AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene33545

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene33545.t1 MTR_4g131600 75.709 247 7 1 1 194 4 250 6.45e-123 359
MS.gene33545.t1 MTR_4g131580 60.241 249 44 2 1 194 1 249 3.47e-91 278
MS.gene33545.t1 MTR_4g131570 67.614 176 56 1 1 175 1 176 1.76e-74 235
MS.gene33545.t1 MTR_4g131570 80.435 46 9 0 149 194 207 252 1.80e-17 80.1
MS.gene33545.t1 MTR_3g102600 59.783 184 70 1 11 194 2 181 8.73e-74 221
MS.gene33545.t1 MTR_3g102600 59.783 184 70 1 11 194 2 181 8.02e-73 219
MS.gene33545.t1 MTR_3g086100 50.000 236 60 2 17 194 13 248 6.70e-70 222
MS.gene33545.t1 MTR_1g032570 47.699 239 64 2 17 194 11 249 1.98e-68 219
MS.gene33545.t1 MTR_8g079940 49.576 236 63 2 15 194 23 258 1.13e-67 216
MS.gene33545.t1 MTR_2g034960 47.899 238 70 2 11 194 23 260 4.60e-67 216
MS.gene33545.t1 MTR_3g102590 59.459 185 68 2 17 194 8 192 3.98e-66 201
MS.gene33545.t1 MTR_3g102590 59.459 185 68 2 17 194 8 192 9.14e-66 202
MS.gene33545.t1 MTR_7g117705 48.333 240 66 3 13 194 1 240 1.59e-65 208
MS.gene33545.t1 MTR_1g013180 49.167 240 64 2 13 194 152 391 2.20e-65 213
MS.gene33545.t1 MTR_3g102600 61.029 136 49 1 59 194 1 132 5.85e-56 174
MS.gene33545.t1 MTR_3g106220 65.693 137 46 1 14 149 5 141 1.02e-55 186
MS.gene33545.t1 MTR_3g106220 76.087 46 11 0 149 194 202 247 1.49e-16 77.4
MS.gene33545.t1 MTR_2g450070 62.838 148 54 1 2 148 3 150 1.92e-55 186
MS.gene33545.t1 MTR_2g450070 73.913 46 12 0 149 194 217 262 5.75e-17 78.6
MS.gene33545.t1 MTR_2g450070 62.838 148 54 1 2 148 3 150 2.61e-55 186
MS.gene33545.t1 MTR_2g450070 73.913 46 12 0 149 194 217 262 6.13e-17 78.6
MS.gene33545.t1 MTR_3g102600 61.029 136 49 1 59 194 1 132 4.91e-55 172
MS.gene33545.t1 MTR_1g013170 63.235 136 50 0 13 148 1 136 2.50e-54 180
MS.gene33545.t1 MTR_1g013170 73.913 46 12 0 149 194 195 240 1.40e-14 71.6
MS.gene33545.t1 MTR_4g121020 66.154 130 41 1 14 140 25 154 2.00e-53 181
MS.gene33545.t1 MTR_4g121020 71.739 46 13 0 149 194 219 264 1.52e-14 71.6
MS.gene33545.t1 MTR_4g121020 66.154 130 41 1 14 140 25 154 2.04e-53 181
MS.gene33545.t1 MTR_4g121020 71.739 46 13 0 149 194 219 264 1.51e-14 71.6
MS.gene33545.t1 MTR_4g021760 36.190 210 102 2 16 194 10 218 6.41e-45 151
MS.gene33545.t1 MTR_4g021845 38.095 210 98 3 16 194 9 217 7.69e-45 148
MS.gene33545.t1 MTR_4g021790 38.182 220 101 4 6 194 2 217 2.04e-43 146
MS.gene33545.t1 MTR_1g032570 45.000 180 38 2 76 194 1 180 2.58e-43 152
MS.gene33545.t1 MTR_4g021855 37.619 210 99 2 16 194 9 217 7.85e-43 145
MS.gene33545.t1 MTR_3g086100 44.633 177 40 2 76 194 1 177 1.49e-40 144
MS.gene33545.t1 MTR_6g045327 33.645 214 112 2 11 194 8 221 5.75e-38 135
MS.gene33545.t1 MTR_5g055260 37.696 191 98 6 16 194 9 190 1.58e-33 120
MS.gene33545.t1 MTR_0450s0040 32.864 213 106 2 19 194 6 218 9.36e-32 117
MS.gene33545.t1 MTR_8g105600 33.047 233 104 4 13 194 5 236 2.17e-31 119
MS.gene33545.t1 MTR_1g013160 65.714 70 24 0 79 148 1 70 1.71e-27 102
MS.gene33545.t1 MTR_7g026400 33.684 190 84 2 47 194 17 206 2.20e-26 105
MS.gene33545.t1 MTR_8g093040 30.732 205 111 4 13 194 10 206 1.44e-24 97.4
MS.gene33545.t1 MTR_6g016850 34.532 139 88 2 13 149 5 142 2.82e-24 94.0
MS.gene33545.t1 MTR_3g092780 37.008 127 75 2 21 143 56 181 1.09e-23 97.8
MS.gene33545.t1 MTR_3g092780 37.008 127 75 2 21 143 56 181 1.11e-23 97.8
MS.gene33545.t1 MTR_3g092780 36.220 127 76 2 21 143 56 181 5.44e-23 95.9
MS.gene33545.t1 MTR_3g092780 36.220 127 76 2 21 143 56 181 5.49e-23 95.9
MS.gene33545.t1 MTR_7g118260 36.090 133 76 2 18 144 11 140 6.50e-23 95.5
MS.gene33545.t1 MTR_7g118260 36.154 130 74 2 18 141 11 137 1.96e-21 91.3
