AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene33157

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene33157.t1 MTR_1g013170 96.525 259 9 0 15 273 1 259 1.98e-170 482
MS.gene33157.t1 MTR_1g013180 78.214 280 54 1 6 285 143 415 5.89e-142 416
MS.gene33157.t1 MTR_7g117705 80.524 267 50 2 15 280 1 266 6.58e-142 408
MS.gene33157.t1 MTR_3g106220 69.091 275 78 3 16 285 5 277 9.99e-123 365
MS.gene33157.t1 MTR_2g450070 58.696 276 102 3 17 283 16 288 3.79e-98 303
MS.gene33157.t1 MTR_2g450070 58.696 276 102 3 17 283 16 288 7.90e-98 303
MS.gene33157.t1 MTR_4g121020 53.357 283 99 4 17 285 29 292 2.84e-94 293
MS.gene33157.t1 MTR_4g121020 53.357 283 99 4 17 285 29 292 2.97e-94 293
MS.gene33157.t1 MTR_2g034960 49.650 286 120 2 14 289 24 295 1.15e-92 287
MS.gene33157.t1 MTR_1g032570 53.846 260 115 2 18 273 10 268 9.00e-91 283
MS.gene33157.t1 MTR_4g131580 57.143 259 104 3 19 277 17 268 1.12e-88 277
MS.gene33157.t1 MTR_4g131600 54.945 273 114 5 1 271 1 266 1.34e-88 276
MS.gene33157.t1 MTR_8g079940 56.863 255 106 2 17 270 23 274 5.42e-88 273
MS.gene33157.t1 MTR_1g013160 93.243 148 10 0 81 228 1 148 5.59e-86 255
MS.gene33157.t1 MTR_4g131570 52.985 268 124 2 19 285 17 283 7.44e-86 269
MS.gene33157.t1 MTR_3g086100 51.254 279 127 3 19 293 13 286 2.29e-84 265
MS.gene33157.t1 MTR_3g102590 51.292 271 75 4 16 283 5 221 9.64e-76 232
MS.gene33157.t1 MTR_3g102590 53.750 240 58 2 16 254 5 192 7.85e-71 218
MS.gene33157.t1 MTR_1g032570 53.000 200 89 2 78 273 1 199 6.76e-64 211
MS.gene33157.t1 MTR_3g102600 47.177 248 69 3 16 263 5 190 1.63e-59 190
MS.gene33157.t1 MTR_3g086100 48.636 220 104 3 78 293 1 215 4.71e-58 194
MS.gene33157.t1 MTR_4g021760 38.406 276 139 5 19 293 11 256 1.19e-56 186
MS.gene33157.t1 MTR_3g102600 46.058 241 68 3 16 256 5 183 1.52e-55 179
MS.gene33157.t1 MTR_4g021790 37.363 273 142 5 13 285 4 247 7.22e-51 169
MS.gene33157.t1 MTR_4g021855 35.897 273 146 4 13 285 4 247 1.68e-50 169
MS.gene33157.t1 MTR_4g021845 38.367 245 123 4 10 254 1 217 3.29e-49 163
MS.gene33157.t1 MTR_4g121020 44.554 202 79 4 98 285 6 188 3.06e-47 167
MS.gene33157.t1 MTR_0450s0040 37.344 241 130 3 23 263 8 227 2.17e-44 155
MS.gene33157.t1 MTR_8g105600 35.689 283 153 7 11 285 1 262 7.76e-41 150
MS.gene33157.t1 MTR_3g102600 41.872 203 56 3 61 263 1 141 2.97e-39 136
MS.gene33157.t1 MTR_6g045327 33.074 257 142 3 6 261 1 228 7.85e-38 138
MS.gene33157.t1 MTR_7g026400 34.855 241 127 3 46 279 14 231 4.21e-37 139
MS.gene33157.t1 MTR_3g102600 40.306 196 55 3 61 256 1 134 2.03e-35 125
MS.gene33157.t1 MTR_5g055260 33.202 253 114 7 10 262 1 198 4.37e-33 122
MS.gene33157.t1 MTR_8g032710 30.040 253 159 5 22 257 20 271 1.25e-28 115
MS.gene33157.t1 MTR_6g016850 36.842 133 81 2 11 141 1 132 1.76e-24 97.8
MS.gene33157.t1 MTR_4g064730 47.788 113 53 2 170 276 94 206 4.94e-23 97.1
MS.gene33157.t1 MTR_3g092780 34.532 139 86 2 23 157 56 193 8.75e-23 99.0
MS.gene33157.t1 MTR_3g092780 34.532 139 86 2 23 157 56 193 9.88e-23 99.0
MS.gene33157.t1 MTR_4g023980 32.530 166 85 3 66 231 36 174 1.19e-22 93.2
MS.gene33157.t1 MTR_8g024260 34.524 168 96 3 25 178 38 205 3.41e-22 97.4
MS.gene33157.t1 MTR_3g092780 37.008 127 75 2 23 145 56 181 7.59e-22 96.3
MS.gene33157.t1 MTR_3g092780 37.008 127 75 2 23 145 56 181 8.97e-22 96.3
MS.gene33157.t1 MTR_7g118260 35.484 155 85 4 16 158 5 156 3.89e-21 94.0
MS.gene33157.t1 MTR_7g118260 30.366 191 118 4 16 194 5 192 2.77e-20 91.7
MS.gene33157.t1 MTR_7g089010 53.846 78 36 0 186 263 72 149 3.60e-20 89.0
MS.gene33157.t1 MTR_8g093040 29.098 244 130 4 18 261 13 213 1.37e-19 87.0
MS.gene33157.t1 MTR_1g067110 36.885 122 74 2 23 141 85 206 1.54e-19 89.4
