AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene33154

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene33154.t1 MTR_1g013170 92.986 499 33 2 1 499 1 497 0.0 902
MS.gene33154.t1 MTR_1g013180 65.134 522 104 12 1 498 152 619 0.0 597
MS.gene33154.t1 MTR_7g117705 58.487 489 102 13 1 487 1 390 9.69e-165 474
MS.gene33154.t1 MTR_3g106220 47.466 592 196 17 3 499 6 577 2.86e-152 449
MS.gene33154.t1 MTR_2g450070 44.266 497 222 14 3 454 16 502 1.76e-110 342
MS.gene33154.t1 MTR_2g450070 44.266 497 222 14 3 454 16 502 3.85e-110 342
MS.gene33154.t1 MTR_4g131600 50.147 341 149 7 1 338 16 338 4.38e-95 300
MS.gene33154.t1 MTR_4g121020 57.812 256 106 2 2 257 28 281 4.20e-93 297
MS.gene33154.t1 MTR_4g121020 57.812 256 106 2 2 257 28 281 5.13e-93 297
MS.gene33154.t1 MTR_2g034960 51.536 293 131 3 1 289 25 310 1.90e-92 293
MS.gene33154.t1 MTR_4g131580 47.527 364 171 8 5 361 17 367 6.36e-92 292
MS.gene33154.t1 MTR_4g131570 47.917 336 159 7 3 335 15 337 4.85e-91 290
MS.gene33154.t1 MTR_1g032570 54.135 266 105 3 4 259 10 268 2.28e-90 288
MS.gene33154.t1 MTR_8g079940 56.589 258 102 3 3 256 23 274 1.16e-85 274
MS.gene33154.t1 MTR_1g013160 93.919 148 9 0 67 214 1 148 4.53e-83 254
MS.gene33154.t1 MTR_3g086100 55.823 249 102 2 5 249 13 257 6.99e-83 268
MS.gene33154.t1 MTR_3g102590 52.239 268 71 4 2 266 5 218 6.39e-75 236
MS.gene33154.t1 MTR_3g102590 54.583 240 56 2 2 240 5 192 1.21e-69 221
MS.gene33154.t1 MTR_1g032570 53.398 206 79 3 64 259 1 199 2.95e-63 215
MS.gene33154.t1 MTR_3g102600 41.640 317 85 7 1 317 4 220 1.01e-60 199
MS.gene33154.t1 MTR_3g086100 54.211 190 79 2 64 249 1 186 9.52e-57 197
MS.gene33154.t1 MTR_4g021760 39.326 267 132 4 5 271 11 247 1.63e-54 186
MS.gene33154.t1 MTR_3g102600 46.473 241 67 4 2 242 5 183 2.94e-54 181
MS.gene33154.t1 MTR_4g021855 38.462 247 124 3 2 248 7 225 1.27e-48 169
MS.gene33154.t1 MTR_4g021790 39.676 247 121 4 2 248 7 225 1.42e-48 169
MS.gene33154.t1 MTR_4g021845 38.494 239 119 4 2 240 7 217 6.06e-47 162
MS.gene33154.t1 MTR_4g121020 50.000 174 85 2 84 257 6 177 1.93e-46 170
MS.gene33154.t1 MTR_0450s0040 38.174 241 128 3 9 249 8 227 7.74e-44 158
MS.gene33154.t1 MTR_3g102600 36.900 271 71 7 47 317 1 171 3.52e-41 146
MS.gene33154.t1 MTR_8g105600 36.101 277 150 6 5 274 9 265 4.07e-39 150
MS.gene33154.t1 MTR_7g026400 36.986 219 122 2 32 250 14 216 1.00e-37 146
MS.gene33154.t1 MTR_6g045327 34.286 245 131 3 4 247 13 228 3.68e-37 141
MS.gene33154.t1 MTR_3g102600 67.857 84 27 0 47 130 1 84 1.58e-33 124
MS.gene33154.t1 MTR_3g102600 67.925 53 17 0 190 242 82 134 1.02e-18 83.2
MS.gene33154.t1 MTR_6g453220 80.899 89 17 0 207 295 379 467 2.61e-33 132
MS.gene33154.t1 MTR_5g055260 34.413 247 107 7 2 248 7 198 3.35e-31 121
MS.gene33154.t1 MTR_8g032710 30.078 256 155 5 8 243 20 271 9.30e-28 116
MS.gene33154.t1 MTR_4g064730 48.673 113 52 2 156 262 94 206 9.14e-24 102
MS.gene33154.t1 MTR_3g092780 34.532 139 86 2 9 143 56 193 1.08e-22 102
MS.gene33154.t1 MTR_3g092780 34.532 139 86 2 9 143 56 193 1.39e-22 102
MS.gene33154.t1 MTR_6g016850 37.008 127 77 2 3 127 7 132 5.33e-22 93.2
MS.gene33154.t1 MTR_3g092780 37.008 127 75 2 9 131 56 181 7.01e-22 99.8
MS.gene33154.t1 MTR_3g092780 37.008 127 75 2 9 131 56 181 9.08e-22 99.4
MS.gene33154.t1 MTR_4g023980 32.934 167 85 4 51 217 35 174 2.09e-21 92.0
MS.gene33154.t1 MTR_7g118260 32.258 186 111 5 2 178 5 184 3.36e-21 97.4
MS.gene33154.t1 MTR_8g024260 33.929 168 97 3 11 164 38 205 1.20e-20 95.9
MS.gene33154.t1 MTR_7g118260 35.766 137 77 3 2 130 5 138 6.04e-20 93.2
MS.gene33154.t1 MTR_7g089010 66.667 60 20 0 190 249 90 149 6.99e-20 90.5
MS.gene33154.t1 MTR_1g067110 36.885 122 74 2 9 127 85 206 1.58e-19 92.4
MS.gene33154.t1 MTR_1g067110 36.885 122 74 2 9 127 85 206 1.63e-19 92.4
MS.gene33154.t1 MTR_4g061360 36.220 127 77 2 5 127 84 210 7.33e-19 90.5
MS.gene33154.t1 MTR_3g037390 33.871 124 79 1 9 129 24 147 1.45e-18 89.0
MS.gene33154.t1 MTR_8g093040 35.484 124 80 0 4 127 13 136 2.44e-18 85.5
MS.gene33154.t1 MTR_4g108880 32.258 124 81 1 9 129 33 156 4.21e-17 84.7
MS.gene33154.t1 MTR_5g014040 56.061 66 29 0 184 249 304 369 4.54e-17 84.3
MS.gene33154.t1 MTR_5g014040 34.677 124 77 2 5 127 14 134 1.33e-11 67.4
MS.gene33154.t1 MTR_2g090675 39.474 114 58 2 188 301 158 260 8.37e-17 82.8
MS.gene33154.t1 MTR_4g078810 51.515 66 32 0 188 253 155 220 7.11e-16 80.1
MS.gene33154.t1 MTR_4g098870 57.377 61 26 0 189 249 308 368 1.79e-15 79.3
MS.gene33154.t1 MTR_4g098870 57.377 61 26 0 189 249 308 368 1.87e-15 79.3
