gene32967:CDS
GAACAAGTATTTGTGCCCAT+TGG 0.543961 1.1:-5545397 MS.gene32967:CDS
ACTCCAGAAAATACAACCAA+TGG 0.549257 1.1:+5545381 None:intergenic
ATGTTGGGTGACTACGATGT+TGG 0.550614 1.1:-5545090 MS.gene32967:CDS
TGAACATAATCATTCAATGT+TGG 0.554622 1.1:-5545805 MS.gene32967:CDS
TGTACGATAAGCGTCATATG+TGG 0.557922 1.1:-5544996 MS.gene32967:CDS
AGTCATAACTGATGGTGACT+TGG 0.558049 1.1:-5545238 MS.gene32967:CDS
AGGGTTAGAACTAAAGGATG+TGG 0.560571 1.1:-5544175 MS.gene32967:CDS
TTGTTTGCTAATGAAGCTGG+TGG 0.564693 1.1:-5545672 MS.gene32967:CDS
CTTCACATCGAGAAGTTGTA+CGG 0.567284 1.1:+5544935 None:intergenic
ATGAGCAACATGCGACAAGT+TGG 0.567690 1.1:-5545720 MS.gene32967:CDS
GTGCATATTGATGTAAATAG+TGG 0.570026 1.1:-5545855 MS.gene32967:CDS
ATGCTCTCCGTAGAATTGGT+TGG 0.571506 1.1:+5544701 None:intergenic
ATGTGGTTGAACTATGTAAG+AGG 0.575654 1.1:-5544360 MS.gene32967:CDS
TCACCCAGTCGATTCACCAC+AGG 0.578294 1.1:-5546375 MS.gene32967:intron
TGAAACAAGGCTATCCGGCA+AGG 0.583237 1.1:+5544477 None:intergenic
AACTATTGGAAGCAATGGGT+GGG 0.587808 1.1:-5545275 MS.gene32967:CDS
AATGTACTACGCAAAAGTGT+AGG 0.588834 1.1:+5544307 None:intergenic
AGGGAATGCTTCCGGCACAT+AGG 0.589796 1.1:-5545770 MS.gene32967:CDS
CAAACCTGTGGTGAATCGAC+TGG 0.589959 1.1:+5546371 None:intergenic
GATGGTCACTGAGTTTGGAC+TGG 0.590126 1.1:-5545043 MS.gene32967:CDS
CAACTATTGGAAGCAATGGG+TGG 0.590247 1.1:-5545276 MS.gene32967:CDS
TGGGGAGTTTAAGCGTAAGT+GGG 0.593817 1.1:-5545070 MS.gene32967:CDS
CGTGTTCACATGCGATGCCG+AGG 0.593869 1.1:+5544533 None:intergenic
GGTTCTAGAAAATGCATGCA+AGG 0.596751 1.1:-5544260 MS.gene32967:CDS
ATGTTGGATGACCAATTCAA+GGG 0.598320 1.1:-5545789 MS.gene32967:CDS
CCCTTGCCTATAACATACAA+AGG 0.598990 1.1:+5545965 None:intergenic
AAAGAATTTACCACGGATGT+GGG 0.599554 1.1:+5544966 None:intergenic
GTCGTGGCAGCTGTGAAGTG+TGG 0.599925 1.1:-5544334 MS.gene32967:CDS
ACCTCCCAAATTGTGCGTGA+AGG 0.601483 1.1:+5544911 None:intergenic
TTGCTGGTGAGCTGTATACA+AGG 0.604141 1.1:-5544741 MS.gene32967:CDS
GGTAAGTCAGTTTACACATG+TGG 0.605810 1.1:-5544377 MS.gene32967:CDS
GGGATTAGTAAAGAGAAAAG+TGG 0.608343 1.1:-5545945 MS.gene32967:CDS
ACAATCTGTGGTGACACTTG+GGG 0.609322 1.1:+5545187 None:intergenic
GTCACCACAGATTGTGTGCA+TGG 0.614597 1.1:-5545179 MS.gene32967:CDS
CCTACGTCAGTCATAACTGA+TGG 0.619011 1.1:-5545246 MS.gene32967:CDS
CGTACAACTTCTCGATGTGA+AGG 0.624034 1.1:-5544934 MS.gene32967:CDS
GCCTTCACGCACAATTTGGG+AGG 0.625740 1.1:-5544912 MS.gene32967:CDS
GTACGATAAGCGTCATATGT+GGG 0.627193 1.1:-5544995 MS.gene32967:CDS
ATAAGGTTGATGATAAAGGA+AGG 0.635329 1.1:-5545509 MS.gene32967:CDS
GTAAACTGACTTACCATGGT+AGG 0.636455 1.1:+5544385 None:intergenic
GTTGGGTGACTACGATGTTG+GGG 0.638884 1.1:-5545088 MS.gene32967:CDS
TGGGCTACTGCCCACATCCG+TGG 0.639851 1.1:-5544976 MS.gene32967:CDS
TCGTGGCAGCTGTGAAGTGT+GGG 0.643707 1.1:-5544333 MS.gene32967:CDS
ACAACAGAAGAGGTTGCAGA+TGG 0.645232 1.1:-5546209 MS.gene32967:CDS
ACACTCTCAAGTATAGGCAA+AGG 0.646441 1.1:+5544763 None:intergenic
