AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene33155

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene33155.t1 MTR_1g013170 96.525 259 9 0 1 259 1 259 2.06e-170 481
MS.gene33155.t1 MTR_7g117705 82.879 257 42 2 1 256 1 256 6.40e-142 408
MS.gene33155.t1 MTR_1g013180 80.443 271 46 1 1 271 152 415 1.17e-141 415
MS.gene33155.t1 MTR_3g106220 69.708 274 76 3 3 271 6 277 8.32e-124 368
MS.gene33155.t1 MTR_2g450070 59.058 276 101 3 3 269 16 288 2.56e-99 305
MS.gene33155.t1 MTR_2g450070 59.058 276 101 3 3 269 16 288 4.87e-99 305
MS.gene33155.t1 MTR_4g121020 54.613 271 93 3 2 258 28 282 5.14e-94 292
MS.gene33155.t1 MTR_4g121020 54.613 271 93 3 2 258 28 282 5.20e-94 292
MS.gene33155.t1 MTR_2g034960 48.852 305 115 4 1 278 25 315 1.60e-92 286
MS.gene33155.t1 MTR_1g032570 52.809 267 121 2 4 266 10 275 2.96e-91 283
MS.gene33155.t1 MTR_4g131580 56.371 259 106 3 5 263 17 268 7.21e-89 277
MS.gene33155.t1 MTR_8g079940 56.863 255 106 2 3 256 23 274 2.19e-88 273
MS.gene33155.t1 MTR_4g131600 56.589 258 104 4 1 257 16 266 2.62e-88 275
MS.gene33155.t1 MTR_4g131570 54.615 260 112 3 5 263 17 271 4.94e-86 269
MS.gene33155.t1 MTR_1g013160 93.243 148 10 0 67 214 1 148 6.33e-86 254
MS.gene33155.t1 MTR_3g086100 51.254 279 127 3 5 279 13 286 1.77e-84 265
MS.gene33155.t1 MTR_3g102590 51.292 271 75 4 2 269 5 221 6.92e-76 232
MS.gene33155.t1 MTR_3g102590 53.750 240 58 2 2 240 5 192 9.59e-71 217
MS.gene33155.t1 MTR_1g032570 51.691 207 95 2 64 266 1 206 2.36e-64 212
MS.gene33155.t1 MTR_3g102600 46.988 249 70 3 1 249 4 190 8.12e-60 191
MS.gene33155.t1 MTR_3g086100 48.636 220 104 3 64 279 1 215 3.43e-58 194
MS.gene33155.t1 MTR_4g021760 38.043 276 140 5 5 279 11 256 3.22e-56 184
MS.gene33155.t1 MTR_3g102600 46.058 241 68 3 2 242 5 183 1.10e-55 179
MS.gene33155.t1 MTR_4g021790 37.407 270 140 5 2 271 7 247 2.31e-50 167
MS.gene33155.t1 MTR_4g021855 37.652 247 126 3 2 248 7 225 3.75e-50 168
MS.gene33155.t1 MTR_4g021845 38.912 239 118 4 2 240 7 217 4.69e-49 162
MS.gene33155.t1 MTR_4g121020 46.032 189 72 3 84 258 6 178 3.67e-47 166
MS.gene33155.t1 MTR_0450s0040 37.344 241 130 3 9 249 8 227 1.10e-44 155
MS.gene33155.t1 MTR_8g105600 36.000 275 147 7 5 271 9 262 9.42e-40 146
MS.gene33155.t1 MTR_3g102600 41.872 203 56 3 47 249 1 141 1.51e-39 137
MS.gene33155.t1 MTR_6g045327 33.878 245 132 3 4 247 13 228 6.54e-38 138
MS.gene33155.t1 MTR_7g026400 35.841 226 115 3 32 250 14 216 1.98e-37 140
MS.gene33155.t1 MTR_3g102600 40.306 196 55 3 47 242 1 134 1.36e-35 125
MS.gene33155.t1 MTR_5g055260 33.603 247 109 7 2 248 7 198 3.66e-32 120
MS.gene33155.t1 MTR_8g032710 30.040 253 159 5 8 243 20 271 6.80e-29 115
MS.gene33155.t1 MTR_3g092780 34.532 139 86 2 9 143 56 193 3.03e-23 100
MS.gene33155.t1 MTR_3g092780 34.532 139 86 2 9 143 56 193 3.23e-23 100
MS.gene33155.t1 MTR_4g064730 47.788 113 53 2 156 262 94 206 3.32e-23 97.4
MS.gene33155.t1 MTR_6g016850 36.434 129 79 2 1 127 5 132 4.44e-23 93.6
MS.gene33155.t1 MTR_8g024260 34.524 168 96 3 11 164 38 205 9.63e-23 98.6
MS.gene33155.t1 MTR_4g023980 32.530 166 85 3 52 217 36 174 1.13e-22 92.8
MS.gene33155.t1 MTR_3g092780 37.008 127 75 2 9 131 56 181 2.83e-22 97.1
MS.gene33155.t1 MTR_3g092780 37.008 127 75 2 9 131 56 181 3.10e-22 97.1
MS.gene33155.t1 MTR_7g118260 35.484 155 85 4 2 144 5 156 1.37e-21 95.1
MS.gene33155.t1 MTR_7g118260 30.366 191 118 4 2 180 5 192 6.83e-21 92.8
MS.gene33155.t1 MTR_7g089010 53.846 78 36 0 172 249 72 149 2.71e-20 89.0