MS.gene33545.t1 MTR_4g023980 32.117 137 62 1 63 168 35 171 1.50e-20 84.7
MS.gene33545.t1 MTR_4g061360 37.705 122 73 1 21 139 89 210 8.17e-20 87.0
MS.gene33545.t1 MTR_1g067110 34.426 122 77 1 21 139 85 206 9.77e-19 83.6
MS.gene33545.t1 MTR_1g067110 34.426 122 77 1 21 139 85 206 9.82e-19 83.6
MS.gene33545.t1 MTR_3g037390 31.579 133 86 2 13 140 14 146 4.28e-18 81.6
MS.gene33545.t1 MTR_8g024260 33.600 125 80 1 23 144 38 162 5.14e-18 81.6
MS.gene33545.t1 MTR_4g108880 30.303 132 87 2 14 140 24 155 1.47e-17 80.1
MS.gene33545.t1 MTR_5g014040 35.971 139 82 4 9 144 5 139 2.10e-17 79.7
MS.gene33545.t1 MTR_5g014040 62.222 45 17 0 150 194 316 360 1.05e-11 63.2
MS.gene33545.t1 MTR_5g014040 36.923 130 76 3 17 144 14 139 2.15e-17 79.7
MS.gene33545.t1 MTR_5g014040 62.222 45 17 0 150 194 316 360 9.99e-12 63.2
MS.gene33545.t1 MTR_8g032710 30.952 126 86 1 19 143 19 144 5.59e-17 78.6
MS.gene33545.t1 MTR_4g098870 33.594 128 81 2 17 143 14 138 1.31e-15 74.7
MS.gene33545.t1 MTR_4g098870 66.667 45 15 0 150 194 315 359 9.97e-13 66.2
MS.gene33545.t1 MTR_4g098870 33.594 128 81 2 17 143 34 158 1.39e-15 74.3
MS.gene33545.t1 MTR_4g098870 66.667 45 15 0 150 194 335 379 9.88e-13 66.2
MS.gene33545.t1 MTR_4g098870 33.594 128 81 2 17 143 14 138 1.51e-15 74.3
MS.gene33545.t1 MTR_4g098870 66.667 45 15 0 150 194 315 359 1.04e-12 66.2
MS.gene33545.t1 MTR_4g098870 33.594 128 81 2 17 143 53 177 1.71e-15 74.3
MS.gene33545.t1 MTR_4g098870 66.667 45 15 0 150 194 354 398 1.05e-12 66.2
MS.gene33545.t1 MTR_4g098870 33.594 128 81 2 17 143 34 158 1.73e-15 74.3
MS.gene33545.t1 MTR_4g098870 66.667 45 15 0 150 194 335 379 1.07e-12 66.2
MS.gene33545.t1 MTR_4g121020 71.739 46 13 0 149 194 115 160 1.59e-14 71.6
MS.gene33545.t1 MTR_4g121020 63.636 44 16 0 97 140 7 50 1.44e-13 68.6
MS.gene33545.t1 MTR_1874s0010 31.405 121 79 2 21 139 4 122 1.21e-13 67.4
MS.gene33545.t1 MTR_6g088950 35.106 94 61 0 47 140 34 127 4.84e-13 65.5
MS.gene33545.t1 MTR_7g490310 27.607 163 95 3 13 154 1 161 2.01e-12 63.9
MS.gene33545.t1 MTR_4g064730 47.826 69 36 0 126 194 112 180 4.28e-12 63.9
MS.gene33545.t1 MTR_5g014040 62.222 45 17 0 150 194 276 320 9.05e-12 63.5
MS.gene33545.t1 MTR_7g089010 58.696 46 19 0 149 194 95 140 4.35e-11 60.8
MS.gene33545.t1 MTR_4g078810 55.556 45 20 0 150 194 163 207 4.42e-11 61.2
MS.gene33545.t1 MTR_2g090675 55.556 45 20 0 150 194 166 210 8.12e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene33545.t1 AT2G25180 53.879 232 53 2 17 194 13 244 1.39e-72 230
MS.gene33545.t1 AT4G31920 54.545 220 58 2 17 194 13 232 1.96e-70 223
MS.gene33545.t1 AT5G58080 50.826 242 64 4 7 194 3 243 3.79e-64 209
MS.gene33545.t1 AT1G67710 67.188 128 41 1 16 142 5 132 1.21e-54 181
MS.gene33545.t1 AT1G67710 74.468 47 11 1 149 194 197 243 3.30e-14 70.5
MS.gene33545.t1 AT3G16857 60.902 133 52 0 8 140 24 156 1.28e-49 170
MS.gene33545.t1 AT3G16857 71.739 46 13 0 149 194 241 286 5.66e-15 72.8
MS.gene33545.t1 AT3G16857 60.902 133 52 0 8 140 24 156 1.99e-49 170
MS.gene33545.t1 AT3G16857 71.739 46 13 0 149 194 241 286 5.86e-15 72.8
MS.gene33545.t1 AT2G01760 58.915 129 52 1 13 140 3 131 1.02e-46 157
MS.gene33545.t1 AT2G01760 65.385 52 18 0 143 194 198 249 3.36e-15 73.2
MS.gene33545.t1 AT5G49240 34.440 241 101 4 11 194 32 272 9.38e-41 140
MS.gene33545.t1 AT2G01760 48.408 157 52 2 13 140 3 159 1.25e-40 142
MS.gene33545.t1 AT2G01760 65.385 52 18 0 143 194 226 277 3.16e-15 73.2
MS.gene33545.t1 AT3G16857 68.235 85 27 0 56 140 2 86 4.58e-34 127
MS.gene33545.t1 AT3G16857 71.739 46 13 0 149 194 171 216 5.94e-15 72.8
