AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene33156

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene33156.t1 MTR_1g013170 96.310 271 10 0 1 271 1 271 1.27e-170 482
MS.gene33156.t1 MTR_7g117705 80.524 267 50 2 1 266 1 266 3.67e-142 409
MS.gene33156.t1 MTR_1g013180 80.443 271 46 1 1 271 152 415 7.39e-142 415
MS.gene33156.t1 MTR_3g106220 69.091 275 78 3 2 271 5 277 3.43e-123 366
MS.gene33156.t1 MTR_2g450070 58.696 276 102 3 3 269 16 288 1.52e-98 303
MS.gene33156.t1 MTR_2g450070 58.696 276 102 3 3 269 16 288 3.06e-98 303
MS.gene33156.t1 MTR_4g121020 53.169 284 100 4 2 271 28 292 1.97e-94 293
MS.gene33156.t1 MTR_4g121020 53.169 284 100 4 2 271 28 292 2.26e-94 293
MS.gene33156.t1 MTR_2g034960 49.825 285 119 2 1 275 25 295 8.70e-93 287
MS.gene33156.t1 MTR_1g032570 53.846 260 115 2 4 259 10 268 3.95e-91 283
MS.gene33156.t1 MTR_4g131580 56.371 259 106 3 5 263 17 268 6.41e-89 277
MS.gene33156.t1 MTR_4g131600 56.589 258 104 4 1 257 16 266 2.14e-88 275
MS.gene33156.t1 MTR_8g079940 56.863 255 106 2 3 256 23 274 2.17e-88 273
MS.gene33156.t1 MTR_4g131570 52.985 268 124 2 5 271 17 283 3.33e-86 270
MS.gene33156.t1 MTR_1g013160 93.243 148 10 0 67 214 1 148 3.36e-86 255
MS.gene33156.t1 MTR_3g086100 51.254 279 127 3 5 279 13 286 1.12e-84 265
MS.gene33156.t1 MTR_3g102590 51.292 271 75 4 2 269 5 221 6.41e-76 232
MS.gene33156.t1 MTR_3g102590 53.750 240 58 2 2 240 5 192 8.06e-71 218
MS.gene33156.t1 MTR_1g032570 53.000 200 89 2 64 259 1 199 4.18e-64 211
MS.gene33156.t1 MTR_3g102600 46.988 249 70 3 1 249 4 190 7.61e-60 191
MS.gene33156.t1 MTR_3g086100 48.636 220 104 3 64 279 1 215 1.92e-58 195
MS.gene33156.t1 MTR_4g021760 38.406 276 139 5 5 279 11 256 8.40e-57 186
MS.gene33156.t1 MTR_3g102600 46.058 241 68 3 2 242 5 183 9.88e-56 179
MS.gene33156.t1 MTR_4g021790 37.407 270 140 5 2 271 7 247 2.28e-50 167
MS.gene33156.t1 MTR_4g021855 37.652 247 126 3 2 248 7 225 3.40e-50 168
MS.gene33156.t1 MTR_4g021845 38.912 239 118 4 2 240 7 217 4.85e-49 162
MS.gene33156.t1 MTR_4g121020 44.554 202 79 4 84 271 6 188 1.85e-47 167
MS.gene33156.t1 MTR_0450s0040 37.344 241 130 3 9 249 8 227 8.07e-45 155
MS.gene33156.t1 MTR_8g105600 36.000 275 147 7 5 271 9 262 6.69e-40 147
MS.gene33156.t1 MTR_3g102600 41.872 203 56 3 47 249 1 141 1.61e-39 137
MS.gene33156.t1 MTR_6g045327 33.878 245 132 3 4 247 13 228 5.84e-38 138
MS.gene33156.t1 MTR_7g026400 34.855 241 127 3 32 265 14 231 1.67e-37 140
MS.gene33156.t1 MTR_3g102600 40.306 196 55 3 47 242 1 134 1.34e-35 125
MS.gene33156.t1 MTR_5g055260 33.603 247 109 7 2 248 7 198 2.94e-32 120
MS.gene33156.t1 MTR_8g032710 30.040 253 159 5 8 243 20 271 7.01e-29 115
MS.gene33156.t1 MTR_4g064730 47.788 113 53 2 156 262 94 206 2.69e-23 97.8
MS.gene33156.t1 MTR_3g092780 34.532 139 86 2 9 143 56 193 2.70e-23 100
MS.gene33156.t1 MTR_3g092780 34.532 139 86 2 9 143 56 193 3.26e-23 100
MS.gene33156.t1 MTR_6g016850 36.434 129 79 2 1 127 5 132 4.31e-23 93.6
MS.gene33156.t1 MTR_8g024260 34.524 168 96 3 11 164 38 205 1.05e-22 98.6
MS.gene33156.t1 MTR_4g023980 32.530 166 85 3 52 217 36 174 1.12e-22 92.8
MS.gene33156.t1 MTR_3g092780 37.008 127 75 2 9 131 56 181 2.75e-22 97.1
MS.gene33156.t1 MTR_3g092780 37.008 127 75 2 9 131 56 181 3.07e-22 97.1
MS.gene33156.t1 MTR_7g118260 35.484 155 85 4 2 144 5 156 1.49e-21 95.1
MS.gene33156.t1 MTR_7g118260 30.366 191 118 4 2 180 5 192 6.96e-21 92.8
MS.gene33156.t1 MTR_7g089010 53.846 78 36 0 172 249 72 149 2.45e-20 89.4