MS.gene33155.t1 MTR_1g067110 36.885 122 74 2 9 127 85 206 4.76e-20 90.5
MS.gene33155.t1 MTR_1g067110 36.885 122 74 2 9 127 85 206 5.08e-20 90.5
MS.gene33155.t1 MTR_4g061360 36.885 122 74 1 9 127 89 210 1.09e-19 89.7
MS.gene33155.t1 MTR_8g093040 29.098 244 130 4 4 247 13 213 1.18e-19 87.0
MS.gene33155.t1 MTR_6g453220 77.358 53 12 0 207 259 379 431 2.06e-19 88.6
MS.gene33155.t1 MTR_3g037390 33.871 124 79 1 9 129 24 147 8.74e-19 86.7
MS.gene33155.t1 MTR_4g108880 32.258 124 81 1 9 129 33 156 2.80e-17 82.4
MS.gene33155.t1 MTR_5g014040 52.703 74 34 1 184 256 304 377 2.90e-17 82.0
MS.gene33155.t1 MTR_5g014040 35.200 125 75 3 5 127 14 134 1.87e-12 67.8
MS.gene33155.t1 MTR_4g098870 55.072 69 30 1 189 256 308 376 2.54e-16 79.3
MS.gene33155.t1 MTR_4g098870 32.258 124 80 2 5 127 14 134 3.86e-11 63.9
MS.gene33155.t1 MTR_4g098870 55.072 69 30 1 189 256 328 396 2.79e-16 79.3
MS.gene33155.t1 MTR_4g098870 32.258 124 80 2 5 127 34 154 3.82e-11 63.9
MS.gene33155.t1 MTR_4g098870 55.072 69 30 1 189 256 308 376 2.90e-16 79.3
MS.gene33155.t1 MTR_4g098870 32.258 124 80 2 5 127 14 134 5.85e-11 63.2
MS.gene33155.t1 MTR_4g098870 55.072 69 30 1 189 256 347 415 3.03e-16 79.3
MS.gene33155.t1 MTR_4g098870 32.258 124 80 2 5 127 53 173 5.34e-11 63.5
MS.gene33155.t1 MTR_4g098870 55.072 69 30 1 189 256 328 396 3.16e-16 79.3
MS.gene33155.t1 MTR_4g098870 32.258 124 80 2 5 127 34 154 5.63e-11 63.5
MS.gene33155.t1 MTR_5g014040 49.367 79 34 2 184 256 264 342 3.16e-15 76.3
MS.gene33155.t1 MTR_5g014040 49.367 79 34 2 184 256 304 382 3.36e-15 76.3
MS.gene33155.t1 MTR_5g014040 35.200 125 75 3 5 127 14 134 2.06e-12 67.8
MS.gene33155.t1 MTR_4g078810 55.357 56 25 0 188 243 155 210 3.43e-15 75.9
MS.gene33155.t1 MTR_4g086835 41.837 98 44 1 186 270 269 366 4.30e-15 75.5
MS.gene33155.t1 MTR_2g090675 55.357 56 25 0 188 243 158 213 8.41e-15 74.7
MS.gene33155.t1 MTR_1g093080 41.379 87 50 1 177 262 197 283 3.70e-14 72.4
MS.gene33155.t1 MTR_4g113140 52.857 70 31 2 182 250 227 295 6.34e-14 72.0
MS.gene33155.t1 MTR_5g017980 51.613 62 30 0 188 249 189 250 1.02e-13 70.9
MS.gene33155.t1 MTR_5g017980 51.613 62 30 0 188 249 190 251 1.04e-13 70.9
MS.gene33155.t1 MTR_7g115530 64.286 56 19 1 189 243 216 271 1.38e-13 70.1
MS.gene33155.t1 MTR_4g086835 41.414 99 44 2 186 270 269 367 1.49e-13 70.9
MS.gene33155.t1 MTR_7g115530 64.286 56 19 1 189 243 216 271 2.00e-13 70.1
MS.gene33155.t1 MTR_7g115530 54.286 70 31 1 189 257 216 285 2.18e-13 70.1
MS.gene33155.t1 MTR_1g080330 60.345 58 22 1 187 243 171 228 3.02e-13 68.9
MS.gene33155.t1 MTR_7g115530 64.286 56 19 1 189 243 216 271 3.06e-13 70.1
MS.gene33155.t1 MTR_1g080330 60.345 58 22 1 187 243 171 228 4.13e-13 69.3
MS.gene33155.t1 MTR_7g098250 52.941 68 31 1 187 253 258 325 1.00e-12 68.6
MS.gene33155.t1 MTR_2g023580 47.826 92 40 4 190 274 54 144 1.55e-12 66.2
MS.gene33155.t1 MTR_5g054300 43.590 78 43 1 172 248 179 256 1.73e-12 67.4
MS.gene33155.t1 MTR_5g017980 50.794 63 30 1 188 249 190 252 2.13e-12 67.0
MS.gene33155.t1 MTR_1g090670 55.932 59 25 1 187 244 40 98 3.07e-12 66.2
MS.gene33155.t1 MTR_3g093860 33.858 127 69 3 11 122 20 146 4.12e-12 63.5
MS.gene33155.t1 MTR_6g088950 30.645 124 85 1 7 129 5 128 5.10e-12 64.3
MS.gene33155.t1 MTR_7g115530 62.963 54 19 1 189 241 216 269 8.20e-12 64.7
MS.gene33155.t1 MTR_6g032990 54.545 55 24 1 191 244 42 96 8.32e-12 65.5
MS.gene33155.t1 MTR_7g490310 30.216 139 76 3 11 128 11 149 8.91e-12 64.3