MS.gene33154.t1 MTR_4g098870 57.377 61 26 0 189 249 328 388 2.05e-15 79.3
MS.gene33154.t1 MTR_4g098870 57.377 61 26 0 189 249 328 388 2.05e-15 79.3
MS.gene33154.t1 MTR_4g098870 57.377 61 26 0 189 249 347 407 2.14e-15 79.3
MS.gene33154.t1 MTR_5g014040 52.113 71 29 1 184 249 264 334 3.71e-15 78.2
MS.gene33154.t1 MTR_5g014040 52.113 71 29 1 184 249 304 374 4.35e-15 78.2
MS.gene33154.t1 MTR_5g014040 34.677 124 77 2 5 127 14 134 1.55e-11 67.0
MS.gene33154.t1 MTR_4g086835 39.091 110 46 2 186 295 269 357 1.45e-14 75.9
MS.gene33154.t1 MTR_1g093080 50.000 68 33 1 184 250 204 271 7.19e-14 73.6
MS.gene33154.t1 MTR_7g115530 50.000 76 37 1 189 263 216 291 7.71e-14 72.8
MS.gene33154.t1 MTR_7g115530 50.000 76 37 1 189 263 216 291 1.79e-13 72.0
MS.gene33154.t1 MTR_5g017980 51.613 62 30 0 188 249 189 250 2.11e-13 71.6
MS.gene33154.t1 MTR_5g017980 51.613 62 30 0 188 249 190 251 2.31e-13 71.6
MS.gene33154.t1 MTR_4g113140 48.684 76 37 2 176 250 221 295 2.47e-13 72.4
MS.gene33154.t1 MTR_7g115530 50.000 76 37 1 189 263 216 291 3.11e-13 72.0
MS.gene33154.t1 MTR_1g080330 60.345 58 22 1 187 243 171 228 4.00e-13 70.1
MS.gene33154.t1 MTR_7g115530 64.286 56 19 1 189 243 216 271 4.11e-13 70.9
MS.gene33154.t1 MTR_4g086835 38.739 111 46 3 186 295 269 358 4.67e-13 71.2
MS.gene33154.t1 MTR_1g080330 52.941 68 31 1 187 253 171 238 5.43e-13 70.9
MS.gene33154.t1 MTR_2g023580 45.238 84 45 1 190 272 54 137 1.86e-12 67.4
MS.gene33154.t1 MTR_7g098250 51.471 68 32 1 187 253 258 325 2.24e-12 69.7
MS.gene33154.t1 MTR_5g017980 50.794 63 30 1 188 249 190 252 2.55e-12 68.2
MS.gene33154.t1 MTR_6g032990 43.878 98 46 4 191 284 42 134 3.31e-12 68.6
MS.gene33154.t1 MTR_5g054300 49.231 65 32 1 185 248 192 256 3.63e-12 68.2
MS.gene33154.t1 MTR_6g088950 27.485 171 118 2 7 176 5 170 4.27e-12 65.9
MS.gene33154.t1 MTR_2g086450 56.364 55 23 1 191 244 45 99 5.09e-12 67.8
MS.gene33154.t1 MTR_2g027800 57.627 59 24 1 187 244 35 93 6.15e-12 67.0
MS.gene33154.t1 MTR_2g027800 57.627 59 24 1 187 244 35 93 6.40e-12 67.0
MS.gene33154.t1 MTR_1g090670 55.932 59 25 1 187 244 40 98 8.97e-12 66.2
MS.gene33154.t1 MTR_7g115530 62.963 54 19 1 189 241 216 269 1.10e-11 65.9
MS.gene33154.t1 MTR_5g027440 38.235 102 55 3 187 280 21 122 1.27e-11 65.9
MS.gene33154.t1 MTR_7g088070 45.783 83 41 3 191 270 50 131 1.63e-11 65.5
MS.gene33154.t1 MTR_1g053830 54.717 53 23 1 193 244 20 72 2.35e-11 60.8
MS.gene33154.t1 MTR_5g041350 52.727 55 25 1 191 244 15 69 2.96e-11 65.5
MS.gene33154.t1 MTR_8g038620 28.986 138 78 3 11 128 11 148 3.92e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene33154.t1 AT2G25180 58.333 336 122 9 3 332 11 334 6.58e-112 343
MS.gene33154.t1 AT4G31920 49.010 404 148 11 3 390 11 372 7.84e-104 322
MS.gene33154.t1 AT3G16857 49.673 306 120 5 3 285 31 325 8.33e-92 293
MS.gene33154.t1 AT3G16857 49.673 306 120 5 3 285 31 325 1.57e-91 293
MS.gene33154.t1 AT5G58080 47.258 383 156 11 3 367 12 366 3.16e-87 281
MS.gene33154.t1 AT2G01760 43.333 360 176 9 1 337 3 357 2.22e-78 250
MS.gene33154.t1 AT1G67710 54.980 251 100 4 6 249 8 252 9.05e-78 253
MS.gene33154.t1 AT3G16857 49.057 265 101 5 44 285 2 255 2.15e-74 246
MS.gene33154.t1 AT2G01760 40.206 388 176 10 1 337 3 385 3.67e-72 235
MS.gene33154.t1 AT1G67710 53.368 193 77 4 64 249 1 187 2.03e-51 181
MS.gene33154.t1 AT2G01760 40.067 297 150 9 64 337 1 292 2.20e-49 172
MS.gene33154.t1 AT5G49240 38.819 237 142 2 5 240 38 272 2.53e-49 171
MS.gene33154.t1 AT3G62670 37.795 254 139 7 10 257 42 282 9.87e-44 160
MS.gene33154.t1 AT1G67710 47.977 173 77 4 84 249 12 178 6.80e-37 142
MS.gene33154.t1 AT1G49190 31.752 274 169 5 4 267 29 294 1.73e-35 137
MS.gene33154.t1 AT5G07210 31.900 279 144 7 7 250 14 281 2.57e-34 136
MS.gene33154.t1 AT5G07210 27.681 401 217 14 7 353 14 395 6.86e-32 129
MS.gene33154.t1 AT2G27070 31.338 284 140 7 7 249 14 283 1.13e-31 128
MS.gene33154.t1 AT2G46790 39.837 123 69 2 9 127 37 158 4.18e-24 105
MS.gene33154.t1 AT2G46790 39.837 123 69 2 9 127 37 158 5.01e-24 105
MS.gene33154.t1 AT5G59570 50.538 93 46 0 170 262 120 212 3.99e-23 100
MS.gene33154.t1 AT5G59570 50.538 93 46 0 170 262 120 212 3.99e-23 100
MS.gene33154.t1 AT1G49190 37.600 125 78 0 4 128 29 153 1.53e-22 101
MS.gene33154.t1 AT5G24470 36.719 128 76 2 7 130 48 174 1.10e-21 98.6
MS.gene33154.t1 AT3G46640 37.736 159 85 4 104 249 45 202 2.27e-21 95.5
MS.gene33154.t1 AT3G46640 37.736 159 85 4 104 249 45 202 2.27e-21 95.5
