AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene33153

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene33153.t1 MTR_1g013180 94.539 531 26 2 1 529 152 681 0.0 996
MS.gene33153.t1 MTR_1g013170 64.943 522 102 13 1 467 1 496 0.0 575
MS.gene33153.t1 MTR_7g117705 59.594 542 119 12 1 524 1 460 0.0 552
MS.gene33153.t1 MTR_3g106220 53.917 434 154 13 3 413 6 416 2.06e-133 402
MS.gene33153.t1 MTR_2g450070 51.453 344 142 6 3 325 16 355 4.63e-97 308
MS.gene33153.t1 MTR_2g450070 51.453 344 142 6 3 325 16 355 9.21e-97 308
MS.gene33153.t1 MTR_4g121020 56.522 253 108 2 2 254 28 278 1.12e-86 281
MS.gene33153.t1 MTR_4g121020 56.522 253 108 2 2 254 28 278 1.25e-86 281
MS.gene33153.t1 MTR_2g034960 54.264 258 114 1 1 258 25 278 1.13e-84 274
MS.gene33153.t1 MTR_4g131600 47.516 322 158 5 1 322 16 326 1.65e-84 273
MS.gene33153.t1 MTR_1g032570 51.923 260 108 3 4 253 10 262 5.04e-82 268
MS.gene33153.t1 MTR_8g079940 56.048 248 103 3 3 248 23 266 1.11e-81 265
MS.gene33153.t1 MTR_3g086100 55.242 248 103 2 5 248 13 256 1.56e-81 265
MS.gene33153.t1 MTR_4g131580 49.186 307 146 5 5 310 17 314 1.69e-81 266
MS.gene33153.t1 MTR_4g131570 47.077 325 156 5 3 322 15 328 1.00e-79 261
MS.gene33153.t1 MTR_1g013160 86.486 148 20 0 67 214 1 148 2.96e-76 238
MS.gene33153.t1 MTR_3g102590 50.588 255 71 3 2 253 5 207 3.77e-67 217
MS.gene33153.t1 MTR_3g102590 50.417 240 66 2 2 240 5 192 3.33e-62 202
MS.gene33153.t1 MTR_3g102600 41.745 321 89 8 1 321 4 226 2.11e-58 194
MS.gene33153.t1 MTR_3g086100 53.968 189 79 2 64 248 1 185 9.19e-56 195
MS.gene33153.t1 MTR_1g032570 50.500 200 82 3 64 253 1 193 2.39e-55 195
MS.gene33153.t1 MTR_3g102600 44.813 241 71 3 2 242 5 183 5.81e-52 175
MS.gene33153.t1 MTR_4g021760 39.095 243 120 4 5 247 11 225 3.93e-50 174
MS.gene33153.t1 MTR_4g021790 38.800 250 125 5 2 251 7 228 3.97e-47 165
MS.gene33153.t1 MTR_4g021855 37.200 250 129 4 2 251 7 228 2.09e-45 162
MS.gene33153.t1 MTR_4g021845 36.402 239 124 3 2 240 7 217 1.59e-44 157
MS.gene33153.t1 MTR_0450s0040 38.075 239 127 3 9 247 8 225 2.75e-43 157
MS.gene33153.t1 MTR_3g102600 38.182 275 72 7 47 321 1 177 8.94e-42 148
MS.gene33153.t1 MTR_4g121020 47.953 171 87 2 84 254 6 174 3.91e-41 156
MS.gene33153.t1 MTR_8g105600 33.841 328 186 9 5 318 9 319 1.03e-39 152
MS.gene33153.t1 MTR_7g026400 37.273 220 120 3 32 250 14 216 2.70e-35 140
MS.gene33153.t1 MTR_6g045327 31.579 247 139 3 4 249 13 230 1.40e-32 129
MS.gene33153.t1 MTR_3g102600 39.286 196 57 3 47 242 1 134 3.82e-32 120
MS.gene33153.t1 MTR_5g055260 34.818 247 106 8 2 248 7 198 8.80e-31 120
MS.gene33153.t1 MTR_8g032710 29.588 267 164 5 8 254 20 282 1.24e-26 113
MS.gene33153.t1 MTR_4g064730 45.192 104 57 0 162 265 102 205 3.78e-22 97.4
MS.gene33153.t1 MTR_3g092780 34.532 139 86 2 9 143 56 193 4.49e-22 100
MS.gene33153.t1 MTR_3g092780 34.532 139 86 2 9 143 56 193 5.11e-22 100
MS.gene33153.t1 MTR_6g016850 35.433 127 79 2 3 127 7 132 2.15e-21 91.7
MS.gene33153.t1 MTR_3g092780 34.286 140 86 3 9 143 56 194 9.07e-21 96.3
MS.gene33153.t1 MTR_3g092780 34.286 140 86 3 9 143 56 194 1.02e-20 96.3
MS.gene33153.t1 MTR_7g118260 35.220 159 89 4 2 152 5 157 1.33e-20 95.9
MS.gene33153.t1 MTR_4g023980 29.240 171 94 3 47 217 31 174 6.59e-20 87.8
MS.gene33153.t1 MTR_8g024260 34.965 143 82 2 11 142 38 180 6.44e-19 90.5
MS.gene33153.t1 MTR_7g118260 36.496 137 76 3 2 130 5 138 7.34e-19 90.1
MS.gene33153.t1 MTR_8g093040 34.677 124 81 0 4 127 13 136 1.14e-18 86.7
MS.gene33153.t1 MTR_7g089010 62.712 59 22 0 190 248 90 148 4.95e-18 85.1
MS.gene33153.t1 MTR_1g067110 35.246 122 76 2 9 127 85 206 9.37e-18 87.0
MS.gene33153.t1 MTR_1g067110 35.246 122 76 2 9 127 85 206 9.45e-18 87.0
MS.gene33153.t1 MTR_3g037390 33.871 124 79 1 9 129 24 147 9.51e-18 86.7
MS.gene33153.t1 MTR_4g061360 35.246 122 76 1 9 127 89 210 3.18e-17 85.5
MS.gene33153.t1 MTR_4g108880 32.258 124 81 1 9 129 33 156 2.82e-16 82.4
MS.gene33153.t1 MTR_5g014040 52.239 67 32 0 189 255 309 375 3.57e-15 78.6
MS.gene33153.t1 MTR_5g014040 33.835 133 84 2 5 136 14 143 1.53e-11 67.0
MS.gene33153.t1 MTR_2g090675 44.318 88 48 1 188 275 158 244 1.53e-14 76.3
MS.gene33153.t1 MTR_4g098870 57.895 57 24 0 189 245 308 364 2.64e-14 75.9
MS.gene33153.t1 MTR_4g098870 57.895 57 24 0 189 245 308 364 2.93e-14 75.9
MS.gene33153.t1 MTR_4g098870 57.895 57 24 0 189 245 347 403 3.06e-14 75.9
MS.gene33153.t1 MTR_4g098870 57.895 57 24 0 189 245 328 384 3.18e-14 75.9
MS.gene33153.t1 MTR_4g098870 57.895 57 24 0 189 245 328 384 3.18e-14 75.9
MS.gene33153.t1 MTR_4g078810 53.333 60 28 0 188 247 155 214 4.29e-14 74.7