MS.gene33156.t1 MTR_1g067110 36.885 122 74 2 9 127 85 206 4.90e-20 90.5
MS.gene33156.t1 MTR_1g067110 36.885 122 74 2 9 127 85 206 5.18e-20 90.5
MS.gene33156.t1 MTR_4g061360 36.885 122 74 1 9 127 89 210 1.05e-19 89.7
MS.gene33156.t1 MTR_8g093040 29.098 244 130 4 4 247 13 213 1.22e-19 87.0
MS.gene33156.t1 MTR_6g453220 77.358 53 12 0 207 259 379 431 2.02e-19 88.6
MS.gene33156.t1 MTR_3g037390 33.871 124 79 1 9 129 24 147 9.42e-19 86.7
MS.gene33156.t1 MTR_5g014040 52.703 74 34 1 184 256 304 377 2.80e-17 82.4
MS.gene33156.t1 MTR_5g014040 35.200 125 75 3 5 127 14 134 2.02e-12 67.8
MS.gene33156.t1 MTR_4g108880 32.258 124 81 1 9 129 33 156 3.13e-17 82.0
MS.gene33156.t1 MTR_4g098870 55.072 69 30 1 189 256 308 376 2.42e-16 79.3
MS.gene33156.t1 MTR_4g098870 32.258 124 80 2 5 127 14 134 4.04e-11 63.9
MS.gene33156.t1 MTR_4g098870 55.072 69 30 1 189 256 308 376 2.67e-16 79.3
MS.gene33156.t1 MTR_4g098870 32.258 124 80 2 5 127 14 134 5.64e-11 63.5
MS.gene33156.t1 MTR_4g098870 55.072 69 30 1 189 256 328 396 2.69e-16 79.3
MS.gene33156.t1 MTR_4g098870 32.258 124 80 2 5 127 34 154 4.26e-11 63.9
MS.gene33156.t1 MTR_4g098870 55.072 69 30 1 189 256 347 415 2.97e-16 79.3
MS.gene33156.t1 MTR_4g098870 32.258 124 80 2 5 127 53 173 5.59e-11 63.5
MS.gene33156.t1 MTR_4g098870 55.072 69 30 1 189 256 328 396 3.08e-16 79.3
MS.gene33156.t1 MTR_4g098870 32.258 124 80 2 5 127 34 154 5.74e-11 63.5
MS.gene33156.t1 MTR_5g014040 49.367 79 34 2 184 256 264 342 2.91e-15 76.3
MS.gene33156.t1 MTR_4g078810 55.357 56 25 0 188 243 155 210 3.21e-15 75.9
MS.gene33156.t1 MTR_5g014040 49.367 79 34 2 184 256 304 382 3.32e-15 76.3
MS.gene33156.t1 MTR_5g014040 35.200 125 75 3 5 127 14 134 2.22e-12 67.8
MS.gene33156.t1 MTR_4g086835 50.746 67 33 0 186 252 269 335 7.83e-15 74.7
MS.gene33156.t1 MTR_2g090675 55.357 56 25 0 188 243 158 213 7.88e-15 74.7
MS.gene33156.t1 MTR_1g093080 41.379 87 50 1 177 262 197 283 3.60e-14 72.4
MS.gene33156.t1 MTR_4g113140 52.857 70 31 2 182 250 227 295 6.06e-14 72.0
MS.gene33156.t1 MTR_5g017980 51.613 62 30 0 188 249 190 251 9.83e-14 70.9
MS.gene33156.t1 MTR_5g017980 51.613 62 30 0 188 249 189 250 9.85e-14 70.9
MS.gene33156.t1 MTR_7g115530 64.286 56 19 1 189 243 216 271 1.26e-13 70.5
MS.gene33156.t1 MTR_7g115530 64.286 56 19 1 189 243 216 271 1.79e-13 70.1
MS.gene33156.t1 MTR_7g115530 54.286 70 31 1 189 257 216 285 1.84e-13 70.1
MS.gene33156.t1 MTR_7g115530 64.286 56 19 1 189 243 216 271 2.76e-13 70.1
MS.gene33156.t1 MTR_1g080330 60.345 58 22 1 187 243 171 228 2.85e-13 68.9
MS.gene33156.t1 MTR_4g086835 50.000 68 33 1 186 252 269 336 2.94e-13 70.1
MS.gene33156.t1 MTR_1g080330 60.345 58 22 1 187 243 171 228 3.73e-13 69.7
MS.gene33156.t1 MTR_2g023580 47.826 92 40 4 190 274 54 144 8.06e-13 67.0
MS.gene33156.t1 MTR_7g098250 52.941 68 31 1 187 253 258 325 8.98e-13 68.6
MS.gene33156.t1 MTR_5g054300 43.590 78 43 1 172 248 179 256 1.55e-12 67.4
MS.gene33156.t1 MTR_5g017980 50.794 63 30 1 188 249 190 252 2.11e-12 67.0
MS.gene33156.t1 MTR_1g090670 55.932 59 25 1 187 244 40 98 2.96e-12 66.2
MS.gene33156.t1 MTR_3g093860 35.385 130 63 5 11 122 20 146 4.12e-12 63.5
MS.gene33156.t1 MTR_6g088950 30.645 124 85 1 7 129 5 128 5.41e-12 64.3
MS.gene33156.t1 MTR_7g115530 62.963 54 19 1 189 241 216 269 7.45e-12 64.7
MS.gene33156.t1 MTR_2g086450 54.545 55 24 1 191 244 45 99 8.07e-12 65.5
MS.gene33156.t1 MTR_6g032990 54.545 55 24 1 191 244 42 96 8.10e-12 65.5
MS.gene33156.t1 MTR_7g490310 30.216 139 76 3 11 128 11 149 8.83e-12 64.3