MS.gene33545.t1 AT3G62670 34.802 227 91 5 22 194 42 265 1.56e-27 107
MS.gene33545.t1 AT1G67710 70.149 67 20 0 76 142 1 67 2.99e-27 107
MS.gene33545.t1 AT1G67710 74.468 47 11 1 149 194 132 178 2.95e-14 70.5
MS.gene33545.t1 AT2G01760 66.667 66 21 1 76 140 1 66 2.77e-24 97.4
MS.gene33545.t1 AT2G01760 65.385 52 18 0 143 194 133 184 3.15e-15 72.8
MS.gene33545.t1 AT1G49190 36.800 125 79 0 17 141 30 154 7.20e-24 97.4
MS.gene33545.t1 AT1G49190 36.800 125 79 0 17 141 30 154 1.44e-23 97.8
MS.gene33545.t1 AT5G24470 33.094 139 88 2 20 154 49 186 4.29e-22 93.2
MS.gene33545.t1 AT2G46790 38.211 123 71 2 21 139 37 158 5.90e-22 92.4
MS.gene33545.t1 AT2G46790 38.211 123 71 2 21 139 37 158 6.56e-22 92.4
MS.gene33545.t1 AT5G60100 37.705 122 73 1 21 139 64 185 1.98e-19 85.1
MS.gene33545.t1 AT5G60100 37.705 122 73 1 21 139 64 185 1.98e-19 85.1
MS.gene33545.t1 AT5G60100 37.705 122 73 1 21 139 64 185 1.98e-19 85.1
MS.gene33545.t1 AT5G60100 37.705 122 73 1 21 139 64 185 3.26e-19 85.1
MS.gene33545.t1 AT5G60100 37.705 122 73 1 21 139 64 185 4.76e-19 84.3
MS.gene33545.t1 AT5G60100 37.705 122 73 1 21 139 64 185 4.76e-19 84.3
MS.gene33545.t1 AT5G60100 37.705 122 73 1 21 139 64 185 4.76e-19 84.3
MS.gene33545.t1 AT5G61380 31.818 132 85 2 14 140 10 141 1.39e-17 80.5
MS.gene33545.t1 AT5G02810 34.426 122 77 1 21 139 78 199 6.38e-17 78.6
MS.gene33545.t1 AT4G18020 34.286 140 83 3 15 147 12 149 1.20e-14 72.0
MS.gene33545.t1 AT4G18020 34.286 140 83 3 15 147 12 149 1.20e-14 72.0
MS.gene33545.t1 AT4G18020 34.286 140 83 3 15 147 12 149 1.20e-14 72.0
MS.gene33545.t1 AT4G18020 34.286 140 83 3 15 147 12 149 1.20e-14 72.0
MS.gene33545.t1 AT4G18020 34.286 140 83 3 15 147 12 149 1.20e-14 72.0
MS.gene33545.t1 AT4G18020 34.286 140 83 3 15 147 12 149 1.26e-14 71.6
MS.gene33545.t1 AT4G18020 34.286 140 83 3 15 147 12 149 1.26e-14 71.6
MS.gene33545.t1 AT4G18020 34.286 140 83 3 15 147 12 149 1.26e-14 71.6
MS.gene33545.t1 AT4G18020 34.286 140 83 3 15 147 12 149 1.26e-14 71.6
MS.gene33545.t1 AT4G18020 34.286 140 83 3 15 147 12 149 1.26e-14 71.6
MS.gene33545.t1 AT4G18020 34.286 140 83 3 15 147 12 149 1.26e-14 71.6
MS.gene33545.t1 AT1G67710 74.468 47 11 1 149 194 123 169 2.76e-14 70.9
MS.gene33545.t1 AT1G67710 60.870 46 18 0 97 142 13 58 1.54e-13 68.6
MS.gene33545.t1 AT5G07210 32.000 125 82 2 21 144 16 138 9.60e-14 69.3
MS.gene33545.t1 AT1G19050 26.590 173 103 5 7 158 1 170 2.53e-12 63.5
MS.gene33545.t1 AT5G44190 64.444 45 16 0 150 194 152 196 2.70e-12 64.7
MS.gene33545.t1 AT5G44190 64.444 45 16 0 150 194 152 196 2.92e-12 64.7
MS.gene33545.t1 AT5G59570 57.692 52 22 0 143 194 139 190 4.31e-12 63.9
MS.gene33545.t1 AT5G59570 57.692 52 22 0 143 194 139 190 4.31e-12 63.9
MS.gene33545.t1 AT2G40970 60.870 46 18 0 149 194 109 154 4.43e-12 63.2
MS.gene33545.t1 AT3G46640 60.870 46 18 0 149 194 148 193 1.40e-11 62.4
MS.gene33545.t1 AT3G46640 60.870 46 18 0 149 194 148 193 1.40e-11 62.4
MS.gene33545.t1 AT3G46640 60.870 46 18 0 149 194 148 193 1.45e-11 62.4
MS.gene33545.t1 AT5G05090 58.696 46 19 0 149 194 85 130 2.13e-11 61.6
MS.gene33545.t1 AT5G07210 30.075 133 82 3 21 144 16 146 3.41e-11 62.0
MS.gene33545.t1 AT3G10760 58.696 46 19 0 149 194 109 154 3.52e-11 61.2
MS.gene33545.t1 AT2G20570 62.222 45 17 0 150 194 174 218 3.69e-11 61.6
MS.gene33545.t1 AT2G20570 62.222 45 17 0 150 194 158 202 4.07e-11 61.6

Find 39 sgRNAs with CRISPR-Local

Find 152 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCGGCGGGTATGCGTGTTC+TGG 0.339385 8.2:+3645489 MS.gene33545:CDS
CTTCTCACATGCAACTATAA+TGG 0.375217 8.2:+3645556 MS.gene33545:CDS
GTTTCTGCAGTTAATCTTGT+CGG 0.381222 8.2:+3647933 MS.gene33545:CDS