MS.gene33157.t1 MTR_1g067110 36.885 122 74 2 23 141 85 206 1.60e-19 89.4
MS.gene33157.t1 MTR_4g061360 36.885 122 74 1 23 141 89 210 2.63e-19 89.0
MS.gene33157.t1 MTR_6g453220 77.358 53 12 0 221 273 379 431 2.79e-19 88.6
MS.gene33157.t1 MTR_3g037390 33.871 124 79 1 23 143 24 147 2.07e-18 85.9
MS.gene33157.t1 MTR_5g014040 52.703 74 34 1 198 270 304 377 3.15e-17 82.4
MS.gene33157.t1 MTR_5g014040 35.200 125 75 3 19 141 14 134 3.20e-12 67.4
MS.gene33157.t1 MTR_4g108880 32.258 124 81 1 23 143 33 156 7.71e-17 81.3
MS.gene33157.t1 MTR_4g098870 55.072 69 30 1 203 270 308 376 2.44e-16 79.7
MS.gene33157.t1 MTR_4g098870 32.258 124 80 2 19 141 14 134 5.92e-11 63.5
MS.gene33157.t1 MTR_4g098870 55.072 69 30 1 203 270 308 376 2.48e-16 79.7
MS.gene33157.t1 MTR_4g098870 32.258 124 80 2 19 141 14 134 7.13e-11 63.2
MS.gene33157.t1 MTR_4g098870 55.072 69 30 1 203 270 347 415 2.67e-16 79.7
MS.gene33157.t1 MTR_4g098870 32.258 124 80 2 19 141 53 173 7.62e-11 63.2
MS.gene33157.t1 MTR_4g098870 55.072 69 30 1 203 270 328 396 2.74e-16 79.7
MS.gene33157.t1 MTR_4g098870 32.258 124 80 2 19 141 34 154 6.37e-11 63.5
MS.gene33157.t1 MTR_4g098870 55.072 69 30 1 203 270 328 396 2.83e-16 79.7
MS.gene33157.t1 MTR_4g098870 32.258 124 80 2 19 141 34 154 7.26e-11 63.2
MS.gene33157.t1 MTR_5g014040 49.367 79 34 2 198 270 264 342 3.08e-15 76.3
MS.gene33157.t1 MTR_4g078810 55.357 56 25 0 202 257 155 210 3.59e-15 75.9
MS.gene33157.t1 MTR_5g014040 49.367 79 34 2 198 270 304 382 3.77e-15 76.3
MS.gene33157.t1 MTR_5g014040 35.200 125 75 3 19 141 14 134 3.27e-12 67.4
MS.gene33157.t1 MTR_4g086835 50.746 67 33 0 200 266 269 335 6.53e-15 75.1
MS.gene33157.t1 MTR_2g090675 55.357 56 25 0 202 257 158 213 9.21e-15 74.7
MS.gene33157.t1 MTR_1g093080 41.379 87 50 1 191 276 197 283 2.72e-14 73.2
MS.gene33157.t1 MTR_4g113140 52.857 70 31 2 196 264 227 295 5.37e-14 72.4
MS.gene33157.t1 MTR_5g017980 51.613 62 30 0 202 263 190 251 7.66e-14 71.2
MS.gene33157.t1 MTR_5g017980 51.613 62 30 0 202 263 189 250 8.05e-14 71.2
MS.gene33157.t1 MTR_7g115530 64.286 56 19 1 203 257 216 271 1.36e-13 70.5
MS.gene33157.t1 MTR_7g115530 54.286 70 31 1 203 271 216 285 1.69e-13 70.5
MS.gene33157.t1 MTR_7g115530 64.286 56 19 1 203 257 216 271 2.05e-13 70.1
MS.gene33157.t1 MTR_4g086835 50.000 68 33 1 200 266 269 336 2.38e-13 70.5
MS.gene33157.t1 MTR_1g080330 60.345 58 22 1 201 257 171 228 2.97e-13 69.3
MS.gene33157.t1 MTR_7g115530 64.286 56 19 1 203 257 216 271 3.33e-13 70.1
MS.gene33157.t1 MTR_1g080330 60.345 58 22 1 201 257 171 228 4.18e-13 69.7
MS.gene33157.t1 MTR_7g098250 52.941 68 31 1 201 267 258 325 1.02e-12 68.9
MS.gene33157.t1 MTR_2g023580 47.826 92 40 4 204 288 54 144 1.16e-12 67.0
MS.gene33157.t1 MTR_5g054300 43.590 78 43 1 186 262 179 256 1.21e-12 68.2
MS.gene33157.t1 MTR_5g017980 50.794 63 30 1 202 263 190 252 1.54e-12 67.4
MS.gene33157.t1 MTR_3g093860 34.507 142 69 6 13 136 11 146 2.49e-12 64.3
MS.gene33157.t1 MTR_1g090670 54.839 62 27 1 201 261 40 101 2.75e-12 66.6
MS.gene33157.t1 MTR_6g088950 30.645 124 85 1 21 143 5 128 3.57e-12 65.1
MS.gene33157.t1 MTR_7g115530 62.963 54 19 1 203 255 216 269 7.85e-12 65.1
MS.gene33157.t1 MTR_6g032990 54.545 55 24 1 205 258 42 96 9.05e-12 65.5
MS.gene33157.t1 MTR_2g086450 54.545 55 24 1 205 258 45 99 9.87e-12 65.5
MS.gene33157.t1 MTR_7g490310 30.216 139 76 3 25 142 11 149 1.14e-11 63.9
MS.gene33157.t1 MTR_8g038620 28.986 138 78 3 25 142 11 148 1.72e-11 62.4
MS.gene33157.t1 MTR_5g027440 56.364 55 23 1 205 258 25 79 1.73e-11 63.9