MS.gene33153.t1 MTR_5g014040 48.611 72 32 1 189 255 269 340 3.11e-13 72.4
MS.gene33153.t1 MTR_5g014040 48.611 72 32 1 189 255 309 380 3.39e-13 72.4
MS.gene33153.t1 MTR_5g014040 33.835 133 84 2 5 136 14 143 1.63e-11 67.0
MS.gene33153.t1 MTR_6g453220 51.765 85 38 2 207 288 379 463 3.46e-13 72.4
MS.gene33153.t1 MTR_4g106590 32.824 131 75 2 9 126 26 156 3.26e-12 66.2
MS.gene33153.t1 MTR_1g080330 55.932 59 25 1 186 243 170 228 4.34e-12 67.4
MS.gene33153.t1 MTR_7g490310 32.374 139 73 3 11 128 11 149 5.00e-12 66.2
MS.gene33153.t1 MTR_8g038620 31.159 138 75 3 11 128 11 148 6.12e-12 64.7
MS.gene33153.t1 MTR_1g080330 55.932 59 25 1 186 243 170 228 6.80e-12 67.8
MS.gene33153.t1 MTR_1g093080 42.466 73 41 1 177 248 197 269 8.01e-12 67.4
MS.gene33153.t1 MTR_7g115530 58.929 56 22 1 189 243 216 271 1.18e-11 66.2
MS.gene33153.t1 MTR_7g115530 58.929 56 22 1 189 243 216 271 1.86e-11 65.9
MS.gene33153.t1 MTR_7g115530 58.929 56 22 1 189 243 216 271 2.15e-11 65.9
MS.gene33153.t1 MTR_1g053835 53.333 60 27 1 186 244 253 312 3.73e-11 65.5
MS.gene33153.t1 MTR_7g115530 58.929 56 22 1 189 243 216 271 4.08e-11 65.5
MS.gene33153.t1 MTR_6g088950 26.590 173 119 3 7 178 5 170 4.68e-11 62.8
MS.gene33153.t1 MTR_5g017980 45.902 61 33 0 188 248 189 249 7.64e-11 63.9
MS.gene33153.t1 MTR_5g017980 45.902 61 33 0 188 248 190 250 8.05e-11 63.9
MS.gene33153.t1 MTR_4g086835 47.458 59 31 0 190 248 273 331 9.70e-11 64.3
MS.gene33153.t1 MTR_4g113140 41.892 74 42 1 183 255 227 300 9.91e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene33153.t1 AT2G25180 59.677 310 116 5 3 309 11 314 1.50e-107 333
MS.gene33153.t1 AT4G31920 51.630 368 140 9 3 356 11 354 7.25e-103 320
MS.gene33153.t1 AT5G58080 47.912 407 146 14 3 387 12 374 8.60e-89 286
MS.gene33153.t1 AT3G16857 50.883 283 103 5 3 258 31 304 6.53e-84 274
MS.gene33153.t1 AT3G16857 50.177 283 105 4 3 258 31 304 2.10e-83 273
MS.gene33153.t1 AT1G67710 53.386 251 102 5 6 248 8 251 8.65e-73 241
MS.gene33153.t1 AT2G01760 48.134 268 131 3 1 261 3 269 1.15e-70 231
MS.gene33153.t1 AT3G16857 50.000 242 85 5 44 258 2 234 2.88e-66 226
MS.gene33153.t1 AT2G01760 43.289 298 133 4 1 263 3 299 1.24e-64 216
MS.gene33153.t1 AT5G49240 41.322 242 129 4 5 240 38 272 5.23e-51 176
MS.gene33153.t1 AT1G67710 51.295 193 79 5 64 248 1 186 4.83e-47 170
MS.gene33153.t1 AT3G62670 38.800 250 136 5 10 254 42 279 4.44e-44 161
MS.gene33153.t1 AT2G01760 45.366 205 104 3 64 261 1 204 3.16e-43 156
MS.gene33153.t1 AT1G49190 31.183 279 167 5 4 268 29 296 6.77e-37 141
MS.gene33153.t1 AT1G67710 46.821 173 77 5 84 248 12 177 2.80e-33 132
MS.gene33153.t1 AT5G07210 29.282 362 201 9 7 326 14 362 7.11e-33 133
MS.gene33153.t1 AT2G27070 30.000 300 150 7 7 260 14 299 6.56e-31 127
MS.gene33153.t1 AT5G07210 28.649 370 201 10 7 326 14 370 2.26e-30 125
MS.gene33153.t1 AT2G46790 39.024 123 70 2 9 127 37 158 1.70e-22 100
MS.gene33153.t1 AT2G46790 39.024 123 70 2 9 127 37 158 1.95e-22 100
MS.gene33153.t1 AT1G49190 38.400 125 77 0 4 128 29 153 5.41e-22 100
MS.gene33153.t1 AT5G59570 45.918 98 51 1 170 265 120 217 1.76e-20 92.4
MS.gene33153.t1 AT5G59570 45.918 98 51 1 170 265 120 217 1.76e-20 92.4
MS.gene33153.t1 AT5G24470 36.719 128 76 2 7 130 48 174 2.24e-20 95.1
MS.gene33153.t1 AT3G46640 47.778 90 45 1 178 265 129 218 4.02e-20 92.0
MS.gene33153.t1 AT3G46640 47.778 90 45 1 178 265 129 218 4.02e-20 92.0
MS.gene33153.t1 AT3G46640 47.778 90 45 1 178 265 129 218 4.97e-20 91.7
MS.gene33153.t1 AT2G40970 50.649 77 38 0 172 248 86 162 3.54e-19 87.4
MS.gene33153.t1 AT1G68210 26.894 264 162 6 9 243 13 274 3.57e-19 91.7
MS.gene33153.t1 AT3G10760 41.463 123 69 2 157 276 68 190 5.56e-19 88.6
MS.gene33153.t1 AT5G60100 37.008 127 76 2 5 127 59 185 7.72e-19 89.4
MS.gene33153.t1 AT5G60100 37.008 127 76 2 5 127 59 185 7.72e-19 89.4
MS.gene33153.t1 AT5G60100 37.008 127 76 2 5 127 59 185 7.72e-19 89.4
MS.gene33153.t1 AT5G60100 37.008 127 76 2 5 127 59 185 9.04e-19 89.7
MS.gene33153.t1 AT5G60100 37.008 127 76 2 5 127 59 185 9.04e-19 89.7
MS.gene33153.t1 AT5G60100 37.008 127 76 2 5 127 59 185 9.04e-19 89.7
MS.gene33153.t1 AT5G05090 54.286 70 32 0 179 248 69 138 1.59e-18 86.3
MS.gene33153.t1 AT5G60100 37.008 127 76 2 5 127 59 185 2.01e-18 89.0
MS.gene33153.t1 AT5G61380 33.871 124 79 1 9 129 19 142 7.25e-18 87.4
MS.gene33153.t1 AT5G02810 35.246 122 76 1 9 127 78 199 7.77e-18 87.4
MS.gene33153.t1 AT5G44190 56.923 65 28 0 183 247 139 203 1.01e-16 82.4
MS.gene33153.t1 AT5G44190 56.923 65 28 0 183 247 139 203 1.02e-16 82.0
