Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33000.t1 | AES59248.1 | 89.1 | 55 | 6 | 0 | 1 | 55 | 50 | 104 | 3.60E-20 | 107.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33000.t1 | G7I9Y7 | 89.1 | 55 | 6 | 0 | 1 | 55 | 50 | 104 | 2.6e-20 | 107.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene33000.t1 | TF | C2C2-GATA |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33000.t1 | MTR_1g016200 | 89.091 | 55 | 6 | 0 | 1 | 55 | 50 | 104 | 3.77e-32 | 107 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 19 sgRNAs with CRISPR-Local
Find 24 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAATGAGAGTAAGCAATATT+TGG | 0.256438 | 1.1:+5947566 | None:intergenic |
| TTGAGTATCAAGACCCTTAT+CGG | 0.292091 | 1.1:-5947837 | None:intergenic |
| ATACAAATACTACTTCTCTT+TGG | 0.332697 | 1.1:+5947617 | MS.gene33000:CDS |
| TCTTGATACTCAAAAGGAAA+AGG | 0.439670 | 1.1:+5947847 | MS.gene33000:CDS |
| TTTCCTCTTCCCTTCTTCGT+CGG | 0.486411 | 1.1:-5947811 | None:intergenic |
| ACTCAAAAGGAAAAGGGAAA+TGG | 0.486925 | 1.1:+5947854 | MS.gene33000:CDS |
| CAAATACTACTTCTCTTTGG+AGG | 0.495243 | 1.1:+5947620 | MS.gene33000:CDS |
| TAAGGGTCTTGATACTCAAA+AGG | 0.499945 | 1.1:+5947841 | MS.gene33000:CDS |
| CTTGATACTCAAAAGGAAAA+GGG | 0.500087 | 1.1:+5947848 | MS.gene33000:CDS |
| TCTTTGGAGGAGTGATCCTA+CGG | 0.509509 | 1.1:+5947633 | MS.gene33000:CDS |
| ATGTGGACTCCGACGAAGAA+GGG | 0.537059 | 1.1:+5947802 | MS.gene33000:CDS |
| GAGTGATCCTACGGGTTCCA+AGG | 0.561497 | 1.1:+5947642 | MS.gene33000:CDS |
| CTTTGGAGGAGTGATCCTAC+GGG | 0.578542 | 1.1:+5947634 | MS.gene33000:CDS |
| CATGTGGACTCCGACGAAGA+AGG | 0.582374 | 1.1:+5947801 | MS.gene33000:CDS |
| TGTATCGCAATCGAAACAAA+CGG | 0.590406 | 1.1:-5947599 | None:intergenic |
| ACTCCGACGAAGAAGGGAAG+AGG | 0.605566 | 1.1:+5947808 | MS.gene33000:CDS |
| TGGCGAAAGCTCATCATCTG+AGG | 0.622295 | 1.1:+5947874 | MS.gene33000:CDS |
| CAGTGGTTGTGTAACGCATG+TGG | 0.661654 | 1.1:+5947785 | MS.gene33000:intron |
| CTAATTACCTTGGAACCCGT+AGG | 0.693638 | 1.1:-5947649 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAGTTAACAAGAAATATGT+TGG | + | chr1.1:5947760-5947779 | MS.gene33000:intron | 20.0% |
| ! | ATACAAATACTACTTCTCTT+TGG | + | chr1.1:5947617-5947636 | MS.gene33000:CDS | 25.0% |
| !!! | CTACTATCAACTTTAACTTT+TGG | + | chr1.1:5947685-5947704 | MS.gene33000:intron | 25.0% |
| AAGAGATGAACTAATTACCT+TGG | - | chr1.1:5947662-5947681 | None:intergenic | 30.0% | |
| CTTGATACTCAAAAGGAAAA+GGG | + | chr1.1:5947848-5947867 | MS.gene33000:CDS | 30.0% | |
| TCTTGATACTCAAAAGGAAA+AGG | + | chr1.1:5947847-5947866 | MS.gene33000:CDS | 30.0% | |
| ACTCAAAAGGAAAAGGGAAA+TGG | + | chr1.1:5947854-5947873 | MS.gene33000:CDS | 35.0% | |
| CAAATACTACTTCTCTTTGG+AGG | + | chr1.1:5947620-5947639 | MS.gene33000:CDS | 35.0% | |
| TAAGGGTCTTGATACTCAAA+AGG | + | chr1.1:5947841-5947860 | MS.gene33000:CDS | 35.0% | |
| TGTATCGCAATCGAAACAAA+CGG | - | chr1.1:5947602-5947621 | None:intergenic | 35.0% | |
| TTGAGTATCAAGACCCTTAT+CGG | - | chr1.1:5947840-5947859 | None:intergenic | 35.0% | |
| CAAGAAATATGTTGGTGCAG+TGG | + | chr1.1:5947768-5947787 | MS.gene33000:intron | 40.0% | |
| GAAGAGGAAAAAGCCGATAA+GGG | + | chr1.1:5947824-5947843 | MS.gene33000:CDS | 40.0% | |
| CTAATTACCTTGGAACCCGT+AGG | - | chr1.1:5947652-5947671 | None:intergenic | 45.0% | |
| GGAAGAGGAAAAAGCCGATA+AGG | + | chr1.1:5947823-5947842 | MS.gene33000:CDS | 45.0% | |
| TTTCCTCTTCCCTTCTTCGT+CGG | - | chr1.1:5947814-5947833 | None:intergenic | 45.0% | |
| ! | TCTTTGGAGGAGTGATCCTA+CGG | + | chr1.1:5947633-5947652 | MS.gene33000:CDS | 45.0% |
| ATGTGGACTCCGACGAAGAA+GGG | + | chr1.1:5947802-5947821 | MS.gene33000:CDS | 50.0% | |
| CAGTGGTTGTGTAACGCATG+TGG | + | chr1.1:5947785-5947804 | MS.gene33000:intron | 50.0% | |
| TGGCGAAAGCTCATCATCTG+AGG | + | chr1.1:5947874-5947893 | MS.gene33000:CDS | 50.0% | |
| ! | CTTTGGAGGAGTGATCCTAC+GGG | + | chr1.1:5947634-5947653 | MS.gene33000:CDS | 50.0% |
| ACTCCGACGAAGAAGGGAAG+AGG | + | chr1.1:5947808-5947827 | MS.gene33000:CDS | 55.0% | |
| CATGTGGACTCCGACGAAGA+AGG | + | chr1.1:5947801-5947820 | MS.gene33000:CDS | 55.0% | |
| GAGTGATCCTACGGGTTCCA+AGG | + | chr1.1:5947642-5947661 | MS.gene33000:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 5947568 | 5947913 | 5947568 | ID=MS.gene33000 |
| chr1.1 | mRNA | 5947568 | 5947913 | 5947568 | ID=MS.gene33000.t1;Parent=MS.gene33000 |
| chr1.1 | exon | 5947568 | 5947663 | 5947568 | ID=MS.gene33000.t1.exon1;Parent=MS.gene33000.t1 |
| chr1.1 | CDS | 5947568 | 5947663 | 5947568 | ID=cds.MS.gene33000.t1;Parent=MS.gene33000.t1 |
| chr1.1 | exon | 5947788 | 5947913 | 5947788 | ID=MS.gene33000.t1.exon2;Parent=MS.gene33000.t1 |
| chr1.1 | CDS | 5947788 | 5947913 | 5947788 | ID=cds.MS.gene33000.t1;Parent=MS.gene33000.t1 |
| Gene Sequence |
| Protein sequence |