MS.gene33155.t1 MTR_2g086450 54.545 55 24 1 191 244 45 99 9.01e-12 65.5
MS.gene33155.t1 MTR_5g027440 56.364 55 23 1 191 244 25 79 1.59e-11 63.9
MS.gene33155.t1 MTR_8g038620 28.986 138 78 3 11 128 11 148 1.60e-11 62.4
MS.gene33155.t1 MTR_5g041350 50.909 55 26 1 191 244 15 69 5.83e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene33155.t1 AT2G25180 63.953 258 87 3 3 259 11 263 3.19e-106 321
MS.gene33155.t1 AT4G31920 58.803 284 95 5 3 285 11 273 3.61e-102 310
MS.gene33155.t1 AT3G16857 49.477 287 127 2 3 271 31 317 1.87e-90 283
MS.gene33155.t1 AT3G16857 49.477 287 127 2 3 271 31 317 4.07e-90 282
MS.gene33155.t1 AT5G58080 55.755 278 97 5 3 271 12 272 1.30e-84 266
MS.gene33155.t1 AT1G67710 54.150 253 109 3 6 254 8 257 5.77e-80 251
MS.gene33155.t1 AT2G01760 46.622 296 148 4 1 286 3 298 9.91e-79 244
MS.gene33155.t1 AT3G16857 48.780 246 108 2 44 271 2 247 4.72e-73 236
MS.gene33155.t1 AT2G01760 45.965 285 119 4 1 250 3 287 9.00e-73 229
MS.gene33155.t1 AT1G67710 52.308 195 86 3 64 254 1 192 1.27e-53 181
MS.gene33155.t1 AT5G49240 38.819 237 142 2 5 240 38 272 9.69e-51 169
MS.gene33155.t1 AT2G01760 43.348 233 122 4 64 286 1 233 5.19e-50 168
MS.gene33155.t1 AT3G62670 35.599 309 156 10 10 292 42 333 3.14e-46 161
MS.gene33155.t1 AT1G67710 46.857 175 86 3 84 254 12 183 9.27e-39 142
MS.gene33155.t1 AT1G49190 31.560 282 174 4 4 274 29 302 2.87e-36 134
MS.gene33155.t1 AT5G07210 31.047 277 149 7 7 250 14 281 3.76e-33 128
MS.gene33155.t1 AT5G07210 30.175 285 149 8 7 250 14 289 1.34e-30 121
MS.gene33155.t1 AT2G27070 29.577 284 145 7 7 249 14 283 2.14e-28 114
MS.gene33155.t1 AT2G46790 39.837 123 69 2 9 127 37 158 5.27e-25 104
MS.gene33155.t1 AT2G46790 39.837 123 69 2 9 127 37 158 5.54e-25 104
MS.gene33155.t1 AT5G59570 52.273 88 42 0 170 257 120 207 1.39e-23 98.2
MS.gene33155.t1 AT5G59570 52.273 88 42 0 170 257 120 207 1.39e-23 98.2
MS.gene33155.t1 AT1G49190 37.600 125 78 0 4 128 29 153 1.74e-23 100
MS.gene33155.t1 AT2G40970 40.678 118 70 0 167 284 81 198 9.00e-23 95.1
MS.gene33155.t1 AT5G24470 36.719 128 76 2 7 130 48 174 2.07e-22 97.4
MS.gene33155.t1 AT3G46640 36.420 162 83 4 104 249 45 202 3.20e-22 95.1
MS.gene33155.t1 AT3G46640 37.107 159 86 4 104 249 45 202 4.02e-22 94.7
MS.gene33155.t1 AT3G46640 37.107 159 86 4 104 249 45 202 4.02e-22 94.7
MS.gene33155.t1 AT5G02810 38.525 122 72 1 9 127 78 199 2.59e-21 94.4
MS.gene33155.t1 AT1G68210 25.632 277 177 3 9 257 13 288 9.25e-21 92.8
MS.gene33155.t1 AT5G05090 53.165 79 37 0 171 249 61 139 1.05e-20 89.7
MS.gene33155.t1 AT5G60100 37.795 127 75 2 5 127 59 185 1.25e-20 91.7
MS.gene33155.t1 AT5G60100 37.795 127 75 2 5 127 59 185 1.25e-20 91.7
MS.gene33155.t1 AT5G60100 37.795 127 75 2 5 127 59 185 1.25e-20 91.7
MS.gene33155.t1 AT5G60100 37.795 127 75 2 5 127 59 185 1.47e-20 92.0
MS.gene33155.t1 AT5G60100 37.795 127 75 2 5 127 59 185 1.47e-20 92.0
MS.gene33155.t1 AT5G60100 37.795 127 75 2 5 127 59 185 1.47e-20 92.0
MS.gene33155.t1 AT5G60100 37.795 127 75 2 5 127 59 185 2.33e-20 91.3
MS.gene33155.t1 AT3G10760 65.000 60 21 0 190 249 104 163 2.52e-20 89.7
MS.gene33155.t1 AT5G44190 40.678 118 64 2 183 296 139 254 5.80e-19 86.7
MS.gene33155.t1 AT5G61380 33.871 124 79 1 9 129 19 142 6.10e-19 87.4
MS.gene33155.t1 AT5G44190 40.678 118 64 2 183 296 139 254 8.19e-19 85.9
MS.gene33155.t1 AT2G20570 44.330 97 43 1 153 249 126 211 1.94e-16 79.3
MS.gene33155.t1 AT2G20570 61.111 54 21 0 196 249 174 227 4.53e-15 75.5