MS.gene33156.t1 MTR_5g027440 56.364 55 23 1 191 244 25 79 1.49e-11 63.9
MS.gene33156.t1 MTR_8g038620 28.986 138 78 3 11 128 11 148 1.51e-11 62.4
MS.gene33156.t1 MTR_5g041350 50.909 55 26 1 191 244 15 69 5.42e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene33156.t1 AT2G25180 63.953 258 87 3 3 259 11 263 2.60e-106 322
MS.gene33156.t1 AT4G31920 58.803 284 95 5 3 285 11 273 1.85e-102 310
MS.gene33156.t1 AT3G16857 49.477 287 127 2 3 271 31 317 5.05e-91 284
MS.gene33156.t1 AT3G16857 49.477 287 127 2 3 271 31 317 1.02e-90 284
MS.gene33156.t1 AT5G58080 55.396 278 98 5 3 271 12 272 7.64e-84 265
MS.gene33156.t1 AT1G67710 54.150 253 109 3 6 254 8 257 4.56e-80 252
MS.gene33156.t1 AT2G01760 47.138 297 138 5 1 282 3 295 4.60e-79 245
MS.gene33156.t1 AT3G16857 48.780 246 108 2 44 271 2 247 8.88e-74 238
MS.gene33156.t1 AT2G01760 43.077 325 138 6 1 282 3 323 4.67e-73 230
MS.gene33156.t1 AT1G67710 52.308 195 86 3 64 254 1 192 1.01e-53 181
MS.gene33156.t1 AT5G49240 38.819 237 142 2 5 240 38 272 1.01e-50 169
MS.gene33156.t1 AT2G01760 44.017 234 112 5 64 282 1 230 3.01e-50 169
MS.gene33156.t1 AT3G62670 34.628 309 159 9 10 292 42 333 1.34e-46 162
MS.gene33156.t1 AT1G67710 46.857 175 86 3 84 254 12 183 7.54e-39 142
MS.gene33156.t1 AT1G49190 31.206 282 175 4 4 274 29 302 2.46e-35 132
MS.gene33156.t1 AT5G07210 31.047 277 149 7 7 250 14 281 3.51e-33 128
MS.gene33156.t1 AT5G07210 30.175 285 149 8 7 250 14 289 1.43e-30 121
MS.gene33156.t1 AT2G27070 29.577 284 145 7 7 249 14 283 2.42e-28 114
MS.gene33156.t1 AT2G46790 39.837 123 69 2 9 127 37 158 5.22e-25 104
MS.gene33156.t1 AT2G46790 39.837 123 69 2 9 127 37 158 5.22e-25 104
MS.gene33156.t1 AT5G59570 48.214 112 50 2 170 278 120 226 9.33e-24 98.6
MS.gene33156.t1 AT5G59570 48.214 112 50 2 170 278 120 226 9.33e-24 98.6
MS.gene33156.t1 AT2G40970 41.525 118 69 0 167 284 81 198 1.69e-23 97.1
MS.gene33156.t1 AT1G49190 37.600 125 78 0 4 128 29 153 1.72e-23 100
MS.gene33156.t1 AT5G24470 36.719 128 76 2 7 130 48 174 2.11e-22 97.4
MS.gene33156.t1 AT3G46640 37.037 162 82 5 104 249 45 202 3.78e-22 94.7
MS.gene33156.t1 AT3G46640 37.037 162 82 5 104 249 45 202 3.78e-22 94.7
MS.gene33156.t1 AT3G46640 36.420 162 83 4 104 249 45 202 3.80e-22 94.7
MS.gene33156.t1 AT5G02810 38.525 122 72 1 9 127 78 199 2.59e-21 94.4
MS.gene33156.t1 AT5G05090 53.165 79 37 0 171 249 61 139 8.93e-21 89.7
MS.gene33156.t1 AT1G68210 25.632 277 177 3 9 257 13 288 9.61e-21 92.8
MS.gene33156.t1 AT5G60100 37.795 127 75 2 5 127 59 185 1.43e-20 91.3
MS.gene33156.t1 AT5G60100 37.795 127 75 2 5 127 59 185 1.43e-20 91.3
MS.gene33156.t1 AT5G60100 37.795 127 75 2 5 127 59 185 1.43e-20 91.3
MS.gene33156.t1 AT5G60100 37.795 127 75 2 5 127 59 185 1.54e-20 91.7
MS.gene33156.t1 AT5G60100 37.795 127 75 2 5 127 59 185 1.54e-20 91.7
MS.gene33156.t1 AT5G60100 37.795 127 75 2 5 127 59 185 1.54e-20 91.7
MS.gene33156.t1 AT3G10760 65.000 60 21 0 190 249 104 163 2.38e-20 89.7
MS.gene33156.t1 AT5G60100 37.795 127 75 2 5 127 59 185 2.54e-20 91.3
MS.gene33156.t1 AT5G44190 40.678 118 64 2 183 296 139 254 3.75e-19 87.0
MS.gene33156.t1 AT5G44190 40.678 118 64 2 183 296 139 254 5.76e-19 86.3
MS.gene33156.t1 AT5G61380 33.871 124 79 1 9 129 19 142 6.64e-19 87.4
MS.gene33156.t1 AT2G20570 44.330 97 43 1 153 249 126 211 1.96e-16 79.3
MS.gene33156.t1 AT2G20570 61.111 54 21 0 196 249 174 227 4.57e-15 75.5
MS.gene33156.t1 AT4G18020 55.932 59 26 0 196 254 301 359 1.27e-14 74.3