MS.gene33153.t1 AT2G20570 40.367 109 57 2 165 273 130 230 6.90e-15 77.4
MS.gene33153.t1 AT2G20570 46.914 81 38 1 193 273 171 246 6.94e-14 74.3
MS.gene33153.t1 AT4G18020 54.098 61 26 1 187 247 294 352 2.41e-12 69.7
MS.gene33153.t1 AT4G18020 54.098 61 26 1 187 247 294 352 2.41e-12 69.7
MS.gene33153.t1 AT4G18020 54.098 61 26 1 187 247 294 352 2.41e-12 69.7
MS.gene33153.t1 AT4G18020 54.098 61 26 1 187 247 294 352 2.41e-12 69.7
MS.gene33153.t1 AT4G18020 54.098 61 26 1 187 247 294 352 2.41e-12 69.7
MS.gene33153.t1 AT4G18020 54.098 61 26 1 187 247 294 352 2.41e-12 69.7
MS.gene33153.t1 AT4G18020 54.098 61 26 1 187 247 294 352 2.56e-12 69.7
MS.gene33153.t1 AT4G18020 54.098 61 26 1 187 247 294 352 2.56e-12 69.7
MS.gene33153.t1 AT4G18020 54.098 61 26 1 187 247 294 352 2.56e-12 69.7
MS.gene33153.t1 AT4G18020 54.098 61 26 1 187 247 294 352 2.56e-12 69.7
MS.gene33153.t1 AT4G18020 54.098 61 26 1 187 247 294 352 2.56e-12 69.7
MS.gene33153.t1 AT3G57040 28.395 162 94 2 11 150 11 172 8.79e-12 65.5
MS.gene33153.t1 AT3G57040 28.395 162 94 2 11 150 11 172 8.79e-12 65.5
MS.gene33153.t1 AT1G68670 37.624 101 59 2 174 270 198 298 1.66e-11 66.2
MS.gene33153.t1 AT1G25550 43.038 79 44 1 182 259 200 278 3.64e-11 65.1
MS.gene33153.t1 AT3G25790 34.375 128 72 4 195 321 200 316 5.34e-11 64.7
MS.gene33153.t1 AT3G56380 31.298 131 76 2 9 125 20 150 5.64e-11 61.6
MS.gene33153.t1 AT1G10470 31.579 133 79 2 11 131 36 168 7.59e-11 63.2
MS.gene33153.t1 AT1G10470 31.579 133 79 2 11 131 22 154 7.63e-11 63.2

Find 113 sgRNAs with CRISPR-Local

Find 174 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAGAACATGTCTCAAGATTT+TGG 0.179210 1.3:-8640973 MS.gene33153:CDS
GGAAGGTAAGAGTTTGTTTC+TGG 0.198817 1.3:+8638389 None:intergenic
AGCTAATATGGCCGAAGATT+TGG 0.235811 1.3:-8638783 MS.gene33153:CDS
TTTAAGCTTCTTGAGCTTAT+TGG 0.242147 1.3:-8640198 MS.gene33153:CDS
ATTCCTTTCATCACCATCTT+TGG 0.253553 1.3:+8640036 None:intergenic
ACTCGACAGGTGATCATCTT+AGG 0.278334 1.3:-8638559 MS.gene33153:CDS
TGATGTCCCACCAGAGAAAT+TGG 0.298503 1.3:+8637838 None:intergenic
AATTTCCATCATGGTTGTTA+TGG 0.300876 1.3:+8638252 None:intergenic
TGTACCTCCTTCAATGTAAC+AGG 0.302320 1.3:+8639982 None:intergenic
CTTGCTGCTGTTAAACAATT+AGG 0.302670 1.3:-8639700 MS.gene33153:CDS
ATGAATGATCAATTTCCTAT+TGG 0.313965 1.3:-8641023 MS.gene33153:CDS
CCATTTCCACTATCAGAATT+AGG 0.318838 1.3:+8639898 None:intergenic
CCTAATTCTGATAGTGGAAA+TGG 0.330629 1.3:-8639898 MS.gene33153:CDS
GCTAATATGGCCGAAGATTT+GGG 0.348373 1.3:-8638782 MS.gene33153:CDS
CATGTGGTTGGTAGTTCAAT+AGG 0.353197 1.3:-8638218 MS.gene33153:CDS
GCATGGACAAAACCTTCTTT+TGG 0.354006 1.3:+8638368 None:intergenic
TGAAGGAGGTACAAATGATT+TGG 0.363320 1.3:-8639972 MS.gene33153:CDS
TCTAATAATGTGCCATTTCA+AGG 0.366783 1.3:-8638287 MS.gene33153:CDS
TTCACAACATGAATCATAGA+AGG 0.379234 1.3:+8638194 None:intergenic
TAGCCACATTCTCCCTTGTA+AGG 0.388290 1.3:+8639275 None:intergenic
GATATAACCCATTCTTGAAT+AGG 0.403697 1.3:+8638742 None:intergenic
AAAGAAGGTGCAATGACATT+TGG 0.412055 1.3:-8638433 MS.gene33153:CDS
CAATTATATGCCAATTTCTC+TGG 0.412361 1.3:-8637848 MS.gene33153:CDS
CTACTACTCATACCACTAGA+TGG 0.416526 1.3:+8638641 None:intergenic
AGAAGAAGCCTCGAGTTGTT+TGG 0.418038 1.3:-8639747 MS.gene33153:CDS
TACCATGGTATTGACACCTT+TGG 0.419217 1.3:+8640940 None:intergenic
TTATTATTATGAAACTGTCT+AGG 0.422621 1.3:+8638683 None:intergenic
TATGAACATTGCTGATTACT+AGG 0.423694 1.3:+8640238 None:intergenic
TTATTATCCCATCCTTGAAA+TGG 0.424148 1.3:+8638275 None:intergenic
AGCTTAAACCCATCCATGTC+TGG 0.428879 1.3:+8640213 None:intergenic
AACTCTTACCTTCCAAAAGA+AGG 0.429300 1.3:-8638380 MS.gene33153:CDS
GTTTCTGGTAATTGCAGCAT+AGG 0.430843 1.3:+8638404 None:intergenic
TGATAGATCCTATTCAAGAA+TGG 0.441988 1.3:-8638750 MS.gene33153:CDS
GATAGATCCTATTCAAGAAT+GGG 0.444292 1.3:-8638749 MS.gene33153:CDS
TTCAGATCCTTTCCATCTAG+TGG 0.445066 1.3:-8638653 MS.gene33153:CDS
GCAAGAACACGCATTCCAAT+AGG 0.451965 1.3:+8641008 None:intergenic
TCTTTGAACATCATTCAGAT+TGG 0.469109 1.3:+8638514 None:intergenic
ATGAATAATCAGATGAAGGC+TGG 0.470466 1.3:-8638080 MS.gene33153:CDS
ATGGGTTATATCAGTGGAGT+TGG 0.473556 1.3:-8638731 MS.gene33153:CDS
GGGAAACATTCGGCTGGGAC+AGG 0.477461 1.3:-8639877 MS.gene33153:CDS
GCTGAAACCTGTTACATTGA+AGG 0.477526 1.3:-8639989 MS.gene33153:CDS
CTAATTCTGATAGTGGAAAT+GGG 0.482228 1.3:-8639897 MS.gene33153:CDS
TGAGCTTATTGGACTTGAGA+TGG 0.484301 1.3:-8640187 MS.gene33153:CDS
TTTCTGGTAATTGCAGCATA+GGG 0.484334 1.3:+8638405 None:intergenic
AGAATCATTACCCAAATCTT+CGG 0.485778 1.3:+8638772 None:intergenic
TGTGAGTTCATGTTATATGT+AGG 0.486197 1.3:+8638332 None:intergenic