MS.gene33157.t1 MTR_5g041350 50.909 55 26 1 205 258 15 69 6.48e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene33157.t1 AT2G25180 63.953 258 87 3 17 273 11 263 3.83e-106 322
MS.gene33157.t1 AT4G31920 58.803 284 95 5 17 299 11 273 2.28e-102 311
MS.gene33157.t1 AT3G16857 49.477 287 127 2 17 285 31 317 1.20e-90 283
MS.gene33157.t1 AT3G16857 49.477 287 127 2 17 285 31 317 2.72e-90 283
MS.gene33157.t1 AT5G58080 55.396 278 98 5 17 285 12 272 1.47e-83 264
MS.gene33157.t1 AT1G67710 54.150 253 109 3 20 268 8 257 8.79e-80 251
MS.gene33157.t1 AT2G01760 47.138 297 138 5 15 296 3 295 2.58e-78 243
MS.gene33157.t1 AT3G16857 48.780 246 108 2 58 285 2 247 2.01e-73 238
MS.gene33157.t1 AT2G01760 43.077 325 138 6 15 296 3 323 1.40e-72 229
MS.gene33157.t1 AT1G67710 52.308 195 86 3 78 268 1 192 3.04e-53 181
MS.gene33157.t1 AT5G49240 38.430 242 146 2 14 254 33 272 2.51e-51 171
MS.gene33157.t1 AT2G01760 43.348 233 122 4 78 300 1 233 1.40e-49 167
MS.gene33157.t1 AT3G62670 34.628 309 159 9 24 306 42 333 2.39e-46 162
MS.gene33157.t1 AT1G67710 46.857 175 86 3 98 268 12 183 1.80e-38 141
MS.gene33157.t1 AT1G49190 31.206 282 175 4 18 288 29 302 4.83e-35 131
MS.gene33157.t1 AT5G07210 31.047 277 149 7 21 264 14 281 8.64e-33 127
MS.gene33157.t1 AT5G07210 30.175 285 149 8 21 264 14 289 3.16e-30 120
MS.gene33157.t1 AT2G27070 29.577 284 145 7 21 263 14 283 5.27e-28 114
MS.gene33157.t1 AT2G46790 39.837 123 69 2 23 141 37 158 1.59e-24 103
MS.gene33157.t1 AT2G46790 39.837 123 69 2 23 141 37 158 1.73e-24 103
MS.gene33157.t1 AT5G59570 48.214 112 50 2 184 292 120 226 1.38e-23 98.6
MS.gene33157.t1 AT5G59570 48.214 112 50 2 184 292 120 226 1.38e-23 98.6
MS.gene33157.t1 AT1G49190 37.600 125 78 0 18 142 29 153 2.46e-23 100
MS.gene33157.t1 AT2G40970 41.525 118 69 0 181 298 81 198 2.51e-23 96.7
MS.gene33157.t1 AT3G46640 36.420 162 83 4 118 263 45 202 5.06e-22 94.7
MS.gene33157.t1 AT5G24470 36.719 128 76 2 21 144 48 174 5.11e-22 96.7
MS.gene33157.t1 AT3G46640 37.037 162 82 5 118 263 45 202 8.06e-22 94.0
MS.gene33157.t1 AT3G46640 37.037 162 82 5 118 263 45 202 8.06e-22 94.0
MS.gene33157.t1 AT5G02810 38.525 122 72 1 23 141 78 199 9.85e-21 93.2
MS.gene33157.t1 AT5G05090 53.165 79 37 0 185 263 61 139 1.23e-20 89.7
MS.gene33157.t1 AT1G68210 25.632 277 177 3 23 271 13 288 1.98e-20 92.0
MS.gene33157.t1 AT3G10760 65.000 60 21 0 204 263 104 163 3.40e-20 89.7
MS.gene33157.t1 AT5G60100 37.795 127 75 2 19 141 59 185 3.86e-20 90.5
MS.gene33157.t1 AT5G60100 37.795 127 75 2 19 141 59 185 3.86e-20 90.5
MS.gene33157.t1 AT5G60100 37.795 127 75 2 19 141 59 185 3.86e-20 90.5
MS.gene33157.t1 AT5G60100 37.795 127 75 2 19 141 59 185 5.59e-20 90.5
MS.gene33157.t1 AT5G60100 37.795 127 75 2 19 141 59 185 5.59e-20 90.5
MS.gene33157.t1 AT5G60100 37.795 127 75 2 19 141 59 185 5.59e-20 90.5
MS.gene33157.t1 AT5G60100 37.795 127 75 2 19 141 59 185 7.31e-20 90.1
MS.gene33157.t1 AT5G44190 40.678 118 64 2 197 310 139 254 4.39e-19 87.0
MS.gene33157.t1 AT5G44190 40.678 118 64 2 197 310 139 254 5.20e-19 86.7
MS.gene33157.t1 AT5G61380 33.871 124 79 1 23 143 19 142 1.62e-18 86.3
MS.gene33157.t1 AT2G20570 44.330 97 43 1 167 263 126 211 2.06e-16 79.7
MS.gene33157.t1 AT2G20570 61.111 54 21 0 210 263 174 227 4.62e-15 75.9
MS.gene33157.t1 AT4G18020 51.429 70 32 1 199 268 292 359 1.26e-14 74.7
MS.gene33157.t1 AT4G18020 51.429 70 32 1 199 268 292 359 1.26e-14 74.7
MS.gene33157.t1 AT4G18020 51.429 70 32 1 199 268 292 359 1.26e-14 74.7
MS.gene33157.t1 AT4G18020 51.429 70 32 1 199 268 292 359 1.26e-14 74.7
MS.gene33157.t1 AT4G18020 51.429 70 32 1 199 268 292 359 1.26e-14 74.7
MS.gene33157.t1 AT4G18020 51.429 70 32 1 199 268 292 359 1.26e-14 74.7