MS.gene33156.t1 AT4G18020 55.932 59 26 0 196 254 301 359 1.27e-14 74.3
MS.gene33156.t1 AT4G18020 55.932 59 26 0 196 254 301 359 1.27e-14 74.3
MS.gene33156.t1 AT4G18020 55.932 59 26 0 196 254 301 359 1.27e-14 74.3
MS.gene33156.t1 AT4G18020 55.932 59 26 0 196 254 301 359 1.27e-14 74.3
MS.gene33156.t1 AT4G18020 55.932 59 26 0 196 254 301 359 1.27e-14 74.3
MS.gene33156.t1 AT4G18020 55.932 59 26 0 196 254 301 359 1.47e-14 74.3
MS.gene33156.t1 AT4G18020 55.932 59 26 0 196 254 301 359 1.47e-14 74.3
MS.gene33156.t1 AT4G18020 55.932 59 26 0 196 254 301 359 1.47e-14 74.3
MS.gene33156.t1 AT4G18020 55.932 59 26 0 196 254 301 359 1.47e-14 74.3
MS.gene33156.t1 AT4G18020 55.932 59 26 0 196 254 301 359 1.47e-14 74.3
MS.gene33156.t1 AT2G03500 53.125 64 29 1 188 250 230 293 2.73e-13 70.1
MS.gene33156.t1 AT3G57040 28.395 162 94 2 11 150 11 172 8.33e-13 67.0
MS.gene33156.t1 AT3G57040 28.395 162 94 2 11 150 11 172 8.33e-13 67.0
MS.gene33156.t1 AT5G45580 43.182 88 47 2 181 265 11 98 3.70e-12 65.9
MS.gene33156.t1 AT3G04030 54.545 55 24 1 191 244 45 99 7.20e-12 65.9
MS.gene33156.t1 AT3G56380 31.298 131 76 2 9 125 20 150 7.66e-12 62.8
MS.gene33156.t1 AT3G04030 54.545 55 24 1 191 244 45 99 7.81e-12 65.9
MS.gene33156.t1 AT5G45580 44.737 76 41 1 191 265 7 82 1.07e-11 64.3
MS.gene33156.t1 AT3G04030 51.852 54 26 0 191 244 45 98 1.14e-11 65.1
MS.gene33156.t1 AT1G25550 51.667 60 28 1 190 248 208 267 1.33e-11 64.7
MS.gene33156.t1 AT3G56380 31.538 130 76 2 9 125 20 149 1.52e-11 62.0
MS.gene33156.t1 AT4G13640 39.048 105 54 3 187 281 33 137 2.13e-11 63.9
MS.gene33156.t1 AT4G13640 39.048 105 54 3 187 281 33 137 2.16e-11 63.9
MS.gene33156.t1 AT2G40670 32.331 133 74 2 9 125 29 161 2.40e-11 61.6
MS.gene33156.t1 AT1G13300 47.692 65 33 1 185 248 175 239 2.42e-11 63.9
MS.gene33156.t1 AT3G24120 51.613 62 29 1 187 247 37 98 2.55e-11 63.5
MS.gene33156.t1 AT3G24120 51.613 62 29 1 187 247 37 98 2.78e-11 63.5
MS.gene33156.t1 AT1G49560 45.833 72 38 1 186 256 187 258 4.71e-11 63.2
MS.gene33156.t1 AT2G40670 32.576 132 74 2 9 125 29 160 5.13e-11 60.8
MS.gene33156.t1 AT4G37180 49.180 61 30 1 189 248 208 268 5.18e-11 63.2
MS.gene33156.t1 AT4G37180 49.180 61 30 1 189 248 215 275 6.39e-11 62.8

Find 60 sgRNAs with CRISPR-Local

Find 101 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAGAACATGTCTCAAGATTT+TGG 0.179210 1.3:-8663132 MS.gene33156:CDS
ATTCCTTTCATCACCATCTT+TGG 0.256348 1.3:+8662282 None:intergenic
CCATTTCCACTATCAGAATT+AGG 0.318838 1.3:+8662144 None:intergenic
ATATGTGAGACTGAGAAGTA+TGG 0.327860 1.3:+8661208 None:intergenic
ATGAATGATCAGTTTCCTAT+TGG 0.330551 1.3:-8663182 MS.gene33156:CDS
CCTAATTCTGATAGTGGAAA+TGG 0.330629 1.3:-8662144 MS.gene33156:CDS
TTTAAGCTTCTTGAGCTTGT+TGG 0.375182 1.3:-8662437 MS.gene33156:CDS
AAAGGTGGCCAGCCATCTTC+AGG 0.376644 1.3:-8661654 MS.gene33156:intron
ACATGGTGCGTGTGATTATC+TGG 0.387305 1.3:-8662256 MS.gene33156:CDS
TAAAAGAGGTACAGATCATT+TGG 0.388547 1.3:-8662218 MS.gene33156:CDS
TATGAACATTGCTGATTACT+AGG 0.423694 1.3:+8662477 None:intergenic
TACCATGGTATTGACACCTT+TGG 0.431203 1.3:+8663099 None:intergenic
AGAATGTCACTGGGAAAGAT+TGG 0.434197 1.3:+8661242 None:intergenic
TCATTTGGCAGCATGTAATC+AGG 0.452747 1.3:-8662203 MS.gene33156:CDS
AGAAGAAGCCTCGAACTGTT+TGG 0.455763 1.3:-8661993 MS.gene33156:CDS
TGAGCTTGTTGGACTTGAGA+TGG 0.457732 1.3:-8662426 MS.gene33156:CDS
GCAAGAACTCGCATTCCAAT+AGG 0.458411 1.3:+8663167 None:intergenic
AGCTTAAATCCATCCATGTC+TGG 0.471845 1.3:+8662452 None:intergenic