CAGCAGTGTCTCCTCAACAA+AGG 0.649281 1.1:+5544207 None:intergenic
CCAAGAGCTCGTGTGAGGTG+CGG 0.650655 1.1:-5545897 MS.gene32967:CDS
AAACCTGTGGTGAATCGACT+GGG 0.650947 1.1:+5546372 None:intergenic
ATGTGTAAACTGACTTACCA+TGG 0.652139 1.1:+5544381 None:intergenic
TTTAATTGAATCTCACACCA+AGG 0.652944 1.1:-5544281 MS.gene32967:CDS
AAAACCAAACAAAGGGACCA+TGG 0.653474 1.1:-5544133 MS.gene32967:CDS
TGATCCTGGAACAACAGAAG+AGG 0.655464 1.1:-5546219 MS.gene32967:CDS
ATATGTACAATGAACAAGAG+AGG 0.660521 1.1:-5545620 MS.gene32967:CDS
TAATGGTAACAAATTTGAGG+AGG 0.661974 1.1:-5546183 MS.gene32967:CDS
GTGTTCACATGCGATGCCGA+GGG 0.663133 1.1:+5544534 None:intergenic
AAAGTTGTAAGGAACAACAA+AGG 0.671963 1.1:-5546005 MS.gene32967:CDS
AAACAGAACATACATATGTG+TGG 0.676472 1.1:-5545308 MS.gene32967:CDS
GGTTGAACTATGTAAGAGGA+TGG 0.684369 1.1:-5544356 MS.gene32967:CDS
TTGATGTAAATAGTGGTCGG+TGG 0.686103 1.1:-5545848 MS.gene32967:CDS
TGTTTCAAATGATCCTACCA+TGG 0.691975 1.1:-5544398 MS.gene32967:CDS
ACTATGTAAGAGGATGGTCG+TGG 0.694774 1.1:-5544350 MS.gene32967:CDS
GATGCCATGCACACAATCTG+TGG 0.697895 1.1:+5545175 None:intergenic
ATCCAGCAAAGAATTTACCA+CGG 0.708229 1.1:+5544959 None:intergenic
TGGACACATTGATGAGAACG+AGG 0.714782 1.1:-5543996 MS.gene32967:CDS
ATATTGATGTAAATAGTGGT+CGG 0.715441 1.1:-5545851 MS.gene32967:CDS
CCATCAGTTATGACTGACGT+AGG 0.724351 1.1:+5545246 None:intergenic
GTGTGCCAAGAGCTCGTGTG+AGG 0.742198 1.1:-5545902 MS.gene32967:CDS
GCTTTCAAGATATTCACACG+CGG 0.773852 1.1:+5545566 None:intergenic
TGCTGGTGAGCTGTATACAA+GGG 0.788960 1.1:-5544740 MS.gene32967:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATACAATACTATGATTA+TGG + chr1.1:5544913-5544932 None:intergenic 15.0%
!! ACATACAAAAAACAAATATA+AGG + chr1.1:5543928-5543947 None:intergenic 15.0%
!!! TATTTTGTAGTTGTTAAAAA+TGG - chr1.1:5546360-5546379 MS.gene32967:intron 15.0%
!!! TTTAACTTGTTATGTTTATA+CGG - chr1.1:5545615-5545634 MS.gene32967:CDS 15.0%
!! AAAATTCAGAAATTGTATGT+TGG - chr1.1:5545156-5545175 MS.gene32967:CDS 20.0%
!! AAATTCAGAAATTGTATGTT+GGG - chr1.1:5545157-5545176 MS.gene32967:CDS 20.0%
!! AAATTGATTATTGTTGACAT+TGG + chr1.1:5545131-5545150 None:intergenic 20.0%
!! AGATGACAAATTATGATATT+TGG - chr1.1:5544516-5544535 MS.gene32967:CDS 20.0%
!! ATTTCAATTGCTTATTATGT+AGG - chr1.1:5543974-5543993 MS.gene32967:CDS 20.0%
!! TACAAAAAACAAATATAAGG+AGG + chr1.1:5543925-5543944 None:intergenic 20.0%
!! TTTGAATTACATGAGATTTA+GGG - chr1.1:5545426-5545445 MS.gene32967:CDS 20.0%
!!! TCATTTGAAACATCATTTTT+AGG + chr1.1:5545856-5545875 None:intergenic 20.0%
! AGTTGAGTATAGATGATTTA+TGG - chr1.1:5544150-5544169 MS.gene32967:CDS 25.0%
! ATATTGATGTAAATAGTGGT+CGG - chr1.1:5544411-5544430 MS.gene32967:CDS 25.0%
! ATTGCTTATTATGTAGGATT+TGG - chr1.1:5543980-5543999 MS.gene32967:CDS 25.0%
! GTTTGAATTACATGAGATTT+AGG - chr1.1:5545425-5545444 MS.gene32967:CDS 25.0%
! TAGCAAACAAACCAAAAATA+TGG + chr1.1:5544580-5544599 None:intergenic 25.0%
! TGAACATAATCATTCAATGT+TGG - chr1.1:5544457-5544476 MS.gene32967:CDS 25.0%