GAATGCTATTGAAGGAAACT+TGG 0.492011 1.3:-8638174 MS.gene33153:CDS
TGATTTGGCAACATGTAGTC+AGG 0.492304 1.3:-8639957 MS.gene33153:CDS
TGATCATCTTAGGTTTCAAT+CGG 0.494786 1.3:-8638549 MS.gene33153:CDS
CATGTTGTGAATGCTATTGA+AGG 0.494924 1.3:-8638182 MS.gene33153:CDS
GATGCGCCTAATTCTGATAG+TGG 0.498616 1.3:-8639904 MS.gene33153:CDS
TGAGAGTCTACAGAAGGAAA+TGG 0.499941 1.3:+8638488 None:intergenic
GCCAGTAGTGTTAATCTGCA+TGG 0.509822 1.3:-8638605 MS.gene33153:CDS
TAGTGGAAATGGGAAACATT+CGG 0.510168 1.3:-8639887 MS.gene33153:CDS
GGTTATATCAGTGGAGTTGG+TGG 0.511432 1.3:-8638728 MS.gene33153:CDS
GGAAATGGGAAACATTCGGC+TGG 0.517134 1.3:-8639883 MS.gene33153:CDS
TACCATCCTTCTCATGTGGT+TGG 0.521062 1.3:-8638230 MS.gene33153:CDS
GAAGAACGAAGATGATGATG+AGG 0.524981 1.3:-8639812 MS.gene33153:CDS
TTGCATAACTTACTTATGAC+AGG 0.526242 1.3:+8640159 None:intergenic
TCCATGCAGATTAACACTAC+TGG 0.528713 1.3:+8638604 None:intergenic
AAGGATGGTAATTTCCATCA+TGG 0.529123 1.3:+8638243 None:intergenic
AATAACCATAACAACCATGA+TGG 0.531658 1.3:-8638257 MS.gene33153:CDS
ATTCAGATTGGCGCGAGCTA+TGG 0.531857 1.3:+8638526 None:intergenic
TATTATTATGAAACTGTCTA+GGG 0.535599 1.3:+8638684 None:intergenic
TTCATATGCCAGACATGGAT+GGG 0.536690 1.3:-8640221 MS.gene33153:CDS
TATGATGAATAATCAGATGA+AGG 0.541034 1.3:-8638084 MS.gene33153:CDS
GAATGTGGCTAGCCATCTCC+AGG 0.545926 1.3:-8639263 MS.gene33153:intron
GTTCATATGCCAGACATGGA+TGG 0.546140 1.3:-8640222 MS.gene33153:CDS
ACCTCATGTGGTTTCAATGA+CGG 0.547065 1.3:+8638110 None:intergenic
TAATGTGCCATTTCAAGGAT+GGG 0.550140 1.3:-8638282 MS.gene33153:CDS
TTATATGCCAATTTCTCTGG+TGG 0.550617 1.3:-8637845 MS.gene33153:CDS
GAGTTGTTCAAATTATGTGC+AGG 0.552747 1.3:+8638578 None:intergenic
CATAATTTGAACAACTCGAC+AGG 0.552801 1.3:-8638572 MS.gene33153:CDS
TGGATGATGAAAACCTTACA+AGG 0.553288 1.3:-8639288 MS.gene33153:CDS
CAATGTTCATATGCCAGACA+TGG 0.556391 1.3:-8640226 MS.gene33153:CDS
ATAATGTGCCATTTCAAGGA+TGG 0.556574 1.3:-8638283 MS.gene33153:CDS
GGAAGAAGAAAACTAGCAAA+CGG 0.556828 1.3:-8639936 MS.gene33153:CDS
TTCATCATATATACCTCATG+TGG 0.558721 1.3:+8638098 None:intergenic
TGATGATGAGGATGAGAATG+AGG 0.567738 1.3:-8639800 MS.gene33153:CDS
CATGAGAGTCATGATCAAAC+CGG 0.571164 1.3:-8638015 MS.gene33153:intron
TATATGCCAATTTCTCTGGT+GGG 0.573019 1.3:-8637844 MS.gene33153:CDS
GAGTTCATGTTATATGTAGG+AGG 0.588472 1.3:+8638335 None:intergenic
ACTCATACCACTAGATGGAA+AGG 0.588967 1.3:+8638646 None:intergenic
GAACTACCAACCACATGAGA+AGG 0.589560 1.3:+8638224 None:intergenic
TGTTAGTGCACTTTGAGCAG+TGG 0.591132 1.3:+8640299 None:intergenic
AAACCTTACAAGGGAGAATG+TGG 0.595886 1.3:-8639278 MS.gene33153:CDS
TCAAGAATGGGTTATATCAG+TGG 0.601175 1.3:-8638737 MS.gene33153:CDS
TGCAGATTAACACTACTGGC+AGG 0.606997 1.3:+8638608 None:intergenic
GTGATGAAAGGAATTGATCA+TGG 0.607173 1.3:-8640027 MS.gene33153:CDS
CAATTATTGTGATCGTATCG+CGG 0.612466 1.3:-8638147 MS.gene33153:CDS
TGCGAATGATGATCCAAAGA+TGG 0.615837 1.3:-8640049 MS.gene33153:CDS
TGCATATTCTAATCTTGCCG+CGG 0.619450 1.3:+8637812 None:intergenic
GAAATGGGAAACATTCGGCT+GGG 0.620073 1.3:-8639882 MS.gene33153:CDS
TAGGAGGCATAGCAGCAGCA+TGG 0.623883 1.3:+8638351 None:intergenic
GAATGTTGAGAGTCTACAGA+AGG 0.629708 1.3:+8638482 None:intergenic
AAATTACCATCCTTCTCATG+TGG 0.631597 1.3:-8638234 MS.gene33153:CDS
GAAGCCTCGAGTTGTTTGGT+CGG 0.632069 1.3:-8639743 MS.gene33153:CDS
ACATGTTCTATCATCATCGA+CGG 0.636994 1.3:+8640986 None:intergenic
GATCCAAAGATGGTGATGAA+AGG 0.637771 1.3:-8640039 MS.gene33153:CDS
TCACAACATGAATCATAGAA+GGG 0.643072 1.3:+8638195 None:intergenic
TACCAACCACATGAGAAGGA+TGG 0.647791 1.3:+8638228 None:intergenic
CTCCAAAGGTGTCAATACCA+TGG 0.650196 1.3:-8640942 MS.gene33153:intron
AATCAAGCTCATTAGACATG+TGG 0.650925 1.3:+8638051 None:intergenic
TAACAGCAGCAAGAAACTTG+CGG 0.653136 1.3:+8639710 None:intergenic
TAATGAGCTTGATTCGCTCG+AGG 0.661321 1.3:-8638042 MS.gene33153:CDS
ACATATAACATGAACTCACA+AGG 0.670292 1.3:-8638329 MS.gene33153:CDS
CTGGTGGGACATCAATGCCG+CGG 0.675642 1.3:-8637829 MS.gene33153:CDS
GGATGATGAAAACCTTACAA+GGG 0.676423 1.3:-8639287 MS.gene33153:CDS
TCGTGACAATGATGACTCGA+CGG 0.681057 1.3:-8639773 MS.gene33153:CDS
GAAACCTGTTACATTGAAGG+AGG 0.684192 1.3:-8639986 MS.gene33153:CDS
TCAGCCGACCAAACAACTCG+AGG 0.687224 1.3:+8639739 None:intergenic
AACTGTCTAGGGTCATTCAG+TGG 0.690957 1.3:+8638695 None:intergenic
TCCGTCATTGAAACCACATG+AGG 0.701079 1.3:-8638111 MS.gene33153:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATAAGAAATCTAATCAT+TGG + chr1.3:8638443-8638462 None:intergenic 15.0%
!! AATATAAGATCACAAAATAT+TGG + chr1.3:8638344-8638363 None:intergenic 15.0%