GTTGTTGCGGTCAATCAATT+AGG 0.476132 1.3:-8661946 MS.gene33156:CDS
GACGCGCCTAATTCTGATAG+TGG 0.477794 1.3:-8662150 MS.gene33156:CDS
GGGAAAGATTCGGCTGGGAC+AGG 0.479650 1.3:-8662123 MS.gene33156:CDS
CTAATTCTGATAGTGGAAAT+GGG 0.482228 1.3:-8662143 MS.gene33156:CDS
TGAAAATCTGACAAGGGAAA+AGG 0.489796 1.3:-8661672 MS.gene33156:CDS
TAGCTAAGGCTGCTTCCATA+TGG 0.490232 1.3:+8661296 None:intergenic
CACTGCTCAAAACGCGATAA+CGG 0.499559 1.3:-8662537 MS.gene33156:CDS
TAGTGGAAATGGGAAAGATT+CGG 0.502507 1.3:-8662133 MS.gene33156:CDS
GTCAATCAATTAGGCATTGA+CGG 0.502566 1.3:-8661937 MS.gene33156:intron
GGGTGGGAAAAGAATGTCAC+TGG 0.506615 1.3:+8661232 None:intergenic
AGCATAAAAGTACCTGAAGA+TGG 0.509866 1.3:+8661642 None:intergenic
ACATCGCAAGTTTGTTGTTG+CGG 0.516543 1.3:-8661959 MS.gene33156:CDS
TATGTGAGACTGAGAAGTAT+GGG 0.518755 1.3:+8661209 None:intergenic
GAAACCTGTTAGACTAAAAG+AGG 0.521944 1.3:-8662232 MS.gene33156:CDS
TTGCATAACTTACTTATGAC+AGG 0.526242 1.3:+8662398 None:intergenic
TGACAGTGAGGAAGAAAATG+AGG 0.529313 1.3:-8662046 MS.gene33156:CDS
GGAAATGGGAAAGATTCGGC+TGG 0.530128 1.3:-8662129 MS.gene33156:CDS
TGCATCTGAACTACTAGCTA+AGG 0.539318 1.3:+8661282 None:intergenic
TATTGGAATGCGAGTTCTTG+CGG 0.540178 1.3:-8663165 MS.gene33156:CDS
TAAAAGTACCTGAAGATGGC+TGG 0.546379 1.3:+8661646 None:intergenic
TCAGTGGACCAAACAGTTCG+AGG 0.556377 1.3:+8661985 None:intergenic
CAATGTTCATATGCCAGACA+TGG 0.556391 1.3:-8662465 MS.gene33156:CDS
AGATTGGCTGCTGAGTTGAT+AGG 0.561182 1.3:+8661258 None:intergenic
TAAGAATGAAGATGACAGTG+AGG 0.570920 1.3:-8662058 MS.gene33156:CDS
GGAAGACAGACAAGACCATA+TGG 0.582020 1.3:-8661311 MS.gene33156:CDS
GTTCATATGCCAGACATGGA+TGG 0.584384 1.3:-8662461 MS.gene33156:CDS
ATGTGAGACTGAGAAGTATG+GGG 0.595182 1.3:+8661210 None:intergenic
TGCGAATGATGATCCAAAGA+TGG 0.615837 1.3:-8662295 MS.gene33156:CDS
GGTCTTGTCTGTCTTCCGCA+CGG 0.622559 1.3:+8661317 None:intergenic
GAAATGGGAAAGATTCGGCT+GGG 0.627002 1.3:-8662128 MS.gene33156:CDS
AAATCTGACAAGGGAAAAGG+TGG 0.629315 1.3:-8661669 MS.gene33156:CDS
ATAACTTACTTATGACAGGT+AGG 0.629417 1.3:+8662402 None:intergenic
GATCCAAAGATGGTGATGAA+AGG 0.637771 1.3:-8662285 MS.gene33156:CDS
TGTGAGACTGAGAAGTATGG+GGG 0.643481 1.3:+8661211 None:intergenic
CTCCAAAGGTGTCAATACCA+TGG 0.650196 1.3:-8663101 MS.gene33156:intron
AAACTTGCGATGTAAATCAG+TGG 0.662856 1.3:+8661969 None:intergenic
GTGAGACTGAGAAGTATGGG+GGG 0.686210 1.3:+8661212 None:intergenic
GAATGTTGAAAATCTGACAA+GGG 0.692411 1.3:-8661678 MS.gene33156:CDS
TGAATGTTGAAAATCTGACA+AGG 0.698854 1.3:-8661679 MS.gene33156:CDS
GGTGGGAAAAGAATGTCACT+GGG 0.701317 1.3:+8661233 None:intergenic
GTGATGAAAGGAATTGAACA+TGG 0.742148 1.3:-8662273 MS.gene33156:CDS
TCTACAAAGAATTAGCCGTG+CGG 0.798557 1.3:-8661332 MS.gene33156:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATGATATTAATTTTAGTTA+TGG - chr1.3:8661792-8661811 MS.gene33156:intron 10.0%
!!! AATTTTATTTGATATTGTTT+AGG - chr1.3:8661565-8661584 MS.gene33156:intron 10.0%
!!! TATTTTGAAATATTAAAGAT+TGG - chr1.3:8661337-8661356 MS.gene33156:CDS 10.0%
!! AAAAAACAAGATTAAAATCA+AGG + chr1.3:8661364-8661383 None:intergenic 15.0%
!! TATCAATAATATAAGATTGA+AGG + chr1.3:8662004-8662023 None:intergenic 15.0%
!!! ATTATTATTTTCAATGAAGT+TGG - chr1.3:8661458-8661477 MS.gene33156:intron 15.0%
!! AAATCTAATGATATATAGCT+AGG - chr1.3:8661392-8661411 MS.gene33156:intron 20.0%
!! AATCTAATGATATATAGCTA+GGG - chr1.3:8661393-8661412 MS.gene33156:intron 20.0%
! ATGAAAAACCATCTAGAAAA+AGG - chr1.3:8662288-8662307 MS.gene33156:CDS 25.0%