! TTGCTTATTATGTAGGATTT+GGG - chr1.1:5543981-5544000 MS.gene32967:CDS 25.0%
! TTTGTGTTTGTAAATTTGCA+GGG - chr1.1:5546394-5546413 MS.gene32967:CDS 25.0%
!! AGAAATCAAGCAAATTGTTT+TGG + chr1.1:5545387-5545406 None:intergenic 25.0%
!! AGATATCATTTTGTAGATGT+TGG - chr1.1:5544173-5544192 MS.gene32967:CDS 25.0%
!! ATCAAGACAAACCATATTTT+TGG - chr1.1:5544566-5544585 MS.gene32967:CDS 25.0%
!! CAAAGAGATTATGAGATTTT+TGG - chr1.1:5544809-5544828 MS.gene32967:CDS 25.0%
!! GATTGAATCTTTTGCATTTT+TGG + chr1.1:5545821-5545840 None:intergenic 25.0%
!!! AAGTTTGTGTTTTTTTACCA+TGG + chr1.1:5546149-5546168 None:intergenic 25.0%
!!! GATTTCAAAAAGTTGGTTTT+CGG - chr1.1:5544612-5544631 MS.gene32967:CDS 25.0%
AAACAGAACATACATATGTG+TGG - chr1.1:5544954-5544973 MS.gene32967:CDS 30.0%
AAAGTTGTAAGGAACAACAA+AGG - chr1.1:5544257-5544276 MS.gene32967:CDS 30.0%
ATATGTACAATGAACAAGAG+AGG - chr1.1:5544642-5544661 MS.gene32967:CDS 30.0%
ATCAATGTGTCCATTATCAA+TGG + chr1.1:5546259-5546278 None:intergenic 30.0%
CACTAATGGTAACAAATTTG+AGG - chr1.1:5544076-5544095 MS.gene32967:CDS 30.0%
GATGTTCAAATACACCAAAA+CGG + chr1.1:5545761-5545780 None:intergenic 30.0%
GGTAAAAAAACACAAACTTG+TGG - chr1.1:5546150-5546169 MS.gene32967:CDS 30.0%
GTGCATATTGATGTAAATAG+TGG - chr1.1:5544407-5544426 MS.gene32967:CDS 30.0%
GTTCTATCTCAAAATCCATT+TGG - chr1.1:5546213-5546232 MS.gene32967:CDS 30.0%
GTTTGTGTTTGTAAATTTGC+AGG - chr1.1:5546393-5546412 MS.gene32967:CDS 30.0%
TAATGGTAACAAATTTGAGG+AGG - chr1.1:5544079-5544098 MS.gene32967:CDS 30.0%
TCTTATTCCAACCAATTCTA+CGG - chr1.1:5545554-5545573 MS.gene32967:CDS 30.0%
TTTAATTGAATCTCACACCA+AGG - chr1.1:5545981-5546000 MS.gene32967:CDS 30.0%
! CATAGTATTGTATTTTGTGC+TGG - chr1.1:5544917-5544936 MS.gene32967:CDS 30.0%
! TGGCGTTTCTATTTTACAAT+TGG - chr1.1:5544198-5544217 MS.gene32967:CDS 30.0%
! TTAGCGATGAAACAATGTTT+TGG - chr1.1:5544726-5544745 MS.gene32967:CDS 30.0%
!! ATAAGGTTGATGATAAAGGA+AGG - chr1.1:5544753-5544772 MS.gene32967:CDS 30.0%
!! ATAGCCTTGTTTCAAAAAGA+TGG - chr1.1:5545794-5545813 MS.gene32967:CDS 30.0%
!!! AATGGATTTTGAGATAGAAC+CGG + chr1.1:5546213-5546232 None:intergenic 30.0%
AAAGAATTTACCACGGATGT+GGG + chr1.1:5545299-5545318 None:intergenic 35.0%
AACCGCAAAAACCAAACAAA+GGG - chr1.1:5546122-5546141 MS.gene32967:CDS 35.0%
AATGAGCATAGTTATGATCC+TGG - chr1.1:5544029-5544048 MS.gene32967:CDS 35.0%
AATGTCTTCCAAGCAATTCA+CGG + chr1.1:5544111-5544130 None:intergenic 35.0%
AATGTTGGATGACCAATTCA+AGG - chr1.1:5544472-5544491 MS.gene32967:CDS 35.0%
ACCTAATATTGCCTTTGTTG+AGG - chr1.1:5546044-5546063 MS.gene32967:CDS 35.0%
ACCTTTGTATGTTATAGGCA+AGG - chr1.1:5544296-5544315 MS.gene32967:CDS 35.0%
ACTCCAGAAAATACAACCAA+TGG + chr1.1:5544884-5544903 None:intergenic 35.0%
AGCAGACCTTTGTATGTTAT+AGG - chr1.1:5544291-5544310 MS.gene32967:CDS 35.0%
ATACAAAGGTCTGCTGAATT+AGG + chr1.1:5544286-5544305 None:intergenic 35.0%
ATACTTGTTCCTCTTGTATG+TGG + chr1.1:5544855-5544874 None:intergenic 35.0%
ATCCAGCAAAGAATTTACCA+CGG + chr1.1:5545306-5545325 None:intergenic 35.0%