MS.gene33157.t1 AT4G18020 51.429 70 32 1 199 268 292 359 1.51e-14 74.3
MS.gene33157.t1 AT4G18020 51.429 70 32 1 199 268 292 359 1.51e-14 74.3
MS.gene33157.t1 AT4G18020 51.429 70 32 1 199 268 292 359 1.51e-14 74.3
MS.gene33157.t1 AT4G18020 51.429 70 32 1 199 268 292 359 1.51e-14 74.3
MS.gene33157.t1 AT4G18020 51.429 70 32 1 199 268 292 359 1.51e-14 74.3
MS.gene33157.t1 AT2G03500 53.125 64 29 1 202 264 230 293 2.32e-13 70.5
MS.gene33157.t1 AT3G57040 28.395 162 94 2 25 164 11 172 1.21e-12 66.6
MS.gene33157.t1 AT3G57040 28.395 162 94 2 25 164 11 172 1.21e-12 66.6
MS.gene33157.t1 AT5G45580 43.182 88 47 2 195 279 11 98 3.21e-12 66.2
MS.gene33157.t1 AT3G56380 31.298 131 76 2 23 139 20 150 7.79e-12 62.8
MS.gene33157.t1 AT3G04030 54.545 55 24 1 205 258 45 99 8.57e-12 65.9
MS.gene33157.t1 AT3G04030 54.545 55 24 1 205 258 45 99 9.22e-12 65.9
MS.gene33157.t1 AT5G45580 44.737 76 41 1 205 279 7 82 9.47e-12 64.7
MS.gene33157.t1 AT1G25550 45.070 71 37 2 194 262 197 267 1.14e-11 65.1
MS.gene33157.t1 AT3G04030 51.852 54 26 0 205 258 45 98 1.47e-11 65.1
MS.gene33157.t1 AT3G56380 31.538 130 76 2 23 139 20 149 1.60e-11 62.0
MS.gene33157.t1 AT4G13640 39.048 105 54 3 201 295 33 137 2.07e-11 63.9
MS.gene33157.t1 AT1G13300 47.692 65 33 1 199 262 175 239 2.10e-11 64.3
MS.gene33157.t1 AT2G40670 32.331 133 74 2 23 139 29 161 2.14e-11 62.0
MS.gene33157.t1 AT4G13640 39.048 105 54 3 201 295 33 137 2.35e-11 63.9
MS.gene33157.t1 AT3G24120 51.613 62 29 1 201 261 37 98 2.63e-11 63.5
MS.gene33157.t1 AT3G24120 51.613 62 29 1 201 261 37 98 2.71e-11 63.5
MS.gene33157.t1 AT1G49560 45.833 72 38 1 200 270 187 258 3.25e-11 63.5
MS.gene33157.t1 AT2G40670 32.576 132 74 2 23 139 29 160 4.14e-11 61.2
MS.gene33157.t1 AT4G37180 49.180 61 30 1 203 262 208 268 4.25e-11 63.5
MS.gene33157.t1 AT4G37180 49.180 61 30 1 203 262 215 275 5.34e-11 63.2
MS.gene33157.t1 AT1G68670 41.558 77 44 1 187 262 197 273 9.40e-11 62.4

Find 64 sgRNAs with CRISPR-Local

Find 107 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAGAACATGTCTCAAGATTT+TGG 0.179210 1.3:-8668928 MS.gene33157:CDS
ATTCCTTTCATCACCATCTT+TGG 0.256348 1.3:+8668078 None:intergenic
CCATTTCCACTATCAGAATT+AGG 0.318838 1.3:+8667940 None:intergenic
ATATGTGAGACTGAGAAGTA+TGG 0.327860 1.3:+8667004 None:intergenic
ATGAATGATCAGTTTCCTAT+TGG 0.330551 1.3:-8668978 MS.gene33157:CDS
CCTAATTCTGATAGTGGAAA+TGG 0.330629 1.3:-8667940 MS.gene33157:CDS
ACTCTCCATAGTAACTTTCA+TGG 0.356998 1.3:-8669118 MS.gene33157:intron
TTTAAGCTTCTTGAGCTTGT+TGG 0.375182 1.3:-8668233 MS.gene33157:CDS
AAAGGTGGCCAGCCATCTTC+AGG 0.376644 1.3:-8667450 MS.gene33157:intron
ACATGGTGCGTGTGATTATC+TGG 0.387305 1.3:-8668052 MS.gene33157:CDS
TAAAAGAGGTACAGATCATT+TGG 0.388547 1.3:-8668014 MS.gene33157:CDS
AGGTACCATGAAAGTTACTA+TGG 0.409840 1.3:+8669113 None:intergenic
TATGAACATTGCTGATTACT+AGG 0.423694 1.3:+8668273 None:intergenic
TACCATGGTATTGACACCTT+TGG 0.431203 1.3:+8668895 None:intergenic
AGAATGTCACTGGGAAAGAT+TGG 0.434197 1.3:+8667038 None:intergenic
TCATTTGGCAGCATGTAATC+AGG 0.452747 1.3:-8667999 MS.gene33157:CDS
AGAAGAAGCCTCGAACTGTT+TGG 0.455763 1.3:-8667789 MS.gene33157:CDS
TGAGCTTGTTGGACTTGAGA+TGG 0.457732 1.3:-8668222 MS.gene33157:CDS
GCAAGAACTCGCATTCCAAT+AGG 0.458411 1.3:+8668963 None:intergenic
AGCTTAAATCCATCCATGTC+TGG 0.471845 1.3:+8668248 None:intergenic
GTTGTTGCGGTCAATCAATT+AGG 0.476132 1.3:-8667742 MS.gene33157:CDS
GACGCGCCTAATTCTGATAG+TGG 0.477794 1.3:-8667946 MS.gene33157:CDS
GGGAAAGATTCGGCTGGGAC+AGG 0.479650 1.3:-8667919 MS.gene33157:CDS
CTAATTCTGATAGTGGAAAT+GGG 0.482228 1.3:-8667939 MS.gene33157:CDS
TGAAAATCTGACAAGGGAAA+AGG 0.489796 1.3:-8667468 MS.gene33157:CDS