! CATGATATTAATCTCAACAT+TGG + chr1.3:8662504-8662523 None:intergenic 25.0%
!! GCTAAACATAAGTATTTTTC+GGG + chr1.3:8662582-8662601 None:intergenic 25.0%
!! GGCTAAACATAAGTATTTTT+CGG + chr1.3:8662583-8662602 None:intergenic 25.0%
!! TAATTGATTTCTACAAGCAT+TGG + chr1.3:8661534-8661553 None:intergenic 25.0%
!!! ATCAGTTCAAGAATCTTTTT+TGG + chr1.3:8662676-8662695 None:intergenic 25.0%
AAAACCATCTAGAAAAAGGA+AGG - chr1.3:8662292-8662311 MS.gene33156:CDS 30.0%
AATCGGAAAAATTCATAGCA+TGG + chr1.3:8662604-8662623 None:intergenic 30.0%
ATAACTTACTTATGACAGGT+AGG + chr1.3:8661975-8661994 None:intergenic 30.0%
ATGAATGATCAGTTTCCTAT+TGG - chr1.3:8661192-8661211 MS.gene33156:CDS 30.0%
GAATGTTGAAAATCTGACAA+GGG - chr1.3:8662696-8662715 MS.gene33156:intron 30.0%
TGAATGTTGAAAATCTGACA+AGG - chr1.3:8662695-8662714 MS.gene33156:intron 30.0%
TGAGAAAAACACTTCAGAAT+CGG + chr1.3:8662621-8662640 None:intergenic 30.0%
TTCTCGATTCTTTCAAAGAT+TGG - chr1.3:8661618-8661637 MS.gene33156:intron 30.0%
TTGCATAACTTACTTATGAC+AGG + chr1.3:8661979-8661998 None:intergenic 30.0%
! ATGATATATAGCTAGGGTTT+TGG - chr1.3:8661399-8661418 MS.gene33156:intron 30.0%
! TAAAAGAGGTACAGATCATT+TGG - chr1.3:8662156-8662175 MS.gene33156:CDS 30.0%
! TAGAACATGTCTCAAGATTT+TGG - chr1.3:8661242-8661261 MS.gene33156:CDS 30.0%
! TATGAACATTGCTGATTACT+AGG + chr1.3:8661900-8661919 None:intergenic 30.0%
! TTATCCTTCCTTTTTCTAGA+TGG + chr1.3:8662299-8662318 None:intergenic 30.0%
!! CTAATTCTGATAGTGGAAAT+GGG - chr1.3:8662231-8662250 MS.gene33156:CDS 30.0%
!!! TTTTTCGGGAAGAATATTTG+AGG + chr1.3:8662568-8662587 None:intergenic 30.0%
AAACTTGCGATGTAAATCAG+TGG + chr1.3:8662408-8662427 None:intergenic 35.0%
ACGTAAGATCACCAAAGATT+AGG + chr1.3:8661694-8661713 None:intergenic 35.0%
AGTTGTTATCTTACTTGCAG+AGG - chr1.3:8662645-8662664 MS.gene33156:intron 35.0%
ATATGTGAGACTGAGAAGTA+TGG + chr1.3:8663169-8663188 None:intergenic 35.0%
ATTCCTTTCATCACCATCTT+TGG + chr1.3:8662095-8662114 None:intergenic 35.0%
CCATTTCCACTATCAGAATT+AGG + chr1.3:8662233-8662252 None:intergenic 35.0%
GAAACCTGTTAGACTAAAAG+AGG - chr1.3:8662142-8662161 MS.gene33156:CDS 35.0%
GTGATGAAAGGAATTGAACA+TGG - chr1.3:8662101-8662120 MS.gene33156:CDS 35.0%
TAAGAATGAAGATGACAGTG+AGG - chr1.3:8662316-8662335 MS.gene33156:intron 35.0%
TAGTGGAAATGGGAAAGATT+CGG - chr1.3:8662241-8662260 MS.gene33156:CDS 35.0%
TGAAAATCTGACAAGGGAAA+AGG - chr1.3:8662702-8662721 MS.gene33156:intron 35.0%
TTTAAGCTTCTTGAGCTTGT+TGG - chr1.3:8661937-8661956 MS.gene33156:intron 35.0%
! TATGTGAGACTGAGAAGTAT+GGG + chr1.3:8663168-8663187 None:intergenic 35.0%
! TCTTTTGTGTGCCTAATCTT+TGG - chr1.3:8661680-8661699 MS.gene33156:CDS 35.0%
! TGTACCTCTTTTAGTCTAAC+AGG + chr1.3:8662149-8662168 None:intergenic 35.0%
!! AGCATAAAAGTACCTGAAGA+TGG + chr1.3:8662735-8662754 None:intergenic 35.0%
!! CCTAATTCTGATAGTGGAAA+TGG - chr1.3:8662230-8662249 MS.gene33156:CDS 35.0%
!! GTCAATCAATTAGGCATTGA+CGG - chr1.3:8662437-8662456 MS.gene33156:CDS 35.0%
!! TTTTGGAGAAACTTCTCCAA+AGG - chr1.3:8661259-8661278 MS.gene33156:CDS 35.0%
AAATCTGACAAGGGAAAAGG+TGG - chr1.3:8662705-8662724 MS.gene33156:intron 40.0%
AATCTGCGCATTGAAGAAAG+AGG + chr1.3:8662473-8662492 None:intergenic 40.0%
AGAATGTCACTGGGAAAGAT+TGG + chr1.3:8663135-8663154 None:intergenic 40.0%
AGCTTAAATCCATCCATGTC+TGG + chr1.3:8661925-8661944 None:intergenic 40.0%
ATCTGCGCATTGAAGAAAGA+GGG + chr1.3:8662472-8662491 None:intergenic 40.0%
ATTAACAGTGTCCTTGTGTC+AGG + chr1.3:8662805-8662824 None:intergenic 40.0%