!! ATTTAAGGAAAATACTTATA+TGG + chr1.3:8639895-8639914 None:intergenic 15.0%
!! TAAATTAAATGTAATCACAT+CGG - chr1.3:8639910-8639929 MS.gene33153:CDS 15.0%
!!! AGTTTATAAGCATTTTATTT+AGG - chr1.3:8639978-8639997 MS.gene33153:CDS 15.0%
!!! ATGTGATTACATTTAATTTA+AGG + chr1.3:8639910-8639929 None:intergenic 15.0%
!!! ATTTTTCATAATTTTTGTTG+TGG - chr1.3:8638505-8638524 MS.gene33153:CDS 15.0%
!!! TGTGATCTTATATTATTTTT+TGG - chr1.3:8638350-8638369 MS.gene33153:CDS 15.0%
!!! TTTTGTTCTTTTGATTTTTT+TGG - chr1.3:8637964-8637983 MS.gene33153:intron 15.0%
!! CATGCATAGAAAATTATTTA+GGG - chr1.3:8638737-8638756 MS.gene33153:CDS 20.0%
!! GATTCATAAAAATTCAACAT+TGG + chr1.3:8638178-8638197 None:intergenic 20.0%
!! TATAAGATAAGAAAAATACC+TGG + chr1.3:8639601-8639620 None:intergenic 20.0%
!! TATTATTATGAAACTGTCTA+GGG + chr1.3:8640162-8640181 None:intergenic 20.0%
!! TTATTATTATGAAACTGTCT+AGG + chr1.3:8640163-8640182 None:intergenic 20.0%
!!! AATCTCTAAAAGAATAAGAT+CGG + chr1.3:8638272-8638291 None:intergenic 20.0%
!!! AATTTTCTTTGATACTGTTT+AGG - chr1.3:8638214-8638233 MS.gene33153:CDS 20.0%
!!! ATTTCATAGATTTTATGATG+TGG - chr1.3:8638472-8638491 MS.gene33153:CDS 20.0%
!!! CATAGCATAAATTTTATTAC+CGG + chr1.3:8640850-8640869 None:intergenic 20.0%
! AATAAGATCGGTCAAAATAT+AGG + chr1.3:8638260-8638279 None:intergenic 25.0%
! ACTCGAATATCTTGATTAAA+TGG + chr1.3:8639261-8639280 None:intergenic 25.0%
! ATCCTTTACTTGAAAAAAGA+AGG - chr1.3:8640395-8640414 MS.gene33153:intron 25.0%
! ATGAATGATCAATTTCCTAT+TGG - chr1.3:8637820-8637839 MS.gene33153:CDS 25.0%
! CAAAAAAAACAAACATACCA+TGG + chr1.3:8637921-8637940 None:intergenic 25.0%
! CAGAAAGCAATATAGAAAAA+TGG + chr1.3:8640950-8640969 None:intergenic 25.0%
! CCATGCATAGAAAATTATTT+AGG - chr1.3:8638736-8638755 MS.gene33153:CDS 25.0%
! CTCGAATATCTTGATTAAAT+GGG + chr1.3:8639260-8639279 None:intergenic 25.0%
! GTTTAATAATCTTCATAGCA+TGG + chr1.3:8639342-8639361 None:intergenic 25.0%
! TATAGAAAAACTTCATAGCA+TGG + chr1.3:8639464-8639483 None:intergenic 25.0%
! TATGATGAATAATCAGATGA+AGG - chr1.3:8640759-8640778 MS.gene33153:intron 25.0%
! TCTGAAAAAACAAGCTAATA+TGG - chr1.3:8640048-8640067 MS.gene33153:CDS 25.0%
! TTAAATGGGAACAAATAAAG+AGG + chr1.3:8639246-8639265 None:intergenic 25.0%
! TTTAATCAAGATATTCGAGT+AGG - chr1.3:8639261-8639280 MS.gene33153:intron 25.0%
! TTTAATTGCATCCAAATTGT+TGG - chr1.3:8638383-8638402 MS.gene33153:CDS 25.0%
!! AAAAAAGATTCTTGACTTGA+TGG - chr1.3:8639535-8639554 MS.gene33153:intron 25.0%
!! CCTAAATAATTTTCTATGCA+TGG + chr1.3:8638739-8638758 None:intergenic 25.0%
!!! ATCAAGTCAAGAATCTTTTT+TGG + chr1.3:8639536-8639555 None:intergenic 25.0%
AAAGCCATCTAGAAAAAGAA+AGG - chr1.3:8639007-8639026 MS.gene33153:intron 30.0%
AATAACCATAACAACCATGA+TGG - chr1.3:8640586-8640605 MS.gene33153:intron 30.0%
AATTTCCATCATGGTTGTTA+TGG + chr1.3:8640594-8640613 None:intergenic 30.0%
ACATATAACATGAACTCACA+AGG - chr1.3:8640514-8640533 MS.gene33153:intron 30.0%
ACGCTCACATATTTCAAAAA+AGG - chr1.3:8639769-8639788 MS.gene33153:CDS 30.0%
AGAATCATTACCCAAATCTT+CGG + chr1.3:8640074-8640093 None:intergenic 30.0%
ATAAGAAAAATACCTGGAGA+TGG + chr1.3:8639595-8639614 None:intergenic 30.0%
ATCTAGTGAATCCAACAATT+TGG + chr1.3:8638397-8638416 None:intergenic 30.0%
CAATTATATGCCAATTTCTC+TGG - chr1.3:8640995-8641014 MS.gene33153:CDS 30.0%
GATAGATCCTATTCAAGAAT+GGG - chr1.3:8640094-8640113 MS.gene33153:intron 30.0%
GATATAACCCATTCTTGAAT+AGG + chr1.3:8640104-8640123 None:intergenic 30.0%
GGATCATAAGTTAAATGCAT+CGG + chr1.3:8640929-8640948 None:intergenic 30.0%
TCACAACATGAATCATAGAA+GGG + chr1.3:8640651-8640670 None:intergenic 30.0%
TCTTTGAACATCATTCAGAT+TGG + chr1.3:8640332-8640351 None:intergenic 30.0%
TGATAGATCCTATTCAAGAA+TGG - chr1.3:8640093-8640112 MS.gene33153:intron 30.0%
TGATCATCTTAGGTTTCAAT+CGG - chr1.3:8640294-8640313 MS.gene33153:CDS 30.0%
TGTGAGTTCATGTTATATGT+AGG + chr1.3:8640514-8640533 None:intergenic 30.0%
TTAAACGTTGATATTCGAGT+AGG - chr1.3:8639372-8639391 MS.gene33153:intron 30.0%
TTAGATGATCACAACCATTT+CGG - chr1.3:8639732-8639751 MS.gene33153:CDS 30.0%
TTATTATCCCATCCTTGAAA+TGG + chr1.3:8640571-8640590 None:intergenic 30.0%
TTCACAACATGAATCATAGA+AGG + chr1.3:8640652-8640671 None:intergenic 30.0%
TTCATCATATATACCTCATG+TGG + chr1.3:8640748-8640767 None:intergenic 30.0%
TTGCATAACTTACTTATGAC+AGG + chr1.3:8638687-8638706 None:intergenic 30.0%
TTTAAGCTTCTTGAGCTTAT+TGG - chr1.3:8638645-8638664 MS.gene33153:CDS 30.0%
TTTAGAGATTGCACATGATT+TGG - chr1.3:8638282-8638301 MS.gene33153:CDS 30.0%