TAGCTAAGGCTGCTTCCATA+TGG 0.490232 1.3:+8667092 None:intergenic
CACTGCTCAAAACGCGATAA+CGG 0.499559 1.3:-8668333 MS.gene33157:CDS
TAGTGGAAATGGGAAAGATT+CGG 0.502507 1.3:-8667929 MS.gene33157:CDS
GTCAATCAATTAGGCATTGA+CGG 0.502566 1.3:-8667733 MS.gene33157:intron
GGGTGGGAAAAGAATGTCAC+TGG 0.506615 1.3:+8667028 None:intergenic
AGCATAAAAGTACCTGAAGA+TGG 0.509866 1.3:+8667438 None:intergenic
ACATCGCAAGTTTGTTGTTG+CGG 0.516543 1.3:-8667755 MS.gene33157:CDS
TATGTGAGACTGAGAAGTAT+GGG 0.518755 1.3:+8667005 None:intergenic
GAAACCTGTTAGACTAAAAG+AGG 0.521944 1.3:-8668028 MS.gene33157:CDS
TTGCATAACTTACTTATGAC+AGG 0.526242 1.3:+8668194 None:intergenic
TGACAGTGAGGAAGAAAATG+AGG 0.529313 1.3:-8667842 MS.gene33157:CDS
GGAAATGGGAAAGATTCGGC+TGG 0.530128 1.3:-8667925 MS.gene33157:CDS
TGCATCTGAACTACTAGCTA+AGG 0.539318 1.3:+8667078 None:intergenic
TATTGGAATGCGAGTTCTTG+CGG 0.540178 1.3:-8668961 MS.gene33157:CDS
TAAAAGTACCTGAAGATGGC+TGG 0.546379 1.3:+8667442 None:intergenic
TCAGTGGACCAAACAGTTCG+AGG 0.556377 1.3:+8667781 None:intergenic
CAATGTTCATATGCCAGACA+TGG 0.556391 1.3:-8668261 MS.gene33157:CDS
TGAAACAATGTAGAGGATCA+TGG 0.558189 1.3:+8669140 None:intergenic
AGATTGGCTGCTGAGTTGAT+AGG 0.561182 1.3:+8667054 None:intergenic
TAAGAATGAAGATGACAGTG+AGG 0.570920 1.3:-8667854 MS.gene33157:CDS
GGAAGACAGACAAGACCATA+TGG 0.582020 1.3:-8667107 MS.gene33157:CDS
GTTCATATGCCAGACATGGA+TGG 0.584384 1.3:-8668257 MS.gene33157:CDS
ATGTGAGACTGAGAAGTATG+GGG 0.595182 1.3:+8667006 None:intergenic
TGCGAATGATGATCCAAAGA+TGG 0.615837 1.3:-8668091 MS.gene33157:CDS
GGTCTTGTCTGTCTTCCGCA+CGG 0.622559 1.3:+8667113 None:intergenic
GAAATGGGAAAGATTCGGCT+GGG 0.627002 1.3:-8667924 MS.gene33157:CDS
AAATCTGACAAGGGAAAAGG+TGG 0.629315 1.3:-8667465 MS.gene33157:CDS
ATAACTTACTTATGACAGGT+AGG 0.629417 1.3:+8668198 None:intergenic
GATCCAAAGATGGTGATGAA+AGG 0.637771 1.3:-8668081 MS.gene33157:CDS
TGTGAGACTGAGAAGTATGG+GGG 0.643481 1.3:+8667007 None:intergenic
CTCCAAAGGTGTCAATACCA+TGG 0.650196 1.3:-8668897 MS.gene33157:intron
AAACTTGCGATGTAAATCAG+TGG 0.662856 1.3:+8667765 None:intergenic
TGGAGAGTGAAACAATGTAG+AGG 0.667904 1.3:+8669133 None:intergenic
GTGAGACTGAGAAGTATGGG+GGG 0.686210 1.3:+8667008 None:intergenic
GAATGTTGAAAATCTGACAA+GGG 0.692411 1.3:-8667474 MS.gene33157:CDS
TGAATGTTGAAAATCTGACA+AGG 0.698854 1.3:-8667475 MS.gene33157:CDS
GGTGGGAAAAGAATGTCACT+GGG 0.701317 1.3:+8667029 None:intergenic
GTGATGAAAGGAATTGAACA+TGG 0.742148 1.3:-8668069 MS.gene33157:CDS
TCTACAAAGAATTAGCCGTG+CGG 0.798557 1.3:-8667128 MS.gene33157:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATGATATTAATTTTAGTTA+TGG - chr1.3:8667748-8667767 MS.gene33157:CDS 10.0%
!!! AATTTTATTTGATATTGTTT+AGG - chr1.3:8667521-8667540 MS.gene33157:intron 10.0%
!!! TATTTTGAAATATTAAAGAT+TGG - chr1.3:8667293-8667312 MS.gene33157:intron 10.0%
!! AAAAAACAAGATTAAAATCA+AGG + chr1.3:8667320-8667339 None:intergenic 15.0%
!! TATCAATAATATAAGATTGA+AGG + chr1.3:8667960-8667979 None:intergenic 15.0%
!!! ATTATTATTTTCAATGAAGT+TGG - chr1.3:8667414-8667433 MS.gene33157:intron 15.0%
!! AAATCTAATGATATATAGCT+AGG - chr1.3:8667348-8667367 MS.gene33157:intron 20.0%
!! AAGTATATATGGAAAAAAGA+AGG + chr1.3:8667036-8667055 None:intergenic 20.0%
!! AATCTAATGATATATAGCTA+GGG - chr1.3:8667349-8667368 MS.gene33157:intron 20.0%
!! AGAATGTTGTAAAGTATATA+TGG + chr1.3:8667047-8667066 None:intergenic 20.0%
! ATGAAAAACCATCTAGAAAA+AGG - chr1.3:8668244-8668263 MS.gene33157:CDS 25.0%
! CATGATATTAATCTCAACAT+TGG + chr1.3:8668460-8668479 None:intergenic 25.0%
!! CATTCATCTTGTGATTTTTA+AGG + chr1.3:8667135-8667154 None:intergenic 25.0%