MS.gene33155.t1 AT4G18020 55.932 59 26 0 196 254 301 359 1.29e-14 74.3
MS.gene33155.t1 AT4G18020 55.932 59 26 0 196 254 301 359 1.29e-14 74.3
MS.gene33155.t1 AT4G18020 55.932 59 26 0 196 254 301 359 1.29e-14 74.3
MS.gene33155.t1 AT4G18020 55.932 59 26 0 196 254 301 359 1.29e-14 74.3
MS.gene33155.t1 AT4G18020 55.932 59 26 0 196 254 301 359 1.29e-14 74.3
MS.gene33155.t1 AT4G18020 55.932 59 26 0 196 254 301 359 1.29e-14 74.3
MS.gene33155.t1 AT4G18020 55.932 59 26 0 196 254 301 359 1.50e-14 74.3
MS.gene33155.t1 AT4G18020 55.932 59 26 0 196 254 301 359 1.50e-14 74.3
MS.gene33155.t1 AT4G18020 55.932 59 26 0 196 254 301 359 1.50e-14 74.3
MS.gene33155.t1 AT4G18020 55.932 59 26 0 196 254 301 359 1.50e-14 74.3
MS.gene33155.t1 AT4G18020 55.932 59 26 0 196 254 301 359 1.50e-14 74.3
MS.gene33155.t1 AT2G03500 53.125 64 29 1 188 250 230 293 2.89e-13 70.1
MS.gene33155.t1 AT3G57040 28.395 162 94 2 11 150 11 172 8.49e-13 67.0
MS.gene33155.t1 AT3G57040 28.395 162 94 2 11 150 11 172 8.49e-13 67.0
MS.gene33155.t1 AT5G45580 42.000 100 53 3 181 277 11 108 2.92e-12 66.2
MS.gene33155.t1 AT3G56380 31.298 131 76 2 9 125 20 150 7.29e-12 62.8
MS.gene33155.t1 AT3G04030 54.545 55 24 1 191 244 45 99 7.40e-12 65.9
MS.gene33155.t1 AT3G04030 54.545 55 24 1 191 244 45 99 8.33e-12 65.5
MS.gene33155.t1 AT5G45580 43.182 88 47 2 191 277 7 92 8.35e-12 64.7
MS.gene33155.t1 AT3G04030 51.852 54 26 0 191 244 45 98 1.29e-11 65.1
MS.gene33155.t1 AT1G25550 51.667 60 28 1 190 248 208 267 1.42e-11 64.7
MS.gene33155.t1 AT3G56380 31.538 130 76 2 9 125 20 149 1.46e-11 62.0
MS.gene33155.t1 AT2G40670 32.331 133 74 2 9 125 29 161 2.45e-11 61.6
MS.gene33155.t1 AT1G13300 47.692 65 33 1 185 248 175 239 2.89e-11 63.9
MS.gene33155.t1 AT2G40670 32.576 132 74 2 9 125 29 160 4.79e-11 60.8
MS.gene33155.t1 AT1G49560 45.833 72 38 1 186 256 187 258 5.07e-11 63.2
MS.gene33155.t1 AT4G37180 49.180 61 30 1 189 248 208 268 5.74e-11 62.8
MS.gene33155.t1 AT4G37180 49.180 61 30 1 189 248 215 275 7.01e-11 62.8
MS.gene33155.t1 AT4G13640 38.095 105 55 3 187 281 33 137 8.87e-11 62.0
MS.gene33155.t1 AT4G13640 38.095 105 55 3 187 281 33 137 9.58e-11 62.0

Find 61 sgRNAs with CRISPR-Local

Find 101 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAGAACATGTCTCAAGATTT+TGG 0.179210 1.3:-8657340 MS.gene33155:CDS
ATTCCTTTCATCACCATCTT+TGG 0.256348 1.3:+8656490 None:intergenic
CCATTTCCACTATCAGAATT+AGG 0.318838 1.3:+8656352 None:intergenic
ATATGTGAGACTGAGAAGTA+TGG 0.327860 1.3:+8655478 None:intergenic
ATGAATGATCAGTTTCCTAT+TGG 0.330551 1.3:-8657390 MS.gene33155:CDS
CCTAATTCTGATAGTGGAAA+TGG 0.330629 1.3:-8656352 MS.gene33155:CDS
TTTAAGCTTCTTGAGCTTGT+TGG 0.375182 1.3:-8656645 MS.gene33155:CDS
AAAGGTGGCCAGCCATCTTC+AGG 0.376644 1.3:-8655862 MS.gene33155:intron
ACATGGTGCGTGTGATTATC+TGG 0.387305 1.3:-8656464 MS.gene33155:CDS
TAAAAGAGGTACAGATCATT+TGG 0.388547 1.3:-8656426 MS.gene33155:CDS
TATGAACATTGCTGATTACT+AGG 0.423694 1.3:+8656685 None:intergenic
TACCATGGTATTGACACCTT+TGG 0.431203 1.3:+8657307 None:intergenic
AGAATGTCACTGGGAAAGAT+TGG 0.434197 1.3:+8655512 None:intergenic
TCATTTGGCAGCATGTAATC+AGG 0.452747 1.3:-8656411 MS.gene33155:CDS
GACGCGCCTAATTCTGATAG+TGG 0.455329 1.3:-8656358 MS.gene33155:CDS
AGAAGAAGCCTCGAACTGTT+TGG 0.455763 1.3:-8656201 MS.gene33155:CDS
TGAGCTTGTTGGACTTGAGA+TGG 0.457732 1.3:-8656634 MS.gene33155:CDS
GCAAGAACTCGCATTCCAAT+AGG 0.458411 1.3:+8657375 None:intergenic
AGCTTAAATCCATCCATGTC+TGG 0.471845 1.3:+8656660 None:intergenic