TCAGCAAGTCGGACTTGAAA+TGG 0.400458 8.2:+3647461 MS.gene33545:CDS
TCCAGCACTTTGAGATAAGT+TGG 0.404451 8.2:-3645526 None:intergenic
AAATGTATCCAGCCATTTGC+AGG 0.408981 8.2:+3648130 MS.gene33545:CDS
ATATAATTTGTACCTGCAAA+TGG 0.440246 8.2:-3648142 None:intergenic
TGAATTGTTGAGAGAAAAGA+AGG 0.450895 8.2:+3647377 MS.gene33545:CDS
AGCTTGAAGCCATCCATGTC+TGG 0.455651 8.2:-3647435 None:intergenic
ATTCTAGTTCATGATGGTTG+TGG 0.457111 8.2:+3645426 None:intergenic
TACTACAACTACGAAGTCAT+TGG 0.471545 8.2:+3647347 MS.gene33545:CDS
CTTGACTTGATGAATGTTGA+AGG 0.474751 8.2:+3648095 MS.gene33545:CDS
TTAAGCTCTTCCATTCGAAT+CGG 0.481435 8.2:-3647666 None:intergenic
AATTTGTACCTGCAAATGGC+TGG 0.488638 8.2:-3648138 None:intergenic
TGGAAGAGCTTAAGAGCATA+TGG 0.489491 8.2:+3647676 MS.gene33545:CDS
TGAATGTTGAAGGGCTTACA+AGG 0.497287 8.2:+3648105 MS.gene33545:CDS
TAGAAAGAAGATTGAAAGCA+AGG 0.500776 8.2:+3647710 MS.gene33545:CDS
TGTGTGATTTATAGTGTTGT+CGG 0.513795 8.2:+3647578 MS.gene33545:intron
GAAAGCAAGGATCAAAATCA+AGG 0.515217 8.2:+3647723 MS.gene33545:CDS
TCCAACTTATCTCAAAGTGC+TGG 0.528445 8.2:+3645525 MS.gene33545:CDS
TGCTAACATCACTAATTACA+AGG 0.543736 8.2:-3647410 None:intergenic
GCCAGAACACGCATACCCGC+CGG 0.553676 8.2:-3645490 None:intergenic
ATGGTTGTGGAGGATAGTTT+CGG 0.554649 8.2:+3645439 MS.gene33545:CDS
TTAGGAGAAGGTTTCCCGGC+GGG 0.561505 8.2:+3645475 MS.gene33545:CDS
CCAGAACACGCATACCCGCC+GGG 0.571213 8.2:-3645489 None:intergenic
TTTCAAGTCCGACTTGCTGA+AGG 0.572445 8.2:-3647458 None:intergenic
TTGACTTGATGAATGTTGAA+GGG 0.580486 8.2:+3648096 MS.gene33545:CDS
TTCAAGCTCCTTCAGCAAGT+CGG 0.589889 8.2:+3647450 MS.gene33545:CDS
CTAGTTCATGATGGTTGTGG+AGG 0.595087 8.2:+3645429 None:intergenic
TGATGTTAGCATGCCAGACA+TGG 0.598210 8.2:+3647422 MS.gene33545:CDS
TCTAGTGAAACCGATTCGAA+TGG 0.598674 8.2:+3647656 MS.gene33545:CDS
GGTAAATGATGATATAGAGA+AGG 0.617327 8.2:+3647599 MS.gene33545:CDS
GTCACATAAACTCACTGTCA+AGG 0.629956 8.2:-3647956 None:intergenic
TGTGTGAGCTTACTGATGAA+AGG 0.640199 8.2:-3647489 None:intergenic
GTTAGCATGCCAGACATGGA+TGG 0.657571 8.2:+3647426 MS.gene33545:CDS
TGAGCTTACTGATGAAAGGT+AGG 0.657693 8.2:-3647485 None:intergenic
GTGATGAAGAGTGTGATACA+TGG 0.658673 8.2:+3647621 MS.gene33545:CDS
GAATGTTGAAGGGCTTACAA+GGG 0.675399 8.2:+3648106 MS.gene33545:CDS
TTTAGGAGAAGGTTTCCCGG+CGG 0.790759 8.2:+3645474 MS.gene33545:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAAAAAATCTAAAACAAAA+GGG + chr8.2:3647055-3647074 MS.gene33545:intron 10.0%
!!! TTAAAAAAATCTAAAACAAA+AGG + chr8.2:3647054-3647073 MS.gene33545:intron 10.0%
!!! TTTTTATGATAGTAAATAAA+TGG - chr8.2:3646823-3646842 None:intergenic 10.0%
!!! TTTTTTCTTTTTTTTTTTTG+AGG - chr8.2:3646611-3646630 None:intergenic 10.0%
!! AAATAAATGGAAATTATCTT+CGG - chr8.2:3646810-3646829 None:intergenic 15.0%
!! CAATTTACTAAAAAAAACTA+AGG - chr8.2:3645738-3645757 None:intergenic 15.0%
!!! CTTAGTTTTTTTTAGTAAAT+TGG + chr8.2:3645736-3645755 MS.gene33545:intron 15.0%
!! AAATATCCGAATATAAGAAA+TGG - chr8.2:3647554-3647573 None:intergenic 20.0%
!! AATGATTATTACTGCTTTAT+TGG + chr8.2:3647121-3647140 MS.gene33545:intron 20.0%
!! ATAAGAAATGGATCTTAATT+AGG - chr8.2:3647542-3647561 None:intergenic 20.0%
!! GAAGAGACATTTAAATAAAA+AGG - chr8.2:3647287-3647306 None:intergenic 20.0%
!! TAAGATCCATTTCTTATATT+CGG + chr8.2:3647545-3647564 MS.gene33545:intron 20.0%
!! TGATGAATGATTCTTAAATA+TGG + chr8.2:3648008-3648027 MS.gene33545:intron 20.0%
!!! AAAAATTTGTTTGTTCAGTT+CGG + chr8.2:3645919-3645938 MS.gene33545:intron 20.0%
!!! AAACCTTTTTTTTTTCTTTG+AGG + chr8.2:3645697-3645716 MS.gene33545:intron 20.0%
!!! AGATGTGTTACAATTTTTTA+GGG - chr8.2:3646250-3646269 None:intergenic 20.0%