!! GCTAAACATAAGTATTTTTC+GGG + chr1.3:8668538-8668557 None:intergenic 25.0%
!! GGCTAAACATAAGTATTTTT+CGG + chr1.3:8668539-8668558 None:intergenic 25.0%
!! TAATTGATTTCTACAAGCAT+TGG + chr1.3:8667490-8667509 None:intergenic 25.0%
!!! ATCAGTTCAAGAATCTTTTT+TGG + chr1.3:8668632-8668651 None:intergenic 25.0%
AAAACCATCTAGAAAAAGGA+AGG - chr1.3:8668248-8668267 MS.gene33157:CDS 30.0%
AATCGGAAAAATTCATAGCA+TGG + chr1.3:8668560-8668579 None:intergenic 30.0%
ATAACTTACTTATGACAGGT+AGG + chr1.3:8667931-8667950 None:intergenic 30.0%
ATGAATGATCAGTTTCCTAT+TGG - chr1.3:8667148-8667167 MS.gene33157:intron 30.0%
GAATGTTGAAAATCTGACAA+GGG - chr1.3:8668652-8668671 MS.gene33157:intron 30.0%
TGAATGTTGAAAATCTGACA+AGG - chr1.3:8668651-8668670 MS.gene33157:intron 30.0%
TGAGAAAAACACTTCAGAAT+CGG + chr1.3:8668577-8668596 None:intergenic 30.0%
TTCTCGATTCTTTCAAAGAT+TGG - chr1.3:8667574-8667593 MS.gene33157:intron 30.0%
TTGCATAACTTACTTATGAC+AGG + chr1.3:8667935-8667954 None:intergenic 30.0%
! ATGATATATAGCTAGGGTTT+TGG - chr1.3:8667355-8667374 MS.gene33157:intron 30.0%
! TAAAAGAGGTACAGATCATT+TGG - chr1.3:8668112-8668131 MS.gene33157:intron 30.0%
! TAGAACATGTCTCAAGATTT+TGG - chr1.3:8667198-8667217 MS.gene33157:intron 30.0%
! TATGAACATTGCTGATTACT+AGG + chr1.3:8667856-8667875 None:intergenic 30.0%
! TTATCCTTCCTTTTTCTAGA+TGG + chr1.3:8668255-8668274 None:intergenic 30.0%
!! CTAATTCTGATAGTGGAAAT+GGG - chr1.3:8668187-8668206 MS.gene33157:intron 30.0%
!!! TTTTTCGGGAAGAATATTTG+AGG + chr1.3:8668524-8668543 None:intergenic 30.0%
AAACTTGCGATGTAAATCAG+TGG + chr1.3:8668364-8668383 None:intergenic 35.0%
ACGTAAGATCACCAAAGATT+AGG + chr1.3:8667650-8667669 None:intergenic 35.0%
ACTCTCCATAGTAACTTTCA+TGG - chr1.3:8667008-8667027 MS.gene33157:CDS 35.0%
AGGTACCATGAAAGTTACTA+TGG + chr1.3:8667016-8667035 None:intergenic 35.0%
AGTTGTTATCTTACTTGCAG+AGG - chr1.3:8668601-8668620 MS.gene33157:intron 35.0%
ATATGTGAGACTGAGAAGTA+TGG + chr1.3:8669125-8669144 None:intergenic 35.0%
ATTCCTTTCATCACCATCTT+TGG + chr1.3:8668051-8668070 None:intergenic 35.0%
CCATTTCCACTATCAGAATT+AGG + chr1.3:8668189-8668208 None:intergenic 35.0%
GAAACCTGTTAGACTAAAAG+AGG - chr1.3:8668098-8668117 MS.gene33157:CDS 35.0%
GTGATGAAAGGAATTGAACA+TGG - chr1.3:8668057-8668076 MS.gene33157:CDS 35.0%
TAAGAATGAAGATGACAGTG+AGG - chr1.3:8668272-8668291 MS.gene33157:CDS 35.0%
TAGTGGAAATGGGAAAGATT+CGG - chr1.3:8668197-8668216 MS.gene33157:intron 35.0%
TGAAAATCTGACAAGGGAAA+AGG - chr1.3:8668658-8668677 MS.gene33157:intron 35.0%
TTTAAGCTTCTTGAGCTTGT+TGG - chr1.3:8667893-8667912 MS.gene33157:CDS 35.0%
! TATGTGAGACTGAGAAGTAT+GGG + chr1.3:8669124-8669143 None:intergenic 35.0%
! TCTTTTGTGTGCCTAATCTT+TGG - chr1.3:8667636-8667655 MS.gene33157:intron 35.0%
! TGTACCTCTTTTAGTCTAAC+AGG + chr1.3:8668105-8668124 None:intergenic 35.0%
!! AGCATAAAAGTACCTGAAGA+TGG + chr1.3:8668691-8668710 None:intergenic 35.0%
!! CCTAATTCTGATAGTGGAAA+TGG - chr1.3:8668186-8668205 MS.gene33157:intron 35.0%
!! GTCAATCAATTAGGCATTGA+CGG - chr1.3:8668393-8668412 MS.gene33157:intron 35.0%
!! TTTTGGAGAAACTTCTCCAA+AGG - chr1.3:8667215-8667234 MS.gene33157:intron 35.0%
AAATCTGACAAGGGAAAAGG+TGG - chr1.3:8668661-8668680 MS.gene33157:intron 40.0%
AATCTGCGCATTGAAGAAAG+AGG + chr1.3:8668429-8668448 None:intergenic 40.0%
AGAATGTCACTGGGAAAGAT+TGG + chr1.3:8669091-8669110 None:intergenic 40.0%
AGCTTAAATCCATCCATGTC+TGG + chr1.3:8667881-8667900 None:intergenic 40.0%
ATCTGCGCATTGAAGAAAGA+GGG + chr1.3:8668428-8668447 None:intergenic 40.0%
ATTAACAGTGTCCTTGTGTC+AGG + chr1.3:8668761-8668780 None:intergenic 40.0%
CAATGTTCATATGCCAGACA+TGG - chr1.3:8667865-8667884 MS.gene33157:CDS 40.0%