CATATGGAAGCAGCCTTAGC+TGG 0.475370 1.3:-8655565 MS.gene33155:CDS
GTTGTTGCGGTCAATCAATT+AGG 0.476132 1.3:-8656154 MS.gene33155:CDS
GGGAAAGATTCGGCTGGGAC+AGG 0.479650 1.3:-8656331 MS.gene33155:CDS
CTAATTCTGATAGTGGAAAT+GGG 0.482228 1.3:-8656351 MS.gene33155:CDS
CAGCTAAGGCTGCTTCCATA+TGG 0.483151 1.3:+8655566 None:intergenic
TGAAAATCTGACAAGGGAAA+AGG 0.489796 1.3:-8655880 MS.gene33155:CDS
CACTGCTCAAAACGCGATAA+CGG 0.499559 1.3:-8656745 MS.gene33155:CDS
TAGTGGAAATGGGAAAGATT+CGG 0.502507 1.3:-8656341 MS.gene33155:CDS
GTCAATCAATTAGGCATTGA+CGG 0.502566 1.3:-8656145 MS.gene33155:intron
GGGTGGGAAAAGAATGTCAC+TGG 0.506615 1.3:+8655502 None:intergenic
AGCATAAAAGTACCTGAAGA+TGG 0.509866 1.3:+8655850 None:intergenic
ACATCGCAAGTTTGTTGTTG+CGG 0.516543 1.3:-8656167 MS.gene33155:CDS
TATGTGAGACTGAGAAGTAT+GGG 0.518755 1.3:+8655479 None:intergenic
GAAACCTGTTAGACTAAAAG+AGG 0.521944 1.3:-8656440 MS.gene33155:CDS
GGAAATGGGAAAGATTCGGC+TGG 0.530128 1.3:-8656337 MS.gene33155:CDS
TATTGGAATGCGAGTTCTTG+CGG 0.540178 1.3:-8657373 MS.gene33155:CDS
TGCATCTGAACTACCAGCTA+AGG 0.541614 1.3:+8655552 None:intergenic
TTGTATAACTTACTTATGAC+AGG 0.544810 1.3:+8656606 None:intergenic
TAAAAGTACCTGAAGATGGC+TGG 0.546379 1.3:+8655854 None:intergenic
TCAGTGGACCAAACAGTTCG+AGG 0.556377 1.3:+8656193 None:intergenic
CAATGTTCATATGCCAGACA+TGG 0.556391 1.3:-8656673 MS.gene33155:CDS
AGATTGGCTGCTGAGTTGAT+AGG 0.561182 1.3:+8655528 None:intergenic
GGAAGACAGACAAGACCATA+TGG 0.582020 1.3:-8655581 MS.gene33155:CDS
GTTCATATGCCAGACATGGA+TGG 0.584384 1.3:-8656669 MS.gene33155:CDS
ATGTGAGACTGAGAAGTATG+GGG 0.595182 1.3:+8655480 None:intergenic
TGACAGCGAGGAAGAAAATG+AGG 0.600745 1.3:-8656254 MS.gene33155:CDS
TGCGAATGATGATCCAAAGA+TGG 0.615837 1.3:-8656503 MS.gene33155:CDS
GGTCTTGTCTGTCTTCCGCA+CGG 0.622559 1.3:+8655587 None:intergenic
GAAATGGGAAAGATTCGGCT+GGG 0.627002 1.3:-8656336 MS.gene33155:CDS
AAATCTGACAAGGGAAAAGG+TGG 0.629315 1.3:-8655877 MS.gene33155:CDS
GATCCAAAGATGGTGATGAA+AGG 0.637771 1.3:-8656493 MS.gene33155:CDS
ATAACTTACTTATGACAGGT+AGG 0.639228 1.3:+8656610 None:intergenic
TGTGAGACTGAGAAGTATGG+GGG 0.643481 1.3:+8655481 None:intergenic
CTCCAAAGGTGTCAATACCA+TGG 0.650196 1.3:-8657309 MS.gene33155:intron
AAACTTGCGATGTAAATCAG+TGG 0.662856 1.3:+8656177 None:intergenic
TAAGAATGAAGATGACAGCG+AGG 0.681101 1.3:-8656266 MS.gene33155:CDS
GTGAGACTGAGAAGTATGGG+GGG 0.686210 1.3:+8655482 None:intergenic
GAATGTTGAAAATCTGACAA+GGG 0.692411 1.3:-8655886 MS.gene33155:CDS
TGAATGTTGAAAATCTGACA+AGG 0.698854 1.3:-8655887 MS.gene33155:CDS
GGTGGGAAAAGAATGTCACT+GGG 0.701317 1.3:+8655503 None:intergenic
GTGATGAAAGGAATTGAACA+TGG 0.742148 1.3:-8656481 MS.gene33155:CDS
TCTACAAAGAATTAGCCGTG+CGG 0.798557 1.3:-8655602 MS.gene33155:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATGATATTAATTTTAGTTA+TGG - chr1.3:8656062-8656081 MS.gene33155:intron 10.0%
!!! AATTTTATTTGATATTGTTT+AGG - chr1.3:8655835-8655854 MS.gene33155:intron 10.0%
!!! TATTTTGAAATATTAAAGAT+TGG - chr1.3:8655607-8655626 MS.gene33155:CDS 10.0%
!! AAAAAACAAGATTAAAATCA+AGG + chr1.3:8655634-8655653 None:intergenic 15.0%
!!! ATTATTATTTTCAATGAAGT+TGG - chr1.3:8655728-8655747 MS.gene33155:intron 15.0%
!! AAATCTAATGATATATAGCT+AGG - chr1.3:8655662-8655681 MS.gene33155:intron 20.0%
!! AATCTAATGATATATAGCTA+GGG - chr1.3:8655663-8655682 MS.gene33155:intron 20.0%
!! TATCGATAATATAAGATTGA+AGG + chr1.3:8656274-8656293 None:intergenic 20.0%
! ATGAAAAACCATCTAGAAAA+AGG - chr1.3:8656558-8656577 MS.gene33155:intron 25.0%