ATGTGGTTGAACTATGTAAG+AGG - chr1.1:5545902-5545921 MS.gene32967:CDS 35.0%
ATGTTGGATGACCAATTCAA+GGG - chr1.1:5544473-5544492 MS.gene32967:CDS 35.0%
CACAAACTTGTGGTATATGT+AGG - chr1.1:5546160-5546179 MS.gene32967:CDS 35.0%
CCAAATAGGGTTAGAACTAA+AGG - chr1.1:5546081-5546100 MS.gene32967:CDS 35.0%
CCTTTGTATGTTATAGGCAA+GGG - chr1.1:5544297-5544316 MS.gene32967:CDS 35.0%
CGATATCGGAAATGTTGATA+TGG - chr1.1:5546296-5546315 MS.gene32967:intron 35.0%
CTCCAGAAAATACAACCAAT+GGG + chr1.1:5544883-5544902 None:intergenic 35.0%
GAACAACTATTGGAAGCAAT+GGG - chr1.1:5544983-5545002 MS.gene32967:CDS 35.0%
GAATCCAATGCCATTGATAA+TGG - chr1.1:5546246-5546265 MS.gene32967:CDS 35.0%
GATTCAGCTTGATTACCAAA+TGG + chr1.1:5546231-5546250 None:intergenic 35.0%
GTGGTTACTTGAACAACTAT+TGG - chr1.1:5544973-5544992 MS.gene32967:CDS 35.0%
TCCTCAACAAAGGCAATATT+AGG + chr1.1:5546048-5546067 None:intergenic 35.0%
TGAACAACTATTGGAAGCAA+TGG - chr1.1:5544982-5545001 MS.gene32967:CDS 35.0%
TGTGGTATATGTAGGATTGT+AGG - chr1.1:5546168-5546187 MS.gene32967:CDS 35.0%
TGTTTCAAATGATCCTACCA+TGG - chr1.1:5545864-5545883 MS.gene32967:CDS 35.0%
TTGGCAATGAGAAAAGCTAT+TGG - chr1.1:5545043-5545062 MS.gene32967:CDS 35.0%
! AATGTACTACGCAAAAGTGT+AGG + chr1.1:5545958-5545977 None:intergenic 35.0%
! AATTGGTATGCGAGTTTTCA+TGG - chr1.1:5544215-5544234 MS.gene32967:CDS 35.0%
! ATGTGTAAACTGACTTACCA+TGG + chr1.1:5545884-5545903 None:intergenic 35.0%
! ATTGGTATGCGAGTTTTCAT+GGG - chr1.1:5544216-5544235 MS.gene32967:CDS 35.0%
! GAAGGCTTTCACATTTGTTT+TGG - chr1.1:5544771-5544790 MS.gene32967:CDS 35.0%
! GGGATTAGTAAAGAGAAAAG+TGG - chr1.1:5544317-5544336 MS.gene32967:CDS 35.0%
! TCATTTTGTAGATGTTGGTG+TGG - chr1.1:5544178-5544197 MS.gene32967:CDS 35.0%
! TGGACTGGAAAATCACAAAT+GGG - chr1.1:5545234-5545253 MS.gene32967:CDS 35.0%
! TTGGACTGGAAAATCACAAA+TGG - chr1.1:5545233-5545252 MS.gene32967:CDS 35.0%
!! CATGCACTGTTATTGATTCT+AGG - chr1.1:5545587-5545606 MS.gene32967:CDS 35.0%
!! CCTTTAGTTCTAACCCTATT+TGG + chr1.1:5546084-5546103 None:intergenic 35.0%
!! GGAAAATCACAAATGGGTTA+AGG - chr1.1:5545240-5545259 MS.gene32967:CDS 35.0%
!! GGGAAATTTGTTATTGTCTG+AGG - chr1.1:5544001-5544020 MS.gene32967:CDS 35.0%
!! TCCTTACAACTTTGCTTTTG+CGG + chr1.1:5544250-5544269 None:intergenic 35.0%
!!! CTTTTTGAAACAAGGCTATC+CGG + chr1.1:5545793-5545812 None:intergenic 35.0%
!!! TCACATTTGTTTTGGTGTGA+CGG - chr1.1:5544779-5544798 MS.gene32967:CDS 35.0%
!!! TGGTCCATCTTTTTGAAACA+AGG + chr1.1:5545801-5545820 None:intergenic 35.0%
AAAACCAAACAAAGGGACCA+TGG - chr1.1:5546129-5546148 MS.gene32967:CDS 40.0%
AACTATTGGAAGCAATGGGT+GGG - chr1.1:5544987-5545006 MS.gene32967:CDS 40.0%
ACACTCTCAAGTATAGGCAA+AGG + chr1.1:5545502-5545521 None:intergenic 40.0%
AGACACTGCTGAATCCAAAT+AGG - chr1.1:5546067-5546086 MS.gene32967:CDS 40.0%
AGGGTTAGAACTAAAGGATG+TGG - chr1.1:5546087-5546106 MS.gene32967:CDS 40.0%
ATCCGTGGTAAATTCTTTGC+TGG - chr1.1:5545301-5545320 MS.gene32967:CDS 40.0%