!!! ATGATTATTACTGCTTTATT+GGG + chr8.2:3647122-3647141 MS.gene33545:intron 20.0%
!!! ATTCTTGTTAGCAATATTTT+CGG - chr8.2:3647787-3647806 None:intergenic 20.0%
!!! TTTTTAGTAAATTGGTGATT+AGG + chr8.2:3645744-3645763 MS.gene33545:intron 20.0%
! AAAAAAATCACTAACATGTC+AGG - chr8.2:3647148-3647167 None:intergenic 25.0%
! AACTACAACCTATTTATCAT+AGG + chr8.2:3646460-3646479 MS.gene33545:intron 25.0%
! AAGTTACTCAATAGTTATAG+TGG - chr8.2:3645987-3646006 None:intergenic 25.0%
! ACACCAATAAAACTTATTTG+TGG + chr8.2:3645810-3645829 MS.gene33545:intron 25.0%
! AGTGTATATTTATGCTTCAT+AGG + chr8.2:3646550-3646569 MS.gene33545:intron 25.0%
! AGTTGAAGAAACTACATATT+AGG - chr8.2:3648045-3648064 None:intergenic 25.0%
! ATTGAATGAACGAAAAAGAA+AGG - chr8.2:3645589-3645608 None:intergenic 25.0%
! GTTCTAGTATGATTTAAACA+TGG - chr8.2:3646437-3646456 None:intergenic 25.0%
! TTACATAGCCTATGATAAAT+AGG - chr8.2:3646471-3646490 None:intergenic 25.0%
!! AATTTTCATATGAGAATTGC+TGG + chr8.2:3647237-3647256 MS.gene33545:intron 25.0%
!! ATCAAGTCAAGTATTTTCTT+AGG - chr8.2:3648086-3648105 None:intergenic 25.0%
!! GGTCTTTTATATAATAATGC+TGG + chr8.2:3647258-3647277 MS.gene33545:intron 25.0%
!! TTTTCTGCATCATTAAAAAC+CGG + chr8.2:3646293-3646312 MS.gene33545:intron 25.0%
!! TTTTTCCTTCTTTTTCTGAA+TGG + chr8.2:3647167-3647186 MS.gene33545:intron 25.0%
!!! CAAAACAGATTTTAAGAGAA+AGG - chr8.2:3647515-3647534 None:intergenic 25.0%
!!! GAGATGTGTTACAATTTTTT+AGG - chr8.2:3646251-3646270 None:intergenic 25.0%
!!! TTGAATAACTTTTCGATTTG+AGG + chr8.2:3646920-3646939 MS.gene33545:intron 25.0%
ACGCCTCAAAGAAAAAAAAA+AGG - chr8.2:3645703-3645722 None:intergenic 30.0%
ACTCAATAGTTATAGTGGTA+CGG - chr8.2:3645982-3646001 None:intergenic 30.0%
ACTTCTGTCTCGTAAAAAAT+CGG - chr8.2:3646343-3646362 None:intergenic 30.0%
ATAGGCTATGTAAATGTATC+CGG + chr8.2:3646478-3646497 MS.gene33545:intron 30.0%
ATCCTAATAGTGTTTCTCTA+AGG + chr8.2:3646068-3646087 MS.gene33545:intron 30.0%
ATGTAGAGAGTAGAAAAACA+AGG - chr8.2:3646180-3646199 None:intergenic 30.0%
CTTTGGCAAAAGAATGAATT+AGG - chr8.2:3646016-3646035 None:intergenic 30.0%
GGTAAATGATGATATAGAGA+AGG + chr8.2:3647599-3647618 MS.gene33545:CDS 30.0%
TAAACCTTGTATCAGATGAA+AGG - chr8.2:3646209-3646228 None:intergenic 30.0%
TAGAAACCACAACAAAACTA+GGG - chr8.2:3646154-3646173 None:intergenic 30.0%
TAGAAAGAAGATTGAAAGCA+AGG + chr8.2:3647710-3647729 MS.gene33545:CDS 30.0%
TCAAAACCACACCTAAAATT+GGG + chr8.2:3646364-3646383 MS.gene33545:intron 30.0%
TGAATTGTTGAGAGAAAAGA+AGG + chr8.2:3647377-3647396 MS.gene33545:CDS 30.0%
TGCTAACATCACTAATTACA+AGG - chr8.2:3647413-3647432 None:intergenic 30.0%
TGTAGAGAGTAGAAAAACAA+GGG - chr8.2:3646179-3646198 None:intergenic 30.0%
TGTGTGATTTATAGTGTTGT+CGG + chr8.2:3647578-3647597 MS.gene33545:intron 30.0%
TTAGAAACCACAACAAAACT+AGG - chr8.2:3646155-3646174 None:intergenic 30.0%
TTCTTCAACTATTATTGCAG+AGG + chr8.2:3648055-3648074 MS.gene33545:intron 30.0%
! AAAAAGAAAGGTACAAAGTG+AGG + chr8.2:3647821-3647840 MS.gene33545:intron 30.0%
! AATCAAGGTATCATTAGTGA+TGG + chr8.2:3647738-3647757 MS.gene33545:intron 30.0%
! ATCAAGGTATCATTAGTGAT+GGG + chr8.2:3647739-3647758 MS.gene33545:intron 30.0%
! TAAATCACTAGCTTGATTCT+TGG + chr8.2:3647309-3647328 MS.gene33545:intron 30.0%
! TTGACTTGATGAATGTTGAA+GGG + chr8.2:3648096-3648115 MS.gene33545:CDS 30.0%
! TTTTGCCATTCAGAAAAAGA+AGG - chr8.2:3647175-3647194 None:intergenic 30.0%
!! GCTCCACAAATAAGTTTTAT+TGG - chr8.2:3645816-3645835 None:intergenic 30.0%
!! TTGGAAGTAGCTTTGATATA+AGG + chr8.2:3645674-3645693 MS.gene33545:intron 30.0%