MS.gene33154.t1 AT2G40970 46.296 108 54 1 169 272 79 186 2.37e-21 93.6
MS.gene33154.t1 AT3G46640 37.736 159 85 4 104 249 45 202 3.81e-21 94.7
MS.gene33154.t1 AT5G02810 38.525 122 72 1 9 127 78 199 1.36e-20 95.9
MS.gene33154.t1 AT5G60100 37.795 127 75 2 5 127 59 185 2.87e-20 93.2
MS.gene33154.t1 AT5G60100 37.795 127 75 2 5 127 59 185 2.87e-20 93.2
MS.gene33154.t1 AT5G60100 37.795 127 75 2 5 127 59 185 2.87e-20 93.2
MS.gene33154.t1 AT3G10760 66.667 60 20 0 190 249 104 163 3.01e-20 92.4
MS.gene33154.t1 AT5G05090 53.165 79 37 0 171 249 61 139 3.01e-20 90.9
MS.gene33154.t1 AT5G60100 37.795 127 75 2 5 127 59 185 4.97e-20 93.2
MS.gene33154.t1 AT5G60100 37.795 127 75 2 5 127 59 185 4.97e-20 93.2
MS.gene33154.t1 AT5G60100 37.795 127 75 2 5 127 59 185 4.97e-20 93.2
MS.gene33154.t1 AT5G60100 37.795 127 75 2 5 127 59 185 1.03e-19 92.4
MS.gene33154.t1 AT5G44190 57.746 71 30 0 183 253 139 209 1.77e-19 90.1
MS.gene33154.t1 AT5G44190 57.746 71 30 0 183 253 139 209 1.90e-19 90.5
MS.gene33154.t1 AT5G61380 33.871 124 79 1 9 129 19 142 6.53e-19 90.5
MS.gene33154.t1 AT2G20570 38.583 127 65 2 140 253 89 215 3.08e-17 84.3
MS.gene33154.t1 AT2G20570 62.069 58 22 0 196 253 174 231 1.12e-15 79.7
MS.gene33154.t1 AT4G18020 52.857 70 31 1 185 254 292 359 1.35e-14 76.6
MS.gene33154.t1 AT4G18020 52.857 70 31 1 185 254 292 359 1.35e-14 76.6
MS.gene33154.t1 AT4G18020 52.857 70 31 1 185 254 292 359 1.35e-14 76.6
MS.gene33154.t1 AT4G18020 52.857 70 31 1 185 254 292 359 1.35e-14 76.6
MS.gene33154.t1 AT4G18020 52.857 70 31 1 185 254 292 359 1.35e-14 76.6
MS.gene33154.t1 AT4G18020 52.857 70 31 1 185 254 292 359 1.35e-14 76.6
MS.gene33154.t1 AT4G18020 52.857 70 31 1 185 254 292 359 1.79e-14 76.3
MS.gene33154.t1 AT4G18020 52.857 70 31 1 185 254 292 359 1.79e-14 76.3
MS.gene33154.t1 AT4G18020 52.857 70 31 1 185 254 292 359 1.79e-14 76.3
MS.gene33154.t1 AT4G18020 52.857 70 31 1 185 254 292 359 1.79e-14 76.3
MS.gene33154.t1 AT4G18020 52.857 70 31 1 185 254 292 359 1.79e-14 76.3
MS.gene33154.t1 AT2G03500 54.839 62 27 1 190 250 232 293 4.63e-13 71.6
MS.gene33154.t1 AT3G57040 28.395 162 94 2 11 150 11 172 2.23e-12 67.4
MS.gene33154.t1 AT3G57040 28.395 162 94 2 11 150 11 172 2.23e-12 67.4
MS.gene33154.t1 AT5G45580 53.731 67 28 2 181 244 11 77 2.43e-12 67.8
MS.gene33154.t1 AT5G45580 55.932 59 25 1 187 244 3 61 4.16e-12 67.0
MS.gene33154.t1 AT3G04030 56.364 55 23 1 191 244 45 99 4.16e-12 68.2
MS.gene33154.t1 AT3G04030 56.364 55 23 1 191 244 45 99 4.94e-12 68.2
MS.gene33154.t1 AT5G18240 58.182 55 22 1 191 244 45 99 6.88e-12 67.8
MS.gene33154.t1 AT5G18240 58.182 55 22 1 191 244 45 99 6.88e-12 67.8
MS.gene33154.t1 AT5G18240 58.182 55 22 1 191 244 45 99 7.38e-12 67.4
MS.gene33154.t1 AT5G18240 58.182 55 22 1 191 244 45 99 7.93e-12 67.4
MS.gene33154.t1 AT5G18240 58.182 55 22 1 191 244 45 99 7.93e-12 67.4
MS.gene33154.t1 AT3G56380 31.298 131 76 2 9 125 20 150 1.00e-11 63.5
MS.gene33154.t1 AT4G13640 41.837 98 40 3 187 273 33 124 1.79e-11 65.5
MS.gene33154.t1 AT4G13640 41.837 98 40 3 187 273 33 124 1.92e-11 65.5
MS.gene33154.t1 AT2G40670 32.331 133 74 2 9 125 29 161 2.41e-11 62.8
MS.gene33154.t1 AT1G25550 51.667 60 28 1 190 248 208 267 2.63e-11 65.5
MS.gene33154.t1 AT3G56380 31.538 130 76 2 9 125 20 149 3.33e-11 62.0
MS.gene33154.t1 AT1G49560 47.222 72 37 1 186 256 187 258 5.79e-11 64.3
MS.gene33154.t1 AT3G24120 41.111 90 42 2 187 265 37 126 6.81e-11 63.9
MS.gene33154.t1 AT3G24120 41.111 90 42 2 187 265 37 126 6.87e-11 63.9
MS.gene33154.t1 AT1G68670 42.857 77 43 1 173 248 197 273 7.12e-11 64.3
MS.gene33154.t1 AT4G37180 49.180 61 30 1 189 248 208 268 7.74e-11 64.3
MS.gene33154.t1 AT2G40670 32.576 132 74 2 9 125 29 160 8.21e-11 61.2
MS.gene33154.t1 AT4G37180 49.180 61 30 1 189 248 215 275 8.32e-11 63.9

Find 107 sgRNAs with CRISPR-Local

Find 155 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAGAACATGTCTCAAGATTT+TGG 0.179210 1.3:-8651628 MS.gene33154:CDS
TTGTTTGTGAAACTGAAATT+TGG 0.235409 1.3:+8649459 None:intergenic
GGAGGTTATTGGTCATGTTT+TGG 0.244923 1.3:+8649481 None:intergenic
ACTGAAATTTGGGAGGTTAT+TGG 0.250594 1.3:+8649470 None:intergenic
ATTCCTTTCATCACCATCTT+TGG 0.256348 1.3:+8650786 None:intergenic
ATCTAAAAGGATTGTTATAA+TGG 0.273251 1.3:+8649706 None:intergenic
CATTTGGATTGCCGCGAGTT+AGG 0.293700 1.3:+8649535 None:intergenic
ACAGAAGGTGCAATGGTATT+TGG 0.304024 1.3:-8649338 MS.gene33154:CDS
TGTACCTCTTTCAGTCTAAC+AGG 0.317233 1.3:+8650732 None:intergenic