! CATGATATTAATCTCAACAT+TGG + chr1.3:8656774-8656793 None:intergenic 25.0%
! TTGTATAACTTACTTATGAC+AGG + chr1.3:8656249-8656268 None:intergenic 25.0%
!! GCTAAACATAAGTATTTTTC+GGG + chr1.3:8656852-8656871 None:intergenic 25.0%
!! GGCTAAACATAAGTATTTTT+CGG + chr1.3:8656853-8656872 None:intergenic 25.0%
!! TAATTGATTTCTACAAGCAT+TGG + chr1.3:8655804-8655823 None:intergenic 25.0%
!!! ATCAGTTCAAGAATCTTTTT+TGG + chr1.3:8656946-8656965 None:intergenic 25.0%
AAAACCATCTAGAAAAAGGA+AGG - chr1.3:8656562-8656581 MS.gene33155:intron 30.0%
AATCGGAAAAATTCATAGCA+TGG + chr1.3:8656874-8656893 None:intergenic 30.0%
ATAACTTACTTATGACAGGT+AGG + chr1.3:8656245-8656264 None:intergenic 30.0%
ATGAATGATCAGTTTCCTAT+TGG - chr1.3:8655462-8655481 MS.gene33155:CDS 30.0%
GAATGTTGAAAATCTGACAA+GGG - chr1.3:8656966-8656985 MS.gene33155:intron 30.0%
TGAATGTTGAAAATCTGACA+AGG - chr1.3:8656965-8656984 MS.gene33155:intron 30.0%
TGAGAAAAACACTTCAGAAT+CGG + chr1.3:8656891-8656910 None:intergenic 30.0%
TTCTCGATTCTTTCAAAGAT+TGG - chr1.3:8655888-8655907 MS.gene33155:CDS 30.0%
! ATGATATATAGCTAGGGTTT+TGG - chr1.3:8655669-8655688 MS.gene33155:intron 30.0%
! TAAAAGAGGTACAGATCATT+TGG - chr1.3:8656426-8656445 MS.gene33155:CDS 30.0%
! TAGAACATGTCTCAAGATTT+TGG - chr1.3:8655512-8655531 MS.gene33155:CDS 30.0%
! TATGAACATTGCTGATTACT+AGG + chr1.3:8656170-8656189 None:intergenic 30.0%
! TTATCCTTCCTTTTTCTAGA+TGG + chr1.3:8656569-8656588 None:intergenic 30.0%
!! CTAATTCTGATAGTGGAAAT+GGG - chr1.3:8656501-8656520 MS.gene33155:CDS 30.0%
!!! TTTTTCGGGAAGAATATTTG+AGG + chr1.3:8656838-8656857 None:intergenic 30.0%
AAACTTGCGATGTAAATCAG+TGG + chr1.3:8656678-8656697 None:intergenic 35.0%
AATCTGCACATTGAAGAAAG+AGG + chr1.3:8656743-8656762 None:intergenic 35.0%
ACGTAAGATCACCAAAGATT+AGG + chr1.3:8655964-8655983 None:intergenic 35.0%
AGTTGTTATCTTACTTGCAG+AGG - chr1.3:8656915-8656934 MS.gene33155:intron 35.0%
ATATGTGAGACTGAGAAGTA+TGG + chr1.3:8657377-8657396 None:intergenic 35.0%
ATCTGCACATTGAAGAAAGA+GGG + chr1.3:8656742-8656761 None:intergenic 35.0%
ATTCCTTTCATCACCATCTT+TGG + chr1.3:8656365-8656384 None:intergenic 35.0%
CCATTTCCACTATCAGAATT+AGG + chr1.3:8656503-8656522 None:intergenic 35.0%
GAAACCTGTTAGACTAAAAG+AGG - chr1.3:8656412-8656431 MS.gene33155:CDS 35.0%
GTGATGAAAGGAATTGAACA+TGG - chr1.3:8656371-8656390 MS.gene33155:CDS 35.0%
TAGTGGAAATGGGAAAGATT+CGG - chr1.3:8656511-8656530 MS.gene33155:CDS 35.0%
TGAAAATCTGACAAGGGAAA+AGG - chr1.3:8656972-8656991 MS.gene33155:intron 35.0%
TTTAAGCTTCTTGAGCTTGT+TGG - chr1.3:8656207-8656226 MS.gene33155:CDS 35.0%
! TATGTGAGACTGAGAAGTAT+GGG + chr1.3:8657376-8657395 None:intergenic 35.0%
! TCTTTTGTGTGCCTAATCTT+TGG - chr1.3:8655950-8655969 MS.gene33155:intron 35.0%
! TGTACCTCTTTTAGTCTAAC+AGG + chr1.3:8656419-8656438 None:intergenic 35.0%
!! AGCATAAAAGTACCTGAAGA+TGG + chr1.3:8657005-8657024 None:intergenic 35.0%
!! CCTAATTCTGATAGTGGAAA+TGG - chr1.3:8656500-8656519 MS.gene33155:CDS 35.0%
!! GTCAATCAATTAGGCATTGA+CGG - chr1.3:8656707-8656726 MS.gene33155:CDS 35.0%
!! TTTTGGAGAAACTTCTCCAA+AGG - chr1.3:8655529-8655548 MS.gene33155:CDS 35.0%
AAATCTGACAAGGGAAAAGG+TGG - chr1.3:8656975-8656994 MS.gene33155:intron 40.0%
AGAATGTCACTGGGAAAGAT+TGG + chr1.3:8657343-8657362 None:intergenic 40.0%
AGCTTAAATCCATCCATGTC+TGG + chr1.3:8656195-8656214 None:intergenic 40.0%
ATTAACAGTGTCCTTGTGTC+AGG + chr1.3:8657075-8657094 None:intergenic 40.0%