AAAAGAAGCCTCGTTTAGTT+TGG + chr8.2:3647886-3647905 MS.gene33545:intron 35.0%
AACGCATGGACAAAAAAGAA+AGG + chr8.2:3647809-3647828 MS.gene33545:intron 35.0%
ACTATTAGGATTGAGTCTCT+TGG - chr8.2:3646059-3646078 None:intergenic 35.0%
CACCTTAGAGAAACACTATT+AGG - chr8.2:3646073-3646092 None:intergenic 35.0%
CACTAATACATATCAGACTC+CGG - chr8.2:3646500-3646519 None:intergenic 35.0%
CTCAAAACCACACCTAAAAT+TGG + chr8.2:3646363-3646382 MS.gene33545:intron 35.0%
CTTCTCACATGCAACTATAA+TGG + chr8.2:3645556-3645575 MS.gene33545:CDS 35.0%
GAAAGCAAGGATCAAAATCA+AGG + chr8.2:3647723-3647742 MS.gene33545:CDS 35.0%
GCAACGGTGTAAACATATAT+TGG + chr8.2:3645655-3645674 MS.gene33545:intron 35.0%
GCTAACAAGAATAAAACGCA+TGG + chr8.2:3647795-3647814 MS.gene33545:intron 35.0%
GTTTCTGCAGTTAATCTTGT+CGG + chr8.2:3647933-3647952 MS.gene33545:CDS 35.0%
TACTACAACTACGAAGTCAT+TGG + chr8.2:3647347-3647366 MS.gene33545:CDS 35.0%
TCTCTTTGCAACAAACACTA+GGG - chr8.2:3646119-3646138 None:intergenic 35.0%
TTAAGCTCTTCCATTCGAAT+CGG - chr8.2:3647669-3647688 None:intergenic 35.0%
TTCTCTTTGCAACAAACACT+AGG - chr8.2:3646120-3646139 None:intergenic 35.0%
! AAAGAAGCCTCGTTTAGTTT+GGG + chr8.2:3647887-3647906 MS.gene33545:intron 35.0%
! ATCATTAGTGATGGGGTATA+TGG + chr8.2:3647747-3647766 MS.gene33545:intron 35.0%
! CTTGACTTGATGAATGTTGA+AGG + chr8.2:3648095-3648114 MS.gene33545:CDS 35.0%
! GTTAGCAATATTTTCGGATG+AGG - chr8.2:3647781-3647800 None:intergenic 35.0%
! TCAAGGTATCATTAGTGATG+GGG + chr8.2:3647740-3647759 MS.gene33545:intron 35.0%
! TTTGAGGTAGCTTAGACATT+CGG - chr8.2:3646595-3646614 None:intergenic 35.0%
!!! GGTGATTAGGTTAGTTTTCA+CGG + chr8.2:3645757-3645776 MS.gene33545:intron 35.0%
!!! GTTAGTTTTCACGGTTTGAA+CGG + chr8.2:3645766-3645785 MS.gene33545:intron 35.0%
!!! TACAAACCCTAGTTTTGTTG+TGG + chr8.2:3646145-3646164 MS.gene33545:intron 35.0%
ACTATTCTCCTCTCATGCAA+CGG + chr8.2:3645639-3645658 MS.gene33545:intron 40.0%
ATCAAGCAACCAAAACAACG+TGG + chr8.2:3645842-3645861 MS.gene33545:intron 40.0%
ATGGTTGTGGAGGATAGTTT+CGG + chr8.2:3645439-3645458 MS.gene33545:CDS 40.0%
CAACTGATATGTGTGTGAGT+AGG - chr8.2:3646650-3646669 None:intergenic 40.0%
CTCACCTTTCATCTGATACA+AGG + chr8.2:3646202-3646221 MS.gene33545:intron 40.0%
GAATGTTGAAGGGCTTACAA+GGG + chr8.2:3648106-3648125 MS.gene33545:CDS 40.0%
GTCACATAAACTCACTGTCA+AGG - chr8.2:3647959-3647978 None:intergenic 40.0%
TCAAGCAACCAAAACAACGT+GGG + chr8.2:3645843-3645862 MS.gene33545:intron 40.0%
TCATGATCCCAAACTAAACG+AGG - chr8.2:3647897-3647916 None:intergenic 40.0%
TCCAACTTATCTCAAAGTGC+TGG + chr8.2:3645525-3645544 MS.gene33545:CDS 40.0%
TGAATGTTGAAGGGCTTACA+AGG + chr8.2:3648105-3648124 MS.gene33545:CDS 40.0%
TGAGCTTACTGATGAAAGGT+AGG - chr8.2:3647488-3647507 None:intergenic 40.0%
TGTGTGAGCTTACTGATGAA+AGG - chr8.2:3647492-3647511 None:intergenic 40.0%
TTGAGGTAGCTTAGACATTC+GGG - chr8.2:3646594-3646613 None:intergenic 40.0%
TTGCAACAAACACTAGGGTT+GGG - chr8.2:3646114-3646133 None:intergenic 40.0%
TTTGCAACAAACACTAGGGT+TGG - chr8.2:3646115-3646134 None:intergenic 40.0%
! AAGAAAGGTACAAAGTGAGG+AGG + chr8.2:3647824-3647843 MS.gene33545:intron 40.0%
! ACTAAACGAGGCTTCTTTTG+AGG - chr8.2:3647885-3647904 None:intergenic 40.0%
! GTGATGAAGAGTGTGATACA+TGG + chr8.2:3647621-3647640 MS.gene33545:CDS 40.0%
! TCTGAAACTGCCAAAATCGA+CGG - chr8.2:3646410-3646429 None:intergenic 40.0%
! TCTTTTGCCAAGACTGTTGT+GGG + chr8.2:3646703-3646722 MS.gene33545:intron 40.0%
! TGGAAGAGCTTAAGAGCATA+TGG + chr8.2:3647676-3647695 MS.gene33545:CDS 40.0%
! TGTTGTGGGTGTGTTTGTTT+TGG + chr8.2:3646717-3646736 MS.gene33545:intron 40.0%
! TTCTTTTGCCAAGACTGTTG+TGG + chr8.2:3646702-3646721 MS.gene33545:intron 40.0%