CAATGTTCATATGCCAGACA+TGG - chr1.3:8661909-8661928 MS.gene33156:intron 40.0%
GTTGTTGCGGTCAATCAATT+AGG - chr1.3:8662428-8662447 MS.gene33156:CDS 40.0%
TATTGGAATGCGAGTTCTTG+CGG - chr1.3:8661209-8661228 MS.gene33156:CDS 40.0%
TCATTTGGCAGCATGTAATC+AGG - chr1.3:8662171-8662190 MS.gene33156:CDS 40.0%
TCTACAAAGAATTAGCCGTG+CGG - chr1.3:8663042-8663061 MS.gene33156:intron 40.0%
TGACACAAGGACACTGTTAA+TGG - chr1.3:8662804-8662823 MS.gene33156:intron 40.0%
TGACAGTGAGGAAGAAAATG+AGG - chr1.3:8662328-8662347 MS.gene33156:intron 40.0%
TGCATCTGAACTACTAGCTA+AGG + chr1.3:8663095-8663114 None:intergenic 40.0%
TGCGAATGATGATCCAAAGA+TGG - chr1.3:8662079-8662098 MS.gene33156:CDS 40.0%
TGTCTTTGTCGTGTTAGTGT+CGG - chr1.3:8662922-8662941 MS.gene33156:intron 40.0%
TGTTGATGTCGTGTTAGTGT+CGG - chr1.3:8662862-8662881 MS.gene33156:intron 40.0%
! ATGTGAGACTGAGAAGTATG+GGG + chr1.3:8663167-8663186 None:intergenic 40.0%
! GATCCAAAGATGGTGATGAA+AGG - chr1.3:8662089-8662108 MS.gene33156:CDS 40.0%
!! ACATCGCAAGTTTGTTGTTG+CGG - chr1.3:8662415-8662434 MS.gene33156:CDS 40.0%
!! GGTGATCTTACGTTGTTTCT+TGG - chr1.3:8661701-8661720 MS.gene33156:CDS 40.0%
!! GTTGATGATGAAGCATACCA+TGG + chr1.3:8661293-8661312 None:intergenic 40.0%
!! TAAAAGTACCTGAAGATGGC+TGG + chr1.3:8662731-8662750 None:intergenic 40.0%
!! TACCATGGTATTGACACCTT+TGG + chr1.3:8661278-8661297 None:intergenic 40.0%
!!! CTAGGGTTTTGGCTAAGTTT+TGG - chr1.3:8661410-8661429 MS.gene33156:intron 40.0%
ACATGGTGCGTGTGATTATC+TGG - chr1.3:8662118-8662137 MS.gene33156:CDS 45.0%
AGAAGAAGCCTCGAACTGTT+TGG - chr1.3:8662381-8662400 MS.gene33156:intron 45.0%
CACTGCTCAAAACGCGATAA+CGG - chr1.3:8661837-8661856 MS.gene33156:intron 45.0%
CCTATAATGAGCCTGACACA+AGG - chr1.3:8662791-8662810 MS.gene33156:intron 45.0%
CCTTGTGTCAGGCTCATTAT+AGG + chr1.3:8662794-8662813 None:intergenic 45.0%
GAAATGGGAAAGATTCGGCT+GGG - chr1.3:8662246-8662265 MS.gene33156:CDS 45.0%
GCAAGAACTCGCATTCCAAT+AGG + chr1.3:8661210-8661229 None:intergenic 45.0%
GGAAGACAGACAAGACCATA+TGG - chr1.3:8663063-8663082 MS.gene33156:intron 45.0%
GGTGGGAAAAGAATGTCACT+GGG + chr1.3:8663144-8663163 None:intergenic 45.0%
GTTCATATGCCAGACATGGA+TGG - chr1.3:8661913-8661932 MS.gene33156:intron 45.0%
TAGCTAAGGCTGCTTCCATA+TGG + chr1.3:8663081-8663100 None:intergenic 45.0%
! TGTGAGACTGAGAAGTATGG+GGG + chr1.3:8663166-8663185 None:intergenic 45.0%
!! AGATTGGCTGCTGAGTTGAT+AGG + chr1.3:8663119-8663138 None:intergenic 45.0%
!! CTCCAAAGGTGTCAATACCA+TGG - chr1.3:8661273-8661292 MS.gene33156:CDS 45.0%
!! TGAGCTTGTTGGACTTGAGA+TGG - chr1.3:8661948-8661967 MS.gene33156:CDS 45.0%
GACGCGCCTAATTCTGATAG+TGG - chr1.3:8662224-8662243 MS.gene33156:CDS 50.0%
GGAAATGGGAAAGATTCGGC+TGG - chr1.3:8662245-8662264 MS.gene33156:CDS 50.0%
GGGTGGGAAAAGAATGTCAC+TGG + chr1.3:8663145-8663164 None:intergenic 50.0%
TCAGTGGACCAAACAGTTCG+AGG + chr1.3:8662392-8662411 None:intergenic 50.0%
! GTGAGACTGAGAAGTATGGG+GGG + chr1.3:8663165-8663184 None:intergenic 50.0%
!! CGTTATCGCGTTTTGAGCAG+TGG + chr1.3:8661839-8661858 None:intergenic 50.0%
AAAGGTGGCCAGCCATCTTC+AGG - chr1.3:8662720-8662739 MS.gene33156:intron 55.0%
! AGACTGAGAAGTATGGGGGG+TGG + chr1.3:8663162-8663181 None:intergenic 55.0%
! GACTGAGAAGTATGGGGGGT+GGG + chr1.3:8663161-8663180 None:intergenic 55.0%
! GGTCTTGTCTGTCTTCCGCA+CGG + chr1.3:8663060-8663079 None:intergenic 55.0%
GGACGCAGAATGCACAGCGT+CGG - chr1.3:8662883-8662902 MS.gene33156:intron 60.0%