! ATGATTTGAGCACATCATTT+TGG + chr1.3:8639867-8639886 None:intergenic 30.0%
! TAGAACATGTCTCAAGATTT+TGG - chr1.3:8637870-8637889 MS.gene33153:intron 30.0%
! TATGAACATTGCTGATTACT+AGG + chr1.3:8638608-8638627 None:intergenic 30.0%
! TTCTCCTTTCTTTTTCTAGA+TGG + chr1.3:8639014-8639033 None:intergenic 30.0%
!! CTAATTCTGATAGTGGAAAT+GGG - chr1.3:8638946-8638965 MS.gene33153:intron 30.0%
!! TCTAATAATGTGCCATTTCA+AGG - chr1.3:8640556-8640575 MS.gene33153:intron 30.0%
!!! CACCTTCTTTTTTCAAGTAA+AGG + chr1.3:8640400-8640419 None:intergenic 30.0%
!!! TCTTTTGATTTTTTTGGCCT+AGG - chr1.3:8637970-8637989 MS.gene33153:intron 30.0%
AAATTACCATCCTTCTCATG+TGG - chr1.3:8640609-8640628 MS.gene33153:intron 35.0%
AACCATTTCGGCATAAAATC+TGG - chr1.3:8639744-8639763 MS.gene33153:CDS 35.0%
AACTCTTACCTTCCAAAAGA+AGG - chr1.3:8640463-8640482 MS.gene33153:intron 35.0%
AAGGATGGTAATTTCCATCA+TGG + chr1.3:8640603-8640622 None:intergenic 35.0%
AATCAAGCTCATTAGACATG+TGG + chr1.3:8640795-8640814 None:intergenic 35.0%
ACATGTTCTATCATCATCGA+CGG + chr1.3:8637860-8637879 None:intergenic 35.0%
ACCATTTCGGCATAAAATCT+GGG - chr1.3:8639745-8639764 MS.gene33153:CDS 35.0%
ATGAATAATCAGATGAAGGC+TGG - chr1.3:8640763-8640782 MS.gene33153:intron 35.0%
ATTCCTTTCATCACCATCTT+TGG + chr1.3:8638810-8638829 None:intergenic 35.0%
CAATTATTGTGATCGTATCG+CGG - chr1.3:8640696-8640715 MS.gene33153:intron 35.0%
CATAATTTGAACAACTCGAC+AGG - chr1.3:8640271-8640290 MS.gene33153:CDS 35.0%
CATGTTGTGAATGCTATTGA+AGG - chr1.3:8640661-8640680 MS.gene33153:intron 35.0%
CCATTTCCACTATCAGAATT+AGG + chr1.3:8638948-8638967 None:intergenic 35.0%
CTTGCTGCTGTTAAACAATT+AGG - chr1.3:8639143-8639162 MS.gene33153:intron 35.0%
GAATGCTATTGAAGGAAACT+TGG - chr1.3:8640669-8640688 MS.gene33153:intron 35.0%
GAGTTCATGTTATATGTAGG+AGG + chr1.3:8640511-8640530 None:intergenic 35.0%
GAGTTGTTCAAATTATGTGC+AGG + chr1.3:8640268-8640287 None:intergenic 35.0%
GGAAGAAGAAAACTAGCAAA+CGG - chr1.3:8638907-8638926 MS.gene33153:intron 35.0%
GGATGATGAAAACCTTACAA+GGG - chr1.3:8639556-8639575 MS.gene33153:intron 35.0%
GTCATGTTTGGTATCTTTGT+CGG + chr1.3:8639653-8639672 None:intergenic 35.0%
GTGATGAAAGGAATTGATCA+TGG - chr1.3:8638816-8638835 MS.gene33153:intron 35.0%
TAGTGGAAATGGGAAACATT+CGG - chr1.3:8638956-8638975 MS.gene33153:intron 35.0%
TATATGCCAATTTCTCTGGT+GGG - chr1.3:8640999-8641018 MS.gene33153:CDS 35.0%
TCATTCAAATTACAGTGCCT+AGG + chr1.3:8637990-8638009 None:intergenic 35.0%
TGATGTGTTAGTGTCATGTT+TGG + chr1.3:8639665-8639684 None:intergenic 35.0%
TGGATGATGAAAACCTTACA+AGG - chr1.3:8639555-8639574 MS.gene33153:intron 35.0%
TTATATGCCAATTTCTCTGG+TGG - chr1.3:8640998-8641017 MS.gene33153:CDS 35.0%
! AAACGTTGAGATTAGTCCTA+GGG - chr1.3:8639295-8639314 MS.gene33153:CDS 35.0%
! AAAGAAGGTGCAATGACATT+TGG - chr1.3:8640410-8640429 MS.gene33153:intron 35.0%
! TAAACGTTGAGATTAGTCCT+AGG - chr1.3:8639294-8639313 MS.gene33153:CDS 35.0%
! TGAAGGAGGTACAAATGATT+TGG - chr1.3:8638871-8638890 MS.gene33153:intron 35.0%
! TTTCTGGTAATTGCAGCATA+GGG + chr1.3:8640441-8640460 None:intergenic 35.0%
!! ATAATGTGCCATTTCAAGGA+TGG - chr1.3:8640560-8640579 MS.gene33153:intron 35.0%
!! CCTAATTCTGATAGTGGAAA+TGG - chr1.3:8638945-8638964 MS.gene33153:intron 35.0%
!! TAATGTGCCATTTCAAGGAT+GGG - chr1.3:8640561-8640580 MS.gene33153:intron 35.0%
!! TCAAGAATGGGTTATATCAG+TGG - chr1.3:8640106-8640125 MS.gene33153:intron 35.0%
!!! AGTTGTTATTTTGCTTGCAG+AGG - chr1.3:8639505-8639524 MS.gene33153:intron 35.0%
AAACCTTACAAGGGAGAATG+TGG - chr1.3:8639565-8639584 MS.gene33153:intron 40.0%
ACCTCATGTGGTTTCAATGA+CGG + chr1.3:8640736-8640755 None:intergenic 40.0%
ACTCATACCACTAGATGGAA+AGG + chr1.3:8640200-8640219 None:intergenic 40.0%
AGCTAATATGGCCGAAGATT+TGG - chr1.3:8640060-8640079 MS.gene33153:intron 40.0%
CAATGTTCATATGCCAGACA+TGG - chr1.3:8638617-8638636 MS.gene33153:CDS 40.0%
CATGAGAGTCATGATCAAAC+CGG - chr1.3:8640828-8640847 MS.gene33153:intron 40.0%
CTACTACTCATACCACTAGA+TGG + chr1.3:8640205-8640224 None:intergenic 40.0%
GAAACCTGTTACATTGAAGG+AGG - chr1.3:8638857-8638876 MS.gene33153:intron 40.0%
GAATGTTGAGAGTCTACAGA+AGG + chr1.3:8640364-8640383 None:intergenic 40.0%
GCTAATATGGCCGAAGATTT+GGG - chr1.3:8640061-8640080 MS.gene33153:intron 40.0%
GCTGAAACCTGTTACATTGA+AGG - chr1.3:8638854-8638873 MS.gene33153:intron 40.0%
TAACAGCAGCAAGAAACTTG+CGG + chr1.3:8639136-8639155 None:intergenic 40.0%
TCCATGCAGATTAACACTAC+TGG + chr1.3:8640242-8640261 None:intergenic 40.0%
TGAGAGTCTACAGAAGGAAA+TGG + chr1.3:8640358-8640377 None:intergenic 40.0%
TGAGCTTATTGGACTTGAGA+TGG - chr1.3:8638656-8638675 MS.gene33153:CDS 40.0%