CCATTTCCACTATCAGAATT+AGG 0.318838 1.3:+8650648 None:intergenic
ATGAATGATCAGTTTCCTAT+TGG 0.330551 1.3:-8651678 MS.gene33154:CDS
CCTAATTCTGATAGTGGAAA+TGG 0.330629 1.3:-8650648 MS.gene33154:CDS
ATACCTCTTTGAACATCATT+TGG 0.333499 1.3:+8649519 None:intergenic
CCAGATGGAAAGGATCTAAA+AGG 0.348535 1.3:+8649693 None:intergenic
TCCAACTCCACTTCTTAAAT+AGG 0.361147 1.3:+8649770 None:intergenic
GGAAGATAAGAGTTTGTTCC+AGG 0.362766 1.3:+8649294 None:intergenic
ATAGGCTCTTCTATGATTCC+TGG 0.370372 1.3:-8649162 MS.gene33154:CDS
GGAAACTTGGACTACAATTA+TGG 0.374368 1.3:-8649117 MS.gene33154:CDS
TGTTTGTGAAACTGAAATTT+GGG 0.375065 1.3:+8649460 None:intergenic
AACAATCCCTTGATGTTAGA+AGG 0.383955 1.3:-8649441 MS.gene33154:CDS
TTTAAGCTCCTTGAGCTTGT+TGG 0.387237 1.3:-8650941 MS.gene33154:CDS
ACATGGTGCGTGTGATTATC+TGG 0.387305 1.3:-8650760 MS.gene33154:CDS
ATTTGGATTGCCGCGAGTTA+GGG 0.388130 1.3:+8649536 None:intergenic
GTTGCTGCGGTTAATCAATT+AGG 0.395251 1.3:-8650450 MS.gene33154:CDS
TGAAAGAGGTACAGATCATT+TGG 0.404224 1.3:-8650722 MS.gene33154:CDS
ATGCCTCATGTGTCATTTCA+AGG 0.407109 1.3:-8649246 MS.gene33154:CDS
AATGTGATCGGCAGTTCAAT+AGG 0.412930 1.3:-8649180 MS.gene33154:CDS
TATGAACATTGCTGATTACT+AGG 0.423694 1.3:+8650981 None:intergenic
TCATGTGTCATTTCAAGGTT+GGG 0.431069 1.3:-8649241 MS.gene33154:CDS
TACCATGGTATTGACACCTT+TGG 0.431203 1.3:+8651595 None:intergenic
CCATTCATATTCACATTAGT+AGG 0.431732 1.3:+8649642 None:intergenic
GGACATTTGCATACATTGAA+TGG 0.439183 1.3:-8649747 MS.gene33154:CDS
CCTACTAATGTGAATATGAA+TGG 0.451724 1.3:-8649642 MS.gene33154:CDS
TCATTTGGCAGCATGTAATC+AGG 0.452747 1.3:-8650707 MS.gene33154:CDS
GACGCGCCTAATTCTGATAG+TGG 0.455329 1.3:-8650654 MS.gene33154:CDS
AGAAGAAGCCTCGAACTGTT+TGG 0.455763 1.3:-8650497 MS.gene33154:CDS
CTGACTCGGCTGAAGAGTTC+CGG 0.455864 1.3:+8649608 None:intergenic
TGAGCTTGTTGGACTTGAGA+TGG 0.457732 1.3:-8650930 MS.gene33154:CDS
GCAAGAACTCGCATTCCAAT+AGG 0.458411 1.3:+8651663 None:intergenic
AGAATTTCCTTCTAACATCA+AGG 0.459500 1.3:+8649434 None:intergenic
GGACTACAATTATGGTGATT+CGG 0.460683 1.3:-8649109 MS.gene33154:CDS
ATCAGTTCAAGAATCTTCTT+CGG 0.461313 1.3:+8650168 None:intergenic
CTCATGTGTCATTTCAAGGT+TGG 0.464023 1.3:-8649242 MS.gene33154:CDS
AGCTTAAATCCATCCATGTC+TGG 0.471845 1.3:+8650956 None:intergenic
GCATGGTTAAAAGATTCTCT+TGG 0.472134 1.3:+8649273 None:intergenic
GGGAAACATTCGGCTGGGAC+AGG 0.477461 1.3:-8650627 MS.gene33154:CDS
CTAATTCTGATAGTGGAAAT+GGG 0.482228 1.3:-8650647 MS.gene33154:CDS
ATGAAAAGCCATCTAGAAAA+CGG 0.489677 1.3:-8650590 MS.gene33154:CDS
GATACATCCTATTTAAGAAG+TGG 0.493596 1.3:-8649777 MS.gene33154:CDS
TCCTATTTAAGAAGTGGAGT+TGG 0.497128 1.3:-8649771 MS.gene33154:CDS
GATAATAACAATAATCATGA+TGG 0.497207 1.3:-8649219 MS.gene33154:CDS
CACTGCTCAAAACGCGATAA+CGG 0.499559 1.3:-8651041 MS.gene33154:CDS
TTTAGATCCTTTCCATCTGG+TGG 0.500957 1.3:-8649690 MS.gene33154:CDS
TCCCTTTACTTGAAAACAGA+AGG 0.502275 1.3:-8649353 MS.gene33154:CDS
GGAAGACAAACAAGATCATA+TGG 0.510045 1.3:-8649823 MS.gene33154:CDS
TAGTGGAAATGGGAAACATT+CGG 0.510168 1.3:-8650637 MS.gene33154:CDS
AGCACAATGCAACAACTACC+TGG 0.515145 1.3:-8649312 MS.gene33154:CDS
GGAAATGGGAAACATTCGGC+TGG 0.517134 1.3:-8650633 MS.gene33154:CDS
TGCCGATCACATTAGAAGAA+TGG 0.518061 1.3:+8649190 None:intergenic
TACCATTCTTCTAATGTGAT+CGG 0.518616 1.3:-8649192 MS.gene33154:CDS
GAAGGTGGCCAGCCATCTCC+AGG 0.523004 1.3:-8650121 MS.gene33154:intron
TTGCATAACTTACTTATGAC+AGG 0.526242 1.3:+8650902 None:intergenic
TATTTAAGAAGTGGAGTTGG+AGG 0.527033 1.3:-8649768 MS.gene33154:CDS
TGCCGCGAGTTAGGGCCGAT+TGG 0.528037 1.3:+8649544 None:intergenic
ATGAATGGATTGCATTCTGC+CGG 0.538303 1.3:-8649627 MS.gene33154:CDS
GAAACCTGTTAGACTGAAAG+AGG 0.539886 1.3:-8650736 MS.gene33154:CDS
TGTCGAGCAAGAAGGAAACT+TGG 0.540152 1.3:-8649130 MS.gene33154:CDS
TATTGGAATGCGAGTTCTTG+CGG 0.540178 1.3:-8651661 MS.gene33154:CDS
TCAGCGGACCAAACAGTTCG+AGG 0.550196 1.3:+8650489 None:intergenic
AAGGATAAGAATGAAGATGA+TGG 0.554575 1.3:-8650567 MS.gene33154:CDS
CAATGTTCATATGCCAGACA+TGG 0.556391 1.3:-8650969 MS.gene33154:CDS
CTTGAAAACAGAAGGTGCAA+TGG 0.560167 1.3:-8649345 MS.gene33154:CDS
GAATTTCCTTCTAACATCAA+GGG 0.562420 1.3:+8649435 None:intergenic
ATGGTGATTCGGTACAATGA+AGG 0.563231 1.3:-8649098 None:intergenic
TAAGAATGAAGATGATGGTG+AGG 0.566326 1.3:-8650562 MS.gene33154:CDS
TGATCAGCTAAAGTTCCAAT+CGG 0.568120 1.3:-8649559 MS.gene33154:CDS
TGTATCTGAACCACTAGCTA+TGG 0.569493 1.3:+8649794 None:intergenic
ATGAATGTTGTCGAGCAAGA+AGG 0.574186 1.3:-8649138 MS.gene33154:CDS