!! TCCAGCACTTTGAGATAAGT+TGG - chr8.2:3645529-3645548 None:intergenic 40.0%
!! TCTAGTGAAACCGATTCGAA+TGG + chr8.2:3647656-3647675 MS.gene33545:CDS 40.0%
!!! GTTGACGATGTTTTAGGAGA+AGG + chr8.2:3645463-3645482 MS.gene33545:CDS 40.0%
!!! TGTTTTAGGAGAAGGTTTCC+CGG + chr8.2:3645471-3645490 MS.gene33545:CDS 40.0%
!!! TTCGGTGTTGACGATGTTTT+AGG + chr8.2:3645457-3645476 MS.gene33545:CDS 40.0%
AAACACACCCACAACAGTCT+TGG - chr8.2:3646713-3646732 None:intergenic 45.0%
AGGATTGAGTCTCTTGGTCA+CGG - chr8.2:3646053-3646072 None:intergenic 45.0%
CCAATACTGACCTACTTTGG+AGG + chr8.2:3645943-3645962 MS.gene33545:intron 45.0%
CCTCCAAAGTAGGTCAGTAT+TGG - chr8.2:3645946-3645965 None:intergenic 45.0%
CGGTTTGAACGGTTGTGAAT+CGG + chr8.2:3645777-3645796 MS.gene33545:intron 45.0%
CTTGGTCACGGAAAGAGATT+CGG - chr8.2:3646041-3646060 None:intergenic 45.0%
GAAGTGAGAAAATCGGAGTC+CGG - chr8.2:3646315-3646334 None:intergenic 45.0%
GGTCCAATACTGACCTACTT+TGG + chr8.2:3645940-3645959 MS.gene33545:intron 45.0%
TCAGCAAGTCGGACTTGAAA+TGG + chr8.2:3647461-3647480 MS.gene33545:CDS 45.0%
TGATGTTAGCATGCCAGACA+TGG + chr8.2:3647422-3647441 MS.gene33545:CDS 45.0%
TGTTTACACCGTTGCATGAG+AGG - chr8.2:3645650-3645669 None:intergenic 45.0%
TTCAAGCTCCTTCAGCAAGT+CGG + chr8.2:3647450-3647469 MS.gene33545:CDS 45.0%
TTGGTCACGGAAAGAGATTC+GGG - chr8.2:3646040-3646059 None:intergenic 45.0%
! AGCAATCGAGGCCCAATTTT+AGG - chr8.2:3646378-3646397 None:intergenic 45.0%
! GACTCCGATTTTCTCACTTC+GGG + chr8.2:3646315-3646334 MS.gene33545:intron 45.0%
! GGACTCCGATTTTCTCACTT+CGG + chr8.2:3646314-3646333 MS.gene33545:intron 45.0%
! GTGTGAGTAGGTAGCTTTAC+TGG - chr8.2:3646638-3646657 None:intergenic 45.0%
!! TTTCAAGTCCGACTTGCTGA+AGG - chr8.2:3647461-3647480 None:intergenic 45.0%
!!! AAAATATTTTGTTAAAAAAA+TGG - chr8.2:3646958-3646977 None:intergenic 5.0%
!!! AAATTATATAAGTATTTTTT+TGG + chr8.2:3647005-3647024 MS.gene33545:intron 5.0%
AGCAACCAAAACAACGTGGG+TGG + chr8.2:3645846-3645865 MS.gene33545:intron 50.0%
AGCTTGAAGCCATCCATGTC+TGG - chr8.2:3647438-3647457 None:intergenic 50.0%
CGGAAAGAGATTCGGGACTT+TGG - chr8.2:3646033-3646052 None:intergenic 50.0%
GTTAGCATGCCAGACATGGA+TGG + chr8.2:3647426-3647445 MS.gene33545:CDS 50.0%
TTTAGGAGAAGGTTTCCCGG+CGG + chr8.2:3645474-3645493 MS.gene33545:CDS 50.0%
!! TCGAGGCCCAATTTTAGGTG+TGG - chr8.2:3646373-3646392 None:intergenic 50.0%
!!! TCCAAATCGGCCGTCGATTT+TGG + chr8.2:3646397-3646416 MS.gene33545:intron 50.0%
GCCAAAATCGACGGCCGATT+TGG - chr8.2:3646401-3646420 None:intergenic 55.0%
GCTGCTCTCTCCTCCAAAGT+AGG - chr8.2:3645956-3645975 None:intergenic 55.0%
GGCGCCCGAAGTGAGAAAAT+CGG - chr8.2:3646322-3646341 None:intergenic 55.0%
GGGCCTCGATTGCTCCAAAT+CGG + chr8.2:3646384-3646403 MS.gene33545:intron 55.0%
TTAGGAGAAGGTTTCCCGGC+GGG + chr8.2:3645475-3645494 MS.gene33545:CDS 55.0%
! AGCTGCCACCCACGTTGTTT+TGG - chr8.2:3645854-3645873 None:intergenic 55.0%
!! CGGCCGATTTGGAGCAATCG+AGG - chr8.2:3646390-3646409 None:intergenic 60.0%
CCAGAACACGCATACCCGCC+GGG - chr8.2:3645492-3645511 None:intergenic 65.0%
GCCAGAACACGCATACCCGC+CGG - chr8.2:3645493-3645512 None:intergenic 65.0%
CCCGGCGGGTATGCGTGTTC+TGG + chr8.2:3645489-3645508 MS.gene33545:CDS 70.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 3645439 3648151 3645439 ID=MS.gene33545
chr8.2 mRNA 3645439 3648151 3645439 ID=MS.gene33545.t1;Parent=MS.gene33545
chr8.2 exon 3645439 3645577 3645439 ID=MS.gene33545.t1.exon1;Parent=MS.gene33545.t1
chr8.2 CDS 3645439 3645577 3645439 ID=cds.MS.gene33545.t1;Parent=MS.gene33545.t1
chr8.2 exon 3647346 3647498 3647346 ID=MS.gene33545.t1.exon2;Parent=MS.gene33545.t1