ATTGATGAGAACGAGGAGTT+TGG - chr1.1:5546273-5546292 MS.gene32967:intron 40.0%
CAAAGAATTTACCACGGATG+TGG + chr1.1:5545300-5545319 None:intergenic 40.0%
CAACCGCAAAAACCAAACAA+AGG - chr1.1:5546121-5546140 MS.gene32967:CDS 40.0%
CCCTTGCCTATAACATACAA+AGG + chr1.1:5544300-5544319 None:intergenic 40.0%
CTTCACATCGAGAAGTTGTA+CGG + chr1.1:5545330-5545349 None:intergenic 40.0%
GAACAAGTATTTGTGCCCAT+TGG - chr1.1:5544865-5544884 MS.gene32967:CDS 40.0%
GACACTGCTGAATCCAAATA+GGG - chr1.1:5546068-5546087 MS.gene32967:CDS 40.0%
GATATGATGGTCACTGAGTT+TGG - chr1.1:5545214-5545233 MS.gene32967:CDS 40.0%
GCCATATTACATGTGCTCTT+GGG + chr1.1:5545651-5545670 None:intergenic 40.0%
GCTTTCAAGATATTCACACG+CGG + chr1.1:5544699-5544718 None:intergenic 40.0%
GGTAAGTCAGTTTACACATG+TGG - chr1.1:5545885-5545904 MS.gene32967:CDS 40.0%
GGTTGAACTATGTAAGAGGA+TGG - chr1.1:5545906-5545925 MS.gene32967:CDS 40.0%
GTACGATAAGCGTCATATGT+GGG - chr1.1:5545267-5545286 MS.gene32967:CDS 40.0%
GTGTCCATTATCAATGGCAT+TGG + chr1.1:5546253-5546272 None:intergenic 40.0%
TAAGCGTAAGTGGGATATGA+TGG - chr1.1:5545201-5545220 MS.gene32967:CDS 40.0%
TGCCATATTACATGTGCTCT+TGG + chr1.1:5545652-5545671 None:intergenic 40.0%
TGTACGATAAGCGTCATATG+TGG - chr1.1:5545266-5545285 MS.gene32967:CDS 40.0%
TTGATGTAAATAGTGGTCGG+TGG - chr1.1:5544414-5544433 MS.gene32967:CDS 40.0%
TTTACCATGGTCCCTTTGTT+TGG + chr1.1:5546136-5546155 None:intergenic 40.0%
TTTGAACATCGTAACCTTGC+CGG - chr1.1:5545771-5545790 MS.gene32967:CDS 40.0%
TTTGTCATCTTCCTATGTGC+CGG + chr1.1:5544506-5544525 None:intergenic 40.0%
! ACCCAAGAGCACATGTAATA+TGG - chr1.1:5545647-5545666 MS.gene32967:CDS 40.0%
! AGGGAAGTAGAAGCAGATTT+TGG - chr1.1:5545445-5545464 MS.gene32967:CDS 40.0%
! AGTCATAACTGATGGTGACT+TGG - chr1.1:5545024-5545043 MS.gene32967:CDS 40.0%
! CTTTTGATGCCACATACAAG+AGG - chr1.1:5544843-5544862 MS.gene32967:CDS 40.0%
! GGTTTGTTTGCTAATGAAGC+TGG - chr1.1:5544587-5544606 MS.gene32967:CDS 40.0%
! GTAAACTGACTTACCATGGT+AGG + chr1.1:5545880-5545899 None:intergenic 40.0%
! TCATAATCGCAAGTCTTGTC+CGG - chr1.1:5546191-5546210 MS.gene32967:CDS 40.0%
! TTGTTTGCTAATGAAGCTGG+TGG - chr1.1:5544590-5544609 MS.gene32967:CDS 40.0%
!! CGGCATAAGGTTGATGATAA+AGG - chr1.1:5544749-5544768 MS.gene32967:CDS 40.0%
!! GCGATGAAACAATGTTTTGG+CGG - chr1.1:5544729-5544748 MS.gene32967:CDS 40.0%
!! GCTGGTGGATTTCAAAAAGT+TGG - chr1.1:5544605-5544624 MS.gene32967:CDS 40.0%
!! GGCAAAGGTGTTTCTAAAAC+AGG + chr1.1:5545487-5545506 None:intergenic 40.0%
!! GGTTCTAGAAAATGCATGCA+AGG - chr1.1:5546002-5546021 MS.gene32967:CDS 40.0%
!! TGCCCATTGGTTGTATTTTC+TGG - chr1.1:5544878-5544897 MS.gene32967:CDS 40.0%
!!! AACAATGTTTTGGCGGCATA+AGG - chr1.1:5544736-5544755 MS.gene32967:CDS 40.0%
!!! GCAGATTTTGGTTCTAGTCA+TGG - chr1.1:5545457-5545476 MS.gene32967:CDS 40.0%
!!! GCATGCATTTTCTAGAACCT+TGG + chr1.1:5546001-5546020 None:intergenic 40.0%
!!! GTCCCTTTGTTTGGTTTTTG+CGG + chr1.1:5546127-5546146 None:intergenic 40.0%