GTTGTTGCGGTCAATCAATT+AGG - chr1.3:8668384-8668403 MS.gene33157:intron 40.0%
TATTGGAATGCGAGTTCTTG+CGG - chr1.3:8667165-8667184 MS.gene33157:intron 40.0%
TCATTTGGCAGCATGTAATC+AGG - chr1.3:8668127-8668146 MS.gene33157:intron 40.0%
TCTACAAAGAATTAGCCGTG+CGG - chr1.3:8668998-8669017 MS.gene33157:intron 40.0%
TGACACAAGGACACTGTTAA+TGG - chr1.3:8668760-8668779 MS.gene33157:intron 40.0%
TGACAGTGAGGAAGAAAATG+AGG - chr1.3:8668284-8668303 MS.gene33157:CDS 40.0%
TGCATCTGAACTACTAGCTA+AGG + chr1.3:8669051-8669070 None:intergenic 40.0%
TGCGAATGATGATCCAAAGA+TGG - chr1.3:8668035-8668054 MS.gene33157:CDS 40.0%
TGGAGAGTGAAACAATGTAG+AGG + chr1.3:8666996-8667015 None:intergenic 40.0%
TGTCTTTGTCGTGTTAGTGT+CGG - chr1.3:8668878-8668897 MS.gene33157:intron 40.0%
TGTTGATGTCGTGTTAGTGT+CGG - chr1.3:8668818-8668837 MS.gene33157:intron 40.0%
! ATGTGAGACTGAGAAGTATG+GGG + chr1.3:8669123-8669142 None:intergenic 40.0%
! GATCCAAAGATGGTGATGAA+AGG - chr1.3:8668045-8668064 MS.gene33157:CDS 40.0%
!! ACATCGCAAGTTTGTTGTTG+CGG - chr1.3:8668371-8668390 MS.gene33157:intron 40.0%
!! GGTGATCTTACGTTGTTTCT+TGG - chr1.3:8667657-8667676 MS.gene33157:intron 40.0%
!! GTTGATGATGAAGCATACCA+TGG + chr1.3:8667249-8667268 None:intergenic 40.0%
!! TAAAAGTACCTGAAGATGGC+TGG + chr1.3:8668687-8668706 None:intergenic 40.0%
!! TACCATGGTATTGACACCTT+TGG + chr1.3:8667234-8667253 None:intergenic 40.0%
!!! CTAGGGTTTTGGCTAAGTTT+TGG - chr1.3:8667366-8667385 MS.gene33157:intron 40.0%
ACATGGTGCGTGTGATTATC+TGG - chr1.3:8668074-8668093 MS.gene33157:CDS 45.0%
AGAAGAAGCCTCGAACTGTT+TGG - chr1.3:8668337-8668356 MS.gene33157:CDS 45.0%
CACTGCTCAAAACGCGATAA+CGG - chr1.3:8667793-8667812 MS.gene33157:CDS 45.0%
CCTATAATGAGCCTGACACA+AGG - chr1.3:8668747-8668766 MS.gene33157:intron 45.0%
CCTTGTGTCAGGCTCATTAT+AGG + chr1.3:8668750-8668769 None:intergenic 45.0%
GAAATGGGAAAGATTCGGCT+GGG - chr1.3:8668202-8668221 MS.gene33157:intron 45.0%
GCAAGAACTCGCATTCCAAT+AGG + chr1.3:8667166-8667185 None:intergenic 45.0%
GGAAGACAGACAAGACCATA+TGG - chr1.3:8669019-8669038 MS.gene33157:intron 45.0%
GGTGGGAAAAGAATGTCACT+GGG + chr1.3:8669100-8669119 None:intergenic 45.0%
GTTCATATGCCAGACATGGA+TGG - chr1.3:8667869-8667888 MS.gene33157:CDS 45.0%
TAGCTAAGGCTGCTTCCATA+TGG + chr1.3:8669037-8669056 None:intergenic 45.0%
! TGTGAGACTGAGAAGTATGG+GGG + chr1.3:8669122-8669141 None:intergenic 45.0%
!! AGATTGGCTGCTGAGTTGAT+AGG + chr1.3:8669075-8669094 None:intergenic 45.0%
!! CTCCAAAGGTGTCAATACCA+TGG - chr1.3:8667229-8667248 MS.gene33157:intron 45.0%
!! TGAGCTTGTTGGACTTGAGA+TGG - chr1.3:8667904-8667923 MS.gene33157:CDS 45.0%
GACGCGCCTAATTCTGATAG+TGG - chr1.3:8668180-8668199 MS.gene33157:intron 50.0%
GGAAATGGGAAAGATTCGGC+TGG - chr1.3:8668201-8668220 MS.gene33157:intron 50.0%
GGGTGGGAAAAGAATGTCAC+TGG + chr1.3:8669101-8669120 None:intergenic 50.0%
TCAGTGGACCAAACAGTTCG+AGG + chr1.3:8668348-8668367 None:intergenic 50.0%
! GTGAGACTGAGAAGTATGGG+GGG + chr1.3:8669121-8669140 None:intergenic 50.0%
!! CGTTATCGCGTTTTGAGCAG+TGG + chr1.3:8667795-8667814 None:intergenic 50.0%
AAAGGTGGCCAGCCATCTTC+AGG - chr1.3:8668676-8668695 MS.gene33157:intron 55.0%
! AGACTGAGAAGTATGGGGGG+TGG + chr1.3:8669118-8669137 None:intergenic 55.0%
! GACTGAGAAGTATGGGGGGT+GGG + chr1.3:8669117-8669136 None:intergenic 55.0%
! GGTCTTGTCTGTCTTCCGCA+CGG + chr1.3:8669016-8669035 None:intergenic 55.0%
GGACGCAGAATGCACAGCGT+CGG - chr1.3:8668839-8668858 MS.gene33157:intron 60.0%
GGGAAAGATTCGGCTGGGAC+AGG - chr1.3:8668207-8668226 MS.gene33157:CDS 60.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 8666988 8669160 8666988 ID=MS.gene33157
chr1.3 mRNA 8666988 8669160 8666988 ID=MS.gene33157.t1;Parent=MS.gene33157