TGCGAATGATGATCCAAAGA+TGG - chr1.3:8638794-8638813 MS.gene33153:CDS 40.0%
TGTACCTCCTTCAATGTAAC+AGG + chr1.3:8638864-8638883 None:intergenic 40.0%
TTCAGATCCTTTCCATCTAG+TGG - chr1.3:8640190-8640209 MS.gene33153:CDS 40.0%
TTCATATGCCAGACATGGAT+GGG - chr1.3:8638622-8638641 MS.gene33153:CDS 40.0%
! AACGTTGAGATTAGTCCTAG+GGG - chr1.3:8639296-8639315 MS.gene33153:CDS 40.0%
! ATGGGTTATATCAGTGGAGT+TGG - chr1.3:8640112-8640131 MS.gene33153:intron 40.0%
! GAAGAACGAAGATGATGATG+AGG - chr1.3:8639031-8639050 MS.gene33153:intron 40.0%
! GATCCAAAGATGGTGATGAA+AGG - chr1.3:8638804-8638823 MS.gene33153:CDS 40.0%
! GCATGGACAAAACCTTCTTT+TGG + chr1.3:8640478-8640497 None:intergenic 40.0%
! GGAAGGTAAGAGTTTGTTTC+TGG + chr1.3:8640457-8640476 None:intergenic 40.0%
! GGACAAAACCTTCTTTTGGA+AGG + chr1.3:8640474-8640493 None:intergenic 40.0%
! TGATGATGAGGATGAGAATG+AGG - chr1.3:8639043-8639062 MS.gene33153:intron 40.0%
! TGATTTGGCAACATGTAGTC+AGG - chr1.3:8638886-8638905 MS.gene33153:intron 40.0%
! TTTTGCGCATTGAAGATCGA+GGG + chr1.3:8639187-8639206 None:intergenic 40.0%
! TTTTTGCGCATTGAAGATCG+AGG + chr1.3:8639188-8639207 None:intergenic 40.0%
!! CATGTGGTTGGTAGTTCAAT+AGG - chr1.3:8640625-8640644 MS.gene33153:intron 40.0%
!! GTTTCTGGTAATTGCAGCAT+AGG + chr1.3:8640442-8640461 None:intergenic 40.0%
!! TACCATGGTATTGACACCTT+TGG + chr1.3:8637906-8637925 None:intergenic 40.0%
!! TTTTGGAGAGACTTCTCCAA+AGG - chr1.3:8637887-8637906 MS.gene33153:intron 40.0%
ACTCGACAGGTGATCATCTT+AGG - chr1.3:8640284-8640303 MS.gene33153:CDS 45.0%
AGCTTAAACCCATCCATGTC+TGG + chr1.3:8638633-8638652 None:intergenic 45.0%
ATTAGTCCTAGGGGTGAATG+TGG - chr1.3:8639305-8639324 MS.gene33153:CDS 45.0%
GAAATGGGAAACATTCGGCT+GGG - chr1.3:8638961-8638980 MS.gene33153:intron 45.0%
GAACTACCAACCACATGAGA+AGG + chr1.3:8640622-8640641 None:intergenic 45.0%
GATGCGCCTAATTCTGATAG+TGG - chr1.3:8638939-8638958 MS.gene33153:intron 45.0%
GCAAGAACACGCATTCCAAT+AGG + chr1.3:8637838-8637857 None:intergenic 45.0%
GCACGTACACTTCAGATTGA+AGG + chr1.3:8639953-8639972 None:intergenic 45.0%
GCCAGTAGTGTTAATCTGCA+TGG - chr1.3:8640238-8640257 MS.gene33153:CDS 45.0%
GGTTATATCAGTGGAGTTGG+TGG - chr1.3:8640115-8640134 MS.gene33153:intron 45.0%
GTTCATATGCCAGACATGGA+TGG - chr1.3:8638621-8638640 MS.gene33153:CDS 45.0%
TACCAACCACATGAGAAGGA+TGG + chr1.3:8640618-8640637 None:intergenic 45.0%
TACCATCCTTCTCATGTGGT+TGG - chr1.3:8640613-8640632 MS.gene33153:intron 45.0%
TAGCCACATTCTCCCTTGTA+AGG + chr1.3:8639571-8639590 None:intergenic 45.0%
TCCGTCATTGAAACCACATG+AGG - chr1.3:8640732-8640751 MS.gene33153:intron 45.0%
TCGTGACAATGATGACTCGA+CGG - chr1.3:8639070-8639089 MS.gene33153:intron 45.0%
TGATGTCCCACCAGAGAAAT+TGG + chr1.3:8641008-8641027 None:intergenic 45.0%
TGCAGATTAACACTACTGGC+AGG + chr1.3:8640238-8640257 None:intergenic 45.0%
TGTTAGTGCACTTTGAGCAG+TGG + chr1.3:8638547-8638566 None:intergenic 45.0%
! AACTGTCTAGGGTCATTCAG+TGG + chr1.3:8640151-8640170 None:intergenic 45.0%
! AGAAGAAGCCTCGAGTTGTT+TGG - chr1.3:8639096-8639115 MS.gene33153:intron 45.0%
! GCCCAGATTTTATGCCGAAA+TGG + chr1.3:8639749-8639768 None:intergenic 45.0%
! TAATGAGCTTGATTCGCTCG+AGG - chr1.3:8640801-8640820 MS.gene33153:intron 45.0%
!! CTCCAAAGGTGTCAATACCA+TGG - chr1.3:8637901-8637920 MS.gene33153:intron 45.0%
!!! TTTTTTGAAATATTTAAAAT+AGG - chr1.3:8637934-8637953 MS.gene33153:intron 5.0%
ACATAGCCACATTCACCCCT+AGG + chr1.3:8639314-8639333 None:intergenic 50.0%
ATTCAGATTGGCGCGAGCTA+TGG + chr1.3:8640320-8640339 None:intergenic 50.0%
GGAAATGGGAAACATTCGGC+TGG - chr1.3:8638960-8638979 MS.gene33153:intron 50.0%
! GAAGCCTCGAGTTGTTTGGT+CGG - chr1.3:8639100-8639119 MS.gene33153:intron 50.0%
TCAGCCGACCAAACAACTCG+AGG + chr1.3:8639107-8639126 None:intergenic 55.0%
! GAATGTGGCTAGCCATCTCC+AGG - chr1.3:8639580-8639599 MS.gene33153:intron 55.0%
! TAGGAGGCATAGCAGCAGCA+TGG + chr1.3:8640495-8640514 None:intergenic 55.0%
CTGGTGGGACATCAATGCCG+CGG - chr1.3:8641014-8641033 MS.gene33153:CDS 60.0%
GGGAAACATTCGGCTGGGAC+AGG - chr1.3:8638966-8638985 MS.gene33153:intron 60.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 8637820 8641045 8637820 ID=MS.gene33153
chr1.3 mRNA 8637820 8641045 8637820 ID=MS.gene33153.t1;Parent=MS.gene33153
chr1.3 exon 8640943 8641045 8640943 ID=MS.gene33153.t1.exon1;Parent=MS.gene33153.t1
chr1.3 CDS 8640943 8641045 8640943 ID=cds.MS.gene33153.t1;Parent=MS.gene33153.t1
chr1.3 exon 8640172 8640324 8640172 ID=MS.gene33153.t1.exon2;Parent=MS.gene33153.t1
chr1.3 CDS 8640172 8640324 8640172 ID=cds.MS.gene33153.t1;Parent=MS.gene33153.t1