!!! TGTTTGGTTTTTGCGGTTGA+CGG + chr1.1:5546120-5546139 None:intergenic 40.0%
AAACCTGTGGTGAATCGACT+GGG + chr1.1:5543893-5543912 None:intergenic 45.0%
AACGAGGAGTTTGGCGATAT+CGG - chr1.1:5546282-5546301 MS.gene32967:intron 45.0%
AAGATATTCACACGCGGCTT+TGG + chr1.1:5544693-5544712 None:intergenic 45.0%
AAGGCCTTCACGCACAATTT+GGG - chr1.1:5545347-5545366 MS.gene32967:CDS 45.0%
ACAACAGAAGAGGTTGCAGA+TGG - chr1.1:5544053-5544072 MS.gene32967:CDS 45.0%
ACAATCTGTGGTGACACTTG+GGG + chr1.1:5545078-5545097 None:intergenic 45.0%
ACACAATCTGTGGTGACACT+TGG + chr1.1:5545080-5545099 None:intergenic 45.0%
ACTATGTAAGAGGATGGTCG+TGG - chr1.1:5545912-5545931 MS.gene32967:CDS 45.0%
ATGAGCAACATGCGACAAGT+TGG - chr1.1:5544542-5544561 MS.gene32967:CDS 45.0%
ATGCTCTCCGTAGAATTGGT+TGG + chr1.1:5545564-5545583 None:intergenic 45.0%
ATGTTGGGTGACTACGATGT+TGG - chr1.1:5545172-5545191 MS.gene32967:CDS 45.0%
CAACTATTGGAAGCAATGGG+TGG - chr1.1:5544986-5545005 MS.gene32967:CDS 45.0%
CACAATCTGTGGTGACACTT+GGG + chr1.1:5545079-5545098 None:intergenic 45.0%
CACTTGAAGGTGAGACTAGT+TGG + chr1.1:5545688-5545707 None:intergenic 45.0%
CCAATTCAAGGGAATGCTTC+CGG - chr1.1:5544484-5544503 MS.gene32967:CDS 45.0%
CCAGCAACACTCTCAAGTAT+AGG + chr1.1:5545508-5545527 None:intergenic 45.0%
CCATCAGTTATGACTGACGT+AGG + chr1.1:5545019-5545038 None:intergenic 45.0%
CCTACGTCAGTCATAACTGA+TGG - chr1.1:5545016-5545035 MS.gene32967:CDS 45.0%
CGTACAACTTCTCGATGTGA+AGG - chr1.1:5545328-5545347 MS.gene32967:CDS 45.0%
GAACATGCTCTCCGTAGAAT+TGG + chr1.1:5545568-5545587 None:intergenic 45.0%
GAGGAGACAATACAAACCTG+TGG + chr1.1:5543906-5543925 None:intergenic 45.0%
GCCGCAAAAGCAAAGTTGTA+AGG - chr1.1:5544246-5544265 MS.gene32967:CDS 45.0%
TGATCCTGGAACAACAGAAG+AGG - chr1.1:5544043-5544062 MS.gene32967:CDS 45.0%
TGCTGGTGAGCTGTATACAA+GGG - chr1.1:5545522-5545541 MS.gene32967:CDS 45.0%
TGGACACATTGATGAGAACG+AGG - chr1.1:5546266-5546285 MS.gene32967:CDS 45.0%
TGGGGAGTTTAAGCGTAAGT+GGG - chr1.1:5545192-5545211 MS.gene32967:CDS 45.0%
TGTTGGGTGACTACGATGTT+GGG - chr1.1:5545173-5545192 MS.gene32967:CDS 45.0%
TTGCTGGTGAGCTGTATACA+AGG - chr1.1:5545521-5545540 MS.gene32967:CDS 45.0%
TTGGGGAGTTTAAGCGTAAG+TGG - chr1.1:5545191-5545210 MS.gene32967:CDS 45.0%
! TGAACACGTGATAGCCGTTT+TGG - chr1.1:5545744-5545763 MS.gene32967:CDS 45.0%
!! CCTATACTTGAGAGTGTTGC+TGG - chr1.1:5545505-5545524 MS.gene32967:CDS 45.0%
ACCTCCCAAATTGTGCGTGA+AGG + chr1.1:5545354-5545373 None:intergenic 50.0%
CAAACCTGTGGTGAATCGAC+TGG + chr1.1:5543894-5543913 None:intergenic 50.0%
CAGCAGTGTCTCCTCAACAA+AGG + chr1.1:5546058-5546077 None:intergenic 50.0%
CCTTCAAGTGCTCATGCGAA+AGG - chr1.1:5545698-5545717 MS.gene32967:CDS 50.0%
CCTTTCGCATGAGCACTTGA+AGG + chr1.1:5545701-5545720 None:intergenic 50.0%
GAAGGCCTTCACGCACAATT+TGG - chr1.1:5545346-5545365 MS.gene32967:CDS 50.0%
GAGGTTGCAGATGGCACTAA+TGG - chr1.1:5544062-5544081 MS.gene32967:CDS 50.0%
GATGCCATGCACACAATCTG+TGG + chr1.1:5545090-5545109 None:intergenic 50.0%