CAATGTTCATATGCCAGACA+TGG - chr1.3:8656179-8656198 MS.gene33155:CDS 40.0%
GTTGTTGCGGTCAATCAATT+AGG - chr1.3:8656698-8656717 MS.gene33155:CDS 40.0%
TAAGAATGAAGATGACAGCG+AGG - chr1.3:8656586-8656605 MS.gene33155:intron 40.0%
TATTGGAATGCGAGTTCTTG+CGG - chr1.3:8655479-8655498 MS.gene33155:CDS 40.0%
TCATTTGGCAGCATGTAATC+AGG - chr1.3:8656441-8656460 MS.gene33155:CDS 40.0%
TCTACAAAGAATTAGCCGTG+CGG - chr1.3:8657250-8657269 MS.gene33155:intron 40.0%
TGACACAAGGACACTGTTAA+TGG - chr1.3:8657074-8657093 MS.gene33155:intron 40.0%
TGCGAATGATGATCCAAAGA+TGG - chr1.3:8656349-8656368 MS.gene33155:CDS 40.0%
! ATGTGAGACTGAGAAGTATG+GGG + chr1.3:8657375-8657394 None:intergenic 40.0%
! GATCCAAAGATGGTGATGAA+AGG - chr1.3:8656359-8656378 MS.gene33155:CDS 40.0%
!! ACATCGCAAGTTTGTTGTTG+CGG - chr1.3:8656685-8656704 MS.gene33155:CDS 40.0%
!! GGTGATCTTACGTTGTTTCT+TGG - chr1.3:8655971-8655990 MS.gene33155:intron 40.0%
!! GTTGATGATGAAGCATACCA+TGG + chr1.3:8655563-8655582 None:intergenic 40.0%
!! TAAAAGTACCTGAAGATGGC+TGG + chr1.3:8657001-8657020 None:intergenic 40.0%
!! TACCATGGTATTGACACCTT+TGG + chr1.3:8655548-8655567 None:intergenic 40.0%
!!! CTAGGGTTTTGGCTAAGTTT+TGG - chr1.3:8655680-8655699 MS.gene33155:intron 40.0%
ACATGGTGCGTGTGATTATC+TGG - chr1.3:8656388-8656407 MS.gene33155:CDS 45.0%
AGAAGAAGCCTCGAACTGTT+TGG - chr1.3:8656651-8656670 MS.gene33155:CDS 45.0%
CACTGCTCAAAACGCGATAA+CGG - chr1.3:8656107-8656126 MS.gene33155:intron 45.0%
CCTATAATGAGCCTGACACA+AGG - chr1.3:8657061-8657080 MS.gene33155:intron 45.0%
CCTTGTGTCAGGCTCATTAT+AGG + chr1.3:8657064-8657083 None:intergenic 45.0%
CGTTGATGTCGTGTTAGTGT+CGG - chr1.3:8657130-8657149 MS.gene33155:intron 45.0%
GAAATGGGAAAGATTCGGCT+GGG - chr1.3:8656516-8656535 MS.gene33155:intron 45.0%
GCAAGAACTCGCATTCCAAT+AGG + chr1.3:8655480-8655499 None:intergenic 45.0%
GGAAGACAGACAAGACCATA+TGG - chr1.3:8657271-8657290 MS.gene33155:intron 45.0%
GGTGGGAAAAGAATGTCACT+GGG + chr1.3:8657352-8657371 None:intergenic 45.0%
GTTCATATGCCAGACATGGA+TGG - chr1.3:8656183-8656202 MS.gene33155:CDS 45.0%
TGACAGCGAGGAAGAAAATG+AGG - chr1.3:8656598-8656617 MS.gene33155:intron 45.0%
TGCATCTGAACTACCAGCTA+AGG + chr1.3:8657303-8657322 None:intergenic 45.0%
! TGTGAGACTGAGAAGTATGG+GGG + chr1.3:8657374-8657393 None:intergenic 45.0%
!! AGATTGGCTGCTGAGTTGAT+AGG + chr1.3:8657327-8657346 None:intergenic 45.0%
!! CTCCAAAGGTGTCAATACCA+TGG - chr1.3:8655543-8655562 MS.gene33155:CDS 45.0%
!! TGAGCTTGTTGGACTTGAGA+TGG - chr1.3:8656218-8656237 MS.gene33155:CDS 45.0%
CAGCTAAGGCTGCTTCCATA+TGG + chr1.3:8657289-8657308 None:intergenic 50.0%
CATATGGAAGCAGCCTTAGC+TGG - chr1.3:8657287-8657306 MS.gene33155:intron 50.0%
GACGCGCCTAATTCTGATAG+TGG - chr1.3:8656494-8656513 MS.gene33155:CDS 50.0%
GGAAATGGGAAAGATTCGGC+TGG - chr1.3:8656515-8656534 MS.gene33155:intron 50.0%
GGGTGGGAAAAGAATGTCAC+TGG + chr1.3:8657353-8657372 None:intergenic 50.0%
TCAGTGGACCAAACAGTTCG+AGG + chr1.3:8656662-8656681 None:intergenic 50.0%
! GTGAGACTGAGAAGTATGGG+GGG + chr1.3:8657373-8657392 None:intergenic 50.0%
!! CGTTATCGCGTTTTGAGCAG+TGG + chr1.3:8656109-8656128 None:intergenic 50.0%
AAAGGTGGCCAGCCATCTTC+AGG - chr1.3:8656990-8657009 MS.gene33155:intron 55.0%
! AGACTGAGAAGTATGGGGGG+TGG + chr1.3:8657370-8657389 None:intergenic 55.0%
! GACTGAGAAGTATGGGGGGT+GGG + chr1.3:8657369-8657388 None:intergenic 55.0%
! GGTCTTGTCTGTCTTCCGCA+CGG + chr1.3:8657268-8657287 None:intergenic 55.0%
GGACGCAGAATGCACAGCGT+CGG - chr1.3:8657151-8657170 MS.gene33155:intron 60.0%