TGATGGTGAGGAAGAAAGTG+AGG 0.580573 1.3:-8650550 MS.gene33154:CDS
CATAACACCACCAGATGGAA+AGG 0.581151 1.3:+8649683 None:intergenic
GTTCATATGCCAGACATGGA+TGG 0.584384 1.3:-8650965 MS.gene33154:CDS
TGCTCGACAACATTCATACC+AGG 0.593541 1.3:+8649144 None:intergenic
TTTAAATTATGTGTCTGACT+CGG 0.594323 1.3:+8649594 None:intergenic
TCTCAAGTCCAACAAGCTCA+AGG 0.597253 1.3:+8650933 None:intergenic
AATTCTCAAGACAAACAAGT+AGG 0.598098 1.3:-8649417 MS.gene33154:CDS
CAAGACAAACAAGTAGGTGA+AGG 0.598346 1.3:-8649411 MS.gene33154:CDS
TGAAAATCTGACAAGGGAGA+AGG 0.601495 1.3:-8650139 MS.gene33154:CDS
AATCCAAATGATGTTCAAAG+AGG 0.602398 1.3:-8649522 MS.gene33154:CDS
TTCCAATCGGCCCTAACTCG+CGG 0.602702 1.3:-8649546 MS.gene33154:CDS
AAATCTGACAAGGGAGAAGG+TGG 0.609003 1.3:-8650136 MS.gene33154:CDS
ACACCGCAAGTTTGTTGCTG+CGG 0.610830 1.3:-8650463 MS.gene33154:CDS
TGCGAATGATGATCCAAAGA+TGG 0.615837 1.3:-8650799 MS.gene33154:CDS
CTACTCATAACACCACCAGA+TGG 0.616853 1.3:+8649678 None:intergenic
GAAATGGGAAACATTCGGCT+GGG 0.620073 1.3:-8650632 MS.gene33154:CDS
AAAGCCATCTAGAAAACGGA+AGG 0.622229 1.3:-8650586 MS.gene33154:CDS
ATAACTTACTTATGACAGGT+AGG 0.629417 1.3:+8650906 None:intergenic
GATCCAAAGATGGTGATGAA+AGG 0.637771 1.3:-8650789 MS.gene33154:CDS
ACACATGAGGCATAGCAGCA+TGG 0.642777 1.3:+8649256 None:intergenic
CTCCAAAGGTGTCAATACCA+TGG 0.650196 1.3:-8651597 MS.gene33154:intron
ATGGCAGCAGCCATAGCTAG+TGG 0.659987 1.3:-8649804 MS.gene33154:CDS
TAACCGCAGCAACAAACTTG+CGG 0.661176 1.3:+8650460 None:intergenic
AAACTTGCGGTGTAAATCAG+CGG 0.665866 1.3:+8650473 None:intergenic
CAACCTTGAAATGACACATG+AGG 0.667024 1.3:+8649243 None:intergenic
TTGTGAAACTGAAATTTGGG+AGG 0.670570 1.3:+8649463 None:intergenic
TGAATGTTGAAAATCTGACA+AGG 0.702505 1.3:-8650146 MS.gene33154:CDS
GAATGTTGAAAATCTGACAA+GGG 0.715396 1.3:-8650145 MS.gene33154:CDS
GTGATGAAAGGAATTGAACA+TGG 0.742148 1.3:-8650777 MS.gene33154:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATGATATTTATTTTAGTTA+TGG - chr1.3:8649693-8649712 MS.gene33154:CDS 10.0%
!!! AATTTTATTTGATATTGTTT+AGG - chr1.3:8649471-8649490 MS.gene33154:CDS 10.0%
!!! ATTATTATTTTCAATAAAGT+TGG - chr1.3:8649369-8649388 MS.gene33154:CDS 10.0%
!!! TATTTTGAAATATTAAAGAT+GGG - chr1.3:8649246-8649265 MS.gene33154:CDS 10.0%
!!! TTATTTTGAAATATTAAAGA+TGG - chr1.3:8649245-8649264 MS.gene33154:CDS 10.0%
!! AAAAAACAAGATTAAAATCA+AGG + chr1.3:8649273-8649292 None:intergenic 15.0%
!! TAATTGAATTTAACAACATA+TGG - chr1.3:8650796-8650815 MS.gene33154:CDS 15.0%
!! TATCAATAATATAAGATTGA+AGG + chr1.3:8649905-8649924 None:intergenic 15.0%
!!! TTTTTAGTAAAGAATATTTG+AGG + chr1.3:8650510-8650529 None:intergenic 15.0%
!! AAATCTAATGATATATAGCT+AGG - chr1.3:8649301-8649320 MS.gene33154:CDS 20.0%
!! AATCTAATGATATATAGCTA+GGG - chr1.3:8649302-8649321 MS.gene33154:CDS 20.0%
!! ATCTAAAAGGATTGTTATAA+TGG + chr1.3:8651076-8651095 None:intergenic 20.0%
!! GATAATAACAATAATCATGA+TGG - chr1.3:8651560-8651579 MS.gene33154:intron 20.0%
!!! ATTTTGATAAACTGGAAAAA+AGG + chr1.3:8650444-8650463 None:intergenic 20.0%
!!! TAATTGATTTCTACAAGTAT+TGG + chr1.3:8649440-8649459 None:intergenic 20.0%
! CTGCAGAAAAATTAAAAAAC+AGG + chr1.3:8650901-8650920 None:intergenic 25.0%
! GAAGTCTTCAAATAATACAT+TGG + chr1.3:8650488-8650507 None:intergenic 25.0%
! TCTAAAAAGAATTAGTTGTG+CGG - chr1.3:8650935-8650954 MS.gene33154:CDS 25.0%
! TCTAGCATATGCATAAATAT+AGG + chr1.3:8649350-8649369 None:intergenic 25.0%
! TGTTTGTGAAACTGAAATTT+GGG + chr1.3:8651322-8651341 None:intergenic 25.0%
! TTGTTTGTGAAACTGAAATT+TGG + chr1.3:8651323-8651342 None:intergenic 25.0%
! TTTAAATTATGTGTCTGACT+CGG + chr1.3:8651188-8651207 None:intergenic 25.0%
!!! GCACAAATATTTTGATAAAC+TGG + chr1.3:8650452-8650471 None:intergenic 25.0%
AAGGATAAGAATGAAGATGA+TGG - chr1.3:8650212-8650231 MS.gene33154:intron 30.0%
AATCCAAATGATGTTCAAAG+AGG - chr1.3:8651257-8651276 MS.gene33154:intron 30.0%
AATTCTCAAGACAAACAAGT+AGG - chr1.3:8651362-8651381 MS.gene33154:intron 30.0%
ACATAAGATCACCAAAGATT+AGG + chr1.3:8649599-8649618 None:intergenic 30.0%
AGAATTTCCTTCTAACATCA+AGG + chr1.3:8651348-8651367 None:intergenic 30.0%
ATAACTTACTTATGACAGGT+AGG + chr1.3:8649876-8649895 None:intergenic 30.0%
ATACCTCTTTGAACATCATT+TGG + chr1.3:8651263-8651282 None:intergenic 30.0%
ATCAGTTCAAGAATCTTCTT+CGG + chr1.3:8650614-8650633 None:intergenic 30.0%
ATGAAAAGCCATCTAGAAAA+CGG - chr1.3:8650189-8650208 MS.gene33154:intron 30.0%
ATGAATGATCAGTTTCCTAT+TGG - chr1.3:8649101-8649120 MS.gene33154:CDS 30.0%