chr1.3 exon 8669119 8669160 8669119 ID=MS.gene33157.t1.exon1;Parent=MS.gene33157.t1
chr1.3 CDS 8669119 8669160 8669119 ID=cds.MS.gene33157.t1;Parent=MS.gene33157.t1
chr1.3 exon 8668898 8669000 8668898 ID=MS.gene33157.t1.exon2;Parent=MS.gene33157.t1
chr1.3 CDS 8668898 8669000 8668898 ID=cds.MS.gene33157.t1;Parent=MS.gene33157.t1
chr1.3 exon 8668207 8668359 8668207 ID=MS.gene33157.t1.exon3;Parent=MS.gene33157.t1
chr1.3 CDS 8668207 8668359 8668207 ID=cds.MS.gene33157.t1;Parent=MS.gene33157.t1
chr1.3 exon 8667734 8668120 8667734 ID=MS.gene33157.t1.exon4;Parent=MS.gene33157.t1
chr1.3 CDS 8667734 8668120 8667734 ID=cds.MS.gene33157.t1;Parent=MS.gene33157.t1
chr1.3 exon 8667451 8667527 8667451 ID=MS.gene33157.t1.exon5;Parent=MS.gene33157.t1
chr1.3 CDS 8667451 8667527 8667451 ID=cds.MS.gene33157.t1;Parent=MS.gene33157.t1
chr1.3 exon 8666988 8667164 8666988 ID=MS.gene33157.t1.exon6;Parent=MS.gene33157.t1
chr1.3 CDS 8666988 8667164 8666988 ID=cds.MS.gene33157.t1;Parent=MS.gene33157.t1
Gene Sequence

>MS.gene33157

ATGATCCTCTACATTGTTTCACTCTCCATAGTAACTTTCATGGTACCTTCTTTTTTCCATATATACTTTACAACATTCTTTTCTTAATCATTCTATCTTTGCATTCATCACTTGTTATTTTTCTCTTTGTCAACGAAAATTCTTCCTTAAAAATCACAAGATGAATGATCAGTTTCCTATTGGAATGCGAGTTCTTGCGGTTGATGATGATAGAACATGTCTCAAGATTTTGGAGAAACTTCTCCAAAGGTGTCAATACCATGGTATGCTTCATCATCAACATCATATTATTGTTGTTTTTGTTTTATTTTGAAATATTAAAGATTGGTCCTTGATTTTAATCTTGTTTTTTGATGTGACAAATCTAATGATATATAGCTAGGGTTTTGGCTAAGTTTTGGATACATATATTTATGCATATGTTAGATTATTATTTTCAATGAAGTTGGTTCATTTTGAATCAAATATCTATTTTTCAATATTATGTTCAACACTACATCCAATGCTTGTAGAAATCAATTAGATTAATTTTAAATTTTATTTGATATTGTTTAGGTTTAGACTAAATTATATTTGATCGATCACATTCTCGATTCTTTCAAAGATTGGACATGATTTTGCTTTTAAAGTTGCAATTTTTTTTAAATATCTTTTGTGTGCCTAATCTTTGGTGATCTTACGTTGTTTCTTGGATTTCAAAACATTTCGTAGATTTTATGTTCTGATTGTATATGCAGTGTATAATTTTTTTCTCTTTGAGAATGATATTAATTTTAGTTATGGTTTGTTAATTTGTTTCAGTAACCACTGCTCAAAACGCGATAACGGCGTTGAACTTGTTGAGAGAAAACAAAAGCAATTTTGACCTAGTAATCAGCAATGTTCATATGCCAGACATGGATGGATTTAAGCTTCTTGAGCTTGTTGGACTTGAGATGGACCTACCTGTCATAAGTAAGTTATGCAATTCCTTCAATCTTATATTATTGATACATGTATAACAAATTATAGTATTTTGATTGCAAAGTGTTTGATTTCAGTGTTTTCTGCGAATGATGATCCAAAGATGGTGATGAAAGGAATTGAACATGGTGCGTGTGATTATCTGGTGAAACCTGTTAGACTAAAAGAGGTACAGATCATTTGGCAGCATGTAATCAGGAAGAAGAAAACTAGCAAAAGAAGCAATCACGACGCGCCTAATTCTGATAGTGGAAATGGGAAAGATTCGGCTGGGACAGGAAATTCTGATAAGAATGAAAAACCATCTAGAAAAAGGAAGGATAAGAATGAAGATGACAGTGAGGAAGAAAATGAGGATGATTATGACAATGATGATCAAACTGCGCAGAAGAAGCCTCGAACTGTTTGGTCCACTGATTTACATCGCAAGTTTGTTGTTGCGGTCAATCAATTAGGCATTGACGGTACGTTCTTCCCTCTTTCTTCAATGCGCAGATTATAACGATCCAATGTTGAGATTAATATCATGATATAAAAATAAAAATTCTACTTTGTATTATTTGAAGACTTCCTCAAATATTCTTCCCGAAAAATACTTATGTTTAGCCATGCTATGAATTTTTCCGATTCTGAAGTGTTTTTCTCATGATAGTTGTTATCTTACTTGCAGAGGCTGTACCAAAAAAGATTCTTGAACTGATGAATGTTGAAAATCTGACAAGGGAAAAGGTGGCCAGCCATCTTCAGGTACTTTTATGCTTTAGCGTTATAAGTTACGAAGATATAGACACGTAAACCTATAATGAGCCTGACACAAGGACACTGTTAATGGTTTGATTGAATGAAGTAATTGAATTTAATCACATATGTTGATGTCGTGTTAGTGTCGGACGCAGAATGCACAGCGTCGGTGCTACACAAATTATCTGTCTTTGTCGTGTTAGTGTCGGACGCAGAATGCACAGCGTCGGTGCTACACAAATTATCTGTCTTTTCATTCAAGAAAACTTACTGTTTTTTAATTTTTCTGCAGAAGTATAGACTCTATCTACAAAGAATTAGCCGTGCGGAAGACAGACAAGACCATATGGAAGCAGCCTTAGCTAGTAGTTCAGATGCATCCTATCAACTCAGCAGCCAATCTTTCCCAGTGACATTCTTTTCCCACCCCCCATACTTCTCAGTCTCACATATCTTTCATGATTTGTAA
Protein sequence

>MS.gene33157.t1

MILYIVSLSIVTFMMNDQFPIGMRVLAVDDDRTCLKILEKLLQRCQYHVTTAQNAITALNLLRENKSNFDLVISNVHMPDMDGFKLLELVGLEMDLPVIMFSANDDPKMVMKGIEHGACDYLVKPVRLKEVQIIWQHVIRKKKTSKRSNHDAPNSDSGNGKDSAGTGNSDKNEKPSRKRKDKNEDDSEEENEDDYDNDDQTAQKKPRTVWSTDLHRKFVVAVNQLGIDEAVPKKILELMNVENLTREKVASHLQKYRLYLQRISRAEDRQDHMEAALASSSDASYQLSSQSFPVTFFSHPPYFSVSHIFHDL