chr8.2 CDS 3647346 3647498 3647346 ID=cds.MS.gene33545.t1;Parent=MS.gene33545.t1
chr8.2 exon 3647592 3647744 3647592 ID=MS.gene33545.t1.exon3;Parent=MS.gene33545.t1
chr8.2 CDS 3647592 3647744 3647592 ID=cds.MS.gene33545.t1;Parent=MS.gene33545.t1
chr8.2 exon 3647904 3647963 3647904 ID=MS.gene33545.t1.exon4;Parent=MS.gene33545.t1
chr8.2 CDS 3647904 3647963 3647904 ID=cds.MS.gene33545.t1;Parent=MS.gene33545.t1
chr8.2 exon 3648075 3648151 3648075 ID=MS.gene33545.t1.exon5;Parent=MS.gene33545.t1
chr8.2 CDS 3648075 3648151 3648075 ID=cds.MS.gene33545.t1;Parent=MS.gene33545.t1
Gene Sequence

>MS.gene33545

ATGGTTGTGGAGGATAGTTTCGGTGTTGACGATGTTTTAGGAGAAGGTTTCCCGGCGGGTATGCGTGTTCTGGCAGTTGATGACGATCCAACTTATCTCAAAGTGCTGGAGAAGCAACTTCTCACATGCAACTATAATGGTTCGTTGCCTTTCTTTTTCGTTCATTCAATATGATTACGCATTGATTATTCTTTATGTTTACTATTCTCCTCTCATGCAACGGTGTAAACATATATTGGAAGTAGCTTTGATATAAGGAAACCTTTTTTTTTTCTTTGAGGCGTTGATATAGTAAACCTTAGTTTTTTTTAGTAAATTGGTGATTAGGTTAGTTTTCACGGTTTGAACGGTTGTGAATCGGTTAAAAAATCACACCAATAAAACTTATTTGTGGAGCAAATGAATCAAGCAACCAAAACAACGTGGGTGGCAGCTCTAAACACAATCAAAAGATAGAACAACAAAAGAGAAAGAACACACAAAAATTTGTTTGTTCAGTTCGGTCCAATACTGACCTACTTTGGAGGAGAGAGCAGCTCTCCGTACCACTATAACTATTGAGTAACTTTACAAACCTAATTCATTCTTTTGCCAAAGTCCCGAATCTCTTTCCGTGACCAAGAGACTCAATCCTAATAGTGTTTCTCTAAGGTGAATCAATGATGACTCAATCCCAACCCTAGTGTTTGTTGCAAAGAGAAAACTCTACAAACCCTAGTTTTGTTGTGGTTTCTAAACCCTTGTTTTTCTACTCTCTACATCTCTCACCTTTCATCTGATACAAGGTTTATATTTATAGTAAAAGCATCCCTAAAAAATTGTAACACATCTCATGCTGAAAATACGTTTATGTTTTTTCTGCATCATTAAAAACCGGACTCCGATTTTCTCACTTCGGGCGCCGATTTTTTACGAGACAGAAGTCTCAAAACCACACCTAAAATTGGGCCTCGATTGCTCCAAATCGGCCGTCGATTTTGGCAGTTTCAGAAACACCATGTTTAAATCATACTAGAACATGAACTACAACCTATTTATCATAGGCTATGTAAATGTATCCGGAGTCTGATATGTATTAGTGTCTGATACAATTTGACGCATGTCATGTGTTAGTGTATATTTATGCTTCATAGGTTATAAGTCTTCTAGTGTCCCGAATGTCTAAGCTACCTCAAAAAAAAAAAAGAAAAAAACTACCAGTAAAGCTACCTACTCACACACATATCAGTTGACAATTTTCTTGATTTTAAATACATAAATTCATTCTTTTGCCAAGACTGTTGTGGGTGTGTTTGTTTTGGCTTAAAATAAAATTATTTTGTTTTTATAAAATATAAATATTATTTTTGAGATCTATAAAAAAAAAAGCCGAAGATAATTTCCATTTATTTACTATCATAAAAATAATTTTTTTAAGATTTAAAACACTTACACTTGAGTTTCTTGTTTTTTAAAAGAAAAATAGATTTTGAAAAAGCTATTTGAATAACTTTTCGATTTGAGGCTAAAAATTACTCCATTTTTTTAACAAAATATTTTTAAAAAAAATCTGAAACAAACACTATAAAATTATATAAGTATTTTTTTGGTTTAAAAAATTGATTTTTTGTTTCTTTTAAAAAAATCTAAAACAAAAGGGTCTGTGTTTTCTCTACTTCTAAAGTTGAAATTTTAAACTTTTTAATGATTATTACTGCTTTATTGGGCCTGACATGTTAGTGATTTTTTTTTTTCCTTCTTTTTCTGAATGGCAAAAGAAAATTTTATAAAATAAGATGATACTAATCTTATTTAATAAAATTTTCATATGAGAATTGCTGGTCTTTTATATAATAATGCTGGAAGCCTTTTTATTTAAATGTCTCTTCACTAAATCACTAGCTTGATTCTTGGTTGCATTGTTGCAGTTACTACAACTACGAAGTCATTGGAAGCGCTTGAATTGTTGAGAGAAAAGAAGGATATGTTTGACCTTGTAATTAGTGATGTTAGCATGCCAGACATGGATGGCTTCAAGCTCCTTCAGCAAGTCGGACTTGAAATGGACCTACCTTTCATCAGTAAGCTCACACACCTTTCTCTTAAAATCTGTTTTGTTTACCTAATTAAGATCCATTTCTTATATTCGGATATTTTGTGTGTGTGATTTATAGTGTTGTCGGTAAATGATGATATAGAGAAGGTGATGAAGAGTGTGATACATGGTGCTTGCAACTATCTAGTGAAACCGATTCGAATGGAAGAGCTTAAGAGCATATGGCAACATGTAGTTAGAAAGAAGATTGAAAGCAAGGATCAAAATCAAGGTATCATTAGTGATGGGGTATATGGTCAAGACACCTCATCCGAAAATATTGCTAACAAGAATAAAACGCATGGACAAAAAAGAAAGGTACAAAGTGAGGAGGAAGAAGCAGAACAAGATAATGATGAGAAACGTTCGCCTCAAAAGAAGCCTCGTTTAGTTTGGGATCATGAGTTGCATCGAAAATTTGTTTCTGCAGTTAATCTTGTCGGCCTTGACAGTGAGTTTATGTGACAATGAAATTATTTTTTTACTTTGCTAATTTGATGAATGATTCTTAAATATGGATATTATTGTTCCTAATATGTAGTTTCTTCAACTATTATTGCAGAGGCTTCGCCTAAGAAAATACTTGACTTGATGAATGTTGAAGGGCTTACAAGGGAAAATGTATCCAGCCATTTGCAG
Protein sequence

>MS.gene33545.t1

MVVEDSFGVDDVLGEGFPAGMRVLAVDDDPTYLKVLEKQLLTCNYNVTTTTKSLEALELLREKKDMFDLVISDVSMPDMDGFKLLQQVGLEMDLPFIMLSVNDDIEKVMKSVIHGACNYLVKPIRMEELKSIWQHVVRKKIESKDQNQVWDHELHRKFVSAVNLVGLDKASPKKILDLMNVEGLTRENVSSHLQ