GATGGTCACTGAGTTTGGAC+TGG - chr1.1:5545219-5545238 MS.gene32967:CDS 50.0%
GCAACCTCTTCTGTTGTTCC+AGG + chr1.1:5544050-5544069 None:intergenic 50.0%
GTCACCACAGATTGTGTGCA+TGG - chr1.1:5545083-5545102 MS.gene32967:CDS 50.0%
GTTGGGTGACTACGATGTTG+GGG - chr1.1:5545174-5545193 MS.gene32967:CDS 50.0%
TGAAACAAGGCTATCCGGCA+AGG + chr1.1:5545788-5545807 None:intergenic 50.0%
TGCGAAAGGTTTGAGTCCCT+CGG - chr1.1:5545712-5545731 MS.gene32967:CDS 50.0%
! GAGCTCTTGGCACACGTTTT+CGG + chr1.1:5544355-5544374 None:intergenic 50.0%
! GCGAGTTTTCATGGGTTTGC+TGG - chr1.1:5544224-5544243 MS.gene32967:CDS 50.0%
!! ACACGTTTTCGGCTTGTGGA+TGG + chr1.1:5544344-5544363 None:intergenic 50.0%
!! CACGTTTTCGGCTTGTGGAT+GGG + chr1.1:5544343-5544362 None:intergenic 50.0%
!! CCGGAAGCATTCCCTTGAAT+TGG + chr1.1:5544487-5544506 None:intergenic 50.0%
AGGGAATGCTTCCGGCACAT+AGG - chr1.1:5544492-5544511 MS.gene32967:CDS 55.0%
GCCTTCACGCACAATTTGGG+AGG - chr1.1:5545350-5545369 MS.gene32967:CDS 55.0%
GGCAACTGCCGTGAATTGCT+TGG - chr1.1:5544100-5544119 MS.gene32967:CDS 55.0%
GTGTTCACATGCGATGCCGA+GGG + chr1.1:5545731-5545750 None:intergenic 55.0%
TCGTGGCAGCTGTGAAGTGT+GGG - chr1.1:5545929-5545948 MS.gene32967:CDS 55.0%
! TCACCCAGTCGATTCACCAC+AGG - chr1.1:5543887-5543906 MS.gene32967:intron 55.0%
!! TGGCACACGTTTTCGGCTTG+TGG + chr1.1:5544348-5544367 None:intergenic 55.0%
CCAAGAGCTCGTGTGAGGTG+CGG - chr1.1:5544365-5544384 MS.gene32967:CDS 60.0%
CGTGTTCACATGCGATGCCG+AGG + chr1.1:5545732-5545751 None:intergenic 60.0%
GTCGTGGCAGCTGTGAAGTG+TGG - chr1.1:5545928-5545947 MS.gene32967:CDS 60.0%
GTGTGCCAAGAGCTCGTGTG+AGG - chr1.1:5544360-5544379 MS.gene32967:CDS 60.0%
CCGCACCTCACACGAGCTCT+TGG + chr1.1:5544368-5544387 None:intergenic 65.0%
TGGGCTACTGCCCACATCCG+TGG - chr1.1:5545286-5545305 MS.gene32967:CDS 65.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 5543858 5546426 5543858 ID=MS.gene32967
chr1.1 mRNA 5543858 5546426 5543858 ID=MS.gene32967.t1;Parent=MS.gene32967
chr1.1 exon 5546376 5546426 5546376 ID=MS.gene32967.t1.exon1;Parent=MS.gene32967.t1
chr1.1 CDS 5546376 5546426 5546376 ID=cds.MS.gene32967.t1;Parent=MS.gene32967.t1
chr1.1 exon 5543967 5546288 5543967 ID=MS.gene32967.t1.exon2;Parent=MS.gene32967.t1
chr1.1 CDS 5543967 5546288 5543967 ID=cds.MS.gene32967.t1;Parent=MS.gene32967.t1
chr1.1 exon 5543858 5543869 5543858 ID=MS.gene32967.t1.exon3;Parent=MS.gene32967.t1
chr1.1 CDS 5543858 5543869 5543858 ID=cds.MS.gene32967.t1;Parent=MS.gene32967.t1
Gene Sequence

>MS.gene32967

ATGGAGGATGTTGATTCTGAATCTGTTACTCACCCAGTCGATTCACCACAGGTTTGTATTGTCTCCTCCTTATATTTGTTTTTTGTATGTTGTGATTAGTTGTTAAAAATATTAAAATTTCAATTGCTTATTATGTAGGATTTGGGAAATTTGTTATTGTCTGAGGATGAGAATGAGCATAGTTATGATCCTGGAACAACAGAAGAGGTTGCAGATGGCACTAATGGTAACAAATTTGAGGAGGCAACTGCCGTGAATTGCTTGGAAGACATTTCAAGTATCAATTTCGAAGAGTTGAGTATAGATGATTTATGGAGATATCATTTTGTAGATGTTGGTGTGGCGTTTCTATTTTACAATTGGTATGCGAGTTTTCATGGGTTTGCTGGCCGCAAAAGCAAAGTTGTAAGGAACAACAAAGGAGACCTAATTCAGCAGACCTTTGTATGTTATAGGCAAGGGATTAGTAAAGAGAAAAGTGGCCCATCCACAAGCCGAAAACGTGTGCCAAGAGCTCGTGTGAGGTGCGGTTGCGAAGCAAAGTGTCGCGTGCATATTGATGTAAATAGTGGTCGGTGGTACATGAAGTTTTTTGACGATGAACATAATCATTCAATGTTGGATGACCAATTCAAGGGAATGCTTCCGGCACATAGGAAGATGACAAATTATGATATTTGGCGAATGAGCAACATGCGACAAGTTGGTATCAAGACAAACCATATTTTTGGTTTGTTTGCTAATGAAGCTGGTGGATTTCAAAAAGTTGGTTTTCGGAAGCAAGATATGTACAATGAACAAGAGAGGCAGCGATTAACTTCTTCTAAAGATGCCAAAGCCGCGTGTGAATATCTTGAAAGCTTGTGCGTTAGCGATGAAACAATGTTTTGGCGGCATAAGGTTGATGATAAAGGAAGGCTTTCACATTTGTTTTGGTGTGACGGTGCTGCACAAAGAGATTATGAGATTTTTGGTGATGTAGTTGCTTTTGATGCCACATACAAGAGGAACAAGTATTTGTGCCCATTGGTTGTATTTTCTGGAGTGAATCACCATAATCATAGTATTGTATTTTGTGCTGGTATTGTTTGTGATGAAACAGAACATACATATGTGTGGTTACTTGAACAACTATTGGAAGCAATGGGTGGGAAAAGTCCTACGTCAGTCATAACTGATGGTGACTTGGCAATGAGAAAAGCTATTGGAAAAGTTTTCCCCAAGTGTCACCACAGATTGTGTGCATGGCATCTGCTACGTAACGCTTCGACCAATGTCAACAATAATCAATTTGTGCTAAAATTCAGAAATTGTATGTTGGGTGACTACGATGTTGGGGAGTTTAAGCGTAAGTGGGATATGATGGTCACTGAGTTTGGACTGGAAAATCACAAATGGGTTAAGGAAATGTACGATAAGCGTCATATGTGGGCTACTGCCCACATCCGTGGTAAATTCTTTGCTGGATTCCGTACAACTTCTCGATGTGAAGGCCTTCACGCACAATTTGGGAGGTATGTTAACTACCAAAACAATTTGCTTGATTTCTTGAAACAATATTTTAGATGTTTGAATTACATGAGATTTAGGGAAGTAGAAGCAGATTTTGGTTCTAGTCATGGTGAACCTGTTTTAGAAACACCTTTGCCTATACTTGAGAGTGTTGCTGGTGAGCTGTATACAAGGGAGATGTTTCTCTTATTCCAACCAATTCTACGGAGAGCATGTTCATGCACTGTTATTGATTCTAGGAAAACTTTAACTTGTTATGTTTATACGGTCAGTAGATACCCAAGAGCACATGTAATATGGCAAGTTTCATTTTCTCCAACTAGTCTCACCTTCAAGTGCTCATGCGAAAGGTTTGAGTCCCTCGGCATCGCATGTGAACACGTGATAGCCGTTTTGGTGTATTTGAACATCGTAACCTTGCCGGATAGCCTTGTTTCAAAAAGATGGACCAAAAATGCAAAAGATTCAATCACTGTTTGTACACCTAAAAATGATGTTTCAAATGATCCTACCATGGTAAGTCAGTTTACACATGTGGTTGAACTATGTAAGAGGATGGTCGTGGCAGCTGTGAAGTGTGGGAAGCCTACACTTTTGCGTAGTACATTTGATTTAATTGAATCTCACACCAAGGTTCTAGAAAATGCATGCAAGGACGTTGATTCATCATATGTACCTAATATTGCCTTTGTTGAGGAGACACTGCTGAATCCAAATAGGGTTAGAACTAAAGGATGTGGTGCAACACCGTCAACCGCAAAAACCAAACAAAGGGACCATGGTAAAAAAACACAAACTTGTGGTATATGTAGGATTGTAGGTCATAATCGCAAGTCTTGTCCGGTTCTATCTCAAAATCCATTTGGTAATCAAGCTGAATCCAATGCCATTGATAATGGACACATTGATGAGAACGAGGAGTTTGGCGATATCGGAAATGTTGATATGGTTAGTTCTTTTTCACAAACGTACAAGATATTTATGATGCATTATTTTGTAGTTGTTAAAAATGGTTGACACTCTGTTTGTGTTTGTAAATTTGCAGGGTATGTATTGA
Protein sequence

>MS.gene32967.t1

MEDVDSESVTHPVDSPQDLGNLLLSEDENEHSYDPGTTEEVADGTNGNKFEEATAVNCLEDISSINFEELSIDDLWRYHFVDVGVAFLFYNWYASFHGFAGRKSKVVRNNKGDLIQQTFVCYRQGISKEKSGPSTSRKRVPRARVRCGCEAKCRVHIDVNSGRWYMKFFDDEHNHSMLDDQFKGMLPAHRKMTNYDIWRMSNMRQVGIKTNHIFGLFANEAGGFQKVGFRKQDMYNEQERQRLTSSKDAKAACEYLESLCVSDETMFWRHKVDDKGRLSHLFWCDGAAQRDYEIFGDVVAFDATYKRNKYLCPLVVFSGVNHHNHSIVFCAGIVCDETEHTYVWLLEQLLEAMGGKSPTSVITDGDLAMRKAIGKVFPKCHHRLCAWHLLRNASTNVNNNQFVLKFRNCMLGDYDVGEFKRKWDMMVTEFGLENHKWVKEMYDKRHMWATAHIRGKFFAGFRTTSRCEGLHAQFGRYVNYQNNLLDFLKQYFRCLNYMRFREVEADFGSSHGEPVLETPLPILESVAGELYTREMFLLFQPILRRACSCTVIDSRKTLTCYVYTVSRYPRAHVIWQVSFSPTSLTFKCSCERFESLGIACEHVIAVLVYLNIVTLPDSLVSKRWTKNAKDSITVCTPKNDVSNDPTMVSQFTHVVELCKRMVVAAVKCGKPTLLRSTFDLIESHTKVLENACKDVDSSYVPNIAFVEETLLNPNRVRTKGCGATPSTAKTKQRDHGKKTQTCGICRIVGHNRKSCPVLSQNPFGNQAESNAIDNGHIDENEEFGDIGNVDMGMY