CCATTCATATTCACATTAGT+AGG + chr1.3:8651140-8651159 None:intergenic 30.0%
CCTACTAATGTGAATATGAA+TGG - chr1.3:8651137-8651156 MS.gene33154:intron 30.0%
GAATGTTGAAAATCTGACAA+GGG - chr1.3:8650634-8650653 MS.gene33154:CDS 30.0%
GAATTTCCTTCTAACATCAA+GGG + chr1.3:8651347-8651366 None:intergenic 30.0%
GATACATCCTATTTAAGAAG+TGG - chr1.3:8651002-8651021 MS.gene33154:CDS 30.0%
TACCATTCTTCTAATGTGAT+CGG - chr1.3:8651587-8651606 MS.gene33154:intron 30.0%
TGAATGTTGAAAATCTGACA+AGG - chr1.3:8650633-8650652 MS.gene33154:CDS 30.0%
TGAGAAAAACACTTCAGAAT+CGG + chr1.3:8650563-8650582 None:intergenic 30.0%
TTGCATAACTTACTTATGAC+AGG + chr1.3:8649880-8649899 None:intergenic 30.0%
! ATGATATATAGCTAGGGTTT+TGG - chr1.3:8649308-8649327 MS.gene33154:CDS 30.0%
! TAGAACATGTCTCAAGATTT+TGG - chr1.3:8649151-8649170 MS.gene33154:CDS 30.0%
! TATGAACATTGCTGATTACT+AGG + chr1.3:8649801-8649820 None:intergenic 30.0%
! TTGTTGCTTCTTTCAAAGAT+TGG - chr1.3:8649524-8649543 MS.gene33154:CDS 30.0%
!! AAGAGATAAGAAAAAGTACC+TGG + chr1.3:8650679-8650698 None:intergenic 30.0%
!! CTAATTCTGATAGTGGAAAT+GGG - chr1.3:8650132-8650151 MS.gene33154:CDS 30.0%
!! TTATTGGTCATGTTTTGGAT+AGG + chr1.3:8651296-8651315 None:intergenic 30.0%
!!! ACCTTCTGTTTTCAAGTAAA+GGG + chr1.3:8651430-8651449 None:intergenic 30.0%
AACAATCCCTTGATGTTAGA+AGG - chr1.3:8651338-8651357 MS.gene33154:intron 35.0%
AATCGGAAAAATTCGTAGCA+TGG + chr1.3:8650546-8650565 None:intergenic 35.0%
ACTGAAATTTGGGAGGTTAT+TGG + chr1.3:8651312-8651331 None:intergenic 35.0%
ATCTTTGTGTGCCTAATCTT+TGG - chr1.3:8649585-8649604 MS.gene33154:CDS 35.0%
ATTCCTTTCATCACCATCTT+TGG + chr1.3:8649996-8650015 None:intergenic 35.0%
CCATTTCCACTATCAGAATT+AGG + chr1.3:8650134-8650153 None:intergenic 35.0%
GCATGGTTAAAAGATTCTCT+TGG + chr1.3:8651509-8651528 None:intergenic 35.0%
GGAAACTTGGACTACAATTA+TGG - chr1.3:8651662-8651681 MS.gene33154:CDS 35.0%
GGAAGACAAACAAGATCATA+TGG - chr1.3:8650956-8650975 MS.gene33154:CDS 35.0%
GGACATTTGCATACATTGAA+TGG - chr1.3:8651032-8651051 MS.gene33154:CDS 35.0%
GGACTACAATTATGGTGATT+CGG - chr1.3:8651670-8651689 MS.gene33154:CDS 35.0%
GTGATGAAAGGAATTGAACA+TGG - chr1.3:8650002-8650021 MS.gene33154:intron 35.0%
TAGTGGAAATGGGAAACATT+CGG - chr1.3:8650142-8650161 MS.gene33154:CDS 35.0%
TATTTAAGAAGTGGAGTTGG+AGG - chr1.3:8651011-8651030 MS.gene33154:CDS 35.0%
TCCAACTCCACTTCTTAAAT+AGG + chr1.3:8651012-8651031 None:intergenic 35.0%
TCCCTTTACTTGAAAACAGA+AGG - chr1.3:8651426-8651445 MS.gene33154:intron 35.0%
TCCTATTTAAGAAGTGGAGT+TGG - chr1.3:8651008-8651027 MS.gene33154:CDS 35.0%
TGATAGTTGTTATCTTGCAG+AGG - chr1.3:8650583-8650602 MS.gene33154:CDS 35.0%
TGATCAGCTAAAGTTCCAAT+CGG - chr1.3:8651220-8651239 MS.gene33154:intron 35.0%
TTGTGAAACTGAAATTTGGG+AGG + chr1.3:8651319-8651338 None:intergenic 35.0%
! TAAGAATGAAGATGATGGTG+AGG - chr1.3:8650217-8650236 MS.gene33154:intron 35.0%
! TGAAAGAGGTACAGATCATT+TGG - chr1.3:8650057-8650076 MS.gene33154:intron 35.0%
! TTATCCTTCCGTTTTCTAGA+TGG + chr1.3:8650200-8650219 None:intergenic 35.0%
! TTTTGCGCATTGAAGAAAGA+GGG + chr1.3:8650373-8650392 None:intergenic 35.0%
! TTTTTGCGCATTGAAGAAAG+AGG + chr1.3:8650374-8650393 None:intergenic 35.0%
!! CCTAATTCTGATAGTGGAAA+TGG - chr1.3:8650131-8650150 MS.gene33154:CDS 35.0%
!! GGTGATCTTATGTTGTTTCT+TGG - chr1.3:8649606-8649625 MS.gene33154:CDS 35.0%
!! TAAGAAAAAGTACCTGGAGA+TGG + chr1.3:8650673-8650692 None:intergenic 35.0%
!! TCATGTGTCATTTCAAGGTT+GGG - chr1.3:8651538-8651557 MS.gene33154:intron 35.0%
!! TGATTTCTACAAGTATTGGC+TGG + chr1.3:8649436-8649455 None:intergenic 35.0%
!! TTTTGGAGAAACTTCTCCAA+AGG - chr1.3:8649168-8649187 MS.gene33154:CDS 35.0%
!!! CACCTTCTGTTTTCAAGTAA+AGG + chr1.3:8651431-8651450 None:intergenic 35.0%
AAACTTGCGGTGTAAATCAG+CGG + chr1.3:8650309-8650328 None:intergenic 40.0%
AAAGCCATCTAGAAAACGGA+AGG - chr1.3:8650193-8650212 MS.gene33154:intron 40.0%
AATGTGATCGGCAGTTCAAT+AGG - chr1.3:8651599-8651618 MS.gene33154:CDS 40.0%
AGCTTAAATCCATCCATGTC+TGG + chr1.3:8649826-8649845 None:intergenic 40.0%
ATAGGCTCTTCTATGATTCC+TGG - chr1.3:8651617-8651636 MS.gene33154:CDS 40.0%
ATGAATGTTGTCGAGCAAGA+AGG - chr1.3:8651641-8651660 MS.gene33154:CDS 40.0%
ATGGTGATTCGTGTTAGTGT+CGG - chr1.3:8650815-8650834 MS.gene33154:intron 40.0%
ATTAGCAGTGTCCATGTGTA+AGG + chr1.3:8650759-8650778 None:intergenic 40.0%
CAACCTTGAAATGACACATG+AGG + chr1.3:8651539-8651558 None:intergenic 40.0%
CAAGACAAACAAGTAGGTGA+AGG - chr1.3:8651368-8651387 MS.gene33154:intron 40.0%
CAATGTTCATATGCCAGACA+TGG - chr1.3:8649810-8649829 MS.gene33154:CDS 40.0%