GGGAAAGATTCGGCTGGGAC+AGG - chr1.3:8656521-8656540 MS.gene33155:intron 60.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 8655462 8657412 8655462 ID=MS.gene33155
chr1.3 mRNA 8655462 8657412 8655462 ID=MS.gene33155.t1;Parent=MS.gene33155
chr1.3 exon 8657310 8657412 8657310 ID=MS.gene33155.t1.exon1;Parent=MS.gene33155.t1
chr1.3 CDS 8657310 8657412 8657310 ID=cds.MS.gene33155.t1;Parent=MS.gene33155.t1
chr1.3 exon 8656619 8656771 8656619 ID=MS.gene33155.t1.exon2;Parent=MS.gene33155.t1
chr1.3 CDS 8656619 8656771 8656619 ID=cds.MS.gene33155.t1;Parent=MS.gene33155.t1
chr1.3 exon 8656146 8656532 8656146 ID=MS.gene33155.t1.exon3;Parent=MS.gene33155.t1
chr1.3 CDS 8656146 8656532 8656146 ID=cds.MS.gene33155.t1;Parent=MS.gene33155.t1
chr1.3 exon 8655863 8655939 8655863 ID=MS.gene33155.t1.exon4;Parent=MS.gene33155.t1
chr1.3 CDS 8655863 8655939 8655863 ID=cds.MS.gene33155.t1;Parent=MS.gene33155.t1
chr1.3 exon 8655462 8655638 8655462 ID=MS.gene33155.t1.exon5;Parent=MS.gene33155.t1
chr1.3 CDS 8655462 8655638 8655462 ID=cds.MS.gene33155.t1;Parent=MS.gene33155.t1
Gene Sequence

>MS.gene33155

ATGAATGATCAGTTTCCTATTGGAATGCGAGTTCTTGCGGTTGATGATGATAGAACATGTCTCAAGATTTTGGAGAAACTTCTCCAAAGGTGTCAATACCATGGTATGCTTCATCATCAACATCATATTATTGTTGTTTTTGTTTTATTTTGAAATATTAAAGATTGGTCCTTGATTTTAATCTTGTTTTTTGATGTGACAAATCTAATGATATATAGCTAGGGTTTTGGCTAAGTTTTGGATACATATATTTATGCATATGTTAGATTATTATTTTCAATGAAGTTGGTTCATTTTGAATCAAATATCTATTTTTCAATATTATGTTCAACACTACATCCAATGCTTGTAGAAATCAATTAGATTAATTTTAAATTTTATTTGATATTGTTTAGGTTTAGACTAAATTATATTTGATCGATCACATTCTCGATTCTTTCAAAGATTGGACATGATTTTGCTTTTAAAGTTGCAATTTTTTTTAAATATCTTTTGTGTGCCTAATCTTTGGTGATCTTACGTTGTTTCTTGGATTTCAAAACATTTCGTAGATTTTATGTTCTGATTGTATATGCAGCGTATAATTTTTTTCTCTTTGAGAATGATATTAATTTTAGTTATGGTTTGTTAATTTGTTTCAGTAACCACTGCTCAAAACGCGATAACGGCGTTGAACTTGTTGAGAGAAAACAAAAGCAATTTTGACCTAGTAATCAGCAATGTTCATATGCCAGACATGGATGGATTTAAGCTTCTTGAGCTTGTTGGACTTGAGATGGACCTACCTGTCATAAGTAAGTTATACAATTCCTTCAATCTTATATTATCGATACATGTATAACAAATTATAGTATTTTGATTACGAAGTGTTTGATTTCAGTGTTTTCTGCGAATGATGATCCAAAGATGGTGATGAAAGGAATTGAACATGGTGCGTGTGATTATCTGGTGAAACCTGTTAGACTAAAAGAGGTACAGATCATTTGGCAGCATGTAATCAGGAAGAAGAAAACTAGCAAACGAAGCAATCATGACGCGCCTAATTCTGATAGTGGAAATGGGAAAGATTCGGCTGGGACAGGAAATTCTGATAAGAATGAAAAACCATCTAGAAAAAGGAAGGATAAGAATGAAGATGACAGCGAGGAAGAAAATGAGGATGATTATGACAATGATGATCAAACTGCGCAGAAGAAGCCTCGAACTGTTTGGTCCACTGATTTACATCGCAAGTTTGTTGTTGCGGTCAATCAATTAGGCATTGACGGTACGTTCTTCCCTCTTTCTTCAATGTGCAGATTATAACGATCCAATGTTGAGATTAATATCATGATATAAAAATAAAAATTCTACTTTGTATTATTTGAAGACTTCCTCAAATATTCTTCCCGAAAAATACTTATGTTTAGCCATGCTATGAATTTTTCCGATTCTGAAGTGTTTTTCTCATGATAGTTGTTATCTTACTTGCAGAGGCTGTACCAAAAAAGATTCTTGAACTGATGAATGTTGAAAATCTGACAAGGGAAAAGGTGGCCAGCCATCTTCAGGTACTTTTATGCTTTAGCGTTATAAGTTACGAAGATATAGACACGTAAACCTATAATGAGCCTGACACAAGGACACTGTTAATGGTTTGATTGAATGAAGTAATTGAATTTAATCACACGTTGATGTCGTGTTAGTGTCGGACGCAGAATGCACAGCGTCGGTGCTACACAAATTATCTGTCTTTTCATTCAAGAAAACTTACTGTTTTTTAATTTTTCTGCAGAAGTATAGACTCTATCTACAAAGAATTAGCCGTGCGGAAGACAGACAAGACCATATGGAAGCAGCCTTAGCTGGTAGTTCAGATGCATCCTATCAACTCAGCAGCCAATCTTTCCCAGTGACATTCTTTTCCCACCCCCCATACTTCTCAGTCTCACATATCTTTCATGATTTGTAA
Protein sequence

>MS.gene33155.t1

MNDQFPIGMRVLAVDDDRTCLKILEKLLQRCQYHVTTAQNAITALNLLRENKSNFDLVISNVHMPDMDGFKLLELVGLEMDLPVIMFSANDDPKMVMKGIEHGACDYLVKPVRLKEVQIIWQHVIRKKKTSKRSNHDAPNSDSGNGKDSAGTGNSDKNEKPSRKRKDKNEDDSEEENEDDYDNDDQTAQKKPRTVWSTDLHRKFVVAVNQLGIDEAVPKKILELMNVENLTREKVASHLQKYRLYLQRISRAEDRQDHMEAALAGSSDASYQLSSQSFPVTFFSHPPYFSVSHIFHDL