GGGAAAGATTCGGCTGGGAC+AGG - chr1.3:8662251-8662270 MS.gene33156:CDS 60.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 8661192 8663204 8661192 ID=MS.gene33156
chr1.3 mRNA 8661192 8663204 8661192 ID=MS.gene33156.t1;Parent=MS.gene33156
chr1.3 exon 8663102 8663204 8663102 ID=MS.gene33156.t1.exon1;Parent=MS.gene33156.t1
chr1.3 CDS 8663102 8663204 8663102 ID=cds.MS.gene33156.t1;Parent=MS.gene33156.t1
chr1.3 exon 8662411 8662563 8662411 ID=MS.gene33156.t1.exon2;Parent=MS.gene33156.t1
chr1.3 CDS 8662411 8662563 8662411 ID=cds.MS.gene33156.t1;Parent=MS.gene33156.t1
chr1.3 exon 8661938 8662324 8661938 ID=MS.gene33156.t1.exon3;Parent=MS.gene33156.t1
chr1.3 CDS 8661938 8662324 8661938 ID=cds.MS.gene33156.t1;Parent=MS.gene33156.t1
chr1.3 exon 8661655 8661731 8661655 ID=MS.gene33156.t1.exon4;Parent=MS.gene33156.t1
chr1.3 CDS 8661655 8661731 8661655 ID=cds.MS.gene33156.t1;Parent=MS.gene33156.t1
chr1.3 exon 8661192 8661368 8661192 ID=MS.gene33156.t1.exon5;Parent=MS.gene33156.t1
chr1.3 CDS 8661192 8661368 8661192 ID=cds.MS.gene33156.t1;Parent=MS.gene33156.t1
Gene Sequence

>MS.gene33156

ATGAATGATCAGTTTCCTATTGGAATGCGAGTTCTTGCGGTTGATGATGATAGAACATGTCTCAAGATTTTGGAGAAACTTCTCCAAAGGTGTCAATACCATGGTATGCTTCATCATCAACATCATATTATTGTTGTTTTTGTTTTATTTTGAAATATTAAAGATTGGTCCTTGATTTTAATCTTGTTTTTTGATGTGACAAATCTAATGATATATAGCTAGGGTTTTGGCTAAGTTTTGGATACATATATTTATGCATATGTTAGATTATTATTTTCAATGAAGTTGGTTCATTTTGAATCAAATATCTATTTTTCAATATTATGTTCAACACTACATCCAATGCTTGTAGAAATCAATTAGATTAATTTTAAATTTTATTTGATATTGTTTAGGTTTAGACTAAATTATATTTGATCGATCACATTCTCGATTCTTTCAAAGATTGGACATGATTTTGCTTTTAAAGTTGCAATTTTTTTTAAATATCTTTTGTGTGCCTAATCTTTGGTGATCTTACGTTGTTTCTTGGATTTCAAAACATTTCGTAGATTTTATGTTCTGATTGTATATGCAGTGTATAATTTTTTTCTCTTTGAGAATGATATTAATTTTAGTTATGGTTTGTTAATTTGTTTCAGTAACCACTGCTCAAAACGCGATAACGGCGTTGAACTTGTTGAGAGAAAACAAAAGCAATTTTGACCTAGTAATCAGCAATGTTCATATGCCAGACATGGATGGATTTAAGCTTCTTGAGCTTGTTGGACTTGAGATGGACCTACCTGTCATAAGTAAGTTATGCAATTCCTTCAATCTTATATTATTGATACATGTATAACAAATTATAGTATTTTGATTGCAAAGTGTTTGATTTCAGTGTTTTCTGCGAATGATGATCCAAAGATGGTGATGAAAGGAATTGAACATGGTGCGTGTGATTATCTGGTGAAACCTGTTAGACTAAAAGAGGTACAGATCATTTGGCAGCATGTAATCAGGAAGAAGAAAACTAGCAAAAGAAGCAATCACGACGCGCCTAATTCTGATAGTGGAAATGGGAAAGATTCGGCTGGGACAGGAAATTCTGATAAGAATGAAAAACCATCTAGAAAAAGGAAGGATAAGAATGAAGATGACAGTGAGGAAGAAAATGAGGATGATTATGACAATGATGATCAAACTGCGCAGAAGAAGCCTCGAACTGTTTGGTCCACTGATTTACATCGCAAGTTTGTTGTTGCGGTCAATCAATTAGGCATTGACGGTACGTTCTTCCCTCTTTCTTCAATGCGCAGATTATAACGATCCAATGTTGAGATTAATATCATGATATAAAAATAAAAATTCTACTTTGTATTATTTGAAGACTTCCTCAAATATTCTTCCCGAAAAATACTTATGTTTAGCCATGCTATGAATTTTTCCGATTCTGAAGTGTTTTTCTCATGATAGTTGTTATCTTACTTGCAGAGGCTGTACCAAAAAAGATTCTTGAACTGATGAATGTTGAAAATCTGACAAGGGAAAAGGTGGCCAGCCATCTTCAGGTACTTTTATGCTTTAGCGTTATAAGTTACGAAGATATAGACACGTAAACCTATAATGAGCCTGACACAAGGACACTGTTAATGGTTTGATTGAATGAAGTAATTGAATTTAATCACATATGTTGATGTCGTGTTAGTGTCGGACGCAGAATGCACAGCGTCGGTGCTACACAAATTATCTGTCTTTGTCGTGTTAGTGTCGGACGCAGAATGCACAGCGTCGGTGCTACACAAATTATCTGTCTTTTCATTCAAGAAAACTTACTGTTTTTTAATTTTTCTGCAGAAGTATAGACTCTATCTACAAAGAATTAGCCGTGCGGAAGACAGACAAGACCATATGGAAGCAGCCTTAGCTAGTAGTTCAGATGCATCCTATCAACTCAGCAGCCAATCTTTCCCAGTGACATTCTTTTCCCACCCCCCATACTTCTCAGTCTCACATATCTTTCATGATTTGTAA
Protein sequence

>MS.gene33156.t1

MNDQFPIGMRVLAVDDDRTCLKILEKLLQRCQYHVTTAQNAITALNLLRENKSNFDLVISNVHMPDMDGFKLLELVGLEMDLPVIMFSANDDPKMVMKGIEHGACDYLVKPVRLKEVQIIWQHVIRKKKTSKRSNHDAPNSDSGNGKDSAGTGNSDKNEKPSRKRKDKNEDDSEEENEDDYDNDDQTAQKKPRTVWSTDLHRKFVVAVNQLGIDEAVPKKILELMNVENLTREKVASHLQKYRLYLQRISRAEDRQDHMEAALASSSDASYQLSSQSFPVTFFSHPPYFSVSHIFHDL