CCAGATGGAAAGGATCTAAA+AGG + chr1.3:8651089-8651108 None:intergenic 40.0%
CGATGCTTCGTAACTTCTAA+AGG + chr1.3:8650710-8650729 None:intergenic 40.0%
GAAACCTGTTAGACTGAAAG+AGG - chr1.3:8650043-8650062 MS.gene33154:intron 40.0%
GTTGCTGCGGTTAATCAATT+AGG - chr1.3:8650329-8650348 MS.gene33154:intron 40.0%
TATTGGAATGCGAGTTCTTG+CGG - chr1.3:8649118-8649137 MS.gene33154:CDS 40.0%
TCATTTGGCAGCATGTAATC+AGG - chr1.3:8650072-8650091 MS.gene33154:intron 40.0%
TGAAAATCTGACAAGGGAGA+AGG - chr1.3:8650640-8650659 MS.gene33154:CDS 40.0%
TGCCGATCACATTAGAAGAA+TGG + chr1.3:8651592-8651611 None:intergenic 40.0%
TGCGAATGATGATCCAAAGA+TGG - chr1.3:8649980-8649999 MS.gene33154:intron 40.0%
TGTACCTCTTTCAGTCTAAC+AGG + chr1.3:8650050-8650069 None:intergenic 40.0%
TGTATCTGAACCACTAGCTA+TGG + chr1.3:8650988-8651007 None:intergenic 40.0%
TTACACATGGACACTGCTAA+TGG - chr1.3:8650758-8650777 MS.gene33154:CDS 40.0%
TTTAAGCTCCTTGAGCTTGT+TGG - chr1.3:8649838-8649857 MS.gene33154:CDS 40.0%
TTTAGATCCTTTCCATCTGG+TGG - chr1.3:8651089-8651108 MS.gene33154:intron 40.0%
! ACAGAAGGTGCAATGGTATT+TGG - chr1.3:8651441-8651460 MS.gene33154:intron 40.0%
! CCTTTTAGATCCTTTCCATC+TGG - chr1.3:8651086-8651105 MS.gene33154:intron 40.0%
! CTTGAAAACAGAAGGTGCAA+TGG - chr1.3:8651434-8651453 MS.gene33154:intron 40.0%
! GATCCAAAGATGGTGATGAA+AGG - chr1.3:8649990-8650009 MS.gene33154:intron 40.0%
! GGAAGATAAGAGTTTGTTCC+AGG + chr1.3:8651488-8651507 None:intergenic 40.0%
! GGAGGTTATTGGTCATGTTT+TGG + chr1.3:8651301-8651320 None:intergenic 40.0%
!! ATGAATGGATTGCATTCTGC+CGG - chr1.3:8651152-8651171 MS.gene33154:intron 40.0%
!! ATGCCTCATGTGTCATTTCA+AGG - chr1.3:8651533-8651552 MS.gene33154:intron 40.0%
!! CTCATGTGTCATTTCAAGGT+TGG - chr1.3:8651537-8651556 MS.gene33154:intron 40.0%
!! GTTGATGATGAAGCATACCA+TGG + chr1.3:8649202-8649221 None:intergenic 40.0%
!! TACCATGGTATTGACACCTT+TGG + chr1.3:8649187-8649206 None:intergenic 40.0%
!! TTTCTACAAGTATTGGCTGG+AGG + chr1.3:8649433-8649452 None:intergenic 40.0%
!!! CTAGGGTTTTGGCTAAGTTT+TGG - chr1.3:8649319-8649338 MS.gene33154:CDS 40.0%
AAATCTGACAAGGGAGAAGG+TGG - chr1.3:8650643-8650662 MS.gene33154:CDS 45.0%
ACATGGTGCGTGTGATTATC+TGG - chr1.3:8650019-8650038 MS.gene33154:intron 45.0%
AGAAGAAGCCTCGAACTGTT+TGG - chr1.3:8650282-8650301 MS.gene33154:intron 45.0%
AGCACAATGCAACAACTACC+TGG - chr1.3:8651467-8651486 MS.gene33154:intron 45.0%
ATTTGGATTGCCGCGAGTTA+GGG + chr1.3:8651246-8651265 None:intergenic 45.0%
CACTGCTCAAAACGCGATAA+CGG - chr1.3:8649738-8649757 MS.gene33154:CDS 45.0%
CATAACACCACCAGATGGAA+AGG + chr1.3:8651099-8651118 None:intergenic 45.0%
CTACTCATAACACCACCAGA+TGG + chr1.3:8651104-8651123 None:intergenic 45.0%
GAAATGGGAAACATTCGGCT+GGG - chr1.3:8650147-8650166 MS.gene33154:CDS 45.0%
GCAAGAACTCGCATTCCAAT+AGG + chr1.3:8649119-8649138 None:intergenic 45.0%
GTTCATATGCCAGACATGGA+TGG - chr1.3:8649814-8649833 MS.gene33154:CDS 45.0%
TAACCGCAGCAACAAACTTG+CGG + chr1.3:8650322-8650341 None:intergenic 45.0%
TCTCAAGTCCAACAAGCTCA+AGG + chr1.3:8649849-8649868 None:intergenic 45.0%
TGCTCGACAACATTCATACC+AGG + chr1.3:8651638-8651657 None:intergenic 45.0%
TGTCGAGCAAGAAGGAAACT+TGG - chr1.3:8651649-8651668 MS.gene33154:CDS 45.0%
! TGATGGTGAGGAAGAAAGTG+AGG - chr1.3:8650229-8650248 MS.gene33154:intron 45.0%
!! AAAAAGTACCTGGAGATGGC+TGG + chr1.3:8650669-8650688 None:intergenic 45.0%
!! CTCCAAAGGTGTCAATACCA+TGG - chr1.3:8649182-8649201 MS.gene33154:CDS 45.0%
!! TGAGCTTGTTGGACTTGAGA+TGG - chr1.3:8649849-8649868 MS.gene33154:CDS 45.0%
ACACATGAGGCATAGCAGCA+TGG + chr1.3:8651526-8651545 None:intergenic 50.0%
CATTTGGATTGCCGCGAGTT+AGG + chr1.3:8651247-8651266 None:intergenic 50.0%
CCATGTGTAAGGCTCGTTAC+AGG + chr1.3:8650748-8650767 None:intergenic 50.0%
CCTGTAACGAGCCTTACACA+TGG - chr1.3:8650745-8650764 MS.gene33154:CDS 50.0%
GACGCGCCTAATTCTGATAG+TGG - chr1.3:8650125-8650144 MS.gene33154:CDS 50.0%
GGAAATGGGAAACATTCGGC+TGG - chr1.3:8650146-8650165 MS.gene33154:CDS 50.0%
GGATGCAGAATGCTCAGTGT+CGG - chr1.3:8650836-8650855 MS.gene33154:intron 50.0%
!! ACACCGCAAGTTTGTTGCTG+CGG - chr1.3:8650316-8650335 MS.gene33154:intron 50.0%
!! CGTTATCGCGTTTTGAGCAG+TGG + chr1.3:8649740-8649759 None:intergenic 50.0%
ATGGCAGCAGCCATAGCTAG+TGG - chr1.3:8650975-8650994 MS.gene33154:CDS 55.0%
TCAGCGGACCAAACAGTTCG+AGG + chr1.3:8650293-8650312 None:intergenic 55.0%
TTCCAATCGGCCCTAACTCG+CGG - chr1.3:8651233-8651252 MS.gene33154:intron 55.0%
!! CTGACTCGGCTGAAGAGTTC+CGG + chr1.3:8651174-8651193 None:intergenic 55.0%