chr1.3 exon 8639692 8640078 8639692 ID=MS.gene33153.t1.exon3;Parent=MS.gene33153.t1
chr1.3 CDS 8639692 8640078 8639692 ID=cds.MS.gene33153.t1;Parent=MS.gene33153.t1
chr1.3 exon 8639264 8639340 8639264 ID=MS.gene33153.t1.exon4;Parent=MS.gene33153.t1
chr1.3 CDS 8639264 8639340 8639264 ID=cds.MS.gene33153.t1;Parent=MS.gene33153.t1
chr1.3 exon 8638016 8638831 8638016 ID=MS.gene33153.t1.exon5;Parent=MS.gene33153.t1
chr1.3 CDS 8638016 8638831 8638016 ID=cds.MS.gene33153.t1;Parent=MS.gene33153.t1
chr1.3 exon 8637820 8637873 8637820 ID=MS.gene33153.t1.exon6;Parent=MS.gene33153.t1
chr1.3 CDS 8637820 8637873 8637820 ID=cds.MS.gene33153.t1;Parent=MS.gene33153.t1
Gene Sequence

>MS.gene33153

ATGAATGATCAATTTCCTATTGGAATGCGTGTTCTTGCCGTCGATGATGATAGAACATGTCTCAAGATTTTGGAGAGACTTCTCCAAAGGTGTCAATACCATGGTATGTTTGTTTTTTTTGAAATATTTAAAATAGGTTTTTGTTTTTGTTCTTTTGATTTTTTTGGCCTAGGCACTGTAATTTGAATGATAACATTAACAGTTACTTTTTTTGTAAACTTGAATTTGTCAAATTGATTGAGTTTTTTTTTCGTTTATTTTGTTTTTTGTTGTGACAAATTATCTATTTTGACAAATATGTGCATTTATGTATGTGTTAGATTATAGATTATAGATTATCATCAGTGAAGTTGAGCCAATGTTGAATTTTTATGAATCAAATAGATTAGTTTTAAATTTTCTTTGATACTGTTTAGGCTCAACTAAGTTATTTTTGACCTATATTTTGACCGATCTTATTCTTTTAGAGATTGCACATGATTTGGTTTTTAAAGTTCTAAATTTTTAAAAAATTCTTGTGTCCAATATTTTGTGATCTTATATTATTTTTTGGATTTCAAAAATTTAATTGCATCCAAATTGTTGGATTCACTAGATGCAGATTGTCATGCAATTCTTGTCCAATGATTAGATTTCTTATTTTTTAATTAACATTTCATAGATTTTATGATGTGGTTGTATCTGAATTTTTCATAATTTTTGTTGTGGTTATTTGTTGCAGTAACCACTGCTCAAAGTGCACTAACAGCACTGAACTTGTTGAGAGAAAATAAAAGCAATTTTGACCTAGTAATCAGCAATGTTCATATGCCAGACATGGATGGGTTTAAGCTTCTTGAGCTTATTGGACTTGAGATGGATTTACCTGTCATAAGTAAGTTATGCAATTCTTCAATCTTATGTTATTGTTATTGATCCATGCATAGAAAATTATTTAGGGTTTTGATAAAAAACTGTTTGATTTCAGTGTTTTCTGCGAATGATGATCCAAAGATGGTGATGAAAGGAATTGATCATGGTGCTTGTGATTATCTGCTGAAACCTGTTACATTGAAGGAGGTACAAATGATTTGGCAACATGTAGTCAGGAAGAAGAAAACTAGCAAACGGAGCAATCTTGATGCGCCTAATTCTGATAGTGGAAATGGGAAACATTCGGCTGGGACAGGCAATTCTGATCAAATTGAAAAGCCATCTAGAAAAAGAAAGGAGAAGAACGAAGATGATGATGAGGATGAGAATGAGGATGATCGTGACAATGATGACTCGACGGCACAGAAGAAGCCTCGAGTTGTTTGGTCGGCTGACTTACACCGCAAGTTTCTTGCTGCTGTTAAACAATTAGGCTTTGAAAGTATGTTGTTCCCTCGATCTTCAATGCGCAAAAACAGTTGTCATCATAACTTTTATCAATTGCTATATTCCTCTTTATTTGTTCCCATTTAATCAAGATATTCGAGTAGGTTATAGAAATTAAACGTTGAGATTAGTCCTAGGGGTGAATGTGGCTATGTTTTAGCCATGCTATGAAGATTATTAAACGTTGAGATTATTAAACGTTGATATTCGAGTAGGTTATAGAAATTAAACGTTGAAATTAAACGTCAAGATTATTAATCAAGATCATTACTTATGTTTTAGCCATGCTATGAAGTTTTTCTATACATAACTGTGTTTCTCATTATAGTTGTTATTTTGCTTGCAGAGGCTGTGCCAAAAAAGATTCTTGACTTGATGGATGATGAAAACCTTACAAGGGAGAATGTGGCTAGCCATCTCCAGGTATTTTTCTTATCTTATATTTAGCGTTAGAAACTATGAATCACAACACCGACAAAGATACCAAACATGACACTAACACATCAACACTGCTAATAATTTAAAAACTTAAAATAATTAAATTAAGAGAGTCTTAGATGATCACAACCATTTCGGCATAAAATCTGGGCACGCTCACATATTTCAAAAAAGGTAGAGAGAAAAAATAATTAAATAATGTTTATCTTGATAATAATTAAATGATATGTATTTTGATGCGTGTGCGCCAAAATGATGTGCTCAAATCATTGTGACCATATAAGTATTTTCCTTAAATTAAATGTAATCACATCGGCATCAGAAACTAGACAACCTTCAATCTGAAGTGTACGTGCTACGTAGTTTATAAGCATTTTATTTAGGAAAACTAACTACTTTTATTTGAAATTTCTGCAGAAATACAGACTCTATCTGAAAAAACAAGCTAATATGGCCGAAGATTTGGGTAATGATTCTGATAGATCCTATTCAAGAATGGGTTATATCAGTGGAGTTGGTGGACATGTGCAGCCACTGAATGACCCTAGACAGTTTCATAATAATAACAGTGCTTTCAGATCCTTTCCATCTAGTGGTATGAGTAGTAGATTGTACACTCCTGCCAGTAGTGTTAATCTGCATGGATTTCCTGCACATAATTTGAACAACTCGACAGGTGATCATCTTAGGTTTCAATCGGCCATAGCTCGCGCCAATCTGAATGATGTTCAAAGAATGCCATTTCCTTCTGTAGACTCTCAACATTCACAGTTTTCTCATCCTTTACTTGAAAAAAGAAGGTGCAATGACATTTGGTCAAGCCCTATGCTGCAATTACCAGAAACAAACTCTTACCTTCCAAAAGAAGGTTTTGTCCATGCTGCTGCTATGCCTCCTACATATAACATGAACTCACAAGGTATTATTTTTAACAGCAGTTCTAATAATGTGCCATTTCAAGGATGGGATAATAACCATAACAACCATGATGGAAATTACCATCCTTCTCATGTGGTTGGTAGTTCAATAGGCCCTTCTATGATTCATGTTGTGAATGCTATTGAAGGAAACTTGGACAACAATTATTGTGATCGTATCGCGGAGTTACATGAAGTTCCGTCATTGAAACCACATGAGGTATATATGATGAATAATCAGATGAAGGCTGGAAATAGCCACATGTCTAATGAGCTTGATTCGCTCGAGGACGTCATGAGAGTCATGATCAAACCGGTAATAAAATTTATGCTATGCAATGAAGTTCTTATCTGAGTTTTTTTGTTTGATTGTTGATTTGAACATAAAAGTTCCGATGCATTTAACTTATGATCCATTTTTCTATATTGCTTTCTGATCATTAATTCTTTTGATGCAGAAACAATTATATGCCAATTTCTCTGGTGGGACATCAATGCCGCGGCAAGATTAG
Protein sequence

>MS.gene33153.t1

MNDQFPIGMRVLAVDDDRTCLKILERLLQRCQYHVTTAQSALTALNLLRENKSNFDLVISNVHMPDMDGFKLLELIGLEMDLPVIMFSANDDPKMVMKGIDHGACDYLLKPVTLKEVQMIWQHVVRKKKTSKRSNLDAPNSDSGNGKHSAGTGNSDQIEKPSRKRKEKNEDDDEDENEDDRDNDDSTAQKKPRVVWSADLHRKFLAAVKQLGFEKAVPKKILDLMDDENLTRENVASHLQKYRLYLKKQANMAEDLGNDSDRSYSRMGYISGVGGHVQPLNDPRQFHNNNSAFRSFPSSGMSSRLYTPASSVNLHGFPAHNLNNSTGDHLRFQSAIARANLNDVQRMPFPSVDSQHSQFSHPLLEKRRCNDIWSSPMLQLPETNSYLPKEGFVHAAAMPPTYNMNSQGIIFNSSSNNVPFQGWDNNHNNHDGNYHPSHVVGSSIGPSMIHVVNAIEGNLDNNYCDRIAELHEVPSLKPHEVYMMNNQMKAGNSHMSNELDSLEDVMRVMIKPKQLYANFSGGTSMPRQD