GGGAAACATTCGGCTGGGAC+AGG - chr1.3:8650152-8650171 MS.gene33154:CDS 60.0%
GAAGGTGGCCAGCCATCTCC+AGG - chr1.3:8650658-8650677 MS.gene33154:CDS 65.0%
TGCCGCGAGTTAGGGCCGAT+TGG + chr1.3:8651238-8651257 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 8649101 8651700 8649101 ID=MS.gene33154
chr1.3 mRNA 8649101 8651700 8649101 ID=MS.gene33154.t1;Parent=MS.gene33154
chr1.3 exon 8651598 8651700 8651598 ID=MS.gene33154.t1.exon1;Parent=MS.gene33154.t1
chr1.3 CDS 8651598 8651700 8651598 ID=cds.MS.gene33154.t1;Parent=MS.gene33154.t1
chr1.3 exon 8650915 8651067 8650915 ID=MS.gene33154.t1.exon2;Parent=MS.gene33154.t1
chr1.3 CDS 8650915 8651067 8650915 ID=cds.MS.gene33154.t1;Parent=MS.gene33154.t1
chr1.3 exon 8650442 8650828 8650442 ID=MS.gene33154.t1.exon3;Parent=MS.gene33154.t1
chr1.3 CDS 8650442 8650828 8650442 ID=cds.MS.gene33154.t1;Parent=MS.gene33154.t1
chr1.3 exon 8650122 8650198 8650122 ID=MS.gene33154.t1.exon4;Parent=MS.gene33154.t1
chr1.3 CDS 8650122 8650198 8650122 ID=cds.MS.gene33154.t1;Parent=MS.gene33154.t1
chr1.3 exon 8649101 8649880 8649101 ID=MS.gene33154.t1.exon5;Parent=MS.gene33154.t1
chr1.3 CDS 8649101 8649880 8649101 ID=cds.MS.gene33154.t1;Parent=MS.gene33154.t1
Gene Sequence

>MS.gene33154

ATGAATGATCAGTTTCCTATTGGAATGCGAGTTCTTGCGGTTGATGATGATAGAACATGTCTCAAGATTTTGGAGAAACTTCTCCAAAGGTGTCAATACCATGGTATGCTTCATCATCAACATCATATTATTGTTGTTTTTGTTTTATTTTGAAATATTAAAGATGGGTCCTTGATTTTAATCTTGTTTTTTGATGTGACAAATCTAATGATATATAGCTAGGGTTTTGGCTAAGTTTTGGAGATACCTATATTTATGCATATGCTAGATTATTATTTTCAATAAAGTTGGTTCATTTTGAATCAAATATATATTTTTCAATATTATGTCCTCCAGCCAATACTTGTAGAAATCAATTAGATTAGTTTTAAATTTTATTTGATATTGTTTAGGTTCAGACTAAATTATATTTGATCGATCACATTGTTGCTTCTTTCAAAGATTGGACATGATTTCGCTTTTAAAGTTGCAATTTTTTTCAAATATCTTTGTGTGCCTAATCTTTGGTGATCTTATGTTGTTTCTTGGATTTCAATTTTATAGATTTTATATTCTGATTGTATATGCAGTGTATCATTTTTTTCTCTTTCAGAATGATATTTATTTTAGTTATGGTTTGTTAATTTGTTTCAGTAACCACTGCTCAAAACGCGATAACGGCGTTGAACTTGTTGAGAGAAAACAAAAGCAATTTTGACCTAGTAATCAGCAATGTTCATATGCCAGACATGGATGGATTTAAGCTCCTTGAGCTTGTTGGACTTGAGATGGACCTACCTGTCATAAGTAAGTTATGCAATTCCTTCAATCTTATATTATTGATACATGTATAACAAATTATAGTATTTTGATTGCAAAGTGTTTGATTTCAGTGTTTTCTGCGAATGATGATCCAAAGATGGTGATGAAAGGAATTGAACATGGTGCGTGTGATTATCTGGTGAAACCTGTTAGACTGAAAGAGGTACAGATCATTTGGCAGCATGTAATCAGGAAGAAGAAAACTAGCAAAAGAAGCAATCATGACGCGCCTAATTCTGATAGTGGAAATGGGAAACATTCGGCTGGGACAGGAAATTCTGATAAAAATGAAAAGCCATCTAGAAAACGGAAGGATAAGAATGAAGATGATGGTGAGGAAGAAAGTGAGGATGATTATGACAATGATGATCAAACTGCGCAGAAGAAGCCTCGAACTGTTTGGTCCGCTGATTTACACCGCAAGTTTGTTGCTGCGGTTAATCAATTAGGCATTGACAGTACGTTCTTCCCTCTTTCTTCAATGCGCAAAAACTTGTCAGCATAATTTTTATCAATTGCTTATATTCGTCTTTTTCTTTCCTTTTTTCCAGTTTATCAAAATATTTGTGCAGATTATAGCAATCCAATGTATTATTTGAAGACTTCCTCAAATATTCTTTACTAAAAATACTAATGTTTAGCCATGCTACGAATTTTTCCGATTCTGAAGTGTTTTTCTCATGATAGTTGTTATCTTGCAGAGGCTGTACCGAAGAAGATTCTTGAACTGATGAATGTTGAAAATCTGACAAGGGAGAAGGTGGCCAGCCATCTCCAGGTACTTTTTCTTATCTCTTATGCTTTACCTTTAGAAGTTACGAAGCATCGACATAGACATGTAAACCTGTAACGAGCCTTACACATGGACACTGCTAATGGTTTGAATAAATGAAGTAATTGAATTTAACAACATATGGTGATTCGTGTTAGTGTCGGATGCAGAATGCTCAGTGTCGGTGCTACATAAATTATCAGTCTTTTCATTCAAGAAAACTTCCTGTTTTTTAATTTTTCTGCAGAAGTATAGACTCTATCTAAAAAGAATTAGTTGTGCGGAAGACAAACAAGATCATATGGCAGCAGCCATAGCTAGTGGTTCAGATACATCCTATTTAAGAAGTGGAGTTGGAGGACATTTGCATACATTGAATGGCTCTACACAGTTTCATAACCATTATAACAATCCTTTTAGATCCTTTCCATCTGGTGGTGTTATGAGTAGTAGATTCAACACACCTACTAATGTGAATATGAATGGATTGCATTCTGCCGGAACTCTTCAGCCGAGTCAGACACATAATTTAAACAACTTGACAGATGATCAGCTAAAGTTCCAATCGGCCCTAACTCGCGGCAATCCAAATGATGTTCAAAGAGGTATCTCTATTAGTCCTATCCAAAACATGACCAATAACCTCCCAAATTTCAGTTTCACAAACAATCCCTTGATGTTAGAAGGAAATTCTCAAGACAAACAAGTAGGTGAAGGATACAAAAATTTAGCATTGCAGAATTCACAGTTTCTCCCTTTACTTGAAAACAGAAGGTGCAATGGTATTTGGTCAAGCACAATGCAACAACTACCTGGAACAAACTCTTATCTTCCAAGAGAATCTTTTAACCATGCTGCTATGCCTCATGTGTCATTTCAAGGTTGGGATAATAACAATAATCATGATGGAAGTTACCATTCTTCTAATGTGATCGGCAGTTCAATAGGCTCTTCTATGATTCCTGGTATGAATGTTGTCGAGCAAGAAGGAAACTTGGACTACAATTATGGTGATTCGGTACAATGA
Protein sequence

>MS.gene33154.t1

MNDQFPIGMRVLAVDDDRTCLKILEKLLQRCQYHVTTAQNAITALNLLRENKSNFDLVISNVHMPDMDGFKLLELVGLEMDLPVIMFSANDDPKMVMKGIEHGACDYLVKPVRLKEVQIIWQHVIRKKKTSKRSNHDAPNSDSGNGKHSAGTGNSDKNEKPSRKRKDKNEDDGEEESEDDYDNDDQTAQKKPRTVWSADLHRKFVAAVNQLGIDKAVPKKILELMNVENLTREKVASHLQKYRLYLKRISCAEDKQDHMAAAIASGSDTSYLRSGVGGHLHTLNGSTQFHNHYNNPFRSFPSGGVMSSRFNTPTNVNMNGLHSAGTLQPSQTHNLNNLTDDQLKFQSALTRGNPNDVQRGISISPIQNMTNNLPNFSFTNNPLMLEGNSQDKQVGEGYKNLALQNSQFLPLLENRRCNGIWSSTMQQLPGTNSYLPRESFNHAAMPHVSFQGWDNNNNHDGSYHSSNVIGSSIGSSMIPGMNVVEQEGNLDYNYGDSVQ